CN216039618U

CN216039618U - Nucleic acid extraction instrument

Info

Publication number: CN216039618U
Application number: CN202122113429.XU
Authority: CN
Inventors: 汪昆; 郭敬舜; 林小靖; 曹健荣
Original assignee: Denogen Beijing Bio Sci&tech Co ltd
Current assignee: Denogen Beijing Bio Sci&tech Co ltd
Priority date: 2021-09-02
Filing date: 2021-09-02
Publication date: 2022-03-15
Anticipated expiration: 2031-09-02

Abstract

The utility model discloses a nucleic acid extraction instrument, which comprises a driving assembly and a reagent bin module, wherein the driving assembly comprises a magnetic rod assembly and a magnetic sleeve assembly, the magnetic sleeve assembly comprises a magnetic rod sleeve, a magnetic sleeve driving structure is connected onto the magnetic rod sleeve, the magnetic sleeve driving structure is directly connected with a second driving assembly, and the second driving assembly directly drives the magnetic sleeve driving structure to move; the bar magnet subassembly includes the bar magnet, be connected with bar magnet bearing structure on the bar magnet, the extrusion is provided with the actuating arm on the bar magnet bearing structure, the below of actuating arm is provided with bar magnet drive structure, bar magnet drive structure and first drive assembly lug connection, the surface of bar magnet drive structure is connected for the contact with the surface of actuating arm, first drive assembly drive bar magnet drive structure rebound, the indirect actuating arm that supports drives the bar magnet bearing structure and shifts up, bar magnet bearing structure relies on bar magnet subassembly self gravity downstream. The nucleic acid extractor has the advantages of safe and reliable driving effect, small size and high heating efficiency, and is not easy to damage.

Description

Nucleic acid extraction instrument

Technical Field

The utility model relates to the field of genetic engineering instruments and equipment, in particular to a nucleic acid extractor.

Background

The nucleic acid extractor is an instrument for automatically finishing the extraction work of sample nucleic acid by using a matched nucleic acid extraction reagent, and is widely applied to various fields of disease control centers, clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, animal husbandry, molecular biology research and the like. Nucleic acid extraction comprises the following basic steps: 1. cracking: adding lysis solution into the sample, and mechanically moving and heating to uniformly mix and fully react the reaction solution, so that cells are lysed, and nucleic acid is released; 2. adsorption: adding magnetic beads into a sample lysis solution, fully and uniformly mixing, adsorbing nucleic acid by utilizing the characteristic that the magnetic beads have strong affinity to the nucleic acid under high salt and low pH values, and separating the magnetic beads from the lysis solution under the action of an external magnetic field; 3. washing: transferring the magnetic beads adsorbed with the nucleic acid into a new washing buffer solution, fully and uniformly mixing, washing away impurities, and separating the magnetic beads from the washing buffer solution under the action of an external magnetic field; 4. and (3) elution: and transferring the magnetic beads into an elution buffer, removing the external magnetic field to fully and uniformly mix the magnetic beads and the eluent, separating the combined nucleic acid from the magnetic beads, and mixing the nucleic acid into the elution buffer to obtain the purified nucleic acid.

The existing nucleic acid extraction instrument generally adopts a magnetic bead separation technology of a magnetic rod method, realizes the adsorption and transfer of magnetic beads by utilizing the mutual matching motion of a magnetic rod and a magnetic rod sleeve, simultaneously utilizes the magnetic rod sleeve to repeatedly stir liquid, fully mixes a reaction liquid system, and finally obtains purified nucleic acid through steps of sample cracking, nucleic acid adsorption to the magnetic beads, magnetic bead washing, nucleic acid elution and the like. In schizolysis and elution step, need to heat schizolysis reagent and elution reagent, can set up heating device among the current nucleic acid extraction appearance usually, when carrying out nucleic acid extraction, place the kit in the reagent storehouse of nucleic acid extraction appearance, make heating device correspond on the position of the lysate and the eluant that the kit splendid attire has for heat.

In the existing nucleic acid extractor, the magnetic rod and the magnetic rod sleeve are respectively directly connected with two power elements, and the magnetic rod sleeve synchronously move, synchronously vibrate, independently move or vibrate through respective direct drive of the two power elements. However, this driving method has some problems, and since the magnetic rod and the magnetic rod sleeve need to have precise motion matching in each step, once the control system or the detection system of the instrument fails or the motion precision of the motion mechanism of the instrument is deteriorated after long-time operation, the magnetic rod may excessively move towards the magnetic rod sleeve, and collide with each other, thereby damaging the instrument and even causing accidents. Particularly, when the magnetic rod and the magnetic rod sleeve need to move together or vibrate together, the drives of the two groups of power elements are required to keep absolute synchronization, so that the design difficulty of hardware and software of the instrument is increased, the instrument is inconvenient to implement, and the problem of excessive movement of the magnetic rod relative to the magnetic rod sleeve is more likely to occur, so that danger is generated.

In addition, because the amount of reagents used in the two steps of lysis and elution are different, the heating requirements of the two steps are different, and the heating structure and the heating mode of the existing heating device used in the steps of lysis and elution are the same, the heating effect of the heating device is obviously insufficient for the lysis step, and the heating effect of the heating device is obviously excessive for the elution step, so that the problems of low heating efficiency and low nucleic acid extraction speed are caused. Because the heating time required by the cracking step is longer, the heat of the cracking area can be more radiated to the nearby experimental areas such as washing, elution and the like, and the experimental effects of other steps are influenced; and in the elution step, the liquid volume of elution reagent is less, and the heating device heat capacity is too big can cause the excessive volatilization of eluant, and extravagant reagent influences reagent concentration, even in the oscillation process, leads to the magnetic bead to be heated the evaporation and dry knot on the inner wall of test tube, makes the unable normal solution of nucleic acid on the magnetic bead in the eluant, influences sample quality.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a nucleic acid extraction instrument, which is characterized in that a special driving structure and a special heating assembly are arranged, so that the movement of a magnetic rod relative to a magnetic rod sleeve is safer and more controllable, accidents are avoided, meanwhile, different heating structures are arranged according to different heating requirements of cracking and elution steps, the heating efficiency is improved, and adverse effects caused by heat radiation are reduced. The specific technical scheme is as follows:

a nucleic acid extraction instrument comprises a driving assembly and a reagent bin module, wherein the driving assembly comprises a magnetic rod assembly and a magnetic sleeve assembly, the magnetic rod assembly and the magnetic sleeve assembly are arranged above the reagent bin module, the magnetic sleeve assembly comprises a magnetic rod sleeve, a magnetic sleeve driving structure is connected onto the magnetic rod sleeve, the magnetic sleeve driving structure is directly connected with a second driving assembly, and the second driving assembly directly drives the magnetic sleeve driving structure to move; the bar magnet subassembly includes the bar magnet, be connected with bar magnet bearing structure on the bar magnet, the extrusion is provided with the actuating arm on the bar magnet bearing structure, the below of actuating arm is provided with bar magnet drive structure, bar magnet drive structure and first drive assembly lug connection, the surface of bar magnet drive structure is connected for the contact with the surface of actuating arm, first drive assembly drive bar magnet drive structure rebound, the indirect actuating arm that supports drives the bar magnet bearing structure and shifts up, bar magnet bearing structure relies on bar magnet subassembly self gravity downstream.

Furthermore, the magnetic bar is fixedly connected to the magnetic bar frame, the magnetic bar sleeve is detachably arranged on the magnetic bar sleeve frame, the magnetic bar bearing structure comprises a magnetic bar arm and a magnetic bar bearing block, the magnetic bar arm comprises a first connecting arm arranged along the transverse direction and a second connecting arm arranged along the vertical direction, the first connecting arm is fixedly connected with the magnetic bar frame, and the second connecting arm is fixedly connected with the magnetic bar bearing block; the magnetic sleeve driving structure comprises a magnetic sleeve arm and a magnetic sleeve driving block, the magnetic sleeve arm comprises a third connecting arm arranged along the transverse direction and a fourth connecting arm arranged along the vertical direction, the third connecting arm is fixedly connected with the magnetic sleeve frame, and the fourth connecting arm is fixedly connected with the magnetic sleeve driving block; the first connecting arm and the third connecting arm are oppositely arranged.

Furthermore, the first driving assembly comprises a first motor and a first lead screw, the second driving assembly comprises a second motor and a second lead screw, the first motor and the second motor are fixedly arranged on the base assembly, one end of the first lead screw is connected with the first motor, the other end of the first lead screw extends in the direction far away from the base assembly along the vertical direction, one end of the second lead screw is connected with the second motor, and the other end of the second lead screw extends in the direction far away from the base assembly along the vertical direction; the base assembly is movably connected with the instrument bottom plate, a third lead screw is connected onto the base assembly, the third lead screw extends along the transverse direction, a third motor is connected onto the third lead screw, and the third motor can drive the third lead screw to drive the base assembly to move on the instrument bottom plate.

Further, the base assembly comprises an upper base plate and a lower base plate, the upper base plate is located above the lower base plate, a side support plate and two side supports are arranged between the upper base plate and the lower base plate, the side support plate is connected with a first end portion of the upper base plate and a first end portion of the lower base plate, the two side supports are connected with a second end portion of the upper base plate and a second end portion of the lower base plate, an accommodating space is formed between the upper base plate and the lower base plate, the first motor, the second motor and the third motor are arranged in the accommodating space, the first lead screw and the second lead screw penetrate through the upper base plate to extend upwards, the magnetic rod driving structure is arranged on the first lead screw, the magnetic sleeve driving structure is arranged on the second lead screw, a gap is formed between the two side supports, a support is arranged in the gap, the support is fixedly arranged on the instrument base plate, one end of the third lead screw is connected with the support, and the other end of the support is connected with the support.

Further, a first guide shaft and a second guide shaft are arranged on the base assembly, the first guide shaft and the first lead screw are parallel and arranged side by side, one end of the first guide shaft is fixedly connected with the upper bottom plate, the other end of the first guide shaft extends towards the upper part of the base assembly along the vertical direction, a second guide hole is formed in the magnetic bar bearing structure, the second guide hole is sleeved on the first guide shaft, and the magnetic bar bearing structure can drive the magnetic bar to slide up and down along the first guide shaft; the magnetic sleeve driving structure is provided with a first guide hole, the first guide hole is sleeved on a first guide shaft, so that a guiding effect is achieved on the magnetic sleeve driving structure, one end of a second guide shaft is fixedly connected with an upper base plate, the other end of the second guide shaft extends towards the upper portion of a base assembly along the vertical direction, the second guide shaft is parallel to a second lead screw and is arranged side by side, a third guide hole is formed in the magnetic rod driving structure, and the third guide hole is sleeved on the second guide shaft and achieves a guiding effect on the magnetic rod driving structure.

Further, including infrared detection subassembly, infrared detection subassembly includes first photoelectric separation blade, second photoelectric separation blade and third photoelectric separation blade, and with first photoelectric separation blade, the sensor that second photoelectric separation blade and third photoelectric separation blade cooperation set up, first photoelectric separation blade sets up on base subassembly, can remove along the horizontal direction along with base subassembly, second photoelectric separation blade sets up on magnetic sleeve drive structure, can reciprocate along with magnetic sleeve drive structure, third photoelectric separation blade sets up structurally at the magnetic rod drive, can reciprocate along with magnetic rod drive structure.

Further, reagent storehouse module includes heating element, heating element is including the base that is used for bearing nucleic acid to draw the kit, be provided with first heating member and second heating member on the base, the first heating member corresponds the schizolysis station test tube setting of kit, the second heating member corresponds the elution station test tube setting of kit, be formed with first heating tank on the first heating member, be formed with the second heating tank on the second heating member, the degree of depth in first heating tank is greater than the degree of depth in second heating tank, the degree of depth in first heating tank and the liquid level phase-match in the schizolysis station test tube, the degree of depth in second heating tank and the liquid level phase-match in the elution station test tube.

Further, first heating member is including the first heat supply portion and the first portion of generating heat that are connected, the second heating member is including the second heat supply portion and the second portion of generating heat that are connected, a plurality of first heating tank formation are in first heat supply portion, a plurality of second heating tank formation are in second heat supply portion, the length of first heat supply portion is greater than the length of second heat supply portion, be formed with first logical groove in the first portion of generating heat, be formed with the second in the second portion of generating heat and lead to the groove, be provided with heating element in first logical groove and the second logical groove.

Further, a plurality of first hollow structures and a plurality of second hollow structures are formed on the first heat supply part, the first hollow structures are arranged between two adjacent first heating grooves, and the second hollow structures are located on the side walls of the two sides of the first heat supply part and symmetrically arranged on the two sides of each first heating groove; be formed with a plurality of heating posts in the first heat supply portion, the heating post is located between two adjacent schizolysis station test tubes, the shape phase-match in the gap that forms between the shape of heating post and two adjacent schizolysis test tubes.

Further, the side walls of the first heating body and the second heating body are provided with temperature measuring holes and temperature measuring channels, temperature measuring probes are arranged in the temperature measuring holes, the temperature measuring channels are communicated with the temperature measuring holes, and the wiring of the temperature measuring probes is distributed along the temperature measuring channels; the base is provided with at least two mounting grooves, the first heating body and the second heating body are arranged in different mounting grooves, a fixing clamp is arranged below the base, a clamping groove is formed in the fixing clamp, and the clamping groove is clamped below the first heating part and the second heating part and is fixedly connected with the lower surface of the base; a lower concave part is arranged in the clamping groove, a heat preservation device is arranged in the lower concave part, and the heat preservation device is electrically connected with the heating element in the first heating body and/or the second heating body so as to control the heating element to be powered on or powered off.

The nucleic acid extraction instrument of the utility model has the following advantages:

1. the magnetic rod assembly and the magnetic sleeve assembly can be prevented from being in hard contact, and damage or accidents caused by collision of the magnetic rod assembly and the magnetic sleeve assembly are avoided;

2. the complexity of hardware and software of the nucleic acid extractor is simplified;

3. the driving assembly has a compact structure, the volume and the occupied space of the instrument are greatly reduced, and the driving effect of the driving assembly is improved, so that the movement process is safer and more reliable, and the damage or the accident is not easy to occur;

4. the heating efficiency of the heating assembly is improved, the heat radiation which is not beneficial to the nucleic acid extraction effect is reduced, the excessive volatilization of the reagent is reduced, the stability of the concentration of the reagent is ensured, and the efficiency and the extraction quality of the nucleic acid extraction are improved;

5. the assembly precision of the heating component is high, the position deviation or the vertical dislocation is not easy to occur, the matching precision of the kit and the heating device is high, and the quality and the efficiency of nucleic acid extraction are favorably improved.

Drawings

FIG. 1 is a perspective view of the nucleic acid isolation apparatus of the present invention.

FIG. 2 is a schematic view of the internal structure of the nucleic acid isolation apparatus of the present invention.

FIG. 3 is a first perspective view of a driving unit of the nucleic acid isolation apparatus according to the present invention.

FIG. 4 is a second perspective view of the driving unit of the nucleic acid isolation apparatus of the present invention.

FIG. 5 is a third perspective view of the driving unit of the nucleic acid isolation apparatus of the present invention.

Fig. 6 is a first schematic view of the assembled state of the magnetic rod assembly and the magnetic sleeve assembly in the utility model.

FIG. 7 is a second schematic view of the assembled magnetic rod assembly and magnetic sleeve assembly of the present invention.

FIG. 8 is a first perspective view of a magnetic rod assembly according to the present invention.

FIG. 9 is a second perspective view of the magnetic rod assembly of the present invention.

Fig. 10 is a first perspective view of a magnetic sleeve assembly of the present invention.

Fig. 11 is a second perspective view of the magnetic sleeve assembly of the present invention.

FIG. 12 is a first perspective view of a heating unit of the nucleic acid isolation apparatus according to the present invention.

FIG. 13 is a front view of a heating unit of the nucleic acid isolation apparatus according to the present invention.

FIG. 14 is an exploded view of a heating unit of the nucleic acid isolation instrument according to the present invention.

FIG. 15 is a second perspective view of the heating unit of the nucleic acid isolation apparatus of the present invention.

FIG. 16 is a third perspective view of the heating unit of the nucleic acid isolation apparatus of the present invention.

Fig. 17 is a first perspective view of the first and second heating bodies in the present invention.

Fig. 18 is a second perspective view of the first and second heating bodies in the present invention.

Fig. 19 is a perspective view of a first retainer card and a second retainer card of the present invention.

FIG. 20 is a schematic bottom view of a heating unit of the nucleic acid isolation apparatus according to the present invention.

FIG. 21 is a front sectional view of a heating unit of the nucleic acid isolation instrument according to the present invention.

Detailed Description

In order to better understand the purpose, structure and function of the present invention, the nucleic acid extractor of the present invention will be described in further detail with reference to the accompanying drawings.

The nucleic acid extractor adopts a magnetic bead separation technology of a magnetic rod method, magnetic beads are adsorbed and transferred by using a magnetic rod, liquid is repeatedly stirred by using a magnetic rod sleeve, a reaction liquid system is fully and uniformly mixed, and purified nucleic acid is finally obtained through steps of sample cracking, nucleic acid adsorption to the magnetic beads, magnetic bead washing, nucleic acid elution and the like.

Specifically, as shown in fig. 1, the nucleic acid extraction instrument of the present invention includes a housing 1, wherein an accommodating chamber is formed in the housing 1, and each functional module of the nucleic acid extraction instrument is disposed in the housing 1; the casing 1 is provided with a door plate 2, and the door plate 2 can rotate relative to the casing 1 to open or close the accommodating chamber so as to put in or take out the kit for extracting nucleic acid; the housing 1 is also provided with a display screen 3 for operating and displaying data and the working state of the instrument, so that a user can conveniently control and observe the instrument.

Further, as shown in fig. 2, a power module (not shown in the figure), a driving assembly 4 and a reagent bin module 5 are arranged in the accommodating chamber of the nucleic acid extractor, the reagent bin module 5 is arranged close to the driving assembly 4, the power module is arranged close to the driving assembly 4, wherein a magnetic rod assembly 6 and a magnetic sleeve assembly 7 are arranged on the driving assembly 4, and the power module can provide power for the driving assembly 4 so that the driving assembly 4 drives the magnetic rod assembly 6 and the magnetic sleeve assembly 7 to move or vibrate. When nucleic acid extraction is carried out, a kit (not shown in the figure) is placed on the reagent bin module 5, a plurality of stations are arranged in the kit and respectively contain reagents for nucleic acid extraction, such as lysis solution, washing buffer solution, eluent and the like, the driving assembly 4 drives the magnetic rod assembly 6 and the magnetic sleeve assembly 7 to move or vibrate at the stations containing different reagents so as to adsorb and transfer magnetic beads, stir and mix liquid uniformly, and finally, purified nucleic acid is extracted from a sample.

Further, as shown in FIGS. 3 to 5, the driving unit 4 of the nucleic acid isolation apparatus of the present invention includes a base unit movably disposed on the apparatus base of the nucleic acid isolation apparatus and horizontally slidable in a linear direction on the apparatus base. The base component is provided with a magnetic rod component 6 and a magnetic sleeve component 7, and the magnetic sleeve component 7 is positioned below the magnetic rod component 6; the magnetic bar component 6 is movably connected with the base component through a first driving component, and the first driving component can drive the magnetic bar component 6 to move up and down in the vertical direction relative to the base component; the magnetic sleeve assembly 7 is movably connected with the base assembly through a second driving assembly, and the second driving assembly can drive the magnetic sleeve assembly 7 to move up and down in the vertical direction relative to the base assembly. The base component is also provided with a third driving component, and the base component can be driven by the third driving component to linearly slide along the guide rail on the instrument bottom plate.

Specifically, as shown in fig. 4 and 5, the base assembly includes an upper base plate 11, a lower base plate 12, side supporting plates 13 and side supporting columns 14, the upper base plate 11 is located above the lower base plate 12, the side supporting plates 13 are located between the upper base plate 11 and the lower base plate 12, and a first end of the upper base plate 11 is fixedly connected with a first end of the lower base plate 12; two side pillars 14 are provided between the second end of the upper plate 11 and the second end of the lower plate 12 to fixedly connect the second ends of the upper and lower plates 12. The upper bottom plate 11, the lower bottom plate 12, the side supporting plates 13 and the side supporting columns 14 jointly enclose a frame structure, and an accommodating space is formed inside the frame structure; the lower base plate 12 is movably arranged on a guide rail of the instrument base plate, and the lower base plate 12 slides along the guide rail, so that the whole base assembly can slide on the instrument base plate.

Specifically, as shown in fig. 3, 6 and 7, the magnetic sleeve assembly 7 includes a magnetic sleeve frame 71, a sliding slot 72 is disposed on the magnetic sleeve frame 71, a magnetic rod sleeve (not shown in the figure) is detachably disposed in the sliding slot 72, and the magnetic rod sleeve extends in the vertical direction and is suspended above the reagent cartridge module 5; one end of the magnetic sleeve frame 71 is connected with a magnetic sleeve arm 73, the magnetic sleeve arm 73 is connected with a magnetic sleeve driving block 74, and the magnetic sleeve driving block 74 is connected with a second driving assembly; the second driving assembly comprises a second motor and a second screw rod, the second motor is fixedly arranged on the lower bottom plate 12 of the base assembly and is positioned in the accommodating space of the base assembly, one end of the second screw rod is connected with the second motor, and the other end of the second screw rod penetrates out of a second through hole in the upper bottom plate 11 of the base assembly and extends in the direction far away from the base assembly along the vertical direction; the magnetic sleeve driving block 74 is connected with the second lead screw through a thread structure, and the magnetic sleeve driving block 74 can drive the magnetic sleeve arm 73, the magnetic sleeve frame 71 and the magnetic bar sleeve to move up and down along the second lead screw under the driving of the second motor.

Preferably, a first guide shaft 81 is arranged on the base assembly, one end of the first guide shaft 81 is fixedly connected with the upper bottom plate 11, and the other end of the first guide shaft 81 extends towards the upper part of the base assembly along the vertical direction; correspondingly, a first guide hole is formed in the magnetic sleeve driving block 74, and the first guide hole is sleeved on the first guide shaft 81, so that the magnetic sleeve driving block 74 can slide up and down along the first guide shaft 81, and further, the movement of the magnetic sleeve driving block 74 is guided.

Preferably, the base assembly is provided with two first guide shafts 81, the two first guide shafts 81 are symmetrically arranged on two sides of the second lead screw, and correspondingly, the magnetic sleeve driving block 74 is provided with two first guide holes which are respectively sleeved on the two first guide shafts 81. This arrangement further enhances the guiding effect and makes the movement of the magnetic sleeve driving block 74 more stable and reliable.

Specifically, as shown in fig. 3, 6 and 7, the magnetic rod assembly 6 includes a magnetic rod rack 61, a plurality of magnetic rods 62 for generating magnetic force and adsorbing magnetic beads are fixedly disposed on the magnetic rod rack 61, the magnetic rod rack 61 and the magnetic rods 62 are located above a magnetic sleeve rack 71 and a magnetic rod sleeve, and the magnetic rod sleeve can be sleeved outside the magnetic rods 62; one end of the magnetic bar frame 61 is connected with a magnetic bar arm 63, the magnetic bar arm 63 is connected with a magnetic bar bearing block 64, the magnetic bar arm 63 is positioned above the magnetic sleeve arm 73, and the magnetic bar bearing block 64 is positioned above the magnetic sleeve driving block 74; the magnetic bar bearing block 64 is provided with two second guide holes, and the two second guide holes are respectively sleeved on the two first guide shafts 81, so that the magnetic bar bearing block 64 can drive the magnetic bar arm 63, the magnetic bar frame 61 and the magnetic bar 62 to slide up and down along the first guide shafts 81 together.

The first driving assembly comprises a first motor and a first lead screw, the first motor is fixedly arranged on the lower bottom plate 12 of the base assembly, is positioned in the accommodating space of the base assembly and is close to the second motor; one end of the first lead screw is connected with the first motor, the other end of the first lead screw penetrates out of the first through hole in the upper base plate 11 of the base assembly, and the first lead screw extends along the vertical direction towards the direction far away from the base assembly. A magnetic bar driving block 65 is arranged on the first lead screw, and the magnetic bar driving block 65 is connected with the first lead screw through a threaded structure; the magnetic bar driving block 65 is arranged close to the magnetic bar bearing block 64 and located below the magnetic bar bearing block 64, when the magnetic bar driving block 65 moves upwards along the first lead screw under the driving of the first motor, the upper surface of the magnetic bar driving block 65 is abutted against the magnetic bar bearing block 64 and supports the magnetic bar bearing block 64 to slide upwards along the first guide shaft 81, and then the magnetic bar arm 63, the magnetic bar frame 61 and the magnetic bar 62 are driven to move upwards along the first guide shaft 81; when the magnetic bar driving block 65 moves downwards along the first lead screw under the driving of the first motor, the magnetic bar bearing block 64 slides downwards along the first guide shaft 81 by means of the self-gravity, so that the magnetic bar arm 63, the magnetic bar frame 61 and the magnetic bar 62 move downwards together.

Preferably, the magnetic rod bearing block 64 is provided with a driving arm 66, the driving arm 66 is arranged to protrude from the surface of the magnetic rod bearing block 64, the magnetic rod driving block 65 is located below the protruding driving arm 66 of the magnetic rod bearing block 64, and the magnetic rod bearing block 64 is lifted by the driving arm 66 to be lifted upwards. Furthermore, as shown in fig. 3, the magnetic sleeve driving block 74 and the magnetic rod bearing block 64 are both sleeved on the first guiding shaft 81, and the magnetic sleeve driving block 74 is located below the magnetic rod bearing block 64; the magnetic rod driving block 65 is arranged on one side of the magnetic rod bearing block 64 and the magnetic sleeve driving block 74 far away from the magnetic rod 62 and the magnetic sleeve; the driving arm 66 on the magnetic rod bearing block 64 is arranged to protrude towards the side where the magnetic rod driving block 65 is located; the magnetic rod driving block 65 is located below the driving arm 66, and a space is formed between one side wall of the magnetic rod driving block 65 close to the magnetic rod bearing block 64 and the magnetic sleeve driving block 74 in the horizontal direction, so as to avoid the magnetic rod driving block 65 interfering with and colliding with the magnetic sleeve driving block 74 when moving up and down.

Preferably, the base assembly is provided with two second guide shafts 82, the two second guide shafts 82 are symmetrically arranged on two sides of the first lead screw, and correspondingly, the magnetic rod driving block 65 is provided with two third guide holes which are respectively sleeved on the two second guide shafts 82 to play a role in guiding and improve the movement stability and reliability of the magnetic rod driving block 65.

In the arrangement mode of the magnetic rod bearing block 64, the magnetic rod driving block 65 and the magnetic sleeve driving block 74, the magnetic sleeve driving block 74 is directly connected with the second driving assembly, and the second driving assembly directly drives the magnetic sleeve driving structure to move up and down along the vertical direction; the magnetic rod driving block 65 is positioned below the magnetic rod bearing block 64, the magnetic rod driving block 65 is directly connected with the first driving component, and the upper surface of the magnetic rod driving block 65 is in contact connection with the lower surface of the magnetic rod bearing block 64, namely only the surface is in contact, and the magnetic rod driving block is not directly connected through a mechanical structure; the first driving assembly drives the magnetic rod driving block 65 to move upwards, indirectly supports the magnetic rod bearing block 64 to move upwards, and the magnetic rod bearing block 64 moves downwards by means of the self-gravity of the magnetic rod assembly 6.

By adopting the above arrangement, when the magnetic rod 62 is required to move upwards, the magnetic rod driving block 65 can be driven to move upwards, and the magnetic rod rack 61 and the magnetic rod 62 are further supported; when the magnetic rod 62 needs to move downwards, the magnetic rod driving block 65 moves downwards, and the magnetic rod frame 61 and the magnetic rod 62 move downwards by means of gravity, so that even if the motion precision of the magnetic rod driving block 65 is in error or the motion control fails, the magnetic rod driving block 65 moves downwards excessively, the magnetic rod frame 61 can only be placed on the magnetic sleeve frame 71, excessive pressure cannot be applied to the magnetic sleeve frame 71, the magnetic rod 62 cannot excessively abut against the magnetic sleeve, and the problem of instrument damage is avoided; when the magnetic rod 62 and the magnetic rod sleeve need to be lifted together, the magnetic sleeve driving block 74 can be driven to move up and down only, and the magnetic rod arm 63 is driven to move up and down by the magnetic sleeve arm 73, so that the requirement on the matching precision of the first motor and the second motor is reduced, and the complexity of instrument hardware and software is simplified; meanwhile, the magnetic rod assembly is driven to move by adopting a direct driving mode, the overall motion stability of the driving assembly 4 can be ensured as much as possible on the premise of ensuring the moving safety of the magnetic rod and the magnetic rod sleeve, and the overall motion precision of the driving assembly 4 is improved. In addition, the magnetic rod bearing block 64, the magnetic rod driving block 65 and the magnetic sleeve driving block 74 are arranged in the arrangement mode, so that the transmission structure of the instrument is compactly arranged together, and the volume of the instrument is reduced.

Further, as shown in fig. 3 to 5, the third driving assembly includes a third motor and a third lead screw, the third motor is disposed in the accommodating space of the base assembly, and the third lead screw is disposed in the accommodating space along the horizontal direction and is rotatably connected to the third motor. A bracket 15 is arranged in a gap formed between two side supports 14 of the base assembly, the bracket 15 is fixedly arranged on an instrument bottom plate, one end of a third lead screw is connected with a side support plate 13 of the base assembly through a threaded structure, and the other end of the third lead screw is connected with the bracket 15. When the third motor drive third lead screw rotated, the base subassembly was whole can to follow the third lead screw and remove about, and then drives bar magnet 62 and bar magnet cover and remove about to aim at different kit test tubes.

By combining the description of the first driving assembly, the second driving assembly and the third driving assembly, the first motor, the second motor and the third motor are all arranged in the accommodating space of the base assembly, the first lead screw and the second lead screw penetrate through the upper base plate 11 and extend upwards above the upper base plate 11, the magnetic rod assembly 6 and the magnetic sleeve assembly 7 are arranged on the first lead screw and the second lead screw, and the first guide shaft 81 and the second guide shaft 82 are directly and fixedly connected to the upper base plate 11 and are respectively parallel to the first lead screw and the second lead screw and arranged side by side.

Further, as shown in fig. 6 to 11, the magnetic bar frame 61 is a rectangular frame, the first hollow structure is disposed at the central portion of the magnetic bar frame 61, and the magnetic bars 62 are divided into two rows and symmetrically disposed at two sides of the first hollow structure. The bar magnet arm 63 connected with the bar magnet frame 61 is L-shaped, and comprises a first connecting arm arranged along the transverse direction and a second connecting arm arranged along the vertical direction, wherein the first connecting arm is fixedly connected with the bar magnet frame 61, and the second connecting arm is fixedly connected with the bar magnet bearing block 64. The magnetic bar bearing block 64 is a rectangular block-shaped structure, a first side face is connected with the second fixing arm, and a convex driving arm 66 is arranged at a second side face opposite to the first side face. The magnetic bar bearing block 64 is provided with two second guide holes matched with the first guide shaft 81, and the central position of the magnetic bar bearing block 64 is also provided with a yielding hole 67.

The outline shape of the magnetic sleeve frame 71 is similar to that of the magnetic bar frame 61, two second hollow structures which are symmetrically arranged are arranged on the magnetic sleeve frame 71, and the positions of the second hollow structures correspond to the positions of the magnetic bars 62 on the magnetic bar frame 61; the lower surface of the magnetic sleeve frame 71 is also provided with two sliding grooves 72, the sliding grooves 72 are opposite to the second hollow structure, the magnetic rod sleeve can be clamped on the lower surface of the magnetic sleeve frame 71 in a sliding installation mode and is detached and replaced when nucleic acid extraction is carried out each time; a third hollow structure is further arranged between the two second hollow structures, and the position of the third hollow structure corresponds to the position of the first hollow structure on the magnetic rod frame 61. The magnetic sleeve arm 73 connected with the magnetic sleeve frame 71 is also L-shaped and comprises a third connecting arm arranged along the transverse direction and a fourth connecting arm arranged along the vertical direction, the third connecting arm is fixedly connected with the magnetic sleeve frame 71, and the fourth connecting arm is fixedly connected with the magnetic sleeve driving block 74. The magnetic sleeve driving block 74 is provided with two first guiding holes matching with the first guiding shaft 81, and the center of the magnetic sleeve driving block 74 is further provided with a driving hole 75.

When the magnetic rod assembly 6 and the magnetic sleeve assembly 7 are stacked together, the first connecting arm of the magnetic rod arm 63 and the third connecting arm of the magnetic sleeve arm 73 are oppositely arranged, and the magnetic rod 62 penetrates through the second hollow structure of the magnetic sleeve frame 71 and is embedded into the magnetic rod sleeve; the first hollow structure on the magnetic rod frame 61 and the third hollow structure on the magnetic sleeve frame 71 are overlapped with each other. In addition, because bar magnet carrier block 64 sets up directly over magnetic sleeve drive block 74, in order to avoid bar magnet carrier block 64 and second lead screw to take place position interference, set up hole 67 of stepping down on bar magnet carrier block 64, hole 67 of stepping down corresponds the setting with drive hole 75 on the magnetic sleeve drive block 74, the second lead screw passes drive hole 75 in proper order and the setting of hole 67 of stepping down, be connected through helicitic texture with drive hole 75, and then realize the transmission, set up with the inner wall mutual interval of hole 67 of stepping down, do not contact, and then avoid mutual interference.

Further, the driving assembly 4 of the nucleic acid extractor of the present invention further includes an infrared detection assembly, specifically as shown in fig. 1, fig. 4 and fig. 5, including a first photoelectric blocking piece 91, a second photoelectric blocking piece 92 and a third photoelectric blocking piece 93, where the first photoelectric blocking piece 91 can detect a left-right movement position of the base assembly in the horizontal direction, the second photoelectric blocking piece 92 can detect a movement position of the magnetic sleeve driving block 74 in the vertical direction, and the third photoelectric blocking piece 93 can detect a movement position of the magnetic rod driving block 65 in the vertical direction.

The first photoelectric blocking piece 91 is a long-strip sheet-shaped structure, is arranged on the lower bottom plate 12 of the base assembly, and is positioned on one side of the lower bottom plate 12, which is far away from the magnetic rod assembly 6 and the magnetic sleeve assembly 7; two first sensors 94 are further disposed at positions close to the first photoelectric barrier 91, and the two first sensors 94 are fixed on the instrument base plate and are respectively disposed close to two ends of the first photoelectric barrier 91. The two first sensors 94 can respectively sense the moving positions of the two ends of the first photoelectric barrier 91, and when the end of the first photoelectric barrier 91 is detected to exceed the position of the first sensor 94, the instrument can take measures such as alarming and sudden stop, so that the situation that the driving assembly 4 of the instrument moves beyond the maximum value in the transverse direction and the collision of components occurs is avoided.

The second photoelectric blocking piece 92 is arranged on the side wall of the magnetic sleeve driving block 74, and is matched with the magnetic sleeve driving block, two second sensors 95 are also arranged at the position close to the second photoelectric blocking piece 92, and the two second sensors 95 are vertically arranged and fixedly connected to the inner wall of the side plate of the driving assembly 4; two second sensors 95 can respond to the shift position of two tip of second photoelectric separation blade 92 respectively, and when detecting that the tip of second photoelectric separation blade 92 surpassed second sensor 95 position, measures such as warning, scram can be taken to the instrument, avoids magnetic sleeve subassembly 7 to move in vertical direction and surpasss the maximum value, takes place the part collision.

The third photoelectric blocking piece 93 is arranged on the side wall of the magnetic rod driving block 65, and is matched with the magnetic rod driving block, two third sensors 96 are also arranged at the position close to the third photoelectric blocking piece 93, and the two third sensors 96 are vertically arranged and fixedly connected to the inner wall of the side plate of the driving assembly 4; the two third sensors 96 can respectively sense the moving positions of the two ends of the third photoelectric blocking piece 93, when the end of the third photoelectric blocking piece 93 is detected to exceed the position of the third sensor 96, the instrument can take measures such as alarming and sudden stop, and the phenomenon that the magnetic rod assembly 6 moves in the vertical direction to exceed the maximum value and the collision of components is avoided.

The infrared detection component on the driving component 4 of the nucleic acid extractor can further detect the moving position of each component in the driving component 4, control the moving range of the components and further ensure the motion safety of the driving component 4.

Further, a heating component is arranged at the reagent bin module 5 and used for heating a cracking station and an elution station in the reagent box. Specifically, the heating assembly comprises a base 100, wherein a first heating body 200 and a second heating body 300 are arranged on the base 100 and respectively correspond to the cracking station and the elution station of the kit. Wherein, a first heating surface is formed on the first heating body 200, and the first heating surface is arranged close to the cracking station test tube of the kit and is used for heating the liquid in the cracking station test tube; a second heating surface is formed on the second heating body 300, is arranged close to the elution station test tube of the kit and is used for heating liquid in the elution station test tube; the height of the first heating surface is greater than that of the second heating surface.

According to different requirements of users on sample extraction quantity, one, two or more groups of first heating bodies 200 and second heating bodies 300 are selectively arranged on the base 100 and used for heating one, two or more groups of cracking stations and elution stations.

Specifically, as shown in fig. 12 to 16, the base 100 includes a supporting plate 110 disposed along a horizontal direction, two or more mounting grooves 130 are formed on the supporting plate 110, and the first heating body 200 and the second heating body 300 are respectively embedded in the different mounting grooves 130 to be fixedly connected with the base 100. The edge of the supporting plate 110 is formed with a boss 120, and the boss 120 extends from the upper surface of the supporting plate 110 along the direction away from the upper surface, so as to form a containing bin on the supporting platform, wherein the containing bin is used for containing a reagent kit containing a nucleic acid extraction reagent. Preferably, the bosses 120 are formed at both side edge portions of the support plate 110 and at an edge portion of the support plate 110 on a side close to the driving unit, and an opening is formed at a side of the support plate 110 corresponding to the nucleic acid extracting instrument door 2 to facilitate the insertion or removal of the reagent cartridge.

Four pillars 400 are provided at the bottom of the base 100, and are respectively provided at four corners of the base 100, one end of the pillar 400 is fixedly connected to the base 100, and the other end is fixedly connected to the instrument base plate of the nucleic acid extracting instrument. The pillar 400 is used to fixedly dispose the base 100 on the instrument bottom plate, on the one hand, and the pillar 400 supports the base 100 such that a space is formed between the base 100 and the instrument bottom plate, on the other hand; the fan 500 is arranged in the space, the fan 500 is fixedly connected to the instrument base plate, one side of the fan 500 is arranged corresponding to the vent hole on the instrument base plate, and the other side is arranged corresponding to the first heating body 200 and the second heating body 300, so as to ventilate and cool the first heating body 200 and the second heating body 300.

Further, as shown in fig. 17 and 18, the first heating body 200 and the second heating body 300 are both strip-shaped block-shaped structures, and are preferably made of a material having good heat conductivity, such as metal. The first heating body 200 includes a first heat generating portion 210 and a first heat supplying portion 220, and the first heat generating portion 210 and the first heat supplying portion 220 are preferably integrally formed. A first through groove 230 is formed in the first heat generating part 210, and the first through groove 230 extends along the length direction of the first heating body 200; the first heat supplying portion 220 protrudes from the first heat generating portion 210, and a plurality of first heating grooves 240 are formed on the first heat supplying portion 220. In the first through groove 230 of the first heating portion 210, a heating element (not shown in the figure) is disposed, when the lysis step of nucleic acid extraction is performed, the test tube at the lysis station on the reagent kit is located in the first heating groove 240, the heating element can generate heat and transfer the heat to the first heating portion 210, and the first heating portion 210 transfers the heat to the first heat supplying portion 220 to heat the test tube embedded in the first heating groove 240 and the liquid in the test tube.

Similarly, second heating body 300 includes second heat generating portion 310 and second heat supplying portion 320, and second heat generating portion 310 and second heat supplying portion 320 are preferably integrally formed. A second through groove 330 is formed in the second heat generating portion 310, and the second through groove 330 extends along the length direction of the second heating body 300; the second heat supplying portion 320 protrudes from the second heat generating portion 310, and a plurality of second heat grooves 340 are formed on the second heat supplying portion 320. In the second through groove 330 of the second heating portion 310, a heating element (not shown) is disposed, when the elution step of nucleic acid extraction is performed, the test tube at the elution station on the reagent kit is located in the second heating groove 340, the heating element can generate heat and transfer the heat to the second heating portion 310, and the second heating portion 310 transfers the heat to the second heating portion 320, so as to heat the test tube embedded in the second heating groove 340 and the liquid in the test tube.

Further, the depth of the first heating groove 240 of the first heating body 200 is greater than the depth of the second heating groove 340 of the second heating body 300. Specifically, when nucleic acid extraction is performed, the amount of the reagent required to be added into the test tube in the lysis step is large, and the amount of the reagent required to be added into the test tube in the elution step is small, so that the liquid level in the lysis test tube is high, and the liquid level in the elution test tube is low; in order to achieve better heating for both the lysis and elution steps, it is preferable that the depth of the first heating bath 240 matches the liquid level in the lysis tube and the depth of the second heating bath 340 matches the liquid level in the elution tube. Then, place on base 100 when the kit to when setting up with first heating body 200 and the cooperation of second heating body 300, the test tube embedding of schizolysis station is to the degree of depth in first heating groove 240, is greater than the degree of depth of the test tube embedding of elution station to second heating groove 340, and the liquid level height of schizolysis station test tube and the degree of depth phase-match of first heating groove 240, the liquid level height of elution station test tube and the degree of depth phase-match of second heating groove 340.

Specifically, as shown in fig. 16, 20 and 21, the mounting groove 130 on the base 100 includes a first groove body and a second groove body, the first groove body is located above the second groove body, and the width of the first groove body is smaller than that of the second groove body, so that a boss structure is formed at a position where the first groove body is connected with the second groove body; the width of the first heat supplying portion 220 of the first heating body 200 is smaller than that of the first heat generating portion 210, so that a boss structure is formed at the junction of the first heat supplying portion 220 and the first heat generating portion 210. When the first heating body 200 is installed, the first heating body 200 is inserted into the installation groove 130 from the lower side of the base 100, so that the first heat supply part 220 sequentially passes through the second tank body and the first tank body and extends out of the upper surface of the base 100; the boss structure on the first heating body 200 and the boss structure in the mounting groove 130 are abutted against each other to limit the position of the first heating body 200 in the mounting groove 130 and fix the height of the first heat supply part 220 extending out of the upper surface of the base 100; the fixing component is arranged below the base 100, the fixing component is provided with a clamping groove, a part of the structure of the first heating part 210 protrudes out of the lower surface of the base 100, and the clamping groove on the fixing component can be clamped on the protruding part of the first heating part 210 so as to fix the first heating part 210 below the base 100, and further, the first heating body 200 is stable and immovable in the mounting groove 130.

Similarly, the second heating body 300 is installed in the installation groove 130 of the base 100 in the same manner. The second heating body 300 is inserted into the mounting groove 130 from the lower side of the base 100, so that the second heat supplying part 320 sequentially passes through the second groove body and the first groove body and extends out of the upper surface of the base 100; the boss structure on the second heating body 300 and the boss structure in the mounting groove 130 are abutted against each other to limit the position of the second heating body 300 in the mounting groove 130 and fix the height of the second heat supply part 320 extending out of the upper surface of the base 100; the second heat generating part 310 protrudes from the lower surface of the base 100, and the fixing component below the base 100 can fix the second heat generating part 310 below the base 100 through the locking slot, so that the second heating body 300 is stable in the mounting slot 130.

Preferably, the length of the first heat supply portion 220 of the first heating body 200 is greater than the length of the second heat supply portion 320 of the second heating body 300, so that the height of the first heat supply portion 220 extending to the upper surface of the base 100 is higher than the height of the second heat supply portion 320 extending to the upper surface of the base 100; the first heating groove 240 is formed at a portion where the first heat supplying part 220 extends to the upper surface of the base 100, and the second heating groove 340 is formed at a portion where the second heat supplying part 320 extends to the upper surface of the base 100, so that the depth of the first heating groove 240 is greater than that of the second heating groove 340.

Of course, besides the above structure, other structural forms can be adopted to set the first heating body 200 and the second heating body 300, so that the height of the contact surface of the first heating body 200 and the lysis test tube is greater than the height of the contact surface of the second heating body 300 and the elution test tube. For example, carry out the spiral winding with the metal wire of easy heat conduction, form the heating member of annular, wherein, increase with the corresponding heating member winding number of turns of schizolysis test tube, the loop configuration that makes the formation is longer in vertical direction length, and then the height of increase and schizolysis test tube contact surface, and the corresponding heating member winding number of turns with the elution test tube reduces, and the loop configuration that makes the formation is shorter in vertical direction length, and then reduces the height with elution test tube contact surface.

The first heating body 200 and the second heating body 300 arranged in the above manner have the following beneficial effects:

(1) the liquid amount in the cracking test tube is large, so that the temperature can meet the cracking requirement only by acquiring more heat, and the depth of the first heating groove 240 is deep, so that the heat transfer area between the first heating body 200 and the cracking test tube can be increased, the heating efficiency is improved, the liquid in the cracking test tube is rapidly heated, and the overall speed of nucleic acid extraction is improved;

(2) the cracking station can quickly finish the heating and cracking steps, and also can greatly reduce heat radiation caused by overlong heating time, so that the phenomenon that the heat at the cracking station is excessively radiated to other stations nearby for washing, elution and the like to influence the working effects of other steps is prevented;

(3) the amount of liquid in the elution test tube is small, once the heating amount is too large, the elution liquid can be excessively volatilized, reagents are wasted, and the concentration of the reagents can be influenced;

(4) the degree of depth of second heating tank 340 and the liquid level height phase-match in the elution test tube are not higher than the liquid level height of eluant, can avoid in the oscillation process, and when the magnetic bead was oscillated more than the liquid level of eluant, the problem of the magnetic bead evaporation dry junction that is heated takes place on the inner wall of test tube, and then guarantees that the nucleic acid on the magnetic bead can be dissolved in the eluant completely, does not influence sample quality.

Through the matching arrangement mode of the first heating body 200 and the second heating body 300, the heating assembly has quicker and better heating effect, and the efficiency and the extraction quality of nucleic acid extraction are improved.

Further, as shown in fig. 20 and 21, a heat insulation block 600 is disposed between the first and

second heating bodies

200 and 300 and the mounting groove 130 of the base 100, and the heat insulation block 600 is made of a material that is resistant to high temperature and not easy to conduct heat, so as to isolate the first and

second heating bodies

200 and 300 from the base 100. The heat insulation block 600 can protect the base 100 from high temperature damage, and can reduce heat conduction of the first heating body 200 and the second heating body 300 to the base 100, thereby reducing heat loss.

Further, as shown in fig. 16, 10 and 21, the fixing assembly for fixing the first and

second heating bodies

200 and 300 includes a first fixing clip 700 and a second fixing clip 800, and the first fixing clip 700 and the second fixing clip 800 are respectively located at two end positions of the first and

second heating bodies

200 and 300 to fixedly connect two ends of the first and

second heating bodies

200 and 300 to the base 100.

Specifically, the first fixing clip 700 and the second fixing clip 800 are both strip-shaped block structures, two or more first clamping grooves 710 are arranged on the first fixing clip 700, and when the first fixing clip 700 is fixedly connected with the base 100, the first clamping grooves 710 are clamped at the lower parts of the first heating body 200 and the second heating body 300 so as to fix the first heating body 200 and the second heating body 300; similarly, the second fixing clip 800 is provided with two or more second fastening grooves 810, and when the second fixing clip 800 is fixedly connected to the base 100, the second fastening grooves 810 are fastened to the lower portions of the first heating body 200 and the second heating body 300 to fasten the first heating body 200 and the second heating body 300. The number and the open positions of the first and second engaging

grooves

710 and 810 correspond to the number and the positions of the first and

second heating bodies

200 and 300.

Adopt above-mentioned mounting groove 130 and fixing clip matched with fixed mode, combine the fixed knot structure of first heating member 200 and second heating member 300, can be stably with first heating member 200 and the fixed setting on base 100 of second heating member 300, and the assembly precision of heating member is high, difficult emergence offset or dislocation from top to bottom are favorable to improving kit and heating device's cooperation precision, guarantee nucleic acid extraction's quality and efficiency.

As shown in fig. 19 and 21, a concave portion is further disposed in the first engaging groove 710 of the first fixing clip 700, and when the first fixing clip 700 fixes the first heating body 200 and the second heating body 300 and is fixedly connected to the lower surface of the base 100, the concave portion is disposed close to and communicated with the first heating body 200 or the second heating body 300. The concave part is provided with a heat preservation device (not shown in the figure), the heat preservation device is electrically connected with the heating element in the first heating body 200 and/or the second heating body 300, and the heating element can be controlled to be powered on or powered off, so as to achieve the function of temperature protection. The temperature-keeping device is provided with a temperature sensor and an upper temperature limit value, when the instrument or the heating device fails to work and the temperature control function of the instrument fails to work, the temperature-keeping device senses that the temperature of the first heating body 200 and/or the second heating body 300 exceeds the upper temperature limit value, the temperature-keeping device can enable the heating element to be in an open circuit, and accidents or instrument damage caused by continuous heating of the heating element are avoided; when the temperature-keeping device senses that the temperature of the first heating body 200 and/or the second heating body 300 is reduced below the upper temperature limit value, the heating element is communicated again to heat the heating element.

As shown in fig. 17 and 18, the side walls of the first heating body 200 and the second heating body 300 are provided with a temperature measuring hole 910 and a temperature measuring channel 920, and the temperature measuring hole 910 is communicated with the temperature measuring channel 920; the temperature measuring holes 910 are provided with temperature measuring probes, and the wiring of the temperature measuring probes is arranged along the temperature measuring channel 920, so as to realize real-time detection of the temperatures of the first heating body 200 and the second heating body 300. The temperature data detected by the temperature probe can be used by the temperature control device of the instrument and/or the temperature protection device.

Preferably, the temperature measuring hole 910 is disposed at a middle position of the sidewalls of the first heating body 200 and the second heating body 300, near a position where the first heat supplying part 220 of the first heating body 200 and the first heat generating part 210 are connected, or near a position where the second heat supplying part 320 of the second heating body 300 and the second heat generating part 310 are connected. The temperature measuring channel 920 is opened on the sidewall of the first heating body 210 or the second heating body 310. On one hand, the temperature measuring holes 910 are arranged in the central areas of the first heating body 200 and the second heating body 300, which is beneficial to improving the accuracy and reliability of the temperature detected by the temperature measuring probe; on the other hand, when the first heating body 200 and the second heating body 300 are installed on the base 100, the temperature measuring channel 920 can be hidden inside and under the base 100, and is not exposed out of the upper surface of the base 100, which is not only beautiful, but also convenient for wiring.

Further, as shown in fig. 17 and 18, a plurality of first hollow structures are formed on the first heat supplying portion 220 of the first heating body 200, and the first hollow structures are disposed between two adjacent first heating grooves 240, so that the two adjacent first heating grooves 240 are communicated with each other. A plurality of second hollow structures are further formed on the first heat supply portion 220 of the first heating body 200, the second hollow structures are arranged on the side walls of the two sides of the first heat supply portion 220, and two second hollow structures are symmetrically arranged on two sides of each first heating groove 240. When the kit and the cooperation of first heating member 200 set up, first heat supply portion 220 cover is established in the outside of schizolysis test tube, because the height of first heat supply portion 220 is higher, therefore first heat supply portion 220 can extend to the liquid level place high position of schizolysis test tube. And the test tube on the kit is more in quantity, arranges densely, and the interval is less between the test tube of adjacent position, therefore the test tube is likely to take place mutual interference between first heat supply portion 220. The first hollow structure is arranged to avoid mutual interference between two cracking test tubes located at adjacent positions and the first heat supply part 220, and the second hollow structure is arranged to avoid mutual interference between the test tubes of one column of cracking stations and the test tubes of two adjacent columns of other stations due to the first heat supply part 220; in addition, first hollow out construction can also promote the heat to flow between a plurality of first heating tanks 240, makes a plurality of schizolysis test tubes thermally equivalent.

Further, as shown in fig. 17 and 18, a plurality of heating columns 250 are formed on the first heat supplying part 220 of the first heating body 200, and the plurality of heating columns 250 are arranged side by side to constitute a plurality of first heating grooves 240. Heating column 250 is similar to the triangular prism shape, is located between two adjacent schizolysis test tubes, and the shape phase-match in the gap that forms between two adjacent schizolysis test tubes, can press close to the pipe wall of test tube as far as possible on the one hand, promotes heat transfer, can fill the gap that forms between two schizolysis test tubes completely on the one hand, and increase calorific capacity, and then improve the rate of heating of first heating member 200.

Further, as shown in fig. 15 and 21, the first heating body 200 and the second heating body 300 are spaced apart to further reduce mutual radiation of heat, thereby avoiding affecting the concentration of the reagent and the extraction effect of nucleic acid. Preferably, the first heating body 200 and the second heating body 300 are spaced apart by at least the length of a row of test tubes on the reagent cartridge.

The present invention has been further described with reference to specific embodiments, but it should be understood that the detailed description should not be construed as limiting the spirit and scope of the present invention, and various modifications made to the above-described embodiments by those of ordinary skill in the art after reading this specification are within the scope of the present invention.

Claims

1. A nucleic acid extraction instrument comprises a driving assembly and a reagent bin module, and is characterized in that the driving assembly comprises a magnetic rod assembly and a magnetic sleeve assembly, the magnetic rod assembly and the magnetic sleeve assembly are arranged above the reagent bin module, the magnetic sleeve assembly comprises a magnetic rod sleeve, a magnetic sleeve driving structure is connected onto the magnetic rod sleeve, the magnetic sleeve driving structure is directly connected with a second driving assembly, and the second driving assembly directly drives the magnetic sleeve driving structure to move; the bar magnet subassembly includes the bar magnet, be connected with bar magnet bearing structure on the bar magnet, the extrusion is provided with the actuating arm on the bar magnet bearing structure, the below of actuating arm is provided with bar magnet drive structure, bar magnet drive structure and first drive assembly lug connection, the surface of bar magnet drive structure is connected for the contact with the surface of actuating arm, first drive assembly drive bar magnet drive structure rebound, the indirect actuating arm that supports drives the bar magnet bearing structure and shifts up, bar magnet bearing structure relies on bar magnet subassembly self gravity downstream.

2. The nucleic acid extractor of claim 1, wherein the magnetic rod is fixedly connected to a magnetic rod holder, the magnetic rod sleeve is detachably disposed on the magnetic rod sleeve holder, the magnetic rod bearing structure comprises a magnetic rod arm and a magnetic rod bearing block, the magnetic rod arm comprises a first connecting arm disposed along a transverse direction and a second connecting arm disposed along a vertical direction, the first connecting arm is fixedly connected to the magnetic rod holder, and the second connecting arm is fixedly connected to the magnetic rod bearing block; the magnetic sleeve driving structure comprises a magnetic sleeve arm and a magnetic sleeve driving block, the magnetic sleeve arm comprises a third connecting arm arranged along the transverse direction and a fourth connecting arm arranged along the vertical direction, the third connecting arm is fixedly connected with the magnetic sleeve frame, and the fourth connecting arm is fixedly connected with the magnetic sleeve driving block; the first connecting arm and the third connecting arm are oppositely arranged.

3. The nucleic acid extractor of claim 1, wherein the first driving assembly comprises a first motor and a first lead screw, the second driving assembly comprises a second motor and a second lead screw, the first motor and the second motor are fixedly disposed on the base assembly, one end of the first lead screw is connected to the first motor, the other end of the first lead screw extends in a direction away from the base assembly along the vertical direction, one end of the second lead screw is connected to the second motor, and the other end of the second lead screw extends in a direction away from the base assembly along the vertical direction; the base assembly is movably connected with the instrument bottom plate, a third lead screw is connected onto the base assembly, the third lead screw extends along the transverse direction, a third motor is connected onto the third lead screw, and the third motor can drive the third lead screw to drive the base assembly to move on the instrument bottom plate.

4. The nucleic acid extraction apparatus of claim 3, wherein the base assembly comprises an upper plate and a lower plate, the upper plate is disposed above the lower plate, a side support plate and two side supports are disposed between the upper plate and the lower plate, the side support plate connects the first ends of the upper plate and the lower plate, the two side supports connect the second ends of the upper plate and the lower plate, an accommodation space is formed between the upper plate and the lower plate, the first motor, the second motor and the third motor are disposed in the accommodation space, the first lead screw and the second lead screw penetrate through the upper base plate and extend upwards, the magnetic bar driving structure is arranged on the first lead screw, the magnetic sleeve driving structure is arranged on the second lead screw, a gap is formed between the two side supports, a support is arranged in the gap, the support is fixedly arranged on the instrument base plate, one end of the third lead screw is connected with the support plate, and the other end of the third lead screw is connected with the support.

5. The nucleic acid extractor as claimed in claim 4, wherein the base assembly is provided with a first guide shaft and a second guide shaft, the first guide shaft is parallel to and arranged side by side with the first lead screw, one end of the first guide shaft is fixedly connected with the upper base plate, the other end of the first guide shaft extends in a vertical direction towards the upper side of the base assembly, the magnetic rod bearing structure is provided with a second guide hole, the second guide hole is sleeved on the first guide shaft, and the magnetic rod bearing structure can drive the magnetic rod to slide up and down along the first guide shaft; the magnetic sleeve driving structure is provided with a first guide hole, the first guide hole is sleeved on a first guide shaft, so that a guiding effect is achieved on the magnetic sleeve driving structure, one end of a second guide shaft is fixedly connected with an upper base plate, the other end of the second guide shaft extends towards the upper portion of a base assembly along the vertical direction, the second guide shaft is parallel to a second lead screw and is arranged side by side, a third guide hole is formed in the magnetic rod driving structure, and the third guide hole is sleeved on the second guide shaft and achieves a guiding effect on the magnetic rod driving structure.

6. The nucleic acid extractor according to any of claims 3 to 5, comprising an infrared detection assembly, wherein the infrared detection assembly comprises a first photoelectric barrier, a second photoelectric barrier, a third photoelectric barrier, and a sensor disposed in cooperation with the first photoelectric barrier, the second photoelectric barrier, and the third photoelectric barrier, the first photoelectric barrier is disposed on the base assembly and can move along with the base assembly in the horizontal direction, the second photoelectric barrier is disposed on the magnetic sleeve driving structure and can move up and down along with the magnetic sleeve driving structure, and the third photoelectric barrier is disposed on the magnetic rod driving structure and can move up and down along with the magnetic rod driving structure.

7. The nucleic acid extraction instrument according to any one of claims 1 to 5, wherein the reagent chamber module comprises a heating assembly, the heating assembly comprises a base for supporting the nucleic acid extraction reagent kit, a first heating body and a second heating body are arranged on the base, the first heating body is arranged corresponding to the cracking station test tube of the reagent kit, the second heating body is arranged corresponding to the elution station test tube of the reagent kit, a first heating groove is formed on the first heating body, a second heating groove is formed on the second heating body, the depth of the first heating groove is greater than that of the second heating groove, the depth of the first heating groove matches with the liquid level in the cracking station test tube, and the depth of the second heating groove matches with the liquid level in the elution station test tube.

8. The nucleic acid isolation instrument according to claim 7, wherein the first heating body includes a first heat supply portion and a first heat generation portion which are connected, the second heating body includes a second heat supply portion and a second heat generation portion which are connected, the plurality of first heating grooves are formed in the first heat supply portion, the plurality of second heating grooves are formed in the second heat supply portion, the length of the first heat supply portion is longer than that of the second heat supply portion, a first through groove is formed in the first heat generation portion, a second through groove is formed in the second heat generation portion, and heat generation elements are provided in the first through groove and the second through groove.

9. The nucleic acid extraction instrument according to claim 8, wherein the first heat supply portion has a plurality of first hollow structures and a plurality of second hollow structures formed thereon, the first hollow structures are disposed between two adjacent first heat grooves, and the second hollow structures are disposed on two side walls of the first heat supply portion and symmetrically disposed on two sides of each first heat groove; be formed with a plurality of heating posts in the first heat supply portion, the heating post is located between two adjacent schizolysis station test tubes, the shape phase-match in the gap that forms between the shape of heating post and two adjacent schizolysis test tubes.

10. The nucleic acid extractor according to claim 8, wherein the side walls of the first heating body and the second heating body are provided with a temperature measuring hole and a temperature measuring channel, a temperature measuring probe is arranged in the temperature measuring hole, the temperature measuring channel is communicated with the temperature measuring hole, and the wiring of the temperature measuring probe is arranged along the temperature measuring channel; the base is provided with at least two mounting grooves, the first heating body and the second heating body are arranged in different mounting grooves, a fixing clamp is arranged below the base, a clamping groove is formed in the fixing clamp, and the clamping groove is clamped below the first heating part and the second heating part and is fixedly connected with the lower surface of the base; a lower concave part is arranged in the clamping groove, a heat preservation device is arranged in the lower concave part, and the heat preservation device is electrically connected with the heating element in the first heating body and/or the second heating body so as to control the heating element to be powered on or powered off.