CN108048302B - Full-automatic nucleic acid extractor - Google Patents
Full-automatic nucleic acid extractor Download PDFInfo
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- CN108048302B CN108048302B CN201810102670.2A CN201810102670A CN108048302B CN 108048302 B CN108048302 B CN 108048302B CN 201810102670 A CN201810102670 A CN 201810102670A CN 108048302 B CN108048302 B CN 108048302B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
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Abstract
The invention relates to a full-automatic nucleic acid extraction instrument, which comprises a platform assembly, a nucleic acid extraction assembly and an automatic pipetting assembly which are arranged on a bottom plate, wherein the platform assembly comprises a reagent tank carrier, an EP (European standard) pipe carrier, a blood collection pipe carrier, a first deep pore plate carrier and a TIP head carrier which are arranged on the bottom plate; one side of the platform component is provided with a plurality of second deep hole plates, and the nucleic acid extraction component comprises a nucleic acid extraction arm which can move above the second deep hole plates and can be lifted; the automated pipetting assembly includes a pipetting arm movable and liftable above the nucleic acid extraction assembly and the platform assembly. The invention is mainly formed by integrating a pipetting part, a nucleic acid extraction part and other modules, realizes the full-automatic extraction process from a sample to purified nucleic acid, avoids the interference of manual operation on the result, reduces the labor intensity, reduces the waste of consumables, improves the extraction precision and accelerates the extraction speed.
Description
Technical Field
The invention belongs to the field of medical equipment manufacturing, and particularly relates to a full-automatic nucleic acid extractor.
Background
Nucleic acid is a biological macromolecular compound polymerized from many nucleotides, and is one of the most basic substances for life. Nucleic acids are widely found in all animal and plant cells and microorganisms, and nucleic acids in organisms are often combined with proteins to form nucleoproteins. With the rapid development of molecular biology in recent years, nucleic acid-based molecular diagnostic and detection techniques are increasingly playing a vital role in a variety of fields. The nucleic acids required for the experiments and assays need to be purified from biological samples, but it is quite difficult to purify multiple samples. The process not only needs to select a proper purification technology, but also has extremely large workload, and is difficult to meet the current rapidly-developed requirements of extracting and purifying high-flux samples.
At present, most nucleic acid extractors adopt a magnetic bead method to extract nucleic acid, and the nucleic acid extractor used in the extraction process is an instrument which automatically completes the extraction work of sample nucleic acid by using matched magnetic bead nucleic acid extraction reagents. The existing nucleic acid extractor is divided into two types, namely a large-sized automatic liquid workstation, and nucleic acid is obtained by transferring liquid, so that the full-automatic operation can be realized, and the nucleic acid extractor has the advantages of no special use for extracting nucleic acid, long time consumption and longer time for extracting nucleic acid each time; the other type is a small magnetic rod type nucleic acid extractor, and nucleic acid is obtained by transferring magnetic beads, so that the time is short, 30 to 50 minutes are required, and the disadvantage is that the kit is required to be preloaded or manually packaged on site, the kit has no functions of liquid separation and liquid transfer, and a large number of experimental samples cannot be fully automatically processed.
Disclosure of Invention
In order to solve the problems, the invention provides a full-automatic nucleic acid extractor for efficiently and rapidly obtaining high-purity nucleic acid samples, which comprises the following specific scheme:
the utility model provides a full-automatic nucleic acid extraction appearance, includes platform subassembly, nucleic acid extraction subassembly and the automatic pipetting component of setting on the bottom plate, platform subassembly is used for placing various reagents and containers, reagent and container are in the position on the bottom plate can freely be combined, nucleic acid extraction subassembly is used for magnetic bead transfer formula nucleic acid extraction, automatic pipetting component is used for the platform subassembly with accurate liquid transfer between the nucleic acid extraction subassembly.
The platform assembly comprises a reagent tank carrier, an EP (electronic pressure) pipe carrier, an blood collection pipe carrier, a PCR (polymerase chain reaction) eight-connection EP pipe carrier and a TIP (TIP) head carrier which are arranged on the bottom plate, wherein a reagent tank is arranged on the reagent tank carrier, an EP pipe rack is arranged on the EP pipe carrier, a blood collection pipe is arranged on the blood collection pipe carrier, the PCR eight-connection EP pipe is arranged on the PCR eight-connection EP pipe carrier, and a TIP head frame is arranged on the TIP head carrier.
The nucleic acid extraction assembly comprises a 96 deep hole plate arranged on the bottom plate, a first X-axis movement mechanism arranged above the 96 deep hole plate, a first Z-axis movement mechanism arranged on the first X-axis movement mechanism and a nucleic acid extraction arm arranged on the first Z-axis movement mechanism; the nucleic acid extraction arm is provided with a plurality of magnetic sleeve frames through a first connecting plate, and the magnetic sleeve frames are provided with a plurality of magnetic sleeves which are vertically arranged and can extend into the 96-deep hole plate; the nucleic acid extraction arm is located the top of magnetic sleeve frame is equipped with a plurality of magnetic rod frames through the second connecting plate, be equipped with a plurality of ability on the magnetic rod frame and stretch into the magnetic rod in the magnetic sleeve.
Further, a plurality of heating components are arranged below the 96 deep hole plates, each heating component comprises a heating block, a heating resistor is arranged below each heating block, and a heat insulation sleeve is arranged between each heating resistor and the bottom plate.
Further, a baffle device is further arranged on the bottom plate, the baffle device comprises a first baffle arranged above the 96 deep hole plate, a first rotating shaft and a second rotating shaft which are arranged in parallel are arranged at one end of the first baffle, and the distance between the first rotating shaft and the edge of the first baffle is smaller than the distance between the second rotating shaft and the edge of the first baffle; one end of the first rotating shaft and one end of the second rotating shaft are connected with a driving mechanism used for driving the first baffle plate to turn over, and the driving mechanism can drive the first baffle plate to turn over to the position right above the 96-depth hole plate.
Further, the driving mechanism comprises a baffle shell, the first rotating shaft penetrates through the baffle shell and is connected with a third rotating shaft through a first connecting rod, and the second rotating shaft penetrates through the baffle shell and is connected with a transmission assembly capable of driving the second rotating shaft to do circular arc motion through a second connecting rod; the input end of the transmission assembly is provided with a stepping motor, the baffle shell is provided with a first movement groove for the movement of the first rotation shaft, a second movement groove for the movement of the second rotation shaft is arranged below the first movement groove, the radian of the second movement groove is larger than that of the first movement groove, and when the second rotation shaft moves to the highest position of the second movement groove, the height of the first rotation shaft is the same as that of the second rotation shaft; and a baffle circuit board connected with the stepping motor is further arranged in the baffle shell.
The automatic pipetting component comprises a second X-axis movement mechanism arranged above the nucleic acid extraction component and a first Y-axis movement mechanism arranged on the second X-axis movement mechanism, a pipetting arm is arranged on the first Y-axis movement mechanism, a plurality of pipetting units are arranged on the pipetting arm, and each pipetting unit comprises a second Z-axis movement mechanism arranged on the second Y-axis movement mechanism and a sampling tube arranged on the second Z-axis movement mechanism.
Further, a waste collection device is arranged on the bottom plate, and the waste collection device comprises a TIP head unloading device for assisting the pipetting arm to remove the waste TIP head from the sampling tube; a waste slideway is arranged right below the TIP head unloading device, and a containing device of the waste TIP head is arranged at the bottom of the waste slideway.
Further, a plurality of guide rails which are arranged in parallel are arranged on the EP pipe carrier, clamping grooves matched with the guide rails are formed in the bottom of the EP pipe carrier, and the EP pipe carrier is made of polyoxymethylene materials.
Further, a second baffle plate is wrapped around the TIP head carrier, and a plurality of elastic sheets for clamping the TIP head frame on the TIP head carrier are arranged on the top surface of the TIP head carrier.
The invention has the beneficial effects that: the invention provides a full-automatic nucleic acid extraction device which is mainly formed by integrating a pipetting part, a nucleic acid extraction part and other modules, so that the full-automatic extraction process from a sample to purified nucleic acid is realized, the interference of manual operation on a result is avoided, the labor intensity is reduced, the waste of consumables is reduced, the extraction precision is improved, and the extraction speed is accelerated.
Drawings
Figure 1. The overall structure of embodiment 1 of the present invention is schematically shown,
figure 2 is a schematic view of the structure of the platform assembly according to embodiment 1 of the present invention,
FIG. 3 is a schematic diagram showing the structure of a nucleic acid isolation module according to example 1 of the present invention,
FIG. 4 is a schematic view showing a construction of a pipetting device according to embodiment 1 of the invention,
figure 5. A structural plan view of the baffle device of embodiment 1 of the present invention,
figure 6 is a side view of the structure of the baffle device of embodiment 1 of the present invention,
figure 7 is a schematic view showing the internal structure of the baffle device of embodiment 1 of the present invention,
figure 8 is a schematic view of the heating assembly of embodiment 1 of the present invention,
figure 9. A schematic view of the structure of the EP pipe carrier of example 1 of the present invention,
figure 10 is a schematic view of the structure of a TIP head carrier according to embodiment 1 of the present invention,
fig. 11 is a schematic view showing the structure of a TIP head remover according to embodiment 2 of the present invention.
Figure number and name: 1. a bottom plate, 2, a platform assembly, 201, a reagent tank carrier, 202, an EP tube carrier, 203, a blood collection tube carrier, 204, a PCR eight-link EP tube carrier, 205, a TIP head carrier, 206, a reagent tank, 207, an EP tube carrier, 208, a blood collection tube, 209, a PCR eight-link EP tube, 210, a TIP head carrier, 3, a nucleic acid extraction assembly, 301, a 96 deep well plate, 302, a nucleic acid extraction arm, 303, a first X-axis movement mechanism, 304, a first Z-axis movement mechanism, 305, a first connection plate, 306, a magnetic sleeve carrier, 307, a magnetic sleeve, 308, a second connection plate, 309, a magnetic rod carrier, 310, a magnetic rod, 4, an automatic pipetting assembly, 401, a pipetting arm, 402, a second X-axis movement mechanism, 403, a first Y-axis movement mechanism, 404, second Y-axis movement mechanism, 405, second Z-axis movement mechanism, 406, sampling tube, 5, shutter device, 501, shutter first, 502, driving mechanism, 503, first rotation axis, 504, second rotation axis, 505, shutter housing, 506, first link, 507, third rotation axis, 508, second link, 509, transmission assembly, 510, stepper motor, 511, first movement groove, 512, second movement groove, 513, shutter circuit board, 6, waste collection device, 601, TIP unloading head device, 602, waste slide, 7, heating assembly, 701, heating block, 702, heating resistor, 703, heat insulation sleeve, 8, guide rail, 9, clamping groove, 10, shutter second, 11, and spring sheet.
Detailed Description
The present invention is further described below with reference to the drawings and examples, which are only for explaining the present invention and are not intended to limit the scope of the present invention.
Example 1
Referring to fig. 1 and 2, a full-automatic nucleic acid extraction apparatus includes a platform assembly 2, a nucleic acid extraction assembly 3 and an automatic pipetting assembly 4, which are disposed on a bottom plate 1, wherein the platform assembly 2 includes a reagent tank carrier 201, an EP tube carrier 202, a blood collection tube carrier 203, a PCR eight-link EP tube carrier 204 and a TIP head carrier 205, which are disposed on the bottom plate 1, a reagent tank 206 is disposed on the reagent tank carrier 201, an EP tube rack 207 is disposed on the EP tube carrier 202, a blood collection tube 208 is disposed on the blood collection tube carrier 203, a PCR eight-link EP tube 209 is disposed on the PCR eight-link EP tube carrier 204, and a TIP head frame 210 is disposed on the TIP head carrier 205; the nucleic acid extraction assembly 3 comprises a plurality of 96 deep hole plates 301 arranged on one side of the platform assembly 2, and the nucleic acid extraction assembly 3 also comprises a nucleic acid extraction arm 302 which can move above the 96 deep hole plates 301 and can be lifted; the automatic pipetting assembly 4 comprises a pipetting arm 401 movable and liftable above the nucleic acid extraction assembly 3 and the platform assembly 2.
As shown in fig. 3, the nucleic acid extraction module 3 includes a first X-axis movement mechanism 303 provided above a 96-well plate 301 and a first Z-axis movement mechanism 304 provided on the first X-axis movement mechanism 303, and the nucleic acid extraction arm 302 is provided on the first Z-axis movement mechanism 304; a plurality of magnetic sleeves 306 are arranged on the nucleic acid extraction arm 302 through a first connecting plate 305, and a plurality of magnetic sleeves 307 which are vertically arranged and can extend into the 96 deep hole plate 301 are arranged on the magnetic sleeves 306; the nucleic acid extraction arm 302 is positioned above the magnetic sleeve frame 306, a plurality of magnetic rod frames 309 are arranged on the magnetic rod frames 309 through a second connecting plate 308, and a plurality of magnetic rods 310 which can extend into the magnetic sleeve 307 are arranged on the magnetic rod frames 309; the magnetic rod rack and the magnetic sleeve rack are divided into three groups of two columns of 8 magnetic rods. Compared with a large 48-channel magnetic rod rack, the magnetic rod rack has the advantages that the structure is flexible, the adjustment and the installation are convenient, and the processing difficulty and the processing cost are reduced; the magnetic rod frame and the magnetic sleeve frame are designed in the corner arc mode, so that the hand is scratched by operators when the magnetic sleeve is inserted and taken in the experiment process is avoided, and the risk that the operators are infected is reduced.
Referring to fig. 1 and 4, the automatic pipetting module 4 includes a second X-axis movement mechanism 402 disposed above the nucleic acid extraction module 3 and a first Y-axis movement mechanism 403 disposed on the second X-axis movement mechanism 402, the pipetting arm 401 is disposed on the first Y-axis movement mechanism 403, and a plurality of pipetting units are disposed on the pipetting arm 401, each pipetting unit including a second Z-axis movement mechanism 405 disposed on the second Y-axis movement mechanism 404 and a sampling tube 406 disposed on the second Z-axis movement mechanism 405.
Referring to fig. 5-7, a baffle device 5 is further arranged on the bottom plate 1, the baffle device 5 comprises a first baffle 501 and a driving mechanism 502, which are arranged above the 96 deep hole plate 301, a first rotating shaft 503 and a second rotating shaft 504 are arranged in parallel at one end of the first baffle 501, and the distance between the first rotating shaft 503 and the edge of the first baffle 501 is smaller than the distance between the second rotating shaft 504 and the edge of the first baffle 501; the driving mechanism 502 comprises a baffle shell 505, a first rotating shaft 503 penetrates through the baffle shell 505 and is connected with a third rotating shaft 507 through a first connecting rod 506, and a second rotating shaft 504 penetrates through the baffle shell 505 and is connected with a transmission assembly 509 capable of driving the second rotating shaft 504 to do circular arc motion through a second connecting rod 508; the input end of the transmission component 509 is provided with a stepping motor 510, the baffle shell 505 is provided with a first movement groove 511 for the first rotation shaft 503 to move, a second movement groove 512 for the second rotation shaft 504 to move is arranged below the first movement groove 511, the radian of the second movement groove 512 is larger than that of the first movement groove 511, and when the second rotation shaft 504 moves to the highest position of the second movement groove 512, the height of the first rotation shaft 503 is the same as that of the second rotation shaft 504; a shutter circuit board 513 connected to the stepping motor 510 is also provided in the shutter housing 505.
The bottom plate 1 is also provided with a waste collection device 6, the waste collection device 6 comprises a TIP head unloading device 601 for assisting the pipetting arm 401 to remove the waste TIP head from the sampling tube 406, as shown in fig. 1 and 2, the TIP head unloading device 601 is of a plate-shaped structure fixed on the bottom plate, the top of the TIP head unloading device deflects towards the pipetting arm to form a falling claw, and when the sampling tube clings to the falling claw and then moves upwards, the waste TIP head can fall off; a waste slideway 602 is arranged under the TIP head unloading device 601, an external garbage can for discarding TIP heads is arranged at the bottom of the waste slideway 602, and the fallen waste TIP heads are collected, so that pollution is avoided.
As shown in fig. 8, a plurality of heating assemblies 7 are arranged below the 96 deep hole plates 301, each heating assembly 7 comprises a heating block 701, a heating resistor 702 is arranged below each heating block 701, a heat insulation sleeve 703 is arranged between each heating resistor 702 and the bottom plate 1, and each placement area comprises an independent heating device, so that the heating requirements of different steps in the nucleic acid extraction process on reagents are met. The heating block adopts brass processing, and corner circular arc design has avoided operating personnel to scratch gloves when taking the consumptive material and has infected risk, built-in heating resistor, has advantages such as thermal conduction efficiency is high-speed fast, separates mutually with the insulating sheath between heating block and the platform, and the insulating sheath adopts PEEK material, has that the heat resistance is high, and the machinability is strong, advantages such as corrosion resistance.
As shown in fig. 9, a plurality of parallel guide rails 8 are arranged on the EP pipe carrier 202, clamping grooves 9 matched with the guide rails 8 are arranged at the bottom of the EP pipe rack 207, the drawing type slideway structure is designed to be convenient to take, the EP pipe rack 207 is made of a polyoxymethylene material, the polyoxymethylene material has a self-lubricating effect, and abrasion to the carrier in the drawing process is reduced. As shown in fig. 10, the periphery of the TIP head carrier 205 is covered with the second baffle plates 10, so as to avoid the risk of pollution of the clean TIP head, and the top surface of the TIP head carrier 205 is provided with a plurality of elastic pieces 11 for clamping the TIP head frame 210 on the TIP head carrier 205, so that the TIP head frame is effectively clamped and stabilized, and the TIP head frame is not carried up when the TIP head is picked up by the pipetting arm.
The base plate 1 is also provided with a lighting system, an ultraviolet sterilization system and a control system (not shown in the drawings) for controlling the respective components.
The invention has the dual functions of pipetting and magnetic bars, the inside of the instrument comprises a pipetting arm with 1-8 channels which are independently lifted and a high-strength magnetic bar with 1-48 channels, and the automatic transfer, suction, vibration and other actions of liquid and magnetic beads can be carried out by matching with consumable materials such as a reagent tank, a centrifuge tube, a 96 deep hole plate, a PCR eight-link EP tube, a magnetic sleeve and the like, so that the full-automatic operation of a sample preparation link is realized.
Example 2
Referring to fig. 11, this embodiment differs from embodiment 1 in that:
the TIP head unloading device 601 of this embodiment is a TIP head removing blocking piece mounted on a pipetting arm, when the pipetting unit drives the sampling tube to move upwards, when the TIP head moves to the position of the TIP head removing blocking piece, the waste TIP head can automatically drop from the sampling tube and then drop into a waste slideway below. The working process is more flexible compared with the working process of the embodiment 1.
The working process of the invention is as follows:
firstly, self-preparing, putting reagents, biological samples, TIP heads, 96 deep well plates, PCR eight-link EP pipes and the like at corresponding positions;
then, the automatic pipetting device automatically installs TIP, and sequentially adds the reagent in the reagent tank and the biological sample in the blood collection tube on the platform module into the deep hole plate on the nucleic acid extraction platform, and the automatic nucleic acid extraction device completes the extraction and purification of nucleic acid;
then, the automatic pipetting device transfers the extracted nucleic acid into a PCR eight-link EP tube placed in an ice box;
finally, all the wastes are automatically collected into an external garbage can, so that the full-automatic nucleic acid extraction in the whole process is completed.
In summary, the present invention is not limited to the preferred embodiments, but is intended to cover modifications and equivalent arrangements included within the scope of the appended claims and their equivalents.
Claims (10)
1. A full-automatic nucleic acid extraction appearance, its characterized in that: comprises a platform assembly (2) arranged on a bottom plate (1), a nucleic acid extraction assembly (3) and an automatic pipetting assembly (4), wherein the platform assembly is used for placing various reagents and containers, the positions of the reagents and the containers on the bottom plate (1) can be freely combined, the nucleic acid extraction assembly (3) is used for magnetic nucleic acid extraction, and the automatic pipetting assembly (4) is used for precise liquid transfer between the platform assembly (2) and the nucleic acid extraction assembly (3);
the nucleic acid extraction component (3) comprises a 96-deep well plate (301) arranged on the bottom plate (1);
the bottom plate (1) is also provided with a baffle device (5), the baffle device (5) comprises a first baffle (501) arranged above the 96 deep hole plate (301), one end of the first baffle (501) is provided with a first rotating shaft (503) and a second rotating shaft (504) which are arranged in parallel, and the distance between the first rotating shaft (503) and the edge of the first baffle (501) is smaller than the distance between the second rotating shaft (504) and the edge of the first baffle (501); one end of the first rotating shaft (503) and one end of the second rotating shaft (504) are connected with a driving mechanism (502) for driving the baffle I (501) to turn over;
the driving mechanism (502) comprises a baffle shell (505), the first rotating shaft (503) penetrates through the baffle shell (505) and is connected with a third rotating shaft (507) through a first connecting rod (506), and the second rotating shaft (504) penetrates through the baffle shell (505) and is connected with a transmission assembly (509) capable of driving the second rotating shaft (504) to do circular arc motion through a second connecting rod (508); the input of drive assembly (509) is equipped with step motor (510), offer on baffle shell (505) confession first motion groove (511) of first axis of rotation (503) motion, the below in first motion groove (511) is equipped with confession second motion groove (512) of second axis of rotation (504) motion, the radian in second motion groove (512) is greater than first motion groove (511), works as second axis of rotation (504) motion extremely the highest department in second motion groove (512) the height of first axis of rotation (503) with second axis of rotation (504) is the same.
2. The fully automated nucleic acid extractor of claim 1, wherein: the platform assembly (2) comprises a reagent tank carrier (201), an EP pipe carrier (202), a blood collection pipe carrier (203), a PCR eight-connection EP pipe carrier (204) and a TIP head carrier (205) which are arranged on the bottom plate (1), a reagent tank (206) is arranged on the reagent tank carrier (201), an EP pipe rack (207) is arranged on the EP pipe carrier (202), a blood collection pipe (208) is arranged on the blood collection pipe carrier (203), a PCR eight-connection EP pipe (209) is arranged on the PCR eight-connection EP pipe carrier (204), and a TIP head frame (210) is arranged on the TIP head carrier (205).
3. The fully automated nucleic acid extractor of claim 1, wherein: the nucleic acid extraction component (3) further comprises a first X-axis movement mechanism (303) arranged above the 96-deep well plate (301), a first Z-axis movement mechanism (304) arranged on the first X-axis movement mechanism (303) and a nucleic acid extraction arm (302) arranged on the first Z-axis movement mechanism (304); a plurality of magnetic sleeves (306) are arranged on the nucleic acid extraction arm (302) through a first connecting plate (305), and a plurality of magnetic sleeves (307) which are vertically arranged and can extend into the 96 deep hole plate (301) are arranged on the magnetic sleeves (306); the nucleic acid extraction arm (302) is located above the magnetic sleeve frame (306) and is provided with a plurality of magnetic rod frames (309) through a second connecting plate (308), and the magnetic rod frames (309) are provided with a plurality of magnetic rods (310) which can extend into the magnetic sleeve (307).
4. The fully automatic nucleic acid extractor of claim 3, wherein: the driving mechanism (502) can be turned over to be right above the 96-deep hole plate (301).
5. The fully automatic nucleic acid extractor of claim 2, wherein: a baffle circuit board (513) electrically connected with the stepping motor (510) is further arranged in the baffle shell (505).
6. The fully automated nucleic acid extractor of claim 1, wherein: the automatic pipetting component (4) comprises a second X-axis movement mechanism (402) arranged above the nucleic acid extraction component (3) and a first Y-axis movement mechanism (403) arranged on the second X-axis movement mechanism (402), a pipetting arm (401) is arranged on the first Y-axis movement mechanism (403), a plurality of pipetting units are arranged on the pipetting arm (401), and each pipetting unit comprises a second Y-axis movement mechanism (404), a second Z-axis movement mechanism (405) arranged on the second Y-axis movement mechanism (404) and a sampling tube (406) arranged on the second Z-axis movement mechanism (405).
7. The fully automatic nucleic acid extractor of claim 6, wherein: a waste collection device (6) is further arranged on the bottom plate (1), and the waste collection device (6) comprises a TIP head unloading device (601) for assisting the pipetting arm (401) to remove the waste TIP heads from the sampling tube (406); a waste slideway (602) is arranged under the TIP head unloading device (601), and a containing device for the waste TIP heads is arranged at the bottom of the waste slideway (602).
8. The fully automatic nucleic acid extractor of claim 3, wherein: the heating device is characterized in that a plurality of heating assemblies (7) are arranged below the 96 deep hole plates (301), each heating assembly (7) comprises a heating block (701), a heating resistor (702) is arranged below each heating block (701), and a heat insulation sleeve (703) is arranged between each heating resistor (702) and the bottom plate (1).
9. The fully automatic nucleic acid extractor of claim 2, wherein: be equipped with a plurality of parallel arrangement's guided way (8) on EP pipe carrier (202), the bottom of EP pipe support (207) be equipped with guided way (8) complex draw-in groove (9), EP pipe support (207) are made for the polyoxymethylene material.
10. The fully automatic nucleic acid extractor of claim 2, wherein: the periphery of the TIP head carrier (205) is coated with a second baffle (10), and a plurality of elastic sheets (11) for clamping the TIP head frame (210) on the TIP head carrier (205) are arranged on the top surface of the TIP head carrier (205).
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| CN108048302B true CN108048302B (en) | 2024-03-19 |
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