CN101949886A - Real-time fluorescence electrophoresis apparatus - Google Patents
Real-time fluorescence electrophoresis apparatus Download PDFInfo
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- CN101949886A CN101949886A CN2010102670669A CN201010267066A CN101949886A CN 101949886 A CN101949886 A CN 101949886A CN 2010102670669 A CN2010102670669 A CN 2010102670669A CN 201010267066 A CN201010267066 A CN 201010267066A CN 101949886 A CN101949886 A CN 101949886A
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Abstract
The invention discloses a real-time fluorescence electrophoresis device, comprising an electrophoresis tank and a cover body, wherein, the electrophoresis tank comprises a platform, an electrophoresis buffer, a positive electrode and a negative electrode, wherein, the platform is loaded with a colloid with a biological sample; the colloid, the platform, the positive electrode and the negative electrode are soaked in the electrophoresis buffer; the cover body is contained above the electrophoresis tank, and comprises at least one light emitting component and an optical filter, wherein, the light emitting component is used for irradiating the colloid to lead the biological sample in the colloid to emit fluorescence, thus an experimenter can observe the fluorescent expression of the biological sample through the optical filter in the electrophoresis process, and can immediately know the electrophoretic progress, and determines whether the electrophoresis experiment is stopped or not, thus avoiding the error of the electrophoresis experiment.
Description
Technical field
The present invention is relevant for a kind of real-time fluorescence electrophoretic apparatus, and in electrophoresis process, the experimenter can observe the fluorescence performance of biological sample, learns the progress of electrophoresis immediately, and whether decision stops electrophoresis experiment, uses the mistake of avoiding electrophoresis experiment.
Background technology
Electrophoretic techniques often is used in biological sample (for example: DNA or protein) analytically, utilizes electrophoresis result can learn the every data of the molecular weight of DNA, purity or structure or the like.
Generally before carrying out the DNA electrophoresis, DNA can be placed in the colloid, its colloid electrophoresis mainly adopts dual mode, and a kind of is agar colloid electrophoresis (agarose gel electrophoresis; AGE), another kind is polyacrylamide colloid electrophoresis (polyacrylamide gel electrophoresis; PAGE), the former be used for the bigger nucleic acid of isolated molecule amount (for example: 1～60000bp), the latter is then in order to than the nucleic acid of micromolecule (for example: 1～1000bp) to separate.
When colloid electrophoresis, utilize the electronegative characteristic of dna molecular, under an effect of electric field, dna molecular can move in colloid, and advances towards positive pole, and each dna molecular can be because of the difference of molecular weight size, and its translational speed is difference to some extent.In addition; DNA in the colloid usually can be via stain (for example: EtBr) dyeing; utilize the light source irradiation of a specific wavelength again; after then dna molecular absorbs this wavelength light source; stain sends fluorescence; with the position of in electrophoresis process, being moved, and separate out different dna moleculars through the Fluirescence observation dna molecular.
As shown in Figure 1, be the structural representation of known electrophoretic apparatus.As shown in the figure, electrophoretic apparatus 100 includes a colloid 11 and the power supply unit 13 that an electrophoresis tank 10, has biological sample.
Wherein, this colloid 11 includes the charged molecule 111 of a plurality of biological samples (for example: dna molecular), and biological sample is through stain dyeing.Electrophoresis tank 10 includes a platform 101, an electrophoresis liquid 103, a positive electrode 105 and a negative electrode 107, and colloid 11 is put on platform 101, and colloid 11, platform 101, positive electrode 105 and negative electrode 107 are soaked in the electrophoresis liquid 103.And power supply unit 13 is a direct current power supply and is electrically connected to positive electrode 105 and negative electrode 107.
When power supply unit 13 power supplies, 107 of positive electrode 105 and negative electrodes can produce an electric field, electric field can drive charged molecule 111 in the colloid 11 respectively towards moving with its electrically opposite electrode 105/107, for example: when charged molecule 111 is negative charge, charged molecule 111 can move toward positive electrode 105, and charged molecule 111 is a positive charge, and then charged molecule 111 moves toward negative electrode 107 directions.Moreover, each charged molecule 111 can produce different translational speeds according to the molecular weight size, in other words, charged molecule 111 its translational speeds of larger molecular weight can be less than the charged molecule 111 than small-molecular weight, therefore, behind a suitable electrophoresis time, the shift length of charged molecule 111 in colloid 11 of different molecular weight is with different.
Afterwards, the colloid 11 of finishing electrophoresis takes out from electrophoresis tank 10, by light supply apparatus (not shown) irradiation, sends fluorescence to make the charged molecule 111 in the colloid 11, uses the change in displacement of observing each charged molecule 111, and separates out different dna moleculars.
As mentioned above, biological sample in the colloid 11 also can carry out electrophoresis by conventional electrophoretic device 100, so, in carrying out electrophoresis process, charged molecule 111 its speed that are subjected to electric field driven of all types of biological samples differ, therefore on identical colloid 11 sizes, finish the required time of electrophoresis experiment to be not quite similar, and there is no the electrophoresis translational speed that any method can accurate Calculation charged molecule 111 at present.If the biological sample that carries out electrophoresis experiment was for testing in the past, then can calculate that biological sample finishes the required time of electrophoresis according to past experience, but, if biological sample to be tested was not tested before being, then must utilize experimental mistake method (trial ﹠amp; Error) repeatedly attempt the electrophoresis required time, that is after colloid 11 is finished electrophoresis each time, the experimenter must utilize light supply apparatus to confirm whether the result of electrophoresis meets the requirements, if result badly, then must readjust electrophoresis time for colloid 11, come to avoid the electrophoresis time deficiency can't make the charged molecule 111 of different molecular weight in the colloid 11 separate, or the charged molecule 111 of electrophoresis time different molecular weight in causing colloid 11 is for a long time run out of and is fallen into the electrophoresis liquid 103 from colloid 11 totally.
In view of this, the present invention proposes a kind of real-time fluorescence electrophoretic apparatus, can observe the fluorescence performance of biological sample in electrophoresis process, learn the progress of electrophoresis immediately, whether the decision electrophoresis experiment stops, and uses the mistake of avoiding electrophoresis experiment, will be the target that the present invention desires to reach.
Summary of the invention
Fundamental purpose of the present invention is to provide a kind of real-time fluorescence electrophoretic apparatus, and the experimenter can observe the fluorescence performance of biological sample in electrophoresis process, learns the progress of electrophoresis immediately, and whether the decision electrophoresis experiment stops, and uses the mistake of avoiding electrophoresis experiment.
Secondary objective of the present invention, be to provide a kind of real-time fluorescence electrophoretic apparatus, on the surface of optical filter or side, set up at least one antifog processing components, when electrophoresis tank carries out the electrophoresis experiment of biological sample, utilize the demist effect of antifog processing components, the water vapor that can avoid the electrophoresis tank electrophoresis to produce condenses into fog on optical filter, and has influence on the fluorescence performance that the experimenter watches biological sample.
Another purpose of the present invention, be to provide a kind of real-time fluorescence electrophoretic apparatus, it is designed one and has the structure of air-flow guiding, in the mode of utilizing the air-flow guiding water vapor that the electrophoresis tank electrophoresis produces is taken out of, the water vapor of avoiding the electrophoresis tank electrophoresis to produce condenses into fog on optical filter, and has influence on the fluorescence performance that the experimenter watches biological sample.
For this reason, for reaching above-mentioned purpose, the invention provides a kind of real-time fluorescence electrophoretic apparatus, its structure includes: an electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, carry a colloid with biological sample on the platform, and colloid includes the charged molecule of a plurality of biological samples, and colloid, platform, positive electrode and negative electrode are soaked in the electrophoresis liquid; One lid, it is covered by the electrophoresis tank top, includes an optical filter and at least one luminescence component, and optical filter is located at the extended position place, top of colloid, luminescence component is located at least one side of optical filter and in order to the irradiation colloid, is inspired fluorescence to make the biological sample in the colloid; And a power supply unit, electrically connect positive electrode, negative electrode and luminescence component, so that produce electric field between positive electrode and negative electrode, and make charged molecule in colloid, move, and provide luminescence component luminous required power supply.
The present invention also provides a kind of real-time fluorescence electrophoretic apparatus, its structure includes: an electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, platform is a printing opacity platform, carry a colloid with biological sample on the platform, colloid includes the charged molecule of a plurality of biological samples, and at least one luminescence component is arranged in the inner space of platform, luminescence component is in order to the colloid on the irradiation platform, making the biological sample in the colloid inspire fluorescence, and colloid, platform, positive electrode and negative electrode are soaked in the electrophoresis liquid; One lid, it is covered by the electrophoresis tank top, includes an optical filter, and optical filter is located at the extended position place, top of colloid; And a power supply unit, electrically connect positive electrode, negative electrode and luminescence component, so that produce electric field between positive electrode and negative electrode, and make charged molecule in colloid, move, and provide luminescence component luminous required power supply.
The present invention provides a kind of real-time fluorescence electrophoretic apparatus again, its structure includes: an electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, platform is a printing opacity platform, carry a colloid with biological sample on the platform, colloid includes the charged molecule of a plurality of biological samples, and at least one luminescence component is arranged in the inner space of platform, luminescence component is in order to the colloid on the irradiation platform, making the biological sample in the colloid inspire fluorescence, and colloid, platform, positive electrode and negative electrode are soaked in the electrophoresis liquid; One pedestal, include a pedestal part and a vertical component effect, electrophoresis tank is put on pedestal part, pedestal part is provided with an air intake fan, and the air intake fan has an air inlet, and vertical component effect is provided with a breach, and air inlet to the space between breach is a gas channel, the air intake fan drives an air-flow from air inlet, and air communication is crossed gas channel to overflow from breach; One optical filter, it is located at the extended position place, top of colloid and is fixed on the vertical component effect of pedestal, has a space between optical filter and electrophoresis tank and is provided with an air outlet, and the air-flow that overflows from indentation, there will prolong the space propelling, to blow out from air outlet; And a power supply unit, electrically connect positive electrode, negative electrode, luminescence component and air intake fan, so that produce electric field between positive electrode and negative electrode, and make charged molecule in colloid, move, and the power supply that luminescence component is luminous and the rotation of air intake fan is required is provided.
Beneficial functional of the present invention is: by the present invention, in electrophoresis process, the experimenter can observe the fluorescence performance of biological sample, learns the progress of electrophoresis immediately, and whether decision stops electrophoresis experiment, uses the mistake of avoiding electrophoresis experiment.
Describe the present invention below in conjunction with the drawings and specific embodiments, but not as a limitation of the invention.
Description of drawings
Fig. 1 is the structural representation of known electrophoretic apparatus;
Fig. 2 is the structural representation of real-time fluorescence electrophoretic apparatus one preferred embodiment of the present invention;
Fig. 3 is the partial structure vertical view of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 4 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 5 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 6 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 7 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 8 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Fig. 9 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 10 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 11 is the device stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 12 is the device upset stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 13 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 14 is the device stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 15 is the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 16 is the device stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 17 is the device upset stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention;
Figure 18 is the device stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.
Wherein, Reference numeral
100 electrophoretic apparatuss, 10 electrophoresis tanks
101 platforms, 103 electrophoresis liquid
105 positive electrodes, 107 negative electrodes
11 colloids, 111 charged molecules
13 power supply units, 20 electrophoresis tanks
201 platforms, 202 platforms
203 electrophoresis liquid, 205 positive electrodes
207 negative electrodes, 208 spaces
209 air outlets, 21 colloids
211 charged molecules, 23 power supply units
231 first power supply modules, 233 second source assemblies
30 lids, 31 optical filters
311 connecting portions, 33 luminescence components
35 antifog processing components 361 antifog processing components
363 antifog processing components 50 pedestals
501 pedestal part, 503 vertical component effects
51 air intake fans, 511 air inlets
52 bed hedgehopping cylinders, 53 gas channels
55 breach, 59 air-flows
300 real-time fluorescence electrophoretic apparatuss, 301 real-time fluorescence electrophoretic apparatuss
302 real-time fluorescence electrophoretic apparatuss, 303 real-time fluorescence electrophoretic apparatuss
304 real-time fluorescence electrophoretic apparatuss, 305 real-time fluorescence electrophoretic apparatuss
306 real-time fluorescence electrophoretic apparatuss, 307 real-time fluorescence electrophoretic apparatuss
Embodiment
Below in conjunction with the drawings and specific embodiments technical solution of the present invention being described in detail, further understanding purpose of the present invention, scheme and effect, but is not the restriction as claims protection domain of the present invention.
See also Fig. 2 and Fig. 3, be the structural representation and the partial structure vertical view of real-time fluorescence electrophoretic apparatus one preferred embodiment of the present invention.As shown in the figure, present embodiment real-time fluorescence electrophoretic apparatus 300 includes an electrophoresis tank 20, a lid 30 and a power supply unit 23.
Wherein, electrophoresis tank 20 includes a platform 201, an electrophoresis liquid 203, a positive electrode 205 and a negative electrode 207, carrying one (for example: protein, DNA, RNA, polysaccharide has biological sample on the platform 201 ... or the like) colloid 21, colloid 21 includes the charged molecule 211 of a plurality of biological samples, and through stain dyeing, colloid 21, platform 201, positive electrode 205 and negative electrode 207 are soaked in the electrophoresis liquid 203 biological sample.Lid 30 is covered by electrophoresis tank 20 tops and is set up in the place, periphery of electrophoresis tank, it includes an optical filter 31 and at least one luminescence component 33, optical filter 31 is the extended position place, top that an amber optical filter (amber filter) is located at colloid 21, and on the oblique at least one side that is arranged at optical filter 31 of luminescence component 33, down shine colloid 21, its range of exposures can contain whole colloid 21, inspires fluorescence to make the biological sample in the colloid 21.And power supply unit 23 can be a direct current power supply, and it electrically connects positive electrode 205, negative electrode 207 and luminescence component 33, to supply power to positive electrode 205, negative electrode 207 and luminescence component 33.
In electrophoresis experiment, power supply unit 23 is powered, 207 of positive electrode 205 and negative electrodes can produce an electric field, electric field can drive charged molecule 211 in the colloid 21 respectively towards moving with its electrically opposite electrode 205/207, for example: when charged molecule 211 is negative charge, charged molecule 211 can move toward positive electrode 205, and charged molecule 211 is when be positive charge, and then charged molecule 211 past negative electrode 207 directions move.The same time, luminescence component 33 luminous irradiation colloids 21 are with charged molecule 211 fluorescence excitations that make biological sample in the colloid 21.Moreover when the experimenter saw through optical filter 31 and observes the fluorescence reaction of charged molecule 211 of biological samples, optical filter 31 can carry out luminescence component 33 luminous light source wavelength the fluorescence that filtering only allows biological sample and inspired and pass through.
As above implement according to this, but the mobile variation of the charged molecule 211 of experimenter's Real Time Observation biological sample in colloid 21, use the progress of learning electrophoresis, afterwards, the experimenter is according to each position of charged molecule 211 in colloid 21, whether decision stops electrophoresis experiment, not enough or of a specified duration excessively to avoid electrophoresis time, causes the mistake of electrophoresis experiment.
Moreover when electrophoresis experiment, electrophoresis liquid 203 can be subjected to electric field driven and heat up, and produces the water vapor volatilization, and makes and condense into fog on the optical filter 31.Condensing has the optical filter of fog 31 will hinder the fluorescence performance that the experimenter observes biological sample, therefore, the present invention (for example: lower surface) further have additional at least one antifog processing components 35 goes up in a wherein surface of optical filter 31, antifog processing components 35 includes at least one thermal resistance wire, and power supply unit 23 electrically connects antifog processing components 35, to supply power to antifog processing components 35.When antifog processing components 35 is switched on, antifog processing components 35 will be warming up to a specified temp gradually, so that optical filter 31 has a temperature that is higher than room temperature, then the water vapor of electrophoresis liquid is not easy to the fog that freeze-outs on the optical filter 31, and has influence on the fluorescence performance that the experimenter watches biological sample.
See also Fig. 4, be the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.As shown in the figure, the electrophoresis tank 20 of present embodiment real-time fluorescence electrophoretic apparatus 301 and lid 30 its both inner required working powers of electronic package that are provided with can be provided by different power supply modules, power supply unit 23 includes one first power supply module 231 and a second source assembly 233, first power supply module 231 is in order to supply power to positive electrode 205 and negative electrode 207, and second source assembly 233 is in order to supply power to luminescence component 33 and antifog processing components 35, and second source assembly 233 is a power-supply control unit, and whether may command luminescence component 33 is lighted and reached antifog processing components 35 and whether produce heat energy.
See also Fig. 5, be the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.As above shown in Figure 2, the lid 30 of real-time fluorescence electrophoretic apparatus 300 of the present invention removes the place, periphery that can be set up in electrophoresis tank 20, also can be shown in present embodiment real-time fluorescence electrophoretic apparatus 302, and lid 30 directly is fixedly arranged on the electrophoresis tank 20.
See also Fig. 6, be the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.The luminescence component 33 of the various embodiments described above real-time fluorescence electrophoretic apparatus 300/301/302 all is arranged at the side of the optical filter 31 of lid 30, so, shown in present embodiment real-time fluorescence electrophoretic apparatus 303, luminescence component 33 also can change in platform 202 inner spaces of being located at electrophoresis tank 20.
The described platform 202 of present embodiment is a printing opacity platform, carries a colloid 21 with biological sample on the platform 202.Obliquely on the sidewall of the inner space of platform 202 be provided with at least one luminescence component 33, in order to up to shine the colloid 21 on the platform 202, to make the biological sample in the colloid 21 inspire fluorescence, then the experimenter can see through the mobile variation of charged molecule 211 in colloid 21 that optical filter 31 is observed biological sample, learn the progress of electrophoresis immediately, similarly can reach the purpose of the fluorescence performance of Real Time Observation biological sample.Moreover other modular construction and the described content of Fig. 2 embodiment of present embodiment are roughly the same, at this, are not repeating elaboration.
See also Fig. 7, be the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.As above shown in Figure 6, the lid 30 of real-time fluorescence electrophoretic apparatus 303 is set up in the place, periphery of electrophoresis tank 20, and the lid 30 shown in the present embodiment real-time fluorescence electrophoretic apparatus 304 also can be selected to be fixedly arranged on the electrophoresis tank 20.
See also Fig. 8, be the structural representation of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.Compared to the real-time fluorescence electrophoretic apparatus 304 of Fig. 7 embodiment, on a wherein surface of optical filter 31, thermal resistance wire is set as antifog processing components 35, the real-time fluorescence electrophoretic apparatus 305 of present embodiment also can select for use an air-out fan as antifog processing components 361.Antifog processing components 361 is arranged on the wherein side of optical filter 31, extracts out with the water vapor that electrophoresis tank 20 electrophoresis are produced, and avoids being condensed on the optical filter 31.
Perhaps, as shown in Figure 9, also can further set up another antifog processing components 363 in another side of optical filter 31, this antifog processing components 363 is an air intake fan, in order to outside air is blown in this real-time fluorescence electrophoretic apparatus 305, utilize the setting of a pair of air intake/air-out fan, can reach better air-flow guiding in the inside of real-time fluorescence electrophoretic apparatus 305, so that water vapor is detached out from real-time fluorescence electrophoretic apparatus 305 inside up hill and dale, and can't on optical filter 31, condense into fog.
Again, utilize the mode of air-flow guiding that the water vapor that electrophoresis tank 20 electrophoresis produce is taken out of, the present invention proposes different apparatus structures in addition, see also Figure 10, Figure 11 and shown in Figure 12, it is respectively structural representation, device stereographic map and the device upset stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.Present embodiment real-time fluorescence electrophoretic apparatus 306 includes an electrophoresis tank 20, a pedestal 50 and a power supply unit 23.
Wherein, electrophoresis tank 20 includes a platform 202, an electrophoresis liquid 203, a positive electrode 205 and a negative electrode 207.Platform 202 is a printing opacity platform, its carrying one (for example: protein, DNA, RNA, polysaccharide has biological sample ... or the like) colloid 21, colloid 21 includes the charged molecule 211 of a plurality of biological samples, and through stain dyeing, colloid 21, platform 202, positive electrode 205 and negative electrode 207 are soaked in the electrophoresis liquid 203 biological sample.In addition, obliquely on the sidewall of the inner space of platform 202 be provided with at least one luminescence component 33,, inspire fluorescence to make the biological sample in the colloid 21 in order to up to shine the colloid 21 on the platform 202.
When electrophoresis experiment, the electric field driven that the electrophoresis liquid 203 of electrophoresis tank 20 can be subjected to 207 of positive electrode 205 and negative electrodes heats up, and produces the water vapor volatilization, and is attached on the optical filter 31.At this moment, air intake fan 51 drives an air-flow 59 from air inlet 511, air-flow 59 by gas channel 53 to overflow from breach 55, the air-flow 59 that overflows from breach 55 will prolong space 208 propellings, then the water vapor on the optical filter 31 will blow out from air outlet 209 along with air-flow 59, to avoid water vapor on optical filter 31, to condense into fog, watch the fluorescence of biological sample to show and have influence on the experimenter.
The air inlet 511 of present embodiment is positioned on the bottom surface of pedestal part 501, and sets up a plurality of bed hedgehopping cylinders 52 under the bottom surface of pedestal part 501.Utilize the setting of bed hedgehopping cylinder 52, when real-time fluorescence electrophoretic apparatus 306 is put in a plane, will make air inlet 511 and interplanar have more space, help cross-ventilation, can successfully bring air-flow 59 into from air inlet 511 to make air intake fan 51.
Certainly, as shown in figure 13, the air inlet 511 of real-time fluorescence electrophoretic apparatus 306 also can change on the side of being located at pedestal part 501, to save the setting of bed hedgehopping cylinder 52.
Again, as shown in figure 14, in order to increase the propelling intensity of air-flow 59, real-time fluorescence electrophoretic apparatus 306 can be set up pair of side plates 57 on the both sides of pedestal 50, then the air-flow 59 that overflows from breach 55 will can not dissipate from pedestal 50 both sides and cause intensity to weaken, like this, keep certain air-flow 59 intensity so that the water vapor on the optical filter 31 is blown out up hill and dale outside air outlet 209.
See also Figure 15, Figure 16 and Figure 17, be structural representation, device stereographic map and the device upset stereographic map of the another embodiment of real-time fluorescence electrophoretic apparatus of the present invention.As mentioned above, the optical filter 31 of the real-time fluorescence electrophoretic apparatus 306 of a last embodiment is to be fixedly arranged on the vertical component effect 503 of pedestal 50 by a junction 311, so, the optical filter 31 of present embodiment real-time fluorescence electrophoretic apparatus 307 also can be arranged on the frame 70, is fixedly arranged on the vertical component effect 503 of pedestal 50 by frame 70 again.
Moreover the frame 70 of present embodiment also can select identical material to make with pedestal 50, and then both can obtain preferable degree of adhesion when engaging, and firmly be set up on the pedestal 50 to make optical filter 31, reduce the doubt of dropping.
Certainly, as shown in figure 18, real-time fluorescence electrophoretic apparatus 307 also can be set up pair of side plates 57 on the both sides of pedestal 50, to keep certain air-flow 59 intensity the water vapor on the optical filter 31 is blown out up hill and dale outside air outlet 209.
The above, it only is a preferred embodiment of the present invention, be not to be used for limiting scope of the invention process, promptly all equalizations of doing according to the described shape of the present patent application claim, structure, feature and spirit change and modify, and all should be included in the claim of the present invention.
Claims (12)
1. a real-time fluorescence electrophoretic apparatus is characterized in that, its structure includes:
One electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, carry a colloid with biological sample on this platform, and this colloid includes the charged molecule of a plurality of biological samples, and this colloid, this platform, this positive electrode and this negative electrode are soaked in this electrophoresis liquid;
One lid, it is covered by this electrophoresis tank top, include an optical filter and at least one luminescence component, this optical filter is located at the extended position place, top of this colloid, this luminescence component is located at least one side of this optical filter and in order to shine this colloid, inspires fluorescence to make the biological sample in this colloid; And
One power supply unit electrically connects this positive electrode, this negative electrode and this luminescence component, so that produce electric field between this positive electrode and this negative electrode, and makes this charged molecule move in this colloid, and provides this luminescence component luminous required power supply.
2. real-time fluorescence electrophoretic apparatus as claimed in claim 1 is characterized in that, this lid is set up in the place, periphery of this electrophoresis tank or is fixedly arranged on the cell body of this electrophoresis tank.
3. real-time fluorescence electrophoretic apparatus as claimed in claim 1, it is characterized in that, this lid includes at least one antifog processing components, it is arranged on the wherein surface of this optical filter of this lid, in order to produce fog on the surface that prevents this optical filter, and this power supply unit electrically connects this antifog processing components, to supply power to this antifog processing components.
4. real-time fluorescence electrophoretic apparatus as claimed in claim 3 is characterized in that, this antifog processing components includes at least one thermal resistance wire, in order to produce heat energy on this optical filter.
5. a real-time fluorescence electrophoretic apparatus is characterized in that, its structure includes:
One electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, this platform is a printing opacity platform, carry a colloid with biological sample on this platform, this colloid includes the charged molecule of a plurality of biological samples, and at least one luminescence component is arranged in the inner space of this platform, this luminescence component is in order to shine this colloid on this platform, making the biological sample in this colloid inspire fluorescence, and this colloid, this platform, this positive electrode and this negative electrode are soaked in this electrophoresis liquid;
One lid, it is covered by this electrophoresis tank top, includes an optical filter, and this optical filter is located at the extended position place, top of this colloid; And
One power supply unit electrically connects this positive electrode, this negative electrode and this luminescence component, so that produce electric field between this positive electrode and this negative electrode, and makes this charged molecule move in this colloid, and provides this luminescence component luminous required power supply.
6. real-time fluorescence electrophoretic apparatus as claimed in claim 5 is characterized in that, this lid is set up in the place, periphery of this electrophoresis tank or is fixedly arranged on the cell body of this electrophoresis tank.
7. real-time fluorescence electrophoretic apparatus as claimed in claim 5, it is characterized in that, this lid includes at least one antifog processing components, its wherein surface that is arranged at this optical filter of this lid go up or at least one side of this optical filter on, in order to produce fog on the surface that prevents this optical filter, and this power supply unit electrically connects this antifog processing components, to supply power to this antifog processing components.
8. real-time fluorescence electrophoretic apparatus as claimed in claim 7, it is characterized in that, this antifog processing components includes at least one thermal resistance wire, an air-out fan or an air intake fan, this thermal resistance wire is arranged on the wherein surface of this optical filter, and this air-out fan or this air intake fan then are located on the wherein side of this optical filter.
9. a real-time fluorescence electrophoretic apparatus is characterized in that, its structure includes:
One electrophoresis tank, include a platform, an electrophoresis liquid, a positive electrode and a negative electrode, this platform is a printing opacity platform, carry a colloid with biological sample on this platform, this colloid includes the charged molecule of a plurality of biological samples, and at least one luminescence component is arranged in the inner space of this platform, this luminescence component is in order to shine this colloid on this platform, making the biological sample in this colloid inspire fluorescence, and this colloid, this platform, this positive electrode and this negative electrode are soaked in this electrophoresis liquid;
One pedestal, include a pedestal part and a vertical component effect, this electrophoresis tank is put on this pedestal part, this pedestal part is provided with an air intake fan, and this air intake fan has an air inlet, and this vertical component effect is provided with a breach, and this air inlet to the space between this breach is a gas channel, this air intake fan drives an air-flow from this air inlet, and this air communication is crossed this gas channel to overflow from this breach;
One optical filter, it is located at the extended position place, top of this colloid and is fixed on this vertical component effect of this pedestal, have a space between this optical filter and this electrophoresis tank and be provided with an air outlet, this air-flow that overflows from this indentation, there will prolong this space propelling, to blow out from this air outlet; And
One power supply unit, electrically connect this positive electrode, this negative electrode, this luminescence component and this air intake fan, so that produce electric field between this positive electrode and this negative electrode, and make this charged molecule in this colloid, move, and provide this luminescence component the luminous and required power supply of this air intake fan rotation.
10. real-time fluorescence electrophoretic apparatus as claimed in claim 9 is characterized in that, this air inlet is positioned on the bottom surface or side of this pedestal part.
11. real-time fluorescence electrophoretic apparatus as claimed in claim 9 is characterized in that, this optical filter is fixed on this vertical component effect of this pedestal by a junction.
12. real-time fluorescence electrophoretic apparatus as claimed in claim 9 is characterized in that, this optical filter is arranged in the frame, to be fixed on this vertical component effect of this pedestal by this frame.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107340327A (en) * | 2017-07-24 | 2017-11-10 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
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| ES2144374B1 (en) * | 1998-07-01 | 2001-01-01 | Univ Barcelona Autonoma | TRANSILUMINATOR FOR THE VISUALIZATION OF FLUORESCENT PROTEIN AND DNA BANDS IN ELECTROPHORETIC GELS. |
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| CN2646709Y (en) * | 2003-08-26 | 2004-10-06 | 厦门百维信生物科技有限公司 | Equipment for real-time observing gel electrophoresis |
| CN1766581A (en) * | 2005-11-04 | 2006-05-03 | 深圳大学 | Safety nucleic acid electrophoresis fluorimeter |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107340327A (en) * | 2017-07-24 | 2017-11-10 | 西工大常熟研究院有限公司 | A kind of gel electrophoresis groove with LED light source |
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