CN105838582B - It is a kind of to divide sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility - Google Patents
It is a kind of to divide sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility Download PDFInfo
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- CN105838582B CN105838582B CN201610352595.6A CN201610352595A CN105838582B CN 105838582 B CN105838582 B CN 105838582B CN 201610352595 A CN201610352595 A CN 201610352595A CN 105838582 B CN105838582 B CN 105838582B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract
Dividing sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility the invention discloses a kind of, including sampling mobile platform, supplies support, sample arm, blank plates holder, divide sample lath support and waste collection storehouse, sampling mobile platform can be in supplies support, screed support, blank plates holder, mobile progress between sample lath support and waste collection storehouse is divided to divide sample automatically, manually sample is divided to operate instead of in traditional operation, substantially increase the efficiency of point sample, realize the automation mechanized operation for dividing sample in mycoplasma identification and Analysis of Drug Susceptibility, reduce manpower and operating time needed for mycoplasma identification and Analysis of Drug Susceptibility, a large amount of sample identification and Analysis of Drug Susceptibility can be realized with lower cost, meets the needs of hospital or related research institutes.
Description
Technical field
The present invention relates to mycoplasma identify field, more particularly, to it is a kind of for mycoplasma identification and Analysis of Drug Susceptibility it is automatic
Divide sample component.
Background technology
Mycoplasma is the minimum microorganism extracellularly to survive, is a kind of prokaryotic microorganism for lacking cell wall, greatly
It is small generally between 0.3~0.5um, be in height pleomorphism, have spherical shape, rod, filiform, a variety of states such as branched.It is different from
Cell, also different from virus, type is various, it is widely distributed, caused by endanger quite big, be related to people, animal, plant and insect etc.
Multiple fields bring adverse effect to human health and research work.The operation of mycoplasma identification at present and susceptibility detection, usually
It needs to be accomplished manually, main operating process is：Detected sample is added to nutrient broth by inoculation by swab of drawing materials
In；Mixing makes tested sample be mixed well with nutrient broth；Divide sample and seals paraffin, the battalion that sample will be inoculated with and mixed well
Foster meat soup is added in the hole of identification and medicine sensitive detecting plate, then seals up paraffin；It is incubated culture, by point good sample and seals paraffin
Detection plate be put into incubator be incubated certain time；Sentence read result changes judgement tested sample according to the color of each aperture
It is negative or positive, and to the sensitivity of various antibiotic.However manually-operated point of sample efficiency is relatively low, needs to compare
More operators operate, and especially in carrying out a large amount of sample identification and Analysis of Drug Susceptibility, the time and manpower needed is non-
Chang Duo so that mycoplasma is identified and the cost of Analysis of Drug Susceptibility is high, it is difficult to meet the needs of hospital.
Invention content
For overcome the deficiencies in the prior art, the present invention provides a kind of being used for mycoplasma identification and Analysis of Drug Susceptibility automatic point
Sample component has the function of dividing sample automatically, divides that sample is efficient, divides the sample used time short, at low cost.
The technical solution adopted in the present invention is mainly：It is a kind of to divide sample group for mycoplasma identification and the automatic of Analysis of Drug Susceptibility
Part, including sample mobile platform, supplies support, sample arm, blank plates holder, divide sample lath support and waste collection storehouse,
The supplies support is placed with tip and liquid wax, and the sample arm is placed with the container equipped with sample solution, the sampling
Mobile platform can supplies support, screed support, blank plates holder, divide between sample lath support and waste collection storehouse move into
Row divides sample automatically.
As an improvement of the above technical solution, the sampling mobile platform includes sample charging mechanism, machinery of sampling arm and removes
Transport handgrip, the machinery of sampling arm by motor drive be slidably mounted at automatically divide sample component to be equipped with sampling sliding rail on,
Machinery of sampling arm is provided with mechanical arm horizontal slide rail and mechanical arm vertical rails, and the sample charging mechanism and carrying handgrip pass through corresponding
Motor driving and be slidably mounted at respectively in mechanical arm horizontal slide rail and mechanical arm vertical rails.
As a further improvement of the above technical scheme, the sample charging mechanism is provided with sample-adding body frame, the sample-adding body frame
On be provided with liquid feeding pin, automatic pipettor and bar code scanner, the automatic pipettor can load tip automatically in supplies support
Head unloads tip in waste collection storehouse, sucks sample solution out of sample arm container, and by the sample solution of sucking
It is added to each Kong Weizhong of each lath on blank plates holder, the liquid feeding pin is used to fill liquid to the lath being loaded
Wax carries out sealing wax, and the bar code scanner to lath information for being scanned reading.
Further, two sample-adding vertical rails are provided on the sample-adding body frame, the liquid feeding pin and bar code scanner are logical
It crosses motor driving to be slidably mounted in wherein one sample-adding vertical rails, the automatic pipettor is driven slidably by motor
It is installed in another sample-adding vertical rails.
Further, the blank plates holder is identical with the structure of sample lath support is divided, and includes lath support supporting plate,
It is stacked with multiple lath supports on the lath support supporting plate, multiple plates being adapted to lath are uniformly arranged in the lath support
Mounting groove, the lath are installed on lath mounting groove.
Further, described lath one end is provided with lath bar code area and detection hole, and the lath other end is vertically disposed with first
It lists and indexes gaging hole and second row detection hole.
Further, there are two the carrying handgrip settings, the positioning column that protrusion is provided on the inside of handgrip, the lath are carried
Holder both ends lower edge is recessed the guide edge for making way for positioning column, and the guide edge is provided with compatible with positioning column fixed
Position slot, the carryings handgrip can under machinery of sampling armband is dynamic along guide edge sliding and by be caught in locating slot by positioning column incite somebody to action
It completes that the lath support of sample is divided to be moved in point sample lath support on blank plates holder.
Further, described to divide sample component to be additionally provided with a plurality of guide rail, the supplies support, screed support, blank plate automatically
Holder and sample lath support is divided to can be slidably mounted at respectively on corresponding guide rail.
The beneficial effects of the invention are as follows：
The a kind of of the present invention divides sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility, by being provided with sampling movement
Platform, sampling mobile platform can in supplies support, screed support, blank plates holder, divide sample lath support and waste collection storehouse
Between it is mobile carry out dividing sample automatically, divide sample to operate instead of artificial in traditional operation, substantially increase the efficiency of point sample, realization
The automation mechanized operation for dividing sample in mycoplasma identification and Analysis of Drug Susceptibility reduces people needed for mycoplasma identification and Analysis of Drug Susceptibility
Power and operating time can realize a large amount of sample identification and Analysis of Drug Susceptibility with lower cost, meet hospital or correlative study
The demand of mechanism.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the structural schematic diagram for sampling mobile platform；
The structural schematic diagram of Fig. 3 sample charging mechanisms；
Fig. 4 is a point structural schematic diagram for sample lath support；
Fig. 5 is lath support and the connection diagram of lath；
Fig. 6 is the structural schematic diagram of lath.
Specific implementation mode
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Referring to figs. 1 to Fig. 6, it is of the invention it is a kind of dividing sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility, including
Sampling mobile platform 1, sample arm 3, blank plates holder 4, divides sample lath support 5 and waste collection storehouse 6 at supplies support 2, consumes
Material holder 2 is placed with tip and liquid wax, and sample arm 3 is placed with the container equipped with sample solution, 4 He of blank plates holder
It is placed with lath 43 in point sample lath support 5, sampling mobile platform 1 tool, can be there are three the degree of freedom in vertical direction
Supplies support 2, blank plates holder 4, divides mobile between sample lath support 5 and waste collection storehouse 6 carry out automatically at screed support 3
Divide sample, to manually sample be divided to operate instead of in traditional operation, substantially increases the efficiency of point sample, realize mycoplasma identification
And divide the automation mechanized operation of sample in Analysis of Drug Susceptibility, when reducing manpower needed for mycoplasma identification and Analysis of Drug Susceptibility and operation
Between, a large amount of sample identification and Analysis of Drug Susceptibility can be realized with lower cost, meet the needs of hospital or related research institutes.
Specifically, sampling mobile platform 1 includes sample charging mechanism 11, machinery of sampling arm 12 and carries handgrip 13, it is automatic to divide
Sample component is provided with sampling sliding rail 14, and sampling sliding rail 14 can be provided with two, improve the stationarity of sliding, machinery of sampling arm 12
By motor drives be slidably mounted at sampling sliding rail 14 on, machinery of sampling arm 12 is provided with mechanical arm horizontal slide rail 15 and machine
Tool arm vertical rails 16, the sample charging mechanism 11 and carrying handgrip 13 are slidably mounted at respectively by corresponding motor drives
In mechanical arm horizontal slide rail 15 and mechanical arm vertical rails 16.In the present invention, above-mentioned motor is both provided with tape handler, adopts
Sample mechanical arm 12, sample charging mechanism 11 and carrying handgrip 13 are by the connection of the belt of tape handler slidably corresponding
Sliding rail on, certainly, above-mentioned component can also use chain drive or other kinds of drive, the present invention be not limited to this.
Sample charging mechanism 11 is provided with sample-adding body frame 111, is loaded on body frame 111 and is provided with liquid feeding pin 112, automatic pipettor
113 and bar code scanner 114, automatic pipettor 113 can load tip automatically in supplies support 2, be unloaded in waste collection storehouse 6
It carries tip, suck sample solution out of sample arm 3 container, and the sample solution of sucking is added to blank plates branch
Each Kong Weizhong of each lath 41 on frame 4, the liquid feeding pin 112 are used to fill liquid wax to the lath 41 being loaded and seal
Wax, bar code scanner 114 to 41 information of lath for being scanned reading, to close sample information and the lath 41
Connection.
To allow liquid feeding pin 112, automatic pipettor 113 and bar code scanner 114 in vertical convenient mobile, sample-adding
Two sample-adding vertical rails 115 are provided on body frame 111, the liquid feeding pin 112 and bar code scanner 114 can by motor driving
It is slidably mounted in wherein one sample-adding vertical rails 115, the automatic pipettor 113 is slidably mounted at by motor driving
In another sample-adding vertical rails 115.In the present invention, above-mentioned motor is both provided with tape handler, automatic pipettor 113,
Liquid feeding pin 112 and bar code scanner 114 be by with the connection of the belt of tape handler slidably on corresponding sliding rail,
Certainly, above-mentioned component can also use chain drive or other kinds of drive, the present invention to be not limited to this.
In the present invention, blank plates holder 4 is identical with the structure of sample lath support 5 is divided, and includes lath support supporting plate
41, multiple lath supports 42 are stacked on lath support supporting plate 41, are uniformly arranged and multiple are adapted to lath 43 in lath support 42
Lath mounting groove 421, lath 43 is installed on lath mounting groove 421, and 43 one end of lath is provided with lath bar code area 431 and inspection
Gaging hole 432,43 other end of lath are vertically disposed with first row detection hole 433 and second row detection hole 434.
To make the carrying of lath support 42 more steady, carries there are two the settings of handgrip 13, carry 13 inside of handgrip and set
It is equipped with the positioning column 131 of protrusion, positioning column 131 can be cylindrical or square column type or other column constructions, in the present embodiment
Positioning column 131 is cylindrical, positioning column 131 there are two settings on the inside of each carrying handgrip 13, in 42 both ends lower edge of lath support
Recessed to be formed with the guide edge 422 for making way for positioning column 131, guide edge 422 is provided with locating slot compatible with positioning column 131
423, carrying handgrip 13 can slide along guide edge 422 under the drive of machinery of sampling arm 12 and be caught in positioning by positioning column 131
Slot 423 and will on blank plates holder 4 complete divide the lath support 42 of sample to be moved in point sample lath support 5.
In order to facilitate the installation of, sample component is divided to be provided with a plurality of guide rail 7, supplies support 2, screed support 3, blank plates automatically
Holder 4 and sample lath support 5 is divided to can be slidably mounted at respectively on corresponding guide rail 7, installing and dismounting is all more convenient.
A kind of above-mentioned automatic workflow for dividing sample component being used for mycoplasma identification and Analysis of Drug Susceptibility, including following step
Suddenly：
A) machinery of sampling arm 12 moves at supplies support 2, and automatic pipettor 113 loads tip heads, and liquid feeding pin 112 is drawn
Liquid wax；
B) machinery of sampling arm 12 moves at 3 first samples of sample arm, and automatic pipettor 113 sucks the sample of set amount
This solution；
C) machinery of sampling arm 12 moves at 4 first laths 43 of blank plates holder, is read using bar code scanner 114
Sample information is simultaneously associated with by bar code value on lath 43 with the lath 43；Automatic pipettor 113 is successively in the first row of lath 43
The sample solution of set amount is added in detection hole 433 and second row detection hole 434, detection hole 432；
D) machinery of sampling arm 12 moves at 3 second samples of sample arm, and automatic pipettor 113 sucks the sample of set amount
This solution；
E) machinery of sampling arm 12 moves at 4 second laths 43 of blank plates holder, is read using bar code scanner 114
Sample information is simultaneously associated with by bar code value on lath 43 with the lath 43；Automatic pipettor 113 is successively in the first row of lath 43
The sample solution of set amount is added in detection hole 433 and second row detection hole 434, detection hole 432；At the same time liquid feeding pin 112
Liquid wax is added in first lath 43 by same sequence；
F) step b~e is pressed, until having handled lath 43 all in current lath support 42；
G) it carries handgrip 13 and 42 entirety of lath support that point sample is completed on blank plates holder 4 is carried to a point model item
At holder 5；
H) step b~g is pressed, until having handled lath support 42 all on blank plates holder 4.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, as long as with essentially identical
Means realize that the technical solution of the object of the invention belongs within protection scope of the present invention.
Claims (5)
1. a kind of dividing sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility, it is characterised in that：Including sampling mobile platform,
Supplies support, blank plates holder, divides sample lath support and waste collection storehouse, the supplies support to be placed with tip at sample arm
Head and liquid wax, the sample arm are placed with the container equipped with sample solution, and the sampling mobile platform can be in consumptive material branch
Frame, blank plates holder, divides mobile progress between sample lath support and waste collection storehouse to divide sample automatically at screed support, described to adopt
Sample mobile platform include sample charging mechanism, machinery of sampling arm and carry handgrip, the machinery of sampling arm by motor drives can
It is slidably mounted on the automatic sampling sliding rail for dividing sample component to be equipped with, machinery of sampling arm is provided with mechanical arm horizontal slide rail and mechanical arm
Vertical rails, the sample charging mechanism and carry handgrip by corresponding motor drives be slidably mounted at respectively mechanical arm transverse direction
On sliding rail and mechanical arm vertical rails；The blank plates holder is identical with the structure of sample lath support is divided, and includes lath
Stent bearing plank is stacked with multiple lath supports on the lath support supporting plate, multiple and plate is uniformly arranged in the lath support
The lath mounting groove of item adaptation, the lath are installed on lath mounting groove；There are two the carrying handgrip settings, carries handgrip
Inside is provided with the positioning column of protrusion, and lath support both ends lower edge is recessed the guide edge for making way for positioning column, institute
It states guide edge and is provided with locating slot compatible with positioning column, the carrying handgrip can be in the case where machinery of sampling armband be dynamic along leading
It is slided to side and will complete that the lath support of sample is divided to be moved to a point sample on blank plates holder by positioning column is caught in locating slot
In lath support.
A kind of sample component, feature is divided to exist for mycoplasma identification and the automatic of Analysis of Drug Susceptibility 2. according to claim 1
In：The sample charging mechanism is provided with sample-adding body frame, and liquid feeding pin, automatic pipettor and bar code scan are provided on the sample-adding body frame
Instrument, the automatic pipettor can load tip automatically in supplies support, unload tip, from sample arm in waste collection storehouse
Container in suck sample solution, and the sample solution of sucking is added to each hole of each lath on blank plates holder
In position, the liquid feeding pin is used to carry out sealing wax to the lath filling liquid wax being loaded, and the bar code scanner is used for lath
Information is scanned reading.
A kind of sample component, feature is divided to exist for mycoplasma identification and the automatic of Analysis of Drug Susceptibility 3. according to claim 2
In：Two sample-adding vertical rails are provided on the sample-adding body frame, the liquid feeding pin and bar code scanner can by motor driving
It is slidably mounted in wherein one sample-adding vertical rails, the automatic pipettor is slidably mounted at another by motor driving
It is loaded in vertical rails.
4. it is according to any one of claims 1 to 3 it is a kind of dividing sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility,
It is characterized in that：Described lath one end is provided with lath bar code area and detection hole, and the lath other end is distributed up and down is provided with first row
Detection hole and second row detection hole.
A kind of sample component, feature is divided to exist for mycoplasma identification and the automatic of Analysis of Drug Susceptibility 5. according to claim 1
In：It is described it is automatic divide sample component to be additionally provided with a plurality of guide rail, the supplies support, screed support, blank plates holder and divide sample
Lath support is can be slidably mounted at respectively on corresponding guide rail.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610352595.6A CN105838582B (en) | 2016-05-24 | 2016-05-24 | It is a kind of to divide sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility |
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| CN201610352595.6A CN105838582B (en) | 2016-05-24 | 2016-05-24 | It is a kind of to divide sample component for mycoplasma identification and the automatic of Analysis of Drug Susceptibility |
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| CN105838582B true CN105838582B (en) | 2018-10-23 |
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| CN106596992A (en) * | 2017-02-15 | 2017-04-26 | 山东新华医疗器械股份有限公司 | Multi-channel fully automatic blood type analyzer |
| CN107043696A (en) * | 2017-05-05 | 2017-08-15 | 黄河科技学院 | Automatically continuously culture detects screening plant to microorganism |
| CN107828648B (en) * | 2017-11-07 | 2021-02-26 | 河南方正医用电子有限公司 | Constant-temperature continuous automatic microbe culture and detection drug sensitivity analysis device |
| CN110093250A (en) * | 2019-05-14 | 2019-08-06 | 滕文友 | A kind of full-automatic Culture Mycoplasma sample-adding detector and its culture sample-adding detection method |
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| CN101203597A (en) * | 2005-04-21 | 2008-06-18 | 塞莱勒斯诊断公司 | Wicking cassette method and apparatus for automated rapid immunohistochemistry |
| CN103789198B (en) * | 2014-02-27 | 2015-09-16 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acid |
| CN105319378B (en) * | 2014-05-21 | 2017-02-22 | 凯和生物科技(上海)有限公司 | Batten type fully-automatic immunity analyzer |
| CN204874495U (en) * | 2015-07-31 | 2015-12-16 | 郑州点石生物技术有限公司 | Quick reagent board of appraisal medicine is cultivateed to mycoplasma |
| CN205774463U (en) * | 2016-05-24 | 2016-12-07 | 江门市凯林贸易有限公司 | A kind of identify for mycoplasma and the automatically point sample assembly of Analysis of Drug Susceptibility |
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