CN102757892A - Micro-needle and method for separating sample - Google Patents

Abstract

The invention relates to a micro-needle and a method for separating a sample using the micro-needle. The micro-needle comprises a tube body, a connection tube and a needle head, wherein one end of the needle head, which is far away from the connection tube, adopts a groove structure. Through the adoption of the micro-needle provided by the invention, the required sample can be obtained accurately and rapidly.

Description

The method of microneedle and sample separation

Technical field

The present invention relates to a kind of micrurgy instrument, more specifically, the method that relates to a kind of microneedle and utilize this microneedle sample separation.

Background technology

Obtaining that small amounts of cells and tissue block sample test is the measuring means of often using in clinical.Maybe must guarantee under the situation of its maximum activity in that sample size is extremely precious, obtain sample accurately, effectively and seem particularly important.Trophocyte's biopsy with blastaea is an example, and the trophocyte that acquisition at present is used for biopsy on a small quantity is mainly through the stretching of microinjection pin and the auxiliary acquisition of laser.Choose the cell mass of requirement, block and outwards stretch, make being connected to elongate and attenuating between the cell mass chosen and the sample, cut in elongation place with laser then with the microinjection pin, thus the cell mass of acquisition needs.Because the microinjection pin do not have cutting function, in drawing process, often make whole blastaea separate, and the intervention of laser make heat effect strengthen embryo's damage with zona pellucida, limited its popularization clinically to a certain extent.

Summary of the invention

The purpose of this invention is to provide a kind of microneedle, be used for accurately, obtain effectively a small amount of cell or tissue piece that closely links to each other.

Microneedle of the present invention comprises body, pipe connecting and syringe needle, and syringe needle has groove away from pipe connecting one end.

The shape of said groove can be a random shape.As preferred implementation, being shaped as along the direction of caving in of said groove narrows down gradually; More preferably, said groove is a pointed shape; The angle of wedge angle is 0-90 °, is preferably 15 °-45 °.

The groove depth of said groove is 1-999 μ m, is preferably 2-300 μ m.

The number of said groove is at least 1, is preferably 2.

When syringe needle has 2 or 2 with upper groove, form front fork structure between the groove.Said front fork structure can be an Any shape, like trapezoidal, square, sharp fork-shaped or the like.Preferably, said front fork structure is sharp fork-shaped.

As preferred implementation, microneedle of the present invention comprises body, pipe connecting and syringe needle, and syringe needle has two grooves away from pipe connecting one end, and groove is a pointed shape, and the angle of wedge angle is 15 °-45 °.Form two point fork structures between two grooves.

In one embodiment, the axis direction of said syringe needle is consistent with the axis direction of body and pipe connecting, in line.

In another embodiment, the axis direction of the axis direction of said syringe needle and body and pipe connecting has angle, and angle is 0＜α≤90 °, is preferably 30 °≤α≤45 °.

Preferably, the internal diameter of syringe needle is 1-999 μ m, preferably 5-30 μ m; External diameter is 2-999 μ m, preferably 6-35 μ m.

Preferably, the pipe connecting of microneedle is tapered along body to syringe needle direction.

As preferred implementation, the length of microneedle is 2-25cm, is preferably 3-18cm.The external diameter of body is 100-10000 μ m, is preferably 500-1000 μ m; Internal diameter is 99-9999 μ m, is preferably 499-999 μ m; The transverse shape of body can be an Any shape, like circle, ellipse, trilateral, square or the like.

As preferred implementation, the material of said microneedle can be glass, quartz, plastics, stainless steel, copper, gold or silver-colored.Body, pipe connecting and syringe needle can be formed by the identical material integral production, are for example drawn by glass in one step to form; Also can form by differing materials welding or bonding.

Samplings such as microneedle of the present invention can be determined to embryo, cell mass, the tissue in each period, tissue, capillary vessel; Be handled easily, can adopt fixed arm on the micrurgy appearance, hand-held fixed arm or by fixing this microneedle of operator.

Another object of the present invention provides a kind of method of utilizing microneedle sample separation of the present invention, and this method comprises:

(1) groove with two microneedles blocks the junction that needs isolating sample and other sample respectively;

(2) relatively and rubbing in opposite directions, sample separation with the groove of two microneedles.

Said sample can be the embryo, cell mass, tissue block, tissue, capillary vessel in each period etc.

The present invention is fixed on groove through the groove structure that utilizes two microneedles with sample, and two microneedles are simultaneously from the both direction fixed sample, and move toward one another is cut the sample junction when being fixed, fast, effectively.Simultaneously, the needle axis of microneedle of the present invention and body axis can have angle, and the user can make the microneedle with different angles according to actual needs, under different situations, to experimentize.

The usefulness of microneedle of the present invention is: 1, accurately obtain needed sample apace; 2, need not reduce damage by means of laser and other instrument to sample; 3, simple to operate, do not need special experience, be convenient to operation; 4, go for more eurypalynous sample separation, the sample that particularly closely links to each other.

Description of drawings

Fig. 1: the structural representation of a kind of microneedle of the present invention, the axis direction of its middle tube body, pipe connecting and syringe needle is in line;

Fig. 2: the structural representation of the another kind of microneedle of the present invention, wherein the axis direction of the axis direction of syringe needle and body and pipe connecting has angle;

Fig. 3: needle head structure synoptic diagram with a groove.

Embodiment

Combine accompanying drawing and embodiment explanation the present invention, but the present invention is not limited to this at present.

As shown in Figure 1, microneedle of the present invention comprises body 1, pipe connecting 2 and syringe needle 3, and pipe connecting 2 is tapered along body 1 to syringe needle 3 directions, and syringe needle 3 has groove 4 away from pipe connecting 2 one ends.The effect that it will be appreciated by those skilled in the art that groove 4 is to block behind the sample cutting sample through the move toward one another between two operation pins, thus sample separation, and so groove 4 can be random shape, like square, trapezoidal, pointed shape etc.In the practical application, the shape of groove is excellent to narrow down gradually along the direction of caving in like pointed shape, and the angle of wedge angle can be 0-90 °, can be 15 °-45 ° better.The groove depth of groove is as the criterion to block sample, can be 1-999 μ m, preferably 2-300 μ m.The quantity of groove also can be arbitrarily, like a groove 4 " (like Fig. 3), two grooves 4 or 4 ' (like Fig. 1 or Fig. 2), three grooves etc.In the practical application, two grooves are best.When syringe needle has two or more grooves, form front fork structure between the groove, this front fork structure can be an Any shape with the groove structure difference, like trapezoidal, square, sharp fork-shaped etc.; Be optimum wherein, conveniently pierce through sample and operate with sharp fork-shaped.The length of front fork is consistent with the degree of depth of groove.

As shown in Figure 1, the axis direction of the axis direction of microneedle syringe needle 3 and body 1 and pipe connecting 2 can be consistent, a straight line, this moment, microneedle was straight; As shown in Figure 2, the axis direction of the axis direction of microneedle syringe needle 3 ' and body 1 ' and pipe connecting 2 ' has angle, and promptly microneedle is located to bend at syringe needle 3 ', and the angle scope is 0＜α≤90 °, is 30 °≤α≤45 ° better.It will be appreciated by those skilled in the art that in actually operating straight operation pin can satisfy different needs with curved operation pin.

For practical application needs, the internal diameter of syringe needle is 1-999 μ m, can be 5-30 μ m better; External diameter is 2-999 μ m, can be 6-35 μ m better.

The length of microneedle can be any length that is suitable for using, and like 2-25cm, is 3-18cm better.The D outer diameter 1 of body, inner diameter d 1 can be any scopes that is suitable for using, and can be 100-10000 μ m like D outer diameter 1, are 500-1000 μ m better; Inner diameter d 1 is 99-9999 μ m, is 499-999 μ m better.The body transverse shape is not limit, and can be circle, ellipse, trilateral, square or the like.

The material of such microneedle can be glass, quartz, plastics, stainless steel, copper, gold or silver-colored.Body, pipe connecting and front fork can be formed by the identical material integral production, are for example drawn by glass in one step to form; Also can form by differing materials welding or bonding.

During with the microneedle sample separation; Needing with two microneedles fixed samples simultaneously, mainly is to utilize the groove at syringe needle place to block sample, then with the relatively also rubbing in opposite directions of groove of two microneedles; Utilize the effect cutting sample of groove, thereby reach isolating purpose.Such sample can be the embryo, cell mass, tissue block, tissue, capillary vessel in each period etc., particularly connects sample closely.With respect to existing sample separation method, adopt micrurgy of the present invention to separate and exempted the damage that sample is produced because of too the pullling of pair cell, calcination to sample.

Describe concrete structure, the making processes of microneedle of the present invention and utilize this microneedle to carry out the process of sample separation through a specific embodiment below.

The making of microneedle

As material, adopt this area routine techniques to draw kapillary with the kapillary of external diameter 0.1cm, internal diameter 0.08cm, to obtain length be 18cm, comprise the operation pin embryo of body, pipe connecting and syringe needle, and pipe connecting is tapered along the direction of body to syringe needle.The syringe needle position of sanding operation pin embryo forms two pointed shape grooves, and groove angle is 35 °, and groove depth is 35 μ m; The internal diameter of syringe needle is 30 μ m, and external diameter is 35 μ m.Form two point fork structures between two grooves, promptly obtain microneedle.

The use of microneedle

Embodiment 1:

Get the blastaea that a trophocyte is partly hatched.With two syringe needle external diameters is that 35 μ m, internal diameter are the junction that the groove of the microneedle of 30 μ m blocks trophocyte and zona pellucida; With the groove of two microneedles relatively and towards the relative direction rubbing; The trophocyte of zona pellucida outside separates with the inner trophocyte of zona pellucida.

Embodiment 2:

Get the blastaea that a trophocyte is hatched fully.With two syringe needle external diameters is that 30 μ m, internal diameter are the junctions that the groove of the microneedle of 25 μ m blocks trophocyte and zona pellucida; With the groove of two microneedles relatively and towards the relative direction rubbing; The trophocyte of zona pellucida outside separates with the inner trophocyte of zona pellucida.

Embodiment 3:

Get the part germinal layer structure of from embryoid body, separating.With two be 20 μ m to external diameter, internal diameter be the groove of the microneedle of 18 μ m block the root of that part of germinal layer structure that will obtain; With the groove of two forked operation pins relatively and towards the relative direction rubbing; The part germinal layer piece that will obtain separate with main body germinal layer piece.

Claims (10)

1. a microneedle comprises body, pipe connecting and syringe needle, it is characterized in that, syringe needle has groove away from pipe connecting one end.

2. microneedle according to claim 1 is characterized in that, being shaped as along the direction of caving in of said groove narrows down gradually, and the groove number is at least 1, and groove depth is 1-999 μ m, is preferably 2-300 μ m.

3. microneedle according to claim 2 is characterized in that, said groove is a pointed shape, and the angle of wedge angle is 0-90 °, is preferably 15 °-45 °.

4. microneedle according to claim 2 is characterized in that, when syringe needle has 2 or 2 with upper groove, forms front fork structure between the groove, trapezoidal, the square or sharp fork-shaped of being shaped as of said front fork structure.

5. according to any one described microneedle of claim 1-4, it is characterized in that the axis direction of said syringe needle is consistent with the axis direction of body and pipe connecting, in line; Perhaps the axis direction of the axis direction of said syringe needle and body and pipe connecting has angle, and angle is 0＜α≤90 °, is preferably 30 °≤α≤45 °.

6. according to any one described microneedle of claim 1-4, it is characterized in that the internal diameter of said syringe needle is 1-999 μ m, preferably 5-30 μ m; External diameter is 2-999 μ m, preferably 6-35 μ m.

7. according to any one described microneedle of claim 1-4, it is characterized in that the length of microneedle is 2-25cm, is preferably 3-18cm; The external diameter of body is 100-10000 μ m, is preferably 500-1000 μ m; Internal diameter is 99-9999 μ m, is preferably 499-999 μ m.

8. according to any one described microneedle of claim 1-4, it is characterized in that the material of said microneedle is glass, quartz, plastics, stainless steel, copper, gold or silver-colored.

9. method of utilizing any one described microneedle sample separation of claim 1-8 comprises:

(1) groove with two microneedles blocks the junction that needs isolating sample and other sample respectively;

(2) relatively and rubbing in opposite directions, sample separation with the groove of two microneedles.

10. method according to claim 9 is characterized in that, said sample is cell mass, tissue block, tissue, the capillary vessel in each period.

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