CN216073820U

CN216073820U - Amplification device for library establishment of high-throughput gene sequencing

Info

Publication number: CN216073820U
Application number: CN202121553922.7U
Authority: CN
Inventors: 肖腾飞
Original assignee: Shanghai Xunbaihui Biotechnology Co ltd
Current assignee: Shanghai Xunbaihui Biotechnology Co ltd
Priority date: 2021-07-09
Filing date: 2021-07-09
Publication date: 2022-03-18
Anticipated expiration: 2031-07-09

Abstract

The utility model relates to the technical field of gene sequencing instruments, in particular to an amplification device for library establishment of high-throughput gene sequencing, which comprises a shell, wherein the bottom of the shell is provided with four support legs, the top end of the shell is provided with an incubation groove, and a temperature control assembly for controlling the temperature of the incubation groove is arranged in the shell; a sample bracket is further arranged in the incubation groove, a sample fixing plate is arranged on the sample bracket, an upper cover is hinged to the top of the casing, a sample gland for compressing and sealing the sample fixing plate is arranged at the bottom of the upper cover, and a lifting assembly for driving the sample gland to move downwards is further arranged in the upper cover; the casing is internally provided with a controller, and the temperature control assembly and the lifting assembly are electrically connected with the controller. The amplification device for high-throughput gene sequencing library establishment can rapidly carry out temperature cycle on a sample, and meanwhile, can effectively avoid sample evaporation or pollution.

Description

Amplification device for library establishment of high-throughput gene sequencing

Technical Field

The utility model relates to the technical field of gene sequencing instruments, in particular to an amplification device for library establishment of high-throughput gene sequencing.

Background

The high throughput sequencing technology is also called as next generation sequencing technology, and the accuracy of gene sequence measurement is directly related to the judgment of people on life activities, such as cancer diagnosis, noninvasive prenatal diagnosis and the like. The method can simultaneously and parallelly carry out sequence determination on hundreds of thousands to millions of DNA molecules, so that the deep sequencing of the genome of one species is convenient and easy. Three temperature points of denaturation-annealing-extension are needed to be set in three steps based on the PCR principle in the process of establishing a gene sequencing library. In the standard reaction, a three-temperature-point method is adopted, namely, double-stranded DNA is denatured at 90-95 ℃, then is rapidly cooled to 40-60 ℃ for annealing, then is rapidly heated to 70-75 ℃ for extension, and a plurality of cycles are required to be repeatedly carried out. Therefore, frequent increase and decrease of the temperature of the reaction system are required. At present, for the establishment of a gene sequencing library, a gene sequencing instrument is generally required, but the currently used instrument has a poor temperature control effect, and has a slow temperature rise and temperature drop speed on reaction systems.

SUMMERY OF THE UTILITY MODEL

In order to solve the above technical problems, it is an object of the present invention to provide a library-structured amplification apparatus for high-throughput gene sequencing, which can rapidly perform temperature cycling on a sample and, at the same time, can effectively prevent evaporation or contamination of the sample.

In order to achieve the technical effect, the utility model adopts the following technical scheme:

an amplification device for library establishment of high-throughput gene sequencing comprises a machine shell, wherein the bottom of the machine shell is provided with four support legs, the top end of the machine shell is provided with an incubation groove, and a temperature control assembly for controlling the temperature of the incubation groove is arranged in the machine shell; a sample bracket is further arranged in the incubation groove, a sample fixing plate is arranged on the sample bracket, an upper cover is hinged to the top of the casing, a sample gland for compressing and sealing the sample fixing plate is arranged at the bottom of the upper cover, and a lifting assembly for driving the sample gland to move downwards is further arranged in the upper cover; the casing is internally provided with a controller, and the temperature control assembly and the lifting assembly are electrically connected with the controller.

Further, the temperature control component is including locating hatch first electric heating pipe of inslot, still be equipped with circulation tank in the casing, be equipped with second electric heating pipe in the circulation tank, circulation tank's bottom surface still fixed mounting has the semiconductor refrigeration piece, the refrigeration face of the refrigeration component of semiconductor with circulation tank closely laminates, circulation tank pass through the circulating line with hatch groove intercommunication, be equipped with the circulating pump on the circulating line, the circulating pump electricity is connected to the controller.

Furthermore, an amplification area is arranged in the middle of the sample fixing plate, a plurality of plug holes which are regularly distributed are arranged in the amplification area, the cross sections of the plug holes are in a step shape, sample tubes are correspondingly plugged in the plug holes, and the top ends of the sample tubes protrude out of the top end of the sample fixing plate.

Further, the sample gland includes annular curb plate, the top of annular curb plate with upper cover fixed connection, the inboard slidable mounting of annular curb plate has the clamp plate, the clamp plate with lifting unit's output fixed connection.

Furthermore, the lifting assembly comprises a first transmission shaft and a second transmission shaft which are arranged in the upper cover, the first transmission shaft and the second transmission shaft are parallel to each other, two ends of the first transmission shaft and two ends of the second transmission shaft are respectively provided with a pressing cam, the sample gland is further provided with a pressing rod below the pressing cam, the pressing rod is connected with the sample gland in a sliding manner, the bottom end of the pressing rod extends to the position below the upper cover and is fixedly connected with the pressing plate, and four groups of first reset springs for driving the pressing rod to move upwards are further arranged in the upper cover; the lifting assembly further comprises a driving motor arranged in the upper cover and used for driving the first transmission shaft and the second transmission shaft to rotate synchronously, and the output end of the driving motor is in transmission connection with the first transmission shaft and the second transmission shaft through a driving shaft.

Furthermore, the sample bracket is provided with an annular positioning groove, the positioning groove surrounds the periphery of the insertion hole, a flexible cushion layer is further arranged inside the positioning groove, and the bottom end of the annular side plate can be correspondingly embedded into the positioning groove.

Furthermore, a locking mechanism for positioning the annular side plate is further arranged on the sample bracket.

Further, the equal interval in both sides of annular curb plate is equipped with a plurality of mounting grooves uniformly, the equal slidable mounting in inboard of mounting groove has the location iron core, still be equipped with in the mounting groove and be used for the drive the inside second reset spring who contracts of location iron core, locking mechanism locates including corresponding a plurality of locking pore canals on the positioning groove lateral wall, still correspond on the sample bracket and be equipped with the electro-magnet.

Compared with the prior art, the utility model has the beneficial effects that: according to the amplification device for high-throughput gene sequencing library establishment, the incubation groove is arranged in the shell and used for controlling the temperature of the reaction system, meanwhile, the circulating water tank is arranged in the shell and used for preheating or precooling constant-temperature medium water, and the temperature in the incubation groove can be rapidly changed in the amplification process, so that the amplification efficiency is improved; in addition, the amplification device for high-throughput gene sequencing library establishment, provided by the utility model, is used for sealing the sample tube on the sample fixing plate by arranging the sample pressing cover at the bottom of the upper cover, so that the sample can be effectively prevented from being scattered and polluted, and the workload of manually sealing the sample is reduced.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a library-based amplification apparatus for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 2 is a top view of a library-building amplification apparatus for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 3 is a schematic cross-sectional view taken along line A-A of an amplification apparatus for library construction for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a partial enlarged structure at B of a library-based amplification apparatus for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of a partial enlarged structure at C of a library-based amplification apparatus for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 6 is a front view of a library-building amplification apparatus for high-throughput gene sequencing according to an embodiment of the present invention;

FIG. 7 is a schematic cross-sectional view at D-D of an amplification apparatus for library building of high-throughput gene sequencing according to an embodiment of the present invention;

reference numeral 10, a housing, 11, a support leg, 20, an incubation groove, 21, a first electric heating tube, 30, a circulating water tank, 31, a second electric heating tube, 32, a semiconductor refrigeration piece, 40, a sample bracket, 41, a positioning groove, 411, a flexible cushion layer, 42, a locking hole, 43, an electromagnet, 50, a sample fixing plate, 51, a sample tube, 60, an upper cover, 61, a driving motor, 62, a driving shaft, 621, a first bevel gear, 63, a first transmission shaft, 64, a second transmission shaft, 65, a pressing cam, 66, a second bevel gear, 70, an annular side plate, 71, a mounting groove, 711, a positioning iron core, 712, a second reset spring, 80, a pressing plate, 81, a flexible pressing pad, 82, a pressing rod, 821, a first reset spring, 90 and a controller.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

As shown in fig. 1 to 7, the amplification device for library establishment for high-throughput gene sequencing provided in this embodiment includes a housing 10, the bottom of the housing 10 is provided with four support legs 11, the top of the housing 10 is provided with an incubation groove 20, and a temperature control assembly for controlling temperature of the incubation groove 20 is disposed in the housing 10. Still be equipped with sample bracket 40 in hatching groove 20, sample bracket 40 with hatching groove 20 fixed connection, just be equipped with sample fixed plate 50 on the sample bracket 40. An amplification area is arranged in the middle of the sample fixing plate 50, a plurality of regularly arranged inserting holes are arranged in the amplification area, the cross sections of the inserting holes are in a step shape, sample tubes 51 are correspondingly inserted into the inserting holes, and the top ends of the sample tubes 51 protrude out of the top end of the sample fixing plate 50. The top of the casing 10 is hinged with an upper cover 60, a sample gland for compressing and sealing the sample fixing plate 50 is arranged at the bottom of the upper cover 60, and a lifting assembly for driving the sample gland to move downwards is further arranged in the upper cover 60. Specifically, the lifting assembly may control the sample gland to move downward, so that the bottom surface of the sample gland is closely attached to the top end of the sample tube 51, so as to seal the sample tube 51. A controller 90 is further arranged in the machine shell 10, and the temperature control assembly and the lifting assembly are electrically connected with the controller 90. In specific implementation, the temperature control assembly controls the temperature in the incubation groove 20, the sample tube 51 is directly inserted into the insertion hole, and the top surface of the sample tube 51 is sealed by the sample gland, so that evaporation, scattering and the like of a sample in a reaction process are avoided, and the water in the incubation groove 20 can be prevented from polluting the sample.

In this embodiment, the temperature control assembly includes a first electric heating pipe 21 disposed in the incubation groove 20, and the first electric heating pipe is used for heating the water in the incubation groove 20 at a constant temperature, so that the temperature of the incubation groove 20 is constant. Still be equipped with circulation tank 30 in the casing 10, circulation tank 30 is used for carrying on preheating or precooling to the water in the incubation groove 20 to improve incubation groove 20's cooling or rate of rise of temperature. Specifically, be equipped with second electric heating pipe 31 in the circulating water tank 30, the bottom surface of circulating water tank 30 still fixed mounting has semiconductor refrigeration piece 32, the refrigeration face of the refrigeration subassembly of semiconductor with circulating water tank 30 closely laminates, circulating water tank 30 pass through circulating line with incubation groove 20 intercommunication, be equipped with the circulating pump on the circulating line so that exchange the water in incubation groove 20 and the water tank, in order to control temperature in the incubation groove 20. Meanwhile, temperature sensors are arranged in the incubation tank 20 and the circulation water tank 30 so as to detect the water temperature, and the first electric heating pipe 21, the second electric heating pipe 31, the temperature sensors and the circulation pump are electrically connected to the controller 90.

In this embodiment, the sample gland includes an annular side plate 70, the top end of the annular side plate 70 is fixedly connected to the upper cover 60, a pressure plate 80 is slidably mounted on the inner side of the annular side plate 70, and the pressure plate 80 is fixedly connected to the output end of the lifting assembly. Specifically, the lifting assembly comprises a first transmission shaft 63 and a second transmission shaft 64 which are arranged inside the upper cover 60, the first transmission shaft 63 and the second transmission shaft 64 are parallel to each other, two ends of the first transmission shaft 63 and the second transmission shaft 64 are respectively provided with a pressing cam 65, the pressing cover is further provided with a pressing rod 82 below the pressing cam 65, the pressing rod 82 is connected with the pressing cover in a sliding manner, the bottom end of the pressing rod 82 extends to the lower part of the upper cover 60 and is fixedly connected with the pressing plate 80, and four groups of first return springs 821 for driving the pressing rod 82 to move upwards are further arranged inside the upper cover 60; specifically, the return spring is disposed inside the upper cover 60 and sleeved outside the pressure lever 82 to drive the pressure lever 82 to move upward. The lifting assembly further comprises a driving motor 61 disposed inside the upper cover 60 for driving the first transmission shaft 63 and the second transmission shaft 64 to rotate synchronously, and the driving motor 61 is electrically connected to the controller 90. The output end of the driving motor 61 is in transmission connection with the first transmission shaft 63 and the second transmission shaft 64 through a driving shaft 62. More specifically, two first bevel gears 621 are provided on the driving shaft 62, a second bevel gear 66 is provided on one end of each of the first transmission shaft 63 and the second transmission shaft 64, and the second bevel gear 66 is engaged with the first bevel gears 621. In specific implementation, the controller 90 controls the driving motor 61 to start, the driving motor 61 drives the driving shaft 62 to rotate, so that the driving shaft 62 drives the first transmission shaft 63 and the second transmission shaft 64 to rotate, so as to drive the pressing cam 65 to rotate, so as to drive the pressing rod 82 to press down, so as to drive the pressing cover to move downward until the pressing cover contacts with the upper surface of the sample tube 51, so as to achieve a sealing effect on the sample tube 51.

In this embodiment, the sample holder 40 is provided with an annular positioning groove 41, the positioning groove 41 surrounds the periphery of the insertion hole, a flexible cushion 411 is further disposed inside the positioning groove 41, and the bottom end of the annular side plate 70 can be correspondingly embedded into the positioning groove 41. So as to avoid the outward spilling of the sample and effectively protect the sample during the incubation process. Meanwhile, the bottom of the gland is also provided with a flexible pressure pad 81 to further seal and protect the sample tube 51.

In this embodiment, in order to further protect the sample, a locking mechanism for positioning the annular side plate 70 is further disposed on the sample holder 40. Specifically, a plurality of mounting grooves 71 are uniformly arranged on two sides of the annular side plate 70 at intervals, a positioning iron core 711 is slidably mounted on the inner side of each mounting groove 71, a second return spring 712 used for driving the positioning iron core 711 to contract inwards is further arranged in each mounting groove 71, the locking mechanism comprises a plurality of locking hole channels 42 correspondingly arranged on the side wall of the positioning groove 41, electromagnets 43 are correspondingly arranged on the sample bracket 40, and the electromagnets 43 are electrically connected to the controller 90. In practical implementation, the second return spring 712 can be extended under the attraction of the electromagnet 43 to drive the iron core to be correspondingly inserted into the locking hole 42, so that the connection between the annular side plate 70 and the sample holder 40 is performed and the locking state is always maintained during the incubation process of the sample, thereby further avoiding the contamination of the sample.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the utility model as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims

1. An amplification apparatus for library building for high-throughput gene sequencing, characterized by: the temperature control device comprises a machine shell (10), wherein four support legs (11) are arranged at the bottom of the machine shell (10), an incubation groove (20) is formed in the top end of the machine shell (10), and a temperature control assembly for controlling the temperature of the incubation groove (20) is arranged in the machine shell (10); a sample bracket (40) is further arranged in the incubation groove (20), a sample fixing plate (50) is arranged on the sample bracket (40), an upper cover (60) is hinged to the top of the casing (10), a sample gland for compressing and sealing the sample fixing plate (50) is arranged at the bottom of the upper cover (60), and a lifting assembly for driving the sample gland to move downwards is further arranged in the upper cover (60); still be equipped with controller (90) in casing (10), temperature control assembly and lifting unit all with controller (90) electricity is connected.

2. The apparatus for library-based amplification for high-throughput gene sequencing of claim 1, wherein: temperature control assembly is including locating first electric heating pipe (21) in hatching groove (20), still be equipped with circulation tank (30) in casing (10), be equipped with second electric heating pipe (31) in circulation tank (30), the bottom surface of circulation tank (30) still fixed mounting have semiconductor refrigeration piece (32), the refrigeration face of the refrigeration subassembly of semiconductor with circulation tank (30) closely laminates, circulation tank (30) through the circulating line with hatch groove (20) intercommunication, be equipped with the circulating pump on the circulating line, the circulating pump electricity is connected to controller (90).

3. The apparatus for library-based amplification for high-throughput gene sequencing of claim 1, wherein: an amplification area is arranged in the middle of the sample fixing plate (50), a plurality of plug holes which are regularly arranged are arranged in the amplification area, the cross sections of the plug holes are in a step shape, sample tubes (51) are correspondingly plugged in the plug holes, and the top ends of the sample tubes (51) protrude out of the top end of the sample fixing plate (50).

4. The apparatus of claim 3 for library building for high throughput gene sequencing, wherein: the sample gland includes annular curb plate (70), the top of annular curb plate (70) with upper cover (60) fixed connection, the inboard slidable mounting of annular curb plate (70) has clamp plate (80), clamp plate (80) with lifting unit's output fixed connection.

5. The apparatus of claim 4 for library building for high throughput gene sequencing, wherein: the lifting assembly comprises a first transmission shaft (63) and a second transmission shaft (64) which are arranged inside the upper cover (60), the first transmission shaft (63) and the second transmission shaft (64) are parallel to each other, two ends of the first transmission shaft (63) and the second transmission shaft (64) are respectively provided with a pressing cam (65), the sample pressing cover is further provided with a pressing rod (82) below the pressing cam (65), the pressing rod (82) is connected with the sample pressing cover in a sliding mode, the bottom end of the pressing rod (82) extends to the lower portion of the upper cover (60) and is fixedly connected with the pressing plate (80), and four groups of first return springs (821) for driving the pressing rod (82) to move upwards are further arranged inside the upper cover (60); the lifting assembly further comprises a driving motor (61) which is arranged inside the upper cover (60) and used for driving the first transmission shaft (63) and the second transmission shaft (64) to synchronously rotate, and the output end of the driving motor (61) is in transmission connection with the first transmission shaft (63) and the second transmission shaft (64) through a driving shaft (62).

6. The apparatus of claim 4 for library building for high throughput gene sequencing, wherein: the sample bracket (40) is provided with an annular positioning groove (41), the positioning groove (41) surrounds the periphery of the plug hole, a flexible cushion layer (411) is further arranged inside the positioning groove (41), and the bottom end of the annular side plate (70) can be correspondingly embedded into the positioning groove (41).

7. The apparatus of claim 6 for library building for high throughput gene sequencing, wherein: and a locking mechanism for positioning the annular side plate (70) is further arranged on the sample bracket (40).

8. The apparatus for library-based amplification for high-throughput gene sequencing of claim 7, wherein: the equal interval in both sides of annular curb plate (70) is equipped with a plurality of mounting grooves (71) uniformly, the equal slidable mounting in inboard of mounting groove (71) has location iron core (711), still be equipped with in mounting groove (71) and be used for the drive second reset spring (712) of location iron core (711) inside shrink, locking mechanism is including corresponding locating a plurality of locking pore (42) on positioning groove (41) lateral wall, still correspond on sample bracket (40) and be equipped with electro-magnet (43).