CN207468609U - Nucleic acid extraction instrument - Google Patents

Nucleic acid extraction instrument Download PDF

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Publication number
CN207468609U
CN207468609U CN201721028999.6U CN201721028999U CN207468609U CN 207468609 U CN207468609 U CN 207468609U CN 201721028999 U CN201721028999 U CN 201721028999U CN 207468609 U CN207468609 U CN 207468609U
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China
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unit
nucleic acid
acid extraction
area
workbench
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CN201721028999.6U
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Chinese (zh)
Inventor
戴立忠
范旭
卓红俞
吴岭
傅国
彭龙
昌雄
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Sanway Gene Tech Inc
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Sansure Biotech Inc
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Abstract

The utility model is related to a kind of nucleic acid extraction instruments, including workbench, support element, exhaust apparatus, nucleic acid extraction module and waste unit, support element is set on workbench side and working space is formed between workbench, nucleic acid extraction module and waste unit are set on workbench and in working space, and waste unit is set close to support element, and waste unit setting exhaust apparatus is corresponded on support element.In above-mentioned nucleic acid extraction instrument, support element is set on workbench side and working space is formed between workbench, and waste unit is set on close to support element on workbench, when nucleic extraction module carries out nucleic acid extraction and may generate aerosol when waste unit launches discarded liquid, the aerosol side suction generated in working space can be gone out working space by being set on the exhaust apparatus on support element, and the side that exhaust apparatus is set on working space has preferable exhaust effect, so prevents the pollution of aerosol in nucleic acid extraction.

Description

Nucleic acid extraction instrument
Technical field
The utility model is related to nucleic acid extraction technical field, more particularly to nucleic acid extraction instrument.
Background technology
In recent years, with the high speed development of molecular biology, large quantities of molecular biosciences based on nucleic acid have been emerged Experiment, such as the sequencing of two generations, quantitative fluorescent PCR, genetic chip, making nucleic acid molecular hybridization are learned, and the integrality of DNA, RNA degrade Degree, the purity of nucleic acid and concentration etc. all generate molecular biology experiment more or less influence.In addition include precisely medical treatment Inside, the especially emergency treatment of the medical applications based on nucleic acid is just obtaining more and more using (such as cause of disease quick diagnosis technology) Pay attention to and it is horizontal to improve quick diagnosis for application, strong influence traditional medical diagnostic method.
Current main method for extracting nucleic acid is paramagnetic particle method, according to the principle identical with pellosil centrifugal column, with nanometer Technology the surface of superparamagnetic nano particle is improved and surface modification after, be prepared into superparamagnetism silica nano magnetic Pearl, the magnetic bead can specifically be identified with nucleic acid molecules and efficiently be combined on micro interface.Utilize silica nanoparticle Superparamagnetism, can be from blood, animal tissue, food, cause of disease under the action of guanidine hydrochloride, guanidinium isothiocyanate etc. and externally-applied magnetic field Nucleic acid is isolated in microorganism equal samples.
Paramagnetic particle method nucleic acid extraction can be generally divided into cracking, combination, washing and elution, and easily be produced in extraction process Angry colloidal sol, aerosol easily pollute sample etc., can generate large effect to last detection of nucleic acids result.
Utility model content
Based on this, it is necessary to for the problem of Aerosol Pollution, providing a kind of nucleic acid extraction instrument in above-mentioned nucleic acid extraction.
A kind of nucleic acid extraction instrument, including workbench, support element, exhaust apparatus, nucleic acid extraction module and waste unit, institute Support element is stated set on the workbench side and working space, the nucleic acid extraction module and institute are formed between the workbench Waste unit is stated on the workbench and in the working space, and the waste unit is set close to the support element It puts, the waste unit is corresponded on the support element, the exhaust apparatus is set.
In above-mentioned nucleic acid extraction instrument, nucleic acid extraction module, which is set on workbench, to be used to the nucleic acid in sample passing through magnetic bead Method extracts, and support element is set on workbench side and working space is formed between workbench, and waste unit is close Support element is set on workbench, when nucleic extraction module carries out nucleic acid extraction and can when waste unit launches discarded liquid Aerosol can be generated, and aerosol can be likely to sample, reagent and product of extraction etc. to generate pollution, by being set on The aerosol side suction generated in working space can be gone out working space, and exhaust apparatus is set by the exhaust apparatus on support element In the side of working space with preferable exhaust effect, the aerosol side suction that will effectively can be spread around, so Prevent the pollution of aerosol in nucleic acid extraction.
The nucleic acid extraction module includes reagent unit and extraction unit, the workbench in one of the embodiments, Including first area, the reagent unit, the extraction unit and the waste unit are set in the first area, and institute Extraction unit is stated between the reagent unit and the waste unit, the exhaust apparatus is set to the support element and institute State first area corresponding position.
Controller and liquid absorption device are further included in one of the embodiments, and the nucleic acid extraction module further includes sample Unit and pipette tips unit, the workbench further include the second area being oppositely arranged and third region, and the first area is set on Between the second area and the third region, the pipette tips unit and the sample unit are respectively arranged on the second area In the third region, the liquid absorption device is controlled in a first direction according to the controller in the first area, the Move back and forth between two regions and third region, and the liquid absorption device is also in the second direction intersected with the first direction On move back and forth between the waste unit, the extraction unit and the reagent unit.
The controller is additionally operable to that the liquid absorption device absorption liquid is controlled to leave liquid table in one of the embodiments, Secondary returning suction is carried out behind face.
The extraction unit includes the deep-well plates, carrier and the magnetic machine that are placed with reaction tube in one of the embodiments, Structure, the deep-well plates are detachably arranged on the carrier, the magnetic mechanism be set in the carrier and including displacement piece and The bar magnet being connect with the displacement piece, the displacement piece are used to drive the bar magnet to closer or far from the reaction pipe outer wall It moves in direction.
The workbench is further included between the first area and the second area in one of the embodiments, The fourth region, the nucleic acid extraction instrument further include grabbing device, the heating unit being set in the fourth region, oscillation Unit and temperature reducing unit, the grabbing device is used for deep-well plates described in pick-and-place, and the grabbing device can be along the first direction Move back and forth between the first area and the fourth region, the grabbing device can also be along the second direction described Move back and forth between heating unit, the oscillating unit and the temperature reducing unit.
The support element extends along the first direction in one of the embodiments, the liquid absorption device and described grabs Device is taken to be movably arranged on the support element, and is set respectively close to the sample unit and the pipette tips unit.
The liquid absorption device and the grabbing device include moving guide rail and executive item in one of the embodiments, The moving guide rail is movably arranged on the support element and the executive item is driven to be moved back and forth along the first direction, And the moving guide rail extends in a second direction, the executive item is movably arranged on the moving guide rail.
It further includes in one of the embodiments, positioned at the pipette tips unit side and in the third region PCR construction units, the liquid absorption device can move between the extraction unit and the PCR construction units.
Further include barcode scanner in one of the embodiments, the barcode scanner be set on the workbench and Positioned at side of the sample unit far from the extraction unit;And the garbage area includes pipette tips garbage area and waste.
Description of the drawings
Fig. 1 is the structure diagram of one embodiment amplifying nucleic acid extraction equipment of the utility model;
Fig. 2 is the structure diagram of another angle of nucleic acid extraction instrument shown in Fig. 1;
Fig. 3 is the structure diagram of extraction unit in nucleic acid extraction instrument shown in Fig. 1.
Specific embodiment
For the ease of understanding the utility model, the utility model is more fully retouched below with reference to relevant drawings It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms It realizes, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments makes to the utility model The understanding of disclosure more thorough and comprehensive.
It should be noted that when element is referred to as " being fixed on " another element, it can be directly on another element Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it can be directly connected to To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ", " right side " and similar statement are for illustrative purposes only.
Unless otherwise defined, all of technologies and scientific terms used here by the article is led with belonging to the technology of the utility model The normally understood meaning of technical staff in domain is identical.It is only in the term used in the description of the utility model herein The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein " and/or " include The arbitrary and all combination of one or more relevant Listed Items.
As shown in Figs. 1-3, one embodiment amplifying nucleic acid extraction equipment 100 of the utility model include workbench 10, support element 20, Exhaust apparatus 30, nucleic acid extraction module 40 and waste unit 50, support element 20 be set on 10 side of workbench and with workbench 10 it Between form working space 11, nucleic acid extraction module 40 and waste unit 50 are set on workbench 10 and in working space 11, And waste unit 50 is set close to support element 20, and waste unit 50 is corresponded on support element 20 and sets exhaust apparatus 30.
In above-mentioned nucleic acid extraction instrument 100, nucleic acid extraction module 40 is set on workbench 10 and is used for the nucleic acid in sample It is extracted by paramagnetic particle method, and support element 20 is set on 10 side of workbench and forms working space between workbench 10 11, and waste unit 50 close to support element 20 be set on workbench 10 on, when nucleic extraction module 40 carry out nucleic acid extraction and Waste unit 50 may generate aerosol when launching discarded liquid, and aerosol can be to sample, reagent and the production of extraction Object etc. is likely to generate pollution, the gas that can will be generated in working space 11 by the exhaust apparatus 30 being set on support element 20 The suction of colloidal sol side goes out working space 11, and the side that exhaust apparatus 30 is set on working space 11 has preferable exhaust effect, The aerosol side suction that will effectively can be spread around, so prevents the pollution of aerosol in nucleic acid extraction.
Optionally, filter cotton is provided in exhaust apparatus 30, the impurity in air filtering can be crossed, improved from working space 11 The quality of the air of discharge.
In one embodiment, nucleic acid extraction module 40 includes reagent unit 41 and extraction unit 42, and workbench 10 includes First area 12, reagent unit 41, extraction unit 42 and waste unit 50 are set in first area 12, and are carried unit 42 and be located at Between reagent unit 41 and waste unit 50, exhaust apparatus 30 is set to support element 20 and 12 corresponding position of first area.Row Device of air 30 corresponds to waste unit 50 and is set on support element 20, and waste unit is set in first area 12, that is to say, that row The first area 12 that device of air 30 corresponds to workbench 10 is set on support element 20, the extraction unit 42 in first area 12 and useless Liquid unit 50 is the unit for being easiest to generate aerosol, so close to first area 12, setting can be arranged as early as possible by exhaust apparatus 30 Go out most of aerosol, and reagent unit 41 is set in first area 12 and positioned at the side of extraction unit 42, facilitating will try Reagent in agent unit 41, which is added in extraction unit 42, carries out nucleic acid extraction.
Specifically, extraction unit 42 includes being placed with deep-well plates 422, carrier 423 and the magnetic mechanism 424 of reaction tube 421, Deep-well plates 422 are detachably arranged on carrier 423, magnetic mechanism 424 be set on carrier 423 in and including displacement piece 425 and with position The bar magnet 426 that part 425 connects is moved, displacement piece 425 is used to drive bar magnet 426 to the direction closer or far from 421 outer wall of reaction tube It is mobile.When extracting nucleic acid, sample, lysate, suspension containing magnetic beads etc. are put into reaction tube 421 and carry out action breaks, after reaction Nucleic acid and magnetic bead are can obtain, bar magnet 426 can make enrichment with magnetic bead exist under the action of displacement piece 425 close to the outer wall of reaction tube 421 The bottom of reagent, then shifts nucleic acid, completes the extraction of nucleic acid.
Further, extraction unit 42 further includes oscillation host 427, and oscillation host 427 includes actuator 428 and eccentric part 429, carrier 423 is set on eccentric part 429, and actuator 428 drives eccentric part 429 to generate eccentric motion, and then drives carrier 423 Oscillation, available for the liquid in mixing reaction tube 421.
In one embodiment, nucleic acid extraction instrument 100 further includes liquid absorption device 60 and controller (not shown), and nucleic acid carries Modulus block 40 further includes sample unit 43 and pipette tips unit 44, and workbench 10 further includes the second area 14 being oppositely arranged and third Region 16, first area 12 are set between second area 14 and third region 16, and pipette tips unit 44 and sample unit 43 are set respectively In in second area 14 and third unit 16, liquid absorption device 60 according to controller control in a first direction in first area 12, Move back and forth back and forth between second area 14 and third region 16, and liquid absorption device 60 also reside in that first direction intersects second Move back and forth between waste unit 50, extraction unit 42 and reagent unit 41 on direction.
It is to be appreciated that first direction is the longitudinally of workbench in Fig. 1, second direction is the width side of workbench To.Then liquid absorption device 60 moves along the first direction firstly the need of pipette tips are assembled at the pipette tips unit 44 in mobile second area 14 It moves at the sample unit 43 in third region 16, by pipette tips pipette samples to extraction unit 42, leads in extraction unit 42 The nucleic acid in paramagnetic particle method extraction sample is crossed, liquid absorption device 60 is mobile in a second direction after extraction unit 42 extracts end nucleic acid will be useless Liquid moves to waste unit 50, and the extraction of the completion nucleic acid of automation does not need to manual operation, and the Nucleic acid quality of extraction is more preferable, effect Rate higher.Also, workbench 10 is divided into multiple regions side by side, by each functional area modularization, makes workbench 10 more Step up to gather, reduce the whole size of entire workbench 10.Meanwhile extraction unit 42 is set on first area 12, and pipette tips unit 44 It is respectively arranged on sample unit 43 in the second area 14 and third region 16 of 40 both sides of extraction unit, sample unit 43 and pipette tips Unit 44 is located remotely from each other, and prevents the pipette tips placed in multiple samples and pipette tips unit 44 in sample unit 43 from intersecting dirt Dye avoids pipette tips from being polluted by the sample in sample unit 43, improves nucleic acid extraction precision.
In one embodiment, the controller in nucleic acid extraction instrument 100 is additionally operable to control liquid absorption device 60 and draws liquid Secondary returning suction is carried out after leaving liquid level, prevents pipette tips extension from polluting sample in workbench 10 and sample unit 43 etc. after dripping, into one Step improves the precision of nucleic acid extraction, realizes high-precision automatic nucleic acid extraction.
In one embodiment, nucleic acid extraction instrument 100 is further included positioned at 44 side of pipette tips unit and set on second area PCR construction units 70 in 14, liquid absorption device 60 can move between extraction unit 42 and PCR construction units 60, so can be with The nucleic acid extracted is moved to PCR construction units 60 by liquid absorption device 60, in case copy detection of next step etc..
In one embodiment, nucleic acid extraction instrument 100 further includes barcode scanner 80, and barcode scanner 80 is set on work On platform 10 and positioned at side of the sample unit 43 far from extraction unit 42, sample unit 43 is put by the record of barcode scanner 80 Each sample information, convenient for tracking and recording the information of each sample.
In one embodiment, workbench 10 further includes the fourth region between first area 12 and second area 14 18, nucleic acid extraction instrument further includes grabbing device 90, the heating unit being set in the fourth region 18 92, oscillating unit 93 and drop Warm unit 94, grabbing device 90 is used for pick-and-place deep-well plates 422, and grabbing device 90 can be along the first direction in first area 12 and the Move back and forth between four regions 18, grabbing device 90 can also be single in heating unit 92, oscillating unit 93 and cooling in a second direction Move back and forth between member 94.
During nucleic acid is extracted, the deep-well plates 422 in extraction unit 42 can be captured by grabbing device 90 and are moved It is dynamic, the acceleration cracking of heating unit 92 is can be moved to after cracked solution is added in deep-well plates 422, it can also be by grabbing after the completion of cracking Device 90 is taken to capture deep-well plates 422 and is moved to temperature reducing unit 94, accelerates the sample cooling after cracking, deep-well plates 422 can also be grabbed The crawl of device 90 is taken to be moved to oscillating unit 93, oscillating unit 93 can vibrate the solution in mixing deep-well plates 422, thus by shaking Swing the process that unit 93, heating unit 92 and temperature reducing unit 94 accelerate nucleic acid extraction.And by heating unit 92, oscillating unit 93 And temperature reducing unit 94 is set in the fourth region 18 together, the relevant unit of function is positioned in the same area, makes entire workbench Each unit on 10 is compacter, makes nucleic acid extraction instrument 100 is whole more to minimize.
In one embodiment, support element 20 extends in a first direction, and liquid absorption device 60 and grabbing device 90 are removable Ground is set on support element 20, and is set respectively close to sample unit 43 and pipette tips unit 44, so by liquid absorption device 60 and is grabbed Assembling device 90 is taken to be movably arranged on support element 20, it can be by realizing that the absorption of sample moves along moving for support element 20 It is dynamic, and liquid absorption device 60 is convenient for pipette samples close to the setting of sample unit 43, and grabbing device 90 is set close to pipette tips unit 44 It puts, convenient for the deep-well plates 422 on extraction unit 42 to be moved to the heating unit 92 on 44 side of pipette tips unit, temperature reducing unit 94 and are shaken Unit 93 is swung, saves the traveling time of liquid absorption device 60 and grabbing device 90.
Further, liquid absorption device 60 and grabbing device 90 include moving guide rail 61 and executive item 63, moving guide rail 61 It is movably arranged on support element 11 and drives executive item 63 reciprocal along the first direction in first area 12 to third region 16 It is mobile, and moving guide rail 61 extends along the second direction of vertical first direction, and executive item 63 is movably arranged at movement and leads On rail 61, executive item 63 can move along the first direction under the drive of moving guide rail 61, and executive item 63 can be along moving The second direction movement that guide rail 61 extends, such executive item 63 can be in a first direction and in the second direction vertical with first direction It is mobile, realize the imbibition of each position and the crawl of deep-well plates 422.
Further, controller is additionally operable to moving guide rail 61 in control liquid absorption device 60 and executive item 63 respectively along the One direction and second direction are mobile simultaneously, and 63 motion track of executive item can be oblique line or camber line, will not be broken line, reduce movement Time;And/or controller be additionally operable to control grabbing device 90 in moving guide rail 61 and executive item 63 respectively along the first direction It is mobile simultaneously with second direction, so selectively control one or both of liquid absorption device 60 and grabbing device 90 edge simultaneously First direction and second direction movement, save the mobile time.
In one embodiment, garbage area 50 includes pipette tips garbage area 52 and waste 54, during nucleic acid is extracted, After liquid absorption device 50 assembles the absorption that pipette tips complete a kind of sample or reagent, used pipette tips are needed replacing, by discarded rifle Head concentrates on pipette tips garbage area 52, and removal waste liquid is needed during nucleic acid is extracted, and can pass through the waste liquid of generation Liquid absorption device 50 is moved to waste 54, different waste materials is classified, convenient for subsequent processing.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited In contradiction, it is all considered to be the range of this specification record.
Embodiment described above only expresses the several embodiments of the utility model, and description is more specific and detailed, But therefore it can not be interpreted as the limitation to utility model patent range.It should be pointed out that the common skill for this field For art personnel, without departing from the concept of the premise utility, various modifications and improvements can be made, these are belonged to The scope of protection of the utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.

Claims (10)

1. a kind of nucleic acid extraction instrument, which is characterized in that including workbench, support element, exhaust apparatus, nucleic acid extraction module and give up Liquid unit, the support element are set on the workbench side and working space are formed between the workbench, and the nucleic acid carries Modulus block and the waste unit are set on the workbench and in the working space, and the waste unit is close to institute Support element setting is stated, corresponding to the waste unit on the support element sets the exhaust apparatus.
2. nucleic acid extraction instrument according to claim 1, which is characterized in that the nucleic acid extraction module includes reagent unit And extraction unit, the workbench include first area, the reagent unit, the extraction unit and the waste unit are set In in the first area, and the extraction unit is between the reagent unit and the waste unit, the exhaust dress It installs and is placed in the support element and the first area corresponding position.
3. nucleic acid extraction instrument according to claim 2, which is characterized in that controller and liquid absorption device are further included, it is described Nucleic acid extraction module further includes sample unit and pipette tips unit, and the workbench further includes the second area being oppositely arranged and third Region, the first area are set between the second area and the third region, the pipette tips unit and the sample list Member is respectively arranged in the second area and the third region, and the liquid absorption device is controlled according to the controller in first party Move back and forth between the first area, second area and third region upwards, and the liquid absorption device also with it is described It is moved back between the waste unit, the extraction unit and the reagent unit in the second direction that first direction intersects It is dynamic.
4. nucleic acid extraction instrument according to claim 3, which is characterized in that the controller is additionally operable to control the imbibition Device is drawn after liquid leaves liquid surface and carries out secondary returning suction.
5. nucleic acid extraction instrument according to claim 3, which is characterized in that the extraction unit includes being placed with reaction tube Deep-well plates, carrier and magnetic mechanism, the deep-well plates are detachably arranged on the carrier, and the magnetic mechanism is set on described The bar magnet connecting in carrier and including displacement piece and with the displacement piece, the displacement piece be used to driving the bar magnet to close or Direction movement far from the reaction pipe outer wall.
6. nucleic acid extraction instrument according to claim 5, which is characterized in that the workbench is further included positioned at described first The fourth region between region and the second area, the nucleic acid extraction instrument further include grabbing device, are set to described Heating unit, oscillating unit and temperature reducing unit in four regions, the grabbing device is used for deep-well plates described in pick-and-place, and described grabs Take device that can move back and forth between the first area and the fourth region along the first direction, the grabbing device is also It can move back and forth between the heating unit, the oscillating unit and the temperature reducing unit along the second direction.
7. nucleic acid extraction instrument according to claim 6, which is characterized in that the support element prolongs along the first direction It stretches, the liquid absorption device and the grabbing device are movably arranged on the support element, and respectively close to the sample Unit and pipette tips unit setting.
8. nucleic acid extraction instrument according to claim 7, which is characterized in that the liquid absorption device and the grabbing device are equal Including moving guide rail and executive item, the moving guide rail is movably arranged on the support element and drives the executive item edge The first direction moves back and forth, and the moving guide rail extends in a second direction, and the executive item is movably arranged at institute It states on moving guide rail.
9. nucleic acid extraction instrument according to claim 3, which is characterized in that further include positioned at the pipette tips unit side simultaneously PCR construction units in the third region, the liquid absorption device can be in the extraction units and the PCR construction units Between move.
10. nucleic acid extraction instrument according to claim 3, which is characterized in that further include barcode scanner and garbage area, institute Barcode scanner is stated on the workbench and positioned at side of the sample unit far from the extraction unit;It is and described useless Expect that area includes pipette tips garbage area and waste.
CN201721028999.6U 2017-08-16 2017-08-16 Nucleic acid extraction instrument Active CN207468609U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115992039A (en) * 2023-03-21 2023-04-21 深圳市美德瑞生物科技有限公司 Automatic nucleic acid extraction device is inhaled to lower part magnetism

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115992039A (en) * 2023-03-21 2023-04-21 深圳市美德瑞生物科技有限公司 Automatic nucleic acid extraction device is inhaled to lower part magnetism

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Address after: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan

Patentee after: Shengxiang Biotechnology Co., Ltd

Address before: 410205 Changsha province high and New Technology Industrial Development Zone, Lu Pine Road, No. 680, Hunan

Patentee before: Sansure Biotech Inc.

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Address after: No. 680, lushong Road, high tech Industrial Development Zone, Changsha City, Hunan Province, 410205

Patentee after: SANWAY GENE TECH Inc.

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Patentee before: Shengxiang Biotechnology Co.,Ltd.

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