CN213085985U

CN213085985U - Anti-pollution nucleic acid extraction system

Info

Publication number: CN213085985U
Application number: CN202021468050.XU
Authority: CN
Inventors: 萧志挽
Original assignee: Xi'an Zhongke Zhipu Medical Laboratory Co ltd
Current assignee: Xi'an Zhongke Zhipu Medical Laboratory Co ltd
Priority date: 2020-07-23
Filing date: 2020-07-23
Publication date: 2021-04-30
Anticipated expiration: 2030-07-23

Abstract

The utility model discloses an anti-pollution nucleic acid extraction system, which comprises a stand and a conveying component; a stand: the side surface of the stand is movably connected with a protective cover, two ends of the surface of the stand are symmetrically provided with guide grooves, supports are connected in the guide grooves in a sliding mode, a carrying frame is arranged on the surface of the stand, a reaction liquid placing groove, a reaction tube placing groove and a sample placing groove are sequentially arranged in the carrying frame from left to right, and a moving assembly is arranged at the bottom of the stand; a conveying assembly: including the rack, the sliding frame, the apparatus further comprises a rotating shaft, a gear and a first motor, the top surface of support is located to the rack, sliding frame sliding connection is on the support, the top both sides of sliding frame are run through in the pivot, the gear locate in the pivot and with rack toothing, the side of sliding frame is located to first motor, and the output shaft of first motor and the one end fixed connection of pivot, be equipped with lifting unit on the conveying subassembly, this anti-pollution nucleic acid extraction system, can realize the automatic extraction of nucleic acid, and the work efficiency is improved, and effectively reduced contaminated probability, the practicality is stronger.

Description

Anti-pollution nucleic acid extraction system

Technical Field

The utility model relates to a medical equipment technical field specifically is an anti-pollution nucleic acid extraction system.

Background

Nucleic acid is a biopolymer, is an indispensable constituent substance of all known life forms, is a general name of deoxyribonucleic acid and ribonucleic acid, is the most important substance of all biomolecules, is widely present in all animal and plant cells and microorganisms, and in medical institutions, blood samples of patients need to be detected frequently, nucleic acid in the blood samples needs to be extracted, the existing extraction mode of nucleic acid in partial blood samples still depends on medical staff to perform manual extraction by using a pipetting gun, and when the number of samples is large, the efficiency is low, and the manual extraction process is completely exposed in the air, so that the samples are easy to be polluted.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome current defect, provide an anti-pollution nucleic acid extraction system, can realize the automatic extraction of nucleic acid, improved work efficiency to effectively reduced contaminated probability, the practicality is stronger, can effectively solve the problem in the background art.

In order to achieve the above object, the utility model provides a following technical scheme: a contamination-resistant nucleic acid extraction system comprising a rack and a transport assembly;

a stand: the side surface of the stand is movably connected with a protective cover, two ends of the surface of the stand are symmetrically provided with guide grooves, a support is connected in the guide grooves in a sliding manner, the surface of the stand is provided with a carrying frame, a reaction liquid placing groove, a reaction tube placing groove and a sample placing groove are sequentially arranged in the carrying frame from left to right, and the bottom of the stand is provided with a moving assembly;

a conveying assembly: the conveying device comprises a rack, a sliding frame, a rotating shaft, a gear and a first motor, wherein the rack is arranged on the surface of the top of a support, the sliding frame is connected to the support in a sliding mode, the rotating shaft penetrates through two sides of the top of the sliding frame, the gear is arranged on the rotating shaft and meshed with the rack, the first motor is arranged on the side face of the sliding frame, an output shaft of the first motor is fixedly connected with one end of the rotating shaft, and a lifting assembly is arranged on a conveying assembly;

wherein: still include the PLC controller, the side of pallet is located to the PLC controller, and the input of PLC controller is connected with external power source's output electricity, and the output of PLC controller is connected with the input electricity of first motor.

Further, lifting unit includes frame plate, square pole, second motor and screw rod, the side of sliding frame is located to the frame plate, square pole runs through the bottom surface of frame plate, the bottom surface of sliding frame is located to the second motor, screw rod threaded connection is in the middle part threaded hole of square pole, and the output shaft fixed connection of the top of screw rod and second motor, and the bottom of square pole is equipped with electronic pipette, and the input of second motor is connected with the output electricity of PLC controller, and electronic pipette is connected with the two-way electricity of PLC controller, and the rotation of the usable second motor of lifting unit drives the screw rod and rotates, and the screw rod passes through the threaded connection with square pole, and then can realize the lift of square pole, and then can drive electronic pipette and go up and down.

Further, the movable assembly comprises a connecting plate, an extension plate, a lead screw and a third motor, the two ends of the connecting plate are connected with the bottoms of the two sides of the support respectively, the extension plate is symmetrically arranged on the bottom surface of the stand, the two ends of the lead screw are respectively rotatably connected to the extension plates on the two sides, the third motor is arranged on the side surface of the stand, an output shaft of the third motor is fixedly connected with one end of the lead screw, the input end of the third motor is electrically connected with the output end of the PLC, the movable assembly can rotate by the aid of the third motor to drive the lead screw to rotate, the support can be driven by the connecting plate to linearly move under the action of the guide groove, and accordingly the electric liquid-.

Further, still include rifle head standing groove, rifle head and rifle head collecting vat, the both sides that carry the frame are located to rifle head standing groove and rifle head collecting vat symmetry, the rifle head is arranged in the rifle head standing groove, and the rubber sleeve on rifle head top and the bottom interference fit of electronic pipetting gun, and the rifle head in the rifle head standing groove can cup joint the rifle head in the bottom of electronic pipetting gun through the bottom interference fit with electronic pipette, the change of the rifle head of being convenient for avoids mutual pollution between the sample.

Further, still include branch and frid, the side of rifle head collecting vat is located to branch, the top of branch is located to the frid, and the draw-in groove at frid middle part and the bottom clearance fit of electronic pipetting gun, the frid through with the clearance fit of electronic pipetting gun bottom, utilize the rising of electronic pipetting gun, take off the rifle head from the bottom of electronic pipetting gun under the limiting displacement of frid.

Compared with the prior art, the beneficial effects of the utility model are that: the pollution-proof nucleic acid extraction system has the following advantages:

1. the rotation of the usable first motor of conveying assembly of this anti-pollution nucleic acid extraction system drives the pivot and rotates, and then drives gear revolve, and the gear is through the meshing with the rack, and then can drive the sliding frame and slide, can realize finally that electronic pipetting gun transfers sample and reaction liquid.

2. This anti-pollution nucleic acid extraction system's the rotation of the usable second motor of lifting unit drives the screw rod and rotates, and the screw rod is through the threaded connection with square pole, and then can realize the lift of square pole, and then can drive electronic liquid-transfering gun and go up and down, accomplish the collection.

3. The rotation of the usable third motor of this anti-pollution nucleic acid extraction system's removal subassembly drives the lead screw and rotates, and then the straight-line motion of accessible connecting plate drive support under the effect of guide slot, and then realizes the horizontal migration of liquid-transfering gun, realizes taking of rifle head.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic view of the cross-sectional structure of the present invention.

In the figure: the device comprises a stand 1, a PLC 11, a protective cover 12, a guide groove 2, a bracket 21, a carrying frame 3, a reaction liquid placing groove 31, a reaction tube placing groove 32, a sample placing groove 33, a gun head placing groove 34, a gun head 35, a gun head collecting groove 36, a conveying assembly 4, a rack 41, a sliding frame 42, a rotating shaft 43, a gear 44, a first motor 45, a lifting assembly 5, a rack plate 51, a square rod 52, a second motor 53, a screw rod 54, a moving assembly 6, a connecting plate 61, a support plate 62, a screw rod 63, a third motor 64, an electric pipetting gun 7, a support rod 8 and a trough plate 81.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: a contamination-preventive nucleic acid extraction system includes a rack 1 and a transport unit 4;

a stand 1: the side surface of the frame is movably connected with a protective cover 12, two ends of the surface of the frame 1 are symmetrically provided with guide grooves 2, a support 21 is connected in the guide grooves 2 in a sliding manner, a carrying frame 3 is arranged on the surface of the frame 1, a reaction liquid placing groove 31, a reaction tube placing groove 32 and a sample placing groove 33 are sequentially arranged in the carrying frame 3 from left to right, a moving assembly 6 is arranged at the bottom of the frame 1, the moving assembly 6 comprises a connecting plate 61, support plates 62, a screw 63 and a third motor 64, two ends of the connecting plate 61 are respectively connected with the bottom ends of two sides of the support 21, the support plates 62 are symmetrically arranged on the bottom surface of the frame 1, two ends of the screw 63 are respectively connected on the support plates 62 on two sides in a rotating manner, the third motor 64 is arranged on the side surface of the frame 1, an output shaft of the third;

the conveying component 4: comprises a rack 41, a sliding frame 42, a rotating shaft 43, a gear 44 and a first motor 45, wherein the rack 41 is arranged on the top surface of the support 21, the sliding frame 42 is connected on the support 21 in a sliding way, the rotating shaft 43 penetrates through two sides of the top of the sliding frame 42, the gear 44 is arranged on the rotating shaft 43 and is meshed with the rack 41, the first motor 45 is arranged on the side surface of the sliding frame 42, the output shaft of the first motor 45 is fixedly connected with one end of the rotating shaft 43, a lifting assembly 5 is arranged on the conveying assembly 4, the lifting assembly 5 comprises a frame plate 51, a square rod 52, a second motor 53 and a screw 54, the frame plate 51 is arranged on the side surface of the sliding frame 42, the square rod 52 penetrates through the bottom surface of the frame plate 51, the second motor 53 is arranged on the bottom surface of the sliding frame 42, the screw 54 is connected in a threaded hole in the middle part of the square rod 52, the top end of the screw 54 is fixedly connected with the output shaft of the second motor 53, the electric pipette 7 is in bidirectional electric connection with the PLC 11;

wherein: still include PLC controller 11, PLC controller 11 locates the side of stand 1, and PLC controller 11's input is connected with external power source's output electricity, and PLC controller 11's output is connected with first motor 45's input electricity.

Wherein: the automatic pipette tip taking device further comprises a tip placing groove 34, a tip 35 and a tip collecting groove 36, wherein the tip placing groove 34 and the tip collecting groove 36 are symmetrically arranged on two sides of the carrying frame 3, the tip 35 is arranged in the tip placing groove 34, and a rubber sleeve at the top end of the tip 35 is in interference fit with the bottom end of the electric pipette 7; still include branch 8 and frid 81, the side of rifle head collecting vat 36 is located to branch 8, and the top of branch 8 is located to frid 81, and the draw-in groove in frid 81 middle part and the bottom clearance fit of electronic pipetting gun 7.

The conveying assembly 4 can drive the rotating shaft 43 to rotate by using the rotation of the first motor 45, and further drive the gear 44 to rotate, and the gear 44 can drive the sliding frame 42 to slide by meshing with the rack 41.

When in use:

firstly, the rotation of the first motor 45 drives the rotation shaft 43 to rotate, and then drives the gear 44 to rotate, the gear 44 is meshed with the rack 41, and then drives the sliding frame 42 to slide, the electric pipette 7 is moved to the upper part of the reaction liquid placing groove 31, the rotation of the second motor 53 drives the screw 54 to rotate, the screw 54 is connected with the square rod 52 through the thread, and then the square rod 52 can descend, and then the electric pipette 7 can descend, and the reaction liquid is collected, after the collection is completed, the collected reaction liquid is injected into the reaction tube in the reaction tube placing groove 32 by the conveying component 4 and the lifting component 5, the screw 63 is driven to rotate by the rotation of the third motor 64, and then the linear movement of the bracket 21 can be driven by the connecting plate 61 under the action of the guide groove 2, so that the bottom end of the electric pipette 7 slides into the groove plate 81, and then the electric pipette 7 moves upwards, make rifle head 35 drop to rifle head collecting vat 36 under the limiting displacement of frid 81 in, then utilize conveying component 4, lifting unit 5 and removal subassembly 6, cover new rifle head 35 in rifle head standing groove 34 in the bottom of electronic pipetting gun 7, accomplish the change of rifle head, then gather the blood sample in sample standing groove 33, pour into the reaction tube into, change rifle head 35 again after finishing pouring into, then take out the reaction tube, vibrate the mixing, can obtain the nucleic acid sample that waits to detect.

It should be noted that the specific model of the core chip of the PLC controller 11 disclosed in this embodiment is siemens S7-400, the first motor 45, the second motor 53, the third motor 64, and the electric pipette 7 may be freely configured according to an actual application scenario, and the electric pipette 7 may be an E4 XLS + electric pipette available from mtjs ltd. The PLC controller controls the first motor 45, the second motor 53, the third motor 64 and the electric pipette 7 to work by adopting a method commonly used in the prior art.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A contamination-preventive nucleic acid extraction system characterized by: comprises a stand (1) and a conveying component (4);

stand (1): the side surface of the rack is movably connected with a protective cover (12), two ends of the surface of the rack (1) are symmetrically provided with guide grooves (2), the guide grooves (2) are connected with a support (21) in a sliding manner, the surface of the rack (1) is provided with a carrying frame (3), a reaction liquid placing groove (31), a reaction tube placing groove (32) and a sample placing groove (33) are sequentially arranged in the carrying frame (3) from left to right, and the bottom of the rack (1) is provided with a moving assembly (6);

transfer assembly (4): the automatic conveying device comprises a rack (41), a sliding frame (42), a rotating shaft (43), a gear (44) and a first motor (45), wherein the rack (41) is arranged on the top surface of a support (21), the sliding frame (42) is connected to the support (21) in a sliding mode, the rotating shaft (43) penetrates through two sides of the top of the sliding frame (42), the gear (44) is arranged on the rotating shaft (43) and meshed with the rack (41), the first motor (45) is arranged on the side face of the sliding frame (42), an output shaft of the first motor (45) is fixedly connected with one end of the rotating shaft (43), and a lifting assembly (5) is arranged on a conveying assembly (4);

wherein: still include PLC controller (11), the side of pallet (1) is located in PLC controller (11), and the input of PLC controller (11) is connected with external power source's output electricity, and the output of PLC controller (11) is connected with the input electricity of first motor (45).

2. The contamination-preventive nucleic acid extraction system according to claim 1, characterized in that: lifting unit (5) include frame plate (51), square pole (52), second motor (53) and screw rod (54), the side of sliding frame (42) is located in frame plate (51), square pole (52) run through the bottom surface of frame plate (51), the bottom surface of sliding frame (42) is located in second motor (53), screw rod (54) threaded connection is in the middle part threaded hole of square pole (52), and the top of screw rod (54) and the output shaft fixed connection of second motor (53), and the bottom of square pole (52) is equipped with electronic liquid-transfering gun (7), and the input of second motor (53) is connected with the output electricity of PLC controller (11), and electronic liquid-transfering gun (7) and PLC controller (11) two-way electricity is connected.

3. The contamination-preventive nucleic acid extraction system according to claim 1, characterized in that: remove subassembly (6) including connecting plate (61), extension board (62), lead screw (63) and third motor (64), the both ends of connecting plate (61) are connected with the bottom of support (21) both sides respectively, the bottom surface of pallet (1) is located to extension board (62) symmetry, the both ends of lead screw (63) are rotated respectively and are connected on extension board (62) of both sides, the side of pallet (1) is located in third motor (64), the output shaft of third motor (64) and the one end fixed connection of lead screw (63), and the input of third motor (64) is connected with the output electricity of PLC controller (11).

4. The contamination-preventive nucleic acid extraction system according to claim 1, characterized in that: the liquid transferring device is characterized by further comprising a gun head placing groove (34), a gun head (35) and a gun head collecting groove (36), wherein the gun head placing groove (34) and the gun head collecting groove (36) are symmetrically arranged on two sides of the carrying frame (3), the gun head (35) is arranged in the gun head placing groove (34), and a rubber sleeve at the top end of the gun head (35) is in interference fit with the bottom end of the electric liquid transferring gun (7).

5. The contamination-preventive nucleic acid extraction system according to claim 4, characterized in that: the liquid collection gun is characterized by further comprising a supporting rod (8) and a groove plate (81), wherein the supporting rod (8) is arranged on the side face of the gun head collection groove (36), the groove plate (81) is arranged on the top end of the supporting rod (8), and a clamping groove in the middle of the groove plate (81) is in clearance fit with the bottom end of the electric liquid transfer gun (7).