JP2624719B2 - Micro injection device - Google Patents
Micro injection deviceInfo
- Publication number
- JP2624719B2 JP2624719B2 JP62270197A JP27019787A JP2624719B2 JP 2624719 B2 JP2624719 B2 JP 2624719B2 JP 62270197 A JP62270197 A JP 62270197A JP 27019787 A JP27019787 A JP 27019787A JP 2624719 B2 JP2624719 B2 JP 2624719B2
- Authority
- JP
- Japan
- Prior art keywords
- cells
- cell
- needle
- fixing plate
- injection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は植物、微生物、動物等の細胞に物質を注入す
るマイクロインジエクシヨンの高効率化に関するもので
ある。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a high efficiency microinjection for injecting a substance into cells of plants, microorganisms, animals and the like.
従来、機械式マイクロインジエクシヨンは、プラント
・セル・フイジオロジー、26（２）（1985年）第229頁
から第236頁（Plant Cell Physiology.26（２）PP229〜
236、（1985））において記載されている手法で行なわ
れていた。この方法では、短時間に多量の細胞を処理す
るのは困難である。Conventionally, mechanical microinjection is described in Plant Cell Physiology, 26 (2) (1985) pp. 229 to 236 (Plant Cell Physiology. 26 (2) PP229-
236, (1985)). With this method, it is difficult to treat a large number of cells in a short time.
微小注射針を細胞に差し込んで物質を注入する機械式
マイクロインジエクシヨン法は、マニピユレーターを用
い顕微鏡下で１個１個の細胞を処理していくもので、高
度の熟練を要し処理能力が限られていた。The mechanical microinjection method, in which a substance is injected by inserting a microinjection needle into cells, uses a manipulator to process each cell under a microscope, requiring a high degree of skill. The ability was limited.
本発明の目的は、短時間に多量の細胞の処理ができる
自動化可能な方法を提供することにある。An object of the present invention is to provide an automatable method capable of treating a large amount of cells in a short time.
上記目的は、平板上に二次元状に規則正しく配列する
ように設けられた保持点に細胞を１個１個配置し、さら
に、配置した細胞との位置関係を制御する機構を有する
注射針を用いてマイクロインジエクシヨンを逐次行うこ
とにより達成される。The above object is to use an injection needle having a mechanism for arranging cells one by one at holding points provided so as to be regularly arranged two-dimensionally on a flat plate and further controlling a positional relationship with the arranged cells. This is achieved by sequentially performing microinjection.
所定の平板の保持点に細胞を規則正しく二次元状に配
列させることによつて個々の細胞にアドレスをつけるこ
とができる。次に制御機構を有する針によつて、そのア
ドレスで決められた位置に次々とマイクロインジエクシ
ヨンを行つてゆく。これらの機構によつて、連続的かつ
自動的にマイクロインジエクシヨンを行つていける。By arranging the cells regularly and two-dimensionally at the holding points of a predetermined plate, it is possible to address individual cells. Next, the microinjection is performed one after another at a position determined by the address by a needle having a control mechanism. These mechanisms enable continuous and automatic microinjection.
第１図は本発明の実施例になる装置の全体概略斜視図
である。本装置では、第２図に断面図を示す如く、規則
正しく配列し、かつ細胞径よりも小さな孔を有する細胞
固定板11を、吸引器４に固定し、パイプ７で結合された
吸引ポンプ６により吸引することによつて細胞固定板11
の上に細胞10を規則正しく配列させる。FIG. 1 is an overall schematic perspective view of an apparatus according to an embodiment of the present invention. In this apparatus, as shown in the cross-sectional view of FIG. 2, a cell fixing plate 11 having a regular array and having holes smaller than the cell diameter is fixed to the suction device 4 and is suctioned by a suction pump 6 connected by a pipe 7. The cell fixation plate 11 is aspirated by suction.
The cells 10 are regularly arranged on the top.
細胞の固定法としては、この他にも第３図の様に細胞
固定板16を絶縁体で作成し、そこに微小電極13を規則正
しく設置し、電極15との間に高周波電源14によつて高周
波を印加することによつて、細胞に動く電場の力を利用
して細胞10を固定する方法がある。また、第４図のよう
に、細胞に親和性のない、例えばSiでできた細胞固定板
17に、例えばSiO2の様な細胞親和性物質18を規則正しく
設置することによつても細胞10を固定板11上に規則正し
く配列し、固定できる。なおこの場合、細胞との親和力
は、細胞固定板＜細胞親和性物質となつていれば良い。As another method for fixing the cells, as shown in FIG. 3, a cell fixing plate 16 is made of an insulator, and microelectrodes 13 are regularly placed there. There is a method in which the cell 10 is fixed by applying a high frequency and using the force of an electric field moving on the cell. In addition, as shown in FIG. 4, a cell fixing plate made of, for example, Si having no affinity for cells.
The cells 10 can also be regularly arranged on the fixing plate 11 and fixed by arranging the cell-affinity substances 18 such as SiO 2 regularly on the fixing plate 17. In this case, the affinity with the cells may be such that the cell fixation plate <the cell affinity substance.
細胞が規則正しく配列固定されたら、続いて注入針２
を細胞に差し込む。注入針の位置は、Ｘ−Ｙ制御台８及
びコンピユーター３によつて、制御される。細胞内にお
ける針先の位置については、細胞固定板の面内方向は、
上記のＸ−Ｙ制御台８とコンピユーター３によつて決め
られ、板面に垂直な方向は、針昇降装置５及びコンピユ
ーターによつて制御される。なお板面に垂直な方向は、
板面を基準に制御する。物質の注入量は、注入ポンプ１
及びコンピユーター３によつて制御される。細胞への物
質の注入終了後は、針昇降装置５によつて針２は細胞か
ら抜かれる。続いて針昇降装置５、及び針２はＸ−Ｙ制
御台８によつて移動し、次の目標とする細胞に対してイ
ンジエクシヨンを施すのに丁度良い位置で停止する。以
上の注入終了から移動終了までの一連の動作は、コンピ
ユーター３によつて制御される。固定された細胞個々に
対して、以上で述べてきた注入の動作を繰り返す。な
お、細胞固定板11（もしくは16,17）とＸ−Ｙ制御台８
との位置関係は、結合部９によつて厳密に決められてい
る。注入効率をさらに向上させるために注入針２を複数
個用いることもできる。After the cells are regularly arranged and fixed, the injection needle 2
Into the cells. The position of the injection needle is controlled by the XY control table 8 and the computer 3. Regarding the position of the needle tip in the cell, the in-plane direction of the cell fixation plate
The direction perpendicular to the plate surface, which is determined by the XY control table 8 and the computer 3, is controlled by the needle elevating device 5 and the computer. The direction perpendicular to the plate surface is
Control based on the plate surface. The injection amount of the substance is determined by the injection pump 1
And is controlled by the computer 3. After the injection of the substance into the cell is completed, the needle 2 is removed from the cell by the needle elevating device 5. Subsequently, the needle raising / lowering device 5 and the needle 2 are moved by the XY control table 8 and stopped at a position which is just enough to apply the indices to the next target cell. A series of operations from the end of the injection to the end of the movement are controlled by the computer 3. The injection operation described above is repeated for each fixed cell. The cell fixing plate 11 (or 16, 17) and the XY control table 8
Is strictly determined by the coupling unit 9. In order to further improve the injection efficiency, a plurality of injection needles 2 can be used.
本実施例によれば、短時間に多数の細胞にマイクロイ
ンジエクシヨンを施することができる。According to this embodiment, microinjection can be applied to a large number of cells in a short time.
本発明によれば、複数個の細胞を２次元状に規則正し
く配列固定し、細胞に物質を注入することができ、細胞
に対する注入針の位置を制御できるので、短時間に多数
の細胞を処理することが可能となり、自動化が容易にで
きる。According to the present invention, a plurality of cells are regularly arranged and fixed two-dimensionally, a substance can be injected into the cells, and the position of the injection needle with respect to the cells can be controlled, so that a large number of cells can be processed in a short time. And automation can be easily performed.
第１図は本発明の一実施例を示す概略斜視図、第２図は
細胞固定部の拡大側断面図、第３図，第４図は細胞を配
列固定する手段の他の実施例を示す側断面図である。 １……注入ポンプ、２……注入針、３……コンピユータ
ー、４……吸引器、５……針昇降装置、６……吸引ポン
プ、７……パイプ、８……Ｘ−Ｙ制御台、９……支持
棒、10……細胞、11……細胞固定板Ａ、12……コード、
13……微小電極、14……高周波電源、15……電極、16…
…細胞固定板Ｂ、17……細胞固定板Ｃ、18……細胞親和
物質。FIG. 1 is a schematic perspective view showing one embodiment of the present invention, FIG. 2 is an enlarged sectional side view of a cell fixing portion, and FIGS. 3 and 4 show another embodiment of means for arranging and fixing cells. It is a side sectional view. DESCRIPTION OF SYMBOLS 1 ... Infusion pump, 2 ... Infusion needle, 3 ... Computer, 4 ... Aspirator, 5 ... Needle elevating device, 6 ... Suction pump, 7 ... Pipe, 8 ... XY control stand, 9 ... support rod, 10 ... cells, 11 ... cell fixing plate A, 12 ... cord,
13 ... Microelectrode, 14 ... High frequency power supply, 15 ... Electrode, 16 ...
... cell fixing plate B, 17 ... cell fixing plate C, 18 ... cell affinity substance.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 佐藤 一雄 東京都国分寺市東恋ケ窪１丁目280番地 株式会社日立製作所中央研究所内 (56)参考文献 特開 昭61−265284（ＪＰ，Ａ) 実開 昭61−182299（ＪＰ，Ｕ) ──────────────────────────────────────────────────続 き Continuation of the front page (72) Inventor Kazuo Sato 1-280 Higashi Koigakubo, Kokubunji-shi, Tokyo Inside the Central Research Laboratory, Hitachi, Ltd. (56) References JP-A-61-265284 (JP, A) −182299 (JP, U)
Claims (1)
機械式マイクロインジェクション装置において、複数の
細胞を２次元状に規則正しく配列・固定する細胞固定板
と、上記細胞固定板に対して上記針の位置関係を制御す
る手段とを備えたことを特徴とするマイクロインジェク
ション装置。1. A mechanical microinjection device for injecting a substance into cells by means of a minute needle, a cell fixing plate for regularly arranging and fixing a plurality of cells in a two-dimensional manner, and said needle for said cell fixing plate. Means for controlling the positional relationship between the microinjection device and the microinjection device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62270197A JP2624719B2 (en) | 1987-10-28 | 1987-10-28 | Micro injection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62270197A JP2624719B2 (en) | 1987-10-28 | 1987-10-28 | Micro injection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01112976A JPH01112976A (en) | 1989-05-01 |
| JP2624719B2 true JP2624719B2 (en) | 1997-06-25 |
Family
ID=17482880
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62270197A Expired - Lifetime JP2624719B2 (en) | 1987-10-28 | 1987-10-28 | Micro injection device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2624719B2 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1595941A2 (en) * | 2004-05-10 | 2005-11-16 | Fujitsu Limited | Microinjectiuon device and microinjection method |
| EP1693444A1 (en) | 2005-02-17 | 2006-08-23 | Fujitsu Limited | Substance injecting apparatus |
| EP1752221A1 (en) | 2005-08-05 | 2007-02-14 | Fujitsu Limited | Automatic microinjection apparatus and cell trapping plate |
| WO2010079580A1 (en) | 2009-01-09 | 2010-07-15 | Ntn株式会社 | Microinjection apparatus and microinjection method |
| US7881533B2 (en) | 2006-07-28 | 2011-02-01 | Fujitsu Limited | Microinjection apparatus and microinjection method |
| US7897395B2 (en) | 2007-04-27 | 2011-03-01 | Fujitsu Limited | Microinjection apparatus, trap plate and microinjection method |
| US7936939B2 (en) | 2006-06-29 | 2011-05-03 | Fujitsu Limited | Microinjection apparatus and automatic focal point adjustment method |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5262128A (en) * | 1989-10-23 | 1993-11-16 | The United States Of America As Represented By The Department Of Health And Human Services | Array-type multiple cell injector |
| JPH03172166A (en) * | 1989-11-30 | 1991-07-25 | Shimadzu Corp | Apparatus for introducing gene |
| US5173158A (en) * | 1991-07-22 | 1992-12-22 | Schmukler Robert E | Apparatus and methods for electroporation and electrofusion |
| JP3442357B2 (en) | 2000-08-25 | 2003-09-02 | 株式会社日立製作所 | Amphibian oocyte sample introduction device, amphibian oocyte sample introduction system, amphibian oocyte sample introduction method, amphibian oocyte production method, amphibian oocyte and method of selling or transferring it, as sensor for screening Method used, container, and analysis method |
| WO2004092369A1 (en) | 2003-04-11 | 2004-10-28 | Riken | Method of microinjection and device therefor |
| JP4504082B2 (en) | 2004-04-28 | 2010-07-14 | 富士通株式会社 | Liquid injection device |
| JP4555650B2 (en) * | 2004-09-29 | 2010-10-06 | 富士通株式会社 | Cell supply / discharge / capture apparatus and cell supply / discharge / capture method |
| JP2006149226A (en) * | 2004-11-25 | 2006-06-15 | Fujitsu Ltd | Apparatus and method for capturing minute object |
| KR100712603B1 (en) * | 2006-04-18 | 2007-05-02 | 김병진 | Working block for removal of a nucleus from an egg |
| JP2008022805A (en) * | 2006-07-24 | 2008-02-07 | Fujitsu Ltd | Apparatus and method for sucking or ejecting substance |
| JP5257276B2 (en) * | 2009-07-01 | 2013-08-07 | 日本精工株式会社 | Manipulation system drive method |
| JP2011205982A (en) * | 2010-03-30 | 2011-10-20 | Ntn Corp | Microinjection device |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0436636Y2 (en) * | 1985-05-01 | 1992-08-28 | ||
| JPS61265284A (en) * | 1985-12-26 | 1986-11-25 | 株式会社 成茂科学器械研究所 | Manipulator for glass electrode,etc. |
-
1987
- 1987-10-28 JP JP62270197A patent/JP2624719B2/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1595941A2 (en) * | 2004-05-10 | 2005-11-16 | Fujitsu Limited | Microinjectiuon device and microinjection method |
| EP1595941A3 (en) * | 2004-05-10 | 2007-02-14 | Fujitsu Limited | Microinjectiuon device and microinjection method |
| EP1693444A1 (en) | 2005-02-17 | 2006-08-23 | Fujitsu Limited | Substance injecting apparatus |
| EP1752221A1 (en) | 2005-08-05 | 2007-02-14 | Fujitsu Limited | Automatic microinjection apparatus and cell trapping plate |
| US7936939B2 (en) | 2006-06-29 | 2011-05-03 | Fujitsu Limited | Microinjection apparatus and automatic focal point adjustment method |
| US7881533B2 (en) | 2006-07-28 | 2011-02-01 | Fujitsu Limited | Microinjection apparatus and microinjection method |
| US7897395B2 (en) | 2007-04-27 | 2011-03-01 | Fujitsu Limited | Microinjection apparatus, trap plate and microinjection method |
| WO2010079580A1 (en) | 2009-01-09 | 2010-07-15 | Ntn株式会社 | Microinjection apparatus and microinjection method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH01112976A (en) | 1989-05-01 |
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