CN103878061B - A kind of full automatic magnetic separating device - Google Patents
A kind of full automatic magnetic separating device Download PDFInfo
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- CN103878061B CN103878061B CN201410105196.0A CN201410105196A CN103878061B CN 103878061 B CN103878061 B CN 103878061B CN 201410105196 A CN201410105196 A CN 201410105196A CN 103878061 B CN103878061 B CN 103878061B
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- movable stand
- blade
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- separating device
- microporous pipe
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Abstract
The invention discloses a kind of full automatic magnetic separating device, it comprises microporous pipe (101), bottom bracket, magnetic patch (204), movable stand (104), movable stand driver part, blade (201), blade upset driver part, blade fixed bar (302); Overturn driver part driven vane (201) around blade fixed bar (302) upset being fixed on movable stand (104) by blade, combine again and use the movable stand axial sliding moving frame of driver part (104), thereby the magnetic that can realize several functions state on single station (disperseing, manage end reunion, tube wall reunion etc.) separates, the present invention has higher magnetic-particle capture rate, prevented cross pollution, has frame for movement simple, small and exquisite flexibly and be easy to assembling and the advantage such as integrated.
Description
Technical field
The present invention relates to a kind of separator, the especially a kind of magnetic separating device that can realize the full-automatic multimode reunion of magnetic-particle, disperse.
Background technology
Biochemical separation is a requisite link during biological specimen is processed, traditional separation method is as phenol-chloroform method, Chelex100 method, salting out method etc., comprise that liquid-liquid separates, the step such as centrifugal, the required separator of these traditional separation methods is bulky, complex operation, length consuming time, is difficult to be integrated on self-reacting device, realize subsequent detection.
Compare above method, specific area that magnetic-particle has is large, superparamagnetism, be easy to the advantages such as finishing, is typically the most, and in the time applying external magnetic field, magnetic nanoparticle is magnetized, and is attracted on microporous pipe sidewall and realizes and reuniting; When magnetic field disappears, the magnetic on magnetic nanoparticle also disappears thereupon, and these particles will be dispersed in reactant liquor again, realize like this dispersion and the reunion of large biological molecule. At present, utilize magnetic nanoparticle to disperse and reunite easy and simple to handle efficiently, it apply more and more widely at biomedical sector, the prevalent means that has become that some routine biochemistries test, as nucleic acid extraction, gene sequencing, immunoassay etc.
Traditional magnetic separating device belongs to static-magnetic separator, and microporous pipe bracket and permanent magnet are generally provided, and such device only provides a kind of hold-down support that can make magnetic-particle enrichment. Cause like this in the time using such device to carry out experimental implementation, need between permanent magnet hold-down support and microporous pipe hold-down support, repeatedly carry out repeatedly artificial transfer microporous pipe work (for the appearing and subsiding of externally-applied magnetic field), this has brought a lot of inconvenience to experiment, has greatly increased the possibility of polluting simultaneously. And the device of this type only realized the reunion (singlet) of magnetic-particle in the single position of microporous pipe, the apparatus structure of disclosed similar patent is all very complicated at present, and inconvenience is integrated in the middle of the instrument of large instrument or miniaturization.
Therefore, along with the develop rapidly of Life Science Instruments, more realize magnetic-particle in the urgent need to the full automatic multi-function device of one and reunite flexibly and disperse, and can realize the controlled reunion at tubular container multiple location, this is also technical problem to be solved by this invention.
Summary of the invention
The deficiency existing in order to overcome prior art, the invention provides a kind of multi-functional automatic magnetic separating device, this device can be realized the various states of magnetic-particle in tubular container, comprising: magnetic-particle is reunited at container bottom, sidewall is reunited, or dispersion soln state etc.
Object of the present invention is achieved through the following technical solutions:
A kind of full-automatic magnetic separating device, comprises microporous pipe, bottom bracket, magnetic patch, also comprises movable stand, movable stand driver part, blade, blade upset driver part, blade fixed bar.
Described microporous pipe inserts in bottom bracket top; Described movable stand is placed in bottom bracket bottom; Described movable stand driver part comprises that movable stand drive motors, shaft coupling and movable stand drive leading screw; The output shaft of described movable stand drive motors drives leading screw to be connected by shaft coupling and movable stand, and movable stand drives the corresponding nut of leading screw to be arranged on movable stand.
One end of described blade is provided with magnetic patch; The other end of described blade is connected in blade fixed bar, and described blade fixed bar is fixedly installed on movable stand; Described blade upset driver part is connected in blade middle part; Described blade upset driver part is arranged on movable stand; Described blade upset driver part comprises blade connecting rod, kinematic link, blade turn drive motor; The output shaft clutch disk of described blade turn drive motor, one end of the distal end connection for transmission connecting rod of disk; The bottom of described blade connecting rod is connected on kinematic link; The other end of described blade connecting rod connects blade middle part.
As a further improvement on the present invention, described bottom bracket comprises microporous pipe bracket and main body base; Described microporous pipe inserts on microporous pipe bracket; Described microporous pipe bracket is fixed on main body base by bracket holddown groove.
As a further improvement on the present invention, described movable stand is embedded in bottom bracket by movable stand guide-track groove; Described movable stand guide-track groove is slip guiding device or rolling guide device. Movable stand drive motors can do moving axially with respect to microporous pipe by movable stand guide-track groove guiding movable stand.
As a further improvement on the present invention, between described movable stand and bottom bracket, be connected with buffer spring. The selecting of described buffer spring can guarantee that movable stand is moving back and forth the stability in process with respect to bottom bracket.
Described magnetic patch is permanent magnet or electromagnet; The material of described permanent magnet is selected from the one in Ru-Fe-Mn magnet, permanent ferrite, soft magnetic ferrite, SmCo magnetic material.
The material of each test tube plate of the present invention and bedplate has no particular limits, and can be prepared by general moulding process by resin, or be processed into by metallic plate, and magnetic separation device can integratedly be manufactured, and also can make by assembled.
Compared with prior art, technical scheme of the present invention has following beneficial effect:
(1) the present invention has overcome many deficiencies that existing magnetic separating device exists, realize the magnetic separating device of the several functions state (disperseing, manage end reunion, tube wall reunion etc.) of Full-automatic magnetic particle, make up technological gap, there is higher magnetic-particle capture rate, low cost of manufacture, difficulty of processing low, be easy to the advantages such as assembling.
(2) microporous pipe that the present invention contains magnetic-particle solution in the whole course of work is still on microporous pipe bracket all the time, having realized single station magnetic separates, so both save working space and made the effect that device is small and exquisite, ensured separation, can avoid again the interference between microporous pipe, greatly prevent cross pollution.
(3) by changing different microporous pipe brackets, thereby go for the microporous pipe of multiple volume, improved the versatility of magnetic separating device, realized " one multiplex ", reduced use cost.
(4) practical, this device is Open architecture, there is frame for movement simple, small and exquisite flexibly and be easy to the features such as realization, can be integrated on the devices such as large-scale biochemical analyzer, also can independently use as the magnetic separating device of miniaturization, such device will meet the actual demand realizing widely.
(5) separate by regulating microporous pipe bracket can control magnetic the position that rear magnetic nanoparticle is reunited on microporous pipe sidewall, thereby can effectively reduce the loss of magnetic-particle in automatized magnetic separation process, and then make magnetic separation system greatly reduced by the impact of mechanical displacement control accuracy.
Brief description of the drawings
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is internal structural map of the present invention.
Fig. 2 is the configuration state figure that magnetic-particle is dispersed in timer in solution.
Fig. 3 is that magnetic-particle is reunited at the configuration state figure of microporous pipe bottom timer.
Fig. 4 is that magnetic-particle is reunited at the configuration state figure of microporous pipe sidewall timer.
In figure: microporous pipe (101), microporous pipe bracket (102), bracket holddown groove (103), movable stand (104), movable stand guide-track groove (105), movable stand drive motors (106), movable stand drives leading screw (107), main body base (108), blade (201), blade connecting rod (202), disk (203), magnetic patch (204), buffer spring (205), kinematic link (206), blade turn drive motor (301), blade fixed bar (302).
Detailed description of the invention
Below with reference to accompanying drawing, preferred embodiment of the present invention is elaborated, so that clearer understanding objects, features and advantages of the present invention. It should be understood that embodiment shown in the drawings is not limitation of the scope of the invention, and just for the connotation of technical solution of the present invention is described. Hereinafter, the identical Reference numeral of identical with similar element numeral.
Referring to accompanying drawing 1, the described microporous pipe 101 in full-automatic magnetic separating device of the present invention inserts on microporous pipe bracket 102; Described microporous pipe bracket 102 is fixed on main body base 108 by bracket holddown groove 103. Described movable stand 104 is embedded on main body base 108 by movable stand guide-track groove 105; Described movable stand guide-track groove 105 is rolling guide device. Described movable stand driver part comprises that movable stand drive motors 106, shaft coupling and movable stand drive leading screw 107; The output shaft of described movable stand drive motors 106 drives leading screw 107 to be connected by shaft coupling and movable stand, and movable stand drives the corresponding nut of leading screw 107 to be arranged on movable stand 104.
Referring to accompanying drawing 2,3, the realization of blade turn over function of the present invention adopts the crank and rocker mechanism based on shutter, is the core of magnetic separating device. It is specifically configured to the blade fixed bar 302 of some framed bent at movable stand 104 tops, one end of every blade 201 connects blade fixed bar 302 using it as a fixed pivot, the other end of blade 201 is provided with and is suitable for magnetic-particle to be suspended in from them that solution wherein attracts and the magnetic patch 204 of certain gradient magnetic of separating, blade 201 middle parts are connected to blade connecting rod 202, and all parallel blade connecting rod 202 bottoms are articulated and connected on kinematic link 206; Blade turn drive motor 301 is arranged on movable stand 104 bottom surfaces, and blade turn drive motor 301 output shafts connect a disk 203, and the distal end of disk 203 is connected with one end of kinematic link 206; Between described movable stand 104 and main body base 108, be connected with buffer spring 205; Described magnetic patch 204 is permanent magnet; The material of described permanent magnet is Ru-Fe-Mn magnet.
The course of work of full-automatic magnetic separating device is described below:
(1) work is initial, the microporous pipe 101 filling with the magnetic-particle solution of material to be separated (as the biomolecule such as nucleic acid, protein or cell etc.) specific bond is placed on microporous pipe bracket 102, movable stand drive motors 106 shrinks and self-locking, buffer release spring 205; Blade turn drive motor 301 is in the initial position self-locking that powers on, disk 203 is connected fulcrum in lowermost end with kinematic link 206, the sagging magnetic patch 204 of realizing of blade 201 is away from microporous pipe 101 bottoms, this timer is in original state, as shown in Figure 2, the interior magnetic-particle solution of microporous pipe 101 is in dispersity.
(2) realizing magnetic-particle reunites in microporous pipe bottom, as shown in Figure 3, blade turn drive motor 301 rotates counterclockwise certain angle self-locking, kinematic link 206 is with moving vane connecting rod 202 to promote blade 201 and is up swung, blade 201 is in level, and magnetic patch 204 rests on microporous pipe 101 bottoms; The material to be separated that magnetic-particle in solution carries combination is adsorbed to bottom, reunites at the bottom of having realized pipe, and remaining liq can siphon away by modes such as pipettors, can realize target material and the separating of non-target substance.
(3) realize magnetic-particle and reunite at microporous pipe sidewall, as shown in Figure 4, movable stand drive motors 106 promotes movable stand 104 and moves axially certain distance along movable stand guide-track groove 105, compression buffer spring 205; Blade turn drive motor 301 rotates counterclockwise self-locking after certain angle, promotes pendulum on blade 201 and makes magnetic patch 204 rest in microporous pipe 101 sidewalls, and the target substance that the magnetic-particle in solution carries combination is adsorbed to sidewall, has realized sidewall reunion; Remaining liq can siphon away by modes such as pipettors, can realize target material and the separating of non-target substance.
The conversion of above-mentioned several duties can be realized by control system, in conjunction with liquid-transfering device, automatized magnetic separating experiment can be realized.
Last institute should be noted that; above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; wherein structure, the connected mode etc. of each parts all can change to some extent; other any do not deviate from equivalents, improvement, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (6)
1. a full-automatic magnetic separating device, comprises microporous pipe (101), bottom bracket, magnetic patch (204), characterized by further comprisingMovable stand (104), movable stand driver part, blade (201), blade upset driver part, blade fixed bar (302);
Described microporous pipe (101) inserts in bottom bracket top; Described movable stand (104) is placed in bottom bracket bottom; Described movable stand drivesDynamic component comprises that movable stand drive motors (106), shaft coupling and movable stand drive leading screw (107); Described movable stand drive motors(106) output shaft drives leading screw (107) to be connected by shaft coupling and movable stand, and movable stand drives leading screw (107) correspondingNut be arranged on movable stand (104);
One end of described blade (201) is provided with magnetic patch (204); The other end of described blade (201) is connected in blade fixed bar(302), described blade fixed bar (302) is fixedly installed on movable stand (104); Described blade upset driver part connectsIn blade (201) middle part; Described blade upset driver part is arranged on movable stand (104); Described blade upset drive divisionPart comprises blade connecting rod (202), kinematic link (206), blade turn drive motor (301); Described blade upset drives electricityThe output shaft clutch disk (203) of machine (301), one end of the distal end connection for transmission connecting rod (206) of disk (203);The bottom of described blade connecting rod (202) is connected on kinematic link (206); The other end of described blade connecting rod (202) connectsConnect blade (201) middle part.
2. full-automatic magnetic separating device according to claim 1, is characterized in that, described bottom bracket comprises microporous pipe bracketAnd main body base (108) (102); Described microporous pipe (101) inserts on microporous pipe bracket (102); Described microporePipe carrier (102) is fixed on main body base (108) by bracket holddown groove (103).
3. full-automatic magnetic separating device according to claim 1, is characterized in that, described movable stand (104) passes through movable standGuide-track groove (105) is embedded in bottom bracket; Described movable stand guide-track groove (105) is slip guiding device or the guiding dress that rollsPut.
4. full-automatic magnetic separating device according to claim 1, is characterized in that, described movable stand (104) and bottom bracket itBetween be connected with buffer spring (205).
5. according to arbitrary described full-automatic magnetic separating device in claim 1 to 4, it is characterized in that described magnetic patch (204)For permanent magnet or electromagnet.
6. according to the full-automatic magnetic separating device described in claim 5, it is characterized in that, the material of described permanent magnet is selected from your ironOne in boron magnets, permanent ferrite, soft magnetic ferrite, SmCo magnetic material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410105196.0A CN103878061B (en) | 2014-03-21 | 2014-03-21 | A kind of full automatic magnetic separating device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410105196.0A CN103878061B (en) | 2014-03-21 | 2014-03-21 | A kind of full automatic magnetic separating device |
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| CN103878061A CN103878061A (en) | 2014-06-25 |
| CN103878061B true CN103878061B (en) | 2016-05-25 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104122127A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Biological sample treatment device |
| CN104297039B (en) * | 2014-09-25 | 2017-01-11 | 深圳市奥特库贝科技有限公司 | Novel magnetic separation mechanism |
| CN106881575B (en) * | 2017-03-17 | 2023-07-04 | 中国东方电气集团有限公司 | Full-automatic sorting and feeding mechanism of assembly robot |
| CN113308364A (en) * | 2021-06-09 | 2021-08-27 | 北京京东方技术开发有限公司 | Nucleic acid extraction substrate, nucleic acid extraction apparatus, and nucleic acid extraction method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP0479448A3 (en) * | 1990-10-02 | 1992-12-23 | Beckman Instruments, Inc. | Magnetic separation device |
| US7597520B2 (en) * | 2005-05-24 | 2009-10-06 | Festo Corporation | Apparatus and method for transferring samples from a source to a target |
| TWI415770B (en) * | 2006-01-13 | 2013-11-21 | Universal Bio Research Co Ltd | Variable form dispensing tube, deformation type dispensing device and deformation type dispensing processing method |
| CN100562739C (en) * | 2007-08-06 | 2009-11-25 | 浙江大学 | Target automatic separating apparatus based on immunomagnetic beads |
| JP2009066476A (en) * | 2007-09-11 | 2009-04-02 | Tamagawa Seiki Co Ltd | Method and device for collecting/dispersing magnetic particle in liquid |
| EP3663004B1 (en) * | 2009-05-15 | 2022-01-26 | Gen-Probe Incorporated | Method and apparatus for performing a magnetic separation procedure |
| EP2488303B1 (en) * | 2009-10-16 | 2017-03-15 | Promega Corporation | Heating, shaking, and magnetizing apparatus |
| US9187725B2 (en) * | 2010-04-30 | 2015-11-17 | Bioneer Corporation | Automatic biological sample purification apparatus equipped with magnetic filed applying part, method of extracting target substance from biological sample, and protein expression and purification method |
| CN203750693U (en) * | 2014-03-21 | 2014-08-06 | 东南大学 | Full-automatic magnetic separation device |
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