CN114371063B - Full-automatic magnetic solid phase extraction device - Google Patents
Full-automatic magnetic solid phase extraction device Download PDFInfo
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- CN114371063B CN114371063B CN202111589886.4A CN202111589886A CN114371063B CN 114371063 B CN114371063 B CN 114371063B CN 202111589886 A CN202111589886 A CN 202111589886A CN 114371063 B CN114371063 B CN 114371063B
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- 238000002414 normal-phase solid-phase extraction Methods 0.000 title claims abstract description 20
- 238000000605 extraction Methods 0.000 claims abstract description 159
- 239000002699 waste material Substances 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 22
- 238000001514 detection method Methods 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 230000005540 biological transmission Effects 0.000 claims description 65
- 230000001360 synchronised effect Effects 0.000 claims description 64
- 230000007246 mechanism Effects 0.000 claims description 59
- 239000011148 porous material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 description 10
- 230000033001 locomotion Effects 0.000 description 6
- 238000003825 pressing Methods 0.000 description 6
- 230000003014 reinforcing effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002787 reinforcement Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000037029 cross reaction Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 239000003351 stiffener Substances 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
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- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sampling And Sample Adjustment (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a full-automatic magnetic solid-phase extraction device which comprises a sample module, an information acquisition module, an X-direction driving assembly, a liquid transferring module, an extraction module, a waste module, a consumable module, a reagent module and a bottom plate, wherein the sample module is connected with the information acquisition module; the sample module, the information acquisition module, the X-direction driving assembly and the waste module are arranged on the bottom plate; the liquid transferring module and the extraction module are respectively connected with the X-direction driving component; the consumable module and the reagent module are fixed on the waste module; the pipetting module comprises a Y-direction driving assembly, a pipetting Z-direction driving assembly and a pipetting device; the pipetting Z-direction driving assembly is fixed on the Y-direction driving assembly; the pipettor is fixed on the pipetting Z-direction driving assembly; the extraction module is provided with a plurality of matched magnetic rod sleeves and magnetic rods. The automatic extraction treatment integrating information acquisition, pipetting, sample adding and extraction is realized, the labor intensity of manual intervention and operators is reduced, errors caused by manual operation are avoided, the repeatability is good, the uniformity is high, and the extraction efficiency and the accuracy of detection results are improved.
Description
Technical Field
The invention relates to the field of medical technical equipment, in particular to a full-automatic magnetic solid phase extraction device.
Background
The mass spectrum technology has the advantages of co-detection of various target analytes, strong anti-interference capability (anisotropic antibodies, autoantibodies, cross reactions), high specificity, high sensitivity and the like, so that the mass spectrum technology plays an increasing role in life histology, accurate medical treatment and clinical medicine, and the application of clinical medical examination is also increasing.
The mass spectrometry process is roughly divided into four steps of sample collection, sample pretreatment, detection analysis and data processing, and the sample pretreatment is the step with the longest occupied time and the easiest error introduction in the whole mass spectrometry process due to the fact that the sample extraction step is complex and time-consuming and the defect of poor uniformity in the sample pretreatment. Compared with extraction methods such as a liquid-liquid extraction method and a protein precipitation method, the method for transferring magnetic materials through magnetic solid phase extraction reduces the sample extraction time, and meanwhile, the method is more friendly to the requirements of a full-automatic sample processing platform. At present, the inspection mechanism uses a magnetic solid-phase extraction method to extract less samples, and the adding and transferring of the samples and the reagents are manually completed by professional operators, so that the inspection mechanism has the advantages of higher labor intensity, low efficiency, and poor uniformity caused by manual operation, and influences the accuracy of results.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a full-automatic magnetic solid phase extraction device, which realizes automatic extraction treatment integrating information acquisition, pipetting, sample adding and extraction.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
The invention provides a full-automatic magnetic solid-phase extraction device which comprises a sample module, an information acquisition module, an X-direction driving assembly, a liquid transferring module, an extraction module, a waste module, a consumable module, a reagent module and a bottom plate, wherein the sample module is connected with the information acquisition module; the sample module, the information acquisition module, the X-direction driving assembly and the waste module are arranged on a bottom plate; the liquid transferring module and the extraction module are respectively connected with the X-direction driving assembly; the consumable module and the reagent module are fixed on the waste module; wherein,,
the pipetting module comprises a Y-direction driving assembly, a pipetting Z-direction driving assembly and a pipetting device; the pipetting Z-direction driving assembly is fixed on the Y-direction driving assembly; the pipettor is fixed on the pipetting Z-direction driving assembly;
the extraction module is provided with a plurality of matched magnetic rod sleeves and magnetic rods, and the magnetic rod sleeves and the magnetic rods move along the Z direction respectively so as to extend into detection holes fixed on the consumable module to perform magnetic solid-phase extraction.
Preferably, the sample module comprises:
at least one sample rack slide rail mounted to the base plate;
at least one sample rack, which is provided with a plurality of mounting grooves for clamping the sample tubes;
the magnet mounting seat is fixed at one end of the sample rack sliding rail;
a first magnet mounted to an end of the sample holder facing the magnet mount;
a second magnet mounted to the magnet mount;
the indication assembly is used for emitting light or sound indication;
the sample frame sliding connection in the sample frame slide rail, when first magnet with the second magnet adsorbs so that sample frame end is fixed in the magnet mount pad, the instruction subassembly sends the instruction, in order to instruct the sample frame is installed in place.
Preferably, the X-direction driving assembly comprises a first driving mechanism and a second driving mechanism; wherein,,
the first driving mechanism comprises a first synchronous belt transmission mechanism and a first screw rod which are matched with each other; the first screw rod is matched with an extraction sliding block plate of the extraction module to form a transmission structure; the first synchronous belt transmission mechanism drives the first screw rod to rotate, so that the extraction sliding block plate drives the extraction module to move along the X direction;
The second driving mechanism comprises a second synchronous belt transmission mechanism and a second screw rod which are matched with each other; the second screw rod is matched with a pipetting slider plate of the pipetting module to form a transmission structure; the second synchronous belt transmission mechanism is used for driving the second screw rod to rotate, so that the pipetting slider plate drives the pipetting module to move along the X direction.
Preferably, the X-direction drive assembly further comprises at least two sets of guide members; the guide component comprises a linear guide rail, a first sliding block and a second sliding block; the linear guide rail extends along the X direction; the two first sliding blocks and the second sliding blocks are respectively and slidably connected with the linear guide rail and the extraction module and the pipetting module.
Preferably, the pipetting module comprises a pipetting Z-direction connecting plate and a pipetting slider plate and a pipetting Y-direction connecting plate which are connected with each other; the pipetting slide block plate is matched with the second screw rod and connected with the first slide block; the Y-direction driving assembly is arranged on the pipetting Y-direction connecting plate; the pipetting Z-direction driving assembly is arranged on the pipetting Z-direction connecting plate;
the Y-direction driving assembly comprises a third synchronous belt transmission mechanism and a pipetting Y-direction linear guide rail, and the pipetting Z-direction connecting plate is connected with the third synchronous belt transmission mechanism and is connected with the pipetting Y-direction linear guide rail in a sliding manner;
The pipetting Z-direction driving assembly comprises a fourth synchronous belt transmission mechanism, a pipetting Z-direction screw rod, a transmission sliding block and a pipetting Z-direction linear guide rail; the transmission sliding block is matched with the pipetting Z-direction screw rod to form a transmission structure; the transmission sliding block is connected with the pipetting Z-direction linear guide rail in a sliding manner and connected with the pipetting device.
Preferably, the extraction module comprises an extraction sliding block plate, an extraction connecting plate, an extraction Z-direction linear guide rail, a fifth sliding block, a sixth sliding block, a fifth synchronous belt transmission mechanism, a sixth synchronous belt transmission mechanism, a magnetic rod sleeve frame and a magnetic rod frame; wherein, a plurality of the magnetic rod sleeves are fixed on the magnetic rod sleeve frame, and a plurality of the magnetic rods are fixed on the magnetic rod frame;
the extraction sliding block plate is connected to the extraction connecting plate and is matched with the first screw rod to form a transmission structure;
the fifth sliding block and the sixth sliding block are respectively and slidably connected to the extraction Z-direction linear guide rail; the fifth sliding block and the sixth sliding block are respectively connected with the magnetic rod sleeve frame and the magnetic rod frame;
the fifth synchronous belt transmission mechanism is connected with the fifth sliding block so as to drive the fifth sliding block to drive the magnetic rod sleeve frame to move along the Y direction; the sixth synchronous belt transmission mechanism is respectively connected with the sixth sliding block so as to drive the sixth sliding block to drive the magnetic rod frame to move along the Y direction.
Preferably, the extraction module further comprises a baffle plate rotatably connected to the extraction slider plate or the extraction connection plate;
when extraction is carried out, the baffle rotates to be far away from the magnetic rod sleeve; after extraction is completed, the baffle rotates to be positioned below the magnetic rod sleeve so as to bear samples dripped on the surface of the magnetic rod sleeve.
Preferably, the magnetic rod is fixed on the magnetic rod frame through a buckle structure;
and/or the magnetic rod sleeve is fixed on the magnetic rod sleeve frame through a buckle structure.
Preferably, the waste module comprises a waste launch plate and a waste cartridge; the waste material is put in the board and is equipped with and leads to the opening of putting in of fertilizer box is used for putting in the abandonment tip rifle head of pipetting module.
Preferably, the waste module comprises a first consumable base and a second consumable base which are arranged in a staggered manner; the waste box is arranged below the first consumable base and the second consumable base; the waste throwing plate is positioned between the first consumable base and the second consumable base;
the consumable module comprises a pore plate kit and a tip fixing frame; the pore plate kit is clamped on the second consumable base; the tip fixing frame is fixed on the first consumable base through a fastener; the reagent module is fixed on the second consumable base.
Compared with the prior art, the application has the beneficial effects that:
the application provides a full-automatic magnetic solid-phase extraction device, which realizes automatic extraction treatment integrating information acquisition, pipetting, sample addition and extraction, reduces manual intervention and labor intensity of operators, avoids errors caused by manual operation, has good repeatability and high uniformity, and improves extraction efficiency and detection result accuracy.
The foregoing description is only an overview of the present application, and is intended to provide a better understanding of the technical means of the present application, and is to be implemented in accordance with the contents of the specification, as follows, in accordance with the preferred embodiments of the present application, as hereinafter described in detail with reference to the accompanying drawings. Specific embodiments of the present application are given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute a limitation on the application. In the drawings:
FIG. 1 is a schematic view of a part of a device body according to the present application;
FIG. 2 is a schematic perspective view of a sample module according to the present application;
FIG. 3 is a cross-sectional view of a sample module of the present application;
FIG. 4 is a schematic perspective view of an information acquisition module according to the present invention;
FIG. 5 is a schematic perspective view of an X-direction driving assembly according to the present invention;
FIG. 6 is a schematic perspective view of a pipetting module of the invention;
FIG. 7 is a cross-sectional view of a pipetting module of the invention;
FIG. 8 is a schematic perspective view of an extraction module according to the present invention;
FIG. 9 is a cross-sectional view of the extraction module of the present invention;
FIG. 10 is a schematic perspective view of a scrap module of the present invention;
FIG. 11 is a schematic diagram of the assembly structure of the consumable module, the reagent module and the consumable module according to the present invention;
fig. 12 is a schematic perspective view of a device body according to the present invention;
FIG. 13 is a schematic diagram showing the orientation of a magnetic rod sleeved in a 96-well plate kit when the extraction of the invention is four steps;
FIG. 14 is a schematic diagram showing the magnetic bar sleeved in a 96-well plate kit when the extraction of the invention is five steps;
FIG. 15 is a schematic diagram showing the orientation of a magnetic rod sleeved in a 96-well plate kit when the extraction of the invention is six steps.
In the figure: 100. a device body;
1. a housing;
2. a frame;
3. a sample module; 31. a sample rack slide rail; 32. a sample rack; 33. a circuit board mounting board; 34. a circuit board; 35. a magnet mounting base; 36. a first magnet; 37. a second magnet; 38. a slot-type optocoupler; 39. an indicator light;
4. An information acquisition module; 41. a code scanning gun; 42. a code scanning gun fixing plate;
5. an X-direction driving assembly; 51. a first motor; 52. a second motor; 53. a first X-direction connecting plate; 54. a second X-direction connecting plate; 55. a base; 56. a linear guide rail; 57. a first slider; 58. a second slider; 59. a first synchronization belt; 510. a second timing belt; 511. a first screw rod; 512. a second screw rod;
6. a pipetting module; 61. a Y-direction drive assembly; 611. a pipetting slider plate; 612. a pipetting Y-direction connecting plate; 613. pipetting Y-direction reinforcing ribs; 614. a third motor; 615. pipetting Y-direction linear guide rail; 616. a pipetting Y-direction slide block; 617. a liquid transferring Y-direction belt seat; 618. pipetting Y-direction synchronous belt; 619. a pipetting Y-direction belt pressing block; 62. a Z-direction drive assembly; 621. a pipetting Z-direction connecting plate; 622. a pipetting Z is downwards arranged on the mounting plate; 623. a pipetting Z-direction mounting plate; 624. a fourth motor; 625. pipetting Z-direction linear guide rail; 626. a third slider; 627. a fourth slider; 628. a third slider connecting seat; 629. a fourth slider connecting seat; 6210. a spring; 6211. a Z-direction screw rod for pipetting; 6212. an ADP mounting seat; 6213. a pipette;
7. an extraction module; 71. extracting a sliding block plate; 72. extracting a connecting plate; 73. a fifth motor; 74. a sixth motor; 75. extracting a Z-direction linear guide rail; 76. a fifth slider; 77. a sixth slider; 78. extracting the first synchronous belt; 79. extracting the second synchronous belt; 710. extracting the first belt seat; 711. extracting the second belt seat; 712. extracting a first belt press block; 713. extracting a second belt briquetting; 714. a magnetic rod sleeve frame; 715. reinforcing ribs of the magnetic rod sleeve frame; 716. a magnetic bar frame; 717. a magnetic bar frame reinforcing rib; 718. a magnetic rod; 719. a magnetic rod sleeve; 720. a seventh motor mounting plate; 721. a seventh motor; 722. a baffle connecting block; 723. a baffle;
8. A waste module; 81. a consumable holder; 82. a first consumable base; 83. a second consumable base; 84. a waste material throwing plate; 85. a waste bin;
9. a consumable module; 91. a 96-well plate kit; 92. a tip upper support frame; 93. a tip lower support frame; 94. tip gun head frame;
10. a reagent module;
11. a bottom plate.
Detailed Description
The foregoing and other objects, features, aspects and advantages of the present invention will become more apparent to those skilled in the art from the following detailed description, which, taken in conjunction with the annexed drawings, discloses a device for practicing the invention. In the drawings, the shape and size may be exaggerated for clarity, and the same reference numerals will be used throughout the drawings to designate the same or similar components. In the following description, terms such as center, thickness, height, length, front, back, rear, left, right, top, bottom, upper, lower, etc. are based on the orientation or positional relationship shown in the drawings. In particular, "height" corresponds to the top-to-bottom dimension, "width" corresponds to the left-to-right dimension, and "depth" corresponds to the front-to-back dimension. These relative terms are for convenience of description and are not generally intended to require a particular orientation. Terms (e.g., "connected" and "attached") referring to an attachment, coupling, etc., refer to a relationship wherein these structures are directly or indirectly secured or attached to one another through intervening structures, as well as both movable or rigid attachments or relationships, unless expressly described otherwise.
The present invention will be further described with reference to the accompanying drawings and detailed description, wherein it is to be understood that, on the premise of no conflict, the following embodiments or technical features may be arbitrarily combined to form new embodiments.
Example 1
The invention provides a full-automatic magnetic solid-phase extraction device, as shown in figures 1, 4, 5 and 11, which comprises a device body 100, wherein the device body 100 comprises a sample module 3, an information acquisition module 4, an X-direction driving component 5, a pipetting module 6, an extraction module 7, a waste module 8, a consumable module 9, a reagent module 10 and a bottom plate 11; the sample module 3, the information acquisition module 4, the X-direction driving component 5 and the waste module 8 are arranged on a bottom plate 11; the pipetting module 6 and the extraction module 7 are respectively connected with the X-direction driving component 5; the consumable module 9 and the reagent module 10 are fixed on the waste module 8; wherein,,
the pipetting module 6 comprises a Y-direction driving assembly 61, a pipetting Z-direction driving assembly 62 and a pipetting device 6213; the pipetting Z-direction driving assembly 62 is fixed on the Y-direction driving assembly 61; the pipette 6213 is affixed to the pipetting Z-drive assembly 62;
the extraction module 7 is provided with a plurality of matched magnetic rod sleeves 719 and magnetic rods 718, and the magnetic rod sleeves 719 and the magnetic rods 718 respectively move along the Z direction so as to extend into detection holes fixed on the consumable module 9 to perform magnetic solid-phase extraction.
In this embodiment, the device body 100 realizes the automatic extraction processing of integrating information collection, pipetting and extraction, reduces the labor intensity of manual intervention and operators, avoids errors caused by manual operation, has good repeatability and high uniformity, and improves the extraction efficiency and the accuracy of detection results.
In an embodiment, the device body 100 further includes a frame 2 for forming a supporting framework of the device body 100.
In an embodiment, as shown in fig. 12, the device body 100 further includes a housing 1 for forming an outer contour structure of the device body 100, thereby improving the aesthetic appearance and dust and water resistance.
In one embodiment, the top wall of the interior of the housing 1 is provided with an ultraviolet sterilizing lamp, and a sterilizing mode is started after the daily extraction work is completed to sterilize, so as to prevent cross contamination of the samples. Further, the ultraviolet sterilizing lamp is an ozone-free sterilizing lamp.
In one embodiment, as shown in fig. 2 and 3, the sample module 3 includes:
at least one sample rack slide rail 31 mounted to the base plate 11;
at least one sample rack 32, wherein the sample rack 32 is provided with a plurality of mounting grooves for clamping sample tubes;
a magnet mount 35 fixed to one end of the sample rack slide rail 31;
A first magnet 36 mounted to an end of the sample holder 32 facing the magnet mount 35;
a second magnet 37 mounted on the magnet mount 35;
the indication assembly is used for emitting light or sound indication;
the sample rack 32 is slidably connected to the sample rack slide rail 31, and when the first magnet 36 and the second magnet 37 are attracted to make the end of the sample rack 32 fixed to the magnet mounting seat 35, the indication component sends an indication to indicate that the sample rack 32 is mounted in place. Specifically, the sample rack 32 is fast assembled and disassembled by clamping the sample rack slide rail 31 with the sample rack 32, and the operation is simple and convenient. The first magnet 36 and the second magnet 37 improve the assembling stability of the sample rack sliding rail 31 and the sample rack 32, and are convenient to detach. An indicator assembly is provided to indicate whether the sample holder 32 is in place or not to prevent the sample holder 32 from being out of place and affecting subsequent sampling.
In a specific embodiment, the sample module 3 includes a sample rack slide rail 31, a sample rack 32, a circuit board mounting plate 33, a circuit board 34, a magnet mounting seat 35, a first magnet 36, a second magnet 37, a slot optocoupler 38, and an indicator light 39; sample frame slide rail 31 passes through the screw fixation in bottom plate 11, sample frame 32 pass through its bottom draw-in groove install in sample frame slide rail 31, circuit board mounting panel 33 passes through the screw fixation in bottom plate 11, circuit board 34 passes through the screw fixation in circuit board mounting panel 33, magnet mount pad 35 passes through the screw fixation in circuit board mounting panel 33, first magnet 36 install in sample frame 32, second magnet 37 passes through the screw fixation in magnet mount pad 35, slot type opto-coupler 38 with pilot lamp 39 passes through the welding to be fixed in circuit board 34. The circuit board mounting plate 33, the circuit board 34, the slot optical coupler 38, and the indicator lamp 39 constitute the indicator assembly.
Further, the sample rack slide rail 31, the sample rack 32, the slot-type optocoupler 38 and the indicator lamp 39 are respectively mounted in three and have a one-to-one correspondence, when one sample rack 32 is mounted on one sample rack slide rail 31 through a clamping slot, one slot-type optocoupler 38 is triggered, so that one indicator lamp 39 is triggered, which indicates that the sample rack 32 is placed at the position, and is fixed through the mutual attraction of the first magnet 36 mounted on the sample rack 32 and the second magnet 37 mounted on the magnet mounting seat 35.
Further, the sample rack 32 is provided with eight mounting grooves, the eight mounting grooves are uniformly arranged in a row, and the three sample racks 32 are arranged in parallel to form a 3×8 mounting groove arrangement structure, so that 24 samples can be processed at most at one time. Further, the sample holders 32 may also be four, five or even more to increase the number of samples that can be processed at one time.
In one embodiment, as shown in fig. 4, the information collecting module 4 includes a barcode scanning gun 41 and a barcode scanning gun fixing plate 42, the barcode scanning gun fixing plate 42 is fixed on the bottom plate 11 by a screw, the barcode scanning gun 41 is fixed on the barcode scanning gun fixing plate 42 by a screw, when a sample tube with a barcode is placed on the sample frame 32 and put in, the barcode scanning gun 41 recognizes barcode information on the sample tube, wherein a blank barcode is arranged between adjacent holes of the sample frame 32 for distinguishing the barcodes of the adjacent sample tubes.
In one embodiment, as shown in fig. 5, the X-direction driving assembly 5 includes a first driving mechanism and a second driving mechanism; wherein,,
the first driving mechanism comprises a first synchronous belt transmission mechanism and a first screw rod 511 which are matched with each other; the first screw rod 511 is matched with the extraction sliding block plate 71 of the extraction module 7 to form a transmission structure; the first synchronous belt transmission mechanism drives the first screw rod 511 to rotate, so that the extraction sliding block plate 71 drives the extraction module 7 to move along the X direction;
the second driving mechanism comprises a second synchronous belt transmission mechanism and a second screw rod 512 which are matched; the second screw rod 512 is matched with the pipetting slider plate 611 of the pipetting module 6 to form a transmission structure; the second synchronous belt transmission mechanism is used for driving the second screw rod 511 to rotate, so that the pipetting slider plate 611 drives the pipetting module 6 to move along the X direction. Specifically, the X-direction driving assembly 5 is driven by two groups of driving mechanisms to respectively drive the extraction module 7 and the pipetting module 6 to move in the X-direction, so that the integration level is high, and the miniaturization design of the X-direction driving assembly 5 is facilitated. In addition, the synchronous belt transmission mechanism provides a rotary driving force, and is simple in structure, easy to assemble and stable in transmission. The first screw rod 511 is matched with the extraction sliding block plate 71, so that the linear motion of the extraction sliding block plate 71 is driven; the second screw rod 512 is matched with the pipetting slider plate 611 to realize the driving of the linear motion of the pipetting slider plate 611; simple structure, drive are stable to make extraction module 7 or pipetting module 6 can firmly follow X direction motion.
Further, the X-direction driving assembly further comprises at least two groups of guiding components; the guide part comprises a linear guide rail, a first sliding block 57 and a second sliding block 58; the linear guide rail extends along the X direction; the two first sliding blocks and the second sliding blocks are respectively and slidably connected with the linear guide rail and the extraction module 7 and the pipetting module 6. Specifically, the guiding component provides a supporting force for the extraction module 7 and the pipetting module 6 during movement, so that the service life of the X-direction driving assembly is prolonged. In addition, the guide parts are at least two groups to further improve the supporting performance.
In a specific embodiment, the X-direction driving assembly 5 includes a first motor 51, a second motor 52, a first X-direction connecting plate 53, a second X-direction connecting plate 54, two bases 55, two linear guides 56, two first sliders 57, two second sliders 58, a first synchronous belt 59, a second synchronous belt 510, a first screw rod 511, and a second screw rod 512, the bases 55 are fixed to the base plate 11 by screws, the linear guides 56 are respectively fixed to the bases 55 by screws, the first sliders 57 and the second sliders 58 are respectively fixed to the linear guides 56, the first X-direction connecting plate 53 and the second X-direction connecting plate 54 are respectively fixed to two ends of the bases 55 by screws, the first motor 51 is fixed to the first X-direction connecting plate 53 by screws, the second motor 52 is fixed to the second X-direction connecting plate 54 by screws, and the first screw rod 511 and the second screw rod 512 are respectively mounted between the first X-direction connecting plate 53 and the second X-direction connecting plate 54. The first synchronous belt transmission mechanism comprises a first motor 51, a first synchronous belt 59, a first driving wheel and a first driven wheel, wherein the first driving wheel is coaxially connected with an output shaft of the first motor 51; the first driven wheel is coaxially connected with a first screw rod 511; one end of the first synchronous belt 59 is sleeved on the first driving wheel, and the other end is sleeved on the first driven wheel. The second synchronous belt transmission mechanism comprises a second motor 52, a second synchronous belt 510, a second driving wheel and a second driven wheel, wherein the second driving wheel is coaxially connected with an output shaft of the second motor 52; the second driven wheel is coaxially connected with a second screw rod 512; one end of the second synchronous belt 510 is sleeved on the second driving wheel, and the other end is sleeved on the second screw rod 512. The first motor 51 drives the first screw rod 511 to rotate by driving the first synchronous belt 59; the second motor 52 drives the second screw rod 512 to rotate by driving the second synchronous belt 510.
In one embodiment, as shown in fig. 6 and 7, the pipetting module 6 includes a pipetting Z-direction connection plate 621, a connected pipetting slide plate 611 and a pipetting Y-direction connection plate 612; the pipetting slider plate 611 is matched with the second screw 511 and connected to the first slider; wherein the Y-direction driving component 61 is arranged on the pipetting Y-direction connecting plate 612; the pipetting Z-direction driving component 62 is arranged on the pipetting Z-direction connecting plate 621;
the Y-direction driving assembly 61 comprises a third synchronous belt transmission mechanism and a pipetting Y-direction linear guide rail 615, and the pipetting Z-direction connecting plate 621 is connected with the third synchronous belt transmission mechanism and is connected with the pipetting Y-direction linear guide rail 615 in a sliding manner;
the pipetting Z-direction driving assembly 62 comprises a fourth synchronous belt transmission mechanism, a pipetting Z-direction screw rod, a transmission sliding block and a pipetting Z-direction linear guide rail 625; the transmission sliding block is matched with the pipetting Z-direction screw rod to form a transmission structure; the drive slide is slidably coupled to the pipetting Z-directed linear guide 625 and to the pipettor 6213. Specifically, the third synchronous belt transmission mechanism drives the pipetting Z-direction connecting plate 621 to drive the pipetting device 6213 to move along the Y-direction, and the pipetting Y-direction linear guide rail 615 provides the Y-direction movement supporting force, so that the pipetting device is simple in structure and stable in operation. The fourth synchronous belt transmission mechanism drives the pipette 6213 to move along the Z direction, and the movement supporting force of Z is provided by the pipetting Z direction linear guide rail 625, so that the structure is simple and the operation is stable.
In a specific embodiment, the pipetting module 6 includes a pipetting slide plate 611, a pipetting Y-direction connection plate 612, a pipetting Y-direction stiffener 613, a third motor 614, a pipetting Y-direction linear guide 615, a pipetting Y-direction slide 616, a pipetting Y-direction belt seat 617, a pipetting Y-direction timing belt 618, a pipetting Y-direction belt press 619, a pipetting Z-direction connection plate 621, a pipetting Z-direction mounting plate 622, a pipetting Z-direction mounting plate 623, a fourth motor 624, a pipetting Z-direction linear guide 625, a third slide 626, a fourth slide 627, a third slide connection seat 628, a fourth slide connection seat 629, a spring 6210, a pipetting Z-direction screw 6211, an ADP mounting seat 6212, and a pipettor 6213; wherein,,
the pipetting slider plate 611 is fixed to the second slider 58 by a screw and is fixed to the second screw rod 512, and the second motor 52 drives the second screw rod 512 to rotate by driving the second synchronous belt 510, so that the pipetting slider plate 611 drives the pipetting module 6 to move along the linear guide rail 56 in the X-direction; the pipetting Y-direction connecting plate 612 is fixed on the pipetting slider plate 611 through screws and is fixedly connected through the pipetting Y-direction reinforcing ribs 613; the third motor 614 is fixed to the pipetting Y-direction connecting plate 612 by screws; the pipetting Y-direction linear guide 615 is fixed to the pipetting Y-direction connecting plate 612 by a screw, the pipetting Y-direction slider 616 is mounted on the pipetting Y-direction linear guide 615, and the pipetting Y-direction belt seat 617 is fixed to the pipetting Y-direction slider 616 by a screw; the pipetting Y-direction synchronous belt 618 is fixed on the pipetting Y-direction belt seat 617 through the pipetting Y-direction belt pressing block 619; the pipetting Z-direction connecting plate 621 is fixed on a pipetting Y-direction belt pressing block 619 through a screw, and the third motor 614 drives the pipetting Y-direction belt pressing block 619 to move along the pipetting Y-direction linear guide rail 615 in the Y-direction by driving the pipetting Y-direction synchronous belt 618; the pipetting Z upward mounting plate 623 and the pipetting Z downward mounting plate 622 are respectively fixed at two ends of the pipetting Z connecting plate 621 by screws; the fourth motor 624 is fixed to the pipetting Z-direction mounting plate 623 by screws; the pipetting Z-direction linear guide 625 is fixed to the pipetting Z-direction connecting plate 621 by a screw, and the third slider 626 and the fourth slider 627 are fixed to the pipetting Z-direction linear guide 625 by a screw; the third slider connecting seat 628 is fixed to the third slider 626 by a screw, and the fourth slider connecting seat 629 is fixed to the fourth slider 627 by a screw; the third slide block connecting seat 628 is fixed on the pipetting Z-direction screw rod 6211, the third slide block connecting seat 628 is connected with the fourth slide block connecting seat 629 through the spring 6210, the ADP mounting seat 6212 is fixed on the fourth slide block connecting seat 629 through a screw, and the pipettor 6213 is fixed on the ADP mounting seat 6212 through a screw; the fourth motor 624 drives the pipetting Z-direction screw rod 6211 to drive the ADP mounting seat 6212, so that the pipettor 6213 fixed on the ADP mounting seat 6212 moves along the pipetting Z-direction linear guide rail in the Z-direction, and meanwhile, the pipetting module 6 can move along the X-direction and the Z-direction driving component 62 can move along the Y-direction, so that the pipettor 6213 can move in three directions of XYZ to complete the functions of sample transfer and reagent addition. The third synchronous belt transmission mechanism comprises a third motor 614, a third driving wheel, a pipetting Y-direction synchronous belt 618, a third driven wheel and a pipetting Y-direction belt pressing block 619; the pipetting Y-direction belt press 619 is fixed to the pipetting Y-direction belt seat 617, and the pipetting Y-direction belt seat 617 is fixed to the pipetting Y-direction slider 616. The fourth synchronous belt transmission mechanism comprises a fourth motor 624, a fourth driving wheel, a pipetting Y-direction synchronous belt 618 and a fourth driven wheel; the fourth driven wheel is coaxially connected with the pipetting Z-direction screw 6211.
In one embodiment, as shown in fig. 8 and 9, the extraction module 7 includes an extraction slider plate 71, an extraction connection plate 72, an extraction Z-direction linear guide 75, a fifth slider 76, a sixth slider 77, a fifth synchronous belt transmission mechanism, a sixth synchronous belt transmission mechanism, a magnetic rod rack 714, and a magnetic rod rack 716; wherein a plurality of the magnetic rod sleeves 719 are fixed to the magnetic rod sleeve frame 714, and a plurality of the magnetic rods 718 are fixed to the magnetic rod frame 716;
the extraction slider plate 71 is connected to the extraction connection plate 72 and is matched with the first screw rod 511 to form a transmission structure;
the fifth slider 76 and the sixth slider 77 are respectively slidably connected to the extraction Z-direction linear guide 75; the fifth slider 76 and the sixth slider 77 are respectively connected to the magnetic rod rack 714 and the magnetic rod rack 716;
the fifth synchronous belt transmission mechanism is connected to the fifth slider 76 to drive the magnetic rod rack 714 to move along the Y direction; the sixth synchronous belt transmission mechanisms are respectively connected to the sixth sliding blocks 77, so as to drive the sixth sliding blocks 77 to drive the magnetic rod frame 716 to move along the Y direction. Specifically, the magnetic solid phase extraction is realized through the matching of the magnetic rod sleeve 719 and the magnetic rod 718, and the operation is simple, convenient and quick. The magnetic rod sleeve 719 and the magnetic rod 718 respectively move in the Z direction through the fifth synchronous belt transmission mechanism and the sixth synchronous belt transmission mechanism, so that the magnetic rod sleeve 719 and the magnetic rod 718 are close to or far away from each other, and extraction operation is further realized. The magnetic rod sleeve 719 and the magnetic rod 718 are respectively fixed through the magnetic rod sleeve 714 and the magnetic rod 716, and the magnetic rod sleeve 714 and the magnetic rod 716 are simple in structure and small in occupied space. The fifth sliding block 76 and the sixth sliding block 77 are respectively in sliding fit with the extraction Z-direction linear guide rail 75, so that the running stability of the magnetic rod sleeve frame 714 and the magnetic rod frame 716 is improved.
Further, the extraction module 7 further comprises a baffle 723 rotatably connected to the extraction slider plate 71 or the extraction connection plate 72;
during extraction, the baffle 723 rotates away from the bar sleeve 719; after extraction is completed, the baffle 723 rotates to be located below the bar magnet sleeve 719 to carry the sample dropped on the surface of the bar magnet sleeve 719. Specifically, the baffle 723 is provided to prevent the sample on the surface of the magnetic rod 719 from dripping into the rest of the sample after the completion of the extraction of a certain sample, effectively preventing cross contamination. In addition, the baffle 723 is simple and compact in structure, which is advantageous for the miniaturized design of the extraction module 7.
In one embodiment, the magnetic rod 718 is secured to the magnetic rod frame 716 by a snap-fit structure;
and/or the bar magnet sleeve 719 is secured to the bar magnet sleeve frame 714 via a snap-fit structure. Specifically, the snap-fit attachment structure facilitates assembly and disassembly, and facilitates cleaning or replacement of the magnetic rod 718 and/or the magnetic rod sleeve 719.
In a specific embodiment, the extraction module 7 includes an extraction slider plate 71, an extraction connection plate 72, a fifth motor 73, a sixth motor 74, an extraction Z-direction linear guide 75, a fifth slider 76, a sixth slider 77, an extraction first timing belt 78, an extraction second timing belt 79, an extraction first belt seat 710, an extraction second belt seat 711, an extraction first belt press 712, an extraction second belt press 713, a magnetic rod sleeve frame 714, a magnetic rod sleeve frame reinforcement 715, a magnetic rod frame 716, a magnetic rod frame reinforcement 717, a magnetic rod 718, a magnetic rod sleeve 719, a seventh motor mounting plate 720, a seventh motor 721, a baffle connection block 722, and a baffle 723; wherein,,
The extraction slider plate 71 is fixed to the first slider 57 by a screw, and is fixed to the first screw rod 511, and the first motor 51 drives the first screw rod 511 to rotate by driving the first synchronous belt 59, so that the extraction slider plate 71 drives the extraction module 7 to move along the linear guide rail 56 in the X-direction; the extraction connecting plate 72 is fixed to the extraction slider plate 71 by screws; the fifth motor 73 and the sixth motor 74 are fixed to the extraction connection plate 72 by screws; the extraction Z-direction linear guide rail 75 is fixed on the extraction connecting plate 72 through screws; the fifth slider 76 and the sixth slider 77 are respectively fixed to the extraction Z-direction linear guide 75 by screws; the first extraction belt seat 710 is fixed to the fifth slider 76 by a screw, and the first extraction timing belt 78 is fixed to the first extraction belt seat 710 by the first extraction belt press 712; the extraction second belt seat 711 is fixed to the sixth slider 77 by a screw, and the extraction second timing belt 79 is fixed to the extraction second belt seat 711 by the extraction second belt press 713; the magnetic rod rack 714 is fixed to the extraction first belt seat 710 by screws and is fixedly connected by the magnetic rod rack reinforcing ribs 715; the magnetic bar frame 716 is fixed on the extraction second belt seat 711 by screws and is fixedly connected by the magnetic bar frame reinforcing ribs 717; the bar magnet sleeve 719 is fixed to the bar magnet sleeve frame 714 by a snap, and the bar magnet 718 is fixed to the bar magnet frame 716 by a snap. The fifth synchronous belt transmission mechanism comprises a fifth motor 73, a fifth driving wheel, an extraction first synchronous belt 78, a fifth driven wheel and an extraction first belt pressing block 712; extraction first belt press 712 is secured to extraction first timing belt 78. The sixth synchronous belt transmission mechanism comprises a sixth motor 74, a sixth driving wheel, an extraction second synchronous belt 79, a sixth driven wheel and an extraction second belt press block 713; the extraction second timing belt 79 is fixed to the extraction second belt press 713. In the extraction operation process, the fifth motor 73 drives the first extraction timing belt 78 to drive the first extraction belt seat 710, so that the magnetic rod sleeve 719 mounted on the magnetic rod sleeve rack 714 moves along the Z direction, and the mixing and transferring functions in the extraction process are completed. The sixth motor 74 drives the extraction second synchronous belt 79 to drive the extraction second belt seat 711, so that the magnetic rod 718 mounted on the magnetic rod frame 716 moves along the Z direction to complete the adsorption and transfer functions in the extraction process, and the extraction module 7 moves along the X direction, so that the extraction module 7 completes the extraction function.
The seventh motor mounting plate 720 is fixed to the extraction slider plate 71 through screws, the seventh motor 721 is fixed to the seventh motor mounting plate 720 through screws, the baffle connecting block 722 is fixed to a motor shaft of the seventh motor 721 through screws, the baffle 723 is fixed to the baffle connecting block 722 through screws, during an extraction process, the seventh motor 721 drives the baffle 723 to rotate and move away, so that the magnetic rod 718 and the magnetic rod sleeve 719 complete an extraction function, after the extraction process is completed, the seventh motor 721 drives the baffle 723 to rotate to an initial position, so that cross contamination caused by dropping of samples adhered to the magnetic rod sleeve 719 in other samples is avoided, and finally the extraction module 7 moves to the initial position along the X direction.
In one embodiment, as shown in fig. 10, the waste module 8 includes a waste feed plate 84, a waste cartridge 85; the waste material throwing plate 84 is provided with a throwing opening leading to the fertilizer box 85, and is used for throwing in the waste tip gun heads of the pipetting module 6 so as to automatically collect the waste tip gun heads, reduce manual operation and improve extraction efficiency.
In an embodiment, as shown in fig. 10 and 11, the waste module 8 includes a first consumable substrate seat 82 and a second consumable substrate seat 83 that are disposed in a staggered manner; the waste box 85 is arranged below the first consumable base 82 and the second consumable base 83; the waste feeding plate 84 is located between the first consumable part base 82 and the second consumable part base 83;
The consumable module 9 comprises a 96-well plate kit 91 and a tip fixing frame; the 96-well plate kit 91 is clamped on the second consumable base 83; the tip fixing frame is fixed on the first consumable part base 82 through a fastener; the reagent module 10 is fixed to a second consumable base 83. Specifically, the tip gun head is generally longer, the first consumable base 82 and the second consumable base 83 are arranged at a higher level, and the first consumable base 82 is higher than the second consumable base 83, so that the height difference between the tip fixing frame and the 96-well plate kit 91 and the reagent module 10 is reduced, the distance of the pipetting module 6 in the Z direction is reduced in the process of mounting the tip gun head, transferring samples and discarding the discarded tip gun head, and the operation efficiency is further improved. During detection, the magnetic rod sleeve 719 is used for extending into a hole of the 96-well plate kit 91 to perform magnetic attraction, and after the step is finished, the magnetic rod 718 extends into the magnetic rod sleeve 719 to adsorb the magnetic rod sleeve 719 out of the corresponding hole. The 96-well plate kit 91 is matched with the extraction module 7, and can operate the extraction of a plurality of samples at one time, so that the extraction efficiency is high.
In one embodiment, as shown in fig. 11, the reagent module 10 has a reagent tank structure, and the number of reagent tanks is at least two, so as to respectively mount a reagent tube containing an internal standard solution and a reagent tube containing a diluent solution.
In a specific embodiment, as shown in fig. 10, the waste module 8 includes two consumable holders 81, a first consumable holder 82, a second consumable holder 83, a waste inlet plate waste feeding plate 84, and a waste box 85, the consumable holders 81 are fixed on the bottom plate 11 by screws, the first consumable holders 82 are respectively fixed on the two consumable holders 81 by screws, the second consumable holder 83 is fixed on the two consumable holders 81 by screws, the waste inlet plate waste feeding plate 84 is fixed on the second consumable holder 83 by screws, the waste box 85 is placed between the two consumable holders 81, the bottom plate 11, the first consumable holder 82, and the second consumable holder 83, in the sample transferring and reagent adding process, the pipettor 6213 is completed by using disposable consumables (tip gun heads), and through inlet holes on the waste inlet plate waste feeding plate 84, the discarding action is completed, and finally the waste box is taken out by an operator to discard the tip gun heads.
In one embodiment, as shown in fig. 10 and 11, the tip holder includes a tip upper support 92, a tip lower support 93, and a tip gun head 94; the tip gun head frame 94 is provided with a plurality of loading holes for being arranged on the tip gun head; the 96-well plate kit 91 is placed in the clamping groove of the first consumable base 82, the tip lower support frame 93 is fixed to the second consumable base 83 through screws, the tip upper support frame 92 is fixed to the tip lower support frame 93 through screws, the tip gun head frame 94 is placed in the tip upper support frame 92, and the 96-well plate kit 91 and the tip gun head frame 94 are placed in manually by operators.
When the extraction step comprises four steps of sample adding, first leaching, second leaching and eluting, the magnetic rod frame 716 is loaded with three columns of magnetic rods 718, eight magnetic rods 718 in each column, and twenty-four magnetic rods 718 in total; the number and positions of the bar magnet sleeve 719 correspond to the number and positions of the bar magnet 718. As shown in fig. 13, in the extraction, three columns of bar magnet sleeves 719 correspond to the first column of holes, the fifth column of holes, and the ninth column of holes of the 96-well plate kit 91, respectively, so as to extract twenty-four samples in common. During detection, the first row of magnetic rod sleeves 719 sequentially pass through the first row of holes, the second row of holes, the third row of holes and the fourth row of holes to sequentially complete the four-step operation. Similarly, the second row of bar magnet sleeves 719 and the third row of bar magnet sleeves 719 pass through the four rows of holes in sequence respectively, so that the four steps of operation are completed in sequence. The extraction of twenty-four samples can be operated at one time, and the extraction efficiency is high.
When the extraction step includes five steps (e.g., one additional elution), the magnetic rod rack 716 is loaded with two columns of eight magnetic rods 718, for a total of sixteen magnetic rods 718; the number and positions of the bar magnet sleeve 719 correspond to the number and positions of the bar magnet 718. As shown in fig. 14, during extraction, two rows of magnetic bar sleeves 719 correspond to the first row of holes and the seventh row of holes of the 96-well plate kit 91, respectively, so as to extract sixteen samples together. During detection, the first row of magnetic rod sleeves 719 sequentially pass through the first row of holes, the second row of holes, the third row of holes, the fourth row of holes and the fifth row of holes to sequentially complete the operation of five steps. The second row of bar magnet sleeves 719 sequentially pass through the five rows of holes from the seventh row of holes, completing the five steps in sequence. Sixteen samples can be extracted at one time, and the extraction efficiency is high.
When the extraction step includes six steps, the magnetic bar frame 716 loads two columns of magnetic bars 718, eight magnetic bars 718 per column, sixteen magnetic bars 718 in total; the number and positions of the bar magnet sleeve 719 correspond to the number and positions of the bar magnet 718. As shown in fig. 15, during extraction, two rows of magnetic bar sleeves 719 correspond to the first row of holes and the seventh row of holes of the 96-well plate kit 91, respectively, so as to extract sixteen samples together. During detection, the first row of magnetic rod sleeves 719 sequentially pass through the first row of holes, the second row of holes, the third row of holes, the fourth row of holes, the fifth row of holes and the sixth row of holes to sequentially complete the operation of six steps. The second row of bar magnet sleeves 719 sequentially pass through the six rows of holes from the seventh row of holes, completing the six steps in sequence. Sixteen samples can be extracted at one time, and the extraction efficiency is high.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way; those skilled in the art can smoothly practice the invention as shown in the drawings and described above; however, those skilled in the art will appreciate that many modifications, adaptations, and variations of the present invention are possible in light of the above teachings without departing from the scope of the invention; meanwhile, any equivalent changes, modifications and evolution of the above embodiments according to the essential technology of the present invention still fall within the scope of the present invention.
Claims (6)
1. The full-automatic magnetic solid-phase extraction device is characterized by comprising a sample module, an information acquisition module, an X-direction driving assembly, a liquid transferring module, an extraction module, a waste module, a consumable module, a reagent module and a bottom plate; the sample module, the information acquisition module, the X-direction driving assembly and the waste module are arranged on a bottom plate; the liquid transferring module and the extraction module are respectively connected with the X-direction driving assembly; the consumable module and the reagent module are fixed on the waste module; wherein,,
the pipetting module comprises a Y-direction driving assembly, a pipetting Z-direction driving assembly and a pipetting device; the pipetting Z-direction driving assembly is fixed on the Y-direction driving assembly; the pipettor is fixed on the pipetting Z-direction driving assembly;
the extraction module is provided with a plurality of matched magnetic rod sleeves and magnetic rods, and the magnetic rod sleeves and the magnetic rods respectively move along the Z direction so as to extend into detection holes fixed on the consumable module to perform magnetic solid-phase extraction;
the X-direction driving assembly comprises a first driving mechanism and a second driving mechanism; wherein,,
the first driving mechanism comprises a first synchronous belt transmission mechanism and a first screw rod which are matched with each other; the first screw rod is matched with an extraction sliding block plate of the extraction module to form a transmission structure; the first synchronous belt transmission mechanism drives the first screw rod to rotate, so that the extraction sliding block plate drives the extraction module to move along the X direction;
The second driving mechanism comprises a second synchronous belt transmission mechanism and a second screw rod which are matched with each other; the second screw rod is matched with a pipetting slider plate of the pipetting module to form a transmission structure; the second synchronous belt transmission mechanism is used for driving the second screw rod to rotate so that the pipetting slider plate drives the pipetting module to move along the X direction;
the X-direction driving assembly further comprises at least two groups of guide components; the guide component comprises a linear guide rail, a first sliding block and a second sliding block; the linear guide rail extends along the X direction; the two first sliding blocks and the second sliding blocks are respectively and slidably connected with the linear guide rail and the extraction module and the pipetting module;
the pipetting module comprises a pipetting Z-direction connecting plate and a pipetting slider plate connected with the pipetting Z-direction connecting plate; the pipetting slide block plate is matched with the second screw rod and connected with the first slide block; the Y-direction driving assembly is arranged on the pipetting Y-direction connecting plate; the pipetting Z-direction driving assembly is arranged on the pipetting Z-direction connecting plate;
the Y-direction driving assembly comprises a third synchronous belt transmission mechanism and a pipetting Y-direction linear guide rail, and the pipetting Z-direction connecting plate is connected with the third synchronous belt transmission mechanism and is connected with the pipetting Y-direction linear guide rail in a sliding manner;
The pipetting Z-direction driving assembly comprises a fourth synchronous belt transmission mechanism, a pipetting Z-direction screw rod, a transmission sliding block and a pipetting Z-direction linear guide rail; the transmission sliding block is matched with the pipetting Z-direction screw rod to form a transmission structure; the transmission sliding block is connected with the pipetting Z-direction linear guide rail in a sliding manner and connected with the pipetting device;
the extraction module comprises an extraction sliding block plate, an extraction connecting plate, an extraction Z-direction linear guide rail, a fifth sliding block, a sixth sliding block, a fifth synchronous belt transmission mechanism, a sixth synchronous belt transmission mechanism, a magnetic rod sleeve frame and a magnetic rod frame; wherein, a plurality of the magnetic rod sleeves are fixed on the magnetic rod sleeve frame, and a plurality of the magnetic rods are fixed on the magnetic rod frame;
the extraction sliding block plate is connected to the extraction connecting plate and is matched with the first screw rod to form a transmission structure;
the fifth sliding block and the sixth sliding block are respectively and slidably connected to the extraction Z-direction linear guide rail; the fifth sliding block and the sixth sliding block are respectively connected with the magnetic rod sleeve frame and the magnetic rod frame;
the fifth synchronous belt transmission mechanism is connected with the fifth sliding block so as to drive the fifth sliding block to drive the magnetic rod sleeve frame to move along the Y direction; the sixth synchronous belt transmission mechanism is respectively connected with the sixth sliding block so as to drive the sixth sliding block to drive the magnetic rod frame to move along the Y direction.
2. The fully automated magnetic solid phase extraction device of claim 1, wherein the sample module comprises:
at least one sample rack slide rail mounted to the base plate;
at least one sample rack, which is provided with a plurality of mounting grooves for clamping the sample tubes;
the magnet mounting seat is fixed at one end of the sample rack sliding rail;
a first magnet mounted to an end of the sample holder facing the magnet mount;
a second magnet mounted to the magnet mount;
the indication assembly is used for emitting light or sound indication;
the sample frame sliding connection in the sample frame slide rail, when first magnet with the second magnet adsorbs so that sample frame end is fixed in the magnet mount pad, the instruction subassembly sends the instruction, in order to instruct the sample frame is installed in place.
3. The fully automatic magnetic solid phase extraction device according to claim 1, wherein the extraction module further comprises a baffle plate rotatably connected to the extraction slider plate or the extraction connection plate;
when extraction is carried out, the baffle rotates to be far away from the magnetic rod sleeve; after extraction is completed, the baffle rotates to be positioned below the magnetic rod sleeve so as to bear samples dripped on the surface of the magnetic rod sleeve.
4. The fully automatic magnetic solid phase extraction device according to claim 1, wherein the magnetic rod is fixed to the magnetic rod frame through a buckle structure;
and/or the magnetic rod sleeve is fixed on the magnetic rod sleeve frame through a buckle structure.
5. The fully automatic magnetic solid phase extraction device according to any one of claims 1 to 4, wherein the waste module comprises a waste feeding plate and a waste cartridge; the waste material is put in the board and is equipped with and leads to the mouth of putting in of waste material box is used for putting in the abandonment tip rifle head of pipetting module.
6. The full-automatic magnetic solid phase extraction device according to claim 5, wherein the waste module comprises a first consumable base and a second consumable base which are arranged in a staggered manner; the waste box is arranged below the first consumable base and the second consumable base; the waste throwing plate is positioned between the first consumable base and the second consumable base;
the consumable module comprises a pore plate kit and a tip fixing frame; the pore plate kit is clamped on the second consumable base; the tip fixing frame is fixed on the first consumable base through a fastener; the reagent module is fixed on the second consumable base.
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| WO2023041042A1 (en) * | 2021-09-17 | 2023-03-23 | 圣湘生物科技股份有限公司 | Nucleic acid extraction and detection device and nucleic acid extraction and detection method |
| CN115032413B (en) * | 2022-08-15 | 2023-01-03 | 杭州凯莱谱质造科技有限公司 | Sampling device for sample pretreatment and pretreatment method |
| CN116731856B (en) * | 2023-04-24 | 2023-12-01 | 扬州市职业大学(扬州开放大学) | High-flux automatic sorting device and sorting method thereof |
| CN116448535B (en) * | 2023-06-16 | 2023-11-10 | 杭州凯莱谱质造科技有限公司 | Full-automatic magnetic solid phase extraction equipment |
| CN117126727B (en) * | 2023-09-18 | 2024-04-19 | 合肥达徽基因科技有限公司 | Full-automatic extraction amplification hybridization integrated workstation |
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