CN211170706U - Nucleic acid extraction instrument for batch samples - Google Patents
Nucleic acid extraction instrument for batch samples Download PDFInfo
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- CN211170706U CN211170706U CN201922091072.2U CN201922091072U CN211170706U CN 211170706 U CN211170706 U CN 211170706U CN 201922091072 U CN201922091072 U CN 201922091072U CN 211170706 U CN211170706 U CN 211170706U
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Abstract
A batch sample nucleic acid extractor comprising: the device comprises a nucleic acid extraction instrument main body, a mechanical arm, a liquid pumping head, a sample placing bottom plate, a vibration heating plate, a sample placing magnetic frame, a waste liquid groove, a liquid pumping head cleaning groove, a reagent liquid adding system, a reagent cleaning system, an X-axis guide rail sliding block, a Y-axis guide rail sliding block, a Z-axis guide rail sliding block and a sample placing plate; a frame is arranged in the nucleic acid extractor main body, a sample placing bottom plate, a sample placing magnetic frame, a waste liquid tank and a liquid pumping head cleaning tank are arranged above the bottom of the nucleic acid extractor main body, and the sample placing plate is arranged above the sample placing bottom plate; the frame is provided with an X-axis guide rail connected through a Y-axis guide rail sliding block, the X-axis guide rail is provided with a mechanical arm connected through an X-axis guide rail sliding block, and the mechanical arm is connected with the mechanical arm through a Z-axis guide rail sliding block; the liquid pumping head and the liquid pumping head are arranged below the manipulator. The utility model discloses a have draw fast, continuous operation, save the advantage of consumptive material.
Description
Technical Field
The utility model relates to a nucleic acid extraction instrument, specific batch sample nucleic acid extraction instrument that says so.
Background
At present, the research on nucleic acid is an important subject in modern biology and medical research. Nucleic acid, which is a carrier of genetic information, is the material basis for gene expression, and has a very important role in the normal growth, development and reproduction of organisms, and other life activities, and is also closely related to life abnormalities, such as tumorigenesis, radiation damage, genetic diseases, and the like.
A nucleic acid extractor is an instrument which automatically finishes the extraction work of sample nucleic acid by using a matched nucleic acid extraction reagent, and the nucleic acid extractor is divided into two types: one type is large-scale automated, commonly referred to as automated liquid workstations; the other type is a small automatic nucleic acid extractor which automatically finishes the extraction and purification process by using a packaged matched reagent. The large automatic liquid workstation is high in equipment cost and operation cost, and is suitable for extracting a plurality of different samples at one time, so that the large automatic liquid workstation is less in application rate. The small-sized automatic instrument is widely applied due to low cost of instrument equipment and operation and convenient operation.
The method for adding magnetic sleeves to the existing magnetic columns in the market. The experimental steps are decomposed into pipetting steps by a machine, and various reagents are pipetted into corresponding centrifugal tubes by a pipetting mechanism according to requirements. The efficiency is low, and the sample can be polluted because the pipette tips are not replaced among the centrifuge tube hole sites. The existing extraction method is a magnetic bar magnetic bead adsorption method, the steps are complex, and special consumables are required to be prepared.
At present, the following defects and shortcomings exist in the common nucleic acid extractor in the market: (1) the flux can not be adjusted flexibly by the matched consumable, and a large amount of time is wasted when various samples are processed. (2) The whole machine can not be operated all the time without stopping, and when the flux of a sample to be processed is too large, the experimental requirement can not be flexibly met. (3) High throughput sample processing results in a large amount of wasted consumables.
SUMMERY OF THE UTILITY MODEL
To the above-mentioned defect of prior art, the utility model provides a pair of batch sample nucleic acid extraction appearance has solved above-mentioned technical problem, provides a batch sample nucleic acid extraction appearance that uses 96 orifice plates magnetic frame, and it is good to have stability, saves the characteristics of consumptive material.
In order to achieve the above object, the utility model provides a following technical scheme:
a batch sample nucleic acid extractor comprising: the device comprises a nucleic acid extraction instrument main body, a mechanical arm, a liquid pumping head, a sample placing bottom plate, a vibration heating plate, a sample placing magnetic frame, a waste liquid groove, a liquid pumping head cleaning groove, a reagent liquid adding system, a reagent cleaning system, an X-axis guide rail sliding block, a Y-axis guide rail sliding block, a Z-axis guide rail sliding block and a sample placing plate; a frame is arranged in the nucleic acid extractor main body, a sample placing bottom plate, a sample placing magnetic frame, a waste liquid tank and a liquid pumping head cleaning tank are arranged above the bottom of the nucleic acid extractor main body, and the sample placing plate is arranged above the sample placing bottom plate; the frame is provided with an X-axis guide rail connected through a Y-axis guide rail sliding block, the X-axis guide rail is provided with a mechanical arm connected through an X-axis guide rail sliding block, and the mechanical arm is connected with the mechanical arm through a Z-axis guide rail sliding block; the liquid pumping head is arranged below the mechanical arm, the liquid pumping head is connected to the mechanical arm through a lead screw guide rail, and the liquid pumping head is connected with a reagent liquid feeding system and a reagent cleaning system through pipelines.
The Y-axis guide rails are respectively arranged on the end faces above the two sides of the frame, two Y-axis guide rail sliders slide on the Y-axis guide rails synchronously, and the Y-axis guide rail sliders are connected to the two ends of the X-axis guide rail.
The X-axis guide rail is provided with a slidable X-axis guide rail sliding block, the X-axis guide rail sliding block is connected with a mechanical arm, a vertical Z-axis guide rail is arranged on the mechanical arm, a Z-axis guide rail sliding block is arranged on the Z-axis guide rail, and the Z-axis guide rail sliding block is connected with a mechanical arm; the X-axis guide rail sliding block slides along the Y-axis guide rail through the Y-axis guide rail sliding block, and the mechanical arm slides along the X-axis guide rail through the X-axis guide rail sliding block; the manipulator slides along the Z-axis guide rail through the Z-axis guide rail sliding block, and the manipulator grabs the sample placing plate.
The liquid pumping head is fixed at the bottom of the mechanical arm through a connecting plate, the reagent liquid feeding system and the reagent cleaning system supply liquid to the liquid pumping head through pipelines, the liquid pumping head is connected with a vacuum waste liquid barrel, and the vacuum waste liquid barrel pumps waste liquid in the liquid pumping head; the mechanical arm is connected with the Z-axis guide rail sliding block, a lead screw nut is arranged on the Z-axis guide rail sliding block and connected with a lead screw (a positive lead screw and a negative lead screw), a liquid pumping head is arranged on the lead screw, the lead screw rotates to drive the mechanical arm and the liquid pumping head to move, and the mechanical arm and the liquid pumping head move reversely.
The sample placing bottom plate, the oscillating heating plate and the sample placing magnetic frame are all provided with sample placing plates, the sample placing plates are provided with test solution extracting holes, and the number of the test solution extracting holes is 96 to form a matrix to be arranged on the sample placing plates.
The reagent liquid adding system comprises a reagent bottle I and an electromagnetic valve, wherein the reagent bottle I is connected with a liquid injecting head through a pipeline, and the electromagnetic valve is arranged on the pipeline.
The reagent cleaning system comprises a reagent bottle II and an electromagnetic valve, wherein the reagent bottle II conveys liquid for cleaning the liquid pumping head into the liquid pumping head and is used for cleaning the inside of the liquid pumping head, and the electromagnetic valve controls the reagent bottle II.
The work of each part of the nucleic acid extraction instrument main body is controlled by a software workstation, and the software workstation is arranged outside the nucleic acid extraction instrument main body and is connected with the nucleic acid extraction instrument main body through a line.
The waste liquid tank and the liquid pumping head cleaning tank are of an integrated structure, a partition plate is arranged between the waste liquid tank and the liquid pumping head cleaning tank, and the waste liquid tank and the liquid pumping head cleaning tank pump waste liquid through liquid pumping pumps connected with the waste liquid tank and the liquid pumping head cleaning tank.
Wherein, vibrate the hot plate and include: variable speed centrifugation vibrates motor and resistance heating board, resistance heating board sets up in variable speed centrifugation vibration motor top, it places the board to be equipped with the sample on the resistance heating board.
The utility model has the advantages that: the utility model provides a pair of sample nucleic acid extraction appearance in batches has and draws nucleic acid in batches, vibrates evenly, the advantage of quick drawing liquid. The liquid pumping head adopts the variable-diameter liquid pumping head, so that the liquid is completely pumped out. The magnetic column is arranged on the sample placing magnetic frame, and the guide plate is also arranged at the sample placing magnetic frame, so that the accuracy of sample placement is ensured, and frequent calibration is not needed; the sample placing plate adopts a 96-hole deep hole plate, so that the treatment of more samples can be adapted, and the time is saved; the batch sample nucleic acid extractor is provided with a plurality of sample placing bottom plates, a vibration heating plate and a sample placing magnetic frame, and samples can be continuously extracted without stopping through scientific sequencing; magnetic beads are not used in sample extraction, a steel needle of the liquid injection head does not need to be replaced, and consumables are saved.
For a better understanding of the features and technical content of the present invention, reference should be made to the following detailed description of the present invention and accompanying drawings, which are provided for the purpose of illustration and description and are not intended to limit the present invention.
Drawings
The technical solution and other advantages of the present invention will become apparent from the following detailed description of the embodiments of the present invention with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of the internal structure of a batch sample nucleic acid extractor of the present invention;
FIG. 2 is a schematic diagram of a waste liquid tank and a liquid suction head cleaning tank of the batch sample nucleic acid extractor of the present invention;
FIG. 3 is a schematic view of a main body of a nucleic acid extractor of the present invention;
fig. 4 is a partially enlarged view of fig. 1.
Reference numerals:
1: a nucleic acid extractor body; 2: a mechanical arm; 3: a liquid injection head; 4: a liquid pumping head; 5: a sample placement floor; 6: vibrating the heating plate; 7: placing a sample on a magnetic frame; 8: a waste liquid tank; 9: a liquid pumping head cleaning tank; 10: a Y-axis guide rail slider; 11: a sample placement plate; 12: a frame; 13: a manipulator; 14: an X-axis guide rail; 15: a Y-axis guide rail; (ii) a 17: a connecting plate; 18: a steel needle; 19: a first connection hole; 20: a liquid-extracting needle tube; 21: a second connection hole; 22: a sample solution extraction well; 23; a sloping plate; 24: a magnetic column.
Detailed Description
To further illustrate the technical means and effects of the present invention, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings.
Referring to fig. 1 to 4, a batch sample nucleic acid extractor includes: the device comprises a nucleic acid extraction instrument main body 1, a mechanical arm 2, a mechanical arm 13, a liquid pumping head 3, a liquid pumping head 4, a sample placing bottom plate 5, a vibration heating plate 6, a sample placing magnetic frame 7, a waste liquid groove 8, a liquid pumping head cleaning groove 9, a reagent liquid adding system, a reagent cleaning system, an X-axis guide rail slide block (not marked in the figure), a Y-axis guide rail slide block 10, a Z-axis guide rail slide block (not marked in the figure) and a sample placing plate 11; a frame 12 is arranged in the nucleic acid extractor main body 1, a sample placing bottom plate 5, a sample placing magnetic frame 7, a waste liquid groove 8 and a liquid pumping head cleaning groove 9 are arranged above the bottom of the nucleic acid extractor main body 1, and the sample placing plate 11 is arranged above the sample placing bottom plate 5; an X-axis guide rail 14 connected through a Y-axis guide rail slider 10 is arranged on the frame 12, a mechanical arm 2 connected through an X-axis guide rail slider is arranged on the X-axis guide rail 14, and the mechanical arm 2 is connected with a mechanical arm through a Z-axis guide rail slider; the liquid pumping head 3 is arranged below the manipulator 13, the liquid pumping head 4 is connected to the manipulator 2 through a lead screw guide rail, and the liquid pumping head 3 is connected with a reagent liquid feeding system and a reagent cleaning system through pipelines.
Further, the upper end faces of the two sides of the frame 12 are respectively provided with a Y-axis guide rail 15, the two Y-axis guide rail sliders 10 slide on the Y-axis guide rails 15 synchronously, and the Y-axis guide rail sliders 10 are connected to the two ends of the X-axis guide rail 14.
The Y-axis guide rail sliders 10 arranged on the Y-axis guide rails 15 are controlled by a motor, so that the Y-axis guide rail sliders 10 move back and forth along the Y-axis guide rails 15, and the two Y-axis guide rail sliders 10 on the two Y-axis guide rails 15 move synchronously. The commands executed by the motor are issued by a software workstation.
Further, a slidable X-axis guide rail slider is arranged on the X-axis guide rail 14, the X-axis guide rail slider is connected with the mechanical arm 2, a vertical Z-axis guide rail is arranged on the mechanical arm 2, a Z-axis guide rail slider is arranged on the Z-axis guide rail, and the Z-axis guide rail slider is connected with the manipulator 13; the X-axis guide rail sliding block slides along a Y-axis guide rail 15 through a Y-axis guide rail sliding block 10, and the mechanical arm 2 slides along an X-axis guide rail 14 through an X-axis guide rail sliding block; the manipulator 13 slides along the Z-axis guide rail through the Z-axis guide rail sliding block, and the manipulator 13 grabs the sample placing plate 11.
The X-axis guide rail sliding block, the Y-axis guide rail sliding block 10 and the Z-axis guide rail sliding block are controlled to slide through motors.
Further, the liquid pumping head 3 is fixed at the bottom of the mechanical arm 2 through a connecting plate 17, the reagent liquid feeding system and the reagent cleaning system supply liquid to the liquid pumping head 3 through pipelines, the liquid pumping head 4 is connected with a vacuum waste liquid barrel, and the vacuum waste liquid barrel pumps waste liquid in the liquid pumping head 4; the mechanical arm 13 is connected with the Z-axis guide rail sliding block, a lead screw nut is arranged on the Z-axis guide rail sliding block and connected with a lead screw, a liquid pumping head is arranged on the lead screw, the lead screw rotates to drive the mechanical arm 13 and the liquid pumping head 4 to move, and the mechanical arm 13 and the liquid pumping head 4 move reversely.
The first row of connecting holes and the second row of connecting holes of the liquid pumping head 3 are respectively connected with a reagent cleaning system and a reagent liquid feeding system; the first connecting hole 19 is arranged on the side surface and used for connecting an air pipeline, so that air can be conveniently injected.
The reagent liquid feeding system provides reagent for the steel needle 18 of the liquid beating head 3, and after the reagent is released by the steel needle beating liquid, cleaning liquid is beaten into the steel needle 18 of the liquid beating head 3 through the reagent cleaning system, so that clear water can be selected for use, the residual reagent in the steel needle is cleaned out, and the metering of the next steel needle beating liquid is convenient to be consistent.
The liquid extraction needle tube 20 is connected below the liquid extraction head 4, the two ends of the liquid extraction head 4 are provided with second connecting holes 21, the second connecting holes 21 are connected with the vacuum waste liquid barrel, and the vacuum waste liquid barrel pumps the liquid extracted from the liquid extraction head 4 into the vacuum waste liquid barrel through the second connecting holes 21.
The liquid pumping head 3 is fixed on the mechanical arm 2, the mechanical arm 13 and the liquid pumping head 4 move oppositely, and when the mechanical arm 13 moves downwards, the liquid pumping head 4 moves upwards; the liquid pumping head 4 is connected with the mechanical hand 13 through a positive screw and a negative screw, and when the mechanical hand 13 moves downwards, the liquid pumping head 4 moves upwards.
Further, all can place the sample on sample placement bottom plate 5, shock hot plate 6 and the sample is placed magnetic frame 7 and place board 11, the sample is placed and is equipped with test solution extraction hole 22 on the board 11, test solution extraction hole 22 is arranged on board 11 is placed to the sample for the matrix.
The sample placing magnetic frame 7 is fixed with the sample placing plate 11, and the sample placing magnetic frame 7 is composed of a sample placing plate 11 placing guide structure and a magnetic adsorption structure. The guide structure is vertical swash plate 23, and magnetic adsorption structure places magnetic frame 7 through the sample and goes up magnetic column 24 and realize, and sample is placed board 11 below and is equipped with the magnetic structure of inhaling mutually with magnetic column 24.
The sample placing plate 11 is a 96-deep well plate, and 96 sample solution extraction wells are provided on the sample placing plate 11.
Further, reagent liquid feeding system includes reagent bottle I and solenoid valve, reagent bottle I passes through the tube coupling and beats liquid head 3, be equipped with the solenoid valve on the pipeline.
The reagent liquid adding system is connected with the reagent bottle I and the electromagnetic valve through pipelines to form a set of pressurized liquid adding system.
Further, reagent cleaning system includes reagent bottle II and solenoid valve, reagent bottle II passes through the tube coupling and beats liquid head 3, for beating liquid head 3 and carry the washing liquid, washs 18 inner chambers of the steel needle of beating liquid head 3.
Further, the work of each part of the nucleic acid extractor main body 1 is controlled by a software workstation, and the software workstation is arranged outside the nucleic acid extractor main body 1 and is connected by a line.
Further, waste liquid groove 8 and drawing liquid head washing tank 9 formula structure as an organic whole are equipped with the baffle between waste liquid groove 8 and the drawing liquid head washing tank 9, liquid in waste liquid groove 8 and the drawing liquid head washing tank 9 is taken out through the drawing liquid pump, the drawing liquid pump sets up outside nucleic acid extraction appearance main part 1, and the drawing liquid mouth setting of drawing liquid pump is in waste liquid groove 8 and drawing liquid head washing tank 9.
Further, the oscillating heating plate 6 includes: variable speed centrifugation vibrates motor and resistance heating board, resistance heating board sets up in variable speed centrifugation vibration motor top, it places board 11 to be equipped with the sample on the resistance heating board.
The variable speed centrifugal oscillation motor is formed by connecting an oscillation motor, an oscillation eccentric shaft and an eccentric bearing seat.
Example (b):
the top of the bottom of the nucleic acid extractor main body 1 is provided with two sample placing bottom plates 5, four sample placing magnetic frames 7 and two vibrating heating plates 6. Two sample placing magnetic frames 7, two sample placing bottom plates 5, two sample placing magnetic frames 7 and two vibrating heating plates 6 are placed in sequence.
The software workstation controls a motor to work, the motor controls the X-axis guide rail sliding block, the Y-axis guide rail sliding block 10 and the Z-axis guide rail sliding block to be linked, and a sample placing plate 11 is placed on the sample placing bottom plate 5 below the manipulator 13. The X-axis guide rail slide block moves left and right on the X-axis guide rail 14, the Y-axis guide rail slide block 10 moves back and forth on the Y-axis guide rail 15, the position of the mechanical arm 2 is adjusted to the waste liquid tank 8 through the X-axis guide rail slide block and the Y-axis guide rail slide block 10, and residual liquid in a steel needle 18 of a liquid injection head below the mechanical arm 2 enters the waste liquid tank 8 to clean the steel needle 18.
The reagent cleaning system conveys cleaning liquid, such as water, to the steel needle 18 of the liquid injection head 3 through the liquid conveying pump, residual liquid in the steel needle 18 is discharged, and air is used for blowing the inner cavity of the steel needle 18, so that the consistent injection amount of the steel needle into the test liquid extraction hole 22 is ensured next time.
When the steel needle 18 is used for injecting liquid, the steel needle does not extend into the test solution extraction hole 22, so that the outer wall of the steel needle 18 does not need to be cleaned.
The quantity of the steel needle of liquid beating head 3 is 8, and adjacent steel needle distance is 9 millimeters, and the structure of liquid beating head 3 has multiple kind of reagent union coupling connecting hole, can carry out the interpolation of multiple kind of reagent, and liquid beating head 3 upper portion is two lines of reagent union coupling holes, and the hole interval of every line is 9 millimeters, and the line interval of every line is 10 millimeters. The number of holes in the first row is 9 and the number of holes in the second row is 8.
After the steel needle 18 of the liquid injection head 3 is cleaned, the electromagnetic valve of the reagent liquid injection system controls the reagent of the reagent liquid injection system to flow into the steel needle 18, the steel needle 18 moves to the position above the test liquid extraction hole of the sample placing plate 11, reagent liquid is injected, the mechanical arm 13 moves upwards through the Z-axis guide rail slide block, and after the steel needle 18 leaves the test liquid extraction hole, the mechanical arm 13 is controlled by the mechanical arm 13 to move to the waste liquid groove 8 for cleaning again. The reciprocating operation requires cleaning of the inner wall of the steel needle 18 each time the reagent is released.
After the reagent to all samples is added, place the board 11 through manipulator 13 with the sample and grab up, remove to shaking hot plate 6, software workstation control shakes and shakes hot plate 6 and begin work, shakes and mixes the sample through shaking and heating. Because the centrifugal oscillation motor is a variable-speed centrifugal oscillation motor, the oscillation speed and the oscillation mode of the variable-speed centrifugal oscillation motor can be controlled through the software workstation.
The sample which is vibrated and mixed is picked up by a manipulator 13 and moved to a sample placing magnetic frame 7 for magnetic attraction.
The sample placing magnetic frame 7 is provided with a plurality of magnetic columns 24 which are arranged in a matrix manner, and when the sample placing plate 11 is placed above the sample placing magnetic frame 7, the magnetic columns 24 are arranged below the sample to magnetically attract the sample.
After the completion of magnetism, absorbing sample through drawing liquid head 4 and placing sample on board 11 and sample test liquid, drawing liquid head 4 moves down, and positive and negative screw connection's manipulator 13 rebound, the first 3 immobilizes of beating of connecting in arm 13 below. The liquid extraction head 4 enters the test liquid extraction hole 22 to extract liquid, the liquid extraction head 4 is cleaned in the same manner as the liquid extraction head 3, the mechanical arm 2 is moved to the liquid extraction head cleaning tank 9, the liquid extraction head 4 moves downwards to the liquid extraction head cleaning tank 9 to clean the outer wall of the liquid extraction head 4, the liquid extraction head 4 is moved to the upper side of the sample placing plate 11 to extract liquid for the sample test liquid after the cleaning is completed, the liquid can be extracted again after the liquid extraction head is cleaned in the liquid extraction head cleaning tank 9 after one time, and waste liquid in the liquid extraction head 4 enters a vacuum waste liquid barrel connected with the liquid extraction head.
The number of the liquid extracting needle tubes 20 is the same as that of the steel needles 18, the inner diameter of each liquid extracting needle tube is 0.5-0.8 mm, preferably 0.7mm, and a variable-diameter suction head device is arranged at the needle head position of each liquid extracting needle tube 20. The liquid-pumping needle tube 20 is connected with a vacuum waste liquid barrel through a second connecting hole 21, and the vacuum waste liquid barrel forms negative pressure in the liquid-pumping needle tube 20 to perform extraction of waste liquid.
The top of 1 bottom of nucleic acid extraction appearance main part is equipped with two samples that the order was put and places magnetic frame 7, board 11 is placed to two samples, magnetic frame 7 and two vibrations hot plate 6 are placed to two samples, after the extraction of sample was accomplished, place magnetic frame 7 with the sample through manipulator 13, because the sample is placed the quantity of magnetic frame 7 and is divided into two sets ofly for four, consequently, the sample after the extraction is when placing sample magnetic frame 7, the work that has unnecessary sample to place magnetic frame 7 and can guarantee to extract the sample continues to go on.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. A batch sample nucleic acid extractor, comprising: nucleic acid extraction appearance main part (1), direction slider, mechanical control device, sample frame, beat liquid belt cleaning device, control system and waste liquid washing tank, be equipped with mechanical control device in the nucleic acid extraction appearance main part (1), nucleic acid extraction appearance main part (1) bottom is equipped with sample frame and waste liquid washing tank, mechanical control device connects the direction slider.
2. The apparatus for batch sample nucleic acid extraction according to claim 1, wherein the guide slider comprises: an X-axis guide rail sliding block, a Y-axis guide rail sliding block (10), a Z-axis guide rail sliding block, an X-axis guide rail (14), a Y-axis guide rail (15) and a Z-axis guide rail; a frame (12) is arranged in the nucleic acid extractor main body (1); the mechanical control device includes: a robot arm (2) and a manipulator (13); a mechanical arm (13) is arranged on the mechanical arm (2); an X-axis guide rail (14) connected through a Y-axis guide rail sliding block (10) is arranged on the frame (12), a mechanical arm (2) connected through an X-axis guide rail sliding block is arranged on the X-axis guide rail (14), and a Z-axis guide rail sliding block is arranged on the mechanical arm (2); y-axis guide rails (15) are respectively arranged on the upper end faces of two sides of the frame (12), two Y-axis guide rail sliding blocks (10) synchronously slide on the Y-axis guide rails (15), and the Y-axis guide rail sliding blocks (10) are connected to two ends of the X-axis guide rail (14); an X-axis guide rail sliding block capable of sliding is arranged on the X-axis guide rail (14), the X-axis guide rail sliding block is connected with the mechanical arm (2), a vertical Z-axis guide rail is arranged on the mechanical arm (2), a Z-axis guide rail sliding block is arranged on the Z-axis guide rail, and the Z-axis guide rail sliding block is connected with the mechanical arm (13); the X-axis guide rail sliding block slides along a Y-axis guide rail (15) through a Y-axis guide rail sliding block (10), and the mechanical arm (2) slides along an X-axis guide rail (14) through an X-axis guide rail sliding block; the manipulator (13) slides along the Z-axis guide rail through the Z-axis guide rail sliding block, and the manipulator (13) grabs the sample placing plate (11).
3. The apparatus according to claim 1, wherein the sample holder comprises: a sample placing bottom plate (5), a vibration heating plate (6), a sample placing magnetic frame (7) and a sample placing plate (11); the sample is placed bottom plate (5), is vibrate hot plate (6) and sample and is placed sample and place board (11) on placing magnetic frame (7), the sample is placed and is equipped with test solution extraction hole (22) on board (11), test solution extraction hole (22) are arranged on board (11) is placed to the sample for the matrix.
4. The apparatus for batch sample nucleic acid extraction according to claim 3, wherein the oscillating heating plate (6) comprises: variable speed centrifugation vibrates motor and resistance heating board, resistance heating board sets up in the centrifugation and vibrates motor top, be equipped with sample on the resistance heating board and place board (11).
5. The apparatus according to claim 2, wherein the washing and cleaning device comprises: a liquid injection head (3) and a liquid extraction head (4); the liquid pumping head (3) is connected below the mechanical arm (2) through a connecting plate (17), the liquid pumping head (4) and the mechanical arm (13) move in a reverse direction, the liquid pumping head (4) is connected with a vacuum waste liquid barrel, and the vacuum waste liquid barrel pumps waste liquid in the liquid pumping head; the liquid pumping head (4) is a variable-diameter liquid pumping head.
6. The apparatus according to claim 5, wherein the control system comprises: the system comprises a reagent liquid adding system, a reagent cleaning system and a software workstation; the reagent liquid adding system comprises a reagent bottle I and an electromagnetic valve, the reagent bottle I is connected with the liquid pumping head (3) through a pipeline, the electromagnetic valve is arranged on the pipeline, the reagent liquid adding system and the reagent cleaning system supply liquid for the liquid pumping head (3) through the pipeline, the reagent cleaning system comprises a reagent bottle II and an electromagnetic valve, and the reagent bottle II is connected with the liquid pumping head (3) through the pipeline and provides cleaning liquid for the liquid pumping head; the software workstation controls each part of the nucleic acid extractor main body (1); the software workstation is arranged outside the nucleic acid extractor main body (1) and is connected with the nucleic acid extractor main body through a line.
7. The apparatus as claimed in claim 1, wherein the waste liquid tank comprises a waste liquid tank (8) and a liquid-pumping head tank (9), the waste liquid tank (8) and the liquid-pumping head tank (9) are of an integral structure, a partition is disposed between the waste liquid tank (8) and the liquid-pumping head tank (9), and the waste liquid tank (8) and the liquid-pumping head tank (9) draw the waste liquid by a pipe-liquid pump.
8. The apparatus for extracting nucleic acid from a batch of samples according to claim 1, wherein a sample rack and a waste solution washing tank are arranged above the bottom of the main body (1) of the apparatus.
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| CN201922091072.2U CN211170706U (en) | 2019-11-28 | 2019-11-28 | Nucleic acid extraction instrument for batch samples |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922091072.2U CN211170706U (en) | 2019-11-28 | 2019-11-28 | Nucleic acid extraction instrument for batch samples |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022088472A1 (en) * | 2020-10-27 | 2022-05-05 | 广州和实生物技术有限公司 | Integrated nucleic acid detection workstation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022088472A1 (en) * | 2020-10-27 | 2022-05-05 | 广州和实生物技术有限公司 | Integrated nucleic acid detection workstation |
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