CN114480104A - Nucleic acid extraction device - Google Patents
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- 238000000605 extraction Methods 0.000 title claims abstract description 112
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 91
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 91
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 91
- 238000006243 chemical reaction Methods 0.000 claims abstract description 77
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 73
- 238000003908 quality control method Methods 0.000 claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 230000007246 mechanism Effects 0.000 claims description 77
- 239000011324 bead Substances 0.000 claims description 29
- 239000002904 solvent Substances 0.000 claims description 22
- 238000012546 transfer Methods 0.000 claims description 16
- 238000005057 refrigeration Methods 0.000 claims description 13
- 239000003381 stabilizer Substances 0.000 claims description 9
- 238000006073 displacement reaction Methods 0.000 claims description 7
- 238000001514 detection method Methods 0.000 abstract description 26
- 238000000034 method Methods 0.000 abstract description 22
- 230000008569 process Effects 0.000 abstract description 19
- 230000003321 amplification Effects 0.000 abstract description 7
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 7
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 10
- 239000000126 substance Substances 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 238000010438 heat treatment Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
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- 230000035484 reaction time Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention provides a nucleic acid extraction device, comprising: the device comprises a sample module, a reagent module, a liquid-transferring module, a reaction module, a temperature control module and an extraction module, wherein a sample is accommodated in the sample module; a plurality of reagents are contained in the reagent module; the pipetting module is used for extracting and transferring liquid to be pipetted, and the liquid to be pipetted comprises a sample and a reagent; the reaction module comprises a reaction platform, and the reaction platform is used for reacting a sample with a reagent; the temperature control module is used for controlling the temperature of the reaction platform; the extraction module is used for extracting the reacted sample. After the nucleic acid extraction device provided by the invention is used for placing a sample, the operations of sample liquid transferring, reagent adding, amplification and extraction can be automatically carried out, the contact between detection personnel and the sample, a positive quality control product and the like is avoided as much as possible, and the possibility of infection of the detection personnel in the detection process is reduced. The nucleic acid extraction device performs automated nucleic acid extraction operation, and requires less labor for detection and high efficiency.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a nucleic acid extraction device.
Background
How to rapidly and effectively extract effective nucleic acid of a detection object in the pretreatment process is a key environment for detecting various drug-resistant bacteria, pathogenic bacteria, viruses and the like. The prior pretreatment method mainly comprises the steps of sampling, nucleic acid extraction and detection reagent addition, the whole operation process adopts manual one-by-one recording and one-by-one matching, the whole operation process is time-consuming and labor-consuming, and the problem of operation sample pollution is possibly caused. Moreover, in the manual operation, the operator is likely to contact the specimen and become infected.
When a large number of detection objects need to be processed, a large amount of time and labor are consumed for preprocessing, and the detection period is long due to low efficiency. In addition, in the prior art, information matching is troublesome in the preprocessing process, which easily causes the problems of information error, irretraceable detection result and the like.
Disclosure of Invention
In view of the above, in order to overcome the above-mentioned drawbacks of the prior art, the present invention proposes a nucleic acid extraction device for performing automated nucleic acid extraction.
The nucleic acid extraction device includes:
the sample module comprises a first rotating disc, a plurality of clamping grooves are formed in the first rotating disc, the clamping grooves are used for accommodating sample adapters, the sample adapters are used for accommodating sample tubes, and samples are accommodated in the sample tubes;
a reagent module comprising a plurality of solvent bins containing a plurality of reagents;
a pipetting module for extracting and transferring a liquid to be pipetted, the liquid to be pipetted comprising the sample and the reagent;
a reaction module comprising a reaction platform for reacting the sample and the reagent;
the temperature control module is used for controlling the temperature of the reaction platform;
an extraction module for extracting the reacted sample.
The sample adapter comprises a main body and an upper cover, wherein the main body is used for accommodating the sample tubes, and the upper cover is used for covering the sample tubes;
the sample module further comprises an uncovering mechanism and a tube-releasing mechanism, the uncovering mechanism is used for opening an upper cover of the sample adapter, and the tube-releasing mechanism is used for guiding the sample adapter to move so that the sample adapter moves out of the clamping groove. When a sample is placed by a detection person, only the sealed sample tube needs to be contacted, the nucleic acid extraction device automatically uncovers and takes off the sample adapter, and the contact between the detection person and the sample is avoided.
The liquid transfer module comprises a liquid transfer gun head, a liquid transfer arm and a moving unit, the moving unit is connected with the liquid transfer arm and used for driving the liquid transfer arm to move, an installation mechanism is arranged on the liquid transfer arm and used for installing the liquid transfer gun head, and the liquid transfer gun head is used for extracting and releasing liquid to be transferred.
The moving unit comprises a first moving assembly and a second moving assembly, the first moving assembly drives the liquid transfer arm to move on the horizontal plane, and the second moving assembly drives the liquid transfer arm to move up and down. The pipetting module can realize free and accurate movement inside the nucleic acid extraction device to complete pipetting operation.
The reaction module further comprises a displacement component, and the displacement component is used for driving the reaction platform to move to a first preset position in the direction close to the liquid transferring module or driving the reaction platform to move to a second preset position in the direction far away from the liquid transferring module;
the reaction platform is used for supplying the liquid transferring module to transfer the liquid to be transferred when moving to the first preset position, and the extraction module is positioned above the reaction platform when moving to the second preset position.
The extraction module comprises an extraction unit and a lifting mechanism, and the extraction unit is connected with the lifting mechanism;
the extraction unit is used for extracting the reacted sample, and the lifting mechanism is used for driving the extraction unit to ascend or descend.
In some embodiments, the nucleic acid is extracted by a magnetic bead method, the nucleic acid extraction device further comprises a magnetic bead tube and a magnetic attraction system, and the extraction module comprises a magnetic rod;
magnetic beads are contained in the magnetic bead tubes, the magnetic attraction system is used for sucking the magnetic beads from the magnetic bead tubes and adding the magnetic beads to the reaction platform, and the magnetic bars are used for sucking the magnetic beads in the reaction platform.
Further, the nucleic acid extraction device also comprises a refrigeration module, and a stabilizer is arranged in the refrigeration module.
In some embodiments, the nucleic acid extraction device further comprises a quality control module containing a quality control standard and a control module comprising a plurality of culture units for containing the reacted sample and the quality control standard.
The nucleic acid extraction device further comprises a scanning module for acquiring information of the sample. The scanning module is used for acquiring the information of the sample which is extracted currently, so that the information of the sample is accurate and the detection result can be traced.
In summary, the nucleic acid extraction device of the present invention has the following beneficial effects: carry out the nucleic acid extraction operation through the machine is automatic, and the testing personnel only need contact required consumptive material in sealed sample tube, the testing process, avoided the contact between testing personnel and sample, positive quality control article etc. as far as possible, reduced the possibility that testing personnel infected in the testing process.
The automated nucleic acid extraction operation requires less labor and high efficiency for detection.
The nucleic acid extraction device records and matches sample information in the process of nucleic acid extraction, and can ensure that the sample is matched with the sample information, so that the information of the sample after nucleic acid extraction is accurate and the detection result can be traced.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic view of the overall configuration of a nucleic acid extraction apparatus according to the present invention;
FIG. 2 is a schematic view of another angle of the overall structure of the nucleic acid extracting apparatus of the present invention;
FIG. 3 is a schematic view of a partial structure of the nucleic acid isolation apparatus according to the present invention;
FIG. 4 is a schematic view showing the construction of a reagent module of the nucleic acid isolation apparatus according to the present invention;
FIG. 5 is a schematic view showing the structure of an extraction module of the nucleic acid extraction apparatus of the present invention;
FIG. 6 is a schematic view showing the structure of a sample block of the nucleic acid isolation apparatus according to the present invention;
FIG. 7 is a schematic view of a partial structure of a sample module;
FIG. 8 is a schematic structural view of a uncapping block;
FIG. 9 is a schematic structural view of a pipe-removing block;
FIG. 10 is a schematic diagram of a sample adapter for receiving a sample tube;
FIG. 11 is a schematic view of the structure of the sample module and the scanning module assembled together;
FIG. 12 is a schematic view of a multi-station tube housing;
FIG. 13 is a schematic view of another configuration of a multi-station ferrule cartridge;
FIG. 14 is a schematic view of a structure of a tube housing accommodating a reagent tube.
Reference numerals:
1-a sample module; 11-a first carousel; 12-a card slot; 13-a sample adapter; 14-sample tube; 15-a body; 16-an upper cover; 17-uncapping block; 171-a first bevel; 18-a tube removal block; 181-a second inclined plane; 19-a first drive mechanism; 2-a reagent module; 21-a solvent bin; 22-an overflow trough; 3-pipetting module; 31-pipette tips; 32-a pipetting arm; 33-a lance tip box unit; 4-a reaction module; 41-reaction platform; 42-a displacement assembly; 5-an extraction module; 51-an extraction unit; 52-a lifting mechanism; 53-magnetic bead tube; 6-a scanning module; 7-a refrigeration module; 71-a cabin body; 72-a cover body; 73-a second carousel; 74-accommodating grooves; 740-a pipe sleeve structure; 75-a second drive mechanism; 76-a sleeve body; 77-tube cover; 78-a cap screwing mechanism; 79-reagent tube; 8-quality control product module; 81-a stop structure; 82-a through hole; 83-a guide structure; 9-a control module; 10-operation panel.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a nucleic acid extraction device, which is used for carrying out automatic nucleic acid extraction operation.
Example 1
Referring to fig. 1 to 3, the nucleic acid extraction apparatus includes a sample module 1, a reagent module 2, a pipetting module 3, a reaction module 4, a temperature control module, and an extraction module 5. The sample module 1 comprises a first rotary disc 11, a plurality of clamping grooves 12 are arranged on the first rotary disc 11, the clamping grooves 12 are used for accommodating sample adapters 13, the sample adapters 13 are used for accommodating sample tubes 14, and samples are accommodated in the sample tubes 14. The reagent module 2 includes a plurality of solvent silos containing a plurality of reagents. The pipetting module 3 is used for extracting and transferring the liquid to be pipetted, which comprises a sample and a reagent. The reaction module 4 includes a reaction platform 41, and the reaction platform 41 is used for reacting the sample and the reagent. The temperature control module is used for controlling the temperature of the reaction platform 41. The extraction module 5 is used for extracting a sample after reaction.
When the nucleic acid extraction device provided by the invention is used for carrying out nucleic acid extraction operation, the sample tube 14 containing a sample is sealed by the sample adapter 13 and then arranged on the sample module 1. The sample is extracted by the pipetting module 3 and then transferred to the reaction platform 41. Then, the reagent is extracted from the reagent module 2 by the pipetting module 3 and transferred to the reaction platform 41, and the sample reacts with the reagent at the reaction platform 41. The temperature control module controls the temperature of the reaction platform 41 when the sample and the reagent are amplified in the reaction platform 41, so that the temperature change in the reaction platform 41 meets the temperature change requirement for nucleic acid amplification. After the reaction between the sample and the reagent is completed on the reaction platform 41, the reacted sample is extracted by the extraction module 5, and the reacted sample extracted by the extraction module 5 is used for nucleic acid detection.
Specifically, referring to fig. 4 of the specification, in the present embodiment, a solvent storage device is connected to the plurality of solvent bins 21 of the reagent module 2, the capacity of the solvent storage device is much larger than that of the solvent bins 21, and a pump is disposed between the solvent bins 21 and the solvent storage device. Be provided with first liquid level detection device and second liquid level detection device in solvent storehouse 21, first liquid level detection device sets up and is used for detecting whether empty in the solvent storehouse 21 in the bottom of solvent storehouse 21, and second liquid level detection device sets up and is used for detecting whether full in solvent storehouse 21 at solvent storehouse 21 top. When the first liquid level detection device detects that the solvent bin 21 is empty, the reagent in the solvent storage device is pumped into the solvent bin 21 through the pump until the second liquid level detection device detects that the solvent bin 21 is full. The solvent bin 21 is provided with an overflow groove 22 at its side, and when the liquid level of the reagent in the solvent bin 21 is higher than a predetermined height, the reagent will flow into the overflow groove 22 to prevent the reagents in different solvent bins 21 from being contaminated.
The pipetting module 3 comprises a pipetting head 31, a pipetting arm 32 and a moving unit, the moving unit is connected with the pipetting arm 32 and used for driving the pipetting arm 32 to move, a mounting structure is arranged on the pipetting arm 32 and used for mounting the pipetting head 31, and the pipetting head 31 is used for extracting and releasing liquid to be pipetted. Further, the pipetting module 3 further comprises a tip magazine unit 33 and a tip doffing unit, the tip magazine unit 33 being configured to accommodate the pipette tips 31.
Before extracting a sample through the pipetting module 3, the pipetting arm 32 is controlled to move to the tip box unit 33 through the moving unit, the pipetting tip 31 is installed on the pipetting arm 32, then the pipetting arm 32 is driven to rotate to the sample module 1 for sample extraction, and after the extracted sample is transferred to the reaction platform 41, the pipetting arm 32 is driven to rotate to the tip removing unit to remove the pipetting tip 31. Before the next sample extraction, the pipette arm 32 is driven again to move to the tip magazine unit 33 to mount a new pipette tip 31, thereby preventing sample contamination.
Before extracting the reagent through the pipetting module 3, the pipetting arm 32 is controlled to move to the tip box unit 33 through the moving unit, the pipetting tip 31 is installed on the pipetting arm 32, then the pipetting arm 32 is driven to rotate to the reagent module 2 to extract the reagent, the extracted reagent is transferred to the reaction platform 41, and then the pipetting arm 32 is driven to rotate to the tip removing unit to remove the pipetting tip 31. Before the next reagent extraction, the pipette arm 32 is driven again to move to the tip magazine unit 33 to mount a new pipette tip 31, thereby preventing the reagent from being contaminated.
The moving unit comprises a first moving component and a second moving component, the first moving component drives the pipetting arm 32 to move on a horizontal plane so that the free end of the pipetting arm 32 can move to the upper part of different modules, and the second moving component drives the pipetting tip 31 to move up and down so that the pipetting arm 32 can extend into different modules for operation.
For example, a mounting structure is provided on the free end of the pipetting arm 32, the first moving component drives the pipetting arm 32 to move so that the free end moves above the tip box unit 33, the second moving component drives the pipetting arm 32 to move downwards to mount the pipetting tip 31, and the second moving component drives the pipetting arm 32 to move upwards after the mounting is completed. Next, the first moving component drives the liquid-transferring arm 32 to move so that the free end moves to the upper side of the sample module 1, then the second moving component drives the liquid-transferring arm 32 to move downwards to extract the sample, and the second moving component drives the liquid-transferring arm 32 to move upwards after the sample is extracted. Next, the first moving component drives the liquid-transferring arm 32 to move so that the free end moves above the reaction platform 41, then the second moving component drives the liquid-transferring arm 32 to move downwards to release the sample extracted from the sample module 1 into the reaction platform 41, and the second moving component drives the liquid-transferring arm 32 to move upwards after the sample is released. Then the first moving component drives the pipetting arm 32 to move, so that the free end moves to the pipette tip removing unit to remove the pipette tip 31. And completing sample pipetting operation once according to the operation flow. In the same way, reagent pipetting operation can be realized. The operation flow can be circularly executed to finish multiple sample pipetting operations and multiple reagent pipetting operations.
The reaction platform 41 is provided with a plurality of reaction units, and each time a sample pipetting operation transfers a different sample to a different reaction unit, one reaction unit accommodates one sample. When reagent pipetting is performed, a reagent for reacting with a sample is transferred to a containing unit containing the corresponding sample.
After the transfer of the sample and the reagent is completed, the sample reacts with the reagent in the reaction platform 41. In the process of reacting the sample with the reagent, the temperature control module controls the temperature of the reaction platform 41 so that the temperature change of the reaction platform 41 in the process of reacting the sample with the reagent meets the temperature change requirement for nucleic acid amplification. In particular, the temperature control module may comprise a heating plate disposed below the reaction platform 41, and the temperature generated by the heating plate can be conducted to the reaction platform 41. In the process of reacting the primary sample with the reagent, the heating plate is controlled to be heated to a preset temperature, then the heating plate is closed after a preset time, so that the reaction platform 41 is naturally cooled, and the amplification reaction of the sample and the reagent is completed after the preset reaction time.
The reaction module 4 further comprises a displacement assembly 42, and the displacement assembly 42 is configured to drive the reaction platform 41 to move to a first preset position in a direction close to the pipetting module 3 or drive the reaction platform 41 to move to a second preset position in a direction away from the pipetting module 3. When the reaction platform 41 moves to the first preset position, the liquid transfer module 3 is used for transferring the liquid to be transferred, and when the reaction platform 41 moves to the second preset position, the extraction module 5 is located above the reaction platform 41. The extraction module 5 comprises an extraction unit 51 and a lifting mechanism 52, wherein the extraction unit 51 is connected with the lifting mechanism 52. The extraction unit 51 is used for extracting a reacted sample, and the lifting mechanism 52 is used for driving the extraction unit 51 to ascend or descend.
After the amplification reaction between the sample and the reagent is completed, the displacement assembly 42 drives the reaction platform 41 to move to the second predetermined position. The lifting mechanism 52 drives the extraction unit 51 to descend, the extraction unit 51 extracts the reacted sample, and the lifting mechanism 52 drives the extraction unit 51 to ascend after the extraction is finished.
In this embodiment, the magnetic bead method is used to extract nucleic acid, so in this embodiment, the nucleic acid extraction apparatus further comprises a magnetic bead tube 53 and a magnetic attraction system, and the extraction unit 51 is a magnetic rod. Magnetic beads are contained in the magnetic bead tube 53, the magnetic attraction system is used for attracting magnetic beads from the magnetic bead tube 53 and adding magnetic beads to the reaction platform 41, and the magnetic rod is used for attracting magnetic beads in the reaction platform 41. The specific process is as follows: after the amplification reaction of the sample and the reagent is completed, the magnetic attraction system attracts the magnetic beads from the magnetic bead tube 53 and adds the attracted magnetic beads to the reaction platform 41. The magnetic beads are combined with nucleic acid molecules in the reaction platform 41, and after a preset time, the lifting mechanism 52 drives the magnetic rod to descend, and the magnetic rod absorbs the magnetic beads combined with the nucleic acid molecules to complete nucleic acid extraction.
In this embodiment, a 96-well plate is disposed on the reaction platform 41 to form a plurality of reaction units, and after the extraction module 5 completes the nucleic acid extraction, the method further includes: the original 96-well plate on the reaction platform 41 is removed and a new 96-well plate is added to the reaction platform 41.
Example 2
On the basis of embodiment 1, this embodiment 1 provides a specific example of automatically uncapping and uncapping the sample adapter 13.
Referring to fig. 5-10, in the present embodiment, the sample adapter 13 includes a main body 15 and an upper cover 16, the main body 15 is used for accommodating the sample tube 14, and the upper cover 16 is used for covering the sample tube 14. The sample module 1 includes an uncovering mechanism for opening the upper cover 16 of the sample adapter 13 and a tube-releasing mechanism for guiding the movement of the sample adapter 13 to move the sample adapter 13 out of the card slot 12.
In this embodiment, the card slot 12 is disposed at an outer edge of the first rotating disk 11, one end of the card slot 12 is an opening, and a protruding structure for engaging with the first rotating disk 11 is disposed on a side wall of the sample adapter 13, so that the sample adapter 13 is supported on the first rotating disk 11. The sample adapter 13 is attached to the first turntable 11 by sliding the sample adapter 13 into the card slot 12 from the opening of the card slot 12, and the sample adapter 13 can be detached from the first turntable 11 by sliding the sample adapter 13 in the direction of the opening of the card slot 12.
The uncapping mechanism comprises an uncapping block 17, the uncapping block 17 is arranged above the first rotary disc 11, and a lug is arranged on the upper cover 16 of the sample adapter 13, and the upper cover 16 can be conveniently pushed to move through the lug. The height of the lower end surface of the uncapping block 17 is lower than that of the upper end surface of the lug, and the second driving mechanism 75 drives the first rotating disc 11 to rotate, so that the lug on the upper cover 16 of the sample adapter 13 is contacted with the uncapping block 17 when the sample adapter 13 rotates to the position corresponding to the uncapping block 17.
The uncapping block 17 has a first inclined surface 171 formed on a side surface thereof, and the first inclined surface 171 guides the movement of the upper cover 16. The sample adapter 13 moves to the uncapping mechanism to complete uncapping, and then stops moving after moving to the preset area, that is, the second driving mechanism 75 stops moving after moving to the position where the nozzle of the sample tube 14 is not blocked, so that the uncapped sample tube 14 stays in the preset area to extract a sample.
Referring to FIG. 11, the nucleic acid extracting apparatus preferably further comprises a scanning module 6, and the scanning module 6 is used for obtaining information of the sample. Specifically, the sample adapter 13 or the sample tube 14 is provided with an identification code containing information of a sample in the sample tube 14, and the scanning mechanism is arranged corresponding to the preset area and used for scanning the identification code to acquire information of the sample when the sample adapter 13 stays in the preset area to extract the sample, so that the sampling device can ensure that the sample is matched with the information of the sample in the process of extracting nucleic acid from the sample, and the information of the sample after extracting nucleic acid is accurate and the detection result can be traced.
The tube-removing mechanism is used to guide the movement of the sample adapter 13 to move the sample adapter 13 out of the card slot 12 after the operation of extracting the sample in the sample tube 14 is completed. In this embodiment, the first rotating disk 11 is connected with a first driving mechanism 19 to drive the first rotating disk 11 to rotate, the first driving mechanism 19 drives the first rotating disk 11 to rotate clockwise, and the tube removing mechanism is arranged on the left side of the cap opening mechanism, so that the sample adapter 13 is opened by the cap opening mechanism during the movement process and then moves to the tube removing mechanism to remove tubes.
The tube removing mechanism comprises a tube removing block 18, a second inclined surface 181 is formed on one side of the tube removing block 18 facing the cap opening mechanism, and the second inclined surface 181 is used for guiding the main body 15 of the sample adapter 13 to move towards the outer edge direction of the first rotary disc 11. The second driving mechanism 75 drives the first rotating disk 11 to rotate, when the main body 15 of the sample adapter 13 contacts the second inclined surface 181, the main body 15 of the sample adapter 13 moves along the second inclined surface 181 to the outer edge of the first rotating disk 11 under the guidance of the second inclined surface 181, so as to move the main body 15 of the sample adapter 13 out of the card slot 12.
Further, the sampling device can further comprise a slide way mechanism, the slide way mechanism comprises a slide way part and a collecting part, and the slide way mechanism is arranged below the cover opening mechanism and/or the pipe removing mechanism. One end of the slide way part is connected with the lower part of the cover opening mechanism and/or the tube removing mechanism, the other end of the slide way part is connected with the collecting part, and the upper cover 16 of the sample adapter 13 after the cover is opened and/or the main body 15 of the sample adapter 13 after the tube is removed and the sample tubes 14 contained in the main body 15 all slide into the collecting part through the slide way part to be collected so as to be convenient for processing.
As a preferable scheme, the chute mechanism includes a first chute, a second chute, a first collecting tank, and a second collecting tank. The first slide way is arranged below the cover opening mechanism, one end of the first slide way is connected with the cover opening mechanism, the other end of the first slide way is connected with the first collecting tank, after the cover opening mechanism finishes opening the cover, the upper cover 16 of the sample adapter 13 slides out to the first collecting tank through the first slide way, and the waste upper cover 16 can be automatically collected so as to be convenient for processing. The second slide is arranged below the tube removing mechanism, one end of the second slide is connected with the tube removing mechanism, the other end of the second slide is connected with the second collecting tank, after the tube removing mechanism finishes tube removing, the main body 15 of the sample adapter 13 and the sample tubes 14 contained in the main body 15 slide out to the second collecting tank through the second slide, and the main body 15 and the sample tubes 14 of the waste sample adapter 13 can be automatically collected so as to be convenient for processing.
Other contents identical to those of embodiment 1 are not described herein again.
Example 3
In addition to example 1, the nucleic acid isolation apparatus provided in this example further has a quality control mode, and can realize a quality control function.
Specifically, nucleic acid extraction element still includes cold-stored module 7, quality control article module 8 and contrast module 9, is provided with the stabilizer in the cold-stored module 7, has held the quality control standard substance in the quality control article module 8, and contrast module 9 includes a plurality of cultivation units, and a plurality of cultivation units are used for holding sample and the quality control standard substance after the reaction.
Under the quality control mode, the nucleic acid extraction device firstly adds the stabilizing agent in the refrigeration module 7 to the culture unit of the contrast module 9 through the liquid transfer module 3, and then adds the quality control standard substance in the quality control module 8 and the sample after the amplification reaction in the reaction module 4 to different culture units in the contrast module 9. The quality control standard and the sample are cultured under the same culture conditions in the control module 9 to perform control.
In some embodiments, eight tubes are provided in the control module 9 to form a plurality of culture units.
The setting of the quality control standard needs to avoid polluting samples, reagents and the like in other structures, and different quality control products need to be prevented from being polluted when a plurality of quality control standards are set. The stabilizer needs to be placed under refrigeration, which makes the storage requirement of the stabilizer different from that of the sample, reagent, and the like in other structures in the nucleic acid extraction device. The requirement for the arrangement of the quality control module 8 and the refrigerating module 7 as described above is a major problem in adding the quality control module 8 and the refrigerating module 7 to the apparatus for difficult nucleic acid extraction to realize the quality control function. In order to solve the above problems, the present embodiment provides a multi-station tube sleeve bin structure.
Referring to fig. 12-14, the multi-station pipe sleeve bin comprises a bin body 71 and a cover body 72, wherein the cover body 72 covers an opening of the bin body 71, a second rotating disc 73 is arranged in the bin body 71, an accommodating groove 74 is arranged on the second rotating disc 73, and the accommodating groove 74 is used for accommodating a pipe sleeve structure 740. The second rotary disk 73 is connected with a second driving mechanism 75, and the second driving mechanism 75 is used for driving the second rotary disk 73 to rotate. The tube housing structure 740 includes a tube housing main body 76, a tube cover 77, and a cap screwing mechanism 78, the tube housing main body 76 is used for accommodating the reagent tube 79, one end of the cap screwing mechanism 78 is rotatably connected to the main body 15, the other end of the cap screwing mechanism 78 is connected to the tube cover 77, and the tube cover 77 is used for covering the reagent tube 79. Every reagent pipe 79 that sets up on the multistation pipe box storehouse can both cover alone and close sealedly, avoids influencing other reagent pipe 79 when operating one reagent pipe 79. When the multi-station tube sleeve bin is arranged in the nucleic acid extraction device, the nucleic acid can only enter the multi-station tube sleeve bin through the through hole 82 for extraction operation, and the rest part is of a closed structure.
The inner wall of the bin body 71 is provided with a stopping structure 81, the stopping structure 81 is used for guiding the cover screwing mechanism 78 to open the tube cover 77, the cover body 72 is provided with a through hole 82, the through hole 82 corresponds to the stopping structure 81, and when the tube cover 77 of the tube sleeve structure 740 is opened, the tube opening of the reagent tube 79 is exposed from the through hole 82. The pipetting module 3 can extract the quality control standard substance, the stabilizer and the like in the reagent tube 79 with the tube cover 77 in the open state through the through hole 82, and the other reagent tubes 79 are in the closed state, so that the influence on the other reagent tubes 79 in the operation process can be avoided.
In order to ensure that the pipe sleeve structure 740 tightly covers the reagent pipe 79, the inner wall of the bin body 71 is provided with a guide structure 83, and the guide structure 83 is used for guiding the cap screwing mechanism 78 to cover the pipe cap 77 so that the pipe cap 77 tightly covers the pipe orifice of the reagent pipe 79.
Specifically, the stopping structure 81 and the guiding structure 83 are both arranged along the circumferential direction of the inner wall of the bin body 71, and a cambered surface is arranged on the stopping structure 81 and used for guiding the cap screwing mechanism 78 of the pipe sleeve structure 740 to rotate so as to open the pipe cap 77. In the horizontal direction, the guiding structure 83 is provided with a slope toward both ends of the stopping structure 81, and the slope is used for guiding the cap screwing mechanism 78 to rotate so as to cover the tube cap 77. The lower end surface of the cap screwing mechanism 78 of the tube cover structure 740 abuts against the guide structure 83, and the guide structure 83 causes the tube cover 77 of the tube cover structure 740 to cover the reagent tube 79. When the second driving mechanism 75 drives the second rotating disc 73 to rotate so that the pipe sleeve structure 740 rotates to the position where the stopping structure 81 is arranged, the lower end surface of the cap screwing mechanism 78 is separated from the guiding structure 83, the upper end surface of the cap screwing mechanism 78 abuts against the cambered surface of the stopping structure 81 and moves along the cambered surface of the stopping structure 81 in the rotating process, and the cap screwing mechanism 78 is rotated so as to open the pipe cap 77. The capping mechanism 78 rotates to a maximum opening angle when the capping mechanism 78 moves to the lowest point of the stop structure 81. The second turntable 73 continues to rotate, the cap screwing mechanism 78 gradually rotates towards the capping direction, and when the lower end surface of the rotating mechanism contacts with the inclined surface of the guiding structure 83, the cap screwing mechanism 78 moves along the inclined surface of the guiding structure 83, so that the cap screwing mechanism 78 rotates to the tube cap 77 to cap the reagent tube 79.
In this embodiment, the quality control product module 8 includes the above-mentioned multistation pipe sleeve storehouse, and the reagent pipe 79 in the multistation pipe sleeve storehouse is used for holding the quality control standard substance, places the quality control standard substance alone in the multistation pipe sleeve storehouse and can avoid the quality control standard substance to pollute sample, reagent etc. in other structures, and can avoid placing the mutual pollution of quality control product in different reagent pipes 79. Optionally, the quality control module 8 may be set according to actual requirements, and may include a positive quality control unit or a negative quality control unit, or may include a positive quality control unit and a negative quality control unit.
The refrigeration module 7 comprises the multi-station pipe sleeve bin and a refrigeration assembly, the refrigeration assembly comprises a refrigerator and a refrigeration switch, the refrigerator is arranged in the bin body 71 and electrically connected with the refrigeration switch, and the refrigeration switch is used for controlling the refrigerator to be opened and closed. Reagent pipe 79 in the multistation pipe box storehouse of cold-stored module 7 is used for holding the stabilizer, can form cold-stored environment in the cold-stored module 7, accords with the demand of depositing of stabilizer, and can not exert an influence to the environment of depositing like sample module 1, reagent module 2 isotructure.
Other parts that are the same as those in embodiment 1 will not be described herein again.
Example 4
The nucleic acid extraction device that this embodiment provided includes control module, and control module connects sample module 1, reagent module 2, moves liquid module 3, reaction module 4, temperature control module, draws module 5, cold-stored module 7, quality control article module 8 and contrast module 9, and the function that control module realized includes: the state of each module connected with the nucleic acid extracting device is obtained, the operation flow of each module connected with the nucleic acid extracting device is set, the number of consumables such as pipette tips 31, 96 orifice plates, octa-tubes and the like arranged in the nucleic acid extracting device is stored, and the change of the number of the consumables is recorded according to the operation flow of the nucleic acid extracting device.
The control module is provided with an operation panel 10, and the operation panel 10 is used for displaying the states of the modules in the nucleic acid extraction device, which are identified by the control module, including the running states of the modules, the residual amount of consumables arranged in the modules and the like. The tester can obtain the relevant information of each module in the nucleic acid extraction device through the operation panel 10, so as to supplement the consumable in time.
The detection personnel can also set the operation sequence of each module of the nucleic acid extraction device through the operation panel 10, and set the data such as the reaction time of the reaction platform 41, the temperature change adjustment of the temperature control module and the like, and the operation of each module of the nucleic acid extraction device can be carried out according to a preset flow, and can also be carried out according to the user-defined setting of the detection personnel to form a new operation flow.
The control module may store a plurality of operation flows, for example, a flow executed in a normal mode for performing a normal nucleic acid extraction operation, a flow executed in a quality control mode for performing the nucleic acid extraction apparatus, and the like, and may acquire stored flow information and select an application via the control panel.
In some embodiments, a sterilization module is further arranged in the nucleic acid extraction device, and the sterilization module is connected with the control module. For example, the sterilization module includes an ultraviolet lamp and the control module is configured to control the ultraviolet lamp to be turned on and off. Preferably, the control module can be used for customizing the opening time and the closing time of the sterilization module.
In some embodiments, the nucleic acid extraction device further comprises a blower for providing directional, stable ventilation of the gas flow to the internal environment of the nucleic acid extraction device. If a fan is arranged to pump out the air in the nucleic acid extraction device, a negative pressure environment is formed in the nucleic acid extraction device.
In summary, the nucleic acid extraction device provided by the present invention can automatically perform nucleic acid extraction operations, requires less labor for detection, is highly efficient, and can perform rapid and accurate large-scale nucleic acid extraction operations.
Carry out the nucleic acid extraction operation through the machine is automatic, and the testing personnel only need contact required consumptive material in sealed sample tube, the testing process, avoided the contact between testing personnel and sample, positive quality control article etc. as far as possible, reduced the possibility that testing personnel infected in the testing process. Furthermore, the contact between the sample, the positive quality control product and the like and the environment is reduced, and the pollution to the environment is avoided.
The nucleic acid extraction device records and matches sample information in the process of nucleic acid extraction, and can ensure that the sample is matched with the sample information, so that the information of the sample after nucleic acid extraction is accurate and the detection result can be traced.
In addition, the nucleic acid extraction device can also realize the quality control function, can detect the performance of nucleic acid extraction equipment, and improves the accuracy of information acquired by the nucleic acid extraction equipment.
The above-mentioned embodiments are only preferred embodiments of the present invention, and not intended to limit the present invention, and various modifications other than the above-mentioned embodiments may be made, and the technical features of the above-mentioned embodiments may be combined with each other, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A nucleic acid extraction device, comprising:
the sample module comprises a first rotating disc, a plurality of clamping grooves are formed in the first rotating disc, the clamping grooves are used for accommodating sample adapters, the sample adapters are used for accommodating sample tubes, and samples are accommodated in the sample tubes;
a reagent module comprising a plurality of solvent bins containing a plurality of reagents;
a pipetting module for extracting and transferring a liquid to be pipetted, the liquid to be pipetted comprising the sample and the reagent;
a reaction module comprising a reaction platform for reacting the sample and the reagent;
the temperature control module is used for controlling the temperature of the reaction platform;
an extraction module for extracting the reacted sample.
2. The nucleic acid extraction device of claim 1, wherein the sample adaptor comprises a main body for receiving the sample tube and an upper cover for covering the sample tube;
the sample module further comprises an uncovering mechanism and a tube-releasing mechanism, the uncovering mechanism is used for opening an upper cover of the sample adapter, and the tube-releasing mechanism is used for guiding the sample adapter to move so that the sample adapter moves out of the clamping groove.
3. The nucleic acid extraction device according to claim 1, wherein the pipetting module comprises a pipetting tip, a pipetting arm and a moving unit, the moving unit is connected with the pipetting arm and is used for driving the pipetting arm to move, a mounting mechanism is arranged on the pipetting arm and is used for mounting the pipetting tip, and the pipetting tip is used for extracting and releasing the liquid to be pipetted.
4. The nucleic acid extraction apparatus according to claim 3, wherein the movement unit includes a first movement unit that moves the pipette arm on a horizontal plane and a second movement unit that moves the pipette arm up and down.
5. The nucleic acid extraction device of claim 1, wherein the reaction module further comprises a displacement assembly for moving the reaction platform to a first predetermined position in a direction close to the pipetting module or to a second predetermined position in a direction away from the pipetting module;
the reaction platform is used for supplying the liquid transferring module to transfer the liquid to be transferred when moving to the first preset position, and the extraction module is positioned above the reaction platform when moving to the second preset position.
6. The nucleic acid extraction device according to claim 1, wherein the extraction module comprises an extraction unit and a lifting mechanism, the extraction unit being connected to the lifting mechanism;
the extraction unit is used for extracting the reacted sample, and the lifting mechanism is used for driving the extraction unit to ascend or descend.
7. The nucleic acid extraction device of claim 1, further comprising a magnetic bead tube and a magnetic attraction system, wherein the extraction module comprises a magnetic rod;
magnetic beads are contained in the magnetic bead tubes, the magnetic attraction system is used for sucking the magnetic beads from the magnetic bead tubes and adding the magnetic beads to the reaction platform, and the magnetic bars are used for sucking the magnetic beads in the reaction platform.
8. The nucleic acid extraction device of claim 1, further comprising a refrigeration module, wherein a stabilizer is disposed within the refrigeration module.
9. The nucleic acid extraction device according to claim 1, further comprising a quality control module containing a quality control standard and a control module comprising a plurality of culture units for containing the reacted sample and the quality control standard.
10. The nucleic acid extraction device of claim 1, further comprising a scanning module for obtaining information of the sample.
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