CN215162750U

CN215162750U - Electromagnetic method high flux nucleic acid extraction element

Info

Publication number: CN215162750U
Application number: CN202121287513.7U
Authority: CN
Inventors: 肖腾飞
Original assignee: Shanghai Xunbaihui Biotechnology Co ltd
Current assignee: Shanghai Xunbaihui Biotechnology Co ltd
Priority date: 2021-06-09
Filing date: 2021-06-09
Publication date: 2021-12-14
Anticipated expiration: 2031-06-09

Abstract

The utility model relates to the technical field of nucleic acid extraction, in particular to an electromagnetic high-flux nucleic acid extraction device, which comprises a base, the base is provided with a controller, the top of the base is also provided with at least one mounting groove, the bottom of the mounting groove is provided with a temperature control mechanism, the mounting groove is internally provided with a magnetic bar sleeve frame, a plurality of hollow magnetic rod sleeves with openings at the top ends are arranged on the magnetic rod sleeve frame, one side of the base is provided with a mounting seat, and the top end of the mounting seat is provided with a mounting plate, the top of the mounting plate is provided with a magnetic mechanism, the mounting seat is provided with a lifting mechanism for driving the mounting plate to lift up and down along the mounting seat, the magnetic mechanism comprises a plurality of magnetic rods and a power assembly used for driving the magnetic rods to rotate, and the base is further provided with a driving assembly used for driving the mounting seat to slide along the base. The electromagnetic high-flux nucleic acid extraction device is convenient for accelerating the adsorption process of magnetic beads, and improves the adsorption rate, so that the extraction efficiency is higher.

Description

Electromagnetic method high flux nucleic acid extraction element

Technical Field

The utility model relates to a nucleic acid extraction technical field especially relates to an electromagnetic method high flux nucleic acid extraction element.

Background

With the development of modern biotechnology and medicine, high-throughput nucleic acid extraction has become one of the important means of research in the biologically relevant field. The magnetic bead method is to lyse a cell tissue sample with lysis solution, wherein nucleic acid molecules dissociated from the sample are specifically adsorbed onto the surface of magnetic particles, and impurities such as protein are not adsorbed and remain in the solution. The magnetic beads are generally divided into three layers, including an inner layer of support structure, a middle magnetic layer, and a modification layer coated on the outermost side, which are generally negatively charged groups. The magnetic bead method for extracting nucleic acid is increasingly widely applied to the biological world and related subjects due to the advantages of high flux and automation.

The magnetic bead method automatic nucleic acid extractor is divided into a magnetic rod method and a suction method, and the magnetic rod method is used more due to higher efficiency. However, the existing magnetic rod method extraction device has low and incomplete adsorption efficiency on magnetic beads when in use, so that the magnetic beads are greatly lost after being transferred for many times, the extraction efficiency is low, and the extraction time is prolonged.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, an object of the utility model is to provide an electromagnetic method high flux nucleic acid extraction element, electromagnetic method high flux nucleic acid extraction element is convenient for accelerate the adsorption process of magnetic bead, and improves the adsorption efficiency for extraction efficiency is higher.

In order to achieve the technical effects, the utility model adopts the following technical scheme:

an electromagnetic high-flux nucleic acid extraction device comprises a base, wherein a controller is arranged on the base, the top of the base is also provided with at least one mounting groove, the bottom of the mounting groove is provided with a temperature control mechanism, the mounting groove is internally provided with a magnetic bar sleeve frame, a plurality of hollow magnetic rod sleeves with openings at the top ends are arranged on the magnetic rod sleeve frame, one side of the base is provided with a mounting seat, and the top end of the mounting seat is provided with a mounting plate, the top of the mounting plate is provided with a magnetic mechanism, the mounting seat is provided with a lifting mechanism for driving the mounting plate to lift up and down along the mounting seat, the magnetic mechanism comprises a plurality of magnetic rods and a power assembly for driving the magnetic rods to rotate, the base is also provided with a driving assembly for driving the mounting seat to slide along the base, the temperature control mechanism, the lifting mechanism, the magnetic mechanism and the driving mechanism are all electrically connected with the controller.

Further, the mounting base comprises a bottom plate and a vertical plate, the bottom plate is connected with the base in a sliding mode, the vertical plate is arranged above the bottom plate, the mounting plate is installed on the vertical plate in a sliding mode, the lifting mechanism comprises a ball screw shaft vertically arranged on the vertical plate, a screw motor used for driving the ball screw shaft to rotate is arranged in the bottom plate, a driving block is installed on the ball screw shaft in a rotating mode, and the mounting plate is fixedly connected with the driving block.

Further, actuating mechanism includes fixed mounting and is in linear electric motor of base one side, linear electric motor with the controller electricity is connected just linear electric motor is last to be equipped with the slider, slider and linear electric motor sliding connection just the top and the base fixed connection of slider.

Further, the bottom of mounting groove is equipped with first installation cavity, the mounting groove outside is equipped with the second installation cavity, temperature control mechanism is including locating a plurality of semiconductor refrigeration pieces in the first installation cavity with locate electric heating element in the second installation cavity, just the refrigeration face of semiconductor refrigeration piece is hugged closely the top surface setting of first installation cavity.

Furthermore, the first installation cavity is further provided with a heat dissipation plate below the semiconductor refrigeration piece, one side of the heat dissipation plate is tightly attached to the heat dissipation surface of the semiconductor refrigeration piece, a plurality of heat dissipation fins are arranged on one side, away from the semiconductor refrigeration piece, of the heat dissipation plate, and the base is provided with heat dissipation through holes communicated with the first installation cavity in corresponding positions of the heat dissipation fins.

Further, the magnetic force mechanism comprises a shell fixedly connected with the mounting plate, a connecting plate is horizontally arranged in the shell, a plurality of first bevel gears are uniformly arranged on the connecting plate at intervals, a driving shaft is arranged at the bottom of each first bevel gear, the bottom end of each driving shaft penetrates through the connecting plate and extends to the lower portion of the connecting plate, the magnetic rods are arranged below the connecting plate, the top ends of the magnetic rods are fixedly connected with the bottom end of the driving shaft, and the power assembly is arranged above the mounting plate and used for driving the first bevel gears to rotate synchronously.

Furthermore, the first bevel gears are arranged on the connecting plate in a straight line shape, and the arrangement direction is perpendicular to the moving direction of the mounting seat.

Furthermore, the power assembly comprises a rotating shaft horizontally arranged above the connecting plate, the rotating shaft is connected with the shell in a rotating mode, a plurality of second bevel gears are fixedly mounted on the rotating shaft and are in one-to-one correspondence with the first bevel gears in meshed mounting positions, and the power assembly further comprises a micro motor which is arranged inside the shell and used for driving the rotating shaft to rotate.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model provides a pair of electromagnetic high flux nucleic acid extraction element is used for driving the mount pad through actuating mechanism on the base and slides along the base, simultaneously, this magnetic force mechanism of cooperation elevating system drive goes up and down to can realize that the magnetic bead shifts in the bar magnet cover of difference, and at the transfer in-process, rotate the adsorption process in order to accelerate the magnetic bead through power component drive bar magnet, thereby improve adsorption efficiency, and reduce the loss of magnetic bead in the transfer. In addition, this electromagnetic method high flux nucleic acid extraction element is used for carrying out temperature control to the bar magnet cover still through setting up temperature control mechanism to carry out independent control to the temperature at the nucleic acid extraction in-process, in order to do benefit to and extract nucleic acid, guarantee its stable in structure.

Drawings

FIG. 1 is a schematic diagram of an overall cross-sectional structure of an electromagnetic high-throughput nucleic acid extraction device according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a partial enlarged structure at A of an electromagnetic high-throughput nucleic acid extraction device according to an embodiment of the present invention;

the reference signs are: 10, a base, 11, a controller, 20, a mounting groove, 21, a magnetic rod holder, 22, a magnetic rod sleeve, 30, a first mounting cavity, 31, a semiconductor cooling plate, 32, a heat dissipation plate, 321, a heat dissipation fin, 33, a heat dissipation fan, 40, a second mounting cavity, 41, an electric heating component, 50, a linear motor, 51, a slider, 61, a bottom plate, 62, a vertical plate, 621, a ball screw shaft, 622, a screw motor, 623, a driving block, 63, a mounting plate, 70, a housing, 71, a connecting plate, 72, a first bevel gear, 721, a driving shaft, 722, a magnetic rod, 73, a rotating shaft, 731, a second gear, 732, a second bevel gear, 74, a micro motor, 741, and a first gear.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

As shown in fig. 1-2, the electromagnetic high-throughput nucleic acid extraction device provided in this embodiment includes a base 10, a controller 11 is disposed on the base 10, at least one mounting groove 20 is further disposed on the top of the base 10, and a plurality of hollow magnetic rod sleeves 22 with top openings are detachably disposed in the magnetic rod sleeve frame 21. One side of base 10 is equipped with the mount pad, just the top of mount pad is equipped with mounting panel 63, the top of mounting panel 63 is equipped with magnetic mechanism, just be equipped with on the mount pad and be used for the drive mounting panel 63 is along the elevating system of mount pad oscilaltion, magnetic mechanism includes a plurality of bar magnets 722 and is used for the drive the rotatory power component of bar magnet 722, still be equipped with on the base 10 and be used for the drive the mount pad carries out gliding drive assembly along base 10, temperature control mechanism, elevating system, magnetic mechanism and actuating mechanism all with controller 11 electricity is connected. In specific implementation, the controller 11 controls the temperature control mechanism to return the temperature of the reagent in the magnetic rod sleeve 22 or keep the temperature at a low temperature, and the controller 11 controls the driving assembly to drive the mounting base to slide along the base 10 and simultaneously drives the mounting plate 63 to drive the magnetic force mechanism to move up and down in cooperation with the lifting mechanism, so that the magnetic beads are transferred in different magnetic rod sleeves 22. More specifically, during the transferring process, the magnetic rod 722 may be driven by the power assembly to rotate to accelerate the magnetic bead adsorption process, thereby improving the adsorption efficiency and reducing the loss of the magnetic beads during the transferring process.

In this embodiment, the mounting base includes a bottom plate 61 slidably connected to the base 10 and an upright plate 62 disposed above the bottom plate 61, the mounting plate 63 is slidably mounted on the upright plate 62, the lifting mechanism includes a ball screw shaft 621 vertically disposed on the upright plate 62, the ball screw shaft 621 is rotatably connected to the upright plate 62, a screw motor 622 for driving the ball screw shaft 621 to rotate is disposed in the bottom plate 61, a driving block 623 is rotatably mounted on the ball screw shaft 621, and the mounting plate 63 is fixedly connected to the driving block 623. The driving mechanism comprises a linear motor 50 fixedly installed on one side of the base 10, the linear motor 50 is electrically connected with the controller 11, a sliding block 51 is arranged on the linear motor 50, the sliding block 51 is connected with the linear motor 50 in a sliding mode, and the top end of the sliding block 51 is fixedly connected with the base 10. In practical implementation, the controller 11 controls the screw motor 622 to start to drive the ball screw shaft 621 to rotate forward or backward, so as to drive the driving block 623 to move up and down along the ball screw shaft 621 and drive the mounting plate to move up and down, thereby inserting the magnetic rod 722 into the magnetic rod sleeve 22 or taking the magnetic rod 722 out of the magnetic rod sleeve 22.

In this embodiment, the bottom of mounting groove 20 is equipped with first installation cavity 30, the mounting groove 20 outside is equipped with second installation cavity 40, temperature control mechanism is including locating a plurality of semiconductor refrigeration pieces 31 in the first installation cavity 30 with locate electric heating element 41 in the second installation cavity 40, electric heating element 41 can select electric heating wire. Specifically, the refrigerating surface of the semiconductor refrigerating sheet 31 is closely attached to the top surface of the first installation cavity 30. Meanwhile, in order to facilitate the heat dissipation of the semiconductor refrigeration piece 31, the first mounting cavity 30 is further provided with a heat dissipation plate 32 below the semiconductor refrigeration piece 31, one side of the heat dissipation plate 32 is tightly attached to the heat dissipation surface of the semiconductor refrigeration piece 31, one side of the heat dissipation plate 32, which is far away from the semiconductor refrigeration piece 31, is provided with a plurality of heat dissipation fins 321, and the base 10 is provided with heat dissipation through holes communicated with the first mounting cavity 30 at corresponding positions of the heat dissipation fins 321. Preferably, a heat dissipation fan 33 is further disposed between the heat dissipation through hole and the heat dissipation fins 321, and the heat dissipation fan 33 and the semiconductor refrigeration sheet 31 are electrically connected to the controller 11. In specific implementation, the controller 11 controls the semiconductor refrigeration sheet 31 or the electric heating assembly 41 to work alternatively, so as to preserve heat, heat or refrigerate the reagent in the installation groove 20. Correspondingly, each mounting groove 20 is internally provided with a temperature sensor which is electrically connected with the controller 11.

In this embodiment, the magnetic mechanism includes a housing 70 fixedly connected to the mounting plate 63, a connecting plate 71 is horizontally disposed in the housing 70, the connecting plate 71 is fixedly connected to the housing 70, a plurality of first bevel gears 72 are disposed on the connecting plate 71 at regular intervals, a driving shaft 721 is disposed at the bottom of the first bevel gears 72, the bottom end of the driving shaft 721 penetrates through the connecting plate 71 and extends to the lower side of the connecting plate 71, the driving shaft 721 and the first bevel gears 72 are both rotatably connected to the connecting plate 71, the magnetic rod 722 is disposed below the connecting plate 71, and the top end of the magnetic rod 722 is fixedly connected to the bottom end of the driving shaft 721. Specifically, the first bevel gears 72 are arranged in a line shape on the connecting plate 71, the arrangement direction is perpendicular to the moving direction of the mounting seat, and the power assembly is arranged above the mounting plate 63 and is used for driving the first bevel gears 72 to rotate synchronously. Specifically, the power assembly includes a rotating shaft 73 horizontally disposed above the connecting plate 71, the rotating shaft 73 is rotatably connected to the housing 70, a plurality of second bevel gears 732 are fixedly mounted on the rotating shaft 73, the second bevel gears 732 are engaged with the first bevel gears 72 and are in one-to-one correspondence, the power assembly further includes a micro motor 74 disposed inside the housing 70 and configured to drive the rotating shaft 73 to rotate, and the micro motor 74 is electrically connected to the controller 11. More specifically, the output end of the micro motor 74 is disposed above the connecting plate 71 and fixedly connected to the housing 70, the output end of the micro motor 74 is provided with a first gear 741, one end of the rotating shaft 73 is provided with a second gear 731, and the second gear 731 is meshed with the first gear 741. In specific implementation, the controller 11 controls the micro motor 74 to start to drive the first gear 741 to rotate so as to drive the second gear 731 to drive the rotating shaft 73 to rotate, the second bevel gear 732 rotates synchronously with the first bevel gear 72, and the second bevel gear 732 rotates with the first bevel gear 72, the driving shaft 721 and the magnetic rod 722 to stir the reagent containing magnetic beads, thereby improving the adsorption efficiency.

Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art will understand that the present invention can be modified or replaced with other embodiments without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims. The technology, shape and construction parts which are not described in detail in the present invention are all known technology.

Claims

1. An electromagnetic method high-flux nucleic acid extraction device is characterized in that: including base (10), be equipped with controller (11) on base (10), one mounting groove (20) has still been seted up at least at the top of base (10), mounting groove (20) bottom is equipped with temperature control mechanism, be equipped with bar magnet cover frame (21) in mounting groove (20), just be equipped with a plurality of top end openings and hollow bar magnet cover (22) on bar magnet cover frame (21), one side of base (10) is equipped with the mount pad, just the top of mount pad is equipped with mounting panel (63), the top of mounting panel (63) is equipped with magnetic force mechanism, just be equipped with on the mount pad and be used for driving elevating system that mount pad (63) goes up and down along the mount pad, magnetic force mechanism includes a plurality of bar magnets (722) and is used for driving the rotatory power component of bar magnet (722), still be equipped with on base (10) and be used for driving the mount pad carries out gliding drive assembly along base (10), the temperature control mechanism, the lifting mechanism, the magnetic mechanism and the driving mechanism are all electrically connected with the controller (11).

2. The electromagnetic high-throughput nucleic acid extraction device according to claim 1, wherein: the mounting seat comprises a bottom plate (61) connected with the base (10) in a sliding mode and a vertical plate (62) arranged above the bottom plate (61), the mounting plate (63) is mounted on the vertical plate (62) in a sliding mode, the lifting mechanism comprises a ball screw shaft (621) vertically arranged on the vertical plate (62), a screw motor (622) used for driving the ball screw shaft (621) to rotate is arranged in the bottom plate (61), a driving block (623) is mounted on the ball screw shaft (621) in a rotating mode, and the mounting plate (63) is fixedly connected with the driving block (623).

3. The electromagnetic high-throughput nucleic acid extraction device according to claim 1, wherein: the driving mechanism comprises a linear motor (50) fixedly installed on one side of the base (10), the linear motor (50) is electrically connected with the controller (11), a sliding block (51) is arranged on the linear motor (50), the sliding block (51) is connected with the linear motor (50) in a sliding mode, and the top end of the sliding block (51) is fixedly connected with the base (10).

4. The electromagnetic high-throughput nucleic acid extraction device according to claim 1, wherein: the bottom of mounting groove (20) is equipped with first installation cavity (30), the mounting groove (20) outside is equipped with second installation cavity (40), temperature control mechanism is including locating a plurality of semiconductor refrigeration pieces (31) in first installation cavity (30) with locate electric heating element (41) in second installation cavity (40), just the refrigeration face of semiconductor refrigeration piece (31) is hugged closely the top surface setting of first installation cavity (30).

5. The electromagnetic high-throughput nucleic acid isolation apparatus according to claim 4, wherein: first installation cavity (30) still are equipped with heating panel (32) in the below of semiconductor refrigeration piece (31), one side of heating panel (32) is hugged closely the cooling surface of semiconductor refrigeration piece (31), just heating panel (32) are kept away from one side of semiconductor refrigeration piece (31) is equipped with a plurality of heat radiation fins (321), just base (10) are in heat radiation fins (321) the corresponding position seted up with the radiating through hole of first installation cavity (30) intercommunication.

6. The electromagnetic high-throughput nucleic acid extraction device according to claim 1, wherein: the magnetic force mechanism comprises a shell (70) fixedly connected with the mounting plate (63), a connecting plate (71) is horizontally arranged in the shell (70), a plurality of first bevel gears (72) are uniformly arranged on the connecting plate (71) at intervals, a driving shaft (721) is arranged at the bottom of each first bevel gear (72), the bottom end of each driving shaft (721) penetrates through the connecting plate (71) and extends to the lower portion of the connecting plate (71), the magnetic rod (722) is arranged below the connecting plate (71), the top end of the magnetic rod (722) is fixedly connected with the bottom end of the driving shaft (721), and the power assembly is arranged above the mounting plate (63) and used for driving the first bevel gears (72) to synchronously rotate.

7. The electromagnetic high-throughput nucleic acid extraction device according to claim 6, wherein: the first bevel gears (72) are arranged on the connecting plate (71) in a straight line shape, and the arrangement direction is perpendicular to the moving direction of the mounting seat.

8. The electromagnetic high-throughput nucleic acid extraction device according to claim 6, wherein: the power assembly comprises a rotating shaft (73) horizontally arranged above the connecting plate (71), the rotating shaft (73) is rotatably connected with the shell (70), a plurality of second bevel gears (732) are fixedly mounted on the rotating shaft (73), the second bevel gears (732) are meshed with the first bevel gears (72) and are in one-to-one correspondence, and the power assembly further comprises a micro motor (74) which is arranged in the shell (70) and used for driving the rotating shaft (73) to rotate.