CN212864734U

CN212864734U - Automatic nucleic acid extraction instrument

Info

Publication number: CN212864734U
Application number: CN202021382727.8U
Authority: CN
Inventors: 马明星; 魏其林; 张磊
Original assignee: Kunming Huanji Biological Chip Industry Co ltd
Current assignee: Kunming Huanji Biological Chip Industry Co ltd
Priority date: 2020-07-14
Filing date: 2020-07-14
Publication date: 2021-04-02
Anticipated expiration: 2030-07-14

Abstract

The application discloses nucleic acid automatic extraction appearance includes: a heating rotary table, a magnetic rod sleeve operating mechanism and a deep hole plate; the driving assembly is in driving connection with the heating turntable, and the heating turntable rotates around a central shaft of the heating turntable; a plurality of deep hole plates are arranged on the top surface of the heating turntable at intervals; when a deep hole plate is positioned in the operating area of the magnetic rod sleeve operating mechanism, the heating turntable stops rotating; the bar magnet sleeve pipe operating device includes: the operation post, bar magnet subassembly and thimble assembly, the operation post sets up in one side of heating carousel, and bar magnet subassembly and thimble assembly set up on the operation post towards a lateral wall of heating carousel along Y axial interval. The operation is automatic, rapid and simple, and the deep-hole plate with a plurality of holes and preloaded with extraction reagents is adopted, so that a plurality of samples can be simultaneously detected. And reagent consumables are saved. Can be matched with different types of magnetic bead nucleic acid extraction reagents and can be widely applied to samples of animal and plant tissues, blood, body fluid and the like.

Description

Automatic nucleic acid extraction instrument

Technical Field

The application relates to an automatic nucleic acid extractor, which belongs to the field of biological detection.

Background

The magnetic bead method is a novel nucleic acid extraction technology with nano biological magnetic beads as carriers, nucleic acid molecules can be specifically identified and combined with silicon hydroxyl on the surfaces of the magnetic beads and aggregated or dispersed under the action of an external magnetic field, so that manual operation processes of centrifugation, supernatant extraction and the like in the traditional nucleic acid extraction process are thoroughly eliminated, and the automatic extraction of nucleic acid is realized. The method is widely applied to various fields of clinical disease diagnosis, blood transfusion safety, forensic medicine identification, environmental microorganism detection, food safety detection, molecular biology research and the like.

The magnetic bead method nucleic acid extraction process generally comprises: the cell lysis solution in the magnetic bead method is a protein denaturant, can make the cells of animals and pathogenic microorganisms lyse, and denaturize the protein combined with nucleic acid, and release the nucleic acid, the magnetic bead can specifically adsorb nucleic acid, through washing, remove the protein and other impurities except nucleic acid, and then through the elution solution dissociate the nucleic acid adsorbed on the magnetic bead, obtain the nucleic acid solution with high purity and concentration, and the nucleic acid solution can be used for PCR amplification, enzyme digestion, molecular hybridization, etc.

The nucleic acid extractor is an instrument which automatically finishes the work of extracting the nucleic acid of a sample by using a matched nucleic acid extraction reagent and is divided into two types: one type is large-scale automated, commonly referred to as automated liquid workstations; the other type is a small automatic nucleic acid extractor which automatically finishes the extraction and purification process by using a packaged matched reagent.

The large automatic liquid workstation is high in equipment cost and operation cost, and is suitable for extracting thousands of samples of the same kind at a time, so that the application rate is reduced; the small-sized automatic instrument is convenient to operate and widely applied due to low instrument and equipment cost and low running cost. The existing small-sized automatic nucleic acid extractor has the defects of low nucleic acid extraction efficiency, weak pollution prevention capability and few compatible sample types. The whole instrument is complex in structural design and difficult to control and operate a system.

SUMMERY OF THE UTILITY MODEL

The application provides an automatic nucleic acid extractor, which is used for solving the problems that the small automatic nucleic acid extractor in the prior art has low extraction efficiency and can not prevent the mutual poor pollution among different samples; compatible processing of a few sample types

The application provides a nucleic acid automatic extraction appearance, includes: a heating rotary table, a magnetic rod sleeve operating mechanism and a deep hole plate; the driving assembly is in driving connection with the heating turntable, and the heating turntable rotates around a central shaft of the heating turntable;

a plurality of deep hole plates are arranged on the top surface of the heating turntable at intervals; when a deep hole plate is positioned in the operating area of the magnetic rod sleeve operating mechanism, the heating turntable stops rotating;

the bar magnet sleeve pipe operating device includes: the operation column is arranged on one side of the heating turntable, and the magnetic rod assemblies and the sleeve pipe assemblies are arranged on one side wall of the operation column, which faces the heating turntable, at intervals along the Y-axis direction;

each driving assembly of the magnetic rod sleeve operating mechanism is respectively in driving connection with the magnetic rod assembly and the sleeve assembly and respectively drives the magnetic rod assembly and the sleeve assembly to reciprocate along the Y-axis direction;

the bar magnet subassembly includes: the bar magnet, sleeve subassembly includes: and the magnetic rod sleeve is inserted into the opening of the deep hole plate after the magnetic rod is inserted into the magnetic rod sleeve.

Preferably, the heating turntable comprises: the rotary table and the heater are in heat conduction connection with the rotary table.

Preferably, the heating turntable comprises a turntable motor, a driving gear and a gear seat; a rotating shaft is arranged in the central area of the bottom surface of the rotating disc and perpendicular to the rotating disc, and a driving gear is sleeved on one end of the rotating shaft extending out of the bottom surface of the rotating disc; the outer wall of the driving gear is in meshed driving connection with an output shaft of the turntable motor;

the driving gear is rotatably arranged on the gear seat.

Preferably, the heating turntable comprises: the positioning blocking pieces are arranged on the bottom surface of the turntable and are opposite to the deep hole plates;

the positioning sensor is arranged on one side of the turntable, and the positioning baffle sheet is movably clamped and abutted against the sensor end of the positioning sensor;

the positioning sensor is electrically connected with the turntable motor.

Preferably, the bar magnet assembly comprises: the magnetic rod mounting plate is arranged on the bottom surface of the magnetic rod mounting plate in an array manner, and one side of the magnetic rod mounting plate is arranged on the magnetic rod sliding seat;

the sleeve assembly includes: the magnetic rod sleeve is arranged on the bottom surface of the sleeve mounting plate in an array manner, and the sleeve sliding seat is arranged on one side of the sleeve mounting plate;

a plurality of through holes are formed in the sleeve mounting plate in a penetrating mode, and the through sleeve mounting plate is opposite to the inlet end of the magnetic rod sleeve.

Preferably, a lifting guide rail is arranged on one side wall of the operation column facing the heating turntable; the magnetic bar sliding seat and the sleeve sliding seat slide along the lifting guide rail;

the magnetic bar sliding seat is arranged above the sleeve sliding seat.

Preferably, the method comprises the following steps: the magnetic rod screw rod driving assembly and the sleeve screw rod driving assembly are arranged on the other side wall of the operation column at intervals;

the magnetic bar screw rod driving assembly is in driving connection with the magnetic bar sliding seat;

the sleeve screw rod driving assembly is in driving connection with the sleeve sliding seat.

Preferably, the method comprises the following steps: the rotating disc Y-direction lifting mechanism is arranged between the operating column and the heating rotating disc;

carousel Y includes to elevating system: the side wall of the rack moves up and down along a sliding chute arranged at the lower part of one side wall of the operation column;

the first end of the rack is in driving connection with a lifting motor;

the second end of the rack is provided with a supporting seat which extends towards the heating turntable and is connected with the bottom surface of the heating turntable.

Preferably, the method comprises the following steps: the ultraviolet disinfection module is arranged in the shell, and the shell cover is arranged above the heating turntable and the magnetic bar sleeve operating mechanism;

the heating turntable and the magnetic rod sleeve operating mechanism are positioned in the disinfection range of the ultraviolet disinfection module.

Preferably, the method comprises the following steps: the heating turntable is rotatably arranged on the bottom plate; the magnetic rod sleeve operating mechanism is arranged on the bottom plate.

The beneficial effects that this application can produce include:

1) the application provides a nucleic acid automatic extraction appearance, the operation is automatic and quick, simple and convenient, adopts the pre-installation cell culture plate that has a plurality of holes, can operate a plurality of sample detections simultaneously. And reagent consumables are saved. Can be matched with different types of magnetic bead nucleic acid extraction reagents and can be widely applied to samples of animal and plant tissues, blood, body fluid and the like.

2) The application provides a nucleic acid automatic extraction appearance through setting up temperature monitoring module, realizes the constant temperature function to guarantee to draw the best reaction temperature among the purification process, operation interface is friendly, and is simple understandable, need not external computer, need not special training, and the outward appearance is small and exquisite, and the material is firm, and design life is long. The structure design is exquisite, easy operation has characteristics such as save time, laborsaving, high-efficient and good uniformity.

3) The application provides a nucleic acid automatic extraction appearance, adopt step motor to pass through microcomputer control, realized reaching up to 0.1 millimeter's precision drive, its bar magnet surface magnetic field intensity is big, can be with the particle size at 0.03 mu m be less than or equal to D be less than or equal to 10 mu m between ferromagnetism or the ferrous magnetism magnetic bead interact accomplish the magnetic bead removal transfer function of settlement, this product combines together with the magnetic bead nucleic acid purification kit that satisfies the magnetic bead requirement, realize the magnetic bead through the motion of control bar magnet and magnetic sleeve and collect, release, shift, and then accomplish the whole extraction process of nucleic acid.

Drawings

FIG. 1 is a schematic perspective view of an automatic nucleic acid extractor provided herein;

FIG. 2 is a schematic diagram of the front view of the automatic nucleic acid extractor provided in the present application; the X-axis and Y-axis in this application are shown in FIG. 2;

FIG. 3 is a schematic diagram of the right side view of the automatic nucleic acid extractor provided herein;

illustration of the drawings:

10. a base plate; 20. an operating column; 210. a lifting guide rail; 220. a magnetic bar sliding seat; 221. a magnetic bar mounting plate; 222. a magnetic bar; 231. a sleeve slide carriage; 233. a sleeve mounting plate; 232. a magnetic rod sleeve; 211. a magnetic bar screw rod driving assembly; 212. a sleeve screw rod driving assembly; 30. a turntable; 31. a deep hole plate; 311. opening the mouth; 321. positioning a baffle plate; 320. a positioning sensor; 33. a turntable motor; 331. A drive gear; 332. a gear seat; 40. a Y-direction lifting mechanism of the turntable; 410. a lifting motor; 411. A rack; 412. and (4) supporting the base.

Detailed Description

The present application will be described in detail with reference to examples, but the present application is not limited to these examples.

Referring to fig. 1, the present application provides an automatic nucleic acid extractor, comprising: the heating turntable 30, the magnetic rod sleeve 232 operating mechanism and the deep hole plate 31; the driving component is in driving connection with the heating turntable 30, and the heating turntable 30 rotates around the central shaft thereof; a plurality of deep hole plates 31 are arranged on the top surface of the heating turntable 30 at intervals; when a deep hole plate 31 is positioned in the operation area of the magnetic rod sleeve 232 operation mechanism, the heating turntable 30 stops rotating;

the bar magnet sleeve 232 operating mechanism includes: the operation column 20, the magnetic rod 222 assembly and the sleeve assembly, the operation column 20 is arranged at one side of the heating turntable 30, and the magnetic rod 222 assembly and the sleeve assembly are arranged on one side wall of the operation column 20 facing the heating turntable 30 at intervals along the Y-axis direction; each driving component of the magnetic rod sleeve 232 operating mechanism is respectively in driving connection with the magnetic rod 222 component and the sleeve component and respectively drives the magnetic rod 222 component and the sleeve component to reciprocate along the Y-axis direction; the magnetic rod 222 assembly comprises a magnetic rod 222, the sleeve assembly comprises a magnetic rod sleeve 232, and the magnetic rod 222 is inserted into the liquid opening 311 of the deep hole plate 31 after being inserted into the magnetic rod sleeve 232.

Through setting up the heating carousel 30 that can heat deep hole board 31, the deep hole board 31 intussuseption on heating carousel 30 surface is filled with and is waited to extract nucleic acid solution, through heating plate heating back, is convenient for the normal position after realizing protein denaturation schizolysis nucleic acid and accomplishes the magnetic bead nucleic acid extraction process. After the heating turntable 30 rotates to the operating position each time, the rotation is stopped, and the magnet bar 222 assembly and the magnet bar sleeve 232 are sequentially lowered from the deep hole plate 31.

Along with the magnetic rod 222 and the magnetic rod sleeve 232 are inserted into the deep hole plate 31, the stirring vibration of the solution in each hole of the deep hole plate 31 can be realized, the oscillation operation is realized, and the nucleic acid separation efficiency is improved.

The deep well plate 31 used may be a 96 well plate or other well-number cell culture plate. The number of the magnetic rods 222 and the magnetic rod sleeves 232 corresponding to the number of the holes of the cell culture plate is set according to the number of the holes, so that the nucleic acid extraction can be generally realized for each hole. And the number of holes of each deep hole plate 31 is the same.

The connection mode of the driving component, the heating turntable 30 and the magnetic rod sleeve 232 operating mechanism can be set according to the existing setting mode. The deep hole plates 31 may be spaced apart from each other in a radial direction of the heating turntable 30.

The deep hole plate 31 is an existing deep hole plate 31, and a plurality of openings 311 are formed in the top surface of the deep hole plate 31, so that the magnetic rods 222 can conveniently penetrate into the openings 311 and contact with the solution.

Referring to fig. 1-2, preferably, the heating turntable 30 includes: the turntable 30, a heater, the heater is connected with the turntable 30 in a heat conduction way. The heat generated by the heater is conducted to each deep hole plate 31 through the turntable 30.

Preferably, the heating turntable 30 includes a turntable motor 33, a driving gear 331, a gear seat 332; a rotating shaft is arranged in the central area of the bottom surface of the rotating disc 30 and is vertical to the rotating disc 30, and a driving gear 331 is sleeved on one end of the rotating shaft extending out of the bottom surface of the rotating disc 30; the outer wall of the driving gear 331 is in meshing driving connection with the output shaft of the turntable motor 33;

the driving gear 331 is rotatably mounted on the gear holder 332.

According to the arrangement, the turntable 30 can rotate around the axis of the turntable 30, the stability of the rotating object of the turntable 30 can be improved by arranging the gear seat 332, and the extraction operation is prevented from being influenced by the inclination of the deep hole plate 31. The gear seat 332 is disposed below the turntable 30 and perpendicular to the turntable 30, so as to support the whole turntable 30.

The heater may be disposed in an interlayer disposed in the turntable 30, or may be disposed on the bottom surface of the turntable 30 and in heat-conductive contact with the turntable 30.

Preferably, the heating dial 30 includes: the positioning sensor 320 and a plurality of positioning blocking pieces 321, the positioning blocking pieces 321 are arranged on the bottom surface of the turntable 30 and are opposite to the deep hole plates 31; the positioning sensor 320 is arranged at one side of the turntable 30, and the positioning baffle piece 321 is movably clamped and abutted against the sensor end of the positioning sensor 320; the position sensor 320 is electrically connected to the turntable motor 33.

According to the arrangement, when the turntable 30 rotates and the deep hole plate 31 is opposite to the magnetic rod sleeve 232 operating mechanism, the positioning blocking piece 321 is blocked in the positioning sensor 320, the positioning sensor 320 sends a control signal to the turntable motor 33 to control the turntable motor 33 to stop rotating, so that the magnetic rod 222 is extracted. By adopting the mode, the rotation amplitude of the turntable 30 can be accurately controlled, and the condition that the deep hole plate 31 cannot be operated due to the fact that the deep hole plate rotates through an operation area is avoided. The extraction efficiency is improved, and the extraction precision is ensured.

Preferably, the bar magnet 222 assembly includes: the magnetic rod sliding seat 220, the magnetic rod mounting plate 221 and the plurality of magnetic rods 222, wherein the magnetic rods 222 are mounted on the bottom surface of the magnetic rod mounting plate 221 in an array manner, and one side of the magnetic rod mounting plate 221 is arranged on the magnetic rod sliding seat 220;

the sleeve assembly includes: the magnetic rod sleeving device comprises a sleeving sliding seat 231, a sleeving mounting plate 233 and a plurality of magnetic rod sleeving 232, wherein the magnetic rod sleeving 232 is mounted on the bottom surface of the sleeving mounting plate 233 in an array mode, and the sleeving sliding seat 231 is arranged on one side of the sleeving mounting plate 233;

the sleeve mounting plate 233 is provided with a plurality of through holes which penetrate through the sleeve mounting plate 233 and are opposite to the inlet end of the magnetic rod sleeve 232.

According to the arrangement, when the magnetic rod 222 descends, the magnetic rod 222 can be inserted or removed in alignment with the magnetic rod sleeve 232, so that the elution operation of denatured protein is realized, and the extraction efficiency is improved.

Preferably, the lifting guide rail 210 is arranged on a side wall of the operation column 20 facing the heating turntable 30; the magnetic rod sliding base 220 and the sleeve sliding base 231 slide along the lifting guide rail 210; the magnetic rod slider 220 is disposed above the sleeve slider 231.

According to the arrangement, the magnetic rods 222 arranged in an array mode can be accurately inserted into the magnetic rod sleeve 232, and the accuracy of automatic operation is improved.

Referring to fig. 3, preferably, it includes: the magnetic rod screw driving assembly 211 and the sleeve screw driving assembly 212 are arranged on the other side wall of the operation column 20 at intervals; the magnetic bar screw rod driving component 211 is in driving connection with the magnetic bar sliding seat 220; the quill screw drive assembly 212 is drivingly connected to the quill slide 231.

The used lead screw is a ball screw, so that the accurate control of the descending of the magnetic rod 222 and the magnetic rod 222 sleeve can be realized, the operation precision is improved, and the automatic operation is realized while the high-efficiency extraction is realized. The connection of the drive spindle to the carriages is provided in the prior art.

Referring to fig. 1-2, preferably, includes: a turntable Y-direction lifting mechanism 40, wherein the turntable Y-direction lifting mechanism 40 is arranged between the operating column 20 and the heating turntable 30; the turntable Y-direction lifting mechanism 40 includes: the lifting motor 410, the rack 411 and the supporting seat 412, wherein the side wall of the rack 411 moves up and down along a sliding groove arranged at the lower part of one side wall of the operating column 20; the first end of the rack 411 is in driving connection with the lifting motor 410; the second end of the rack 411 is provided with a support seat 412 by a vertical rack 411, and the support seat 412 extends towards the heating rotary disc 30 and is connected with the bottom surface of the heating rotary disc 30.

According to the arrangement, when heating is needed, in order to avoid adverse effects on the turntable motor 33, the heating turntable 30 is lifted integrally to the lifting mechanism 40 through the turntable Y to be heated, and the service life of the turntable motor 33 can be prolonged. However, when the heating is completed and the rotation is required, the rack 411 may be driven to descend by the elevating motor 410 and then rotated.

Preferably, the ultraviolet disinfection module is arranged in the shell, and the shell is covered above the heating turntable 30 and the magnetic rod sleeve 232 operating mechanism; the heating turntable 30 and the magnetic rod sleeve 232 operating mechanism are in the disinfection range of the ultraviolet disinfection module.

According to the arrangement, when the ultraviolet lamp is not used, the main components in the instrument can be disinfected through the ultraviolet lamp. The device can also be arranged in a timing control module to realize the control of the disinfection time.

A power supply can be arranged for controlling the startup and shutdown conveniently; and meanwhile, a color operation touch screen is arranged, so that the operation control of the instrument is convenient to realize.

Preferably, the heating rotary table comprises a bottom plate 10, and the heating rotary table 30 is rotatably arranged on the bottom plate 10; the magnetic rod sleeve 232 operating mechanism is arranged on the bottom plate 10.

When in use, 1) the reagent to be extracted and the cracking agent are loaded in the plurality of deep hole plates 31, after the turntable Y-direction lifting mechanism 40 is started to lift the turntable 30, the liquid to be extracted in the deep hole plates 31 is heated, so that the protein is denatured to release nucleic acid; the heating time is typically minutes;

2) after heating is completed, the deep hole plate 31 rotates along with the turntable 30, the positioning stop piece 321 is clamped on the positioning sensor 320, the turntable motor 33 stops rotating, and the deep hole plate 31 is located on the operation position of the operation column 20;

3) after the magnetic rod 222 assembly and the sleeve assembly synchronously move downwards along the Y axis and extend into the deep hole plate 31, the magnetic rod 222 assembly drives the magnetic rod 222 to move upwards along the Y axis and leave the magnetic rod sleeve 232, and the magnetic rod sleeve 232 vibrates in the deep hole plate 31, so that the effect of vibrating the solution is realized.

After the oscillation is finished, the sleeve assembly moves upwards to the middle of the operation column 20, and the magnetic rod 222 is inserted into the magnetic rod sleeve 232 after the magnetic rod 222 assembly moves downwards; then the magnetic rod 222 assembly and the sleeve assembly are moved downwards together and inserted into the deep hole plate 31, and the magnetic rod 222 adsorbs nucleic acid after standing.

4) After the adsorption is finished, the turntable motor 33 is started, the turntable 30 rotates, and the next deep hole plate 31 rotates to the operation position and stops rotating; step 2) is repeated, and nucleic acid extraction is performed on another deep well plate 31.

Reference throughout this specification to "one embodiment," "another embodiment," "an embodiment," "a preferred embodiment," or the like, means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the disclosure to effect such feature, structure, or characteristic in connection with other embodiments.

Although the present application has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure and claims of this application. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.

Claims

1. An automatic nucleic acid extractor, comprising: a heating rotary table, a magnetic rod sleeve operating mechanism and a deep hole plate (31); the driving assembly is in driving connection with the heating turntable, and the heating turntable rotates around a central shaft of the heating turntable;

a plurality of deep hole plates (31) are arranged on the top surface of the heating turntable at intervals; when a deep hole plate (31) is positioned in the operating area of the magnetic rod sleeve operating mechanism, the heating turntable stops rotating;

the bar magnet sleeve operating mechanism comprises: the heating rotary table comprises an operation column (20), magnetic rod assemblies and sleeve assemblies, wherein the operation column (20) is arranged on one side of the heating rotary table, and the magnetic rod assemblies and the sleeve assemblies are arranged on one side wall, facing the heating rotary table, of the operation column (20) at intervals along the Y-axis direction;

each driving assembly of the magnetic rod sleeve operating mechanism is in driving connection with the magnetic rod assembly and the sleeve assembly respectively and drives the magnetic rod assembly and the sleeve assembly to reciprocate along the Y-axis direction respectively;

the bar magnet assembly comprises: a magnetic bar (222), the sleeve assembly comprising: the magnetic rod sleeve (232), the magnetic rod (222) is inserted into the opening (311) of the deep hole plate (31) after being inserted into the magnetic rod sleeve (232).

2. The automatic nucleic acid isolation instrument according to claim 1, wherein the heating turntable comprises: the rotary table comprises a rotary table (30) and a heater, wherein the heater is in heat conduction connection with the rotary table (30).

3. The automatic nucleic acid extractor of claim 2, wherein the heating turntable comprises a turntable motor (33), a driving gear (331), a gear seat (332); a rotating shaft is arranged in the central area of the bottom surface of the rotating disc (30) and is vertical to the rotating disc (30), and a driving gear (331) is sleeved at one end of the rotating shaft, which extends out of the bottom surface of the rotating disc (30); the outer wall of the driving gear (331) is in meshed driving connection with an output shaft of the turntable motor (33);

the driving gear (331) is rotatably mounted on the gear seat (332).

4. The automatic nucleic acid isolation instrument according to claim 1, wherein the heating turntable comprises: the positioning sensor (320) and a plurality of positioning blocking pieces (321), wherein the positioning blocking pieces (321) are arranged on the bottom surface of the turntable (30) and are opposite to the deep hole plates (31);

the positioning sensor (320) is arranged on one side of the turntable (30), and the positioning baffle sheet (321) is movably clamped and abutted to the sensor end of the positioning sensor (320);

the positioning sensor (320) is electrically connected with the turntable motor (33).

5. The automatic nucleic acid extractor of claim 1, wherein the magnetic rod assembly comprises: the magnetic rod installing device comprises a magnetic rod sliding seat (220), a magnetic rod installing plate (221) and a plurality of magnetic rods (222), wherein the magnetic rods (222) are arranged on the bottom surface of the magnetic rod installing plate (221) in an array mode, and one side of the magnetic rod installing plate (221) is arranged on the magnetic rod sliding seat (220);

the cannula assembly includes: the magnetic rod sleeving device comprises a sleeve sliding seat (231), a sleeve mounting plate (233) and a plurality of magnetic rod sleeves (232), wherein the magnetic rod sleeves (232) are mounted on the bottom surface of the sleeve mounting plate (233) in an array manner, and the sleeve sliding seat (231) is arranged on one side of the sleeve mounting plate (233);

and a plurality of through holes are formed in the sleeve mounting plate (233) through the sleeve mounting plate (233) and are opposite to the inlet end of the magnetic rod sleeve (232).

6. The automatic nucleic acid extractor of claim 5, wherein the operation column (20) is provided with a lifting guide (210) on a side wall facing the heating turntable; the magnetic rod sliding seat (220) and the sleeve sliding seat (231) both slide along the lifting guide rail (210);

the magnetic rod sliding seat (220) is arranged above the sleeve sliding seat (231).

7. The automatic nucleic acid extractor according to claim 5, comprising: the magnetic rod screw driving assembly (211) and the sleeve screw driving assembly (212) are arranged on the other side wall of the operating column (20) at intervals;

the magnetic rod screw rod driving assembly (211) is in driving connection with the magnetic rod sliding seat (220);

the sleeve screw rod driving assembly (212) is in driving connection with the sleeve sliding seat (231).

8. The automatic nucleic acid extractor according to claim 1, comprising: the Y-direction lifting mechanism (40) of the turntable is arranged between the operating column (20) and the heating turntable;

the Y-direction lifting mechanism (40) of the turntable comprises: the lifting mechanism comprises a lifting motor (410), a rack (411) and a supporting seat (412), wherein the side wall of the rack (411) moves up and down along a sliding groove arranged at the lower part of one side wall of the operating column (20);

the first end of the rack (411) is in driving connection with the lifting motor (410);

and a supporting seat (412) is arranged on the vertical rack (411) at the second end of the rack (411), and the supporting seat (412) extends towards the heating turntable and is connected with the bottom surface of the heating turntable.

9. The automatic nucleic acid extractor according to claim 1, comprising: the ultraviolet disinfection module is arranged in the shell, and the shell cover is arranged above the heating turntable and the magnetic bar sleeve operating mechanism;

10. The automatic nucleic acid extractor according to claim 1, comprising: the heating turntable is rotatably arranged on the bottom plate (10); the magnetic rod sleeve operating mechanism is arranged on the bottom plate (10).