WO2007119904A1 - Micro pipet for removal of a nucleus from an egg - Google Patents

Micro pipet for removal of a nucleus from an egg Download PDF

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Publication number
WO2007119904A1
WO2007119904A1 PCT/KR2006/002560 KR2006002560W WO2007119904A1 WO 2007119904 A1 WO2007119904 A1 WO 2007119904A1 KR 2006002560 W KR2006002560 W KR 2006002560W WO 2007119904 A1 WO2007119904 A1 WO 2007119904A1
Authority
WO
WIPO (PCT)
Prior art keywords
egg
micropipet
nucleus
pipet
semispherical
Prior art date
Application number
PCT/KR2006/002560
Other languages
French (fr)
Inventor
Byung Jin Kim
Original Assignee
Byung Jin Kim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Byung Jin Kim filed Critical Byung Jin Kim
Publication of WO2007119904A1 publication Critical patent/WO2007119904A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B81—MICROSTRUCTURAL TECHNOLOGY
    • B81B—MICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
    • B81B2201/00—Specific applications of microelectromechanical systems
    • B81B2201/05—Microfluidics
    • B81B2201/057—Micropipets, dropformers

Definitions

  • the present invention relates to a micropipet used in the field of biotechnology, and more specifically to a micropipet for removal of a nucleus from an egg, by which easier work is possible by stably fixing an egg through a holding slot formed at one end portion of the micropipet when removing or transplanting the nucleus of the egg.
  • micropipet As a widely used tool at the experiment stage of the biotechnology field using eggs like this, the micropipet is used.
  • This micropipet is a small pipe used for measuring or moving a very small quantity of liquid, and in the case of removing the nucleus of an egg, it is also used as a tool to make the egg not move.
  • a micropipet for removal of a nucleus from an egg comprising: an inlet hole 3 formed inside of said micropipet, and a semispherical holding portion 5 which is formed at an end portion of a glass pipe 4 composing the body of said micropipet in communication with said inlet hole 3 and maintains an egg 10 spherical during the work of removing a nucleus from the egg.
  • This micropipet can be made in various other shapes as well. Namely, besides a single micropipet, the present invention is characterized by the possibility of being applied to various forms of micropipet in public domain.
  • the semispherical holding portion 5 can have a closed-type cross-sectional structure or an open-type cross-sectional structure with the upper and lower portions open.
  • the spherical egg 10 is maintained by the holding portion 5 with various shapes.
  • the micropipet for removal of a nucleus from an egg according to the present invention can improve the performance and efficiency of research, because not only faster and stable work is possible but also the resulting rejection rate is minimized, since the egg is not moved during the work of removing or transplanting the nucleus from an egg.
  • FIG. 1 is a perspective view showing an end portion of a micropipet according to one embodiment of the present invention
  • FIG. 2 is a perspective view showing an end portion of the a micropipet according to an other embodiment of the present invention.
  • FIGs. 3 and 4 are sectional views of a micropipet according to the present invention constructed in a form of a single pipet, wherein Fig. 3 is a sectional view of the main portion of a single pipet having a semispherical open-type holding portion, and Fig. 4 is a sectional view of the main portion of single pipet having a semispherical closed- type holding portion;
  • FIGs.5 and 6 are sectional views of a micropipet according to the present invention constructed in a form of a multiple-type pipet, wherein Fig. 5 is a sectional view of the main portion of a multiple-type pipet having semispherical open-type holding portions, and Fig. 6 is a sectional view of the main portion of a multiple-type pipet having semispherical closed- type holding portions; and
  • Figs. 7 and 8 are sectional views of a micropipet according to the present invention constructed in a form of a multiple-type pipet, wherein Fig. 7 is a sectional view of the main portion of a multiple-type pipet with the diameter of the semispherical open-type holding portion gradually increasing as you go from one side to the other side, and Fig. 8 is a sectional view of a multiple-type pipet with the diameter of the semispherical closed-type holding portion gradually increasing as you go from one side to the other side.
  • FIG. 1 is a perspective view showing an end portion of a micropipet according to an embodiment of the present invention
  • Fig. 2 is a perspective view showing an end portion of a micropipet according to another embodiment of the present invention.
  • the micropipet of the present invention in which the shape of one end portion is improved in various kinds of micropipet generally used.
  • the construction of a micropipet according to one embodiment of the present invention comprises a glass pipe 4 having an inlet hole inside and composing the body of the pipet, a holding portion 5 formed at one end portion of the glass pipe 4, and a button (not shown) communicating with the inlet hole 3 inside of the glass pipe.
  • a button (not shown) communicating with the inlet hole 3 inside of the glass pipe.
  • various shapes of micropipet such as a lever type or a rotating control ring type have been developed and used.
  • one side of the egg 10 is pushed by using a holding portion 5 formed at one end portion of the glass pipe 4 composing the micropipet. In this state, a hole is punched in the egg using microneedle from the other side of the egg to remove the nucleus of the egg.
  • a holding portion 5 having such a size that the egg 10 can be placed properly is formed at one end portion of the glass pipe 4.
  • the holding portion 5 can be made to have the top and bottom open and have a semispherical open-type cross-section, or as shown in Fig. 4, it can be made also into a semispherical closed- type holding portion 5.
  • the holding portion of the glass pipe 4 can be constructed into a multiple-type pipet. Namely, by forming a semi- spherical open-type or semi-spherical closed-type holding portion 5 at the end portion of the glass pipe 4 having a plurality of inlet holes 3 so as to communicate with one end of these inlet holes 3, it is possible to experiment and treat a plurality of eggs 10 using multiple-type pipet.
  • the size of the holding portion 5 is formed differently each other.
  • the pipet can be fabricated in such a shape that as you go from one side to the other side, for instance, as you go toward the upper side higher than the diameter of the holding portion located on the lower side, the diameter of the holding portion is gradually increased or conversely reduced.
  • the micropipet of the present invention having a semispherical open-type or semispherical closed-type holding portion 5 like above can be used very usefully when removing a nucleus from the egg. Namely, since the egg 10 maintained immovable by the holding portion 5 does not float or drift away from the pipet end portion, a very stable experiment becomes possible.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a micropipet used in the field of biotechnology. In a micropipet for removing or transplanting a nucleus of an egg is formed at an end portion of the pipet an open or closed-type holding portion that corresponds with the shape of the egg, so that the egg can be fixed more stably during the nucleus removal work through the holding portion formed at the end portion of the pipet. Since the egg does not move during the work of removing or transplanting the nucleus of the egg, not only faster and stable work is possible, but also the resulting rejection rate can be minimized so as to improve the performance and efficiency of research.

Description

Description
MICROPIPET FOR REMOVAL OF A NUCLEUS FROM AN
EGG
Technical Field
[1] The present invention relates to a micropipet used in the field of biotechnology, and more specifically to a micropipet for removal of a nucleus from an egg, by which easier work is possible by stably fixing an egg through a holding slot formed at one end portion of the micropipet when removing or transplanting the nucleus of the egg.
[2]
Background Art
[3] Recently, various experiments are conducted using eggs in the field of biotechnology, and experiments of gene manipulation and cloning are being conducted through the method of removing the nucleus of the egg and transplanting a new nucleus. Such experiments are conducted for the purpose of pursuing health and happiness of mankind.
[4] As a widely used tool at the experiment stage of the biotechnology field using eggs like this, the micropipet is used. This micropipet is a small pipe used for measuring or moving a very small quantity of liquid, and in the case of removing the nucleus of an egg, it is also used as a tool to make the egg not move.
[5] But since an egg is spherical and very soft, it is very difficult to remove an egg using a micropipet like the one mentioned above. Namely, in case of making a hole in the egg for removing the nucleus by using microneedle, with one side of the egg supported with the end portion of the micropipet, the egg won't be fixed in one place but will move one way or another.
[6] Therefore, a very delicate and precise work is needed to punch a hole in the egg because of such a shape and property of the egg. Because of that, it takes not only a considerable time but also the egg bursts frequently in the process.
[7]
Disclosure of Invention
Technical Problem
[8] Accordingly, it is an object of the present invention to provide a micropipet for removal of a nucleus from an egg, in which an open or closed-type holding portion that corresponds with the shape of the egg is formed at one end portion of the micropipet so as to support the experimental egg more stably through the holding portion, so that, during the work of removing or transplanting the nucleus of the egg, the work can not only carried out more quickly and more stably because the egg does not move, but also the resulting rejection rate is minimized to improve the performance and efficiency of research. [9]
Technical Solution
[10] In accordance with the present invention, there is provided a micropipet for removal of a nucleus from an egg comprising: an inlet hole 3 formed inside of said micropipet, and a semispherical holding portion 5 which is formed at an end portion of a glass pipe 4 composing the body of said micropipet in communication with said inlet hole 3 and maintains an egg 10 spherical during the work of removing a nucleus from the egg.
[11] This micropipet can be made in various other shapes as well. Namely, besides a single micropipet, the present invention is characterized by the possibility of being applied to various forms of micropipet in public domain.
[12] Also, the semispherical holding portion 5 can have a closed-type cross-sectional structure or an open-type cross-sectional structure with the upper and lower portions open.
[13] Moreover, it is also possible to construct a multiple-type pipet in which are assembled a plurality of pipets having a semispherical open-type holding portion or a semispherical closed-type holding portion.
[14] Furthermore, in case of constructing a multiple-type pipet having a semispherical open-type holding portion or a semispherical closed- type holding portion 5, it is possible to make the diameter of the semispherical holding portions 5 gradually increasing from one side pipet to the other side pipet.
[15] Preferably, the spherical egg 10 is maintained by the holding portion 5 with various shapes.
[16]
Advantageous Effects
[17] The micropipet for removal of a nucleus from an egg according to the present invention can improve the performance and efficiency of research, because not only faster and stable work is possible but also the resulting rejection rate is minimized, since the egg is not moved during the work of removing or transplanting the nucleus from an egg.
[18]
Brief Description of the Drawings
[19] These and other features, aspects, and advantages of preferred embodiments of the present invention will be more fully described in the following detailed description, taken accompanying drawings. In the drawings: [20] Fig. 1 is a perspective view showing an end portion of a micropipet according to one embodiment of the present invention;
[21] Fig. 2 is a perspective view showing an end portion of the a micropipet according to an other embodiment of the present invention;
[22] Figs. 3 and 4 are sectional views of a micropipet according to the present invention constructed in a form of a single pipet, wherein Fig. 3 is a sectional view of the main portion of a single pipet having a semispherical open-type holding portion, and Fig. 4 is a sectional view of the main portion of single pipet having a semispherical closed- type holding portion;
[23] Figs.5 and 6 are sectional views of a micropipet according to the present invention constructed in a form of a multiple-type pipet, wherein Fig. 5 is a sectional view of the main portion of a multiple-type pipet having semispherical open-type holding portions, and Fig. 6 is a sectional view of the main portion of a multiple-type pipet having semispherical closed- type holding portions; and
[24] Figs. 7 and 8 are sectional views of a micropipet according to the present invention constructed in a form of a multiple-type pipet, wherein Fig. 7 is a sectional view of the main portion of a multiple-type pipet with the diameter of the semispherical open-type holding portion gradually increasing as you go from one side to the other side, and Fig. 8 is a sectional view of a multiple-type pipet with the diameter of the semispherical closed-type holding portion gradually increasing as you go from one side to the other side.
[25] * Description of numerals for the main portions of the drawing *
[26] 3 : inlet hole 4: glass pipe
[27] 5 : holding portion 10 : egg
[28]
Best Mode for Carrying Out the Invention
[29] Below will be described in detail a micropipet for removal of a nucleus from an egg according to a preferred embodiment of the present invention with reference to the accompanying drawings.
[30] Fig. 1 is a perspective view showing an end portion of a micropipet according to an embodiment of the present invention, and Fig. 2 is a perspective view showing an end portion of a micropipet according to another embodiment of the present invention.
[31] As shown in the drawings, the micropipet of the present invention, in which the shape of one end portion is improved in various kinds of micropipet generally used. The construction of a micropipet according to one embodiment of the present invention comprises a glass pipe 4 having an inlet hole inside and composing the body of the pipet, a holding portion 5 formed at one end portion of the glass pipe 4, and a button (not shown) communicating with the inlet hole 3 inside of the glass pipe. Besides the pipet like this button type, various shapes of micropipet such as a lever type or a rotating control ring type have been developed and used.
[32] To remove a nucleus of an egg by using such a micropipet, one side of the egg 10 is pushed by using a holding portion 5 formed at one end portion of the glass pipe 4 composing the micropipet. In this state, a hole is punched in the egg using microneedle from the other side of the egg to remove the nucleus of the egg.
[33] As described above, since the egg is very soft and spherical, it easily floats or drifts away while a hole is punched or a nucleus is removed. To avoid this, in accordance with the present invention, a holding portion 5 having such a size that the egg 10 can be placed properly is formed at one end portion of the glass pipe 4.
[34] At this time, as shown in Fig. 3, the holding portion 5 can be made to have the top and bottom open and have a semispherical open-type cross-section, or as shown in Fig. 4, it can be made also into a semispherical closed- type holding portion 5.
[35] Also, as shown in Fig. 5 and Fig. 6, the holding portion of the glass pipe 4 can be constructed into a multiple-type pipet. Namely, by forming a semi- spherical open-type or semi-spherical closed-type holding portion 5 at the end portion of the glass pipe 4 having a plurality of inlet holes 3 so as to communicate with one end of these inlet holes 3, it is possible to experiment and treat a plurality of eggs 10 using multiple-type pipet.
[36] Especially, by paying attention to the point that the size of eggs 10 are different, as shown in Fig. 7 and Fig. 8, the size of the holding portion 5 is formed differently each other. Namely, the pipet can be fabricated in such a shape that as you go from one side to the other side, for instance, as you go toward the upper side higher than the diameter of the holding portion located on the lower side, the diameter of the holding portion is gradually increased or conversely reduced.
[37] The micropipet of the present invention having a semispherical open-type or semispherical closed-type holding portion 5 like above can be used very usefully when removing a nucleus from the egg. Namely, since the egg 10 maintained immovable by the holding portion 5 does not float or drift away from the pipet end portion, a very stable experiment becomes possible.
[38]
Industrial Applicability
[39] Since the egg does not move during the work of removing or transplanting the nucleus of the egg, not only faster and stable work is possible, but also the resulting rejection rate can be minimized so as to improve the performance and efficiency of research. [40]

Claims

Claims
[1] A micropipet for removal of a nucleus from an egg comprising: an inlet hole 3 formed inside of said micropipet, and a semispherical holding portion 5 which is formed at an end portion of a glass pipe 4 composing the body of said micropipet in communication with said inlet hole 3 and maintains an egg 10 spherical during the work of removing a nucleus from the egg. [2] The micropipet for removal of a nucleus from an egg of claim 1, characterized in that said semispherical holding portion 5 formed at the end portion of the glass pipe 4 of the micropipet has a closed-type cross-sectional structure. [3] The micropipet for removal of a nucleus from an egg of claim 1, characterized in that said semispherical holding portion 5 formed at the end portion of the micropipet has an open-type cross-sectional structure with the upper and lower portions open. [4] The micropipet for removal of a nucleus from an egg of claim 1, characterized in that said glass pipe 4 having a semispherical holding portion 5 is applied to a single-type pipet made up of single pipet. [5] The micropipet for removal of a nucleus from an egg of claim 1, characterized in that said glass pipes 4 having semispherical holding portions 5 are applied to a multiple-type pipet made up of a plurality of pipets. [6] The micropipet for removal of a nucleus from an egg of claim 1, characterized in that said glass pipes 4 having semispherical holding portions 5 are applied to the multiple-type pipet, and the diameters of the semispherical holding portions 5 are gradually increased from one side pipet to the other side pipet.
PCT/KR2006/002560 2006-04-18 2006-06-30 Micro pipet for removal of a nucleus from an egg WO2007119904A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2006-0034810 2006-04-18
KR1020060034810A KR100712602B1 (en) 2006-04-18 2006-04-18 Micro pipet for removal of a nucleus from an egg

Publications (1)

Publication Number Publication Date
WO2007119904A1 true WO2007119904A1 (en) 2007-10-25

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757892A (en) * 2011-04-27 2012-10-31 山东大学附属生殖医院 Micro-needle and method for separating sample

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08290377A (en) * 1995-04-21 1996-11-05 Prima Meat Packers Ltd Micro manipulation device and cell manipulating method using it
KR20010005423A (en) * 1999-06-30 2001-01-15 황우석 Method for enucleation of oocyte for producing a clone animal using somatic cell
KR20050023483A (en) * 2003-08-28 2005-03-10 재단법인서울대학교산학협력재단 An improved method of intracytoplasmic sperm injection

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63222680A (en) 1987-03-11 1988-09-16 Shimadzu Corp Injection needle apparatus for micromanipulation
JPH11299496A (en) 1998-04-24 1999-11-02 Hitachi Ltd Vessel for oocyte and measuring device using the same
JP2004344036A (en) 2003-05-21 2004-12-09 Fujitsu Ltd Substance-transducing device and substance-transducing system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08290377A (en) * 1995-04-21 1996-11-05 Prima Meat Packers Ltd Micro manipulation device and cell manipulating method using it
KR20010005423A (en) * 1999-06-30 2001-01-15 황우석 Method for enucleation of oocyte for producing a clone animal using somatic cell
KR20050023483A (en) * 2003-08-28 2005-03-10 재단법인서울대학교산학협력재단 An improved method of intracytoplasmic sperm injection

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757892A (en) * 2011-04-27 2012-10-31 山东大学附属生殖医院 Micro-needle and method for separating sample

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