CN108456640A - The PCR modules of diagnosis all-in-one machine are expanded for full-automatic nucleic acid extraction - Google Patents

Abstract

The invention discloses a kind of PCR modules for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, have cover board, several reagent wells are provided on the cover board；It is provided with hot seat corresponding with the reagent wells below cover board, heating plate and amplification radiator are installed in the bottom of hot seat；Heating plate is located between hot seat and the amplification radiator, expands and is connected with nylon cylinder between radiator and cover board；Hot seated connection enters to have optical fiber.The PCR modules of above structure, can accomplish being capable of sample i.e. checking functions, detection mode is flexible, hot seated connection enters to have optical fiber, each hot seat has corresponding reagent wells, can individually be heated to the nucleic acid in reagent wells, and it is independent of each other between adjacent reagent wells, the temperature of single hot seat every bit is identical, and temperature is uniformly distributed, and detection result is more preferably；Fiber optic conduction technology is used, it is compact-sized among original very large PCR equipment is contracted to slight PCR modules.

Description

The PCR modules of diagnosis all-in-one machine are expanded for full-automatic nucleic acid extraction

Technical field

The invention belongs to technical field of medical equipment, more particularly, to one kind for full-automatic nucleic acid extraction amplification diagnosis one The PCR modules of body machine.

Background technology

When carrying out molecule diagnosis, most of molecule diagnosis are all semi-automated instruments currently on the market, need to carry out artificial It is put into the nucleic acid step extracted, quantitative analysis is being carried out by instrument so that increase the generated test of manual intervention Error, test speed is slow, is manually placed into the nucleic acid extracted and be easy to cause biological pollution, and is examined in automatically extraction amplifier molecule In disconnected equipment, the temperature control of PCR gene amplification instrument is also difficult point, and PCR gene amplification instrument is a for providing simulation gene Temperature device when amplification needed for each stage, and increase the functions such as low-temperature storage and fluoroscopic examination, it is suitable for human gene Group engineering science, the research fields such as medical jurisprudence, oncology, tissue and Population Biology, paleontology, zoology, botany and disease The polymerase chain reaction fluorogenic quantitative detection in the clinical diagnosises such as poison, tumour, hereditary disease field.

In Chinese patent literature CN103160426B, a kind of PCR gene amplification instrument module mechanism, including module are disclosed Ontology is additionally provided with and multiple biographies for the close-connected module heat conduction of module body equal plate and being arranged on the equal plate of module heat conduction Sensor fixed column is provided with several test tube hole slots, bottom and the test tube hole channel opening side of test tube hole slot in the module body It is equipped with several heating columns for corresponding to test tube hole slot, end face and the equal plate of module heat conduction of the heating column to opposite side Plate face pressing together；The heating column is cylindrical structure, the pyrotoxin being arranged distant from below the equal plate of module heat conduction Heating column diameter be more than pyrotoxin center at heat conduction column diameter；The test tube hole slot is distributed on module in matrix structure On ontology.

But the PCR gene amplification instrument module mechanism in above-mentioned technical proposal is less desirable in the specific use process, temperature Degree controls poor, detection mode underaction.

Invention content

The technical problem to be solved in the present invention is to provide a kind of temperature to control, compact-sized, and detection mode is flexibly used The PCR modules of diagnosis all-in-one machine are expanded in full-automatic nucleic acid extraction.

In order to solve the above technical problems, the technical solution adopted by the present invention is, this is used for full-automatic nucleic acid extraction amplification and examines The PCR modules of disconnected all-in-one machine have cover board, several reagent wells are provided on the cover board；Be provided with below the cover board with The corresponding hot seat of reagent wells is equipped with heating plate and amplification radiator in the bottom of the hot seat；The heating plate Between the hot seat and the amplification radiator, connected with nylon cylinder between amplification radiator and the cover board；It is described Hot seated connection enters to have optical fiber.

The PCR modules of above structure, can accomplish can sample i.e. checking functions, detection mode is flexible, and hot seated connection enters to have light Fibre can detect four kinds of indexs of sample simultaneously using four color fluorescent techniques；Fiber optic conduction technology is used, it will be original very large PCR equipment is contracted among slight PCR modules, compact-sized；Also accomplish that each sample is individually examined simultaneously, does not interfere with each other； Heating plate and amplification radiator be installed in the bottom of hot seat, heating plate be located at the hot seat and the amplification radiator it Between, it is more prone to control temperature；Each hot seat has corresponding reagent wells, can individually be carried out to the nucleic acid in reagent wells Heating, the hot each temperature of seat is not necessarily identical, can individually control, and be independent of each other between adjacent reagent wells, single heat The temperature of seat every bit is identical, and temperature is uniformly distributed, and detection result is more preferably；Solves integrated function individually to each reagent The problem for carrying out heating amplification, improves the test speed of all-in-one machine.

Further improve is, conducting wire is provided in the heating plate；The hot seat is made of hollow circuit cylinder hole, in institute The centre position for stating hot seat laterally projecting has insertion hole, the optical fiber to be accessed in the hot seat by the insertion hole.

By wire-heated, can be contacted multiple heating plates with conducting wire, and mode of heating is more convenient；Hot seat is by hollow circuit cylinder Hole is constituted, and EP pipes can be put by reagent wells in corresponding hot seat.

Further improve is, 16 reagent wells are provided on the cover board, corresponding, and the hot seat also has 16； It is provided with step in the reagent wells.

Multiple reagent wells, amount detection is more, and step is provided in reagent wells, and detection is facilitated to operate.

Further improve is that the amplification radiator includes the upper surface for installing the heating plate；The upper end Two opposite sides of face are extended with the first cooling fin and the second cooling fin vertically downward；First cooling fin and the second heat dissipation It is additionally provided with several inner fins between piece, there is heat dissipation gap between two neighboring inner fin.

Pass through the frame mode of cooling fin so that amplification radiator heat-dissipation is more convenient, and the temperature for controlling hot seat is more held It easily realizes, and simple in structure, it is at low cost；Amplification radiator is connect by upper surface with heating plate, and heat dissipation is more direct.

Further improve is that the reagent wells are evenly distributed on the cover board；It is provided on the cover board and institute State nylon cylinder mounting hole in mating connection.

It is connected using nylon cylinder, plays heat-blocking action；Reagent wells are uniformly distributed independently of each other, corresponding with reagent wells Hot seat is also uniformly distributed.

Further improve is that the bottom of the hot seat also has mounting plate, and the mounting plate and the heating plate are straight It connects in succession.The effect of mounting plate is set so that being uniformly heated for hot seat, temperature control are more stable.

Further improve is that the heating plate is fixed between hot seat and amplification radiator and has thermal insulation board cladding, Prevent heat loss.

Further improve is that the amplification radiator is mounted on each hot seat lower part with the nylon cylinder, this Sample does not interfere with the temperature of hot seat.

The invention solves another technical problem be to provide it is a kind of need not carry out being manually placed into the nucleic acid extracted, Measuring accuracy is high, and the full-automatic nucleic acid extraction amplification diagnosis all-in-one machine that the testing time is short.

In order to solve the above technical problems, the technical solution adopted by the present invention is, the full-automatic nucleic acid extraction amplification diagnosis one Body machine includes the holder of pedestal and setting on the base, further includes having：

Nucleic acid extraction module is used for purifying nucleic acid, and the nucleic acid extraction module is provided with reaction platform, and reaction cup is placed On the reaction platform；

Sample access mechanism, for preserving sample, the sample access mechanism is located at the side of the nucleic acid extraction module And be placed on the pedestal, the sample access mechanism is provided with sample rack；

There is mechanical arm mechanism Y-axis horizontal box, the vertical babinet of Z axis to do Y-axis horizontal movement along the Y-axis horizontal box； Sampling head moves up and down on the vertical babinet of the Z axis；The Y-axis horizontal box is connected with the holder；

Hardware installation module, for installing hardware, the hardware installation module setting the Y-axis horizontal box with it is described Holder is connected the lower section at position；

PCR modules, for detecting sample, the PCR modules setting is in hardware installation module side and positioned at described Below holder；

Biohazard Waste containing box includes to be arranged in the sample access mechanism and institute for storing Biohazard Waste The containing box between PCR modules is stated, and is installed on the pedestal；

Reagent storing mechanism is arranged for storing reagent in the top of the Biohazard Waste containing box；

Reaction cup sample introduction mechanism is arranged in the front end of the nucleic acid extraction module, and on the pedestal.

Using above-mentioned technical proposal, all parts are incorporated on pedestal and form complete equipment, structure-integrated, flowing water Change operation, the testing time is short, and using effect is good, and reaction cup is pushed by reaction cup sample introduction mechanism in nucleic acid extraction module, Using mechanical arm mechanism from sampled in sample access mechanism be originally added to reaction cup packaging consumptive material in reaction cup in, reaction terminates Afterwards, nucleic acid is extracted by mechanical arm mechanism into the EP pipes of reagent storing mechanism, and reaction cup waste is focused on to medical waste In object containing box；EP pipes equipped with reagent are extracted to PCR modules from reagent storing mechanism expand again by mechanical arm mechanism Detection；Integral module is designed using enclosed modulesization, has prevented equipment pollution problem caused by due to nucleic acid extraction；Using machine Tool arm mechanism transfer nucleic acid, has prevented the influence to influence each other for detection data by intermodule；Hardware installation module will own Circuit board is integrated within hardware module, has prevented external electromagnetic interference problem, to prevent to make because of electromagnetic interference At data fluctuations, measuring accuracy is high.

Further improve be, the nucleic acid extraction module has a movable plate component, the movable plate component by upper moving plate with And the lower moving plate being equipped with the upper moving plate is constituted；Reaction platform and dustbin, institute are provided with below the movable plate component State the front end that reaction platform is located at the movable plate component；Driving lever component is provided with below the reaction platform, in driving lever component With the driving lever pushed reaction cup waste in the dustbin；The driving lever component also has driving lever sliding rail, the driving lever Driving lever sliding block is installed, the driving lever is arranged on driving lever sliding block on sliding rail.

After reaction, reaction cup waste is pushed in the dustbin, then Biohazard Waste is gone to by dustbin week Containing box, convenient and efficient, process is pollution-free；Driving lever sliding block allows driving lever to be moved in X axis, promotes each reaction cup, After reaction, reaction cup waste is pushed in the dustbin.

Further improve is that the movable plate component further includes having upper moving plate lead screw motor and lower moving plate lead screw motor, The bar magnet installed on the upper moving plate drives magnetosheath thereon to move up and down under the driving of the upper moving plate lead screw motor, institute It states the decylization installed on lower moving plate and sloughs magnetosheath on the bar magnet under the driving of the lower moving plate lead screw motor.

Further improve is that the reaction platform includes platform framework, and the platform framework has several reactions Framework has reaction compartment, the rear and front end of the platform framework to be both provided with cover board between two neighboring reaction framework.

Reaction compartment makes the reaction cup being located in reaction frame body have certain distance, avoids and has prevented due to nucleic acid extraction Caused by pollution problem；The rear and front end of platform framework is both provided with cover board and plays work that is anti-pollution and reinforcing reaction platform With.

Further improve be, the sample access mechanism by positioned at top half sample pane and be located at lower half portion Sample radiator constitute；It is provided with sample cover board in the sample pane, is surrounded by by the sample pane and the sample cover board Several mutually independent sample rack accommodating cavities；The sample rack is placed in the sample rack accommodating cavity, in the sample rack The bottom of accommodating cavity is provided with sliding rail；The back side of the sample pane is provided with the sample area control circuit board with detector；It is described If the cooling piece that sample radiator has dry plate spaced.

Sample access mechanism is divide into upper part and lower part, compact-sized, lower half portion is arranged to heat spreader structures, both fully Its function is played, and does not influence the work of other component such as mechanical arm mechanism；Sample rack is promoted to hold sample rack by sliding rail Chamber is set, it is convenient and efficient.

Further improve is have several test tube suit holes on the sample rack, be arranged on the sample cover board There is access port identical with test tube suit hole number on the sample rack；The sample rack bottom has to be matched with the sliding rail Sliding-rail groove；Mechanical arm mechanism captures the sample on sample rack by access port.

Further improve is that the reagent storing mechanism and the structure of the sample access mechanism are identical.

Further improve is that the external side of Z axis vertical box of the mechanical arm mechanism is provided with sampling lead screw motor, with The sampling lead screw linkage being arranged in the Z axis vertical box body is connected, and sampling head, the sampling are provided on the sampling lead screw Head vertical babinet of Z axis described in the driving lower edge of the sampling lead screw motor moves up and down；The outside of the Y-axis horizontal box It is provided with mobile lead screw motor, is connected with the mobile lead screw linkage being arranged in the Y-axis horizontal box, the Z axis vertical box Slide block set on body is on the mobile lead screw, sliding block Y-axis filter box described in the driving lower edge of mobile lead screw motor Body does Y-axis horizontal movement.

It further improves and is, three face hollow out of the Y-axis horizontal box；And the sampling head is arranged by clamping element On the sampling lead screw, the clamping element is sleeved on the sampling lead screw；By using clamping element, sampling head can be carried out Adjustment is replaced, ensures that sampling is accurate.

Further improve is that the hardware installation module is set with by electro-magnetic shielding cover and heat sink, and described If being provided with dry plate hardware installation plate on heat sink, there is nylon column on the hardware installation plate；There is heat dissipation on the heat sink Hole.

Electro-magnetic shielding cover can fall most electromagnetic shielding, and all circuit boards are integrated within hardware module, are shut out External electromagnetic interference problem absolutely, to prevented because electromagnetic interference and caused by data fluctuations.

Further improve is that the Biohazard Waste containing box further includes having reaction cup waste conveyer and storage The end lower section of chamber lid, the reaction cup waste conveyer has storage mouth, the storage mouth to be arranged in the Drawer-and-shell type box It covers；Cover in the side of the reaction cup waste conveyer and positioned at the Drawer-and-shell type box that be provided with substitute head waste de- Handler；The Biohazard Waste containing box further includes having containing box guide rail, is arranged on the base, the containing box box cover It is equipped by containing box guide-track groove arranged at the bottom and the containing box guide rail；It is additionally provided on the outside of the containing box box cover Handle.

Further improve is that there is the reaction cup sample introduction mechanism reaction cup box, the reaction cup box to pass through sample introduction Holder is installed on the base；Reaction cup is assemblied in reaction cup packaging consumptive material, is pushed into the reaction cup box；The reaction One end of cup sample introduction mechanism is additionally provided with sample introduction motor, and the sample introduction motor has synchronous pulley, in the reaction cup into model machine The other end of structure is additionally provided with and the matched idle pulley of the synchronous pulley；It is wrapped on the synchronous pulley and the idle pulley Synchronous belt, that section being located above be placed in the reaction cup box, that underlying section is placed in the reaction cup box Outside；Reaction cup push plate is additionally provided on the synchronous belt.

Description of the drawings

It is further described below in conjunction with the accompanying drawings with embodiments of the present invention：

Fig. 1 is full-automatic nucleic acid extraction amplification diagnosis all-in-one machine structure schematic diagram of the invention；

Fig. 2 is the nucleic acid extraction modular structure schematic diagram in Fig. 1；

Fig. 3 is the sample access mechanism structural schematic diagram in Fig. 1；

Fig. 4 is the mechanical arm mechanism structural schematic diagram in Fig. 1；

Fig. 5 is the hardware installation modular structure schematic diagram in Fig. 1；

Fig. 6 is the PCR modular structure schematic diagrams in Fig. 1；

Fig. 7 is the Biohazard Waste housing box structure schematic diagram in Fig. 1；

Fig. 8 is the reagent storing mechanism structural schematic diagram in Fig. 1；

Fig. 9 is the reaction cup sample introduction mechanism structural schematic diagram in Fig. 1；

Figure 10 is PCR decomposition module structures schematic diagram in Fig. 6；

Figure 11 is the part-structure decomposition diagram in Figure 10；

Wherein：1- nucleic acid extraction modules；101- upper moving plates；102- lower moving plates；103- upper moving plate lead screw motors；104- rubbish Case；105- bar magnets；106- decylizations；107- driving levers；108- reacts platform；109- movable plate components；110- driving lever components；111- driving levers Sliding rail；112- driving lever sliding blocks；113- lower moving plate lead screw motors；114- magnetosheaths；115- platform frameworks；116- reacts framework；117- Reaction compartment；118- cover boards；2- sample access mechanisms；The sample areas 201- control circuit board；202- sample cover boards；203- samples Frame；204- sample panes；205- sample radiators；206- sliding rails；207- sample rack accommodating cavities；208- cooling pieces；209- test tube sleeves Fill hole；210- access ports；211- sliding-rail grooves；3- mechanical arm mechanisms；301-Y axis horizontal box；302- sliding blocks；303- samples lead screw Motor；304- moves lead screw；305- moves lead screw motor；The vertical babinet of 306-Z axis；307- sampling heads；308- samples lead screw 309- clamping elements；4- hardware installation modules；401- electro-magnetic shielding covers；402- hardware installation plates；403- nylon columns；404- radiates Plate；405- heat emission holes；5-PCR modules；501- cover boards；502- heat seats；503- heating plates；504- optical fiber；505- expands radiator； 506- reagent wells；507- nylon cylinders；508- conducting wires；509- is inserted into hole；510- steps；The upper surfaces 511-；512- first radiates Piece；The second cooling fins of 513-；514- inner fins；515- heat dissipations gap；516- mounting holes；517- mounting plates；6- medical treatment is useless Gurry containing box；601- reaction cup waste conveyers；602- substitute head waste disconnecting means；603- containing box box covers； 604- handles；605- containing box guide rails；606- stores mouth；607- containing box guide-track grooves；7- reagent storing mechanisms；8- reaction cups into Model machine structure；801- reaction cups；802- reaction cup boxes；803- reaction cups pack consumptive material；804- idle pulleys；805- reaction cup push plates； 806- synchronous belts；807- synchronous pulleys；808- sample introduction motors；809- sample introduction holders；9- pedestals；10- holders.

Specific implementation mode

Include pedestal 9 and setting as shown in Figure 1, being the full-automatic nucleic acid extraction amplification diagnosis all-in-one machine of the present invention Holder 10 on the pedestal 9, further includes having：

Nucleic acid extraction module 1 is used for purifying nucleic acid, and the nucleic acid extraction module 1 is provided with reaction platform 108, reaction Cup 801 is placed on the reaction platform 108；

Sample access mechanism 2, for preserving sample, the sample access mechanism 2 is located at the side of the nucleic acid extraction module 1 It face and is placed on the pedestal 9, the sample access mechanism 2 is provided with sample rack 203；

There is mechanical arm mechanism 3 Y-axis horizontal box 301, the vertical babinet of Z axis 306 to be Y along the Y-axis horizontal box 301 Axis horizontal movement；Sampling head moves up and down on the vertical babinet of the Z axis 306；The Y-axis horizontal box 301 and the branch Frame 10 is connected；

Hardware installation module 4, for installing hardware device and/or other affiliated facilities, the hardware installation module 4 is set Set in the Y-axis horizontal box 301 lower section at the position that is connected with the holder 10；

PCR modules 5, for detecting sample, the setting of PCR modules 5 is in 4 side of hardware installation module and is located at institute State 10 lower section of holder；

Biohazard Waste containing box 6 includes to be arranged in 2 He of sample access mechanism for storing Biohazard Waste Containing box box cover 603 between the PCR modules 5, and be installed on the pedestal 9；

Reagent storing mechanism 7 is arranged for storing reagent in the top of the Biohazard Waste containing box 6；

Reaction cup sample introduction mechanism 8 is arranged in the front end of the nucleic acid extraction module 1, and on the pedestal 9.

As shown in Fig. 2, nucleic acid extraction module 1 has a movable plate component 109, the movable plate component 109 by upper moving plate 101 and It is constituted with the lower moving plate 102 that the upper moving plate 101 is equipped with；The lower section of the movable plate component 109 is provided with reaction 108 He of platform Dustbin 104, the reaction platform 108 are located at the front end of the movable plate component 109；The lower section setting of the reaction platform 108 There is driving lever component 110, there is the driving lever 107 pushed reaction cup waste in the dustbin 104 in driving lever component 110；Institute Stating driving lever component 110 also has driving lever sliding rail 111, and driving lever sliding block 112, the driving lever 107 are equipped on the driving lever sliding rail 111 It is arranged on driving lever sliding block 112.

Movable plate component 109 further includes having upper moving plate lead screw motor 103 and lower moving plate lead screw motor 113, the upper moving plate 101 The bar magnet 105 of upper installation drives magnetosheath 114 thereon to move up and down under the driving of the upper moving plate lead screw motor 103, institute It states the decylization 106 installed on lower moving plate 102 and sloughs magnetic on the bar magnet 105 under the driving of the movable plate lead screw motor 113 Set 114；Can be with such, movable plate component is by several mutually independent upper moving plates and several mutually independent lower moving plates It constitutes, a upper moving plate corresponds with a lower moving plate, compounding practice, ensure that the independence of nucleic acid extraction operation and efficient Property；It includes platform framework 115 to react platform 108, and the platform framework 115 has several to react framework 116, two neighboring anti- Answering between framework 116 has reaction compartment 117, the rear and front end of the platform framework 115 to be both provided with cover board 118.

As shown in figure 3, sample access mechanism 2 by positioned at top half sample pane 204 and positioned at the sample of lower half portion Radiator 205 is constituted；Sample cover board 202 is provided in the sample pane 204, by the sample pane 204 and the sample cover board 202 are surrounded by several mutually independent sample rack accommodating cavities 207；The sample rack 203 is placed on the sample rack accommodating cavity In 207, the bottom of the sample rack accommodating cavity 207 is provided with sliding rail 206；The back side of the sample pane 204 is provided with band inspection Survey the sample area control circuit board 201 of device；If the cooling piece 208 that the sample radiator 205 has dry plate spaced；Sample There are several test tube suit holes 209 on frame 203, be provided with and test tube sleeve on the sample rack 203 on the sample cover board 202 Fill the identical access port of 209 quantity of hole 210；203 bottom of the sample rack has and 206 matched sliding-rail groove of the sliding rail 211。

In embodiments of the present invention, reagent storing mechanism 7 and the structure of the sample access mechanism 2 are identical, such as Fig. 8 It is shown；The mechanism has reagent rack to be put into EP pipes to promote along reagent frame along sliding rail, and reagent area control circuit board carries detector, can be with Whether induction has a reagent entrance, and bottom is by there is cooling piece that reagent can be made to be in frozen state always.

As shown in figure 4, vertical 306 outside of babinet of the Z axis of the mechanical arm mechanism 3 is provided with sampling lead screw motor 303, with The linkage of sampling lead screw 308 being arranged in the vertical babinet of the Z axis 306 is connected, and sampling head is provided on the sampling lead screw 308 307, the vertical babinet of Z axis 306 described in the driving lower edge of the sampling lead screw motor 303 of the sampling head 307 moves up and down； The outside of the Y-axis horizontal box 301 is provided with mobile lead screw motor 305, and is arranged in the Y-axis horizontal box 301 The mobile linkage of lead screw 304 is connected, and the sliding block 302 on the vertical babinet of the Z axis 306 is sleeved on the mobile lead screw 304, described The Y-axis horizontal box 301 described in the driving lower edge of mobile lead screw motor 305 of sliding block 302 does Y-axis horizontal movement.

In the present embodiment, 301 3 face hollow out of Y-axis horizontal box；And the sampling head 307 is arranged by clamping element 309 On the sampling lead screw 308, the clamping element 309 is sleeved on the sampling lead screw 308.

As shown in figure 5, hardware installation module 4 is set with by electro-magnetic shielding cover 401 and heat sink 404, and the heat dissipation If being provided with dry plate hardware installation plate 402 on plate 404, there is nylon column 403 on the hardware installation plate 402；The heat sink There is heat emission hole 405 on 404.

As shown in fig. 6, PCR modules 5 have cover board 501, several reagent wells 506 are provided on the cover board 501；Described The lower section of cover board 501 is provided with hot seat 502 corresponding with the reagent wells 506, is equipped with and adds in the bottom of the hot seat 502 Backing 503 and amplification radiator 505；The heating plate 503 between the hot seat 502 and the amplification radiator 505, It is connected with nylon cylinder 507 between the amplification radiator 505 and cover board 501；The access of hot seat 502 has optical fiber 504.

As shown in Figure 10, it is 5 decomposition texture schematic diagram of PCR modules, it can be seen that the structure in terms of more details, heating plate Two conducting wires 508 are provided in 503；The hot seat 502 is made of hollow circuit cylinder hole, horizontal in the centre position of the hot seat 502 It is inserted into hole 509 to protruding, the optical fiber 504 is accessed to by the insertion hole 509 in the hot seat 502, in the present embodiment, 16 reagent wells 506 are provided on the cover board 501, corresponding, the hot seat 502 also has 16；It is set in the reagent wells 506 It is equipped with step 510, the amplification radiator 505 includes the upper surface 511 for installing the heating plate 503；The upper surface 511 Two opposite sides are extended with the first cooling fin 512 and the second cooling fin 513 vertically downward；First cooling fin, 512 He It is additionally provided with several inner fins 514 between second cooling fin 513, has between two neighboring inner fin 514 and dissipates Temperature gap 515, the reagent wells 506 are evenly distributed on the cover board 501；It is provided on the cover board 501 and the nylon The mounting hole 516 in mating connection of cylinder 507, the bottom of the hot seat 502 also have a mounting plate 517, the mounting plate 517 with The heating plate 503 is directly connected to；The heating plate 503 is fixed between hot seat 502 and amplification radiator 505 and has thermal insulation board (not shown) coats；It expands radiator 505 and is mounted on each 502 lower part of hot seat with the nylon cylinder 507；It is visible in Figure 11 The EP pipes being located above are inserted into reagent wells 506.

As shown in Figure 7, the Biohazard Waste containing box 6 further includes having reaction cup waste conveyer 601 and storage The end lower section of chamber lid 603, the reaction cup waste conveyer 601 has storage mouth 606, the setting of storage mouth 606 to exist On the containing box box cover 603；In the side of the reaction cup waste conveyer 601 and it is located at the containing box box cover 603 On be provided with substitute head waste disconnecting means 602；The Biohazard Waste containing box 6 further include have containing box guide rail 605, if It sets on the pedestal 9, the containing box box cover 603 passes through containing box guide-track groove 607 arranged at the bottom and the containing box Guide rail 605 is equipped；The outside of the containing box box cover 603 is additionally provided with handle 604.

As shown in figure 9, there is reaction cup sample introduction mechanism 8 reaction cup box 802, the reaction cup box 802 to pass through sample introduction holder 809 are mounted on the pedestal 9；Reaction cup 801 is assemblied in reaction cup packaging consumptive material 803, is pushed into the reaction cup box 802 It is interior；One end of the reaction cup sample introduction mechanism 8 is additionally provided with sample introduction motor 808, and the sample introduction motor 808 has synchronous pulley 807, in the other end of the reaction cup sample introduction mechanism 8, it is additionally provided with and 807 matched idle pulley 804 of the synchronous pulley；It twines The synchronous belt 806 being wound on the synchronous pulley 808 and the idle pulley 804, that section being located above are placed in the reaction cup In box 802, that underlying section is placed in the outside of the reaction cup box 802；It is additionally provided on the synchronous belt 806 anti- Glass push plate 805 is answered, it is passable as variation, it is both provided with reaction cup push plate in the both sides up and down of synchronous belt, in such manner, it is possible to Save the propulsion time of reaction cup packaging consumptive material.

When specifically used, reaction cup 801 is assemblied in reaction cup packaging consumptive material 803, as shown in figure 9, sample introduction motor 808 is dynamic Make, synchronous pulley 807, synchronous belt 806 is driven to rotate, the reaction cup push plate 805 on synchronous belt 806 is moved, reaction cup packaging consumption Material 803 is manually pushed back into reaction cup packaging consumption material box 811 in reaction cup box 802；Reaction cup 801 is by reaction cup push plate 805 It is pushed into nucleic acid extraction module 1, as shown in Fig. 2, reaction cup 801 is allocated in reaction framework 116 by driving lever 107；Utilize machinery Arm mechanism 3 is originally added in reaction cup 801 from being sampled in sample access mechanism 2, and mechanical arm mechanism 3 is moved along X-axis over the mount 10 It is dynamic, it can adjust X axis position；After reaction, nucleic acid is extracted by mechanical arm mechanism 3 into reagent storing mechanism 7, driving lever 107 Reaction cup waste is pushed in dustbin 104, the reaction cup waste in dustbin 104 passes through reaction cup shown in fig. 7 Waste conveyer 601 is sent in the containing box box cover in Biohazard Waste containing box 6, and side also has head waste of substituting de- Handler 602 drops to substitute head in containing box box cover；Mechanical arm mechanism 3 will be equipped with reagent from reagent storing mechanism 7 again EP pipes extract to PCR modules 5 and be detected, detecting system uses fiber optic conduction technology, and original very large PCR equipment is reduced Extremely among slight PCR modules, while also accomplishing that each sample is individually examined, does not interfere with each other.

The basic principles and main features and advantage of the present invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, such as being mounted on Some adjustment are done in the position of all parts on pedestal, and these changes and improvements all fall within the protetion scope of the claimed invention.This Invention is claimed range and is defined by the appending claims and its equivalent thereof.

Claims (8)

1. a kind of PCR modules for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, which is characterized in that the PCR modules have Cover board is provided with several reagent wells on the cover board；Heat corresponding with the reagent wells is provided with below the cover board Seat is equipped with heating plate and amplification radiator in the bottom of the hot seat；The heating plate is located at the hot seat and the expansion Between increasing radiator, connected with nylon cylinder between amplification radiator and the cover board；The hot seated connection enters to have optical fiber.

2. the PCR modules according to claim 1 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In being provided with conducting wire in the heating plate；The hot seat is made of hollow circuit cylinder hole, laterally prominent in the centre position of the hot seat Go out to have insertion hole, the optical fiber to be accessed in the hot seat by the insertion hole.

3. the PCR modules according to claim 1 or 2 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature It is, 16 reagent wells is provided on the cover board, corresponding, the hot seat also has 16；It is provided with platform in the reagent wells Rank.

4. the PCR modules according to claim 3 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In the amplification radiator includes the upper surface for installing the heating plate；Two opposite sides of the upper surface vertically to Under be extended with the first cooling fin and the second cooling fin；It is additionally provided in several between first cooling fin and the second cooling fin Portion's cooling fin has heat dissipation gap between two neighboring inner fin.

5. the PCR modules according to claim 4 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In the reagent wells are evenly distributed on the cover board；It is provided on the cover board in mating connection with the nylon cylinder Mounting hole.

6. the PCR modules according to claim 5 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In also there is mounting plate, the mounting plate to be directly connected to the heating plate for the bottom of the hot seat.

7. the PCR modules according to claim 6 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In the heating plate is fixed between hot seat and amplification radiator and has thermal insulation board cladding.

8. the PCR modules according to claim 6 for full-automatic nucleic acid extraction amplification diagnosis all-in-one machine, feature exists In the amplification radiator is mounted on each hot seat lower part with the nylon cylinder.