Utility model content
Based on this, it is necessary to for the problem of Aerosol Pollution, providing a kind of nucleic acid extraction instrument in above-mentioned nucleic acid extraction.
A kind of nucleic acid extraction instrument, including workbench, support element, exhaust apparatus, nucleic acid extraction module and waste unit, institute
Support element is stated set on the workbench side and working space, the nucleic acid extraction module and institute are formed between the workbench
Waste unit is stated on the workbench and in the working space, and the waste unit is set close to the support element
It puts, the waste unit is corresponded on the support element, the exhaust apparatus is set.
In above-mentioned nucleic acid extraction instrument, nucleic acid extraction module, which is set on workbench, to be used to the nucleic acid in sample passing through magnetic bead
Method extracts, and support element is set on workbench side and working space is formed between workbench, and waste unit is close
Support element is set on workbench, when nucleic extraction module carries out nucleic acid extraction and can when waste unit launches discarded liquid
Aerosol can be generated, and aerosol can be likely to sample, reagent and product of extraction etc. to generate pollution, by being set on
The aerosol side suction generated in working space can be gone out working space, and exhaust apparatus is set by the exhaust apparatus on support element
In the side of working space with preferable exhaust effect, the aerosol side suction that will effectively can be spread around, so
Prevent the pollution of aerosol in nucleic acid extraction.
The nucleic acid extraction module includes reagent unit and extraction unit, the workbench in one of the embodiments,
Including first area, the reagent unit, the extraction unit and the waste unit are set in the first area, and institute
Extraction unit is stated between the reagent unit and the waste unit, the exhaust apparatus is set to the support element and institute
State first area corresponding position.
Controller and liquid absorption device are further included in one of the embodiments, and the nucleic acid extraction module further includes sample
Unit and pipette tips unit, the workbench further include the second area being oppositely arranged and third region, and the first area is set on
Between the second area and the third region, the pipette tips unit and the sample unit are respectively arranged on the second area
In the third region, the liquid absorption device is controlled in a first direction according to the controller in the first area, the
Move back and forth between two regions and third region, and the liquid absorption device is also in the second direction intersected with the first direction
On move back and forth between the waste unit, the extraction unit and the reagent unit.
The controller is additionally operable to that the liquid absorption device absorption liquid is controlled to leave liquid table in one of the embodiments,
Secondary returning suction is carried out behind face.
The extraction unit includes the deep-well plates, carrier and the magnetic machine that are placed with reaction tube in one of the embodiments,
Structure, the deep-well plates are detachably arranged on the carrier, the magnetic mechanism be set in the carrier and including displacement piece and
The bar magnet being connect with the displacement piece, the displacement piece are used to drive the bar magnet to closer or far from the reaction pipe outer wall
It moves in direction.
The workbench is further included between the first area and the second area in one of the embodiments,
The fourth region, the nucleic acid extraction instrument further include grabbing device, the heating unit being set in the fourth region, oscillation
Unit and temperature reducing unit, the grabbing device is used for deep-well plates described in pick-and-place, and the grabbing device can be along the first direction
Move back and forth between the first area and the fourth region, the grabbing device can also be along the second direction described
Move back and forth between heating unit, the oscillating unit and the temperature reducing unit.
The support element extends along the first direction in one of the embodiments, the liquid absorption device and described grabs
Device is taken to be movably arranged on the support element, and is set respectively close to the sample unit and the pipette tips unit.
The liquid absorption device and the grabbing device include moving guide rail and executive item in one of the embodiments,
The moving guide rail is movably arranged on the support element and the executive item is driven to be moved back and forth along the first direction,
And the moving guide rail extends in a second direction, the executive item is movably arranged on the moving guide rail.
It further includes in one of the embodiments, positioned at the pipette tips unit side and in the third region
PCR construction units, the liquid absorption device can move between the extraction unit and the PCR construction units.
Further include barcode scanner in one of the embodiments, the barcode scanner be set on the workbench and
Positioned at side of the sample unit far from the extraction unit;And the garbage area includes pipette tips garbage area and waste.
Specific embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms
It realizes, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments makes to the utility model
The understanding of disclosure more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to the technology of the utility model
The normally understood meaning of technical staff in domain is identical.It is only in the term used in the description of the utility model herein
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein " and/or " include
The arbitrary and all combination of one or more relevant Listed Items.
As shown in Figs. 1-3, one embodiment amplifying nucleic acid extraction equipment 100 of the utility model include workbench 10, support element 20,
Exhaust apparatus 30, nucleic acid extraction module 40 and waste unit 50, support element 20 be set on 10 side of workbench and with workbench 10 it
Between form working space 11, nucleic acid extraction module 40 and waste unit 50 are set on workbench 10 and in working space 11,
And waste unit 50 is set close to support element 20, and waste unit 50 is corresponded on support element 20 and sets exhaust apparatus 30.
In above-mentioned nucleic acid extraction instrument 100, nucleic acid extraction module 40 is set on workbench 10 and is used for the nucleic acid in sample
It is extracted by paramagnetic particle method, and support element 20 is set on 10 side of workbench and forms working space between workbench 10
11, and waste unit 50 close to support element 20 be set on workbench 10 on, when nucleic extraction module 40 carry out nucleic acid extraction and
Waste unit 50 may generate aerosol when launching discarded liquid, and aerosol can be to sample, reagent and the production of extraction
Object etc. is likely to generate pollution, the gas that can will be generated in working space 11 by the exhaust apparatus 30 being set on support element 20
The suction of colloidal sol side goes out working space 11, and the side that exhaust apparatus 30 is set on working space 11 has preferable exhaust effect,
The aerosol side suction that will effectively can be spread around, so prevents the pollution of aerosol in nucleic acid extraction.
Optionally, filter cotton is provided in exhaust apparatus 30, the impurity in air filtering can be crossed, improved from working space 11
The quality of the air of discharge.
In one embodiment, nucleic acid extraction module 40 includes reagent unit 41 and extraction unit 42, and workbench 10 includes
First area 12, reagent unit 41, extraction unit 42 and waste unit 50 are set in first area 12, and are carried unit 42 and be located at
Between reagent unit 41 and waste unit 50, exhaust apparatus 30 is set to support element 20 and 12 corresponding position of first area.Row
Device of air 30 corresponds to waste unit 50 and is set on support element 20, and waste unit is set in first area 12, that is to say, that row
The first area 12 that device of air 30 corresponds to workbench 10 is set on support element 20, the extraction unit 42 in first area 12 and useless
Liquid unit 50 is the unit for being easiest to generate aerosol, so close to first area 12, setting can be arranged as early as possible by exhaust apparatus 30
Go out most of aerosol, and reagent unit 41 is set in first area 12 and positioned at the side of extraction unit 42, facilitating will try
Reagent in agent unit 41, which is added in extraction unit 42, carries out nucleic acid extraction.
Specifically, extraction unit 42 includes being placed with deep-well plates 422, carrier 423 and the magnetic mechanism 424 of reaction tube 421,
Deep-well plates 422 are detachably arranged on carrier 423, magnetic mechanism 424 be set on carrier 423 in and including displacement piece 425 and with position
The bar magnet 426 that part 425 connects is moved, displacement piece 425 is used to drive bar magnet 426 to the direction closer or far from 421 outer wall of reaction tube
It is mobile.When extracting nucleic acid, sample, lysate, suspension containing magnetic beads etc. are put into reaction tube 421 and carry out action breaks, after reaction
Nucleic acid and magnetic bead are can obtain, bar magnet 426 can make enrichment with magnetic bead exist under the action of displacement piece 425 close to the outer wall of reaction tube 421
The bottom of reagent, then shifts nucleic acid, completes the extraction of nucleic acid.
Further, extraction unit 42 further includes oscillation host 427, and oscillation host 427 includes actuator 428 and eccentric part
429, carrier 423 is set on eccentric part 429, and actuator 428 drives eccentric part 429 to generate eccentric motion, and then drives carrier 423
Oscillation, available for the liquid in mixing reaction tube 421.
In one embodiment, nucleic acid extraction instrument 100 further includes liquid absorption device 60 and controller (not shown), and nucleic acid carries
Modulus block 40 further includes sample unit 43 and pipette tips unit 44, and workbench 10 further includes the second area 14 being oppositely arranged and third
Region 16, first area 12 are set between second area 14 and third region 16, and pipette tips unit 44 and sample unit 43 are set respectively
In in second area 14 and third unit 16, liquid absorption device 60 according to controller control in a first direction in first area 12,
Move back and forth back and forth between second area 14 and third region 16, and liquid absorption device 60 also reside in that first direction intersects second
Move back and forth between waste unit 50, extraction unit 42 and reagent unit 41 on direction.
It is to be appreciated that first direction is the longitudinally of workbench in Fig. 1, second direction is the width side of workbench
To.Then liquid absorption device 60 moves along the first direction firstly the need of pipette tips are assembled at the pipette tips unit 44 in mobile second area 14
It moves at the sample unit 43 in third region 16, by pipette tips pipette samples to extraction unit 42, leads in extraction unit 42
The nucleic acid in paramagnetic particle method extraction sample is crossed, liquid absorption device 60 is mobile in a second direction after extraction unit 42 extracts end nucleic acid will be useless
Liquid moves to waste unit 50, and the extraction of the completion nucleic acid of automation does not need to manual operation, and the Nucleic acid quality of extraction is more preferable, effect
Rate higher.Also, workbench 10 is divided into multiple regions side by side, by each functional area modularization, makes workbench 10 more
Step up to gather, reduce the whole size of entire workbench 10.Meanwhile extraction unit 42 is set on first area 12, and pipette tips unit 44
It is respectively arranged on sample unit 43 in the second area 14 and third region 16 of 40 both sides of extraction unit, sample unit 43 and pipette tips
Unit 44 is located remotely from each other, and prevents the pipette tips placed in multiple samples and pipette tips unit 44 in sample unit 43 from intersecting dirt
Dye avoids pipette tips from being polluted by the sample in sample unit 43, improves nucleic acid extraction precision.
In one embodiment, the controller in nucleic acid extraction instrument 100 is additionally operable to control liquid absorption device 60 and draws liquid
Secondary returning suction is carried out after leaving liquid level, prevents pipette tips extension from polluting sample in workbench 10 and sample unit 43 etc. after dripping, into one
Step improves the precision of nucleic acid extraction, realizes high-precision automatic nucleic acid extraction.
In one embodiment, nucleic acid extraction instrument 100 is further included positioned at 44 side of pipette tips unit and set on second area
PCR construction units 70 in 14, liquid absorption device 60 can move between extraction unit 42 and PCR construction units 60, so can be with
The nucleic acid extracted is moved to PCR construction units 60 by liquid absorption device 60, in case copy detection of next step etc..
In one embodiment, nucleic acid extraction instrument 100 further includes barcode scanner 80, and barcode scanner 80 is set on work
On platform 10 and positioned at side of the sample unit 43 far from extraction unit 42, sample unit 43 is put by the record of barcode scanner 80
Each sample information, convenient for tracking and recording the information of each sample.
In one embodiment, workbench 10 further includes the fourth region between first area 12 and second area 14
18, nucleic acid extraction instrument further includes grabbing device 90, the heating unit being set in the fourth region 18 92, oscillating unit 93 and drop
Warm unit 94, grabbing device 90 is used for pick-and-place deep-well plates 422, and grabbing device 90 can be along the first direction in first area 12 and the
Move back and forth between four regions 18, grabbing device 90 can also be single in heating unit 92, oscillating unit 93 and cooling in a second direction
Move back and forth between member 94.
During nucleic acid is extracted, the deep-well plates 422 in extraction unit 42 can be captured by grabbing device 90 and are moved
It is dynamic, the acceleration cracking of heating unit 92 is can be moved to after cracked solution is added in deep-well plates 422, it can also be by grabbing after the completion of cracking
Device 90 is taken to capture deep-well plates 422 and is moved to temperature reducing unit 94, accelerates the sample cooling after cracking, deep-well plates 422 can also be grabbed
The crawl of device 90 is taken to be moved to oscillating unit 93, oscillating unit 93 can vibrate the solution in mixing deep-well plates 422, thus by shaking
Swing the process that unit 93, heating unit 92 and temperature reducing unit 94 accelerate nucleic acid extraction.And by heating unit 92, oscillating unit 93
And temperature reducing unit 94 is set in the fourth region 18 together, the relevant unit of function is positioned in the same area, makes entire workbench
Each unit on 10 is compacter, makes nucleic acid extraction instrument 100 is whole more to minimize.
In one embodiment, support element 20 extends in a first direction, and liquid absorption device 60 and grabbing device 90 are removable
Ground is set on support element 20, and is set respectively close to sample unit 43 and pipette tips unit 44, so by liquid absorption device 60 and is grabbed
Assembling device 90 is taken to be movably arranged on support element 20, it can be by realizing that the absorption of sample moves along moving for support element 20
It is dynamic, and liquid absorption device 60 is convenient for pipette samples close to the setting of sample unit 43, and grabbing device 90 is set close to pipette tips unit 44
It puts, convenient for the deep-well plates 422 on extraction unit 42 to be moved to the heating unit 92 on 44 side of pipette tips unit, temperature reducing unit 94 and are shaken
Unit 93 is swung, saves the traveling time of liquid absorption device 60 and grabbing device 90.
Further, liquid absorption device 60 and grabbing device 90 include moving guide rail 61 and executive item 63, moving guide rail 61
It is movably arranged on support element 11 and drives executive item 63 reciprocal along the first direction in first area 12 to third region 16
It is mobile, and moving guide rail 61 extends along the second direction of vertical first direction, and executive item 63 is movably arranged at movement and leads
On rail 61, executive item 63 can move along the first direction under the drive of moving guide rail 61, and executive item 63 can be along moving
The second direction movement that guide rail 61 extends, such executive item 63 can be in a first direction and in the second direction vertical with first direction
It is mobile, realize the imbibition of each position and the crawl of deep-well plates 422.
Further, controller is additionally operable to moving guide rail 61 in control liquid absorption device 60 and executive item 63 respectively along the
One direction and second direction are mobile simultaneously, and 63 motion track of executive item can be oblique line or camber line, will not be broken line, reduce movement
Time;And/or controller be additionally operable to control grabbing device 90 in moving guide rail 61 and executive item 63 respectively along the first direction
It is mobile simultaneously with second direction, so selectively control one or both of liquid absorption device 60 and grabbing device 90 edge simultaneously
First direction and second direction movement, save the mobile time.
In one embodiment, garbage area 50 includes pipette tips garbage area 52 and waste 54, during nucleic acid is extracted,
After liquid absorption device 50 assembles the absorption that pipette tips complete a kind of sample or reagent, used pipette tips are needed replacing, by discarded rifle
Head concentrates on pipette tips garbage area 52, and removal waste liquid is needed during nucleic acid is extracted, and can pass through the waste liquid of generation
Liquid absorption device 50 is moved to waste 54, different waste materials is classified, convenient for subsequent processing.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, it is all considered to be the range of this specification record.
Embodiment described above only expresses the several embodiments of the utility model, and description is more specific and detailed,
But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that the common skill for this field
For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to
The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.