CN107287092A - Nucleic acid extraction instrument - Google Patents
Nucleic acid extraction instrument Download PDFInfo
- Publication number
- CN107287092A CN107287092A CN201710702031.5A CN201710702031A CN107287092A CN 107287092 A CN107287092 A CN 107287092A CN 201710702031 A CN201710702031 A CN 201710702031A CN 107287092 A CN107287092 A CN 107287092A
- Authority
- CN
- China
- Prior art keywords
- unit
- nucleic acid
- area
- acid extraction
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of nucleic acid extraction instrument, including workbench, sample unit on workbench, reagent unit, extraction unit, pipette tips unit, waste unit, liquid sucking device and controller, the workbench includes first area, the second area being oppositely arranged with first area and the 3rd region between first area and second area, pipette tips unit and sample unit are respectively arranged in first area and the 3rd region, waste unit, extraction unit and reagent unit are arranged in second area, and extraction unit is located between waste unit and reagent unit, liquid sucking device is controlled in a first direction in first area according to controller, move back and forth between second area and the 3rd region, and liquid sucking device also in the second direction intersected with first direction in waste unit, move back and forth between extraction unit and reagent unit.Above-mentioned nucleic acid extraction instrument can carry out automatic nucleic acid extraction, more efficient, and whole equipment structure more compact.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, more particularly to nucleic acid extraction instrument.
Background technology
In recent years, with the high speed development of molecular biology, large quantities of molecular biosciences based on nucleic acid have been emerged
Experiment, such as sequencing of two generations, quantitative fluorescent PCR, genetic chip, making nucleic acid molecular hybridization are learned, and DNA integrality, RNA degrade
Degree, the purity of nucleic acid and concentration etc. all produce more or less influence to molecular biology experiment.In addition precisely medical treatment is included
Inside, the especially emergency treatment of the medical applications based on nucleic acid is just being obtained more and more using (such as cause of disease quick diagnosis technology)
Pay attention to and apply, strong influence traditional medical diagnostic method, improve quick diagnosis level.
Method for extracting nucleic acid main at present is paramagnetic particle method, foundation and pellosil centrifugal column identical principle, with nanometer
Technology the surface of superparamagnetic nano particle is improved and surface modification after, be prepared into superparamagnetism silica nano magnetic
Pearl, the magnetic bead can specifically be recognized with nucleic acid molecules and efficiently combined on micro interface.Utilize silica nanoparticle
Superparamagnetism, can be from blood, animal tissue, food, cause of disease in the presence of guanidine hydrochloride, guanidinium isothiocyanate etc. and externally-applied magnetic field
Nucleic acid is isolated in microorganism equal samples.
Paramagnetic particle method nucleic acid extraction can be generally divided into cracking, with reference to, washing and elute, however, by manually carrying out
Need to expend long time, influence the efficiency of nucleic acid extraction, and nucleic acid easily degrades, extraction time is long to be influenceed to be extracted
The quality of nucleic acid.
The content of the invention
Based on this, it is necessary to long for the above-mentioned manually progress paramagnetic particle method nucleic acid extraction consuming time, nucleic acid extraction effect
There is provided a kind of simple efficient nucleic acid extraction instrument for the problem of rate is low.
A kind of nucleic acid extraction instrument, including workbench, the sample unit on the workbench, reagent unit, carry
Unit, pipette tips unit, waste unit, liquid sucking device and controller are taken, the workbench includes first area and firstth area
Domain is with respect to second area and the 3rd region being arranged between the first area and the second area, the pipette tips unit
It is respectively arranged on the sample unit in the first area and the 3rd region, the waste unit, the extraction unit
And the reagent unit is arranged in the second area, and the extraction unit is located at the waste unit and the reagent list
Between member, the liquid sucking device according to the controller control in a first direction in the first area, second area and
Move back and forth between 3rd region, and the liquid sucking device gives up also in the second direction intersected with the first direction in described
Move back and forth between liquid unit, the extraction unit and the reagent unit.
Above-mentioned nucleic acid extraction instrument is used to extract nucleic acid, and liquid sucking device is firstly the need of the pipette tips unit in mobile first area
Place's assembling pipette tips, are then moved along the first direction at the sample unit in second area, pass through pipette tips pipette samples to extraction
Unit, the nucleic acid in sample, liquid sucking device edge after extraction unit extracts end nucleic acid are extracted in extraction unit by paramagnetic particle method
Waste liquid is moved to waste unit, the extraction of the completion nucleic acid of automation, it is not necessary to artificial operation, the core of extraction by second direction movement
Sour better quality, it is more efficient.Also, workbench is divided into multiple regions side by side, each functional area modularization makes
Workbench is compacter, reduces the overall size of whole workbench.Meanwhile, extraction unit is located at the 3rd region, and sample unit
In the second area and first area that extraction unit both sides are respectively arranged on pipette tips unit, sample unit and pipette tips unit are mutually remote
From preventing that the pipette tips placed in multiple samples and pipette tips unit in sample unit from intersecting pollution, it is to avoid pipette tips are by sample
Sample pollution in unit, improves nucleic acid extraction precision.
In one of the embodiments, the controller is additionally operable to control the liquid sucking device absorption liquid to leave liquid table
Secondary returning suction is carried out behind face.
In one of the embodiments, the extraction unit includes deep-well plates, carrier and the magnetic machine for being placed with reaction tube
Structure, the deep-well plates are detachably arranged on the carrier, the magnetic mechanism in the carrier and including displacement piece and
The bar magnet being connected with the displacement piece, the displacement piece is used to drive the bar magnet to closer or far from the reaction pipe outer wall
Move in direction.
In one of the embodiments, the workbench also includes being located between the first area and the 3rd region
The 4th region, the nucleic acid extraction instrument also include grabbing device, be arranged in the 4th region heating unit, vibration
Unit and temperature reducing unit, the grabbing device are used for deep-well plates described in pick-and-place, and the grabbing device can be along the first direction
Move back and forth between the 3rd region and the 4th region, the grabbing device can also be along the second direction described
Move back and forth between heating unit, the oscillating unit and the temperature reducing unit.
In one of the embodiments, in addition to the side wall located at the workbench side, the side wall is along described first
Direction extends, and the liquid sucking device and the grabbing device are movably arranged on the side wall, and respectively close to described
Sample unit and the pipette tips unit are set.
In one of the embodiments, the liquid sucking device and the grabbing device include moving guide rail and executive item,
The moving guide rail is movably arranged on the side wall and drives the executive item to be moved back and forth along the first direction, and
The moving guide rail extends along the second direction, and the executive item is movably arranged on the moving guide rail.
In one of the embodiments, the controller be additionally operable to control the moving guide rail in the liquid sucking device and
The executive item is moved simultaneously along the first direction and the second direction respectively；And/or
The controller is additionally operable to control the moving track and the executive item in the grabbing device respectively along institute
State first direction and the second direction simultaneously mobile.
In one of the embodiments, in addition to positioned at the pipette tips unit side and in the 3rd region
PCR construction units, the liquid sucking device can be moved between the extraction unit and the PCR construction units.
In one of the embodiments, in addition to barcode scanner, the barcode scanner on the workbench and
Positioned at side of the sample unit away from the extraction unit.
In one of the embodiments, the garbage area includes pipette tips garbage area and waste.
Brief description of the drawings
Fig. 1 is the structural representation at one visual angle of one embodiment of the invention amplifying nucleic acid extraction equipment；
Fig. 2 is the structural representation of another angle of nucleic acid extraction instrument shown in Fig. 1；
Fig. 3 is the structural representation of extraction unit in nucleic acid extraction instrument shown in Fig. 1.
Embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give the preferred embodiment of the present invention.But, the present invention can be realized in many different forms, however it is not limited to herein
Described embodiment.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more saturating
It is thorough comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or can also have element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " level ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention
The implication that technical staff is generally understood that is identical.Term used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein " and/or " include one or more phases
The arbitrary and all combination of the Listed Items of pass.
As Figure 1-3, one embodiment of the invention amplifying nucleic acid extraction equipment 100 includes workbench 10, is located at workbench
Sample unit 20, reagent unit 30, extraction unit 40, pipette tips unit 50, waste unit 60, liquid sucking device 70 and control on 10
Device (not shown), workbench 10 includes first area 12, the second area 14 relative with first area 12 and located at the firstth area
The 3rd region 16 between domain 12 and second area 14, pipette tips unit 50 and sample unit 20 are respectively arranged on first area 12 and
In three regions 16, waste unit 60, extraction unit 40 and reagent unit 30 are arranged in second area 14, and extraction unit 40 is set
Between waste unit 60 and reagent unit 30, liquid sucking device 70 is according to the control of controller in a first direction in first area
12nd, move back and forth between the region 16 of second area 14 and the 3rd, and liquid sucking device 70 also resides in intersecting second of first direction
Move back and forth on direction between waste unit 60, extraction unit 40 and reagent unit 30.
It is to be appreciated that first direction is the longitudinally of workbench in Fig. 1, second direction is the width side of workbench
To.
Above-mentioned nucleic acid extraction instrument 100 is used to extract nucleic acid, and liquid sucking device 70 is firstly the need of in mobile first area 12
Pipette tips are assembled at pipette tips unit 50, then moves along the first direction at the sample unit 20 in second area 14, passes through pipette tips
Pipette samples extract the nucleic acid in sample in extraction unit 40 to extraction unit 40 by paramagnetic particle method, and liquid sucking device 70 is being carried
The unit 40 is taken to extract to move in a second direction after end nucleic acid waste liquid moved into waste unit 60, the completion nucleic acid of automation is carried
Take, it is not necessary to which artificial operation, the Nucleic acid quality of extraction is more preferably, more efficient.Also, by workbench 10 be divided into it is multiple side by side
Region, by each functional area modularization, makes workbench 10 compacter, reduces the overall size of whole workbench 10.Meanwhile,
Extraction unit 40 is located at the 3rd region 16, and sample unit 20 and pipette tips unit 50 are respectively arranged on the second of the both sides of extraction unit 40
In region 14 and first area 12, sample unit 20 and pipette tips unit 50 are located remotely from each other, and prevent multiple samples in sample unit 20
The pipette tips placed in product and pipette tips unit 50 intersect pollution, it is to avoid pipette tips are polluted by the sample in sample unit 20, improve
Nucleic acid extraction precision.
In one embodiment, controller is additionally operable to control liquid sucking device 70 and draws liquid to leave and carry out two after liquid surface
Secondary resorption, prevents pipette tips from hanging sample in pollution workbench 10 and sample unit 10 etc. after drop, further improves nucleic acid extraction
Precision, realizes the nucleic acid extraction of high-precision automatic.
In one embodiment, extraction unit 40 includes deep-well plates 41, carrier 42 and the magnetic machine for being placed with reaction tube 412
Structure 43, deep-well plates 41 are detachably arranged on carrier 42, magnetic mechanism 43 in the carrier 42 and including displacement piece 432 and with position
The bar magnet 434 that part 432 is connected is moved, displacement piece 432 is used to drive bar magnet 434 to the direction closer or far from the outer wall of reaction tube 412
It is mobile.When extracting nucleic acid, sample, lysate, suspension containing magnetic beads etc. are put into reaction tube 412 and carry out action breaks, after reaction
Can obtain nucleic acid and magnetic bead, bar magnet 434 can in the presence of displacement piece 432 close to reaction tube 412 outer wall, enrichment with magnetic bead is existed
The bottom of reagent, then shifts nucleic acid, completes the extraction of nucleic acid.
Further, extraction unit 40 also includes vibration main frame 45, and vibration main frame 45 includes actuator 452 and eccentric part
454, carrier 42 is on eccentric part 454, and the driving eccentric part 454 of actuator 452 produces eccentric motion, and then drives carrier 42 to shake
Swing, available for the liquid mixed in reaction tube 412.
In one embodiment, nucleic acid extraction instrument 100 is also included positioned at the side of pipette tips unit 50 and located at first area
PCR construction units 80 in 12, liquid sucking device 70 can be moved between extraction unit 40 and PCR construction units 80, so can be with
The nucleic acid extracted is moved to PCR construction units 80 by liquid sucking device 70, in case copy detection of next step etc..
In one embodiment, nucleic acid extraction instrument 100 also includes barcode scanner 90, and barcode scanner 90 is located at work
On platform 10 and positioned at side of the sample unit 20 away from extraction unit 40, sample unit 20 is put into by the record of barcode scanner 90
Each sample information, be easy to keep track of the information of each sample.
In one embodiment, workbench 10 also includes the 4th region being located between the region 16 of first area 12 and the 3rd
18, nucleic acid extraction instrument also includes grabbing device 91, the heating unit 92 being arranged in the 4th region 18, oscillating unit 93 and drop
Warm unit 94, grabbing device 91 is used for pick-and-place deep-well plates 41, and grabbing device 91 can be in the first direction in the 3rd region 16 and the
Move back and forth between four regions 18, grabbing device 91 can also be single in oscillating unit 93, heating unit 92 and cooling in a second direction
Move back and forth between member 94.
During nucleic acid is extracted, the deep-well plates 41 in extraction unit 40 can be captured by grabbing device 91 and are moved
It is dynamic, added in deep-well plates 41 and the acceleration cracking of heating unit 92 is can be moved to after cracked solution, can also be by grabbing after the completion of cracking
Take device 91 to capture deep-well plates 41 and be moved to temperature reducing unit 94, accelerate the sample cooling after cracking, deep-well plates 41 can be also crawled
The crawl of device 91 is moved to oscillating unit 93, and oscillating unit 93 can vibrate the solution mixed in deep-well plates 41, thus by vibration
Unit 93, heating unit 92 and temperature reducing unit 94 accelerate the process of nucleic acid extraction, and by heating unit 92, oscillating unit 93 and
Temperature reducing unit 94 is together in the 4th region 18, and the related unit of function is positioned in the same area, makes whole workbench 10
On unit it is compacter, make nucleic acid extraction instrument 100 is overall more to minimize.
In one embodiment, nucleic acid extraction instrument 100 also includes the side wall 11 located at the side of workbench 10, the edge of side wall 11
First direction extends, and liquid sucking device 70 and grabbing device 91 are movably arranged on side wall 11, and respectively close to sample list
Member 20 and pipette tips unit 50 are set, and so liquid sucking device 70 and crawl assembling device 91 are movably arranged on side wall 11, can
Moved with the absorption that sample is realized by the movement along side wall 11, and liquid sucking device 70 sets close to sample unit 20 and is easy to inhale
Sample is taken, and grabbing device 91 is set close to pipette tips unit 50, is easy to the deep-well plates 41 on extraction unit 40 being moved to pipette tips
Heating unit 92, temperature reducing unit 94 and the oscillating unit 93 on the side of unit 50, save the movement of liquid sucking device 70 and grabbing device 91
Time.
Further, liquid sucking device 70 and grabbing device 91 include moving guide rail 72 and executive item 74, moving guide rail 72
It is movably arranged on side wall 11 and drives executive item 74 to move back and forth in the first direction, and moving guide rail 72 is along second party
To extension, executive item 74 is movably arranged on moving guide rail 72, executive item 74 can moving guide rail 72 drive lower edge
First direction is moved, and the second direction that executive item 74 can extend along moving guide rail 72 be moved, and such executive item 74 can be
First direction and second party are moved up, and realize the imbibition of each position and the crawl of deep-well plates 41.
Further, controller is additionally operable to control moving guide rail 72 and executive item 74 in liquid sucking device 70 respectively along the
One direction and second direction are simultaneously mobile, and the motion track of executive item 74 can be oblique line or camber line, will not be broken line, reduce movement
Time；And/or controller is additionally operable to control moving guide rail 72 and executive item 74 in grabbing device 91 to distinguish in the first direction
It is simultaneously mobile with second direction, so selectively control one or both of liquid sucking device 70 and grabbing device 91 edge simultaneously
First direction and second direction movement, save the mobile time.
In one embodiment, garbage area 60 includes pipette tips garbage area 61 and waste 63, during nucleic acid is extracted,
, it is necessary to change used pipette tips after the absorption of a kind of assembling of liquid sucking device 70 sample of pipette tips completion or reagent, the rifle that will be discarded
Head concentrates on pipette tips garbage area 61, and needs removal waste liquid during nucleic acid is extracted, and can pass through the waste liquid of generation
Liquid sucking device 70 is moved to waste 63, and different waste materials is classified, is easy to subsequent treatment.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of nucleic acid extraction instrument, it is characterised in that sample unit, examination including workbench, on the workbench
Agent unit, extraction unit, pipette tips unit, waste unit, liquid sucking device and controller, the workbench include first area, with
The first area is with respect to second area and the 3rd region being arranged between the first area and the second area, institute
State pipette tips unit and the sample unit is respectively arranged in the first area and the 3rd region, the waste unit, institute
State extraction unit and the reagent unit be arranged in the second area, and the extraction unit be located at the waste unit and
Between the reagent unit, the liquid sucking device is controlled in a first direction in the first area, the according to the controller
Move back and forth between two regions and the 3rd region, and the liquid sucking device is also in the second direction intersected with the first direction
On move back and forth between the waste unit, the extraction unit and the reagent unit.
2. nucleic acid extraction instrument according to claim 1, it is characterised in that the controller is additionally operable to control the imbibition
Device draws liquid and leaves progress secondary returning suction after liquid surface.
3. nucleic acid extraction instrument according to claim 1, it is characterised in that the extraction unit includes being placed with reaction tube
Deep-well plates, carrier and magnetic mechanism, the deep-well plates are detachably arranged on the carrier, and the magnetic mechanism is located at described
Bar magnet be connected in carrier and including displacement piece and with the displacement piece, the displacement piece for driving the bar magnet to close or
Direction movement away from the reaction pipe outer wall.
4. nucleic acid extraction instrument according to claim 3, it is characterised in that the workbench also includes being located at described first
The 4th region between region and the 3rd region, the nucleic acid extraction instrument also includes grabbing device, is arranged at described the
Heating unit, oscillating unit and temperature reducing unit in four regions, the grabbing device are used for deep-well plates described in pick-and-place, and described grab
Device is taken to move back and forth along the first direction between the 3rd region and the 4th region, the grabbing device is also
It can move back and forth along the second direction between the heating unit, the oscillating unit and the temperature reducing unit.
5. nucleic acid extraction instrument according to claim 4, it is characterised in that also including the side located at the workbench side
Wall, the side wall extends along the first direction, and the liquid sucking device and the grabbing device are movably arranged at described
On the wall of side, and set respectively close to the sample unit and the pipette tips unit.
6. nucleic acid extraction instrument according to claim 5, it is characterised in that the liquid sucking device and the grabbing device are equal
Including moving guide rail and executive item, the moving guide rail is movably arranged on the side wall and drives the executive item along institute
First direction is stated to move back and forth, and the moving guide rail extends along the second direction, the executive item is movably arranged at
On the moving guide rail.
7. nucleic acid extraction instrument according to claim 6, it is characterised in that the controller is additionally operable to control the imbibition
The moving guide rail and the executive item in device are moved simultaneously along the first direction and the second direction respectively；With/
Or
The controller is additionally operable to control the moving track and the executive item in the grabbing device respectively along described
One direction and the second direction are simultaneously mobile.
8. nucleic acid extraction instrument according to claim 1, it is characterised in that also including positioned at the pipette tips unit side simultaneously
PCR construction units in the 3rd region, the liquid sucking device can be in the extraction unit and the PCR construction units
Between move.
9. nucleic acid extraction instrument according to claim 1, it is characterised in that also including barcode scanner, the bar code is swept
Device is retouched on the workbench and positioned at side of the sample unit away from the extraction unit.
10. nucleic acid extraction instrument according to claim 1, it is characterised in that the garbage area include pipette tips garbage area and
Waste.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710702031.5A CN107287092A (en) | 2017-08-16 | 2017-08-16 | Nucleic acid extraction instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710702031.5A CN107287092A (en) | 2017-08-16 | 2017-08-16 | Nucleic acid extraction instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107287092A true CN107287092A (en) | 2017-10-24 |
Family
ID=60105994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710702031.5A Pending CN107287092A (en) | 2017-08-16 | 2017-08-16 | Nucleic acid extraction instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107287092A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760676A (en) * | 2017-11-14 | 2018-03-06 | 孔治 | A kind of full-automatic extraction apparatus of paramagnetic particle |
| CN108048302A (en) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | Full automatic instrument for extracting nucleic acid |
| CN110305935A (en) * | 2019-08-07 | 2019-10-08 | 湖南圣湘生物科技有限公司 | The full-automatic method for extracting nucleic acid of Septin9 gene methylation |
| CN110387322A (en) * | 2018-04-09 | 2019-10-29 | 恺硕生物科技(厦门)有限公司 | Automatic nucleic acid extracts detection system |
| CN111172008A (en) * | 2018-11-09 | 2020-05-19 | 开启基因股份有限公司 | Method and device for automatic nucleic acid extraction |
| CN111218386A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Silica bead method nucleic acid extraction equipment and use method and application thereof |
| CN112210476A (en) * | 2020-11-12 | 2021-01-12 | 药明激创(佛山)生物科技有限公司 | Be applied to biological liquid workstation |
| CN113106022A (en) * | 2021-04-09 | 2021-07-13 | 天津市第三中心医院 | Full-automatic multifunctional cell processing system |
| CN113684120A (en) * | 2021-08-20 | 2021-11-23 | 圣湘生物科技股份有限公司 | Nucleic acid detection apparatus and nucleic acid detection method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090130745A1 (en) * | 2007-07-13 | 2009-05-21 | Handylab, Inc. | Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN106367311A (en) * | 2016-12-08 | 2017-02-01 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction system and using method thereof |
| CN106520540A (en) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | Integrated full-automatic nucleic acid detection reaction system |
| CN206204280U (en) * | 2016-11-22 | 2017-05-31 | 安图实验仪器(郑州)有限公司 | It is suitable to the single nucleic acid extraction box of diagnostic nucleic acid system |
-
2017
- 2017-08-16 CN CN201710702031.5A patent/CN107287092A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090130745A1 (en) * | 2007-07-13 | 2009-05-21 | Handylab, Inc. | Integrated Apparatus for Performing Nucleic Acid Extraction and Diagnostic Testing on Multiple Biological Samples |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN105543089A (en) * | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN206204280U (en) * | 2016-11-22 | 2017-05-31 | 安图实验仪器(郑州)有限公司 | It is suitable to the single nucleic acid extraction box of diagnostic nucleic acid system |
| CN106367311A (en) * | 2016-12-08 | 2017-02-01 | 湖南圣湘生物科技有限公司 | Nucleic acid extraction system and using method thereof |
| CN106520540A (en) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | Integrated full-automatic nucleic acid detection reaction system |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760676A (en) * | 2017-11-14 | 2018-03-06 | 孔治 | A kind of full-automatic extraction apparatus of paramagnetic particle |
| CN107760676B (en) * | 2017-11-14 | 2020-06-23 | 孔治 | Full-automatic extraction instrument for paramagnetic particles |
| CN108048302A (en) * | 2018-02-01 | 2018-05-18 | 北京同立创辉仪器有限公司 | Full automatic instrument for extracting nucleic acid |
| CN108048302B (en) * | 2018-02-01 | 2024-03-19 | 北京同立创辉仪器有限公司 | Full-automatic nucleic acid extractor |
| CN110387322A (en) * | 2018-04-09 | 2019-10-29 | 恺硕生物科技(厦门)有限公司 | Automatic nucleic acid extracts detection system |
| CN111172008A (en) * | 2018-11-09 | 2020-05-19 | 开启基因股份有限公司 | Method and device for automatic nucleic acid extraction |
| CN111172008B (en) * | 2018-11-09 | 2021-09-28 | 开启基因股份有限公司 | Method and device for automatic nucleic acid extraction |
| CN110305935A (en) * | 2019-08-07 | 2019-10-08 | 湖南圣湘生物科技有限公司 | The full-automatic method for extracting nucleic acid of Septin9 gene methylation |
| CN111218386A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Silica bead method nucleic acid extraction equipment and use method and application thereof |
| CN112210476A (en) * | 2020-11-12 | 2021-01-12 | 药明激创(佛山)生物科技有限公司 | Be applied to biological liquid workstation |
| CN113106022A (en) * | 2021-04-09 | 2021-07-13 | 天津市第三中心医院 | Full-automatic multifunctional cell processing system |
| CN113684120A (en) * | 2021-08-20 | 2021-11-23 | 圣湘生物科技股份有限公司 | Nucleic acid detection apparatus and nucleic acid detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107287092A (en) | Nucleic acid extraction instrument | |
| US10351894B2 (en) | Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices | |
| CN102764607B (en) | A device and method for processing and mixing magnetic particles in a liquid medium | |
| US8263387B2 (en) | Sheath flow devices and methods | |
| CN106367311B (en) | A kind of nucleic acid extraction system and its application method | |
| US7955836B2 (en) | Microfluidic mixing and analytical apparatus | |
| US20180266951A1 (en) | Flow cells utilizing surface-attached structures, and related systems and methods | |
| CN207468608U (en) | Nucleic acid extraction instrument | |
| EP3523003B1 (en) | Particle isolation/enrichment using continuous closed-loop micro-fluidics | |
| DE102008035771B4 (en) | Apparatus and method for the automatic detection of biological particles | |
| CN104736718A (en) | Devices and methods for manipulating components in a fluid sample | |
| CN108048302A (en) | Full automatic instrument for extracting nucleic acid | |
| EP1896858A1 (en) | Device and method for preparing a sample for an analysis and device and method for analyzing a sample | |
| Surendran et al. | Microfluidic devices for magnetic separation of biological particles: a review | |
| JP2008544277A (en) | Device for moving magnetic particles | |
| CN111182970B (en) | Method for treating biological samples with magnetic particles | |
| CN207468609U (en) | Nucleic acid extraction instrument | |
| US20180229237A1 (en) | Flow cells utilizing surface-attached structures, and related systems and methods | |
| CN105424952B (en) | Microfluidic mixing device and method thereof | |
| CN207786635U (en) | Imbibition pipette tips carrier and nucleic acid extraction instrument | |
| JP2014093988A (en) | Method of manipulating solid carriers and apparatus of manipulating solid carriers | |
| EP3793716A1 (en) | Systems and methods for nucleic acid purification using flow cells with actuated surface-attached structures | |
| Kanies et al. | A modular microfluidic device that uses magnetically actuatable microposts for enhanced magnetic bead-based workflows | |
| JP4269002B1 (en) | Reaction apparatus and method | |
| JP4269001B1 (en) | Reaction apparatus and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan Applicant after: Shengxiang Biotechnology Co., Ltd Address before: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan Applicant before: Sansure Biotech Inc. |
|
| CB02 | Change of applicant information |