CN212174961U

CN212174961U - Fluorescent quantitative PCR instrument

Info

Publication number: CN212174961U
Application number: CN201921932248.6U
Authority: CN
Inventors: 林华青; 李俊玲; 王国升; 易展飞; 黄晓雨
Original assignee: Zhuhai Dl Medical Biotech Co ltd
Current assignee: Zhuhai deer Bioengineering Co.,Ltd.
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2020-12-18
Anticipated expiration: 2029-11-08

Abstract

The utility model discloses a fluorescence quantitative PCR appearance, including formation of image room, bracket, heating apparatus, imaging device and a plurality of light source. The bottom of the imaging chamber is provided with an opening, the bracket is movably arranged at the opening, and the bracket is used for placing a sample. The heating device is arranged below the bracket and used for heating the sample. The top of the imaging chamber is provided with a first through hole, the plurality of light sources are arranged on the inner wall or the top of the upper end of the imaging chamber around the first through hole, the light of the light sources is towards the opening, and the imaging equipment is arranged above the first through hole. Can conveniently put into the lower extreme of formation of image room with the sample through the bracket in, a plurality of light sources shine simultaneously and form the illuminated area on the bracket for the bracket is received irradiant region and is wideer, and each subregion luminance in illuminated area is more even, can once gather the image information of more quantity samples, and imaging device is directly over at the illuminated area, and the imaging device of being convenient for receives the reverberation of sample, reduces the deformation of sample image.

Description

Fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a DNA detects technical field, in particular to fluorescence quantitative PCR appearance.

Background

The fluorescent quantitative PCR technology is a method of adding fluorescent groups into a PCR reaction system, realizing real-time monitoring of the whole process of PCR through continuous accumulation of fluorescent signals, and then carrying out quantitative analysis on unknown templates through a standard curve. In the real-time fluorescent quantitative PCR, the whole PCR amplification process is detected in real time, a curve can be drawn according to the reaction time and the change of a fluorescent signal, and the fluorescent quantitative PCR detection has important significance for the fields of medicine and food. The existing fluorescence quantitative PCR instrument has the defects that the illumination of a sample is not uniform, and when image information is collected, the sample image far away from the center of a clamping plate is seriously deformed, namely, the phenomena of strong optical layer difference and serious corner effect occur, so that the detection effect of a fluorescence signal is poor, and a large error easily occurs.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a fluorescence quantitative PCR appearance, image information formation of image is good, can once gather the image information of multiunit sample.

According to the utility model discloses a fluorescence quantitative PCR appearance of first aspect embodiment, including formation of image room, bracket, heating apparatus, imaging device and a plurality of light source. The bottom of the imaging chamber is provided with an opening, the bracket is movably arranged at the opening, namely the bracket can extend into the lower end of the imaging chamber, and a sample can be placed on the bracket. The heating device is arranged below the bracket and used for heating the sample. The top of the imaging chamber is provided with a first through hole, the plurality of light sources surround the first through hole and are arranged on the inner wall or the top of the upper end of the imaging chamber, and the light of the light sources is towards the opening. The imaging device is arranged above the first through hole and used for collecting image information of the sample.

According to the utility model discloses fluorescence quantitative PCR appearance has following beneficial effect at least:

when using fluorescence quantitative PCR appearance, can conveniently put into the sample in the lower extreme of formation of image room through the bracket, heating device heats the sample to suitable temperature, thereby carry out PCR and amplify, a plurality of light sources shine to the opening simultaneously, can form the illumination region on the bracket, also be illumination on the sample, make the bracket illuminated region wider, each subregion luminance in illumination region is more even, can once gather the image information of more samples, imaging equipment is in directly over the illumination region, the reverberation of the imaging equipment receipt sample of being convenient for, reduce the deformation of sample image, improve fluorescence detection's effect.

According to some embodiments of the utility model, be equipped with a plurality of detachable cardboard on the bracket, the cardboard is used for placing the sample.

According to some embodiments of the utility model, be equipped with a plurality of collecting pipe on the cardboard, the sample can be placed in the collecting pipe, a plurality of second through-hole has been seted up on the bracket, the collecting pipe can pass the second through-hole extends to the below of bracket.

According to some embodiments of the utility model, the heating apparatus includes the heating seat, a plurality of cell bodies have been seted up on the heating seat, the heating seat is connected with lift drive arrangement, so that the cell body can overlap on the collecting pipe.

According to the utility model discloses a some embodiments still include the base, be equipped with the guide pillar on the base, the cover can be equipped with the guide pin bushing on the guide pillar with sliding, the heating seat with the guide pin bushing is connected.

According to some embodiments of the utility model, lift drive still includes the third connecting rod including articulated first motor, first swing arm, first connecting rod and second connecting rod in proper order, the second connecting rod is kept away from the one end of first connecting rod with the guide pin bushing is connected, the one end of third connecting rod through the hinge simultaneously with first connecting rod with the second connecting rod is articulated, the other end of third connecting rod with the base is articulated.

According to some embodiments of the utility model, still include the slide rail and with slide rail matched with slide plate, the opening with either one of them is equipped with in the bracket the slide rail, another is equipped with the slide plate.

According to some embodiments of the utility model, the bracket connection has the drive arrangement that slides, the drive arrangement that slides includes the second motor, connects the gear of second motor output and with gear matched with rack, the rack with the bracket connection.

According to some embodiments of the utility model, still establish including the lid the hot lid of cardboard top, the hot lid is ground glass, the hot upper surface that covers is the frosting, and the lower surface is the plain noodles.

According to some embodiments of the invention, all the light sources are LED light sources.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic structural diagram of a fluorescence quantitative PCR instrument according to an embodiment of the present invention;

FIG. 2 is a schematic cross-sectional view of the fluorescence quantitative PCR instrument shown in FIG. 1;

FIG. 3 is a schematic top view of the warming means and the elevating driving means of the fluorescence quantitative PCR instrument shown in FIG. 1;

FIG. 4 is a schematic top view of a heating base of the fluorescence quantitative PCR instrument shown in FIG. 1.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, a plurality of means are one or more, and a plurality of means are two or more. In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.

Referring to fig. 1 and 2, a fluorescence quantitative PCR instrument includes an imaging chamber 100, a carriage 200, a warming device 300, an imaging apparatus 500, and a plurality of light sources 400. The bottom of the imaging chamber 100 is provided with an opening, and the bracket 200 is movably disposed at the opening, that is, the bracket 200 can extend into the lower end of the imaging chamber 100, and the sample can be placed on the bracket 200. The warming device 300 is provided below the carriage 200, and the warming device 300 is used to warm the specimen. The top of the imaging chamber 100 is opened with a first through hole 110, a plurality of light sources 400 are arranged on the top of the imaging chamber 100 around the first through hole 110, and light from the light sources 400 is directed to the opening. The imaging device 500 is disposed above the first through hole 110, and the imaging device 500 is used for acquiring image information of the sample.

In another embodiment, a plurality of light sources 400 are disposed around the first through hole 110 on the inner wall of the upper end of the imaging chamber 100, and the light from the light sources 400 is irradiated onto the bracket 200 at the opening.

When using fluorescence quantitative PCR appearance, can conveniently put into the sample in the lower extreme of formation of image room 100 through bracket 200, heating apparatus 300 heats the sample to suitable temperature, thereby carry out the PCR amplification, a plurality of light sources 400 shine to the opening simultaneously, form the photic region on cardboard 210, also be on the sample, make the photic region wideer, each subregion luminance in photic region is more even, can once gather the image information of more quantity samples, imaging apparatus 500 is directly over the photic region, be convenient for imaging apparatus 500 receives the reverberation of sample, reduce the deformation of sample image, it is effectual to image, improve fluorescence detection's effect.

In the embodiment of the present invention, the light sources 400 are four in total, and the four light sources 400 are uniformly distributed at the top of the first through hole 110, so that the range of the bracket 200 illuminated by light is wider, the light is more uniform, and more clamping plates 210 can be placed.

In some embodiments of the present invention, the tray 200 is provided with a plurality of detachable clamping plates 210, and the clamping plates 210 are used for placing samples. After all samples are placed on the clamping plate 210, the clamping plate 210 can be clamped on the bracket 200 at one time, the samples can be conveniently placed at the lower end in the imaging chamber 100, and it can be understood that the samples can be also taken out quickly and conveniently.

The utility model discloses a some embodiments are equipped with a plurality of collecting pipe 211 on the cardboard 210, and the sample can be placed in collecting pipe 211, has seted up a plurality of second through-hole on the bracket 200, and collecting pipe 211 can pass the second through-hole and extend to the below of bracket 200, when needs are placed or are taken out the sample, can directly inject cardboard 210 and collecting pipe 211 from the second through-hole or dial out in the second through-hole, and is swift convenient to realize cardboard 210's the function of dismantling. In this embodiment, the number of the clamping plates 210 and the number of the second through holes are four, and the number of the collecting tubes 211 on each clamping plate 210 is thirty-six, so that the fluorescence quantitative PCR instrument can measure and monitor a large number of samples at a time.

In some embodiments of the present invention, the heating apparatus 300 comprises a heating base 310, and the heating base 310 is connected to a lifting driving device 900. Referring to fig. 4, the heating base 310 is provided with a plurality of grooves 311, the grooves 311 can be sleeved on the collecting pipe 211, the inner wall of the groove 311 is provided with heating wires, and the heating wires are electrically connected with a switch and a power supply in sequence. The heating seat 310 is also internally provided with a temperature sensor which is connected with a controller, the controller is electrically connected with a switch, the temperature sensor feeds a temperature signal back to the controller, and the controller compares the preset temperature with the temperature fed back by the temperature sensor, so that the on-off of the electric heating wire is controlled by the switch. When the sample in the collecting pipe 211 needs to be heated, the lifting driving device 900 drives the heating seat 310 to ascend, so that the groove body 311 is sleeved on the collecting pipe 211, the groove body 311 can quickly and uniformly heat the sample in the collecting pipe 211, and when the heating is not needed, the lifting driving device 900 drives the heating seat 310 to descend to be far away from the collecting pipe 211. Further, the lower surface of the heating base 310 is provided with a heat sink 1000, a fan 1500 is disposed below the heat sink 1000, and the fan 1500 can accelerate air flowing through the heat sink 1000, so as to take away heat of the heat sink 1000 and the heating base 310.

In another embodiment, the warming device 300 includes a heating seat 310, a liquid tank is disposed on the heating seat 310, a heating wire is disposed at the bottom of the liquid tank, a liquid, such as water, is heated in the liquid tank, the lower end of the collecting pipe 211 extends into the liquid, the heating wire heats the liquid, the heat of the liquid is uniformly transferred to the collecting pipe 211, and uneven heating of the sample is avoided.

In the embodiment of the fluorescence quantitative PCR instrument, the fluorescence quantitative PCR instrument further comprises a base 700, a guide pillar 1200 is arranged on the base 700, a guide sleeve 1100 is sleeved on the guide pillar 1200 in a sliding manner, the heating seat 310 is connected with the guide sleeve 1100, and the guide pillar 1200 and the guide sleeve 1100 play a guiding role, so that the situation that the collecting pipe 211 cannot be correctly sleeved with the groove body 311 is avoided. Further, the guide pillars 1200 and the guide sleeves 1100 are respectively provided with two guide pillars 1200, the two guide pillars 1200 are respectively arranged at the left end and the right end of the base 700, and the two ends of the heating base 310 are respectively connected with the two guide sleeves 1100, so that the heating base 310 can be lifted more stably.

Specifically, the base 700 includes a bottom plate and two side plates respectively disposed at two ends of the bottom plate, the imaging chamber 100 is disposed at upper ends of the two side plates, the lifting driving device 900 includes a first motor 910, a first swing arm 920, a first link 930, a second link 940, and a third link 950, the second link 940 is connected to the guide sleeve 1100 at an end away from the first link 930, one end of the third link 950 is hinged to the first link 930 and the second link 940 through a hinge shaft, and the other end of the third link 950 is hinged to the base 700. The first motor 910 is disposed on the bottom plate, when the first motor 910 is started, the first motor 910 can drive the first swing arm 920 to swing, and since the second link 940 is hinged to the guide sleeve 1100 and the third link 950 is hinged to the base 700, more specifically, the third link 950 is hinged to the side plate, the hinge point of the second link 940 and the guide sleeve 1100 is above the hinge point of the third link 950 and the side plate, the first swing arm 920 can drive the guide sleeve 1100 to ascend or descend through the first link 930 and the second link 940.

In this embodiment, there are two first swing arms 920, two first connecting rods 930, two second connecting rods 940 and two third connecting rods 950, the first motor 910 is disposed on the bottom plate, bearing seats are disposed at the left and right ends of the bottom plate, a rotating shaft is disposed on the two bearing seats in a straddling manner, the rotating shaft is connected to the first motor 910 through a gear, the two first swing arms 920 are respectively connected to the two ends of the rotating shaft, one first swing arm 920, one first connecting rod 930, one second connecting rod 940 and one third connecting rod 950 form a set of transmission pairs, and the two second connecting rods 940 are respectively connected to the two guide sleeves 1100. The two guide sleeves 1100 are stressed simultaneously, so that the guide column 1200 and the heating base 310 are stressed in a balanced manner, and the structure is more reliable.

In another embodiment, the lifting driving device 900 may be an air cylinder, an output end of the air cylinder is connected to the guide sleeve 1100 or the heating base 310, and in still another embodiment, the lifting driving device 900 may include a motor, a lead screw and a lead screw nut, the motor is connected to the lead screw, the lead screw nut is disposed on the lead screw, and the guide sleeve 1100 is connected to the lead screw nut.

In some embodiments of the present invention, referring to fig. 3, the present invention further comprises a sliding rail 1300 and a sliding plate 1400 matching with the sliding rail 1300, wherein one of the sliding rail 1300 and the sliding plate 1400 is disposed in the opening and the bracket 200, and the other sliding plate 1400 is disposed in the opening and the bracket 200, so that the bracket 200 can extend into or out of the lower end of the imaging chamber 100, thereby facilitating the placement of the sample or the taking out of the sample.

In some embodiments of the present invention, the bracket 200 is connected to a sliding driving device 800, and the sliding driving device 800 drives the bracket 200 to slide back and forth on the sliding rail 1300, so that the bracket 200 extends into or out of the lower end inside the imaging chamber 100. When the bracket 200 extends out of the imaging chamber 100, a sample can be placed on the clamping plate 210, after the sample is placed, the sliding driving device 800 drives the bracket 200 to extend into the lower end of the interior of the imaging chamber 100, and the imaging device shoots an image of the sample. The utility model discloses in, slide rail 1300 and slide 1400 all are equipped with two, and two slide rails 1300 distribute in the left and right sides of formation of image room 100, and two slide rails 1300 set up and are parallel to each other along the fore-and-aft direction, and the both ends of bracket 200 are connected with two slides 1400 respectively for bracket 200 both ends atress is balanced, and is more steady in the time of sliding.

Referring to fig. 1 and 2 again, the sliding driving device 800 includes a second motor 810, a gear 820 connected to an output end of the second motor 810, and a rack 830 engaged with the gear 820, the rack 830 is connected to the bracket 200, and the second motor 810 is disposed on one of the side plates of the base. When the second motor 810 rotates, the bracket 200 can be driven to slide on the slide rail 1300, and the sliding driving device 800 has a simple structure and is convenient to control.

In some embodiments of the utility model, still including covering the hot lid 600 of establishing above cardboard 210, hot lid 600 is ground glass, and hot lid 600 upper surface is the frosting, and the lower surface is the plain noodles, and hot lid 600 can be so that the light that passes hot lid 600 throws more evenly on the cardboard 210 of bracket 200, avoids appearing the noise point on cardboard 210, also avoids appearing the especially bright spot region of illumination luminance on cardboard 210.

The utility model discloses an in some embodiments, bracket 200 upper surface is equipped with round heat preservation cotton, and the heat preservation cotton is in the middle of circling cardboard 210, and hot lid 600 is established on the heat preservation cotton, and the cotton thermal loss of avoiding of heat that keeps warm avoids the sample cooling.

In some embodiments of the utility model, all light sources 400 are the LED light source, and the LED light source has luminance height, the optional characteristic of wavelength range for imaging device's the effect of shooing is better. An optical filter can be arranged below the light source 400, and light with the most appropriate wavelength is selected to illuminate the sample, so that the photographing effect is better. The imaging device adopts a CMOS type camera, the imaging and photographing effects are better, the imaging device is connected with a computer, and the computer analyzes image information returned by the imaging device and displays the result.

The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the gist of the present invention within the knowledge range of those skilled in the art.

Claims

1. A fluorescent quantitative PCR instrument for measuring a sample, comprising:

the imaging chamber (100) is provided with a first through hole (110) at the top and an opening at the bottom;

the bracket (200) is movably arranged at the opening and used for placing the sample;

a warming device (300) arranged below the bracket (200) and used for warming the sample;

a plurality of light sources (400) arranged on the inner wall or the top of the upper end of the imaging chamber (100) around the first through hole (110), wherein the light of the light sources (400) is irradiated to the opening;

and the imaging device (500) is arranged above the first through hole (110) and is used for acquiring the image information of the sample.

2. The fluorescence quantitative PCR instrument according to claim 1, characterized in that: the bracket (200) is provided with a plurality of detachable clamping plates (210), and the clamping plates (210) are used for placing the samples.

3. The fluorescence quantitative PCR instrument according to claim 2, characterized in that: the sample collection device is characterized in that the clamping plate (210) is provided with a plurality of collection pipes (211), samples can be placed in the collection pipes (211), the bracket (200) is provided with a plurality of second through holes, and the collection pipes (211) can penetrate through the second through holes and extend to the lower side of the bracket (200).

4. The fluorescence quantitative PCR instrument according to claim 3, wherein: the heating device (300) comprises a heating seat (310), a plurality of groove bodies (311) are arranged on the heating seat (310), and the heating seat (310) is connected with a lifting driving device (900) so that the groove bodies (311) can be sleeved on the collecting pipe (211).

5. The fluorescence quantitative PCR instrument according to claim 4, wherein: the heating device is characterized by further comprising a base (700), wherein a guide pillar (1200) is arranged on the base (700), a guide sleeve (1100) is sleeved on the guide pillar (1200) in a sliding manner, and the heating seat (310) is connected with the guide sleeve (1100).

6. The fluorescence quantitative PCR instrument according to claim 5, wherein: the lifting driving device (900) comprises a first motor (910), a first swing arm (920), a first connecting rod (930), a second connecting rod (940) and a third connecting rod (950), wherein the first motor (910), the first swing arm (920), the first connecting rod (930) and the second connecting rod (940) are sequentially hinged, one end, far away from the first connecting rod (930), of the second connecting rod (940) is connected with the guide sleeve (1100), one end of the third connecting rod (950) is hinged with the first connecting rod (930) and the second connecting rod (940) through a hinge shaft, and the other end of the third connecting rod (950) is hinged with the base (700).

7. The fluorescence quantitative PCR instrument according to any one of claims 1 to 6, characterized in that: the sliding plate type sliding rack is characterized by further comprising a sliding rail (1300) and a sliding plate (1400) matched with the sliding rail (1300), wherein one of the opening and the bracket (200) is provided with the sliding rail (1300), and the other one is provided with the sliding plate (1400).

8. The fluorescence quantitative PCR instrument according to claim 7, wherein: the sliding driving device (800) is connected to the bracket (200), the sliding driving device (800) comprises a second motor (810), a gear (820) connected with the output end of the second motor (810) and a rack (830) matched with the gear (820), and the rack (830) is connected with the bracket (200).

9. The fluorescence quantitative PCR instrument according to any one of claims 2 to 6, characterized in that: the polishing device is characterized by further comprising a hot cover (600) covering the clamping plate (210), wherein the hot cover (600) is made of ground glass, the upper surface of the hot cover (600) is a frosted surface, and the lower surface of the hot cover is a smooth surface.

10. The fluorescence quantitative PCR instrument according to any one of claims 1 to 6, characterized in that: all the light sources (400) are LED light sources.