CN108048302A - Full automatic instrument for extracting nucleic acid - Google Patents
Full automatic instrument for extracting nucleic acid Download PDFInfo
- Publication number
- CN108048302A CN108048302A CN201810102670.2A CN201810102670A CN108048302A CN 108048302 A CN108048302 A CN 108048302A CN 201810102670 A CN201810102670 A CN 201810102670A CN 108048302 A CN108048302 A CN 108048302A
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- nucleic acid
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- carrier
- tip
- baffle
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 75
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 75
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 75
- 238000000605 extraction Methods 0.000 claims abstract description 50
- 239000007788 liquid Substances 0.000 claims abstract description 43
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 23
- 239000000969 carrier Substances 0.000 claims abstract description 16
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229960002897 heparin Drugs 0.000 claims abstract description 11
- 229920000669 heparin Polymers 0.000 claims abstract description 11
- 239000002699 waste material Substances 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 9
- 230000033001 locomotion Effects 0.000 claims description 64
- 239000000523 sample Substances 0.000 claims description 15
- 230000007246 mechanism Effects 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 10
- 230000005540 biological transmission Effects 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 5
- 229920006324 polyoxymethylene Polymers 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 9
- 230000005291 magnetic effect Effects 0.000 description 7
- 230000008901 benefit Effects 0.000 description 6
- 239000011324 bead Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- -1 206 Substances 0.000 description 2
- 238000010241 blood sampling Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 239000004696 Poly ether ether ketone Substances 0.000 description 1
- 241000276425 Xiphophorus maculatus Species 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- JUPQTSLXMOCDHR-UHFFFAOYSA-N benzene-1,4-diol;bis(4-fluorophenyl)methanone Chemical compound OC1=CC=C(O)C=C1.C1=CC(F)=CC=C1C(=O)C1=CC=C(F)C=C1 JUPQTSLXMOCDHR-UHFFFAOYSA-N 0.000 description 1
- 239000010951 brass Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
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- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The present invention relates to a kind of Full automatic instrument for extracting nucleic acid, including the platform assembly, nucleic acid extraction component and automatic liquid relief component being arranged on bottom plate, platform assembly includes the reagent trough carrier, EP pipes carrier, heparin tube carrier, the first deep-well plates carrier and the TIP carriers that are arranged on bottom plate；The one side of platform assembly is equipped with several second deep-well plates, and nucleic acid extraction component includes can be in the second deep-well plates upper direction and the nucleic acid extraction arm that can be lifted；Automatic liquid relief component includes can be in nucleic acid extraction component and platform assembly upper direction and the liquid relief arm that can be lifted.The present invention is mainly by liquid relief part and nucleic acid extraction part, and other modules are integrated, realize the full-automatic extraction process of the nucleic acid from sample to purifying, avoid interference of the manual operation to result, reduce labor intensity, reduce consumptive material waste, improve extraction accuracy, accelerate extraction rate.
Description
Technical field
The invention belongs to Medical Devices manufacturing fields, and in particular to a kind of Full automatic instrument for extracting nucleic acid.
Background technology
Nucleic acid be by many nucleotide polymerizations into large biological molecule compound, be life one of most basic substance.Core
Acid is widely present in all animal and plant cells, in microbial body, and the in vivo nucleic acid of biology often combines to form nucleoprotein with protein.
Recently as the high speed development of molecular biology, the molecule diagnosis and detection technique based on nucleic acid are Sino-Japan in numerous areas
Benefit highlights vital effect.Nucleic acid needs needed for experiment and detection carry out purification from biological sample, but will
Multiple samples are purified or extremely difficult.This process not only needs to select suitable purification technique, but also works
Amount is also especially big, it is difficult to meet the demand that purifying is extracted to high-throughput sample currently developed rapidly.
Most instrument for extracting nucleic acid extracts nucleic acid using paramagnetic particle method at present, the core used in extraction process
Sour extraction apparatus is the instrument that the extraction work of sample nucleic acid is automatically performed using mating magnetic bead nucleic acid extracting reagent.At present
Instrument for extracting nucleic acid be divided into two classes:One kind is large-scale automatic fluid work station, and nucleic acid, advantage are obtained by transfer liquid
It is that fully automatic operation can be achieved, shortcoming is to be not exclusively suitable for nucleic acid extraction, and time-consuming longer, and each nucleic acid extraction duration is more than one
Hour；Another kind of is small-sized magnetic bar type instrument for extracting nucleic acid, by shift magnetic bead obtain nucleic acid, advantage be take it is shorter, need 30 to
50 minutes, shortcoming was need to use the matched reagent being pre-installed or scene dispenses by hand, without liquid separation and pipetting function, it is impossible to full-automatic
Handle many experiments sample.
The content of the invention
To solve the above-mentioned problems, the present invention provides it is a kind of efficiently, be quickly obtained the full-automatic of high-purity sample of nucleic acid
Instrument for extracting nucleic acid, concrete scheme are as follows：
A kind of Full automatic instrument for extracting nucleic acid, including the platform assembly, nucleic acid extraction component and automatic shifting being arranged on bottom plate
Liquid component, for the platform assembly for placing various reagents and container, the position of the reagent and container on the bottom plate can
With independent assortment, the nucleic acid extraction component is used for magnetic bead transfer type nucleic acid extraction, and the automatic liquid relief component is used for described
Accurate liquid transfer between platform assembly and the nucleic acid extraction component.
The platform assembly includes the reagent trough carrier, EP pipes carrier, heparin tube carrier, the PCR that are arranged on the bottom plate
Eight connect EP pipes carrier and TIP carriers, and the reagent trough carrier is equipped with reagent trough, and the EP pipes carrier is equipped with EP pipe supports,
The heparin tube carrier is equipped with heparin tube, and the PCR eight connects EP pipes carrier and is equipped with the companies of PCR eight EP pipes, the TIP carrier
It is equipped with TIP framves.
The nucleic acid extraction component includes 96 deep-well plates being arranged on the bottom plate, the above 96 deep-well plates
One X-axis motion, the first Z axis motion on the first X-axis motion and arranged on first Z axis transport
Nucleic acid extraction arm on motivation structure；Several magnetosheath framves, the magnetosheath frame are equipped with by the first connecting plate on the nucleic acid extraction arm
It is equipped with several vertical arrangements and the magnetosheath in 96 deep-well plates can be stretched into；The nucleic acid extraction arm is located at the magnetosheath frame
Top is equipped with several bar magnet framves by the second connecting plate, and the bar magnet frame is equipped with several bar magnets that can be stretched into the magnetosheath.
Further, the lower section of 96 deep-well plates is equipped with several heating components, and the heating component includes heat block, institute
The lower section for stating heat block is equipped with adding thermal resistance, and collet is equipped between the adding thermal resistance and the bottom plate.
Further, retaining device is additionally provided on the bottom plate, the retaining device includes being arranged in 96 deep-well plates
The baffle of side, one end of the baffle are equipped with the first rotation axis and the second rotation axis of parallel arrangement, first rotation axis away from
It is less than second rotation axis and the distance of the baffle edge with a distance from the baffle edge；First rotation axis and institute
The one end for stating the second rotation axis is connected with to drive the driving mechanism of the baffle plate turning, and the driving mechanism can drive baffle
It overturns to the surface of 96 deep-well plates.
Further, the driving mechanism includes baffle shell, and first rotation axis passes through through the baffle shell
First connecting rod is connected with the 3rd rotation axis, and second rotation axis is connected with energy through the baffle shell by the second connecting rod
Second rotation axis is enough driven to make the transmission component of circular motion；The input terminal of the transmission component is equipped with stepper motor, institute
The first movement slots offered on baffle shell for first rotation axis movement are stated, the lower section of first movement slots, which is equipped with, to be supplied
Second movement slots of the second rotation axis movement, the radian of second movement slots is more than first movement slots, when described
The height of first rotation axis described in when second rotation axis moves to the highest point of second movement slots and second rotation axis
It is identical；The baffle circuit board being connected with the stepper motor is additionally provided in the baffle shell.
The automatic liquid relief component includes the second X-axis motion being arranged on above the nucleic acid extraction component and is arranged on
The first Y-axis motion on the second X-axis motion, the liquid relief arm are arranged on the first Y-axis motion, institute
Liquid relief arm is stated equipped with several liquid relief units, each described liquid relief unit includes being arranged on the second Y-axis motion
Second Z axis motion and the probe tube being arranged on the second Z axis motion.
Further, waste gathering device is additionally provided on the bottom plate, the waste gathering device includes auxiliary and moves
Liquid arm unloads TIP devices by what discarded TIP heads were removed from the probe tube；It is described to unload being arranged right below for TIP devices
Discarded object slideway, the bottom of the discarded object slideway are equipped with storage device TIP discarded.
Further, the EP pipes carrier is equipped with the guided way of several parallel arrangements, and the bottom of the EP pipe supports is equipped with
With the card slot of guided way cooperation, the EP pipes carrier is made of polyformaldehyde material.
Further, the surrounding of the TIP carrier is coated with baffle, if the TIP carrier top surface is equipped with capable person institute
State the shrapnel that TIP framves are connected on the TIP carrier.
The beneficial effects of the present invention are：The present invention provides a kind of full-automatic nucleic acid-extracting apparatus, mainly by liquid relief portion
Divide and integrated with nucleic acid extraction part and other modules, realize the full-automatic of the nucleic acid from sample to purifying and extracted
Journey avoids interference of the manual operation to result, reduces labor intensity, reduces consumptive material waste, improves extraction accuracy, add
Fast extraction rate.
Description of the drawings
The overall structure diagram of Fig. 1 embodiment of the present invention 1,
The structure diagram of 1 platform assembly of Fig. 2 embodiment of the present invention,
The structure diagram of 1 nucleic acid extraction component of Fig. 3 embodiment of the present invention,
The structure diagram of 1 liquid-transfering device of Fig. 4 embodiment of the present invention,
The structure top view of 1 retaining device of Fig. 5 embodiment of the present invention,
The structural side view of 1 retaining device of Fig. 6 embodiment of the present invention,
The internal structure schematic diagram of 1 retaining device of Fig. 7 embodiment of the present invention,
The structure diagram of 1 heating component of Fig. 8 embodiment of the present invention,
The structure diagram of Fig. 9 1EP pipe carriers of the embodiment of the present invention,
The structure diagram of Figure 10 1TIP carriers of the embodiment of the present invention,
Figure 11 embodiment of the present invention 2 unloads the structure diagram of TIP devices.
Figure number and title：1st, bottom plate, 2, platform assembly, 201, reagent trough carrier, 202, EP pipe carriers, 203, blood sampling
Pipe carrier, 204, PCR eight connect EP pipe carriers, 205, TIP carriers, 206, reagent trough, 207, EP pipe supports, 208, heparin tube,
209th, PCR eight connects EP pipes, and 210, TIP framves, 3, nucleic acid extraction component, 301,96 deep-well plates, 302, nucleic acid extraction arm, 303, the
One X-axis motion, the 304, first Z axis motion, the 305, first connecting plate, 306, magnetosheath frame, 307, magnetosheath, 308, second
Connecting plate, 309, bar magnet frame, 310, bar magnet, 4, free liquid relief component, 401, liquid relief arm, the 402, second X-axis motion, 403,
First Y-axis motion, the 404, second Y-axis motion, the 405, second Z axis motion, 406, probe tube, 5, baffle dress
It puts, 501, baffle, 502, driving mechanism, the 503, first rotation axis, the 504, second rotation axis, 505, baffle shell, 506, first
Connecting rod, the 507, the 3rd rotation axis, the 508, second connecting rod, 509, transmission component, 510, stepper motor, the 511, first movement slots,
512nd, the second movement slots, 513, baffle circuit board, 6, waste gathering device, 601, unload TIP devices, 602, discarded object slides
Road, 7, heating component, 701, heat block, 702, adding thermal resistance, 703, collet, 8, guided way, 9, card slot, 10, baffle, 11,
Shrapnel.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and examples, and the following example is only used for explaining the present invention
The content of the invention, be not intended to limit the scope of the invention.
Embodiment 1
With reference to shown in Fig. 1 and Fig. 2, a kind of Full automatic instrument for extracting nucleic acid, including platform assembly 2, the core being arranged on bottom plate 1
Sour extraction assembly 3 and automatic liquid relief component 4, platform assembly 2 include the reagent trough carrier 201, the EP pipe carriers that are arranged on bottom plate 1
202nd, heparin tube carrier 203, PCR eight connect EP pipes carrier 204 and TIP carriers 205, and reagent trough carrier 201 is equipped with reagent trough
206, EP pipe carriers 202 are equipped with EP pipe supports 207, and heparin tube carrier 203 is equipped with heparin tube 208, and PCR eight connects EP pipe carriers
204, which are equipped with PCR eight, connects EP pipes 209, and TIP carriers 205 are equipped with TIP framves 210；Nucleic acid extraction component 3 includes several set
96 deep-well plates 301 in 2 one side of platform assembly, nucleic acid extraction component 3 further includes can be in 96 deep-well plates, 301 upper direction and energy
The nucleic acid extraction arm 302 of lifting；Automatic liquid relief component 4 include can in nucleic acid extraction component 3 and 2 upper direction of platform assembly and
The liquid relief arm 401 that can be lifted.
As shown in figure 3, nucleic acid extraction component 3 include being arranged on the first X-axis motion 303 of the top of 96 deep-well plates 301 with
And the first Z axis motion 304 on the first X-axis motion 303, nucleic acid extraction arm 302 are arranged on the movement of the first Z axis
In mechanism 304；Several magnetosheath framves 306 are equipped with by the first connecting plate 305 on nucleic acid extraction arm 302, if magnetosheath frame 306 is equipped with
It is dry to arrange vertically and the magnetosheath 307 in 96 deep-well plates 301 be stretched into；The top that nucleic acid extraction arm 302 is located at magnetosheath frame 306 passes through
Second connecting plate 308 is equipped with several bar magnet framves 309, and bar magnet frame 309 is equipped with several bar magnets 310 that can be stretched into magnetosheath 307；Magnetic
Stick frame, magnetosheath frame are divided into three groups, and every group two arranges, 8 bar magnets of each column.Compared with using a 48 big passage bar magnet framves, bar magnet
The advantages of frame is divided into three groups is flexible structure, and adjusting is facilitated to install, and reduces difficulty of processing and processing cost；Bar magnet frame, magnetosheath
Frame corner arc designs, and operating personnel during experiment is avoided to scratch hand when inserting and taking magnetosheath, reduce the infected wind of operating personnel
Danger.
Compares figure 1 and Fig. 4, automatic liquid relief component 4 include the second X-axis motion for being arranged on 3 top of nucleic acid extraction component
402 and the first Y-axis motion 403 on the second X-axis motion 402, liquid relief arm 401 is moved arranged on the first Y-axis
In mechanism 403, liquid relief arm 401 is equipped with several liquid relief units, each liquid relief unit includes being arranged on the second Y-axis motion
The second Z axis motion 405 on 404 and the probe tube 406 being arranged on the second Z axis motion 405.
With reference to Fig. 5-Fig. 7, retaining device 5 is additionally provided on bottom plate 1, retaining device 5 includes being arranged on 96 deep-well plates, 301 top
Baffle 501 and driving mechanism 502, one end of baffle 501 are equipped with the first rotation axis 503 and the second rotation axis 504 of parallel arrangement,
Distance of first rotation axis 503 apart from 501 edge of baffle is less than the second rotation axis 504 and the distance at 501 edge of baffle；Driving machine
Structure 502 includes baffle shell 505, and the first rotation axis 503 is connected with the 3rd turn through baffle shell 505 by first connecting rod 506
Moving axis 507, the second rotation axis 504 is connected with through baffle shell 505 by the second connecting rod 508 can drive the second rotation axis
504 make the transmission component 509 of circular motion；The input terminal of transmission component 509 is equipped with stepper motor 510, is opened on baffle shell 505
Equipped with the first movement slots 511 for the movement of the first rotation axis 503, the lower section of the first movement slots 511 is equipped with for the second rotation axis 504
Second movement slots 512 of movement, the radian of the second movement slots 512 is more than the first movement slots 511, when the second rotation axis 504 moves
To the second movement slots 512 highest point when the first rotation axis 503 height it is identical with the second rotation axis 504；In baffle shell 505
It is additionally provided with the baffle circuit board 513 being connected with stepper motor 510.
It is additionally provided with waste gathering device 6 on bottom plate 1, waste gathering device 6 includes auxiliary liquid relief arm 401 will be discarded
TIP removed from probe tube 406 unload TIP devices 601, as depicted in figs. 1 and 2, unload TIP devices 601 to be fixed on
Platy structure on bottom plate, top deflects to form the pawl that comes off to liquid relief arm direction, when then probe tube is close to come off pawl
Moving up can make discarded TIP heads drop；That unloads TIP devices 601 is arranged right below discarded object slideway 602, discarded object slideway
602 bottom is equipped with external dustbin TIP discarded, and discarded TIP come off are collected, avoids polluting.
Such as Fig. 8, the lower section of 96 deep-well plates 301 is equipped with several heating components 7, and heating component 7 includes heat block 701, heating
The lower section of block 701 is equipped with adding thermal resistance 702, collet 703 is equipped between adding thermal resistance 702 and bottom plate 1, each rest area includes
Independent heating unit meets during nucleic acid extraction different step to the demand for heat of reagent.Heat block is processed using brass,
Corner arc designs, and avoids operating personnel and the infected risk of gloves is scratched when taking consumptive material, built-in adding thermal resistance has heat
The advantages that conduction efficiency is fast at high speed, is spaced between heat block and platform with collet, and collet uses PEEK materials, has
Heat resistance is high, and machinability is strong, it is anticorrosive the advantages that.
As shown in figure 9, EP pipes carrier 202 is equipped with the guided way 8 of several parallel arrangements, the bottom of EP pipe supports 207 is equipped with
The card slot 9 coordinated with guided way 8 is conveniently taken using the design of drawing and pulling type slideway structure, and EP pipe supports 207 are polyformaldehyde material system
Into polyformaldehyde material has self-lubricating function, reduces the abrasion to carrier in draw process.As shown in Figure 10, TIP carriers
205 surrounding is coated with baffle 10, avoids the contaminated risk of clean TIP, if TIP 205 top surfaces of carrier are equipped with capable person
TIP framves 210 are connected to the shrapnel 11 on TIP carriers 205, effectively block steady TIP frame, make liquid relief arm when picking up TIP
TIP framves are not taken up.
Lighting system, ultraviolet sterilization system and the control system for controlling various components are also equipped on bottom plate 1
(being not drawn into attached drawing).
The present invention has both the dual function of liquid relief formula and magnetic bar type, and instrument internal includes the liquid relief of 1-8 passage independent-liftings
The high intensity bar magnet of arm and 1-48 passages, reagents slot, centrifuge tube, 96 deep-well plates, PCR eight connect the consumptive materials such as EP pipes, magnetosheath, can
The actions such as the automation transfer, suction, concussion of liquid and magnetic bead are carried out, are grasped so as to fulfill the full automation of sample preparation link
Make.
Embodiment 2
With reference to Figure 11, the present embodiment and embodiment 1 difference lies in：
The TIP devices 601 that unload of the present embodiment are to be installed on liquid relief arm to move back TIP first speed pieces, when liquid relief unit drives
Probe tube moves up, and when being moved to the position for moving back TIP first speed pieces for TIP, discarded TIP heads can automatically take off from probe tube
Fall, then fall into the discarded object slideway of lower section.Compared with embodiment 1, its course of work is more flexible.
The course of work of the present invention is as follows：
First, from preparation, by reagent, biological sample, TIP, 96 deep-well plates, PCR eight connect EP pipes etc. be placed on it is corresponding
Position；
Then, automatic pipetting device installs TIP automatically, and successively by the reagent in the reagent trough on platform module and blood sampling
Biological sample in pipe is added in the deep-well plates on nucleic acid extraction platform, automatic nucleic acid-extracting apparatus complete nucleic acid extraction and
Purifying；
Subsequently, the nucleic acid extracted is transferred to the PCR eight being placed in ice chest and connected in EP pipes by automatic pipetting device；
Finally, all waste object is collected into automatically in external dustbin, and then completes the full-automatic nucleic acid extraction of whole process.
In conclusion only the preferred embodiments of the invention, does not limit protection scope of the present invention with this, it is all according to the present invention
The equivalent changes and modifications that the scope of the claims and description are made is all within the scope of patent of the present invention covers.
Claims (10)
1. a kind of Full automatic instrument for extracting nucleic acid, it is characterised in that：Platform assembly (2), nucleic acid including being arranged on bottom plate (1) carry
Component (3) and automatic liquid relief component (4) are taken, the platform assembly is used to place various reagents and container, the reagent and container
Position on the bottom plate (1) can be freely combined, and the nucleic acid extraction component (3) is used for magnetic-type nucleic acid extraction, described
Automatic liquid relief component (4) is for the accurate liquid transfer between the platform assembly (2) and the nucleic acid extraction component (3).
2. a kind of Full automatic instrument for extracting nucleic acid according to claim 1, it is characterised in that：The platform assembly (2) includes
Reagent trough carrier (201), EP pipes carrier (202), heparin tube carrier (203), the PCR eight being arranged on the bottom plate (1) connect EP
Pipe carrier (204) and TIP carriers (205), the reagent trough carrier (201) are equipped with reagent trough (206), the EP pipes carrier
(202) EP pipe supports (207) are equipped with, the heparin tube carrier (203) is equipped with heparin tube (208), and the PCR eight connects EP pipes and carries
Frame (204) is equipped with PCR eight and connects EP pipes (209), and the TIP carrier (205) is equipped with TIP framves (210).
3. a kind of Full automatic instrument for extracting nucleic acid according to claim 1, it is characterised in that：The nucleic acid extraction component (3)
Including being arranged on 96 deep-well plates (301) on the bottom plate (1), the first X-axis fitness machine above 96 deep-well plates (301)
Structure (303), the first Z axis motion (304) on the first X-axis motion (303) and arranged on the first Z
Nucleic acid extraction arm (302) on shaft movement mechanism (304)；It is set on the nucleic acid extraction arm (302) by the first connecting plate (305)
There are several magnetosheath framves (306), the magnetosheath frame (306) is equipped with several vertical arrangements and can stretch into 96 deep-well plates (301)
Interior magnetosheath (307)；The top that the nucleic acid extraction arm (302) is located at the magnetosheath frame (306) passes through the second connecting plate (308)
Equipped with several bar magnet framves (309), the bar magnet frame (309) is equipped with several bar magnets that can be stretched into the magnetosheath (307)
(310)。
4. a kind of Full automatic instrument for extracting nucleic acid according to claim 3, it is characterised in that：It is additionally provided on the bottom plate (1)
Retaining device (5), the retaining device (5) include being arranged on the baffle (501) above 96 deep-well plates (301), the baffle
(501) one end is equipped with the first rotation axis (503) of parallel arrangement and the second rotation axis (504), first rotation axis (503)
Distance apart from the baffle (501) edge is less than second rotation axis (504) and the distance at the baffle (501) edge；
One end of first rotation axis (503) and second rotation axis (504) is connected with that the baffle (501) is driven to overturn
Driving mechanism (502), the driving mechanism (502) can be overturn to the surface of 96 deep-well plates (301).
5. a kind of Full automatic instrument for extracting nucleic acid according to claim 5, it is characterised in that：Driving mechanism (502) bag
Baffle shell (505) is included, first rotation axis (503) is connected through the baffle shell (505) by first connecting rod (506)
There is the 3rd rotation axis (507), second rotation axis (504) passes through the second connecting rod (508) through the baffle shell (505)
It is connected with the transmission component (509) that second rotation axis (504) can be driven to make circular motion；The transmission component (509)
Input terminal be equipped with stepper motor (510), offer on the baffle shell (505) and moved for first rotation axis (503)
The first movement slots (511), the lower sections of first movement slots (511) is equipped with for second rotation axis (504) movement
Two movement slots (512), the radian of second movement slots (512) are more than first movement slots (511), when the described second rotation
The height and described second of first rotation axis (503) described in when axis (504) moves to the highest point of second movement slots (512)
Rotation axis (504) is identical；The baffle circuit being electrically connected with the stepper motor (510) is additionally provided in the baffle shell (505)
Plate (513).
6. a kind of Full automatic instrument for extracting nucleic acid according to claim 1, it is characterised in that：The automatic liquid relief component (4)
It is moved including the second X-axis motion (402) being arranged on above the nucleic acid extraction component (3) and arranged on second X-axis
The first Y-axis motion (403) in mechanism (402), the liquid relief arm (401) are arranged on the first Y-axis motion (403)
On, the liquid relief arm (401) is equipped with several liquid relief units, each described liquid relief unit includes the second Y-axis motion
(404), the second Z axis motion (405) on the second Y-axis motion (404) and it is arranged at the 2nd Z
Probe tube (406) on shaft movement mechanism (405).
7. a kind of Full automatic instrument for extracting nucleic acid according to claim 2, it is characterised in that：It is additionally provided on the bottom plate (1)
Waste gathering device (6), the waste gathering device (6) include auxiliary liquid relief arm (401) by discarded TIP heads from described
What is removed on probe tube (406) unloads TIP devices (601)；The discarded object that is arranged right below for unloading TIP devices (601) is slided
Road (602), the bottom of the discarded object slideway (602) are equipped with storage device TIP discarded.
8. a kind of Full automatic instrument for extracting nucleic acid according to claim 2, it is characterised in that：96 deep-well plates (301)
Lower section is equipped with several heating components (7), and the heating component (7) includes heat block (701), the lower section of the heat block (701)
Equipped with adding thermal resistance (702), collet (703) is equipped between the adding thermal resistance (702) and the bottom plate (1).
9. a kind of Full automatic instrument for extracting nucleic acid according to claim 2, it is characterised in that：On the EP pipes carrier (202)
Guided way (8) equipped with several parallel arrangements, the bottom of the EP pipe supports (207) are equipped with the card with the guided way (8) cooperation
Slot (9), the EP pipe supports (207) are made of polyformaldehyde material.
10. a kind of Full automatic instrument for extracting nucleic acid according to claim 2, it is characterised in that：The TIP carrier (205)
Surrounding be coated with baffle (10), the TIP carrier (205) is if top surface is connected to institute equipped with TIP framves (210) described in capable person
State the shrapnel (11) on TIP carriers (205).
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| CN201810102670.2A CN108048302B (en) | 2018-02-01 | 2018-02-01 | Full-automatic nucleic acid extractor |
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| CN201810102670.2A CN108048302B (en) | 2018-02-01 | 2018-02-01 | Full-automatic nucleic acid extractor |
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| CN108048302B CN108048302B (en) | 2024-03-19 |
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