CN219032199U - Nucleic acid extractor with heat insulation structure - Google Patents

Abstract

The utility model discloses a nucleic acid extractor with a heat insulation structure, which comprises a shell, wherein the top of the bottom wall of the shell is connected with a decorative plate, the top of the decorative plate is provided with two groups of placing mechanisms which are distributed in parallel from left to right, each group of placing mechanisms comprises a first placing plate connected with the decorative plate, and one side of the first placing plate is provided with a second placing plate connected with the decorative plate. According to the utility model, the heat insulation sleeves are arranged on the outer sides of the first placing plate and the second placing plate, so that heat at the two places is concentrated, the nucleic acid release rate of a sample liquid area is accelerated, and meanwhile, the elution efficiency of a magnetic rod of an eluent area is accelerated, and purified nucleic acid is rapidly obtained; through the first board and the second board interval of placing of every placing mechanism place, make the regional abundant exposure of rinsing liquid in the external world, avoid here heat gathering, lead to the rinsing liquid to volatilize, realize placing mechanism's thermal-insulated heat preservation, accord with nucleic acid extraction process, facilitate the use.

Description

Nucleic acid extractor with heat insulation structure

Technical Field

The utility model particularly relates to a nucleic acid extractor with a heat insulation structure.

Background

Nucleic acid is a biological macromolecular compound polymerized from many nucleotides, and is one of the most basic substances for life. Nucleic acids are widely found in all animal and plant cells and microorganisms, and nucleic acids in organisms are often combined with proteins to form nucleoproteins. The method for extracting the nucleic acid comprises a CTAB (CTAB), a glass bead method, an ultrasonic method, a grinding method, a freeze thawing method, a guanidine isothiocyanate method, a magnetic bead method and the like, wherein the magnetic bead method is used for extracting the nucleic acid through a magnetic rod which is matched with the magnetic bead method, namely the magnetic bead adsorbs the nucleic acid in a sample solution, and the magnetic bead is adsorbed by the magnetic rod, so that the magnetic bead with the nucleic acid is transferred in reagents such as a lysate, a cleaning solution, an eluent and the like, and the purer nucleic acid is finally obtained. When the nucleic acid extraction instrument is in actual use, in order to improve the mixing rate of the sample liquid and the lysis liquid in the sample box, a heating film is generally used for heating the sample seat, so that the temperature in the sample box is improved, and the mixing rate of the sample liquid and the lysis liquid is ensured;

however, when the current nucleic acid extractor is used, the sample seat only has the function of installing the sample box, has no heat insulation function, and the heat of the heating film is easy to diffuse outwards through the sample box and cannot be concentrated in the sample box, so that the mixing rate of the lysate and the sample liquid is low, and the nucleic acid extraction efficiency is low; meanwhile, the rinse liquid is easy to volatilize when being heated greatly, so that the rinse liquid does not need to be heated and insulated, and the importance of designing a sample seat capable of directionally insulating heat is self-evident.

Therefore, it is necessary to invent a nucleic acid extractor having an insulating structure to solve the above-mentioned problems.

Disclosure of Invention

The utility model aims to provide a nucleic acid extractor with a heat insulation structure, wherein heat jackets are arranged on the outer sides of a first placing plate and a second placing plate, so that heat overflow of a sample liquid area and an eluent area is avoided, heat at the two places is concentrated, the release rate of nucleic acid in the sample liquid area is accelerated, and the eluent area is insulated, so that the elution efficiency of a magnetic rod can be accelerated, and purified nucleic acid can be obtained rapidly; further, through the first board and the second board of placing of every placing mechanism place at intervals, make the abundant exposure in the external world in rinsing liquid region, avoid here heat gathering, lead to rinsing liquid to volatilize, realize placing mechanism's thermal-insulated, accord with the nucleic acid extraction process to solve the above-mentioned shortcoming in the technique.

In order to achieve the above object, the present utility model provides the following technical solutions: the nucleic acid extractor with the heat insulation structure comprises a shell, wherein the top of the bottom wall of the shell is connected with a decorative plate, and the top of the decorative plate is provided with two groups of placing mechanisms which are distributed in parallel from left to right;

every group place the mechanism all include the first board of placing that is connected with the decorative board, first board one side of placing is equipped with the second of placing that is connected with the decorative board and places the board, a plurality of slot has all been seted up at first board and the second of placing board top, slot internal connection has the sample box that is used for storing sample liquid, rinsing liquid and eluent, the decorative board bottom is equipped with the heating membrane that is used for heating the sample box, the heating membrane is connected with the casing diapire, first board and the second of placing are placed the board outside and all are equipped with the insulating sheath that is connected with the decorative board for the heat that the interval heating membrane gives off realizes the continuation heat preservation of sample box, the casing rear side is equipped with the extraction mechanism who is used for extracting the inside nucleic acid of sample liquid.

Preferably, the extraction mechanism comprises two first sliding rails which are distributed in parallel from front to back, the sliding rails are connected with the bottom wall of the shell, the top of each sliding rail is connected with a movable support, a ball screw is arranged at the bottom of each movable support in a penetrating mode, a second sliding rail is connected to the movable front side of each movable support, a magnetic rod rack is connected to the top of each second sliding rail, and a magnetic rod sleeve rack is connected to the bottom of each second sliding rail.

Preferably, the sample box is formed by connecting a sample liquid area, a rinsing liquid area and an eluent area from left to right, the sample liquid area is spliced at the top of the first placing plate through a slot, and the eluent is spliced at the top of the second placing plate through the slot.

Preferably, the top slots of each first placing plate are longitudinally distributed from front to back, and the top slots of the second placing plates are distributed in one-to-one correspondence with the tops of the first placing plates.

Preferably, one side of the bottom wall of the shell is connected with a first motor, and the output end of the first motor is connected with a ball screw.

Preferably, the bottom of the movable support is connected with a second motor, the output end of the second motor is in transmission connection with the magnetic rod sleeve frame through a belt, the side wall of the top of the movable support, which is positioned on the second motor, is connected with a third motor, and the output end of the third motor is in transmission connection with the magnetic rod frame through a belt.

Preferably, the bottoms of the left side and the right side of the magnetic rod rack are connected with a plurality of magnetic rods, the bottoms of the left side and the right side of the magnetic rod sleeve rack are connected with a plurality of magnetic rod sleeves, and the number of the magnetic rods is equal to that of the magnetic rod sleeves.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the utility model, the heat insulation sleeves are arranged on the outer sides of the first placing plate and the second placing plate, so that the heat of the sample liquid area and the heat of the eluent area are prevented from overflowing, the heat of the two places are concentrated, the heating effect of the lysate and the sample liquid is further enhanced, the mixing rate of the lysate and the sample liquid is improved, the nucleic acid release rate is accelerated, and the eluent area is insulated, so that the eluting efficiency of a magnetic rod can be accelerated, and purified nucleic acid can be rapidly obtained;

2. according to the utility model, the first placing plate and the second placing plate of each placing mechanism are placed at intervals, so that the rinsing liquid area is positioned between the first placing plate and the second placing plate and is fully exposed to the outside, thereby avoiding heat accumulation at the place to volatilize the rinsing liquid, namely, the heat insulation sleeve only keeps the areas of the first placing plate and the second placing plate of the placing mechanism and keeps the other areas without heat insulation, thereby realizing heat insulation and heat preservation of the placing mechanism, conforming to a nucleic acid extraction procedure, and being convenient for workers to use.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present utility model, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.

FIG. 1 is a perspective view of the present utility model;

FIG. 2 is a schematic view of a placement mechanism according to the present utility model;

FIG. 3 is an exploded view of the sleeve, first placement plate and sample box of the present utility model;

FIG. 4 is a schematic view of the connection structure of the heating film and the decorative plate of the utility model;

FIG. 5 is a schematic diagram of an extraction mechanism according to the present utility model;

fig. 6 is a schematic view of an extraction mechanism according to another aspect of the present utility model.

Reference numerals illustrate:

1 a shell, 2 a decorative plate, 3 a placing mechanism, 31 a first placing plate, 32 a second placing plate, 33 a slot, 34 a sample box, 35 a heating film, 36 a heat insulation sleeve, 4 an extracting mechanism, 41 a first slide rail, 42 a moving bracket, 43 a ball screw, 44 a second slide rail, 45 a magnetic rod rack, 46 a magnetic rod sleeve rack, 341 a sample liquid area, 342 a rinsing liquid area, 343 an eluent area, 411 a first motor, 421 a second motor and 422 a third motor.

Detailed Description

In order to make the technical scheme of the present utility model better understood by those skilled in the art, the present utility model will be further described in detail with reference to the accompanying drawings.

The utility model provides a nucleic acid extractor with a heat insulation structure as shown in figures 1-6, which comprises a shell 1, wherein the top of the bottom wall of the shell 1 is connected with a decorative plate 2, and the top of the decorative plate 2 is provided with two groups of placing mechanisms 3 which are distributed in parallel from left to right;

each group of placing mechanisms 3 comprises a first placing plate 31 connected with a decorative plate 2, a second placing plate 32 connected with the decorative plate 2 is arranged on one side of the first placing plate 31, a plurality of slots 33 are formed in the tops of the first placing plate 31 and the second placing plate 32, sample boxes 34 used for storing sample liquid, rinsing liquid and eluent are connected inside the slots 33, heating films 35 used for heating the sample boxes 34 are arranged at the bottoms of the decorative plate 2, the heating films 35 are connected with the bottom wall of the shell 1, heat jackets 36 connected with the decorative plate 2 are arranged outside the first placing plate 31 and the second placing plate 32 and used for separating heat emitted by the heating films 35, continuous heat preservation of the sample boxes 34 is achieved, an extracting mechanism 4 used for extracting nucleic acid inside the sample liquid is arranged on the rear side of the shell 1, the extracting mechanism 4 comprises two first sliding rails 41 which are distributed from front to back in parallel, the sliding rails are connected with the bottom wall of the shell, moving supports 42 are connected with the tops of the sliding rails, ball screws 43 are arranged at the bottoms of the moving supports 42 in a penetrating mode, sliding rails 44 are connected with second magnetic rails 44, and magnetic bars 44 are connected with the bottoms of the second magnetic supports 44.

Further, in the above technical solution, the sample box 34 is formed by connecting a sample liquid area 341, a rinsing liquid area 342 and an eluent area 343 from left to right, the sample liquid area 341 is inserted into the top of the first placement plate 31 through the slot 33, the eluent is inserted into the top of the second placement plate 32 through the slot 33, each of the slots 33 on the top of the first placement plate 31 is longitudinally distributed from front to back, the slots 33 on the top of the second placement plate 32 are correspondingly distributed with the tops of the first placement plate 31 one by one, the bottoms of the left and right sides of the magnetic bar rack 45 are respectively connected with a plurality of magnetic bars, and the bottoms of the left and right sides of the magnetic bar rack 46 are respectively connected with a plurality of magnetic bar sleeves, and the number of the magnetic bars is equal to the number of the magnetic bar sleeves.

Further, in the above technical solution, a first motor 411 is connected to one side of the bottom wall of the housing 1, an output end of the first motor 411 is connected to the ball screw 43, and provides a power source for the moving bracket 42, a second motor 421 is connected to a bottom of the moving bracket 42, an output end of the second motor 421 is in transmission connection with the magnetic rod sleeve 46 through a belt, and provides a power source for vibration of the magnetic rod sleeve 46, a third motor 422 is connected to a top side wall of the moving bracket 42, which is located on the second motor 421, and an output end of the third motor 422 is in transmission connection with the magnetic rod sleeve 45 through a belt, and provides a power source for up-down movement of the magnetic rod.

The implementation mode specifically comprises the following steps: when the utility model is used, the bracket is positioned on the left side in the shell 1, namely the left magnetic rod and the magnetic rod sleeve are positioned above the left placement mechanism 3, the right magnetic rod and the magnetic rod sleeve are positioned above the right placement mechanism 3, a worker tilts proper amounts of sample liquid, rinsing liquid and eluent in the sample liquid area 341, the rinsing liquid area 342 and the eluent area 343 of the sample box 34 respectively, and proper lysate is added into the sample liquid, and the number of the sample boxes 34 is equal to the number of the magnetic rods;

the staff clamps the sample liquid region 341 of the sample box 34 inside the first placing plate 31, transversely clamps the eluent region 343 of the sample box 34 inside the second placing plate 32, then the heating film 35 starts to work, heats the sample box 34 inside the clamping groove, improves the mixing rate of the sample liquid and the pyrolysis liquid, and under the action of the heat insulation sleeve 36, the sample liquid region 341 is always kept at a sufficient temperature, namely, the heat inside the first placing plate 31 is ensured not to diffuse outwards, so that the heating effect of the sample liquid region 341 is better, the sample liquid can be quickly mixed with the pyrolysis liquid, the release rate of nucleic acid is improved, the rinsing liquid region 342 is positioned between the first placing plate 31 and the second placing plate 32 and is fully exposed to the outside, the heat insulation sleeve 36 does not insulate the region, the volatilization of the rinsing liquid and the waste of reagents caused by the too high heat at the region are avoided, and the directional heat insulation of the heat insulation sleeve 36 of the placing mechanism is realized;

then adding magnetic beads into the pyrolysis liquid, starting a third motor 422, rotating the output end of the third motor 422, starting the magnetic rod rack 45 to move downwards along a second sliding rail 44 under the transmission of a belt, enabling the magnetic rod to pass through a magnetic rod sleeve below the magnetic rod rack and extend to the liquid level of a sample box 34, starting to adsorb the magnetic beads by the magnetic rod positioned in a sample liquid area 341 at the moment, adsorbing nucleic acid, uniformly mixing the sample liquid with the pyrolysis liquid under the heat insulation function of a heat insulation sleeve 36, enabling the nucleic acid to be released more pure, ensuring the adsorption efficiency of the magnetic rod, reversely rotating the output end of a second motor 421, enabling the magnetic rod to move upwards and stay in the magnetic rod sleeve, rotating the output end of the second motor 421 at the moment, enabling the magnetic rod sleeve to move repeatedly upwards and downwards under the driving of the belt, vibrating, and driving the magnetic rod to vibrate, so that the magnetic beads adsorbed on the magnetic rod are collected into a block;

then the output end of the second motor 421 stops rotating, the output end of the first motor 411 rotates to drive the ball screw 43 to rotate, so that the movable bracket 42 moves rightwards, when the magnetic rod on the left side moves to the upper part of the rinsing liquid area 342, the output end of the first motor 411 stops rotating, and the output end of the third motor 422 rotates to enable the magnetic rod to move downwards to the inside of the rinsing liquid area 342, so that the magnetic beads contact the rinsing liquid for rinsing, and impurities are removed;

then the magnetic rod is reset again, along with the movement of the movable support 42 to the right above the eluent area 343, and the magnetic rod is eluted when falling and extending to the eluent again, so that the nucleic acid is separated from the magnetic beads and mixed into the eluent, meanwhile, the heat insulation sleeve 36 also covers the whole second placing plate 32, so that the inside of the second placing plate 32 also has enough heat, the heating effect of the eluent is enhanced, the elution efficiency of the magnetic rod is accelerated, further purified nucleic acid is obtained, the nucleic acid extraction process is completed, the heat insulation sleeve 36 insulates the second placing plate 32, the directional heat insulation function is reflected again, and the use mode is simple and convenient;

the embodiment specifically solves the problems that when the current nucleic acid extractor in the prior art is used, the sample seat only has the function of installing the sample box 34, has no heat insulation function, and the heat of the heating film 35 is easy to diffuse outwards through the sample box 34 and can not be concentrated in the sample box 34, so that the mixing rate of the lysate and the sample liquid is low, and the nucleic acid extraction efficiency is low; meanwhile, the rinse liquid is easy to volatilize when being heated greatly, so that the rinse liquid does not need to be heated and insulated, and the design of the sample seat capable of directionally insulating heat is a self-evident problem.

While certain exemplary embodiments of the present utility model have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the utility model. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the utility model, which is defined by the appended claims.

Claims (7)

1. A nucleic acid extractor with a heat insulation structure, characterized in that: the novel decorative plate comprises a shell (1), wherein the top of the bottom wall of the shell (1) is connected with a decorative plate (2), and two groups of placing mechanisms (3) which are distributed in parallel from left to right are arranged at the top of the decorative plate (2);

every group place mechanism (3) all include the first board (31) of placing that is connected with decorative board (2), first board (31) one side of placing is equipped with the second of being connected with decorative board (2) and places board (32), a plurality of slot (33) have all been seted up at first board (31) of placing and second board (32) top, slot (33) internally connected has sample box (34) that are used for storing sample liquid, rinsing liquid and eluent, decorative board (2) bottom is equipped with heating film (35) that are used for heating sample box (34), heating film (35) are connected with casing (1) diapire, first board (31) and second place board (32) outside and all are equipped with insulating jacket (36) that are connected with decorative board (2) for the heat that interval heating film (35) gives off realizes the lasting heat preservation of sample box (34), casing (1) rear side is equipped with extraction mechanism (4) that are used for extracting the inside nucleic acid of sample liquid.

2. The nucleic acid extractor having an insulating structure according to claim 1, wherein: the extraction mechanism (4) comprises two first sliding rails (41) which are distributed in parallel from front to back, the sliding rails are connected with the bottom wall of the shell, the top of each sliding rail is connected with a movable support (42), ball screws (43) are arranged at the bottoms of the movable supports (42) in a penetrating mode, second sliding rails (44) are connected to the movable front sides, magnetic rod frames (45) are connected to the tops of the second sliding rails (44), and magnetic rod sleeve frames (46) are connected to the bottoms of the second sliding rails (44).

3. The nucleic acid extractor having an insulating structure according to claim 1, wherein: the sample box (34) is formed by connecting a sample liquid area (341), a rinsing liquid area (342) and an eluent area (343) from left to right, the sample liquid area (341) is spliced at the top of the first placing plate (31) through a slot (33), and the eluent is spliced at the top of the second placing plate (32) through the slot (33).

4. The nucleic acid extractor having an insulating structure according to claim 1, wherein: the top slots (33) of each first placing plate (31) are longitudinally distributed from front to back, and the top slots (33) of the second placing plates (32) are correspondingly distributed with the tops of the first placing plates (31) one by one.

5. The nucleic acid extractor having an insulating structure according to claim 2, wherein: a first motor (411) is connected to one side of the bottom wall of the shell (1), and the output end of the first motor (411) is connected with a ball screw (43).

6. The nucleic acid extractor having an insulating structure according to claim 2, wherein: the bottom of the movable support (42) is connected with a second motor (421), the output end of the second motor (421) is in transmission connection with the magnetic rod sleeve frame (46) through a belt, the side wall of the top of the movable support (42) positioned on the second motor (421) is connected with a third motor (422), and the output end of the third motor (422) is in transmission connection with the magnetic rod frame (45) through a belt.

7. The nucleic acid extractor having an insulating structure according to claim 2, wherein: the bottom of the left side and the right side of the magnetic rod frame (45) is connected with a plurality of magnetic rods, the bottom of the left side and the right side of the magnetic rod sleeve frame (46) is connected with a plurality of magnetic rod sleeves, and the number of the magnetic rods is equal to that of the magnetic rod sleeves.

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