CN103789198A - Full automatic instrument for extracting nucleic acids - Google Patents
Full automatic instrument for extracting nucleic acids Download PDFInfo
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- CN103789198A CN103789198A CN201410070377.4A CN201410070377A CN103789198A CN 103789198 A CN103789198 A CN 103789198A CN 201410070377 A CN201410070377 A CN 201410070377A CN 103789198 A CN103789198 A CN 103789198A
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Abstract
The invention discloses a full automatic instrument for extracting nucleic acids, which is capable of fully automating the processes of acquiring sample information, automatically adding samples, automatically loading consumptive materials, automatically monitoring the extraction process, and establishing a PCR reaction system. The full automatic instrument for extracting nucleic acids comprises a working platform, a sample adding system which is mounted on the working platform, a deep-well multiwell plate movement device and a nucleic acid extraction device, wherein a plurality of working positions are arranged on the working platform and comprise a sample area, a reagent area and an extract product storage area. The sample adding system respectively adds the sample and a PCR reaction reagent into the deep-well multiwell plate movement device and the extract product storage area from the sample area and the reagent area respectively, the sample is moved below the nucleic acid extraction device by the deep-well multiwell plate movement device and allowed to perform the nucleic acid extraction reaction, and the extraction products are stored in the extract product storage area. The impact of human factor on experimental result can be effectively reduced, and the purity the extracted nucleic acid and the nucleic acid extraction efficiency can be improved.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, especially relate to a kind of realization from the Full automatic instrument for extracting nucleic acid of the full-automatic operation that sample message collection, sample automatic filling, consumptive material automatic loading, leaching process are monitored automatically, PCR reaction system is set up.
Background technology
Nucleic acid (RNA (ribonucleic acid), thymus nucleic acid DNA) be the elementary cell that represents life entity hereditary feature, detection of nucleic acids is on molecular level, to carry out advanced biological detection, compare traditional morphologic detection, cytology detects, immunology detection etc. have more susceptibility, specificity, without remarkable advantages such as window phases, detection of nucleic acids is recognized as a kind of gold standard method of standard gradually in recent years, be widely used in hepatitis, the serious infectious diseases such as AIDS and SARS, influenza, emerging infectious disease diagnosis and detections such as hand foot mouth disease and be widely used in biology, medical research.Detection of nucleic acids comprises qualitative PCR (Polymerase Chain Reaction, polymerase chain reaction), molecular hybridization, the technology such as real-time fluorescence quantitative PCR (Real time QPCR), the primary key of these nucleic acid detection techniques is to complete the nucleic acid of biological specimen to extract, and therefore extracts efficiently and accurately the nucleic acid-templated prerequisite that becomes follow-up detection of nucleic acids.
Magnetic bead separation and extraction technology use nanotechnology to the surface of superparamagnetic nano particle improve with finishing after, be prepared into superparamagnetism monox nanometer magnetic bead.This magnetic bead can be identified specifically with nucleic acid molecule and efficient combination on micro interface.Utilize the superparamagnetism of monox nanometer microballoon, under the effect of externally-applied magnetic field, can DNA and RNA from blood, animal tissues, food, pathogenic micro-organism equal samples separate, it has the feature such as simple, quick, accurate and has obtained people's generally approval, has become the Main Means of current clinical nucleic acid extraction.
Magnetic bead separation and extraction technology also has some limitations, and its uncertainty of extracting result comes from the impact of experimental implementation and reagent system.Along with the continuous progress of reagent Development Level, the stability of extraction element, consistence also progressively improve, and the principal element that affects nucleic acid extraction result is exactly experimental implementation.Inspection body takes artificial experimental implementation to coordinate the mode of semi-automatic extraction element mostly at present, and extraction element only has single magnetic bead locomotive function, and the pre-treatment of sample, reagent all has operator manually to complete with work such as extracting separating of product.There is individual difference in manual operation, not only affects the accuracy of result, also limited the flux detecting in the time that sample size is various.Can provide automation experiment flow operations, high-throughout nucleic acid extracting instrument to become the important topic that people face.
Summary of the invention
The object of the invention is to overcome the defect of prior art, a kind of Full automatic instrument for extracting nucleic acid is provided, by setting up nucleic acid extraction reaction system and PCR reaction system, realize the full-automatic operation of nucleic acid extraction process, effectively reduce the impact of human factor on experimental result, improved purity and the efficiency of nucleic acid extraction.
For achieving the above object, the present invention proposes following technical scheme: a kind of Full automatic instrument for extracting nucleic acid, comprise workplatform, and be arranged on the sample adding system on described workplatform, deep-well plates running gear, nucleic acid-extracting apparatus, a plurality of work stations are also set on described workplatform, described work station comprises sample area, reagent area and extraction product storage area, described sample adding system is given respectively described deep-well plates running gear and extracts product storage area load sample and PCR reaction reagent from sample area and reagent area, sample is transplanted on described nucleic acid-extracting apparatus below by described deep-well plates running gear, carry out the nucleic acid extraction reaction of sample, the extraction product that reaction forms leaves described extraction product storage area in.
Preferably, described sample adding system comprises transverse moving device, the longitudinal motion device vertical with described transverse moving device and is arranged on the sample adding device on described longitudinal motion device, and described transverse moving device coordinates with described longitudinal motion device and drives described sample adding device to sample to relevant work station.
Described transverse moving device comprises horizontal drive motor, laterally drives slide rail and laterally drive slide block, described longitudinal motion device is connected with described horizontal driving slide block, and described longitudinal motion device carries out traverse motion with described sample adding device along described horizontal driving slide rail under the driving of described horizontal drive motor.
Described longitudinal motion device comprises vertical drive motor, longitudinally drives slide rail and longitudinally drive slide block, described sample adding device is connected with described longitudinal driving slide block, and described longitudinal driving slide block carries out longitudinal to-and-fro movement with described sample adding device along described longitudinal driving slide rail under the driving of described vertical drive motor.
Sample loading gun vertical drive mechanism and sample loading gun switch that described sample adding device comprises automatic sampling gun, is connected with described automatic oil gun, sample loading gun vertical drive mechanism drives described automatic sampling gun to move up and down described in described sample loading gun on-off control.
The end of described automatic sampling gun is provided with sliding sleeve, for unloading used disposable rifle head.
Described sample loading gun vertical drive mechanism comprises pressurization rifle drive-motor, the transmission rig that is arranged on the buffer gear on described automatic sampling gun and is connected with described pressurization rifle drive-motor, described pressurization rifle drive-motor drives described transmission rig to move up and down with described automatic sampling gun.
Described sample area comprises sample loading module and the bar code scan module being connected with described sample loading module, described sample loading module comprises sample loading base, is arranged on a plurality of sample pipe supports on described sample loading base and inserts the sample tube being loaded on described sample pipe support, described bar code scan module scans the sample tube on described sample pipe support one by one, records the information of each sample tube.
Described bar code scan module comprises sample rack haulage gear, be arranged on the barcode scanner of described sample rack haulage gear one side, and with the vertical translational drive mechanism being connected of described sample rack haulage gear, described sample rack haulage gear drags whole piece sample pipe support and moves back and forth along the direction parallel with described barcode scanner, described translational drive mechanism promotes described sample rack haulage gear and moves to the sample pipe support place of respective scanned, one by one sample tube is scanned.
Described work station also comprises rifle head storage area and waste recovery zone, on described rifle head storage area, be mounted with the disposable rifle head that application of sample is used, described waste recovery zone comprises waste liquid recovery apparatus and discarded rifle head retrieving arrangement, is respectively used to collect the waste liquid and the used rifle head that in nucleic acid extraction process, produce.
Described reagent area comprises reagent preservation module and mixes in advance cup position, described reagent is preserved module and is comprised low temperature inner core, is arranged on temperature control unit on described low temperature inner core, covers insulation cover and a plurality of reagent bottle on described low temperature inner core, and described reagent bottle leaves in-core in described low temperature in.
Each described reagent bottle is integral structure, comprise Reagent Tube support and be encapsulated in a plurality of Reagent Tubes on described Reagent Tube support, the in-built reagent that carries different components of different described Reagent Tubes, when use, is placed into in-core in low temperature by reagent bottle entirety and carries out cryopreservation.
On described insulation cover, corresponding reagent bottle is provided with plural number row well, and described automatic sampling gun is drawn reagent through described well from described reagent bottle.
Deep-well plates locating device that described deep-well plates running gear comprises deep-well plates actuator, be flexibly connected with described deep-well plates actuator, be arranged on the deep-well plates on described deep-well plates locating device, described deep-well plates actuator drags described deep-well plates locating device entirety and moves to described nucleic acid-extracting apparatus below.
In described deep-well plates, insert and be loaded with plural number stirring cover, on the upper surface of described stirring cover, be provided with hook, be convenient to stir the unloading of cover running gear to stirring cover.
On described extraction product storage area, be placed with microwell plate, on described microwell plate, be provided with a plurality of taper holes, for loading the product after extraction.
On described workplatform, also comprise consumptive material drawer, described rifle head storage area, reagent area, deep-well plates running gear, extraction product storage area and waste recovery zone are installed on described consumptive material drawer, make these work stations with the pull-out of consumptive material drawer entirety, to be convenient to sample, reagent, the first-class timely replacing of rifle.
The stirring cover drive-motor that described nucleic acid-extracting apparatus comprises bar magnet running gear, is connected with described bar magnet running gear and drives its bar magnet drive-motor moving up and down, stirs cover running gear, is connected and drives it to move up and down with described stirring cover running gear.
On described workplatform, also comprise aerosol proofing unit, described aerosol proofing unit comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, described discharge cell electric discharge ionized gas molecule generates gaseous ion, described gaseous ion is combined with aerosol particle, the too much gaseous ion that ionization produces is removed in described ion deposition unit, described conduction filter membrane intercepts charged aerosol particle, described detecting unit detects the quantity of electric charge of the charged aerosol particle of described intercepting, obtain concentration and the particle diameter information of described aerosol particle, in the time that the concentration of aerosol particle reaches certain value, just report to the police in time, accuracy and the validity of nucleic acid extraction result are guaranteed.
Compared with prior art, beneficial effect of the present invention is:
1, can complete automatically that sample message collection, sample automatic filling, consumptive material automatic loading, leaching process in nucleic acid extraction process monitored automatically, PCR reaction system is set up, avoid the interference of manual operation to test-results in leaching process, strengthen validity and the accuracy of extracting result, improved the efficiency of nucleic acid extraction.
2, the present invention takes deep-well plates running gear entirety auto-translating is to the scheme of extracting district, compared with the technology that shifts deep-well plates with existing employing mechanical arm, more succinct reliable.
3, the present invention arranges reagent and preserves module in reagent area, has the function of low-temperature storage reagent, has extended the duration of service of reagent, has guaranteed the functional quality of reagent.
4, the reagent bottle that the present invention the adopts encapsulating structure that is integral type, simple in structure, be convenient to processing.
5, nucleic acid extracting reagent of the present invention adopts pre-packaged form to preserve, and the mode that existing full-automatic nucleic acid extraction equipment all adopts complete machine to be annotated by syringe imbibition, the solution of the present invention has effectively been simplified the complexity of reagent filling operation, has improved the reliability of complete machine and the accuracy of reagent filling result.
6, the present invention adds the spillage of aerosol proofing unit for timely Monitoring equipment working process reaction solution gas molecule, in the time reaching finite concentration, can send warning, effective monitoring nucleic acid extraction process, the confidence level of nucleic acid extraction result is further enhanced.
Accompanying drawing explanation
Fig. 1 is the one-piece construction schematic diagram of Full automatic instrument for extracting nucleic acid of the present invention;
Fig. 2 is the schematic layout pattern of workplatform of the present invention;
Fig. 3 is the structural representation of sample adding system of the present invention;
Fig. 4 is the structural representation of sample adding device in Fig. 3;
Fig. 5 is the structural representation of sample area of the present invention;
Fig. 6 is the structural representation of reagent area of the present invention;
Fig. 7 is the structural representation of waste of the present invention recovery zone;
Fig. 8 is the structural representation of deep-well plates running gear of the present invention;
Fig. 9 is the structural representation of nucleic acid-extracting apparatus of the present invention;
Figure 10 is that the present invention stirs the structural representation that cover captures.
Embodiment
Below in conjunction with accompanying drawing of the present invention, the technical scheme of the embodiment of the present invention is carried out to clear, complete description.
Disclosed a kind of Full automatic instrument for extracting nucleic acid, for realizing the fully automated of nucleic acid extraction process and PCR reaction, described extraction apparatus comprises operative body and controls computer (not shown), described control computer, for carrying out the acquisition process etc. of sample data information, is realized the operation of controlling and monitoring described operative body.In conjunction with Fig. 1, shown in Fig. 2, described operative body comprises casing assembly, and be arranged on the workplatform 1 in described casing assembly, sample adding system 2, deep-well plates running gear 16, nucleic acid-extracting apparatus 18, aerosol proofing unit 4, on described workplatform 1, be also provided with a plurality of work stations, described work station comprises sample area 11, reagent area 12, rifle head storage area (13, 14), waste recovery zone 15 and extraction product storage area 17, these work stations are for loading the required sample reagent of nucleic acid extraction process, PCR reaction reagent, disposable rifle is first-class.
Described casing assembly is for playing protective effect to each several part in described operative body, as shown in Figure 1, described casing assembly comprises frame 38 and is encapsulated in the shield cap outside described frame 38, described shield cap comprises main shield cap 31, side shield cap (32, 33), drawer panel (34, 35), sample area panel 36 and rear defence guard shield 37, described main shield cap 31, side shield cap (32, 33) and rear defence guard shield (34, 35) other faces except bottom surface in frame 38 are encapsulated, described drawer panel (34, 35) be connected with described work station, described sample area panel 36 is arranged on described main shield cap 31, be used for encapsulating described sample area 11, the structure design of certain described casing assembly is not limited to the structure of above-mentioned introduction, as long as can realize the encapsulation of operative body and the integral drawing of work station.
Described sample adding system 2 is for realizing the automatic delivery of sample, PCR reaction reagent, extraction product etc.Shown in Fig. 2 and Fig. 3, described sample adding system 2 comprises transverse moving device 21, longitudinal motion device 22 and sample adding device 23, described longitudinal motion device 22 and described vertical connection of transverse moving device 21, described sample adding device 23 is arranged on described longitudinal motion device 22, and described transverse moving device 21 coordinates the described sample adding device 23 of control to run to each work station place with described longitudinal motion device 22.
Shown in Fig. 2 and Fig. 3, described transverse moving device 21 comprises horizontal driving slide rail 212, and the horizontal drive motor 211 being connected with described horizontal driving slide rail 212 and the horizontal slide block 213 that drives, described horizontal driving slide block 213 is connected with described longitudinal motion device 22, and described horizontal drive motor 211 drives described horizontal driving slide block 213 to drive described sample adding device 23 laterally to slide along described horizontal driving slide rail 212.Described longitudinal motion device 22 comprises vertical drive motor 221, longitudinally drives slide rail 222 and longitudinally drive slide block 223, described sample adding device 23 is connected with described longitudinal driving slide block 223, and described vertical drive motor 221 drives described longitudinal driving slide block 223 to drive described sample adding device 23 to carry out wiping action along described longitudinal driving slide rail 223.
On described horizontal driving slide rail 212 and described longitudinal driving slide rail 222, be provided with screw mandrel (not shown), vertical or horizontal drive-motor drives its corresponding motion that drives the screw mandrel realization driving slide block on slide rail.
As shown in Figure 4, described sample adding device 23 comprises automatic sampling gun 231, sample loading gun vertical drive mechanism 232 and sample loading gun switch 237, described automatic sampling gun 231 inside are provided with vacuum unit (not shown), described automatic sampling gun 231 is realized the suction of liquid by described vacuum unit, the lower end of described automatic sampling gun 231 is provided with sliding sleeve 233, and described sliding sleeve 233 is for realizing the automatic drawing unloading of rifle head.
Described sample loading gun switch 237 is controlled described sample loading gun vertical drive mechanism 232 and is driven described automatic sampling gun 231 to move up and down.Take the transverse moving device 21 in sample adding system 2 as X-axis, longitudinal motion device 22 sets up system of coordinates as Y-axis and sample adding device 23 as Y-axis, described transverse moving device 21, longitudinal motion device 22 and sample adding device 23 drive respectively described automatic sampling gun 231 to move up in X, Y, the Z side of system of coordinates.
Described sample area 11 is for load sample reagent and realize the collection of sample information, and described sample reagent can adopt blood or tissue juice sample.As shown in Figure 5, described sample area 11 comprises sample loading module and bar code scan module, described sample loading module comprises a plurality of sample pipe supports 111, sample tube 112 and sample loading base 113, described sample pipe support 111 is movably connected on described sample loading base 113, on described sample pipe support 111, plurality of sample pore is set, described sample tube is placed in described sample pore.Sample tube 112 in the embodiment of the present invention adopts Φ 13 × 100mm standard heparin tube, and the equal label in its side, for the scanning of bar code scan module.
Described bar code scan module comprises sample barcode scanner 114, sample rack haulage gear 115, translational drive mechanism 116, described sample barcode scanner 114 is arranged on a side of described sample rack haulage gear 115, described sample rack haulage gear 115 is connected with described sample pipe support 111, being used for driving described sample pipe support 111 to be parallel to described sample barcode scanner 114 moves back and forth, described translational drive mechanism 116 is connected with described sample rack haulage gear 115, for promoting sample pipe support 111 places of described sample rack haulage gear 115 to respective scanned, one by one sample tube 112 is scanned.
The cooperatively interacted autoscan of sample reagent of described sample loading module and bar code scan module, particularly, when bar code scan, described sample rack haulage gear 115 drags 111 to-and-fro movements of sample pipe support, sample barcode scanner 114 is recorded the bar code information of each sample tube 112, after first sample pipe support 111 has scanned, translational drive mechanism 116 promotes sample rack haulage gear 115 integral translations to a second sample pipe support 111 and scans, by that analogy until the last item sample pipe support 111 all completes scanning.
Required reagent is reacted for loading PCR in described reagent area 12, as shown in Figure 6, described reagent area 12 comprises reagent preservation module 121 and mixes in advance cup position 122, described reagent is preserved module 121 for cryopreservation PCR reaction reagent, it comprises temperature control unit 123, low temperature inner core 124 and insulation cover 125, described temperature control unit 123 is arranged on described low temperature inner core 124, for regulating the temperature of described low temperature inner core 124, make it in preserving under the suitable low temperature environment of reagent place, on described low temperature inner core 124, be provided with a plurality of reagent bottles chamber, in described reagent bottle chamber, be mounted with the reagent bottle 126 that holds PCR reaction reagent, described insulation cover 125 covers the upper end of described heat insulation inner core 124, described low temperature inner core 124 is played to certain heat insulation effect, on described insulation cover 125, corresponding reagent bottle 126 is provided with plural number row well 127, described automatic sampling gun 231 is drawn reagent through described well 237 from described reagent bottle 126.
Unlike the prior art, reagent bottle 126 in the present invention is designed to integral structure, comprise Reagent Tube support 128 and be encapsulated in a plurality of Reagent Tubes 129 on described Reagent Tube support 128, the in-built PCR reaction reagent that carries different components of different described Reagent Tubes 129.Reagent bottle 126 in the embodiment of the present invention is that Dispoable medical polypropylene material is injection molded, in-built described PCR reaction reagent mainly comprises two kinds: reaction internal reference reagent and PCR reaction premix reagent, when use, reagent bottle 126 entirety are put into low temperature inner core 124 cryopreservation.
Described rifle head storage area extracts required disposable rifle head for depositing, according to actual needs, multiple rifle head storage areas can be set on described workplatform 1, the present embodiment illustrates the structure of workplatform 1 in the present invention so that two rifle head storage areas to be set as example, in conjunction with Fig. 2, shown in Fig. 7, described rifle head storage area comprises the first rifle head storage area 13 and the second rifle head storage area 14, for the ease of changing in time rifle head, described the first rifle head storage area 13 is installed on a consumptive material drawer 141 with reagent area 12, described consumptive material drawer 141 is realized the integral drawing of described rifle head storage area.
The waste that described waste recovery zone produces for reclaiming nucleic acid extraction process, shown in Fig. 2 and Fig. 7, described waste recovery zone 15 comprises waste liquid recovery apparatus 151 and discarded rifle head retrieving arrangement 152, be respectively used to collect the waste liquid and the used disposable rifle head that in nucleic acid extraction process, produce, two devices are all recycled to waste in the container (not shown) of workplatform below.
Described extraction product storage area 17 is for depositing the product after extraction, shown in Fig. 1 and Fig. 7, on described extraction product storage area 17, be placed with microwell plate 171, microwell plate 171 in the embodiment of the present invention adopts Dispoable medical polypropylene material injection molded, form (8 row × 12 row) by 96 taper holes, volume has 0.1ml, two kinds of specifications of 0.2ml available.
Described deep-well plates running gear 16 is for load sample and drive sample to arrive the below of described nucleic acid-extracting apparatus 18, carries out the automatic extraction of nucleic acid.As shown in Figure 8, described deep-well plates running gear 16 comprises deep-well plates actuator 161, deep-well plates actuator 162 and deep-well plates 164, described deep-well plates 164 is positioned on described deep-well plates actuator 161, be used for placing stirring and overlap 163, described deep-well plates actuator 162 is connected with described deep-well plates actuator 161, be used for dragging described deep-well plates actuator 161 moves back and forth between starting position and described nucleic acid-extracting apparatus 18 lower positions, when use, after operator will stir in cover 163 insertion deep-well plates 164, deep-well plates 164 is arranged on deep-well plates locating structure 161, described deep-well plates actuator 162 can drag deep-well plates locating structure 161 to nucleic acid-extracting apparatus 18 belows and carry out the abstraction reaction of nucleic acid.
On described deep-well plates locating structure 161, heating unit (not shown) is also housed, can heats described deep-well plates 164.
Deep-well plates 164 in the embodiment of the present invention adopts Dispoable medical polypropylene material injection molded, forms (8 row × 12 row), and at the bottom of hole, be circular arc camber by 96 square holes.
Described nucleic acid-extracting apparatus 18 is for carrying out nucleic acid extraction reaction process, described process comprises the cracking of sample, washing and wash-out, as shown in Figure 9, described nucleic acid-extracting apparatus 18 comprises bar magnet running gear 181, stir cover running gear 182, bar magnet drive-motor 183 and stirring cover drive-motor 184, described bar magnet drive-motor 183 is connected with described bar magnet running gear 181 and drives described bar magnet fitness machine 181 to pump, described stirring cover drive-motor 184 is connected with described stirring cover running gear 182, and drive described stirring cover running gear 182 to pump.
Unlike the prior art, the present invention has added aerosol proofing unit 4 on instrument, utilize ionized gas molecule to make electric charge on aerosol particle band, between the average band electric weight of aerosol particle and particle diameter, there is definite exponential relationship, just can obtain the concentration of particle and the principle of particle diameter information by the average band electric weight of surveying particle, the spillage of reaction solution gas molecule in detecting instrument working process in time, in the time reaching finite concentration, warning be can send, the safety in utilization of instrument and the accuracy of experimental result improved.Described aerosol proofing unit 4 comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, its specific works principle is: described discharge cell electric discharge ionized gas molecule, the gaseous ion that ionization is generated is combined with aerosol particle, thereby makes aerosol particle charged; After aerosol particle is charged, through ion deposition unit ion deposition, remove the too much gaseous ion producing due to ionization, avoid them to exert an influence to ensuing measurement; Finally charged aerosol particle is dammed to described conduction filter membrane, detect the quantity of electric charge on aerosol particle by detecting unit, draw concentration and the particle diameter information of aerosol particle through data inversion.
The specific works flow process of Full automatic instrument for extracting nucleic acid of the present invention is as follows:
Before instrument work, first will complete following preparation work: operator packs stirring cover 163 into the specific bit of deep-well plates 164, pull-out consumptive material drawer 141, is arranged on deep-well plates 164 on deep-well plates locating structure 161; Reagent bottle 126 is arranged in the low temperature inner core 124 of reagent area 12, simultaneously packs standard 20ml cryopreservation tube into for reagent premix mixing in advance cup position 122; Pack one by one sample pipe support 111 into sample area 11; The disposable rifle head of the first rifle head storage area 13 and the second rifle head storage area 14 is supplemented complete; To be placed on and extract product storage area 17 without the microwell plate 171 using.
After preparation work completes, start the following flow process of extracting: first sample pipe support 111 is scanned one by one, record the bar code information of each sample tube 112; Simultaneously, described deep-well plates running gear 16 integral translations are to described nucleic acid-extracting apparatus 18 belows, as shown in figure 10, after described stirring cover running gear 182 moves downward stirring cover is taken out from deep-well plates 164, described deep-well plates running gear 16 moves to starting position.
Secondly, automatic sampling gun 231 is under the control of transverse moving device 21, longitudinal motion device 22, sample adding device 23, moving to the first rifle head storage area 13 loads after disposable rifle head, move to again sample area 11 and draw sample, afterwards sample is delivered to deep-well plates 164 stock layouts bases, finally move to discarded rifle head retrieving arrangement 152, by disposable rifle head unloading, automatic sampling gun 231 repeats said process, until sample is all added into deep-well plates 164.
Then, described deep-well plates running gear 16 moves to nucleic acid-extracting apparatus 18 belows, bar magnet running gear 181 pumps, carry out the automatic extraction of nucleic acid, in nucleic acid extraction, described automatic sampling gun 231 moves to the first rifle head storage area 13 and loads disposable rifle head, move to again reagent area 12 and inhale reagent, then move to extraction product storage area 17 and arrange reagent, complete after reagent filling, automatic sampling gun 231 motion unloading rifle heads are to discarding rifle head retrieving arrangement 152.
Again then, the bar magnet running gear 181 on described nucleic acid-extracting apparatus 18 and 182 relative movement of stirring cover running gear, be offloaded to stirring cover 163 in deep-well plates, and after stirring cover 163 has unloaded, described deep-well plates running gear 16 returns to starting position.
Afterwards, described automatic sampling gun 231 moves to the second rifle head storage area 14 and loads disposable rifle head, then moves to deep-well plates 164 suction extraction products, then moves to and extracts product storage area 17 row's extraction products, finally move to discarded rifle head retrieving arrangement 152, by disposable rifle head unloading.
Finally, described automatic sampling gun 231 moves to the first rifle head storage area 13 and loads disposable rifle head, move to again deep-well plates 164 and inhale waste reaction solution, then move to waste liquid recovery apparatus 151 removal waste fluids, after repetition aforesaid operations to the waste liquid in all deep-well plates 164 sucks, move to discarded rifle head retrieving arrangement 152, the sliding sleeve 233 of automatic sampling gun 231 ends unloads disposable rifle head again, and whole nucleic acid extraction flow process finishes.
Technology contents of the present invention and technical characterictic have disclosed as above; but those of ordinary skill in the art still may be based on teaching of the present invention and announcements and are done all replacement and modifications that does not deviate from spirit of the present invention; therefore; protection domain of the present invention should be not limited to the content that embodiment discloses; and should comprise various do not deviate from replacement of the present invention and modifications, and contained by present patent application claim.
Claims (10)
1. a Full automatic instrument for extracting nucleic acid, it is characterized in that, comprise workplatform, and be arranged on the sample adding system on described workplatform, deep-well plates running gear, nucleic acid-extracting apparatus, a plurality of work stations are also set on described workplatform, described work station comprises sample area, reagent area and extraction product storage area, described sample adding system is given respectively described deep-well plates running gear and extracts product storage area load sample and PCR reaction reagent from sample area and reagent area, sample is transplanted on described nucleic acid-extracting apparatus below by described deep-well plates running gear, carry out the nucleic acid extraction reaction of sample, the extraction product that reaction forms leaves described extraction product storage area in.
2. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described reagent area comprises reagent preservation module and mixes in advance cup position, described reagent is preserved module and is comprised low temperature inner core, is arranged on temperature control unit on described low temperature inner core, covers insulation cover and a plurality of reagent bottle on described low temperature inner core, and described reagent bottle leaves in-core in described low temperature in.
3. Full automatic instrument for extracting nucleic acid according to claim 2, it is characterized in that, each described reagent bottle is integral structure, comprises Reagent Tube support and is encapsulated in a plurality of Reagent Tubes on described Reagent Tube support, the in-built reagent that carries different components of different described Reagent Tubes.
4. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, deep-well plates locating device that described deep-well plates running gear comprises deep-well plates actuator, be flexibly connected with described deep-well plates actuator, be arranged on the deep-well plates on described deep-well plates locating device, described deep-well plates actuator drags described deep-well plates locating device entirety and moves to described nucleic acid-extracting apparatus below.
5. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, on described workplatform, also comprise aerosol proofing unit, described aerosol proofing unit comprises discharge cell, ion deposition unit, conduction filter membrane and detecting unit, described discharge cell electric discharge ionized gas molecule generates gaseous ion, described gaseous ion is combined with aerosol particle, the too much gaseous ion that ionization produces is removed in described ion deposition unit, described conduction filter membrane intercepts charged aerosol particle, described detecting unit detects the quantity of electric charge of the charged aerosol particle of described intercepting, obtain concentration and the particle diameter information of described aerosol particle.
6. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described work station also comprises rifle head storage area and waste recovery zone, on described rifle head storage area, be mounted with the disposable rifle head that application of sample is used, described waste recovery zone comprises waste liquid recovery apparatus and discarded rifle head retrieving arrangement, is respectively used to collect the waste liquid and the used rifle head that in nucleic acid extraction process, produce.
7. Full automatic instrument for extracting nucleic acid according to claim 6, it is characterized in that, on described workplatform, also comprise consumptive material drawer, described rifle head storage area, reagent area, deep-well plates running gear, extraction product storage area and waste recovery zone are installed on described consumptive material drawer.
8. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described sample adding system comprises transverse moving device, the longitudinal motion device vertical with described transverse moving device and is arranged on the sample adding device on described longitudinal motion device, and described transverse moving device coordinates with described longitudinal motion device and drives described sample adding device to sample to relevant work station.
9. Full automatic instrument for extracting nucleic acid according to claim 1, it is characterized in that, described sample area comprises sample loading module and the bar code scan module being connected with described sample loading module, described sample loading module comprises sample loading base, is arranged on a plurality of sample pipe supports on described sample loading base and inserts the sample tube being loaded on described sample pipe support, and described bar code scan module scans the sample tube on described sample pipe support one by one.
10. Full automatic instrument for extracting nucleic acid according to claim 1, is characterized in that, on described extraction product storage area, is placed with microwell plate, is provided with multiple taper holes on described microwell plate.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101143884A (en) * | 2007-10-15 | 2008-03-19 | 吉林大学 | Nucleic acid automatic extracting instrument |
| WO2009054870A2 (en) * | 2007-07-13 | 2009-04-30 | Handylab, Inc. | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN101538612A (en) * | 2009-04-16 | 2009-09-23 | 上海科华生物工程股份有限公司 | Integrated blood nucleic acid screening platform |
| CN101747397A (en) * | 2008-11-28 | 2010-06-23 | 霍夫曼-拉罗奇有限公司 | System and method for the automated extraction of nucleic acids |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
-
2014
- 2014-02-27 CN CN201410070377.4A patent/CN103789198B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009054870A2 (en) * | 2007-07-13 | 2009-04-30 | Handylab, Inc. | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN101143884A (en) * | 2007-10-15 | 2008-03-19 | 吉林大学 | Nucleic acid automatic extracting instrument |
| CN101747397A (en) * | 2008-11-28 | 2010-06-23 | 霍夫曼-拉罗奇有限公司 | System and method for the automated extraction of nucleic acids |
| CN101538612A (en) * | 2009-04-16 | 2009-09-23 | 上海科华生物工程股份有限公司 | Integrated blood nucleic acid screening platform |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
| CN103305412A (en) * | 2013-05-31 | 2013-09-18 | 广州安必平自动化检测设备有限公司 | Full-automatic nucleic acid extraction machine |
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