CN214830378U - Biological experiment operating equipment - Google Patents
Biological experiment operating equipment Download PDFInfo
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- CN214830378U CN214830378U CN202120801122.6U CN202120801122U CN214830378U CN 214830378 U CN214830378 U CN 214830378U CN 202120801122 U CN202120801122 U CN 202120801122U CN 214830378 U CN214830378 U CN 214830378U
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Abstract
The utility model discloses a biology experiment operating equipment, this biology experiment operating equipment, including workstation and lateral shifting module, workstation one side is fixed with the lateral shifting module, and the opposite side of workstation is fixed with moves back the suction head storehouse. The utility model relates to a biological experiment operating equipment, this biological experiment operating equipment contain and move liquid, vibration, heating, magnetism and inhale, abandon liquid and ultrasonic cleaning etc. experiment operation's method and equipment, and this solution has good openness, flexibility and expansibility, has integrated the functional module of multiple difference, can realize the biological experiment operation of multiple scheme, and the experimental result is reliable, and good repeatability effectively increases the flux, promotes work efficiency, can reduce experiment consumptive material consumption simultaneously by a wide margin.
Description
Technical Field
The utility model relates to biological equipment technical field, in particular to biological experiment operating equipment.
Background
The automatic pipetting workstation is an automatic operation platform of a biological medicine laboratory, which skillfully integrates automatic operation and liquid treatment, can realize the operations of automatic sample adding, reagent distribution, ultramicro pipetting and the like of biological samples, and can be widely applied to biological operation experiments in various fields such as nucleic acid detection, gene synthesis, disease control, forensic medicine identification, environmental microorganism detection, food safety detection and the like.
Most of the existing automatic pipetting workstations only have a simple pipetting function, can replace simple and repeated pipetting operation, but have single functions, consume large consumable materials, can only provide pipetting partial operation and cannot provide complete experimental operation for more complicated experimental operation.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art, the utility model provides a biology experiment operating equipment to it is great to solve original structure volume, problem with high costs in the use.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a biology experiment operation equipment, includes workstation and lateral shifting module, workstation one side is fixed with the lateral shifting module, the opposite side of workstation is fixed with moves back the suction head storehouse, be equipped with the longitudinal shifting module on the lateral shifting module, be equipped with on the longitudinal shifting module and move liquid module, the middle part of workstation is equipped with a plurality of suction head supports and 96 orifice plate rack, be equipped with test tube rack on suction head support and the left workstation of 96 orifice plate rack, be equipped with low temperature reagent rack and PCR board rack on the workstation on suction head support and 96 orifice plate rack right side, be equipped with operating module, abandon liquid and cleaning module and liquid feeding module on the workstation on low temperature reagent rack right side.
Preferably, the pipetting module is provided with four independently controlled precise plunger pump pipettors, the range is adjustable, 0.5 ml liquid sampling can be completed at minimum, and a pipetting mode with variable intervals can be provided.
Preferably, a deep hole plate clamping seat, a heating assembly, a magnetic suction assembly and a vibration blending assembly are arranged in the operation module, the operation module internally comprises a positioning function, the position is fixed in a stop state every time, and the magnetic suction device can move up and down; the heating of the heating component adopts a fin heating design, the heating of the heating component is uniform, and the heating can be accurately controlled at 0-100 ℃.
Preferably, 96 channels are provided in the discard and wash module, which can be moved into the sample plate, and the discard and wash module can aspirate waste liquid under negative pressure.
Preferably, the liquid adding module is provided with five channel pipelines, the liquid adding module can independently control five reagents through the channel pipelines, and each channel pipeline is provided with a liquid spraying nozzle of twelve branch pipes.
The using method of the biological experiment operating equipment is characterized by comprising the following steps:
1) directly placing a sample collection tube to be extracted into a sample rack of the test tube placing rack, and moving the sample in the sample collection tube into an extraction plate in an operation module through a liquid transfer module, wherein 96 samples can be moved into the extraction plate at most;
2) the liquid further adding module is moved to the position above an extraction plate in the operation module through a mechanical arm, lysate is added, protease K is added through the liquid transferring module, then the operation module executes oscillation operation, samples are uniformly mixed, meanwhile, the temperature is raised to 60 ℃, incubation is carried out for 10 minutes, and the temperature is closed;
3) further, adding magnetic beads through a pipetting module, and performing oscillation operation on an operation module for 1 minute; then, the magnetic frame assembly of the operation module ascends to adsorb magnetic beads to the pipe wall; then the waste liquid and cleaning module is moved to the upper part of the extraction plate, the needle head extends into the bottom position of the inner tube of the extraction plate, the waste liquid extraction operation is executed, the solution in the extraction plate is discharged, then the waste liquid and cleaning module is lifted, and the magnetic frame assembly in the operation module is lowered to the original position;
4) further, the liquid adding module adds protein removing liquid into the extraction plate, then the operation module executes oscillation operation, and after oscillation and uniform mixing, the operation module magnetic frame assembly ascends to adsorb magnetic beads to the tube wall; further moving the liquid discarding and cleaning module to the upper part of the extraction plate, then extending a needle of the liquid discarding and cleaning module to the bottom of the tube in the extraction plate, performing liquid waste extracting and distributing operation, discharging the solution in the extraction plate, then lifting the liquid discarding and cleaning module, and then lowering the magnetic frame assembly of the operation module to the original position;
5) further, adding a washing solution into the extraction plate in a liquid adding module, then operating the module block to execute oscillation operation, and oscillating and uniformly mixing the sample; then the magnetic frame component of the operation module ascends to adsorb magnetic beads to the pipe wall; further moving the liquid discarding and cleaning module to the upper part of the extraction plate, discarding the waste liquid, then lifting and returning the liquid discarding and cleaning module, and lowering the magnetic rack assembly of the operation module to the original position; repeating the steps once, and then operating the module heating assembly to heat up to 60 ℃, and keeping the temperature and drying for 5 minutes;
6) further, the liquid discarding and cleaning module executes ultrasonic cleaning operation, and after cleaning is finished, the liquid discarding and cleaning module returns to the original position;
7) further, eluent is added into the extraction plate by the liquid adding module, then the operation module executes oscillation operation, the oscillation and the uniform mixing are carried out, and the operation of the nucleic acid extraction experiment is finished;
8) and further, the magnetic frame component of the operation module ascends to adsorb magnetic beads to the tube wall, and then the liquid transferring module transfers the sample to a sample tube or a PCR plate of the test tube placing frame according to a preset amount.
The utility model relates to a biological experiment operating equipment, this biological experiment operating equipment contain and move liquid, vibration, heating, magnetism and inhale, abandon liquid and ultrasonic cleaning etc. experiment operation's method and equipment, and this solution has good openness, flexibility and expansibility, has integrated the functional module of multiple difference, can realize the biological experiment operation of multiple scheme, and the experimental result is reliable, and good repeatability effectively increases the flux, promotes work efficiency, can reduce experiment consumptive material consumption simultaneously by a wide margin.
Drawings
FIG. 1 is a schematic structural view of a biological experiment operation apparatus of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1 at A;
FIG. 3 is a schematic view of the internal structure of a biological experiment operation apparatus of the present invention;
FIG. 4 is an enlarged view of a portion of FIG. 3 at B;
in the figure: 1. a work table; 2. a transverse moving module; 3. a longitudinal moving module; 4. a pipetting module; 5. placing a test tube rack; 6. a suction head bracket and a 96-pore plate placing rack; 7. placing a low-temperature reagent rack; 8. placing a PCR plate rack; 9. an operation module; 10. a liquid discarding and cleaning module; 11. a liquid adding module; 12. and (4) withdrawing the suction head bin.
Detailed Description
In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.
Detailed description of the preferred embodimentsa biological experiment operating apparatus, as shown in fig. 1-4, includes a table 1 and a traverse module 2, a transverse moving module 2 is fixed on one side of the workbench 1, a suction head withdrawing bin 12 is fixed on the other side of the workbench 1, the transverse moving module 2 is provided with a longitudinal moving module 3, the longitudinal moving module 3 is provided with a liquid transferring module 4, a plurality of suction head brackets and a 96-pore plate placing rack 6 are arranged in the middle of the workbench 1, a test tube placing rack 5 is arranged on the workbench 1 at the left side of the suction head brackets and the 96-pore plate placing rack 6, a low-temperature reagent placing rack 7 and a PCR plate placing rack 8 are arranged on the workbench 1 on the right side of the suction head bracket and the 96-pore plate placing rack 6, an operating module 9, a liquid abandoning and cleaning module 10 and a liquid adding module 11 are arranged on the workbench 1 on the right side of the low-temperature reagent placing rack 7.
Preferably, the pipetting module 4 is provided with four independently controlled precise plunger pump pipettors, the range is adjustable, 0.5 ml liquid sampling can be completed at minimum, and a pipetting mode with variable intervals can be provided.
Preferably, a deep hole plate clamping seat, a heating assembly, a magnetic suction assembly and a vibration blending assembly are arranged in the operation module 9, the operation module 9 has a positioning function, the position is fixed in a stop state every time, and the magnetic suction device can move up and down; the heating of the heating component adopts a fin heating design, the heating of the heating component is uniform, and the heating can be accurately controlled at 0-100 ℃.
Preferably, 96 channels are provided in the discard and wash module 10, which can be moved into the sample plate, and the discard and wash module 10 can aspirate waste liquid under negative pressure.
Preferably, the liquid adding module 11 is provided with five channel pipelines, the liquid adding module 11 can independently control five reagents through the channel pipelines, and each channel pipeline is provided with twelve liquid spraying nozzles of branch pipes.
The use method of the biological experiment operation equipment comprises the following steps:
1) directly placing a sample collection tube to be extracted into a sample rack of the test tube placing rack 5, and moving the sample in the sample collection tube into an extraction plate in an operation module 9 through a liquid moving module 4, wherein 96 samples can be moved into the extraction plate at most;
2) the liquid further adding module 11 is moved to the position above an extraction plate in the operation module 9 through a mechanical arm, lysate is added, protease K is added through the liquid transferring module 4, then the operation module 9 executes oscillation operation, samples are uniformly mixed, meanwhile, the temperature is raised to 60 ℃, incubation is carried out for 10 minutes, and the temperature is closed;
3) further, magnetic beads are added through the pipetting module 4, and the operation module 9 executes oscillation operation for 1 minute; then, the magnetic frame assembly of the operation module 9 ascends to adsorb magnetic beads to the tube wall; then the waste liquid and cleaning module 10 is moved to the upper part of the extraction plate, the needle head extends into the bottom position of the inner tube of the extraction plate, the waste liquid extracting operation is executed, the solution in the extraction plate is discharged, then the waste liquid and cleaning module 10 is lifted, and the magnetic frame assembly in the operation module 9 is lowered to the original position;
4) further, the liquid adding module 11 adds protein removing liquid into the extraction plate, then the operation module 9 executes oscillation operation, and after oscillation and uniform mixing, the magnetic frame assembly of the operation module 9 rises to adsorb magnetic beads to the tube wall; further moving the liquid discarding and cleaning module 10 to the upper part of the extraction plate, then extending a needle of the liquid discarding and cleaning module 10 into the bottom position of the inner tube of the extraction plate, executing liquid waste extracting and distributing operation, discharging the solution in the extraction plate, then lifting the liquid discarding and cleaning module 10, and then lowering the magnetic rack assembly of the operation module 9 to the original position;
5) further, washing liquid is added into the extraction plate in the liquid adding module 11, then the operation module 9 executes oscillation operation, and the sample is oscillated and uniformly mixed; then the magnetic frame component of the operation module 9 ascends to adsorb magnetic beads to the pipe wall; further moving the liquid discarding and cleaning module 10 to the upper part of the extraction plate, discarding the waste liquid, then lifting and returning the liquid discarding and cleaning module 10, and lowering the magnetic rack assembly of the operation module 9 to the original position; repeating the steps once, and then operating the module 9 to heat the heating assembly to 60 ℃, and keeping the temperature and drying for 5 minutes;
6) further, the liquid discarding and cleaning module 10 executes ultrasonic cleaning operation, and after cleaning is finished, the liquid discarding and cleaning module 10 is returned;
7) further, eluent is added into the extraction plate by the liquid adding module 11, then the operation module 9 executes oscillation operation, the oscillation and the uniform mixing are carried out, and the operation of the nucleic acid extraction experiment is finished;
8) and further, the magnetic frame component of the operation module 9 ascends to adsorb magnetic beads to the tube wall, and then the liquid transferring module 4 transfers the sample to a sample tube or a PCR plate of the test tube placing frame 5 according to a preset amount.
The utility model relates to a biological experiment operating equipment, this biological experiment operating equipment contain and move liquid, vibration, heating, magnetism and inhale, abandon liquid and ultrasonic cleaning etc. experiment operation's method and equipment, and this solution has good openness, flexibility and expansibility, has integrated the functional module of multiple difference, can realize the biological experiment operation of multiple scheme, and the experimental result is reliable, and good repeatability effectively increases the flux, promotes work efficiency, can reduce experiment consumptive material consumption simultaneously by a wide margin.
It is noted that, herein, relational terms such as first and second (a, b, etc.) and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A biological experiment operating device comprises a workbench (1) and a transverse moving module (2), and is characterized in that: a transverse moving module (2) is fixed on one side of the workbench (1), a suction head withdrawing bin (12) is fixed on the other side of the workbench (1), a longitudinal moving module (3) is arranged on the transverse moving module (2), a liquid transferring module (4) is arranged on the longitudinal moving module (3), the middle part of the workbench (1) is provided with a plurality of suction head brackets and 96-pore plate placing racks (6), the suction head bracket and the workbench (1) on the left side of the 96-pore plate placing rack (6) are provided with test tube placing racks (5), a low-temperature reagent placing rack (7) and a PCR plate placing rack (8) are arranged on the workbench (1) on the right side of the suction head bracket and the 96-pore plate placing rack (6), an operation module (9), a liquid abandoning and cleaning module (10) and a liquid adding module (11) are arranged on the workbench (1) on the right side of the low-temperature reagent placing frame (7).
2. A biological laboratory manipulator according to claim 1, wherein: the pipetting module (4) is provided with four independently controlled precise plunger pump pipettors, the range is adjustable, 0.5 ml liquid sampling can be completed at minimum, and a pipetting mode with variable intervals can be provided.
3. A biological laboratory manipulator according to claim 1, wherein: the operation module (9) is internally provided with a deep hole plate clamping seat, a heating assembly, a magnetic suction assembly and a vibration blending assembly, the operation module (9) internally comprises a positioning function, the position is fixed in a stop state every time, and the magnetic suction device can move up and down; the heating of the heating component adopts a fin heating design, the heating of the heating component is uniform, and the heating can be accurately controlled at 0-100 ℃.
4. A biological laboratory manipulator according to claim 1, wherein: 96 channels are arranged in the liquid abandoning and cleaning module (10) and can move into a sample plate, and the liquid abandoning and cleaning module (10) can suck waste liquid under the negative pressure condition.
5. A biological laboratory manipulator according to claim 1, wherein: the liquid adding module (11) is provided with five channel pipelines, the liquid adding module (11) can independently control five reagents through the channel pipelines, and each channel pipeline is provided with twelve liquid spraying nozzles of branch pipes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120801122.6U CN214830378U (en) | 2021-04-19 | 2021-04-19 | Biological experiment operating equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202120801122.6U CN214830378U (en) | 2021-04-19 | 2021-04-19 | Biological experiment operating equipment |
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| CN214830378U true CN214830378U (en) | 2021-11-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276896A (en) * | 2021-12-22 | 2022-04-05 | 成都瀚辰光翼科技有限责任公司 | Automatic nucleic acid extraction system, method and storage medium |
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2021
- 2021-04-19 CN CN202120801122.6U patent/CN214830378U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114276896A (en) * | 2021-12-22 | 2022-04-05 | 成都瀚辰光翼科技有限责任公司 | Automatic nucleic acid extraction system, method and storage medium |
| CN114276896B (en) * | 2021-12-22 | 2023-12-29 | 成都瀚辰光翼科技有限责任公司 | Automatic nucleic acid extraction method and storage medium |
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