WO2007119903A1 - Working block for removal of a nucleus from an egg - Google Patents
Working block for removal of a nucleus from an egg Download PDFInfo
- Publication number
- WO2007119903A1 WO2007119903A1 PCT/KR2006/002559 KR2006002559W WO2007119903A1 WO 2007119903 A1 WO2007119903 A1 WO 2007119903A1 KR 2006002559 W KR2006002559 W KR 2006002559W WO 2007119903 A1 WO2007119903 A1 WO 2007119903A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- egg
- nucleus
- holding portion
- removal
- working block
- Prior art date
Links
- 238000004891 communication Methods 0.000 claims description 2
- 235000013601 eggs Nutrition 0.000 abstract description 57
- 238000000034 method Methods 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 description 5
- 238000010367 cloning Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
Abstract
The present invention relates to a working block to be utilized in research of eggs in the field of biotechnology. In the working block for removal of a nucleus from an egg is formed at one side of the block body an open-type or closed-type holding portion that corresponds with the shape of egg, and on the other side a microneedle that communicates with the holding portion. Since the egg is not moved by the holding portion during the work of removing or transplanting a nucleus of the egg, not only the process of forming the hole and removing the nucleus can be carried out more quickly and stably, but also the resulting rejection rate is minimized to improve the performance and efficiency of research.
Description
Description
WORKING BLOCK FOR REMOVAL OF A NUCLEUS FROM
AN EGG
Technical Field
[1] The present invention relates to a working block to be used in egg research in the field of biotechnology, and more specifically to a working block for removal of a nucleus from an egg, by which, when removing a nucleus from an egg, the egg can be maintained stable and the microneedle is guided and inserted so that nucleus removal work can be conveniently carried out using micropipet.
[2]
Background Art
[3] Recently, various experiments are conducted using eggs in the field of biotechnology, and experiments of gene manipulation and cloning are being conducted through the method of removing the nucleus of the egg and transplanting a new nucleus. Such experiments are conducted for the purpose of pursuing health and happiness of mankind.
[4] As a widely used tool at the experiment stage of the biotechnology field using eggs like this, the micropipet is used. This micropipet is a small pipe used for measuring or moving a very small quantity of liquid, and in the case of removing the nucleus of an egg, it is also used as a tool to make the egg not move.
[5] In order to remove a nucleus from an egg by using such a micropipet, first a hole is punched in the egg using microneedle, and the egg is placed between the micropipette and microneedle. Next, these are pressed each other using micropipet as fixed board, and the nucleus of the egg is removed through the hole by the pressure. Such a method using so-called chopstick technique has been put to practical use.
[6] But because an egg is spherical and is very soft, it is very difficult to remove a nucleus from an egg using micropipet and microneedle. Namely, as described above, when the egg is pressed using micropipet and microneedle, the soft egg moves one way or another, so it is impossible to carry out nucleus removal work quickly and efficiently.
[7] Therefore, the stage of removing a nucleus from an egg through the chopstick technique requires very precise and elaborate concentration, so there are many difficulties in carrying out the work.
[8]
Disclosure of Invention Technical Problem
[9] Accordingly, it is an object of the present invention to provide a working block for removal of a nucleus from an egg, in which an open-type or a closed-type holding portion that corresponds with the shape of an egg is formed at one side of the block body, and a microneedle insert hole that communicates with the holding portion is formed at the other side of the block body, thereby the egg is not moved by the holding portion during the work of removing or transplanting a nucleus of the egg, so that not only the process of forming the hole and removing the nucleus is carried out more quickly and stably but also the resulting rejection rate is minimized so as to improve the performance and efficiency of research.
[10]
Technical Solution
[11] In accordance with the present invention, there is provided a working block for removal of a nucleus from an egg comprising: a semi- spherical holding portion 2 formed at one end portion of a block body 1, and an insert hole 3 which is inwardly extended from the one end portion that has said semi-spherical holding portion 2 formed thereon to the other end portion of the block body 1 in communication with said holding portion 2, and characterized by constructing in such a way that the egg 10 maintained by said holding portion 2 is fixed by means of micropipette 11, and another microneedle 12 is inserted into said insert hole 3 to remove a nucleus from the egg 10.
[12] Here, said holding portion 2 may have a closed-type semi-spherical cross-sectional structure or an open-type cross-sectional structure with the upper and lower portions open.
[13] Also, it is possible to construct a single-type working block that has a single holding portion 2 and a single insert hole 3 formed in the block body 1, or a multiple- type working block that has a plurality of holding portions 2 and a plurality of insert holes 3 formed successively in the block body 1 to make plural work possible.
[14] In addition, it is also possible to construct a multiple-type working block in such a manner that a plurality of holding portions 2 and a plurality of insert holes are formed in the block body 1, and the diameters of said holding portions 2 are gradually increased from one side pipet to the other side pipet.
Advantageous Effects
[15] Because a working block for removal of a nucleus from an egg according to the present invention can make the egg not move by means of the holding portion during the work of removing or transplanting a nucleus of the egg, not only the process of punching a hole and nucleus removal is more quickly and stably carried out, but also the resulting rejection rate is minimized so as to improve the performance and efficiency of research.
[16]
Brief Description of the Drawings
[17] These and other features, aspects, and advantages of preferred embodiments of the present invention will be more fully described in the following detailed description, taken accompanying drawings. In the drawings:
[18] Fig. 1 a perspective view showing a working block for removal of a nucleus from an egg according to one embodiment of the present invention;
[19] Fig. 2 is a perspective showing a working block for removal of a nucleus from an egg according to another embodiment of the present invention;
[20] Fig. 3 is a sectional view showing the process of removing a nucleus by using the working block for removal of a nucleus from an egg according to the present invention;
[21] Figs. 4 and 5 sectional views showing the end portion of the working block for removal of a nucleus from an egg according to the present invention, wherein Fig. 4 is a sectional view of the main part of a single working block having an open-type holding portion, and Fig. 5 is a sectional view of the main part of a single working block having a closed- type holding portion;
[22] Figs. 6 and 7 are sectional views showing a working block for removal of a nucleus from an egg according to another embodiment of the present invention, wherein Fig. 6 is a sectional view of the main part of a multiple working block having an open-type holding portion, and Fig. 7 is a sectional view of the main part of a multiple working block having a closed- type holding portion;
[23] Figs. 8 and 9 are sectional views of a working block for removal of a nucleus from an egg according to yet another embodiment of the present invention, wherein Fig. 8 is a sectional view of the main part of a multiple pipet with the diameter of the open-type holding portion gradually increased from one side pipet to the other side pipet, and Fig. 9 is a sectional view of a multiple pipet with the diameter of the semi-spherical shaped holding portion gradually increased from one side pipet to the other side pipet.
[24] * Description of numerals for main parts of the drawings *
[25] 1 : block body 2 : holding portion
[26] 3 : insert hole 10 : egg
[27] 11 : micropipette 12 : microneedle
[28]
Best Mode for Carrying Out the Invention
[29] Below will be described in detail a working block for removal of a nucleus from an egg according a preferred embodiment of the present invention with reference to the accompanying drawings.
[30] Fig. 1 a perspective view showing a working block for removal of a nucleus from an egg according to one embodiment of the present invention, Fig. 2 is a perspective showing a working block for removal of a nucleus from an egg according to another embodiment of the present invention, and Fig. 3 is a sectional view showing the process of removing a nucleus by using the working block for removal of a nucleus from an egg according to the present invention.
[31] As shown in the drawings, the working block according to the present invention has a single holding portion or a plurality of holding portions 2 formed at one side of the block body 1 for the egg to be inserted and maintained, and has an insert hole 3 formed so as to communicate with the holding portion 2.
[32] Accordingly, with the spherical egg 10 maintained in the holding portion 2, fix the egg immovable using the micropipette, and form a hole in the egg 10 by pushing the microneedle into the insert hole 3.
[33] After a hole is formed in the egg like above, use a pipet to press the egg to remove the nucleus inside.
[34] And, the holding portion 2 can be fabricated into an open-type (i.e. U shaped) holding portion 2 with the upper and lower portions open as shown in Fig. 4, or as shown in Fig. 5, it can be fabricated into a closed-type (i.e. cylindrical shaped) holding portion 2 with the whole circumference closed as shown in Fig. 5.
[35] Also, as shown in Figs. 6 and 7, a plurality of the holding portions are formed, and the shape of the holding portions 2 is fabricated in an open type or a closed type, so that it is possible to carry out research on eggs simultaneously.
[36] In particular, in view of differences in the size of egg 10, as shown in Figs. 8 and 9, in the block body 1 having a plurality of holding portions, the sizes of holding portions may be formed differently, and the pipet may as well be fabricated in such a manner that as you go from one side pipet to the other side pipet, for instance, as you go from down to up the diameter of the holding portion is gradually increased, or conversely decreased.
[37] The working block of the present invention having a semi-spherical closed or open- type holding portion 2 can be used very usefully when removing a nucleus from an egg. That is, because the egg 10 maintained stable by the holding portion 2 wouldn't float or slip to drift away, it is possible to carry out experiment or research very stably. Also, the aforementioned working block may as well use by fixing it directly to a table or tray of microscope, and by fixing it using a fixing means such as clamp or wire, work can be carried out more stably.
[38]
Industrial Applicability
[39] In the working block for removal of a nucleus from an egg, since the egg is not moved by the holding portion during the work of removing or transplanting a nucleus of the egg, not only the process of forming the hole and removing the nucleus can be carried out more quickly and stably, but also the resulting rejection rate is minimized to improve the performance and efficiency of research.
Claims
[1] A working block for removal of a nucleus from an egg comprising: a semi-spherical holding portion 2 formed at one end portion of a block body 1, and an insert hole 3 which is inwardly extended from the one end portion that has said semi-spherical holding portion 2 formed thereon to the other end portion of the block body 1 in communication with said holding portion 2, and characterized by constructing in such a way that the egg 10 maintained by said holding portion 2 is fixed by means of micropipette 11, and another microneedle 12 is inserted into said insert hole 3 to remove a nucleus from the egg 10.
[2] The working block for removal of a nucleus from an egg of claim 1, characterized in that said holding portion 2 has a semi- spherical closed-type cross- sectional structure.
[3] The working block for removal of a nucleus from an egg of claim 1, characterized in that said holding portion 2 has an open-type cross-sectional structure with the upper and lower portions open.
[4] The working block for removal of a nucleus from an egg of claim 1, characterized by constructing in such a way that a single holding portion 2 and a single insert hole 3 are formed in the block body 1 to make single work possible.
[5] The working block for removal of a nucleus from an egg of claim 1, characterized by constructing in such a way that a plurality of holding portions 2 and a plurality of insert holes 3 are successively formed in the block body 1 to make plural work possible.
[6] The working block for removal of a nucleus from an egg of claim 1, characterized by constructing in such a way that a plurality of holding portions 2 and a plurality of insert holes 3 are formed in the block body 1, and the diameters of said holding portions 2 are gradually increased from one side pipet to the other side pipet.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2006-0034811 | 2006-04-18 | ||
| KR1020060034811A KR100712603B1 (en) | 2006-04-18 | 2006-04-18 | Working block for removal of a nucleus from an egg |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2007119903A1 true WO2007119903A1 (en) | 2007-10-25 |
Family
ID=38269225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/KR2006/002559 WO2007119903A1 (en) | 2006-04-18 | 2006-06-30 | Working block for removal of a nucleus from an egg |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR100712603B1 (en) |
| WO (1) | WO2007119903A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08290377A (en) * | 1995-04-21 | 1996-11-05 | Prima Meat Packers Ltd | Micro manipulation device and cell manipulating method using it |
| KR20010005423A (en) * | 1999-06-30 | 2001-01-15 | 황우석 | Method for enucleation of oocyte for producing a clone animal using somatic cell |
| KR20050023483A (en) * | 2003-08-28 | 2005-03-10 | 재단법인서울대학교산학협력재단 | An improved method of intracytoplasmic sperm injection |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2624719B2 (en) * | 1987-10-28 | 1997-06-25 | 株式会社日立製作所 | Micro injection device |
| JPH06343478A (en) * | 1993-06-08 | 1994-12-20 | Hitachi Ltd | Micro-injection method and apparatus |
| JPH11299496A (en) | 1998-04-24 | 1999-11-02 | Hitachi Ltd | Vessel for oocyte and measuring device using the same |
| JP3587774B2 (en) | 2000-09-01 | 2004-11-10 | 株式会社日立製作所 | Amphibian oocyte sample introduction device |
| WO2002081680A1 (en) | 2001-04-05 | 2002-10-17 | Institute Of Molecular Agrobiology | Method for producing transgenic teleost fish |
| JP2004344036A (en) | 2003-05-21 | 2004-12-09 | Fujitsu Ltd | Substance-transducing device and substance-transducing system |
| JP2005204624A (en) | 2004-01-26 | 2005-08-04 | Hitachi Ltd | Needle for cell assay and high precision cell assay system |
-
2006
- 2006-04-18 KR KR1020060034811A patent/KR100712603B1/en not_active IP Right Cessation
- 2006-06-30 WO PCT/KR2006/002559 patent/WO2007119903A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08290377A (en) * | 1995-04-21 | 1996-11-05 | Prima Meat Packers Ltd | Micro manipulation device and cell manipulating method using it |
| KR20010005423A (en) * | 1999-06-30 | 2001-01-15 | 황우석 | Method for enucleation of oocyte for producing a clone animal using somatic cell |
| KR20050023483A (en) * | 2003-08-28 | 2005-03-10 | 재단법인서울대학교산학협력재단 | An improved method of intracytoplasmic sperm injection |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100712603B1 (en) | 2007-05-02 |
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