CN217614815U

CN217614815U - Imbibition drain of hybridization apparatus

Info

Publication number: CN217614815U
Application number: CN202221701458.6U
Authority: CN
Inventors: 孙悦; 陈立波
Original assignee: Fist Shanghai Biotechnology Co ltd
Current assignee: Fist Shanghai Biotechnology Co ltd
Priority date: 2022-05-24
Filing date: 2022-07-01
Publication date: 2022-10-21
Anticipated expiration: 2032-07-01
Also published as: CN217479439U; CN217628334U; CN116410843A; CN217479473U; CN217479466U

Abstract

The utility model provides a imbibition flowing back device of hybridization appearance, a serial communication port, hybridization appearance includes the casing, is equipped with hybridization portion and imbibition flowing back device in the casing, and hybridization portion is equipped with at least one reactor, is equipped with biochip in the reactor, imbibition flowing back device is including flourishing liquid portion, transmission and drive division, and flourishing liquid portion has held liquid, and the transmission is connected to the one end of reactor and flourishing liquid portion intercommunication, the other end, and the drive division is connected and drives the transmission, and the transmission exerts pressure in to the reactor, makes the liquid of flourishing liquid portion be inhaled in the reactor or with the liquid discharge in the reactor. The utility model discloses reaction unit detachable installs in the hybridization appearance, through imbibition flowing back device inhales reaction unit with liquid or with the liquid discharge in the reaction unit, after the reaction is accomplished with reaction unit demolish can, liquid can not pollute the hybridization appearance body, need not frequent washing with the degree of depth, has improved the performance and the life-span of hybridization appearance.

Description

Imbibition drain of hybridization appearance

Technical Field

The utility model belongs to the technical field of the hybridization appearance, specifically be an imbibition flowing back device of hybridization appearance.

Background

The hybridization instrument is an analytical instrument which is generally used in the biological field, and adopts the nucleic acid molecular hybridization technology to detect whether the genome to be detected contains a known gene sequence. When the kit is used, the liquid (hybridization liquid, binding liquid, sample liquid, etc.) and the biochip are hybridized, and then necessary reaction conditions such as temperature are provided, and after the reaction is completed, the detection result can be judged according to the color and state of the reacted reactant. In this process, it is necessary to introduce a liquid into the hybridization apparatus and to clean the hybridization apparatus after use, which reduces the efficiency, performance and life of the hybridization apparatus.

SUMMERY OF THE UTILITY MODEL

To the above-mentioned problem that prior art exists, the utility model aims at providing an imbibition drain of hybridization instrument, reaction unit detachable installs in the hybridization instrument, the reaction of a plurality of reaction units can be accomplished once to the hybridization instrument, through imbibition drain inhales liquid reaction unit or with the liquid discharge in the reaction unit, after the reaction is accomplished with reaction unit demolish can, make the transfer of liquid controllable, liquid can not pollute the hybridization instrument body, need not frequent washing with the degree of depth, improved the performance and the life-span of hybridization instrument.

In order to realize the purpose, the utility model discloses the technical scheme who adopts is:

the utility model provides a imbibition drain of hybridization appearance, hybridization appearance includes the casing, is equipped with hybridization portion and imbibition drain in the casing, and hybridization portion is equipped with at least a set of reaction assembly, and reaction assembly includes a reactor, is equipped with biochip in the reactor, imbibition drain is including flourishing liquid portion, transmission and drive division, and flourishing liquid portion has held liquid, and the transmission is connected to the one end of reactor and flourishing liquid portion intercommunication, the other end, and drive division connects and drives transmission, and transmission exerts pressure in to the reactor, makes the liquid of flourishing liquid portion be inhaled in the reactor or with the liquid discharge in the reactor.

As a further improvement of the above technical solution:

the transmission part comprises at least one piston, each reactor corresponds to one piston, two through holes communicated with the containing cavity in the reactor are formed in the reactor, the piston moves in one through hole or an extending hole of the through hole, the other through hole is communicated with the liquid containing part, and pressure is applied to the reactor when the piston moves, so that liquid in the liquid containing part is sucked into the reactor or the liquid in the reactor is discharged.

The hybridization part is also provided with a sealing pressing strip, the sealing pressing strip comprises a pressing strip body and a first transverse block positioned on the pressing strip body, at least one through hole is formed in the first transverse block, the reactor is connected with the sealing pressing strip, when the reactor is connected with the sealing pressing strip, the through hole in the first transverse block is communicated with the inside of the reactor, and one end of the piston is positioned in the through hole in the first transverse block.

Still be equipped with horizontal block two on the layering body, horizontal block one and the parallel interval arrangement of horizontal block two, horizontal block one is located the below of horizontal block two, be equipped with at least one through-hole on the horizontal block two, through-hole on the horizontal block one and the through-hole on the horizontal block two are the central line altogether, the one end of piston is located the through-hole on the horizontal block one, the other end is located between horizontal block one and the horizontal block two, transmission portion still includes at least one spring, the spring is located between horizontal block one and the horizontal block two, cup joint outside the piston, the top of horizontal block one is connected to the one end of spring, the one end that is located between horizontal block one and the horizontal block two of piston is connected to the other end.

The upper end of piston is equipped with spacing head, and the external diameter of spacing head is greater than the external diameter of piston, the external diameter of spacing head still is greater than the aperture of the through-hole on the horizontal block one and the aperture of the through-hole on the horizontal block two, spacing head is located between horizontal block one and horizontal block two, and the one end of spring is connected the top of horizontal block one is connected to spacing head, the other end.

The hybridization part is also provided with a frame body, the reactor is detachably arranged on the frame body, and the frame body is connected with the sealing pressing strip.

Liquid containing part comprises a test tube groove tray and a test tube groove, a plurality of grooves are arranged on the upper surface of the test tube groove tray at intervals, the grooves are used for directly containing liquid or placing test tubes used for containing liquid, and the test tube groove tray is detachably supported on the test tube groove.

The device is still including being located the liquid-transfering component of casing, and liquid-transfering component includes motor one, pulley transmission group one and linear guide one, and the test tube groove is installed on linear guide one, and motor one is through pulley transmission group transmission drive to linear guide one, and linear guide one drives test tube groove tray linear motion on test tube groove and the test tube groove.

The reaction assembly further comprises a needle tube, the needle tube is connected to the reactor, the needle tube is communicated with the inner cavity of the reactor, and liquid is sucked or discharged through the needle tube.

The drive division includes motor two, linear guide two, driving plate and at least one transfer line, and the driving plate is connected on linear guide two, and motor two pass through two transmission drives of linear guide to the driving plate, drive the driving plate and rise or descend, and the driving plate is connected to the upper end of transfer line, lower extreme overhang, and the external diameter of transfer line is less than the external diameter of the through-hole on the horizontal block two, and when the driving plate descends, the driving plate drives the transfer line and passes contact behind the through-hole on the horizontal block two and push down a plurality of pistons.

The utility model has the advantages that: reaction unit detachable installs in the hybridization appearance, the reaction of a plurality of reaction units can be accomplished once to the hybridization appearance, through imbibition flowing back device inhales liquid reaction unit or with the liquid discharge in the reaction unit, the reaction unit demolish after the reaction is accomplished can, make the transfer of liquid controllable, liquid can not pollute the hybridization appearance body, need not frequent and washing of degree of depth, improved the performance and the life-span of hybridization appearance.

Drawings

FIG. 1 is a schematic view of a liquid suction and drainage device in a hybridization apparatus according to an embodiment of the present invention.

Fig. 2 is a schematic view of fig. 1 with the thermal block removed.

Fig. 3 is a schematic structural view of a liquid containing part and a liquid-transfering component according to an embodiment of the present invention.

Fig. 4 is a schematic view of a frame structure according to an embodiment of the present invention.

Fig. 5 is a schematic structural diagram of a reaction module according to an embodiment of the present invention.

Fig. 6 is a schematic structural view of the sealing bead according to an embodiment of the present invention.

Detailed Description

The following describes in detail embodiments of the present invention with reference to the accompanying drawings. It is to be understood that the description of the embodiments herein is for purposes of illustration and explanation only and is not intended to limit the invention.

For ease of description, spatially relative terms such as "over 8230 \ 8230;,"' over 8230;, \8230; upper surface "," above ", etc. may be used herein to describe the spatial relationship of one device or feature to another device or feature as shown in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary terms "at 8230; \8230; above" may include both orientations "at 8230; \8230; above" and "at 8230; \8230; below". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

As shown in figures 1-6, the liquid sucking and draining device for hybridization instrument includes one casing, one hybridization part inside the casing, one liquid sucking and draining device, one transmission part, one driving part and one identifying and reading part.

The hybridization part is provided with a sealing depression bar 4, a heating component, a frame body 3, a lifting transmission component 6 and at least one group of reaction components 2.

The reaction assembly 2 comprises a needle tube 202 and a reactor 201, one end of the needle tube 202 is connected to the reactor 201, the other end of the needle tube 202 is suspended, an internal channel of the needle tube 202 is communicated with an internal cavity of the reactor 201, and a biochip is arranged in the internal cavity of the reactor 201. The front and rear panels of the reactor 201 are transparent and are disposed opposite to each other. The interior volume of the reactor 201 can be viewed or photographed through the front or rear panel. The upper end of the reactor 201 is provided with a through hole which is communicated with the cavity inside the reactor 201.

The support body 3 is used for the installation and supports reaction assembly 2, is equipped with a plurality of installation positions that are used for installing reaction assembly 2 on the support body 3, and is a plurality of installation position parallel interval arranges into one row. The upper end of the frame body 3 is also provided with a plurality of through holes which are respectively communicated with the installation positions. The reaction block 2 is detachably mounted on the mounting site. The frame body 3 is positioned above the liquid containing part 7. Reaction unit 2 installs back on support body 3, and reactor 201 card is gone into the installation position, reactor 201's inside appearance chamber, the through-hole of reactor 201 upper end, the through-hole of support body 3 upper end communicate in proper order, in order to improve the leakproofness of the passageway after the intercommunication, can set up the sealing washer in the upper end of reactor 201 and the junction of support body 3. Specifically, the reaction assembly 2 is clamped into the mounting position from the side surface of the frame body 3. The transparent front panel and the rear panel of reactor 201 are not sheltered from by

support body

3, and 3 positions of support body that correspond set up to the fretwork to supply follow-up shooting subassembly to shoot. The open end of the needle tube 202 faces downwards, the needle tube 202 extends out of the bottom of the rack body 3, and the lower ends of the needle tubes 202 of the reaction assemblies 2 on the plurality of mounting positions are respectively connected with the plurality of grooves on the test tube slot tray 701. By the operation of the subsequent transmission unit and the drive unit, the liquid in the cuvette tray 701 can be sucked into the reactor 201 through the needle 202.

The sealing pressing strip 4 is detachably connected with the upper end of the frame body 3, and the upper end face of the frame body 3 is attached to the lower end face of the sealing pressing strip 4. The sealing pressing strip 4 comprises a pressing strip body 401, and a first transverse block 402 and a second transverse block 403 which are located on the same surface of the pressing strip body 401. The first transverse block 402 and the second transverse block 403 are arranged in parallel at intervals, and the first transverse block 402 is positioned below the second transverse block 403. A plurality of parallel through holes in a row are arranged on the first transverse block 402 at intervals, a plurality of parallel through holes in a row are arranged on the second transverse block 403 at intervals, and the plurality of through holes on the first transverse block 402 and the plurality of through holes on the second transverse block 403 are on the same straight line. The number of through holes on the first transverse block 402, the number of through holes on the second transverse block 403 and the number of mounting positions on the frame body 3 are equal. Preferably, the aperture of the through hole on the first cross block 402 is equal to the aperture of the through hole on the second cross block 403. When the sealing pressing strip 4 is connected with the frame body 3, a plurality of through holes on the first cross block 402 are respectively communicated with a plurality of through holes at the upper end of the frame body 3. In order to improve the sealing performance of the communicated channel, a sealing ring can be arranged at the joint of the sealing pressing strip 4 and the frame body 3. The pressing strip body 401 is further provided with blind hole-shaped connecting holes, and the connecting holes and the first transverse block 402 are respectively located on two side faces of the pressing strip body 401.

The heating assembly is used to heat the reactor 201 of the reaction assembly 2 to provide temperature conditions for the reaction in the reactor 201. The heating assembly includes a thermal block 501 and wires. The hot block 501 is provided with an internal cavity, a plurality of holes communicated with the internal cavity are formed in the upper end opening and the lower end of the hot block 501 at intervals, the rack body 3 can be placed into the hot block 501 from the upper end of the hot block 501, and meanwhile, the needle tubes 202 of the reaction components 2 on the rack body 3 respectively penetrate through the holes in the lower end of the hot block 501 and then extend out of the hot block 501. That is, the frame body 3 is directly placed in the heat block 501 without connecting the two through a connecting member. The heating film is coated outside the heat block 501, the heating film is connected with an external power supply through a wire, heating of the heating film is achieved, and heat is transferred to liquid in the reactor 201 through the heat block 501. When the shelf 3 is placed on the heat block 501, the top of the shelf 3 is higher than the top of the heat block 501.

The lifting transmission assembly 6 comprises a magnet fixing frame, an electromagnet, a connecting rod and a return spring. The electro-magnet is installed on the magnet mount, and the connecting rod passes through return spring to be installed on the magnet mount. The electromagnet can produce an acting force on the connecting rod made of a metal material after being electrified, the connecting rod is driven to move, the connecting rod is inserted into a connecting hole in the pressing strip body 401 of the sealing pressing strip 4, the driving portion drives the magnet fixing frame to lift, the sealing pressing strip 4 and the frame body 3 are driven to lift through the connecting rod, and the frame body 3 can be separated from the hot block 501 after rising. When the electromagnet is powered off, the connecting rod returns under the elastic force of the return spring, and the connecting rod is withdrawn from the sealing pressing strip 4 and is separated from the contact with the sealing pressing strip 4.

The liquid suction and drainage device comprises a liquid containing part 7, a liquid transfer assembly 8, a transmission part and a driving part.

The liquid containing part 7 is used for containing liquid, and the liquid containing part 7 comprises a test tube groove tray 701 and a test tube groove 702. The upper surface of test tube groove tray 701 is parallel to the grooves of a plurality of long strips arranged at intervals, and the grooves are used for containing liquid or placing test tubes of the liquid. The cuvette tray 701 is detachably supported on the cuvette 702.

The pipetting assembly 8 is connected to and drives the liquid holding part 7 to extend out of or retract back into the housing. Specifically, the pipetting assembly 8 comprises a first motor 801, a first belt wheel transmission set 802, a first linear guide 803 and a test tube groove guide assembly. The test tube groove 702 is arranged on a first linear guide rail 803, a first motor 801 is driven to the first linear guide rail 803 through transmission of a first belt wheel transmission set 802, and the first linear guide rail 803 drives the test tube groove 702 and a test tube groove tray 701 on the test tube groove 702 to move linearly.

The first linear guide 803 comprises a base, a first screw rod and a first nut connecting block. The first screw rod is rotatably arranged on the base, the first nut connecting block is in threaded connection with the first screw rod, and when the first screw rod rotates, the first screw rod drives the first nut connecting block to move on the base along the length direction of the first screw rod.

The first belt wheel transmission set 802 comprises a belt wheel seat and two belt wheels, the two belt wheels are respectively connected with a motor shaft of the first motor 801 and one end of the first screw rod, and a transmission belt is tensioned on the two belt wheels.

The test tube slot guide assembly comprises an auxiliary guide rail 8041 and an auxiliary sliding block 8042, the auxiliary guide rail 8041 is fixedly installed in the shell, the sliding block 3042 is arranged on the guide rail 3041 in a sliding mode, and the sliding block 3042 is further connected with the auxiliary sliding block 8042. The auxiliary guide 8041 is arranged in parallel with the lead screw at a spacing. The test tube slot guide assembly can provide guidance for the test tube slot tray 701 and the test tube slot 702, so that the test tube slot tray 701 and the test tube slot 702 can move more stably.

The transmission portion includes a plurality of pistons 901 and a plurality of springs. One end of the piston 901 is provided with a stopper, the outer diameter of which is larger than the outer diameter of the piston 901. The outer diameter of the piston 901 is not larger than the aperture of the through hole on the first cross block 402 of the sealing pressing strip 4, and the outer diameter of the limiting head of the piston 901 is larger than the aperture of the through hole on the first cross block 402 and the aperture of the through hole on the second cross block 403. The piston 901 can extend into the through hole of the first cross block 402, and the circumferential surface of the piston 901 is attached to the wall of the through hole, so that the piston 901 can seal one end of the through hole of the first cross block 402. One end of the piston 901, which is far away from the limiting head, extends into the through hole in the first cross block 402, and the other end of the piston is located between the first cross block 402 and the second cross block 403, that is, the limiting head is located between the first cross block 402 and the second cross block 403, and the limiting head cannot extend into the through hole of the first cross block 402 or the through hole of the second cross block 403. The pistons 901 respectively extend into the through holes in the first cross block 402, and the pistons 901, the through holes in the first cross block 402 and the through holes in the second cross block 403 respectively share a center line. The spring is located between the first cross block 402 and the second cross block 403 and sleeved outside the piston 901, specifically, one end of the spring is connected with the top of the first cross block 402, and the other end of the spring is connected with the limiting head of the piston 901.

The driving part can be connected and drives the transmission part to act, so as to suck or discharge the liquid into or out of the reaction component 2. Meanwhile, the driving part can also drive the sealing pressing strip 4 and the frame body 3 to lift.

The driving part comprises a second motor 1001, a module fixing plate 1002, a second linear guide 1003, a transmission plate 1004 and a plurality of transmission rods 1005. The module fixing plate 1002 is fixedly installed in the shell, the second linear guide rail 1003 is installed on the module fixing plate 1002, the transmission plate 1004 is connected to the second linear guide rail 1003, and the second motor 1001 transmits drive to the transmission plate 1004 through the second linear guide rail 1003, so that the transmission plate 1004 ascends or descends. The magnet fixing frame of the lifting transmission assembly 6 is fixedly installed on the bottom surface of the transmission plate 1004, that is, the lifting transmission assembly 6 is driven by the driving part to ascend or descend.

The linear guide rail II 1003 comprises a guide rail seat, a screw rod II and a nut connecting block II, the screw rod II is rotatably installed on the guide rail seat, the screw rod II is perpendicular to the horizontal plane and is in threaded connection with the screw rod II, and when the screw rod II rotates, the screw rod II drives the nut connecting block II to move on the guide rail seat along the length direction of the screw rod II. The two sliding rails are arranged in parallel with the screw rod II, the two sliding rails are arranged on the module fixing plate 1002, and the two sliding rails are respectively arranged on two sides of the screw rod II. The two second sliding blocks are arranged on the two second sliding rails in a sliding mode respectively. One end of the transmission plate 1004 is connected with a second nut connecting block and a second sliding block at the same time, the second nut connecting block drives the transmission plate 1004 to move synchronously, and the second sliding block is used for guiding the movement of the transmission plate 1004, so that the movement of the transmission plate 1004 is more stable and stable.

A plurality of driving rods 1005 are arranged in parallel and at intervals in a row, one end of each driving rod 1005 is fixedly connected to the driving plate 1004, and the other end of each driving rod 1005 is suspended downwards. The number of drive rods 1005 is the same as the number of pistons 901. The outer diameter of the transmission rod 1005 is not larger than the aperture of the through hole on the second cross block 403. The transmission rods 1005 are respectively concentric with the through holes on the second cross block 403. When the driving plate 1004 descends, the driving rods 1005 are driven to descend and respectively pass through the through holes on the second cross block 403, then respectively contact one ends of the limiting heads of the pistons 901, when the driving rods 1005 continue to descend, the pistons 901 in contact with the driving rods are pressed downwards, and the springs are compressed. When the transmission rod 1005 ascends, the piston 901 is driven by the spring to ascend due to the action of the spring, and when the transmission rod 1005 enters the through hole on the second cross block 403, the piston 901 is separated from the contact with the transmission rod 1005 because the limiting head of the piston 901 cannot enter the through hole on the second cross block 403.

The recognition part is provided with a shooting component. The shooting component shoots the reacted reaction component 2 and transmits shooting information to a control system electrically connected with the shooting component.

All the motors, the shooting assembly, the circuit switch where the electromagnet is located, the circuit switch where the heating film of the heat block 501 is located and the like are electrically connected with an external control system.

The utility model discloses a working process does:

the working process of putting liquid into the liquid containing part 7 is as follows: the test tubes that will hold liquid are placed on the recess of test tube groove tray 701, can place a plurality of test tubes on every recess, and the array direction of a plurality of test tubes is parallel with test tube groove tray 701's removal direction. Firstly, the control system starts a first motor 801, so that the first motor 801 drives a first screw rod of a first linear guide 803 to rotate through a first belt wheel transmission set 802, the first screw rod drives a first nut connecting block to move along the length direction of the first screw rod, the first nut connecting block drives a test tube groove 702 and a test tube groove tray 701 connected with the first nut connecting block to move, and the test tube groove 702 and the test tube groove tray 701 extend out of the shell. Then, a test tube filled with a liquid is placed on the groove of the test tube well tray 701. Finally, after the joining is completed, the first motor 801 is started in a reverse direction, so that the test tube slot 702 and the test tube slot tray 701 are driven to move in a reverse direction to return to the shell. Preferably, the test tube slot 702 and the test tube slot tray 701 move step by step, so that each test tube moves to the position right below the needle tube 202 in turn for subsequent liquid suction.

The preferred, the recess complex liquid box of setting and test-tube groove tray 201, the liquid box includes box body and test-tube rack, and the test-tube rack is installed on the box body, be equipped with a plurality of test tubes on the test-tube rack, a plurality of test tubes are used for the different liquid of splendid attire respectively. The test tube rack is also provided with a through hole communicated with the box body, and the box body is also used for receiving waste liquid discharged by the needle tube 202. The direction of arrangement of the plurality of test tubes is parallel to the moving direction of the test tube well tray 201.

The working process of sucking liquid into the reactor 201 through the liquid suction and drainage device is as follows:

reaction conditions are provided by heating reactor 201 via thermal block 501 by drawing liquid into reactor 201 through a liquid take-off and draining liquid from reactor 201. The specific process is as follows:

step S1: the lifting transmission assembly 6 lifts the frame body 3, so that the needle tube 202 does not interfere with the movement of the test tube on the test tube slot tray 701.

Firstly, the circuit of the electromagnet is switched on, and at the moment, the connecting hole on the sealing pressing strip 4 is aligned with the connecting rod of the lifting transmission assembly 6 on the transmission plate 1004 at the initial position. After the electromagnet is electrified, acting force is generated between the electromagnet and the connecting rod, the connecting rod is driven to be inserted into a connecting hole in the pressing strip body 4011 of the sealing pressing strip 4, and the electromagnet is kept electrified.

Then, the second motor is started, the power of the second motor is transmitted to the transmission plate 1004 through the second linear guide rail, the transmission plate 1004 is driven to ascend by the second linear guide rail, the connection rod on the transmission plate 1004 is driven to ascend, and the connection rod drives the sealing pressing strip 4, the frame body 3 and the reactor 201 to ascend synchronously.

Step S2: the cuvette tray 701 moves, and when a cuvette containing liquid is moved to a position directly below the needle 202, the movement of the cuvette tray 701 is stopped.

And step S3: the lifting transmission assembly 6 drives the frame body 3 to descend, so that the needle tube 202 is inserted into the test tube.

In this step, the second motor is reversely started, the transmission plate 1004 and the connecting rod are driven to descend, and the connecting rod drives the sealing pressing strip 4, the frame body 3 and the reactor 201 to descend synchronously, so that the frame body 3 enters and is supported on the heat block 501 again.

And step S4: the aspirating and draining assembly is actuated and the needle 202 draws the fluid in the test tube into the interior volume of the reactor 201.

In this step, firstly, the second motor is started through the control system, so that the power of the second motor is transmitted to the transmission plate 1004 through the second linear guide rail, at this time, the circuit connected with the electromagnet is in a disconnected state, and the connecting rod of the lifting transmission assembly 6 is not connected with the sealing pressing strip 4. The driving plate 1004 is driven to descend by the second linear guide rail, the driving rods 1005 on the driving plate 1004 respectively extend into and penetrate through the through holes on the second cross block 403, after the driving rods 1005 respectively contact the pistons 901, the pistons 901 are pressed, the pistons 901 respectively descend along the through holes on the first cross block 402, and at the moment, springs outside the pistons 901 are compressed. As the piston 901 descends, the gas in the through holes below the piston 901 on the first cross block 402 and the cavity of the reactor 201 is discharged outwards.

Then, the second motor is started reversely, so that the power of the second motor is transmitted to the transmission plate 1004 through the second linear guide rail, the transmission plate 1004 is driven by the second linear guide rail to ascend, the piston 901 ascends under the action of the elastic force of the spring until the limiting head of the piston 901 contacts the bottom of the second cross block 403, and cannot ascend any more, and the spring is still in a compressed state at the moment, so that the current state of the piston 901 can be stably maintained. During the process of the piston 901 rising, the liquid in the test tube will be sucked into the cavity of the reactor 201 through the needle tube 202, similar to the principle of a syringe. The driving plate 1004 continues to rise to the initial position.

Step S5: the lifting transmission assembly 6 lifts the rack body 3, the test tube slot tray 701 moves, and when the rack body is moved to the position below the needle tube 202, the test tube slot tray 701 stops moving.

Step S6: the sipping and draining assembly is actuated and the liquid in the chamber of the reactor 201 is drained. The operation of the liquid suction and discharge assembly in this step is the same as that in step S4.

Step S7: the steps S1 to S6 are repeated to suck the liquid in another test tube into the needle tube 202.

When the reaction vessel 201 finishes sucking the liquid in each test tube in turn, the reaction is completed. After each aspiration of liquid, a procedure is performed in the reactor 201, such as hybridization, washing, coloring, etc.

During the reaction, the thermal block 501 transfers heat to the reactor 201. Specifically, a circuit where a heating film outside the thermal block 501 is located is conducted, the heating film is electrified and heated, and heat is transferred to the reactor 201 in the thermal block 501 through the thermal block 501 to provide reaction conditions for the liquid in the reactor 201.

Finally, it must be said here that: the above embodiments are only used for further detailed description of the technical solutions of the present invention, and should not be understood as limiting the scope of the present invention, and the modifications and adjustments made by those skilled in the art according to the above-mentioned contents of the present invention are all included in the scope of the present invention.

Claims

1. The utility model provides a imbibition flowing back device of hybridization appearance, characterized in that, hybridization appearance includes the casing, is equipped with hybridization portion and imbibition flowing back device in the casing, and hybridization portion is equipped with at least a set of reaction unit (2), and reaction unit (2) include one reactor (201), are equipped with the biochip in reactor (201), imbibition flowing back device is including flourishing liquid portion (7), transmission and drive division, and flourishing liquid portion (7) have liquid, and the one end and flourishing liquid portion (7) intercommunication, the transmission is connected to the other end of reactor (201), and the transmission is connected and drive transmission, and transmission exerts pressure in to reactor (201), makes the liquid of flourishing liquid portion (7) be inhaled in reactor (201) or with the liquid discharge in the reactor (201).

2. The liquid imbibing and draining device of claim 1, wherein: the transmission part comprises at least one piston (901), each reactor (201) corresponds to one piston (901), two through holes communicated with the containing cavity in the reactor (201) are formed in the reactor (201), the pistons (901) move in one through hole or an extension hole of the through hole, the other through hole is communicated with the liquid containing part (7), and when the pistons (901) move, pressure is applied to the inside of the reactor (201) to enable liquid in the liquid containing part (7) to be sucked into the reactor (201) or discharge the liquid in the reactor (201).

3. The liquid imbibition drain of claim 2 wherein: the cross section is further provided with a sealing pressing strip (4), the sealing pressing strip (4) comprises a pressing strip body (401) and a first transverse block (402) located on the pressing strip body (401), at least one through hole is formed in the first transverse block (402), the sealing pressing strip (4) is connected with the reactor (201), when the reactor (201) is connected with the sealing pressing strip (4), the through hole in the first transverse block (402) is communicated with the inside of the reactor (201), and one end of a piston (901) is located in the through hole in the first transverse block (402).

4. The wicking drain of claim 3, wherein: the pressing strip body (401) is further provided with a transverse block II (403), the transverse block I (402) and the transverse block II (403) are arranged in parallel at intervals, the transverse block I (402) is located below the transverse block II (403), the transverse block II (403) is provided with at least one through hole, the through hole in the transverse block I (402) and the through hole in the transverse block II (403) share a center line, one end of a piston (901) is located in the through hole in the transverse block I (402), the other end of the piston is located between the transverse block I (402) and the transverse block II (403), the transmission portion further comprises at least one spring, the spring is located between the transverse block I (402) and the transverse block II (901) and sleeved outside the piston (901), one end of the spring is connected with the top of the transverse block I (402), and the other end of the spring is connected with one end, located between the transverse block I (402) and the transverse block II (403), of the piston (901).

5. The liquid aspirating and draining apparatus of claim 4, wherein: the upper end of the piston (901) is provided with a limiting head, the outer diameter of the limiting head is larger than that of the piston (901), the outer diameter of the limiting head is also larger than the aperture of the through hole in the first transverse block (402) and the aperture of the through hole in the second transverse block (403), the limiting head is positioned between the first transverse block (402) and the second transverse block (403), one end of the spring is connected with the limiting head, and the other end of the spring is connected with the top of the first transverse block (402).

6. A liquid suction and discharge device according to any one of claims 3 to 5, wherein: the hybridization part is also provided with a frame body (3), the reactor (201) is detachably arranged on the frame body (3), and the frame body (3) is connected with the sealing pressing strip (4).

7. The liquid imbibing and draining device of claim 1 wherein: liquid containing part (7) comprises a test tube groove tray (701) and a test tube groove (702), a plurality of grooves are arranged on the upper surface of the test tube groove tray (701) at intervals, the grooves are used for directly containing liquid or placing test tubes used for containing liquid, and the test tube groove tray (701) is detachably supported on the test tube groove (702).

8. The liquid wicking and drainage device of claim 7 wherein: the device is still including lieing in liquid-transfering component (8) in the casing, and liquid-transfering component (8) include motor one (801), pulley transmission group one (802) and linear guide one (803), and test tube groove (702) are installed on linear guide one (803), and motor one (801) pass through pulley transmission group one (802) transmission drive to linear guide one (803), and linear guide one (803) drive test tube groove tray (701) linear motion on test tube groove (702) and test tube groove (702).

9. The liquid imbibition drain of claim 2 wherein: the reaction assembly (2) further comprises a needle tube (202), the needle tube (202) is connected to the reactor (201), the needle tube (202) is communicated with the inner cavity of the reactor (201), and liquid is sucked or discharged through the needle tube (202).

10. The wicking drain of claim 4, wherein: the driving part comprises a second motor (1001), a second linear guide rail (1003), a transmission plate (1004) and at least one transmission rod (1005), the transmission plate (1004) is connected to the second linear guide rail (1003), the second motor (1001) is driven to the transmission plate (1004) through the second linear guide rail (1003), the transmission plate (1004) is driven to ascend or descend, the transmission plate (1004) is connected to the upper end of the transmission rod (1005), the lower end of the transmission rod (1005) is suspended, the outer diameter of the transmission rod (1005) is smaller than that of a through hole in the second transverse block (403), and when the transmission plate (1004) descends, the transmission plate (1004) drives the transmission rod (1005) to penetrate through holes in the second transverse block (403) and then to contact with and press down a plurality of pistons (901).