CN105838582A - Automatic sample separating component for mycoplasma identification and drug sensitivity analysis - Google Patents

Automatic sample separating component for mycoplasma identification and drug sensitivity analysis Download PDF

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Publication number
CN105838582A
CN105838582A CN201610352595.6A CN201610352595A CN105838582A CN 105838582 A CN105838582 A CN 105838582A CN 201610352595 A CN201610352595 A CN 201610352595A CN 105838582 A CN105838582 A CN 105838582A
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sample
lath
support
mycoplasma
point
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CN105838582B (en
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陈彩霞
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JIANGMEN KAILIN TRADE CO Ltd
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JIANGMEN KAILIN TRADE CO Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18Testing for antimicrobial activity of a material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Abstract

The invention discloses an automatic sample separating component for mycoplasma identification and drug sensitivity analysis. The automatic sample separating component comprises a sampling moving platform, a consumable bracket, a sample bracket, a blank lath bracket, a sample separating lath bracket and a waste collecting bin, wherein the sampling moving platform can move among the consumable bracket, the sample bracket, the blank lath bracket, the sample separating lath bracket and the waste collecting bin, so as to perform automatic sample separating. The automatic sample separating component replace manual sample separating operation in traditional operation, greatly improves the sample separating efficiency, realizes the automatic operation of sample separating in mycoplasma identification and drug sensitivity analysis, reduces manpower and operation time required by mycoplasma identification and drug sensitivity analysis, can realize a large amount of sample identification and drug sensitivity analysis through low cost, and meets the requirements of hospitals or relevant research institutes.

Description

A kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility
Technical field
The present invention relates to mycoplasma and identify field, especially relate to a kind of qualification for mycoplasma and point sample assembly automatically of Analysis of Drug Susceptibility.
Background technology
The multiple states such as mycoplasma is the minimum microorganism of extracellular existence, is the class prokaryotic microorganism that lacks cell wall, and size is typically between 0.3~0.5um, in height pleomorphism, has spherical a, rod, thread, branched.It is different from cell, and also different from virus, harm of a great variety, widely distributed, that cause is quite big, relates to multiple fields such as people, animal, plant and insecticide, brings adverse effect to human health and research work.Mycoplasma is identified and the operation of susceptibility detection at present, it usually needs being accomplished manually, its main operating process is: inoculation, is joined in nutrient broth by swab of drawing materials by detected sample;Mixing, makes tested sample fully mix with nutrient broth;Point sample also seals paraffin wax, will inoculate specimen and the nutrient broth that fully mixes joins in the hole of qualification and medicine sensitive detecting plate, then seals up paraffin wax;Hatch cultivation, by point good sample and seal the detection plate of paraffin wax and put into incubator and hatch certain time;Sentence read result, changes according to the color of each aperture and judges that tested sample is negative or positive, and the sensitivity to various antibiotic.But manually-operated point of sample efficiency comparison is low, needing relatively more operators to operate, especially in carrying out substantial amounts of sample qualification and Analysis of Drug Susceptibility, time and the manpower of needs are the most, mycoplasma is identified and the cost of Analysis of Drug Susceptibility remains high, it is difficult to meet the demand of hospital.
Summary of the invention
In order to overcome the deficiencies in the prior art, the present invention provides a kind of and identifies for mycoplasma and point sample assembly automatically of Analysis of Drug Susceptibility, and it has the function of point sample automatically, and point sample efficiency is high, point sample used time is short, low cost.
The technical solution adopted in the present invention is mainly: a kind of identify for mycoplasma and point sample assembly automatically of Analysis of Drug Susceptibility, including sampling mobile platform, consumptive material support, sample arm, blank plates support, point model bar support and waste collection storehouse, described consumptive material support is placed with tip head and liquid wax, described sample arm is placed with the container equipped with sample solution, described sampling mobile platform can at consumptive material support, screed support, blank plates support, mobile point between model bar support and waste collection storehouse automatically divide sample.
Improvement as technique scheme, described sampling mobile platform includes sample charging mechanism, Sampling Machine mechanical arm and carrying handgrip, described Sampling Machine mechanical arm is driven by motor and is slidably mounted on the sampling slide rail that point sample assembly is provided with automatically, Sampling Machine mechanical arm is provided with the horizontal slide rail of mechanical arm and the vertical slide rail of mechanical arm, described sample charging mechanism and carrying handgrip and is driven by corresponding motor and be slidably mounted at respectively on the horizontal slide rail of mechanical arm and the vertical slide rail of mechanical arm.
Further improvement as technique scheme, described sample charging mechanism is provided with sample-adding body frame, it is provided with liquid feeding pin on described sample-adding body frame, automatic pipettor and bar code scan instrument, described automatic pipettor can load tip head automatically at consumptive material support, tip head is unloaded in waste collection storehouse, sample solution is sucked in the container of sample arm, and the sample solution of suction is joined each Kong Weizhong of each lath on blank plates support, described liquid feeding pin is for carrying out sealing wax to the lath filling liquid wax being loaded, described bar code scan instrument for being scanned reading to lath information.
Further, it is provided with two on described sample-adding body frame and is loaded vertical slide rail, described liquid feeding pin and bar code scan instrument driven by motor be slidably mounted at wherein one be loaded on vertical slide rail, described automatic pipettor is driven by motor and is slidably mounted at another and is loaded on vertical slide rail.
Further, described blank plates support is identical with the structure of point model bar support, all include lath stent bearing plank, multiple lath support it is stacked with on described lath stent bearing plank, uniform on described lath support arranging multiple lath mounting groove adaptive with lath, described lath is installed on lath mounting groove.
Further, described lath one end is provided with lath bar code district and detection hole, and the lath other end is vertically disposed with first row detection hole and second row detection hole.
Further, described carrying handgrip is provided with two, the locating dowel of protrusion it is provided with inside carrying handgrip, it is formed with the guide edge making way for locating dowel along indent under described lath support two ends, described guide edge is provided with the locating slot suitable with locating dowel, and described carrying handgrip can slide along guide edge under machinery of sampling arm straps is dynamic and snap in locating slot by locating dowel and moved on point model bar support by the lath support completing point sample on blank plates support.
Further, described automatic point of sample assembly is additionally provided with a plurality of guide rail, and described consumptive material support, screed support, blank plates support and a point model bar support can be slidably mounted on corresponding guide rail respectively.
The invention has the beneficial effects as follows:
The a kind of of the present invention identifies for mycoplasma and point sample assembly automatically of Analysis of Drug Susceptibility, it is provided by mobile platform of sampling, sampling mobile platform can be at consumptive material support, screed support, blank plates support, divide to move between model bar support and waste collection storehouse and automatically divide sample, instead of the people's work point sample operation in traditional operation, substantially increase the efficiency of point sample, achieve mycoplasma to identify and the automation mechanized operation of point sample in Analysis of Drug Susceptibility, reduce required manpower and operating time in mycoplasma qualification and Analysis of Drug Susceptibility, substantial amounts of sample can be realized with relatively low cost to identify and Analysis of Drug Susceptibility, meet the demand of hospital or related research institutes.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the present invention;
Fig. 2 is the structural representation of sampling mobile platform;
The structural representation of Fig. 3 sample charging mechanism;
Fig. 4 is a point structural representation for model bar support;
Fig. 5 is the connection diagram of lath support and lath;
Fig. 6 is the structural representation of lath.
Detailed description of the invention
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
nullReferring to figs. 1 through Fig. 6,The a kind of of the present invention identifies for mycoplasma and point sample assembly automatically of Analysis of Drug Susceptibility,Including sampling mobile platform 1、Consumptive material support 2、Sample arm 3、Blank plates support 4、Divide model bar support 5 and waste collection storehouse 6,Consumptive material support 2 is placed with tip head and liquid wax,Sample arm 3 is placed with the container equipped with sample solution,Lath 43 all it is placed with on blank plates support 4 and point model bar support 5,Sampling mobile platform 1 has the degree of freedom in three vertical direction,It can be at consumptive material support 2、Screed support 3、Blank plates support 4、Divide to move between model bar support 5 and waste collection storehouse 6 and automatically divide sample,Thus instead of the people's work point sample operation in traditional operation,Substantially increase the efficiency of point sample,Achieve mycoplasma to identify and the automation mechanized operation of point sample in Analysis of Drug Susceptibility,Reduce required manpower and operating time in mycoplasma qualification and Analysis of Drug Susceptibility,Substantial amounts of sample can be realized with relatively low cost to identify and Analysis of Drug Susceptibility,Meet the demand of hospital or related research institutes.
Concrete, sampling mobile platform 1 includes sample charging mechanism 11, Sampling Machine mechanical arm 12 and carrying handgrip 13, automatically a point sample assembly is provided with sampling slide rail 14, sampling slide rail 14 can be provided with two, improve the stationarity slided, Sampling Machine mechanical arm 12 is driven by motor and is slidably mounted on sampling slide rail 14, Sampling Machine mechanical arm 12 is provided with the horizontal slide rail of mechanical arm 15 and the vertical slide rail of mechanical arm 16, described sample charging mechanism 11 and carrying handgrip 13 and is driven by corresponding motor and be slidably mounted at respectively on the horizontal slide rail of mechanical arm 15 and the vertical slide rail of mechanical arm 16.In the present invention, above-mentioned motor is provided with tape handler, Sampling Machine mechanical arm 12, sample charging mechanism 11 and carrying handgrip 13 be all by connect with the belt of tape handler and slidably on corresponding slide rail, certainly, above-mentioned parts can also use Chain conveyer or other kinds of drive, and the present invention is not limited to this.
Sample charging mechanism 11 is provided with sample-adding body frame 111, it is provided with liquid feeding pin 112 on sample-adding body frame 111, automatic pipettor 113 and bar code scan instrument 114, automatic pipettor 113 can load tip head automatically at consumptive material support 2, tip head is unloaded in waste collection storehouse 6, sample solution is sucked in the container of sample arm 3, and the sample solution of suction is joined each Kong Weizhong of each lath 41 on blank plates support 4, described liquid feeding pin 112 carries out sealing wax for the lath 41 being loaded is filled liquid wax, bar code scan instrument 114 for being scanned reading to lath 41 information, thus sample information is associated with this lath 41.
For making liquid feeding pin 112, automatic pipettor 113 and bar code scan instrument 114 can move easily vertical, it is provided with two on sample-adding body frame 111 and is loaded vertical slide rail 115, described liquid feeding pin 112 and bar code scan instrument 114 driven by motor be slidably mounted at wherein one be loaded on vertical slide rail 115, described automatic pipettor 113 is driven by motor and is slidably mounted at another and is loaded on vertical slide rail 115.In the present invention, above-mentioned motor is provided with tape handler, automatic pipettor 113, liquid feeding pin 112 and bar code scan instrument 114 be all by connect with the belt of tape handler and slidably on corresponding slide rail, certainly, above-mentioned parts can also use Chain conveyer or other kinds of drive, and the present invention is not limited to this.
In the present invention, blank plates support 4 is identical with the structure of point model bar support 5, all include lath stent bearing plank 41, multiple lath support 42 it is stacked with on lath stent bearing plank 41, on lath support 42 uniform arrange multiple with lath 43 adaptation lath mounting groove 421, lath 43 is installed on lath mounting groove 421, and lath 43 one end is provided with lath bar code district 431 and detection hole 432, and lath 43 other end is vertically disposed with first row detection hole 433 and second row detection hole 434.
Carrying for making lath support 42 is more steady, carrying handgrip 13 is provided with two, the locating dowel 131 of protrusion it is provided with inside carrying handgrip 13, locating dowel 131 can be cylindrical or square column type or other column constructions, in the present embodiment, locating dowel 131 is cylindrical, two locating dowels 131 it are provided with inside each carrying handgrip 13, it is formed with the guide edge 422 making way for locating dowel 131 along indent under lath support 42 two ends, guide edge 422 is provided with the locating slot 423 suitable with locating dowel 131, carrying handgrip 13 can slide along guide edge 422 under Sampling Machine mechanical arm 12 drives and snap in locating slot 423 by locating dowel 131 and moved on point model bar support 5 by the lath support 42 completing point sample on blank plates support 4.
Installing for convenience, a point sample assembly is provided with a plurality of guide rail 7 automatically, and consumptive material support 2, screed support 3, blank plates support 4 and a point model bar support 5 can be slidably mounted on corresponding guide rail 7 respectively, and installing/dismounting is the most more convenient.
Above-mentioned a kind of identify for mycoplasma and the workflow of point sample assembly automatically of Analysis of Drug Susceptibility, comprise the following steps:
A) Sampling Machine mechanical arm 12 moves at consumptive material support 2, and automatic pipettor 113 loads tip head, and liquid wax drawn by liquid feeding pin 112;
B) Sampling Machine mechanical arm 12 moves at 3 first samples of sample arm, and automatic pipettor 113 sucks the sample solution of set amount;
C) Sampling Machine mechanical arm 12 moves at 4 first laths 43 of blank plates support, uses bar code scan instrument 114 read the bar code value on lath 43 and sample information associated with this lath 43;Automatic pipettor 113 adds the sample solution of set amount successively in the first row of lath 43 detection hole 433 and second row detection hole 434, detection hole 432;
D) Sampling Machine mechanical arm 12 moves at 3 second samples of sample arm, and automatic pipettor 113 sucks the sample solution of set amount;
E) Sampling Machine mechanical arm 12 moves at 4 second laths 43 of blank plates support, uses bar code scan instrument 114 read the bar code value on lath 43 and sample information associated with this lath 43;Automatic pipettor 113 adds the sample solution of set amount successively in the first row of lath 43 detection hole 433 and second row detection hole 434, detection hole 432;Meanwhile liquid feeding pin 112 adds liquid wax by same order in first lath 43;
F) by step b~e, until having processed all of lath 43 on current lath support 42;
G) lath support 42 entirety having completed point sample on blank plates support 4 is carried at point model bar support 5 by carrying handgrip 13;
H) by step b~g, until having processed all of lath support 42 on blank plates support 4.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, as long as realizing within the technical scheme of the object of the invention broadly falls into protection scope of the present invention with essentially identical means.

Claims (8)

1. identify and point sample assembly automatically of Analysis of Drug Susceptibility for mycoplasma for one kind, it is characterized in that: include sample mobile platform, consumptive material support, sample arm, blank plates support, point model bar support and waste collection storehouse, described consumptive material support is placed with tip head and liquid wax, described sample arm is placed with the container equipped with sample solution, described sampling mobile platform can at consumptive material support, screed support, blank plates support, mobile point between model bar support and waste collection storehouse automatically divide sample.
The most according to claim 1 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: described sampling mobile platform includes sample charging mechanism, Sampling Machine mechanical arm and carrying handgrip, described Sampling Machine mechanical arm is driven by motor and is slidably mounted on the sampling slide rail that point sample assembly is provided with automatically, Sampling Machine mechanical arm is provided with the horizontal slide rail of mechanical arm and the vertical slide rail of mechanical arm, described sample charging mechanism and carrying handgrip and is driven by corresponding motor and be slidably mounted at respectively on the horizontal slide rail of mechanical arm and the vertical slide rail of mechanical arm.
The most according to claim 2 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: described sample charging mechanism is provided with sample-adding body frame, it is provided with liquid feeding pin on described sample-adding body frame, automatic pipettor and bar code scan instrument, described automatic pipettor can load tip head automatically at consumptive material support, tip head is unloaded in waste collection storehouse, sample solution is sucked in the container of sample arm, and the sample solution of suction is joined each Kong Weizhong of each lath on blank plates support, described liquid feeding pin is for carrying out sealing wax to the lath filling liquid wax being loaded, described bar code scan instrument for being scanned reading to lath information.
The most according to claim 3 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: be provided with two on described sample-adding body frame and be loaded vertical slide rail, described liquid feeding pin and bar code scan instrument driven by motor be slidably mounted at wherein one be loaded on vertical slide rail, described automatic pipettor is driven by motor and is slidably mounted at another and is loaded on vertical slide rail.
5. described a kind of identify and point sample assembly automatically of Analysis of Drug Susceptibility for mycoplasma according to claim 2 to 4 is arbitrary, it is characterized in that: described blank plates support is identical with the structure of point model bar support, all include lath stent bearing plank, multiple lath support it is stacked with on described lath stent bearing plank, uniform on described lath support arranging multiple lath mounting groove adaptive with lath, described lath is installed on lath mounting groove.
The most according to claim 5 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: described lath one end is provided with lath bar code district and detection hole, the lath other end is distributed up and down and is provided with first row detection hole and second row detection hole.
The most according to claim 5 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: described carrying handgrip is provided with two, the locating dowel of protrusion it is provided with inside carrying handgrip, it is formed with the guide edge making way for locating dowel along indent under described lath support two ends, described guide edge is provided with the locating slot suitable with locating dowel, and described carrying handgrip can slide along guide edge under machinery of sampling arm straps is dynamic and snap in locating slot by locating dowel and moved on point model bar support by the lath support completing point sample on blank plates support.
The most according to claim 1 a kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility, it is characterized in that: described automatic point of sample assembly is additionally provided with a plurality of guide rail, described consumptive material support, screed support, blank plates support and a point model bar support can be slidably mounted on corresponding guide rail respectively.
CN201610352595.6A 2016-05-24 2016-05-24 It is a kind of to divide sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility Active CN105838582B (en)

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CN106596992A (en) * 2017-02-15 2017-04-26 山东新华医疗器械股份有限公司 Multi-channel fully automatic blood type analyzer
CN107043696A (en) * 2017-05-05 2017-08-15 黄河科技学院 Automatically continuously culture detects screening plant to microorganism
CN107828648A (en) * 2017-11-07 2018-03-23 李昌伟 Continuously culture detects Analysis of Drug Susceptibility device to microorganism constant temperature automatically
CN110093250A (en) * 2019-05-14 2019-08-06 滕文友 A kind of full-automatic Culture Mycoplasma sample-adding detector and its culture sample-adding detection method

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CN105242055A (en) * 2014-05-21 2016-01-13 凯和生物科技(上海)有限公司 Improved strip-type immune fully-automatic analyzer
CN205774463U (en) * 2016-05-24 2016-12-07 江门市凯林贸易有限公司 A kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility

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CN103789198A (en) * 2014-02-27 2014-05-14 苏州天隆生物科技有限公司 Full automatic instrument for extracting nucleic acids
CN105242055A (en) * 2014-05-21 2016-01-13 凯和生物科技(上海)有限公司 Improved strip-type immune fully-automatic analyzer
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CN110093250A (en) * 2019-05-14 2019-08-06 滕文友 A kind of full-automatic Culture Mycoplasma sample-adding detector and its culture sample-adding detection method

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