CN204027892U - A kind of biological specimen treating apparatus - Google Patents
A kind of biological specimen treating apparatus Download PDFInfo
- Publication number
- CN204027892U CN204027892U CN201420400226.6U CN201420400226U CN204027892U CN 204027892 U CN204027892 U CN 204027892U CN 201420400226 U CN201420400226 U CN 201420400226U CN 204027892 U CN204027892 U CN 204027892U
- Authority
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- China
- Prior art keywords
- flexible
- magnetosheath
- bar magnet
- deep
- well plates
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 239000006249 magnetic particle Substances 0.000 claims abstract description 11
- 230000000694 effects Effects 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 2
- 239000011324 bead Substances 0.000 description 9
- 230000005291 magnetic effect Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 7
- 239000000523 sample Substances 0.000 description 7
- 238000007885 magnetic separation Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 238000005336 cracking Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000001095 motoneuron effect Effects 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 238000007664 blowing Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of biological specimen treating apparatus, for the sample processing apparatus of magnetic-particle, it is characterized in that: this biological specimen treating apparatus comprises the shell (110) of hollow, be positioned at the deep-well plates (108) of shell, flexible bar magnet (106), flexible magnetosheath (107), support (105), air pump (101), reversal valve (102), bar magnet tracheae (103), magnetosheath tracheae (104); Shell (110) is provided with bottom surface (114) and the end face (116) relative with bottom surface, connect the first side (113), the second side (112) and the 3rd side (115) of bottom surface and end face, and the first side is connected with the second side, the second side is connected with the 3rd side.The utility model is simple in structure, is easy to integrated, to reduce pollution feature.
Description
Technical field
The utility model relates to a kind of biological specimen treating apparatus, is specially adapted to the sample processing apparatus based on magnetic-particle, and this device can be for the processing of the biological specimens such as nucleic acid extraction, cell separation, protein or antibody.
Background technology
At biological, field of medicaments, often to process biological specimen, as cracking, mix, the operation such as transfer, the biological specimen processing mode of conventional manual has the shortcomings such as efficiency is low, consuming time, sample contamination, and most of automated biological sample process mode has complex structure, volume is large, cost is high, it is integrated to be not easy to or easily causes the shortcomings such as cross pollution.Biological specimen processing mode based on magnetic-particle, has that efficiency is high, output is large, self-reacting device is reliable and stable, easy to use, and directly carries out the advantages such as pcr amplification, but it is large to have volume, is not suitable for the shortcoming such as integrated.
Utility model content
Technical matters: for the problems referred to above, the utility model provides a kind of biological specimen treating apparatus.Its according to program can automatically complete cracking to biological specimen, mix, the operation such as transfer.
Utility model content: for solving the problems of the technologies described above, the utility model provides a kind of biological specimen treating apparatus, for the sample processing apparatus of magnetic-particle, this biological specimen treating apparatus comprises the shell of hollow, is positioned at the deep-well plates of shell, flexible bar magnet, flexible magnetosheath, support, air pump, reversal valve, bar magnet tracheae, magnetosheath tracheae;
The end face that shell is provided with bottom surface and is oppositely arranged with bottom surface, connects the first side, the second side and the 3rd side of bottom surface and end face, and the first side is connected with the second side, and the second side is connected with the 3rd side;
Deep-well plates is located at bottom surface, and flexible bar magnet is above deep-well plates and vertical with deep-well plates; Flexible magnetosheath and flexible magnetosheath are positioned at the side of flexible bar magnet near deep-well plates; Flexible bar magnet and flexible magnetosheath are fixed on support; Air pump is fixed on the first side; Bar magnet tracheae is connected with support; Reversal valve is connected with bar magnet tracheae respectively at air pump;
Flexible bar magnet is flexible under air pump and reversal valve effect, and in flexible magnetosheath, turnover or drive telescopic enter deep-well plates;
Flexible magnetosheath and flexible bar magnet are flexible under air pump and reversal valve effect, successively or in deep-well plates, pass in and out simultaneously.
Described flexible bar magnet comprises magnet, be positioned at magnet top and the draw-tube being connected with magnet, the bar magnet pore that is positioned at draw-tube inside.
Described flexible magnetosheath comprises flexible tube head, be positioned at flexible tube head top and the double-deck draw-tube being connected with flexible tube head, the magnetosheath pore of being located at double-deck draw-tube.
Described draw-tube or double-deck draw-tube adopt flexible material, and outside surface is with ripple.
Beneficial effect: adopt such scheme, can complete biological specimen processing, as cracking, mix, shift, etc. operation.Under motor effect, flexible bar magnet and flexible magnetosheath be accurately motion (transfer) between deep hole plate hole; Under the acting in conjunction of reversal valve, air pump, flexible bar magnet and flexible magnetosheath motion successively in deep hole plate hole (absorption or release magnetic bead) or move up and down (mixing) simultaneously.
Support level motion, in conjunction with flexible bar magnet or flexible magnetosheath stretching motion, can realize the transfer of sample.
This technology adopts magnetic bead isolation technics, transfer be magnetic bead and on-liquid, avoid in liquid mode, may occurring moving, the recovery causing because of loss of liquid in transfer process reduces; Whole device adopts full-closed structure to pollute to reduce; In addition, this device adopts air pump and draw-tube, has reduced volume, simplifies the structure, and it is easier to integrated.
Brief description of the drawings
Fig. 1 is internal structural map of the present utility model.
Fig. 2 is draw-tube bar magnet internal structural map.
Fig. 3 is draw-tube magnetosheath internal structural map.
Fig. 4 is schematic diagram of the present utility model.
Figure 51 is the 1-initial position of magnetic separation: the position zero hour of sample process, and flexible magnetosheath is positioned at above deep-well plates and not at deep hole plate hole, and flexible bar magnet is positioned at flexible magnetosheath top and not at flexible magnetosheath, magnetic-particle is positioned at deep-well plates.
The 2-of Figure 52 magnetic separation mixes position: the position in some moment in blending process, and flexible magnetosheath extends in deep-well plates, after vibration mixes, is dispersed in deep hole plate hole, and flexible bar magnet is still positioned at initial position.
The 3-adsorbed state of Figure 53 magnetic separation: the position in some moment in magnetic bead adsorption process, flexible bar magnet and flexible magnetosheath lie along deep-well plates bottom, and flexible bar magnet is positioned at flexible magnetosheath bottom.Magnetic-particle is attracted to the lower surface of flexible magnetosheath.
The 4-transferring position of Figure 54 magnetic separation: the position in some moment in magnetic bead transfer process, flexible bar magnet and flexible magnetosheath shrink simultaneously, be positioned at above deep-well plates, and not in deep hole plate hole, magnetic-particle are adsorbed on flexible magnetosheath surface always.
The 5-off-position of Figure 55 magnetic separation: the position in some moment in magnetic bead dispose procedure, after flexible bar magnet separates in deep-well plates with flexible magnetosheath, flexible bar magnet leaves flexible magnetosheath, and flexible magnetosheath is positioned at deep-well plates, and magnetic-particle is released in deep-well plates.
Fig. 6 is deep-well plates planimetric map.
In figure, have: pump 101, reversal valve 102, bar magnet tracheae 103, magnetosheath tracheae 104, support 105, draw-tube bar magnet 106, draw-tube magnetosheath 107, deep-well plates 108, guide rail 109, shell 110, motor 111, bottom surface 112, the first side 113, the second side 112, the 3rd side 115, end face 116, flexible tube head 201, double-deck draw-tube 202, magnetosheath pore 203, magnet 301, draw-tube 302, bar magnet pore 303, air pump P1-401, air pump P2-402, solenoid valve 1-403, solenoid valve 2-404, magnetic-particle 501.
Embodiment
Below in conjunction with drawings and the embodiments, the utility model patent is described in further detail:
Taking paramagnetic particle method nucleic acid extraction as example, specifically tell about the realization of the utility model device below:
As shown in Figure 1, the biological specimen treating apparatus that the utility model provides, be mainly used in the sample processing apparatus of magnetic-particle, this biological specimen treating apparatus comprises the shell 110 of hollow, is positioned at the deep-well plates 108 of shell, flexible bar magnet 106, flexible magnetosheath 107, support 105, air pump 101, reversal valve 102, bar magnet tracheae 103, magnetosheath tracheae 104;
Shell 110 can be partly to put formula, the end face 116 that is provided with bottom surface 114 and is oppositely arranged with bottom surface, connect the first side 113, the second side 112 and the 3rd side 115 of bottom surface and end face, and the first side is connected with the second side, the second side is connected with the 3rd side; Also can be fully closed.
Deep-well plates 108 is located at bottom surface, and flexible bar magnet 106 is above deep-well plates 108 and vertical with deep-well plates 108; Flexible magnetosheath 107 is positioned at the side of flexible bar magnet 106 near deep-well plates 108; Flexible bar magnet 106 and flexible magnetosheath are fixed on support 105; Air pump 101 is fixed on the first side; Bar magnet tracheae 103 is connected with support 105; Reversal valve 102 is connected with bar magnet tracheae 103 respectively at air pump 101;
Flexible bar magnet 106 is flexible under air pump 101 and reversal valve 102 effects, can in flexible magnetosheath 107, pass in and out or drive telescopic 107 to enter deep-well plates 108.
Described flexible magnetosheath 106 and flexible bar magnet 107 are flexible under air pump 101 and reversal valve 102 effects, can in deep-well plates 108, pass in and out successively or simultaneously.
Flexible bar magnet 106 comprises magnet 301, be positioned at magnet 301 tops and the draw-tube 302 being connected with magnet 301, the bar magnet pore 303 that is positioned at draw-tube 302 inside.
Flexible magnetosheath comprises flexible tube head 201, be positioned at flexible tube head 201 tops and the double-deck draw-tube 202 being connected with flexible tube head 201, the magnetosheath pore 203 of being located at double-deck draw-tube 202.
Draw-tube 302 or double-deck draw-tube 202 adopt flexible material, and outside surface is with ripple.
Under motor drives, support drives flexible bar magnet and the tangential movement simultaneously of flexible magnetosheath; Reversal valve gating bar magnet tracheae, air pump P1 is communicated with flexible bar magnet, and under air pump drives, flexible bar magnet is flexible to move up and down, reversal valve gating magnetosheath tracheae simultaneously, flexible bar magnet also can drive magnetosheath to move downward together; Reversal valve gating magnetosheath tracheae, air pump P2 is communicated with flexible magnetosheath, and under air pump drives, flexible bar magnet is flexible to move up and down; Reversal valve gating magnetosheath tracheae and flexible bar magnet are under air pump drives, and air pump P1 is communicated with flexible magnetosheath, and air pump P2 is communicated with flexible bar magnet, and magnetosheath tracheae and flexible bar magnet simultaneous retractable move up and down.
As Fig. 6,8 Kong Weiwei stations 1 of the 1st vertical setting of types of deep-well plates 108, i.e. numbering 1 in deep-well plates.To the namely operation to vertical 8 holes simultaneously of the operation of station 1.In conventional nucleic acid extraction kit: station 1 is lysate, and station 2 is magnetic bead, and station 3 is cleaning fluid I, and station 4 and 5 is cleaning fluid II, and station 6 is eluent.
Flexible magnetosheath, flexible bar magnet are initially position shown in Figure 51, and draw-tube and double-deck draw-tube are in compressive state, under motor effect, support navigates to station 2, reversal valve gating bar magnet tracheae and magnetosheath tracheae, under air pump P1 effect, flexible bar magnet moves to flexible magnetosheath, in the time that flexible bar magnet presses close to stretch magnetosheath bottom, under air pump P2 and P2 acting in conjunction, flexible bar magnet and flexible magnetosheath enter kit desired location, and magnetic bead is adsorbed on flexible magnetosheath bottom under the effect of magnet, as Figure 53.Reversal valve gating bar magnet tracheae and magnetosheath tracheae, under the getter action of air pump P1 and P2, flexible bar magnet and flexible magnetosheath shrink, leave deep-well plates to desired location simultaneously, as Figure 54, under motor effect, support navigates to station 1, and under the air blowing effect of air pump P1 and P2, flexible bar magnet and flexible magnetosheath extend, enter deep-well plates desired location simultaneously, reversal valve gating bar magnet tracheae, under the getter action of air pump P1, flexible bar magnet shrinks, leave flexible magnetosheath and deep-well plates to desired location, as Figure 55.Reversal valve gating magnetosheath tracheae, air pump P2 presses certain frequency and speed air blowing and air-breathing, flexible magnetosheath up-down vibration, magnetic bead is released in solution, realizes sample and mixes, as Figure 52.Reversal valve gating magnetosheath tracheae, under the getter action of air pump P2, flexible bar magnet shrinks, and leaves deep-well plates to desired location, gets back to position as shown in Figure 51.Repeat above-mentioned steps, navigate to station 3,4,5, complete respectively 3 times and clean and elution step.
Last institute should be noted that; above-described embodiment is preferably embodiment of the utility model; but embodiment of the present utility model is not restricted to the described embodiments; wherein structure, the connected mode etc. of each parts all can change to some extent; other any do not deviate from equivalents, improvement, the modification done under Spirit Essence of the present utility model and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection domain of the present utility model.
The above is only preferred implementation of the present utility model; be noted that for those skilled in the art; do not departing under the prerequisite of the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. a biological specimen treating apparatus, for the sample processing apparatus of magnetic-particle, it is characterized in that: this biological specimen treating apparatus comprises the shell (110) of hollow, be positioned at the deep-well plates (108) of shell, flexible bar magnet (106), flexible magnetosheath (107), support (105), air pump (101), reversal valve (102), bar magnet tracheae (103), magnetosheath tracheae (104);
The end face (116) that shell (110) is provided with bottom surface (114) and is oppositely arranged with bottom surface, connect the first side (113), the second side (112) and the 3rd side (115) of bottom surface and end face, and the first side is connected with the second side, the second side is connected with the 3rd side;
Deep-well plates (108) is located at bottom surface, and flexible bar magnet (106) is in deep-well plates (108) top and vertical with deep-well plates (108); Flexible magnetosheath (107) is positioned at the side of flexible bar magnet (106) near deep-well plates (108); Flexible bar magnet (106) and flexible magnetosheath (107) are fixed on support (105); Air pump (101) is fixed on the first side; Bar magnet tracheae (103) is connected with support (105); Reversal valve (102) is connected with bar magnet tracheae (103) respectively at air pump (101);
Flexible bar magnet (106) is flexible under air pump (101) and reversal valve (102) effect, and in flexible magnetosheath (107), turnover or drive telescopic (107) enter deep-well plates (108);
Flexible magnetosheath (106) and flexible bar magnet (107) are flexible under air pump (101) and reversal valve (102) effect, pass in and out successively or simultaneously in deep-well plates (108).
2. biological specimen treating apparatus according to claim 1, it is characterized in that, flexible bar magnet (106) comprises magnet (301), is positioned at magnet (301) top and the draw-tube (302) being connected with magnet (301), is positioned at the inner bar magnet pore (303) of draw-tube (302).
3. biological specimen treating apparatus according to claim 1, it is characterized in that, flexible magnetosheath comprises flexible tube head (201), be positioned at flexible tube head (201) top and the double-deck draw-tube (202) being connected with flexible tube head (201), the magnetosheath pore (203) of being located at double-deck draw-tube (202).
4. according to the biological specimen treating apparatus described in claim 2 or 3, it is characterized in that, draw-tube (302) or double-deck draw-tube (202) adopt flexible material, and outside surface is with ripple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420400226.6U CN204027892U (en) | 2014-07-18 | 2014-07-18 | A kind of biological specimen treating apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420400226.6U CN204027892U (en) | 2014-07-18 | 2014-07-18 | A kind of biological specimen treating apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN204027892U true CN204027892U (en) | 2014-12-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420400226.6U Expired - Lifetime CN204027892U (en) | 2014-07-18 | 2014-07-18 | A kind of biological specimen treating apparatus |
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| Country | Link |
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| CN (1) | CN204027892U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122127A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Biological sample treatment device |
| CN106680061A (en) * | 2016-12-23 | 2017-05-17 | 中山大学 | Apparatus and method for detecting parasite eggs |
-
2014
- 2014-07-18 CN CN201420400226.6U patent/CN204027892U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104122127A (en) * | 2014-07-18 | 2014-10-29 | 东南大学 | Biological sample treatment device |
| CN106680061A (en) * | 2016-12-23 | 2017-05-17 | 中山大学 | Apparatus and method for detecting parasite eggs |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141217 |