CN215493308U - DNA electrophoresis rubber running device - Google Patents
DNA electrophoresis rubber running device Download PDFInfo
- Publication number
- CN215493308U CN215493308U CN202121673188.8U CN202121673188U CN215493308U CN 215493308 U CN215493308 U CN 215493308U CN 202121673188 U CN202121673188 U CN 202121673188U CN 215493308 U CN215493308 U CN 215493308U
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- heat dissipation
- box body
- side wall
- gel
- vertical baffles
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 36
- 230000017525 heat dissipation Effects 0.000 claims abstract description 52
- 239000000499 gel Substances 0.000 claims abstract description 36
- 239000007853 buffer solution Substances 0.000 claims abstract description 22
- 239000011543 agarose gel Substances 0.000 claims abstract description 15
- 238000005057 refrigeration Methods 0.000 claims description 19
- 239000004065 semiconductor Substances 0.000 claims description 15
- 239000011521 glass Substances 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 3
- 229920000715 Mucilage Polymers 0.000 claims 1
- 239000000853 adhesive Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 238000004925 denaturation Methods 0.000 abstract description 5
- 230000036425 denaturation Effects 0.000 abstract description 5
- 239000003292 glue Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 8
- 238000001816 cooling Methods 0.000 description 5
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a DNA electrophoresis glue running device, which relates to the technical field of electrophoresis equipment and has the technical scheme key points that: the box body is internally provided with a heat dissipation device and two vertical baffles at the bottom end, and the heat dissipation device is positioned between the two vertical baffles; a gel supporting plate is arranged between the two vertical baffles, agarose gel is arranged at the top of the gel supporting plate, and buffer solution is arranged among the agarose gel, the two vertical baffles and the inner wall of the box body; a refrigerating device is embedded in each of the two vertical baffles; the lateral wall of box is equipped with the U-shaped support, and the lateral wall fixed connection of the inside wall of the vertical section of U-shaped support and box, the horizontal segment of U-shaped support are located the box directly over, and the bottom of the horizontal segment of U-shaped support is equipped with the ultraviolet fluorescent tube. The device can maintain the temperature of the buffer solution at a proper temperature in the electrophoresis process to prevent DNA denaturation; meanwhile, the device can directly irradiate the gel through an ultraviolet lamp to observe the position of the DNA.
Description
Technical Field
The utility model relates to the technical field of electrophoresis equipment, in particular to a DNA electrophoresis gel running device.
Background
Electrophoresis refers to a phenomenon in which charged particles or molecules move in an electric field, and a technique for separating substances by utilizing the electrophoresis is called electrophoresis. Many biological macromolecules such as nucleic acid can be separated by electrophoresis technology, which is an indispensable important analysis means for molecular biology research.
After the DNA molecules are extracted, the quantity and quality of the DNA molecules need to be detected by electrophoresis. Since the introduction of agarose and polyacrylamide gels into nucleic acid research, gel electrophoresis techniques for separating DNA by relative molecular mass have evolved as an important experimental means for the analytical identification of DNA molecules.
The prior DNA electrophoresis gel running device has the following technical defects:
1. during electrophoresis, the temperature of the buffer solution rises, and DNA is denatured due to too high temperature;
2. after the existing DNA electrophoresis is finished, the agarose gel needs to be taken out, and then the ultraviolet lamp is used for irradiating the gel to observe the position of the DNA, so that the operation is inconvenient.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a DNA electrophoresis gel running device which can maintain the temperature of a buffer solution at a proper temperature in the electrophoresis process and prevent DNA from denaturation; meanwhile, the device can directly irradiate the gel through an ultraviolet lamp to observe the position of the DNA.
The technical purpose of the utility model is realized by the following technical scheme: a DNA electrophoresis glue running device comprises a box body, wherein the bottom end inside the box body is provided with a heat dissipation device and two vertical baffles, and the heat dissipation device is positioned between the two vertical baffles; a gel supporting plate is arranged between the two vertical baffles, the two vertical baffles are respectively and fixedly connected with two opposite side walls of the gel supporting plate, the gel supporting plate is positioned right above the heat dissipation device, agarose gel is arranged at the top of the gel supporting plate, and buffer solution is arranged among the agarose gel, the two vertical baffles and the inner wall of the box body; a refrigerating device is embedded in each of the two vertical baffles; the outer side wall of the box body is provided with a U-shaped bracket, the inner side wall of the vertical section of the U-shaped bracket is fixedly connected with the outer side wall of the box body, the horizontal section of the U-shaped bracket is positioned right above the box body, and the bottom of the horizontal section of the U-shaped bracket is provided with an ultraviolet lamp tube; the lateral wall of box is equipped with display control panel, control processor and power cord jack.
By adopting the technical scheme, the refrigerating device is embedded in the vertical baffle, so that the buffer solution can be cooled by the refrigerating device, and DNA denaturation caused by overhigh buffer solution in the electrophoresis gel running process is prevented; the heat dissipation device is arranged right below the gel supporting plate, so that heat generated by the refrigerating device can be discharged out of the device, and the refrigerating effect is enhanced; the ultraviolet lamp tube is arranged at the bottom of the horizontal section of the U-shaped bracket, so that the DNA position can be observed directly through ultraviolet lamp irradiation, and the effect of convenient observation is achieved.
The utility model is further configured to: the refrigerating device comprises a plurality of radiating fins, a second radiating plate, a plurality of refrigerating semiconductors and a first radiating plate; the refrigeration ends of the refrigeration semiconductors are fixedly connected with the side wall of the first heat dissipation plate, and the side wall of the first heat dissipation plate, far away from the refrigeration semiconductors, is fixedly connected with the inner side wall of the vertical baffle, close to the buffer solution; and the heating end of the refrigeration semiconductor is fixedly connected with the side wall of the second heat dissipation plate, and the end parts of the plurality of heat dissipation fins are fixedly connected with the side wall of the second heat dissipation plate.
Through adopting above-mentioned technical scheme, refrigerating plant adopts the semiconductor refrigeration, and the noise is little, and refrigeration effect is good, can reduce the temperature of buffer solution when the electrophoresis.
The utility model is further configured to: the heat dissipation device comprises a motor base, a fan and a rotating motor, the bottom of the motor base is fixedly connected with the top end of the inner portion of the box, the rotating motor is fixedly connected with the top of the motor base, and the output end of the rotating motor is connected with the fan.
By adopting the technical scheme, the heat dissipation device adopts the rotating motor to drive the fan to rotate, so that the temperature of the heat dissipation end of the refrigeration device can be rapidly reduced, and the optimal refrigeration effect is achieved.
The utility model is further configured to: the novel box is characterized in that sliding grooves are symmetrically formed in the inner side wall of the box body, transparent glass covers are arranged in the sliding grooves, a handle is arranged at one end of each transparent glass cover, and the other end of each transparent glass cover penetrates through one side wall of the box body to be clamped with the other side wall of the box body.
Through adopting above-mentioned technical scheme, with transparent glass lid joint in the spout, in the electrophoresis process, can cover the top of box, prevent that partial buffer solution from ejecting from the box when the electrolysis.
The utility model is further configured to: the top of the two opposite side walls of the box body is respectively provided with a first electrode and a second electrode, and the first electrode and the second electrode are respectively positioned on two sides of the two vertical baffles.
By adopting the technical scheme, the electrophoresis of the DNA can be realized by arranging the first electrode and the second electrode at the top of the box body.
The utility model is further configured to: the side wall of the box body close to the heat dissipation device is provided with a plurality of heat dissipation holes.
By adopting the technical scheme, the side wall of the box body close to the heat dissipation device is provided with the plurality of heat dissipation holes, so that heat generated by the heat dissipation fins can be dissipated outside the device.
In conclusion, the utility model has the following beneficial effects: the refrigerating device is embedded in the vertical baffle, so that the buffer solution can be cooled through the refrigerating device, and DNA denaturation caused by overhigh buffer solution in the electrophoresis gel running process is prevented; the heat dissipation device is arranged right below the gel supporting plate, so that heat generated by the refrigerating device can be discharged out of the device, and the refrigerating effect is enhanced; the ultraviolet lamp tube is arranged at the bottom of the horizontal section of the U-shaped bracket, so that the DNA position can be observed directly through ultraviolet lamp irradiation, and the effect of convenient observation is achieved.
Drawings
FIG. 1 is a right sectional view of a DNA electrophoresis gel running device according to an embodiment of the present invention;
FIG. 2 is a schematic perspective view of a DNA electrophoresis gel running device according to an embodiment of the present invention;
FIG. 3 is a top view of a DNA electrophoresis gel running device according to an embodiment of the present invention.
In the figure: 1. an ultraviolet lamp tube; 2. a first electrode; 3. a U-shaped bracket; 4. a transparent glass cover; 5. a vertical baffle; 6. a buffer solution; 7. a box body; 8. a first heat dissipation plate; 9. a refrigeration semiconductor; 10. a second heat dissipation plate; 11. a fan; 12. a motor base; 13. a rotating electric machine; 14. a heat sink; 15. a gel tray; 16. agarose gel; 17. a chute; 18. a display control panel; 19. a control processor; 20. a power line jack; 21. heat dissipation holes; 22. a handle; 23. a second electrode.
Detailed Description
The present invention is described in further detail below with reference to figures 1-3.
Example (b): a DNA electrophoresis glue running device is shown in figures 1 to 3 and comprises a box body 7, wherein a heat dissipation device and two vertical baffles 5 are fixedly arranged at the bottom end inside the box body 7, and the heat dissipation device is positioned between the two vertical baffles 5; a gel supporting plate 15 is fixedly arranged between the two vertical baffle plates 5, the two vertical baffle plates 5 are respectively and fixedly connected with two opposite side walls of the gel supporting plate 15, the gel supporting plate 15 is positioned right above the heat dissipation device, agarose gel 16 is placed at the top of the gel supporting plate 15, and buffer solution 6 is poured among the agarose gel 16, the two vertical baffle plates 5 and the inner wall of the box body 7; a refrigerating device is embedded in each of the two vertical baffles 5; the outer side wall of the box body 7 is fixedly provided with a U-shaped support 3, the inner side wall of the vertical section of the U-shaped support 3 is fixedly connected with the outer side wall of the box body 7, the horizontal section of the U-shaped support 3 is positioned right above the box body 7, and the bottom of the horizontal section of the U-shaped support 3 is fixedly provided with an ultraviolet lamp tube 1; the outer side wall of the box body 7 is fixedly provided with a display control panel 18, a control processor 19 and a power line jack 20.
In this embodiment, when the operator needs to use the running device, the agarose gel 16 is first placed on the gel tray 15, the labeled DNA of the nucleic acid fluorescent dye is placed on the agarose gel 16, and the buffer solution is poured into the device so that the buffer solution is over the agarose gel 16; after the preparation work is done, the power line jack 20 is inserted into a power line to be electrified, then a switch button on the display control panel 18 is clicked, at the moment, the control processor 19 controls the first electrode 2 and the second electrode 23 to be electrified, meanwhile, the control processor 19 controls the refrigeration device and the heat dissipation device to start running, and the ultraviolet lamp tube 1 is also lighted; after electrophoresis is finished, the power is turned off, and the operator can directly see the position of the DNA in the agarose gel 16 through the irradiation of the ultraviolet lamp 1.
The refrigerating device comprises a plurality of radiating fins 14, a second radiating plate 10, a plurality of refrigerating semiconductors 9 and a first radiating plate 8; the refrigeration ends of the refrigeration semiconductors 9 are fixedly connected with the side wall of the first heat dissipation plate 8, and the side wall of the first heat dissipation plate 8 far away from the refrigeration semiconductors 9 is fixedly connected with the inner side wall of the vertical baffle 5 close to the buffer solution 6; the heating end of the refrigeration semiconductor 9 is fixedly connected with the side wall of the second heat dissipation plate 10, and the end parts of the plurality of heat dissipation fins 14 are fixedly connected with the side wall of the second heat dissipation plate 10.
In this embodiment, after the device is powered on, the power supply is used for conducting forward power on the refrigeration semiconductor 9, so that the first cooling plate 8 generates cooling capacity, the second cooling plate 10 generates heat, and the cooling capacity on the first cooling plate 8 keeps the temperature of the buffer solution 6 at a proper temperature through heat conduction and convection heat exchange; meanwhile, the heat on the second heat dissipation plate 10 is transferred to the heat dissipation plate 14 through heat conduction, and the heat dissipation plate 14 releases the heat into the device through convection heat exchange.
The heat dissipation device comprises a motor base 12, a fan 11 and a rotating motor 13, the bottom of the motor base 12 is fixedly connected with the top end inside the box 7, the rotating motor 13 is fixedly connected with the top of the motor base 12, and the output end of the rotating motor 13 is connected with the fan 11.
In this embodiment, after the device is powered on, the control processor 19 controls the rotating motor 13 to rotate, and the rotating motor 13 drives the fan 11 to rotate, so that the heat discharged by the heat sink 14 through convection heat exchange is quickly discharged out of the device.
The inside wall symmetry of box 7 is equipped with spout 17, installs transparent glass lid 4 in the spout 17, and the one end fixed mounting of transparent glass lid 4 has handle 22, and the other end of transparent glass lid 4 passes a lateral wall of box 7 and another lateral wall joint of box 7.
In this embodiment, during electrophoresis, an operator holds the handle 22 to move the transparent glass cover 4 along the slide slot 17 on the side wall of the box 7, so as to cover the top of the box 7, and after completing the spotting, pulls the transparent glass cover 4 in the opposite direction, and then observes the position of the DNA by irradiation of the ultraviolet lamp 1.
The top of two opposite side walls of the box 7 is respectively fixedly provided with a first electrode 2 and a second electrode 23, and the first electrode 2 and the second electrode 23 are respectively positioned at two sides of the two vertical baffles 5.
In this embodiment, the first electrode 2 and the second electrode 23 are electrified, and the electrodes are positive and negative electrodes, respectively, so that the DNA can move on the agarose gel 16.
The side wall of the box body 7 close to the heat dissipation device is provided with a plurality of heat dissipation holes 21.
In the present embodiment, the heat dissipated by the heat dissipation fins 14 is discharged out of the device through the heat dissipation holes 21, so that the refrigeration device can operate normally.
The working principle is as follows: by embedding the refrigerating device in the vertical baffle 5, the buffer solution 6 can be cooled by the refrigerating device, so that DNA denaturation caused by overhigh buffer solution 6 in the electrophoresis glue running process is prevented; the heat dissipation device is arranged right below the gel supporting plate 15, so that heat generated by the refrigerating device can be discharged out of the device, and the refrigerating effect is enhanced; through installing ultraviolet fluorescent tube 1 in the horizontal segment bottom of U-shaped support 3, can directly observe the DNA position through ultraviolet lamp irradiation, reach the effect of being convenient for observe.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (6)
1. The utility model provides a DNA electrophoresis runs mucilage binding and puts, includes box (7), characterized by: the bottom end in the box body (7) is provided with a heat dissipation device and two vertical baffles (5), and the heat dissipation device is positioned between the two vertical baffles (5); a gel supporting plate (15) is arranged between the two vertical baffles (5), the two vertical baffles (5) are respectively and fixedly connected with two opposite side walls of the gel supporting plate (15), the gel supporting plate (15) is positioned right above the heat dissipation device, agarose gel (16) is arranged at the top of the gel supporting plate (15), and buffer solution (6) is arranged among the agarose gel (16), the two vertical baffles (5) and the inner wall of the box body (7); refrigerating devices are embedded in the two vertical baffles (5);
the outer side wall of the box body (7) is provided with a U-shaped support (3), the inner side wall of the vertical section of the U-shaped support (3) is fixedly connected with the outer side wall of the box body (7), the horizontal section of the U-shaped support (3) is positioned right above the box body (7), and the bottom of the horizontal section of the U-shaped support (3) is provided with an ultraviolet lamp tube (1); the outer side wall of the box body (7) is provided with a display control panel (18), a control processor (19) and a power line jack (20).
2. The DNA electrophoresis gel running device of claim 1, wherein: the refrigerating device comprises a plurality of radiating fins (14), a second radiating plate (10), a plurality of refrigerating semiconductors (9) and a first radiating plate (8); the refrigeration ends of the refrigeration semiconductors (9) are fixedly connected with the side wall of the first heat dissipation plate (8), and the side wall, far away from the refrigeration semiconductors (9), of the first heat dissipation plate (8) is fixedly connected with the inner side wall, close to the buffer solution (6), of the vertical baffle (5); the heating end of the refrigerating semiconductor (9) is fixedly connected with the side wall of the second heat dissipation plate (10), and the end parts of the plurality of heat dissipation fins (14) are fixedly connected with the side wall of the second heat dissipation plate (10).
3. The DNA electrophoresis gel running device of claim 1, wherein: the heat dissipation device comprises a motor base (12), a fan (11) and a rotating motor (13), the bottom of the motor base (12) is fixedly connected with the top end inside the box body (7), the rotating motor (13) is fixedly connected with the top of the motor base (12), and the output end of the rotating motor (13) is connected with the fan (11).
4. The DNA electrophoresis gel running device of claim 1, wherein: the inside wall symmetry of box (7) is equipped with spout (17), be equipped with transparent glass lid (4) in spout (17), the one end of transparent glass lid (4) is equipped with handle (22), the other end of transparent glass lid (4) passes a lateral wall of box (7) and another lateral wall joint of box (7).
5. The DNA electrophoresis gel running device of claim 1, wherein: the top of two opposite side walls of the box body (7) is respectively provided with a first electrode (2) and a second electrode (23), and the first electrode (2) and the second electrode (23) are respectively positioned on two sides of the two vertical baffles (5).
6. The DNA electrophoresis gel running device of claim 1, wherein: the side wall of the box body (7) close to the heat dissipation device is provided with a plurality of heat dissipation holes (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121673188.8U CN215493308U (en) | 2021-07-22 | 2021-07-22 | DNA electrophoresis rubber running device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121673188.8U CN215493308U (en) | 2021-07-22 | 2021-07-22 | DNA electrophoresis rubber running device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215493308U true CN215493308U (en) | 2022-01-11 |
Family
ID=79751093
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121673188.8U Active CN215493308U (en) | 2021-07-22 | 2021-07-22 | DNA electrophoresis rubber running device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215493308U (en) |
-
2021
- 2021-07-22 CN CN202121673188.8U patent/CN215493308U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240326 Address after: Room 601, Unit 2, Building 9, No. 168 Tiyu South Road, Xiaodian District, Taiyuan City, Shanxi Province, 030000 Patentee after: Shanxi Huixin Tonglian Technology Co.,Ltd. Country or region after: China Address before: 046000 No. 161 Jiefang East Street, Changzhi City, Shanxi Province Patentee before: CHANGZHI MEDICAL College Country or region before: China |