CN103865756B - A kind of support for the unicellular tubulature of PGD - Google Patents
A kind of support for the unicellular tubulature of PGD Download PDFInfo
- Publication number
- CN103865756B CN103865756B CN201410104884.5A CN201410104884A CN103865756B CN 103865756 B CN103865756 B CN 103865756B CN 201410104884 A CN201410104884 A CN 201410104884A CN 103865756 B CN103865756 B CN 103865756B
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- locking piece
- support
- guide rail
- clamping ring
- fixed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Life Sciences & Earth Sciences (AREA)
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- Genetics & Genomics (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention relates to a kind of support for the unicellular tubulature of PGD, comprise support and the adjustment assembly of skeleton construction, two sides that support is relative are respectively provided with one group of adjustment assembly, adjustment assembly comprises longitudinal direction and regulates guide rail, governor lever, lateral adjustments assembly, jig arm and locking piece, guide rail is longitudinally regulated to be fixed on the side of support, governor lever is sleeved on and longitudinally regulates on guide rail and fixed by locking piece, lateral adjustments assembly comprises clamping ring I and clamping ring II, clamping ring I to be sleeved on governor lever and to be fixed by locking piece, jig arm is passed clamping ring II and is fixed by locking piece, jig arm one end of two groups of adjustment assemblies is provided with PCR pipe retaining clip and micrurgy arm retaining clip respectively by locking piece.The present invention can avoid because operator accurately cannot capture blastomere, and causes the phenomenon of the loss of blastomere, have can move flexibly, simple to operate, low cost of manufacture, can the advantage such as sterilizing, operating restraint be large.
Description
Technical field
The present invention relates to a kind of support for the unicellular tubulature of PGD.
Background technology
Along with the development of auxiliary procreation technology (Assisted Reproductive Technology, ART), third generation ART technology and PGD technology (Preimplantation Genetic Diagnosis, PGD) are developed into.This technology succeeded in nineteen ninety, mainly for crowd and the lying-in woman advanced in years of familial inheritance medical history, a blastomere is removed in its fetal development early utilization embryo biopsy technology, after molecular Biological Detection, normal embryo will be moved into uterus, and the embryo of exception is discarded, and then improve overall Population Birth quality, improve the health of the people.Therefore, extensive patients is also increasing to the demand of this technology.
At present the blastomere after embryo biopsy is loaded pcr tube (Polymerase Chain Reaction, PCR) (hereinafter referred to as PCR pipe) without special dress PCR pipe support.In every-day medical work, the main personal experience of laboratory operation person and the lab assistant of relying on uses homemade mouth suction pipe.Mouth suction pipe is the binding apparatus that infusion plastic pipe adds self-control glass syringe needle.Each lab assistant mouth suction pipe used is all identical, without unified operation specification.Along with the increase of patient numbers, lab assistant cannot be satisfied the demand.Because the reasons such as the working hour is long, tired, aprosexia cause the Loss Rate filling PCR pipe to increase, cause undetected, bring loss to patient.
At present, the device that can be used in the unicellular tubulature of PGD is micromanipulation system, and this micromanipulation system is electric device, relies on gear transmission power to change the direction of micrurgy arm.This operating system is mainly used in body-cell neucleus transplanting (Somatic Cell nuclear transplantation, SCNT), intracytoplasmic single sperm injection (intracytoplasmic sperm injection, ICSI) and the relevant accurate operation such as embryo biopsy (embryo biopsy), be a kind of operating gear be fixed on large-scale inverted microscope.Shortcomings such as (about 3.0cm) that this operating system exists that structure is quite complicated, cost is high, miles of relative movement is little, and non-dismountable, cannot move.
Summary of the invention
The main technical problem to be solved in the present invention is: provide that a kind of structure is simple, operating restraint be large, low cost of manufacture, can move flexibly, simple to operate; Can avoid because operator accurately cannot capture blastomere, and cause the support for the unicellular tubulature of PGD of the loss of blastomere, to solve above-mentioned problems of the prior art.
The technical scheme solved the problems of the technologies described above is: a kind of support for the unicellular tubulature of PGD, comprise support and the adjustment assembly of skeleton construction, two sides that described support is relative are respectively provided with one group of adjustment assembly, described adjustment assembly comprises longitudinal direction and regulates guide rail, governor lever, lateral adjustments assembly, jig arm and locking piece, described longitudinal direction regulates guide rail to be fixed on the side of support, governor lever is sleeved on and longitudinally regulates on guide rail and fixed by locking piece, lateral adjustments assembly comprises clamping ring I and clamping ring II connected vertically with clamping ring I, clamping ring I to be sleeved on governor lever and to be fixed by locking piece, jig arm is passed clamping ring II and is fixed by locking piece, jig arm one end of two groups of described adjustment assemblies is provided with PCR pipe retaining clip and micrurgy arm retaining clip respectively by locking piece, PCR pipe retaining clip and micrurgy arm retaining clip are provided with locking piece.
Described governor lever two ends respectively have a through hole, and the diameter of through hole is greater than the diameter longitudinally regulating guide rail, longitudinally regulate guide rail through through hole.
The skeleton construction that described support is made up of 2 cross tubes, 2 upper vertical bars, 2 lower vertical bars and 4 montants, described longitudinal direction regulates guide rail to be fixed between upper vertical bar and lower vertical bar and parallel with montant.
Described locking piece is securing bolt.
Owing to adopting said structure, the present invention has following beneficial effect:
1., compared with carrying out the operation of tubulature with existing employing mouth suction pipe, the present invention can avoid because operator accurately cannot capture blastomere, and the phenomenon causing blastomere to be lost.Adopt the present invention can combine with various stereoscope and use, PCR pipe and micrurgy arm can be fixed on the position that needs under the microscope by the present invention, by coordinating with microscopical, can capture blastomere accurately.The present invention is adopted to carry out the unicellular tubulature operation of PGD, whole operating process all can operate under microscopical observation, operation accurately, operating process does not need the personal experience of too much dependence operator, therefore compared with carrying out the operation of tubulature with existing employing mouth suction pipe, the present invention can avoid accurately cannot capturing blastomere because operator lack experience, and causes the loss of blastomere.
2. can movement flexibly, operating restraint is large, and miles of relative movement of the present invention can reach more than 6cm.The present invention is a supporting structure, does not need to be fixed on certain position, can move to correct position as required.In addition, the present invention can regulate the fixed position of PCR pipe and micrurgy arm in top to bottom, left and right, front and rear, can also regulate the fixed angle of PCR pipe and micrurgy arm, flexible to operation.
3. simple to operate, without the need to special training, lab assistant all can operate.The present invention coordinates microscope to use, and a needs can operate microscope, does not need other special training, and lab assistant just can accurately complete the single celled tubulature operation of PGD.
4. low cost of manufacture, without accurate various electric devices, can sterilizing.Major parts of the present invention only has support, straight-bar and some locking pieces, and structure is simple, and manufacturing cost is compared with the existing micromanipulation system realizing accurate operation, and manufacturing cost reduces greatly.And the present invention does not have accurate various electric devices, can sterilizing.
Below, in conjunction with the accompanying drawings and embodiments the technical characteristic of a kind of support for the unicellular tubulature of PGD of the present invention is further described.
Accompanying drawing explanation
Fig. 1: one of a kind of support stereographic map for the unicellular tubulature of PGD of the present invention.
Fig. 2: a kind of support stereographic map two for the unicellular tubulature of PGD of the present invention.
Fig. 3: the lateral adjustments unit construction schematic diagram of the present invention.
Fig. 4: the governor lever structural representation of the present invention.
Fig. 5: it " recessed " shape governor lever structural representation of the present invention.
In figure: 1-support, 11-cross tube, 12-upper vertical bar, 13-lower vertical bar, 14-montant, 2-longitudinally regulates guide rail, 3-governor lever, 31-through hole, 32-securing bolt I, 4-lateral adjustments assembly, 41-clamping ring I, 42-clamping ring II, 43-securing bolt II, 44-securing bolt III, 5-jig arm, 51-securing bolt IV, 6-PCR pipe clip, 7-micrurgy arm retaining clip, 8-securing bolt V.
Embodiment
Embodiment 1: as shown in Figures 1 to 4, a kind of support for the unicellular tubulature of PGD, comprise support 1 and the adjustment assembly of skeleton construction, described support 1 is by 2 cross tubes 11, 2 upper vertical bars 12, the skeleton construction that 2 lower vertical bars 13 and 4 montants 14 form, two sides that described support 1 is relative are respectively provided with one group of adjustment assembly, described adjustment assembly comprises longitudinally adjustment guide rail 2, governor lever 3, lateral adjustments assembly 4, jig arm 5 and securing bolt, described longitudinal direction regulates guide rail 2 to be fixed on the side of support 1, namely guide rail 2 is longitudinally regulated to be fixed between upper vertical bar 12 and lower vertical bar 13 and parallel with montant 14, described governor lever 3 two ends respectively have a through hole 31, the diameter of through hole 31 is greater than the diameter longitudinally regulating guide rail 2, longitudinally regulate guide rail 2 through through hole 31, governor lever 3 can longitudinally regulate guide rail 2 to slide up and down, to realize the up-down adjustment to adjustment assembly, and fixed by securing bolt I 32, lateral adjustments assembly 4 comprises clamping ring I 41 and clamping ring II 42 connected vertically with clamping ring I 41, clamping ring I 41 to be sleeved on governor lever 3 and to be fixed by securing bolt II 43, jig arm 5 is also fixed by securing bolt III 44 through clamping ring II 42, jig arm 5 one end of two groups of described adjustment assemblies is provided with PCR pipe retaining clip 6 and micrurgy arm retaining clip 7 respectively by securing bolt IV 51, PCR pipe retaining clip 6 and micrurgy arm retaining clip 7 are provided with securing bolt V 8, thing is clamped for fixing, the clamping ring I 41 of lateral adjustments assembly 4 can to horizontally slip and around governor lever 3 axial rotation along governor lever 3, to realize regulating the right position of lateral adjustments assembly 4 and regulating the level angle of jig arm 5, jig arm 5 can be free to slide and rotate in clamping ring II 42, to realize position adjustments to PCR pipe retaining clip 6 and micrurgy arm retaining clip 7 and angular adjustment, PCR pipe retaining clip 6 and micrurgy arm retaining clip 7 can rotate around securing bolt IV 51, to realize the angular adjustment to PCR pipe retaining clip 6 or micrurgy arm retaining clip 7 and jig arm 5.
As shown in Figure 5, the one as the present embodiment converts, and also governor lever 3 two ends can be set to the groove of " recessed " shape structure, and groove is sleeved on and longitudinally regulates on guide rail 2.
Another kind as the present embodiment converts, described support can also adopt other structure formation, as long as can be convenient to mounting and adjusting assembly, described locking piece can also adopt other locking pieces except adopting securing bolt, as long as can be convenient to fixing and loosen associated components.
Use procedure of the present invention:
1. under support of the present invention being fixed on stereoscopic microscope.
2. the biopsy ware of blastomere after biopsy is housed is placed in the central authorities of Stereo microscope, adjusting focal length is the most clear to blastomere.
3. micrurgy arm is loaded onto operation pin, be installed on the right-hand member anchor of this support, adjustment syringe needle is extremely the most clear, tighten the screws.
4. blastomere and operation needle tip are in the same view plane of the same visual field.
5. rotating operation arm, draws blastomere to operating needle tip, mobile biopsy ware, blastomere and operation pin dislocation washing lotion is dripped (main component is that phosphate buffered saline buffer adds human serum albumin (PBS-HSA)), repeatedly cleans 3 times, change 3 washing lotions and drip.
6. be rotated further motion arm and draw the blastomere after cleaning to operating needle tip, raise motion arm, biopsy ware is shifted out visual field central authorities.
7. be fixed on pipe support by PCR pipe, move to stereoscopic microscope central authorities, adjusting focal length is to clear.
8. lower motion arm, operation pin syringe needle is placed at the bottom of PCR pipe, be sure not (preventing pollution operates pin and touches disconnected operation pin) at the bottom of contact pipe.
9. adjusting focal length is to the most clear, and rotation process arm, spue blastomere.
10. focusing is to high-visible blastomere, determines that blastomere has proceeded in PCR pipe.
11. raise motion arm, take out PCR pipe.Build PCR pipe lid and carry out mark.
12. repeat above-mentioned steps, fill next embryonic blastomeres.
Micrurgy arm of the present invention is identical with the micrurgy arm configuration used in micromanipulation system in prior art.
Claims (2)
1. the support for the unicellular tubulature of PGD, it is characterized in that: the support (1) and the adjustment assembly that comprise skeleton construction, two sides that described support (1) is relative are respectively provided with one group of adjustment assembly, described adjustment assembly comprises longitudinal direction and regulates guide rail (2), governor lever (3), lateral adjustments assembly (4), jig arm (5) and locking piece, described longitudinal direction regulates guide rail (2) to be fixed on the side of support (1), governor lever (3) is sleeved on and longitudinally regulates guide rail (2) upper and fixed by locking piece, lateral adjustments assembly (4) comprises clamping ring I (41) and clamping ring II (42) connected vertically with clamping ring I (41), clamping ring I (41) is sleeved on governor lever (3) and goes up and fixed by locking piece, jig arm (5) is through clamping ring II (42) and fixed by locking piece, jig arm (5) one end of two groups of described adjustment assemblies is provided with PCR pipe retaining clip (6) and micrurgy arm retaining clip (7) respectively by locking piece, PCR pipe retaining clip (6) and micrurgy arm retaining clip (7) are provided with locking piece, described governor lever (3) two ends respectively have a through hole (31), and the diameter of through hole (31) is greater than the diameter longitudinally regulating guide rail (2), longitudinally regulate guide rail (2) through through hole (31), the skeleton construction that described support (1) is made up of 2 cross tubes (11), 2 upper vertical bars (12), 2 lower vertical bars (13) and 4 montants (14), described longitudinal direction regulates guide rail (2) to be fixed between upper vertical bar (12) and lower vertical bar (13) and parallel with montant (14).
2. the support for the unicellular tubulature of PGD according to claim 1, is characterized in that: described locking piece is securing bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410104884.5A CN103865756B (en) | 2014-03-20 | 2014-03-20 | A kind of support for the unicellular tubulature of PGD |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410104884.5A CN103865756B (en) | 2014-03-20 | 2014-03-20 | A kind of support for the unicellular tubulature of PGD |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103865756A CN103865756A (en) | 2014-06-18 |
| CN103865756B true CN103865756B (en) | 2015-10-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410104884.5A Expired - Fee Related CN103865756B (en) | 2014-03-20 | 2014-03-20 | A kind of support for the unicellular tubulature of PGD |
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| Country | Link |
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| CN (1) | CN103865756B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861279B (en) * | 2016-05-16 | 2017-11-28 | 柳州市妇幼保健院 | A kind of automatic unicellular tubulature operating system for PGD |
| CN110628591A (en) * | 2019-08-21 | 2019-12-31 | 广州市妇女儿童医疗中心 | Embryo biopsy sample transfer method |
| CN112812953A (en) * | 2020-12-28 | 2021-05-18 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell filtering and separating method and system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4729646A (en) * | 1986-05-15 | 1988-03-08 | Bausch & Lomb Incorporated | Multi-use microscope having modular construction of extruded parts |
| CN101960998A (en) * | 2010-08-18 | 2011-02-02 | 常仲乐 | Multifunctional microscope operating system |
| CN102271646A (en) * | 2008-11-04 | 2011-12-07 | 斯蒂芬·马尔泽 | Fixture |
| CN203846014U (en) * | 2014-03-20 | 2014-09-24 | 柳州市妇幼保健院 | Bracket for implanting preimplantation genetic diagnosis single-cell tubing |
-
2014
- 2014-03-20 CN CN201410104884.5A patent/CN103865756B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4729646A (en) * | 1986-05-15 | 1988-03-08 | Bausch & Lomb Incorporated | Multi-use microscope having modular construction of extruded parts |
| CN102271646A (en) * | 2008-11-04 | 2011-12-07 | 斯蒂芬·马尔泽 | Fixture |
| CN101960998A (en) * | 2010-08-18 | 2011-02-02 | 常仲乐 | Multifunctional microscope operating system |
| CN203846014U (en) * | 2014-03-20 | 2014-09-24 | 柳州市妇幼保健院 | Bracket for implanting preimplantation genetic diagnosis single-cell tubing |
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| Publication number | Publication date |
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| CN103865756A (en) | 2014-06-18 |
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Granted publication date: 20151028 Termination date: 20170320 |