CN219409744U - Mixing heating device for nucleic acid extraction and nucleic acid extraction system - Google Patents

Abstract

The utility model discloses a mixing heating device for nucleic acid extraction and a nucleic acid extraction system, which realize the integrated arrangement of a sample mixing module and a sample heating module by arranging the sample mixing module and the sample heating module on the same bracket, not only have compact structures, but also have consumable materials arranged on a positioning frame of the sample mixing module, and can finish two processes of shaking and heating without transferring, thereby solving the problems that the prior art also needs to frequently transfer an extract between a mixing unit structure and a heating unit structure, further has low experimental efficiency and is easy to cause cross contamination. Nucleic acid extraction system has contained above-mentioned mixing heating device for the nucleic acid extraction, and the mixing heating device for the nucleic acid extraction's setting helps promoting the efficiency and the reliability of experimental data of whole nucleic acid extraction experiment, and the practicality is strong.

Description

Mixing heating device for nucleic acid extraction and nucleic acid extraction system

Technical Field

The utility model belongs to the technical field of biological research, relates to nucleic acid extraction equipment, and particularly relates to a mixing heating device for nucleic acid extraction and a nucleic acid extraction system.

Background

Nucleic acid is a basic unit representing genetic characteristics of a living body, and nucleic acid detection is advanced biological detection at a molecular level, and has the remarkable advantages of higher sensitivity, higher specificity, no window period and the like compared with the traditional morphological detection, cytological detection, immunological detection and the like.

With the continuous development of molecular diagnosis technology, a hospital department, a test center and the like have higher requirements on equipment automation. In the prior extraction experiments, most of nucleic acid is extracted by semi-automatic equipment, manual auxiliary operation is also needed, the extraction process is complex and tedious, and the whole experimental extraction process is completed for several hours. And, to the common extraction equipment in market, wherein mixing, heating are often independent unit structure, have taken up great space resource on the one hand, on the other hand with the extract frequently shift between mixing unit structure and heating unit structure, not only reduced experimental efficiency, easily take place cross contamination moreover to influence the reliability of experiment.

Disclosure of Invention

The utility model aims to provide a mixing heating device for nucleic acid extraction and a nucleic acid extraction system, which are used for solving the problems that the mixing unit structure and the heating unit structure of the existing extraction equipment are independently arranged, the occupied space is large, and the extract is required to be frequently transferred between the mixing unit structure and the heating unit structure, so that the experimental efficiency is low and cross contamination is easy to occur.

In order to achieve the above object, the present utility model provides the following solutions:

the utility model provides a mixing heating device for nucleic acid extraction, comprising:

a bracket;

the sample mixing module is arranged on the bracket and comprises a positioning frame and a mixing driving mechanism connected with the positioning frame, consumable materials capable of containing extracted samples are placed on the positioning frame, and the mixing driving mechanism is used for driving the positioning frame to move so as to shake and mix the extracted samples in the consumable materials;

and the sample heating module is arranged on the bracket and used for heating the extracted sample in the consumable.

Optionally, the consumptive material is the deep hole board, be provided with on the locating rack and supply the consumptive material passes the location slotted hole and can fix the mounting of deep hole board.

Optionally, the locating rack is located the support top, mixing actuating mechanism includes mixing driving motor, initiative polarization pole and driven polarization pole, the locating rack passes through one initiative polarization pole and at least one driven polarization pole with the top of support links to each other, wherein, initiative polarization pole and arbitrary one the top of driven polarization pole all pass through the bearing with the locating rack rotates to be connected, initiative polarization pole and arbitrary one driven polarization pole still pass through the bearing with the support rotates to be connected, mixing driving motor set up in on the support, and with the bottom of initiative polarization pole links to each other, mixing driving motor is used for the drive the locating rack is relative the support shakes.

Optionally, the fixing piece is a spring self-resetting buckle.

Optionally, the sample heating module is located the below of locating rack, the sample heating module include the heating block, set up in heating rod on the heating block and with the heating actuating mechanism that the heating block links to each other, the heating block is used for the heating draw the sample in the consumptive material, heating actuating mechanism is used for the drive the heating block is close to or keeps away from the bottom of consumptive material.

Optionally, an accommodating groove capable of being embedded in the bottom of the consumable is formed in the upper portion of the heating block; a plurality of heating rods are embedded in the lower part of the heating block; the heating driving mechanism is arranged below the heating block and is used for driving the heating block to lift relative to the locating frame, so that the containing groove is coated on the bottom of the consumable or away from the bottom of the consumable.

Optionally, the heating driving mechanism includes:

the lifting driving motor is arranged at the bottom of the bracket;

the bottom end of the screw rod is connected with the lifting driving motor;

the screw nut is in threaded connection with the screw, the top of the screw nut is connected with the heating block, and an avoidance hole for the screw to penetrate is formed in the bottom of the heating block;

the guide rod is parallel to the screw rod, the top end of the guide rod is connected with the heating block, and the bottom end of the guide rod is in sliding fit with the support.

Optionally, a heat insulation block is further arranged at the bottom of the heating block, and the top end of the guide rod is connected with the heat insulation block.

Optionally, the bracket comprises an upper support, a middle support and a lower support which are sequentially arranged at intervals from top to bottom, and any adjacent supports are connected through a support; the locating rack is arranged above the upper support, and the heating block is arranged between the upper support and the middle support.

Optionally, the heating rod comprises a heating rod body, a lifting driving motor and a mixing driving mechanism, and further comprises a control module which is electrically connected with the heating rod, the lifting driving motor and the mixing driving mechanism.

The utility model also provides a nucleic acid extraction system, which comprises a nucleic acid extraction device and the mixing and heating device for nucleic acid extraction.

Compared with the prior art, the utility model has the following technical effects:

according to the mixing and heating device for nucleic acid extraction, the sample mixing module and the sample heating module are arranged on the same support, so that the integrated arrangement of the sample mixing module and the sample heating module is realized, the structure is compact, consumable materials are arranged on the positioning frame of the sample mixing module, shaking and heating can be completed without transferring, and the problems that the experiment efficiency is low and cross contamination is easy to occur due to the fact that extracts are required to be frequently transferred between a mixing unit structure and a heating unit structure at present are solved.

In some technical schemes of the utility model, the support is arranged into a layered structure, so that layered arrangement of the sample mixing module and the sample heating module on the support is realized, the layout is reasonable, the structural compactness of the sample heating module and the sample mixing module after integration is further enhanced, and the space utilization rate is improved.

The nucleic acid extraction system provided by the utility model comprises the mixing heating device for nucleic acid extraction, and the mixing heating device for nucleic acid extraction is beneficial to improving the efficiency of the whole nucleic acid extraction experiment and the reliability of experimental data, and has strong practicability.

Drawings

In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram showing the overall structure of a mixing and heating device for nucleic acid extraction according to an embodiment of the present utility model;

FIG. 2 is a schematic diagram of a consumable part according to an embodiment of the present utility model;

fig. 3 is a schematic structural diagram of a positioning frame in a sample mixing module according to an embodiment of the present utility model;

fig. 4 is a schematic structural diagram of a sample mixing module according to an embodiment of the present utility model;

FIG. 5 is a schematic view showing a structure of a sample mixing module according to an embodiment of the present utility model, in which a housing of a rack is removed;

FIG. 6 is a schematic diagram of a sample heating module according to an embodiment of the present utility model;

FIG. 7 is a schematic view showing the structural arrangement of a middle-lower base of a bracket according to an embodiment of the present utility model;

fig. 8 is a schematic structural diagram of an active polarization rod according to an embodiment of the present utility model.

Wherein, the reference numerals are as follows:

100. a mixing and heating device for nucleic acid extraction;

1. a bracket; 11. an upper support; 12. a middle support; 13. a lower support; 14. an upper support column; 15. a lower support column; 16. a housing;

2. a sample mixing module; 21. a positioning frame; 22. positioning the slotted hole; 23. a fixing member; 24. uniformly mixing and driving a motor; 25. an active polarizing rod; 251. a first section bar; 252. a second section bar; 253. a lever connection; 26. a driven polarizing rod;

3. a sample heating module; 31. a heating block; 32. a heating rod; 33. a receiving groove; 34. a lifting driving motor; 35. a screw rod; 36. a lead screw nut; 37. a guide rod; 38. a heat insulating block; 39. a bearing seat;

4. a consumable;

5. a driving circuit board;

6. a heating rod connecting plate;

7. and heating the temperature control circuit board.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The utility model aims to provide a mixing heating device for nucleic acid extraction, which solves the problems that the mixing unit structure and the heating unit structure of the existing extraction equipment are independently arranged, the occupied space is large, and the extract is required to be frequently transferred between the mixing unit structure and the heating unit structure, so that the experimental efficiency is low and cross contamination is easy to occur.

Another object of the present utility model is to provide a nucleic acid isolation system having the above mixing heating device for nucleic acid isolation.

In order that the above-recited objects, features and advantages of the present utility model will become more readily apparent, a more particular description of the utility model will be rendered by reference to the appended drawings and appended detailed description.

Example 1

As shown in fig. 1, this embodiment provides a mixing heating device 100 for nucleic acid extraction, mainly including support 1, sample mixing module 2 and sample heating module 3, sample mixing module 2 sets up on support 1, sample mixing module 2 includes locating rack 21 and the mixing actuating mechanism that links to each other with locating rack 21, is used for placing on the locating rack 21 can hold the consumptive material 4 of extracting the sample, and mixing actuating mechanism is used for driving locating rack 21 and produces shake motion to make the extraction sample in the consumptive material 4 by shaking the mixing. The sample heating module 3 is arranged on the bracket 1 and is used for heating the extracted sample in the consumable 4. Through setting up sample mixing module 2 and sample heating module 3 on same support 1, realized the integration of sample mixing module 2 and sample heating module 3 and arranged, compact structure not only, consumable 4 sets up on sample mixing module 2's locating rack 21 moreover, need not to shift and can accomplish shaking even and heat twice process, has solved still need at present with the extract frequently shift between mixing unit structure and heating unit structure, and then causes experimental efficiency low, and easily take place cross contamination's problem.

In this embodiment, as shown in fig. 1 and 2, the consumable 4 is a deep hole plate, and the positioning frame 21 is provided with a positioning slot 22 through which the consumable 4 passes and a fixing piece 23 capable of fixing the deep hole plate. The deep pore plates have a plurality of structural forms, are classified according to the number of the pores, are classified into 48 pore plates, 96 pore plates, 384 pore plates and the like more commonly, are classified according to the hole types, are classified into round hole types and square hole types, are classified according to the shape of the hole bottom, and are mainly U-shaped (also called as round bottom) and V-shaped. The conventional deep-hole plate types can be applied to the technical scheme adaptively, and correspondingly, the arrangement form of the positioning slots 22 on the positioning frame 21 is also adjusted adaptively according to the different deep-hole plate types. Taking the consumable 4 as a 48-hole round-bottom deep hole plate as shown in fig. 2, the 48 round-bottom deep holes on the consumable 4 are distributed in 4 rows and 12 holes in each row, correspondingly, the positioning frame 21 is provided with 4 positioning slots 22 so as to respectively correspond to the 4 round-bottom deep holes on the 48-hole round-bottom deep hole plate, and the 4 deep holes of the 48-hole round-bottom deep hole plate respectively penetrate through the 4 positioning slots 22, so that the installation and the positioning of the consumable 4 on the positioning frame 21 are realized; the subsequent fixing piece 23 can be used for fixing the top plate edge of the 48-hole round bottom deep hole plate, so that the consumable 4 is fixed on the positioning frame 21. In order to achieve reliable fixation and facilitate disassembly and assembly of the consumable 4, the fixing piece 23 is preferably set as a spring self-resetting buckle in the embodiment; as shown in fig. 1 and 4, the spring self-resetting buckle comprises an L-shaped clamping piece, the corner position of the L-shaped clamping piece is used for clamping the consumable 4, the other end of the L-shaped clamping piece is hinged to the bottom edge of the positioning frame 21 through a rotating shaft, and a torsion spring is sleeved on the rotating shaft so as to keep the L-shaped clamping piece vertical relative to the positioning frame 21. When the consumable 4 needs to be installed, the consumable 4 is directly installed from top to bottom, when the consumable 4 touches the L-shaped clamping piece, the L-shaped clamping piece can be outwards Zhou Xietui, the consumable 4 is continuously installed downwards, when the consumable 4 reaches the corner position of the L-shaped clamping piece, the L-shaped clamping piece resets under the action of the torsion spring and is perpendicular to the positioning frame 21, and at the moment, the top plate of the consumable 4 is clamped into the corner position of the L-shaped clamping piece, so that the L-shaped clamping piece is fixed to the consumable 4. When the consumable 4 needs to be dismantled, the L-shaped clamping piece is outwards turned over along the rotating shaft of the L-shaped clamping piece, the clamping of the corner position of the L-shaped clamping piece to the consumable 4 is relieved, and then the consumable 4 is taken out. The spring self-resetting buckles are preferably uniformly arranged on the periphery of the positioning frame 21, for example, two pairs of springs are symmetrically arranged on two sides of the positioning frame 21, as shown in fig. 1.

In this embodiment, the positioning frame 21 is located above the support 1, the mixing driving mechanism includes a mixing driving motor 24, a driving polarizing rod 25 and a driven polarizing rod 26, the positioning frame 21 is connected with the top of the support 1 through a driving polarizing rod 25 and at least one driven polarizing rod 26, the tops of the driving polarizing rod 25 and any one driven polarizing rod 26 are rotationally connected with the positioning frame 21 through bearings, the driving polarizing rod 25 and any one driven polarizing rod 26 are rotationally connected with the support 1 through bearings, the mixing driving motor 24 is disposed on the support 1 and connected with the bottom end of the driving polarizing rod 25, and the mixing driving motor 24 is used for driving the driving polarizing rod 25 to rotate at a high speed. As shown in fig. 8, the active polarization rod 25 includes a first node rod 251 and a second node rod 252, the first node rod 251 and the second node rod 252 are axially connected by a rod connector 253, and the first node rod 251 and the second node rod 252 are arranged in parallel, but the axes of the first node rod 251 and the second node rod 252 are not coincident, i.e. the first node rod 251 and the second node rod 252 are eccentrically arranged, and the active polarization rod 25 is in a Z-shaped structure as a whole. The rod connector 253 may be a conventional connector structure such as a pipe hoop or a pipe joint. In the active polarization rod 25, the first rod 251 is shorter than the second rod 252, the first rod 251 is rotatably connected with the positioning frame 21 through a bearing, the second rod 252 is rotatably connected with the bracket 1 through a bearing, and the end part of the second rod 252 is connected with the mixing driving motor 24. The structure of each driven polarization rod 26 is a Z-shaped shaft like the driving polarization rod 25, namely, the shafts at the two ends of the driven polarization rods 26 are parallel and eccentrically arranged, one end shaft of each driven polarization rod 26 is rotationally connected with the bracket 1 through a bearing, and the other end shaft is rotationally connected with the positioning frame 21 through a bearing; the driven polarization rod 26 differs from the driving polarization rod 25 only in that the shaft section of the driven polarization rod 26 connected to the bracket 1 is shorter than the second joint rod 252. When the mixing driving motor 24 rotates at a high speed, the driving polarization rod 25 is driven to rotate integrally by taking the second section rod 252 as a center, and then the first section rod 251 drives the positioning frame 21 to rotate, and as the positioning frame 21 is further connected with the bracket 1 through the driven polarization rod 26, the rotation of the driving polarization rod 25 further drives each driven polarization rod 26 to rotate, and finally the positioning frame 21 moves eccentrically relative to the bracket 1 integrally under the action of the driving polarization rod 25 and each driven polarization rod 26, so that an extracted sample in the consumable 4 on the positioning frame 21 also moves eccentrically, and the extracted sample is driven by high-speed eccentric force to generate vortex phenomenon, so that the mixing effect is realized.

The positioning frame 21 is connected with the top of the bracket 1 through the driving polarizing rod 25 and at least one driven polarizing rod 26, the positioning frame 21 can be ensured to be stably supported above the bracket 1 by matching the driving polarizing rod 25 and the at least one driven polarizing rod 26, the positioning frame 21 is generally preferably of a rectangular plate structure, in order to further improve structural stability, the positioning frame 2 is preferably supported by adopting the driving polarizing rod 25 and the three driven polarizing rods 26, as shown in fig. 4, the driving polarizing rod 25 with longer length and the three driven polarizing rods 26 with shorter length are respectively installed at four corners of the positioning frame 21, and the vibration effect on the positioning frame 21 can be realized on the basis of stably supporting the positioning frame 21.

In this embodiment, the sample heating module 3 is located below the positioning frame 21, the sample heating module 3 includes a heating block 31, a heating rod 32 disposed on the heating block 31, and a heating driving mechanism connected to the heating block 31, where the heating block 31 is used to heat the extracted sample in the consumable 4, and the heating driving mechanism is used to drive the heating block 31 to approach or depart from the bottom of the consumable 4, and in general, when the consumable 4 needs to be heated, the heating driving mechanism drives the heating block 31 to approach the bottom of the consumable 4, and when the consumable 4 does not need to be heated, the heating driving mechanism drives the heating block 31 to depart from the bottom of the consumable 4, so as to avoid the heating block 31 from interfering with the consumable 4. The heating driving mechanism may be in various driving forms, and may move the heating block 31 horizontally or drive the heating block 31 up and down.

Further, the heating driving mechanism is preferably disposed below the heating block 31, and is mainly used for driving the heating block 31 to lift. As shown in fig. 6 and 7, in order to ensure that the sample in the consumable 4 is sufficiently heated, the upper portion of the heating block 31 is provided with accommodating grooves 33 capable of being embedded in the bottom of the consumable 4, where the number of the accommodating grooves 33 generally corresponds to the number of rows of deep holes in the consumable 4, for example, if the consumable 4 is the 48-hole round-bottom deep hole plate, the heating block 31 will be provided with 4 accommodating grooves 33 to accommodate the 4 rows of deep holes of the 48-hole round-bottom deep hole plate, i.e. each accommodating groove 33 corresponds to one row of deep holes. A plurality of heating rods 32 are embedded at the lower part of the heating block 31, and the bottom ends of all the heating rods 32 are fixed on the same heating rod connecting plate 6. The heating driving mechanism is disposed below the heating block 31 and is used for driving the heating block 31 to lift relative to the positioning frame 21, so that the accommodating groove 33 wraps the bottom of the consumable 4 or is far away from the bottom of the consumable 4. When the sample in the consumable 4 needs to be heated, the heating driving mechanism drives the heating block 31 to rise so that the accommodating groove 33 covers the bottom of the consumable 4, and the sample in each deep hole is heated; when the sample in the consumable 4 is not required to be heated, the heating driving mechanism drives the heating block 31 to descend, so that the whole consumable 4 is positioned on the upper portion of the heating block 31. As a lifting driving mechanism, the existing linear driving components such as a screw slider mechanism, an electric telescopic rod, a hydraulic cylinder or an air cylinder can be selected as the heating driving mechanism, and as a preferred scheme of the embodiment, the screw slider mechanism is selected as the heating driving mechanism, as shown in fig. 6 and 7, the heating driving mechanism comprises a lifting driving motor 34, a screw rod 35, a screw rod nut 36 and a guide rod 37, the lifting driving motor 34 is arranged at the bottom of the bracket 1, the bottom end of the screw rod 35 is connected with the lifting driving motor 34, the screw rod nut 36 is in threaded connection with the screw rod 35, the top of the screw rod nut 36 is connected with the bottom of the heating block 31, an avoidance hole for the screw rod 35 to penetrate is further formed in the bottom of the heating block 31, the diameter of the avoidance hole is larger than the diameter of the screw rod 35, so that the screw rod 35 is prevented from continuing to rotate after the screw rod 35 stretches into the avoidance hole, and the depth of the avoidance hole can be satisfied, so that the heating block 31 is separated from the bottom of the consumable 4. In addition, the avoidance holes are arranged so as to avoid the accommodating grooves 33 and the heating rods 32, and do not affect the normal heating function and deep hole accommodating function of the heating block 31. The guide rod 37 is arranged parallel to the screw rod 35, the top end of the guide rod 37 is connected with the bottom of the heating block 31, and the bottom end of the guide rod 37 is in sliding fit with the support 1. The lifting driving motor 34 drives the screw rod 35 to rotate forward or backward, so that the heating block 31 can be driven to slide up and down along the guide rod 37.

In this embodiment, the depth of the accommodating groove 33 on the heating block 31 is preferably based on a structure below the top plate of the consumable to ensure that the sample in the consumable is completely covered by the heating block 31 during heating. In the embodiment, the bottom of the heating block 31 is also provided with a heat insulation block 38, and the top end of the guide rod 37 is connected with the heat insulation block 38; the heat insulating block 38 is mainly used for blocking heat transfer from the heating block 31 to other components, and ensuring heating effect and heating efficiency of the sample.

In this embodiment, the bracket 1 is preferably a three-layer structure, as shown in fig. 5, which includes an upper support 11, a middle support 12 and a lower support 13 sequentially arranged at intervals from top to bottom, and any adjacent supports are connected through a support pillar, that is, the upper support 11 and the middle support 12 are connected through an upper support pillar 14, and the middle support 12 and the lower support 13 are connected through a lower support pillar 15. The upper support 11, the middle support 12 and the lower support 13 are all rectangular plate structures, and the upper support 11 and the middle support 12 are respectively supported by the support posts at four corners. The positioning frame 21 is arranged above the upper support 11, the heating block 31 is arranged between the upper support 11 and the middle support 12, in order to ensure that the heating block 31 can be in normal contact with the consumable 4, a hollow structure can be arranged at the center of the upper support 11 so as to expose the bottom of the consumable 4 placed on the heating block 31, and the positioning frame 21 is supported and connected with the edge of the upper support 11 through the driving polarization rod 25 and the driven polarization rod 26; correspondingly, the hollow structure is large enough, and the side wall of the hollow structure does not hinder the shaking of the consumable 4. Meanwhile, in order to improve the space utilization, the mixing driving motor 24 and the lifting driving motor 34 of the embodiment may be disposed on the middle support 12 or may be disposed on the lower support 13, and when the mixing driving motor 24 is disposed on the lower support 13, the active polarization rod 25 penetrates through the upper support 11 and the middle support 12 and then is connected with the output end of the mixing driving motor 24 through a coupling; similarly, the bottom end of the screw 35 penetrates through the middle support 12 and is connected with the output end of the lifting driving motor 34. The middle support 12 also plays a role of sliding fit with the guide rod 37, a bearing seat 39 is arranged on the middle support 12, and the guide rod 37 can be connected with the bearing seat 39 in a sliding way through a linear bearing; in order to improve the lifting stability of the heating block 31, two guide rods 37 are preferably disposed on two sides of the screw rod 35, and 4 guide rods 37 are provided, and each guide rod 37 has the same matching form with the middle support 12, which is not described herein. The heating block 31 is guided by the two guide rods 37 at the same time, so that the stability of the lifting process is improved. The support 1 of this embodiment sets up to foretell layered structure, has realized the layering arrangement of sample mixing module and sample heating module on support 1, and is rationally distributed not only, has further strengthened the compact structure after sample heating module and the integration of sample mixing module moreover, has promoted space utilization.

In this embodiment, three groups of sample heating modules may be arranged side by side on the stand 1, and the heating blocks 31 in the three groups of sample heating modules heat the consumable 4 together.

In practical operation, the support 1 may have various structural forms, the above-mentioned multi-layer structural arrangement form is only one expression form, and the specific structure of the support 1 may be adjusted and improved according to practical requirements. In addition, in order to ensure the whole device, particularly the dustproof effect of each motor in the device, a shell 16 can be arranged on the periphery of the frame body of the bracket 1, so that the device is attractive in appearance and dustproof, and multiple purposes are achieved.

In this embodiment, in order to further enhance the automation degree of the mixing heating device 100 for nucleic acid extraction, a control module may be configured, and the control module is electrically connected to the heating rod 32, the lifting driving motor 34 and the mixing driving motor 24. For example, a driving circuit board 5 and a heating temperature control circuit board 7 are arranged in the control module, and the driving circuit board 5 can control the start and stop, the rotating speed and the steering of the lifting driving motor 34 and the mixing driving motor 24; the heating temperature control circuit board 7 can cooperate with a temperature sensor and other detection modules to control the heating temperature of each heating rod 32 so as to ensure that the sample in the consumable 4 is heated within a reasonable temperature range and avoid the condition of excessive heating failure or insufficient heating of the sample. The heating rods 32 are of a conventional heating structure, such as resistance wire heating rods, the heating rods 32 are preferably uniformly distributed in an array in the heating block 31, the heating rods 32 on the same heating block 31 are welded on the same heating rod connecting plate 6, and the heating rod connecting plate 6 is preferably fixed at the bottom of the heating block 31.

The method of using the mixing and heating device 100 for nucleic acid isolation according to this embodiment will be specifically described below.

The consumable 4 containing the nucleic acid extraction sample is mounted on the positioning frame 21, and then the consumable 4 is fixed by the fixing member 23. Then, the heating block 31 can be driven to rise by the lifting driving motor 34, so that the containing groove 33 on the heating block 31 covers the bottom of the deep hole of the consumable 4, and then the heating rod 32 is started to heat the sample in the consumable 4. After the heating is completed, the heating block 31 is driven to descend by the lifting driving motor 34, so that the heating block 31 is evacuated from the bottom of the consumable 4. Then the mixing driving motor 24 can be started to shake the positioning frame 21 so as to uniformly mix the samples in the consumable 4 under the shaking action. In actual operation, the samples may be uniformly mixed first, and then heated by the heating block 31, and the specific operation procedure may be adjusted according to actual requirements.

Therefore, it can be seen that the mixing heating device 100 for nucleic acid extraction provided by the technical scheme is a mixing heating integrated device, which is characterized in that the sample mixing module and the sample heating module are arranged on the same support, so that the integrated arrangement of the sample mixing module and the sample heating module is realized, the structure is compact, consumables are arranged on a positioning frame of the sample mixing module, shaking and heating two procedures can be completed without transferring, and the problems that extracts are required to be frequently transferred between a mixing unit structure and a heating unit structure at present, experimental efficiency is low and cross contamination is easy to occur are solved. The support is arranged to be of a layered structure, layered arrangement of the sample mixing module and the sample heating module on the support is achieved, the layout is reasonable, the structural compactness of the sample heating module and the sample mixing module after integration is further improved, and the space utilization rate is improved.

According to the mixing heating device 100 for nucleic acid extraction, provided by the technical scheme, through configuration of the corresponding control module, full-automatic operation of sample heating and mixing can be realized, the operation process is simple and convenient, the efficiency is high, the problem that the experimental efficiency is low because the existing extraction equipment is semi-automatic operation and manual operation assistance is needed is avoided.

Example two

The embodiment provides a nucleic acid extraction system, which comprises a nucleic acid extraction device, a mixing and heating device 100 for nucleic acid extraction as disclosed in the first embodiment, and a consumable 4. The mixing and heating device 100 for nucleic acid extraction is only one ring in the whole molecular diagnosis nucleic acid extraction experiment, and is mainly used for mixing and heating extracted nucleic acid, so as to realize sample mixing and heating integration.

It should be noted that it will be apparent to those skilled in the art that the present utility model is not limited to the details of the above-described exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

The principles and embodiments of the present utility model have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present utility model; also, it is within the scope of the present utility model to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the utility model.

Claims (10)

1. A mixing and heating device for nucleic acid extraction, comprising:

a bracket;

the sample mixing module is arranged on the bracket and comprises a positioning frame and a mixing driving mechanism connected with the positioning frame, consumable materials capable of containing extracted samples are placed on the positioning frame, and the mixing driving mechanism is used for driving the positioning frame to move so as to shake and mix the extracted samples in the consumable materials;

and the sample heating module is arranged on the bracket and used for heating the extracted sample in the consumable.

2. The mixing and heating device for nucleic acid extraction according to claim 1, wherein the consumable is a deep hole plate, and the positioning frame is provided with a positioning slot hole through which the consumable passes and a fixing piece capable of fixing the deep hole plate.

3. The mixing and heating device for nucleic acid extraction according to claim 2, wherein the positioning frame is located above the support, the mixing driving mechanism comprises a mixing driving motor, a driving polarizing rod and a driven polarizing rod, the positioning frame is connected with the top of the support through the driving polarizing rod and at least one driven polarizing rod, the tops of the driving polarizing rod and any driven polarizing rod are rotatably connected with the positioning frame through bearings, the driving polarizing rod and any driven polarizing rod are rotatably connected with the support through bearings, the mixing driving motor is arranged on the support and is connected with the bottom end of the driving polarizing rod, and the mixing driving motor is used for driving the positioning frame to shake relative to the support.

4. The mixing and heating device for nucleic acid extraction as defined in claim 2, wherein the fixing member is a spring self-resetting buckle.

5. The mixing and heating device for nucleic acid extraction according to any one of claims 2 to 4, wherein the sample heating module is located below the positioning frame, and comprises a heating block, a heating rod arranged on the heating block, and a heating driving mechanism connected with the heating block, wherein the heating block is used for heating an extracted sample in the consumable, and the heating driving mechanism is used for driving the heating block to be close to or far away from the bottom of the consumable.

6. The mixing and heating device for nucleic acid extraction as defined in claim 5, wherein a receiving groove capable of being inserted into the bottom of the consumable is provided at the upper part of the heating block; a plurality of heating rods are embedded in the lower part of the heating block; the heating driving mechanism is arranged below the heating block and is used for driving the heating block to lift relative to the locating frame, so that the containing groove is coated on the bottom of the consumable or away from the bottom of the consumable.

7. The mixing and heating device for nucleic acid isolation according to claim 6, wherein the heating and driving mechanism comprises:

the lifting driving motor is arranged at the bottom of the bracket;

the bottom end of the screw rod is connected with the lifting driving motor;

the screw nut is in threaded connection with the screw, the top of the screw nut is connected with the heating block, and an avoidance hole for the screw to penetrate is formed in the bottom of the heating block;

the guide rod is parallel to the screw rod, the top end of the guide rod is connected with the heating block, and the bottom end of the guide rod is in sliding fit with the support.

8. The mixing and heating device for nucleic acid extraction according to claim 7, wherein a heat insulating block is further provided at the bottom of the heating block, and the tip of the guide rod is connected to the heat insulating block.

9. The mixing and heating device for nucleic acid extraction according to claim 5, wherein the support comprises an upper support, a middle support and a lower support which are sequentially arranged at intervals from top to bottom, and any adjacent supports are connected through a support column; the locating rack is arranged above the upper support, and the heating block is arranged between the upper support and the middle support.

10. A nucleic acid extraction system comprising a nucleic acid extraction device and a mixing and heating device for nucleic acid extraction according to any one of claims 1 to 9.