KR100712603B1 - Working block for removal of a nucleus from an egg - Google Patents

Working block for removal of a nucleus from an egg Download PDF

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Publication number
KR100712603B1
KR100712603B1 KR1020060034811A KR20060034811A KR100712603B1 KR 100712603 B1 KR100712603 B1 KR 100712603B1 KR 1020060034811 A KR1020060034811 A KR 1020060034811A KR 20060034811 A KR20060034811 A KR 20060034811A KR 100712603 B1 KR100712603 B1 KR 100712603B1
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South Korea
Prior art keywords
egg
block
nucleus
groove
removal
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KR1020060034811A
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Korean (ko)
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김병진
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김병진
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Priority to KR1020060034811A priority Critical patent/KR100712603B1/en
Priority to PCT/KR2006/002559 priority patent/WO2007119903A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells

Abstract

본 발명은 생명공학 분야에서 난자를 이용한 연구에 활용되기 위한 작업블럭에 대한 것으로, 특히 블럭몸체의 일측에 난자의 외형과 일치하는 개방형 또는 밀폐형 요홈부를 형성하고, 블럭몸체의 타측에는 상기 요홈부와 관통되는 마이크로 니들 삽입공을 형성함으로서,The present invention relates to a working block for use in research using the egg in the field of biotechnology, in particular, on one side of the block body to form an open or closed groove to match the appearance of the egg, the other side of the block body and the groove By forming the penetrating micro needle insertion hole,

난자의 핵 제거 및 이식을 하고자 하는 경우 상기 요홈부에 의해 난자가 요동하지 않게 되므로 구멍의 형성 및 핵 제거 과정이 보다 빠르고 안정적으로 이루어짐은 물론 그에 따른 불량률을 최소화하여 연구의 효율성 및 성과를 향상시키는 특징을 갖는 난자의 핵 제거용 작업블럭에 대한 것이다.When the egg is to be removed and transplanted, the groove is not shaken by the groove so that the hole formation and nucleation process are faster and more stable, and the defect rate is minimized, thereby improving the efficiency and performance of the research. The present invention relates to an operation block for nucleation of eggs having characteristics.

난자, 작업블럭, 요홈부, 마이크로 피펫, 삽입공, 핵 제거, 생명공학 Ov, work block, groove, micro pipette, insertion hole, nucleation, biotechnology

Description

난자의 핵 제거용 작업블럭 {Working block for removal of a nucleus from an egg}Working block for removal of a nucleus from an egg}

도 1은 본 발명에 따른 난자의 핵 제거용 작업블럭의 전체사시도1 is an overall perspective view of the operation block for removing the nucleus of the egg according to the present invention;

도 2는 본 발명에 따른 난자의 핵 제거용 작업블럭의 또다른 실시예를 보인 사시도Figure 2 is a perspective view showing another embodiment of the operation block for nucleus removal of the egg according to the present invention

도 3은 본 발명에 따른 난자의 핵 제거용 작업블럭을 이용한 핵 제거 과정을 보인 평단면도Figure 3 is a cross-sectional view showing a nucleation process using the nucleus removal operation block of the egg according to the present invention.

도 4a 및 도 4b는 본 발명에 따른 난자의 핵 제거용 작업블럭의 단부 실시예를 보인 단면도로서,4A and 4B are cross-sectional views illustrating an end embodiment of an operation block for removing a nucleus of an egg according to the present invention;

도 4a는 반개방형 단부 구조를 갖는 단수 작업블럭의 요부 확대도4A is an enlarged view of a main portion of a singular work block having a semi-open end structure;

도 4b는 반밀폐형 단부 구조를 갖는 단수 작업블럭의 요부 확대도4b is an enlarged view of a main portion of a single work block having a semi-sealed end structure;

도 5a 및 도 5b는 본 발명에 따른 난자의 핵 제거용 작업블럭의 또다른 실시예도로서,5a and 5b is another embodiment of the work block for nucleus removal of the egg according to the present invention,

도 5a는 반개방형 단부 구조를 갖는 복수 작업블럭의 요부 확대도5A is an enlarged view of a main portion of a multi-task block having a semi-open end structure;

도 5b는 반밀폐형 단부 구조를 갖는 복수 작업블럭의 요부 확대도5b is an enlarged view of a main portion of a multi-task block having a semi-sealed end structure;

도 6a 및 도 6b는 본 발명에 따른 난자의 핵 제거용 작업블럭의 또다른 실시 예도로서,6a and 6b is another embodiment of the work block for nucleus removal of the egg according to the present invention,

도 6a는 점진 확대되는 반개방형 단부 구조를 갖는 복수 작업블럭의 요부 확대도6A is an enlarged view of a main portion of a multi-task block having a semi-opened end structure gradually expanding;

도 6b는 점진 확대되는 반밀폐형 단부 구조를 갖는 복수 작업블럭의 요부 확대도6B is an enlarged view of a main portion of a multi-task block having a semi-sealed end structure gradually expanding;

* 도면의 주요부분에 대한 부호의 설명 *Explanation of symbols on the main parts of the drawings

1 : 블럭몸체 2 : 요홈부1: block body 2: groove

3 : 삽입공3: insertion hole

10 : 난자 11 : 마이크로 피펫10: egg 11: micropipette

12 : 마이크로 니들12: micro needle

본 발명은 생명공학 분야에서 난자를 이용한 연구에 활용되기 위한 작업블럭에 대한 것으로, 더욱 상세하게는 난자의 핵 제거시 그 난자의 안정적인 안치 및 마이크로 니들(Micro needle)의 유도 삽입, 마이크로 피펫(Micro pipet)을 이용한 핵 제거 등을 편리하게 수행할 수 있게 한 난자의 핵 제거용 작업블럭에 관한 것이다.The present invention relates to a working block for use in research using eggs in the field of biotechnology, and more particularly, stable insertion of the egg and induction insertion of a micro needle (Micro needle), micro pipette (Micro The present invention relates to an operation block for nucleus removal of an egg that allows for convenient nuclear removal using a pipet).

근자에 들어 생명공학 분야에서는 난자를 이용한 다양한 실험이 시행되고 있는데, 난자의 핵을 제거하여 새로운 핵을 이식하는 방법 등을 통해 유전자 조작 및 복제 등의 실험이 이루어지고 있으며, 이러한 일련의 실험은 인류의 건강과 행복을 추구하고자 함에 있다.In recent years, various experiments using eggs have been conducted in the field of biotechnology, and experiments such as genetic manipulation and cloning have been carried out by removing an egg's nucleus and transplanting a new nucleus. In pursuit of health and happiness.

이와 같은 난자를 이용한 생명공학 분야에서의 실험 단계에서 널리 사용되는 도구로는 마이크로 피펫(Micro pipet)이 이용되는데, 상기한 마이크로 피펫(Micro pipet)은 극소량의 액체 등을 계량하거나 옮기는데 쓰이는 작은 관으로서 난자의 핵을 제거하고자 하는 경우 그 난자가 움직이지 못하도록 하는 도구로도 사용된다.As a widely used tool in the experimental stage in the field of biotechnology using such an egg, a micro pipet is used. The micro pipet is a small tube used to measure or transfer a very small amount of liquid. If you want to remove the nucleus of an egg, it is also used as a tool to prevent the egg from moving.

이러한 마이크로 피펫을 이용하여 난자의 핵을 제거하기 위해서는 우선 난자에 마이크로 니들을 이용하여 구멍을 내고, 그 마이크로 피펫과 마이크로 니들의 사이에 난자를 위치시켜 마이크로 피펫을 고정대로 삼아 이들을 서로 가압하여 그 압력에 의해 난자의 핵이 구멍을 통해 제거되게 한 이른바 젓가락 기술을 이용한 방법이 실용화되어 있다.In order to remove the nucleus of an egg by using such a micropipette, first, a hole is made by using a microneedle in the egg, and an egg is placed between the micropipette and the microneedle, and the micropipette is held as a fixation and the pressure is applied to each other. A method using a so-called chopsticks technique, in which the nucleus of an egg is removed through a hole, has been put into practical use.

그러나, 난자는 구형(球型)이면서도 매우 말랑말랑한 성질을 갖고 있어, 상기와 같은 마이크로 피펫(Micro pipet)과 마이크로 니들(Micro needle)을 이용한 난자의 핵 제거시 매우 어려운 작업이 되고 있는 것인데, 상기와 같은 마이크로 피펫과 마이크로 니들을 이용하여 난자를 압압하게 되면, 말랑말랑한 난자는 이리저리 움직이게 되므로 신속하고 효율적인 핵 제거작업이 이루어지지 않게 된다.However, since the egg is spherical and very soft, it is very difficult to remove the nucleus of the egg using a micro pipet and a micro needle as described above. When the oocyte is pressed using a micro pipette and a microneedle, the soft egg moves around, so that a quick and efficient nuclear removal is not performed.

이에 따라 상기한 바와 같은 젓가락 기술을 통해 난자의 핵을 제거하는 단계는 매우 정밀하고 세밀한 집중력을 요구하므로 이를 수행함에 있어 어려움을 겪는다는데 그 단점이 있는 것이다.Accordingly, the step of removing the nucleus of the egg through the chopstick technology as described above requires a very precise and detailed concentration, and thus has difficulty in performing this.

본 발명은 전기한 바와 같은 문제점을 극복하고자 안출된 것으로서, 블럭몸체의 일측에 난자의 외형과 일치하는 개방형 또는 밀폐형 요홈부를 형성하고, 블럭몸체의 타측에는 상기 요홈부와 관통되는 마이크로 니들 삽입공을 형성함으로서,The present invention has been made to overcome the problems as described above, and on one side of the block body to form an open or closed groove in accordance with the appearance of the egg, the other side of the block body micro needle insertion hole penetrating the groove By forming

난자의 핵 제거 및 이식을 하고자 하는 경우 상기 요홈부에 의해 난자가 요동하지 않게 되므로 구멍의 형성 및 핵 제거 과정이 보다 빠르고 안정적으로 이루어짐은 물론 그에 따른 불량률을 최소화하여 연구의 효율성 및 성과를 향상시키는 특징을 갖는 난자의 핵 제거용 작업블럭을 제공함에 본 발명의 목적이 있는 것이다.When the egg is to be removed and transplanted, the groove is not shaken by the groove so that the hole formation and nucleation process are faster and more stable, and the defect rate is minimized, thereby improving the efficiency and performance of the research. It is an object of the present invention to provide a working block for nucleus removal of an egg having characteristics.

이하, 본 발명의 바람직한 실시예를 설명한다.Hereinafter, preferred embodiments of the present invention will be described.

도 1은 본 발명에 따른 난자의 핵 제거용 작업블럭의 전체사시도이고, 도 2는 본 발명에 따른 난자의 핵 제거용 작업블럭의 또다른 실시예를 보인 사시도이며, 도 3은 본 발명에 따른 난자의 핵 제거용 작업블럭을 이용한 핵 제거 과정을 보인 평단면도이다.1 is an overall perspective view of the nucleus removal operation block of the egg according to the present invention, Figure 2 is a perspective view showing another embodiment of the nucleus removal operation block of the egg according to the present invention, Figure 3 according to the present invention This is a cross-sectional view showing the nucleation process using the egg removal task block.

블럭몸체(1)의 일측면에는 반구형의 요홈부(2)를 형성하고, 상기 요홈부(2)가 형성된 블럭몸체(1)의 타측면에는 상기 요홈부(2)와 관통되는 삽입공(3)을 형성하여,On one side of the block body (1) is formed a hemispherical groove (2), and the other side of the block body (1) in which the groove portion 2 is formed insertion hole (3) and the penetration hole (3) ),

상기 요홈부(2)에 의해 안착된 난자(10)를 마이크로 피펫(11)에 의해 취부 고정되게 하고, 별도의 마이크로 니들(12)을 상기 삽입공(3)으로 삽입하여 난자(10)의 핵을 제거할 수 있게 구성한 것이다.The oocyte 10 seated by the recess 2 is fixed by the micro pipette 11, and a separate micro needle 12 is inserted into the insertion hole 3 to nucleate the oocyte 10. It is configured to be removed.

이때, 상기 요홈부(2)는 반구형의 밀폐형 단부 또는 상,하부가 개방된 형태의 개방형 단부로 각각 구성될 수 있으며,At this time, the groove portion 2 may be composed of a hemispherical closed end or an open end of the upper, lower open form, respectively,

상기한 밀폐형 또는 개방형 단부를 갖는 단일의 요홈부(2)와 삽입공(3)을 갖는 단수형 작업블럭이나 복수개의 요홈부(2)와 삽입공(3)을 갖는 복수형 작업블럭으로 각각 구성할 수 있는 것이다.It can be composed of a single work block having a single concave portion 2 and the insertion hole (3) having a closed or open end or a plurality of work blocks having a plurality of grooves (2) and the insertion hole (3). It is.

또한, 상기한 복수형 작업블럭에 있어서, 이들 요홈부(2)의 크기가 점진적으로 확장되는 형태로도 구성할 수 있는 것이다.In addition, in the plurality of work blocks described above, the size of these recesses 2 can be configured to be gradually expanded.

이상과 같은 구성에 의한 본 발명 난자의 핵 제거용 작업블럭의 작용을 첨부도면에 의해 보다 상세히 설명하면 다음과 같다.Referring to the operation of the nucleus removal operation block of the egg of the present invention by the above configuration in more detail by the accompanying drawings as follows.

도시와 같이 본 고안 작업블럭은, 블럭몸체(1)의 일측면에 난자(10)가 삽입 안치될 수 있는 단수 혹은 복수의 요홈부(2)가 형성되어 있고, 그 타측면에는 상기 요홈부(2)와 관통 형성되는 삽입공(3)이 형성되어 있는 것이다.As shown in the drawing, the work block of the present invention has a single or a plurality of recesses 2 into which an egg 10 can be inserted and placed on one side of the block body 1, and the recesses on the other side thereof. 2) and the insertion hole (3) to be formed is formed.

따라서, 상기한 요홈부(2)를 통해 구상의 난자(10)를 안치시킨 상태에서 그 난자를 마이크로 피펫(Micro pipet)을 이용하여 위치 고정시킨 상태에서 마이크로 니들(Micro needle)을 상기 삽입공(3)으로 밀어넣어 이에 의해 상기한 난자(10)에 구멍을 형성하면 되는 것이다.Therefore, in the state where the spherical egg 10 is placed through the groove portion 2, the micro needle is inserted into the insertion hole in a state where the egg is fixed by using a micro pipet. 3) to form a hole in the egg 10 described above.

이와 같이 구멍이 형성되면 상기한 피펫을 이용하여 난자를 눌러 내부의 핵을 제거하면 되는 것이다.When the hole is formed as described above, the inner core can be removed by pressing the egg using the pipette.

또한, 상기한 요홈부(2)는 도 4a의 도시와 같이 상,하부가 개방된 형태의 요홈부(2)로 제작하거나 도 4b의 도시와 같이 반구형의 밀폐된 요홈부(2)로도 제작할 수 있는 것이다.In addition, the groove portion 2 may be manufactured as a groove portion 2 having an open top and bottom portions as shown in FIG. 4A or as a semi-spherical sealed groove portion 2 as shown in FIG. 4B. It is.

또한, 도 5a 및 도 5b의 도시와 같이 상기한 요홈부(2)를 복수개 형성하여 그 요홈부(2)의 형상을 개방형 또는 밀폐형으로 제작함으로서 동시에 여러개의 난자 연구를 수행할 수도 있는 것이다.In addition, as shown in FIGS. 5A and 5B, a plurality of the above-described recesses 2 may be formed, and the shape of the recesses 2 may be open or closed, so that several egg studies may be simultaneously performed.

특히, 난자(10)의 크기가 상이함에 착안하여 도 6a 및 도 6b의 도시와 같이 상기한 복수개의 요홈부(2)를 갖는 블럭몸체(1)에 있어 그 복수개의 요홈부(2)의 크기를 각기 상이하게 형성하되, 일측으로부터 타측으로 점차 확대되거나 축소되는 형태의 요홈부(2)를 갖도록 제작할 수도 있는 것이다.In particular, the size of the plurality of grooves 2 in the block body (1) having a plurality of grooves 2 described above, as shown in Figs. 6a and 6b in view of the different size of the egg 10 Each of them may be formed differently, but may be manufactured to have a recess 2 having a form gradually expanding or contracting from one side to the other side.

이와 같은 반구형의 밀폐 또는 개방된 요홈부(2)를 갖는 본 발명 작업블럭은 난자의 핵 제거시 매우 유용하게 사용되는 것으로서, 그 요홈부(2)에 의해 안정적으로 안치된 난자(10)는 유동하거나 미끄러져 이탈되지 아니하므로 매우 안정적인 실험 및 연구의 수행이 가능하게 되는 것으로서, 상기한 작업블럭은 현미경의 테이블이나 트레이에 직접 고정 설치하여 사용할 수도 있고, 별도의 클램프나 와이어 등의 고정수단을 사용하여 고정 설치한 후 사용함으로서 더욱 안정적인 작업을 꾀할 수 있게 된다.The work block of the present invention having such a semi-spherical closed or open recess 2 is very useful for nucleus removal of the egg, and the egg 10 stably settled by the recess 2 is flowed. It is possible to perform a very stable experiment and research because it does not slip or slip off, the above work block can be used by directly fixed to the table or tray of the microscope, or use a separate clamp or wire fixing means By using it after fixed installation, more stable work can be achieved.

이상과 같은 본 발명 난자의 핵 제거용 작업블럭은, 난자의 핵 제거 및 이식을 하고자 하는 경우 상기 요홈부에 의해 난자가 요동하지 않게 되므로 구멍의 형성 및 핵 제거 과정이 보다 빠르고 안정적으로 이루어짐은 물론 그에 따른 불량률을 최소화하여 연구의 효율성 및 성과를 향상시키는 효과가 있는 것이다.As described above, the operation block for removing the nucleus of the egg of the present invention does not oscillate by the recess when the nucleus of the egg is to be removed and transplanted. By minimizing the defect rate accordingly, it is effective to improve the efficiency and performance of the research.

Claims (6)

블럭몸체(1)의 일측면에는 반구형의 요홈부(2)를 형성하고, 상기 요홈부(2)가 형성된 블럭몸체(1)의 타측면에는 상기 요홈부(2)와 관통되는 삽입공(3)을 형성하여,On one side of the block body (1) is formed a hemispherical groove (2), and the other side of the block body (1) in which the groove portion 2 is formed insertion hole (3) and the penetration hole (3) ), 상기 요홈부(2)에 의해 안착된 난자(10)를 마이크로 피펫(11)에 의해 취부 고정되게 하고, 별도의 마이크로 니들(12)을 상기 삽입공(3)으로 삽입하여 난자(10)의 핵을 제거할 수 있게 구성함을 특징으로 하는 난자의 핵 제거용 작업블럭.The oocyte 10 seated by the recess 2 is fixed by the micro pipette 11, and a separate micro needle 12 is inserted into the insertion hole 3 to nucleate the oocyte 10. Nuclear removal work block, characterized in that configured to be removed. 제 1항에 있어서,The method of claim 1, 요홈부(2)는, 반구상의 밀폐형으로 구성됨을 특징으로 하는 난자의 핵 제거용 작업블럭.The recess 2 has a hemispherical enclosed type block for nucleus removal of an egg. 제 1항에 있어서,The method of claim 1, 요홈부(2)는, 상하측으로 개방된 반구형으로 구성됨을 특징으로 하는 난자의 핵 제거용 작업블럭.Concave groove (2), the operation block for nucleus removal of the egg, characterized in that consisting of a hemispherical shape open to the upper and lower sides. 제 1항에 있어서,The method of claim 1, 블럭몸체(1)에 단일의 요홈부(2)와 단일의 삽입공(3)을 형성하여 단수 작업을 가능케 구성함을 특징으로 하는 난자의 핵 제거용 작업블럭.Nucleus removal operation block, characterized in that the single body portion (2) and a single insertion hole (3) formed in the block body (1) to enable the singular operation. 제 1항에 있어서,The method of claim 1, 블럭몸체(1)에 복수개의 요홈부(2)와 복수개의 삽입공(3)을 연속 형성하여 복수 작업을 가능케 구성함을 특징으로 하는 난자의 핵 제거용 작업블럭.An operation block for nucleus removal of an ovum, characterized in that a plurality of grooves (2) and a plurality of insertion holes (3) are formed continuously in the block body (1) to enable a plurality of operations. 제 1항에 있어서,The method of claim 1, 블록몸체(1)에 복수개의 요홈부(2)와 복수개의 삽입공(3)을 형성하되, 상기 요홈부(2)는 점차 확장된 크기를 갖도록 구성함을 특징으로 하는 난자의 핵 제거용 작업블럭.A plurality of recesses 2 and a plurality of insertion holes 3 are formed in the block body 1, but the recesses 2 are nucleus removal operations, characterized in that configured to have a gradually expanded size. block.
KR1020060034811A 2006-04-18 2006-04-18 Working block for removal of a nucleus from an egg KR100712603B1 (en)

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Citations (10)

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JPH01112976A (en) * 1987-10-28 1989-05-01 Hitachi Ltd Micro-injection device
JPH06343478A (en) * 1993-06-08 1994-12-20 Hitachi Ltd Micro-injection method and apparatus
JPH08290377A (en) * 1995-04-21 1996-11-05 Prima Meat Packers Ltd Micro manipulation device and cell manipulating method using it
JPH11299496A (en) 1998-04-24 1999-11-02 Hitachi Ltd Vessel for oocyte and measuring device using the same
KR20010005423A (en) * 1999-06-30 2001-01-15 황우석 Method for enucleation of oocyte for producing a clone animal using somatic cell
JP2002065241A (en) 2000-09-01 2002-03-05 Hitachi Ltd Apparatus for introducing sample intoamphibian oocyte
WO2002081680A1 (en) 2001-04-05 2002-10-17 Institute Of Molecular Agrobiology Method for producing transgenic teleost fish
US20040235143A1 (en) 2003-05-21 2004-11-25 Fujitsu Limited System and apparatus for injecting substance into cell
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JP2005204624A (en) 2004-01-26 2005-08-04 Hitachi Ltd Needle for cell assay and high precision cell assay system

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01112976A (en) * 1987-10-28 1989-05-01 Hitachi Ltd Micro-injection device
JPH06343478A (en) * 1993-06-08 1994-12-20 Hitachi Ltd Micro-injection method and apparatus
JPH08290377A (en) * 1995-04-21 1996-11-05 Prima Meat Packers Ltd Micro manipulation device and cell manipulating method using it
JPH11299496A (en) 1998-04-24 1999-11-02 Hitachi Ltd Vessel for oocyte and measuring device using the same
KR20010005423A (en) * 1999-06-30 2001-01-15 황우석 Method for enucleation of oocyte for producing a clone animal using somatic cell
JP2002065241A (en) 2000-09-01 2002-03-05 Hitachi Ltd Apparatus for introducing sample intoamphibian oocyte
WO2002081680A1 (en) 2001-04-05 2002-10-17 Institute Of Molecular Agrobiology Method for producing transgenic teleost fish
US20040235143A1 (en) 2003-05-21 2004-11-25 Fujitsu Limited System and apparatus for injecting substance into cell
KR20050023483A (en) * 2003-08-28 2005-03-10 재단법인서울대학교산학협력재단 An improved method of intracytoplasmic sperm injection
JP2005204624A (en) 2004-01-26 2005-08-04 Hitachi Ltd Needle for cell assay and high precision cell assay system

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