CN115093954A

CN115093954A - Gene sequencer and using method

Info

Publication number: CN115093954A
Application number: CN202211024468.5A
Authority: CN
Inventors: 张金银; 巩赞博; 巩赞斌; 巩赞华; 黄咏华; 彭劼
Original assignee: Huachenyang Shenzhen Technology Co ltd
Current assignee: Huachenyang Shenzhen Technology Co ltd
Priority date: 2022-08-25
Filing date: 2022-08-25
Publication date: 2022-09-23
Anticipated expiration: 2042-08-25
Also published as: CN115093954B

Abstract

The invention discloses a gene sequencer and a using method thereof, belonging to the technical field of gene sequencing, the gene sequencer comprises an outer shell, wherein a gap is formed in the side wall of the outer shell, a sealing cover is arranged at the gap in a matching manner, a storage mechanism a, a storage mechanism b and a storage mechanism c are arranged in the outer shell, the storage mechanism a is used for storing a kit, a sample and a reagent are stored in the kit, the storage mechanism b is used for storing a suction head, the storage mechanism c is used for storing a sequencing chip, a traction device is also arranged in the outer shell and comprises a traction mechanism and a liquid-transferring mechanism, the traction end of the traction mechanism is detachably connected with the suction head, the traction mechanism is used for drawing the suction head to move between the kit and the sequencing chip, and the liquid-transferring mechanism utilizes the suction head to realize the quantitative dripping of the sample or the reagent in the reagent kit onto the sequencing chip.

Description

Gene sequencer and using method

Technical Field

The invention relates to the technical field of gene sequencing, in particular to a gene sequencer and a using method thereof.

Background

Gene sequencing is a novel gene detection technology, can analyze and determine a gene complete sequence from blood or saliva, and predict the possibility of suffering from various diseases, the core component is a gene sequencing chip, at present, the gene sequencer applied to clinic or research generally needs professional technicians to perform operations such as manual sample loading and the like, the technical requirements are very high, the sample loading quantity cannot be accurately controlled during each sample loading, the sample loading efficiency is extremely low, the sequencing period is long, in addition, a sample reagent is expensive, more reagent quantities can be consumed in a manual sample loading mode, the test cost is invisibly increased, therefore, some gene sequencers for automatic accurate quantitative sample loading appear in the prior art, the manual sample loading is replaced, the accuracy of the sample loading quantity is improved, the sequencing period is reduced, and generally, a reagent tube is drawn to move randomly in a three-dimensional coordinate system, that is, the reagent tube is pulled to move towards or away from the gene sequencing chip, and the reagent in the reagent tube is quantitatively dropped onto the sequencing chip, so as to realize automatic and accurate quantitative sample loading, for example, patent document No. 201710729545.X discloses a gene sequencer, which drives a slide seat to displace along the horizontal direction through a translation driving device, further makes liquid outlets of a plurality of reagent cylinders alternately arranged on the slide seat be alternately positioned above the gene sequencing chip, and drives a sealing piston in the reagent cylinder to move downwards by a set displacement through a liquid supply driving device, so as to realize the purpose that the reagent is quantitatively dropped onto the sequencing chip, however, the automatic and accurate quantitative sample loading mode has the following disadvantages: 1. in the actual sequencing test, based on the base complementary pairing principle, four reagents are provided, and a total of five reagents are provided for washing, wherein on one hand, the reagents are provided

The movement is high-precision movement, and reagent position deviation can occur due to slight deviation to influence the whole sequencing process, so that the precision requirement on a traction structure for pulling the reagent to move is very high, the production cost is high, on the other hand, the reagents are pulled to continuously move and switch between a sequencing chip and a remote sequencing chip, the movement track is a straight line, the displacement is very large, the abrasion accumulation of the traction structure is considerable, and the performance requirement on a material for manufacturing the traction structure is further enhanced; 2. the gene sequencer and the use method thereof are provided on the basis that quantitative sample loading is simply realized, the reagent is simply dripped on the sample, the reagent and the sample are not fully mixed and contacted, the combination between the reagent and the sample is slow, and the sequencing period is long.

Disclosure of Invention

To solve the problems mentioned in the background above, the present invention provides a gene sequencer and a method of use.

In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows.

A gene sequencer comprises an outer shell, wherein a gap is formed in the side wall of the outer shell, a sealing cover is installed at the gap in a matching mode, a storage mechanism a, a storage mechanism b and a storage mechanism c are arranged in the outer shell, the storage mechanism a is used for storing a kit, a sample and a reagent are stored in the kit, the storage mechanism b is used for storing a suction head, and the storage mechanism c is used for storing a sequencing chip;

still be provided with draw gear in the shell, draw gear includes drive mechanism and moves liquid mechanism, and detachable the being connected between drive mechanism's the end of pulling and the suction head, drive mechanism are used for pulling the suction head and take place to remove between kit and sequencing chip, and move liquid mechanism and utilize the suction head to realize sample in the kit or reagent ration drippage to sequencing chip.

Further, the storage mechanism a comprises a linear module a and an upright post, the linear module a is used for driving the upright post to move, the moving direction of the linear module a is perpendicular to the side wall of the outer shell body provided with the notch, a tray which is horizontally arranged is installed on the upright post, and a baffle is arranged at one end, far away from the notch, of the tray;

the reagent box passes through to constitute detachable sliding guide cooperation between guide and the tray, and the up end of reagent box evenly spaced is provided with multiunit test tube hole, is provided with the test tube in the test tube hole, is equipped with sample or reagent in the test tube.

Further, deposit mechanism c including installing the grudging post in the shell body, the grudging post is close to the breach, installs the saddle that is the level and arranges on the grudging post, and the up end of saddle is provided with mounting groove and runs through the both sides face of saddle along stand moving direction, and slidable mounting has the pole that moves back in the mounting groove, is provided with on the grudging post to be used for ordering about to move back the pole and takes place gliding sharp module b.

Furthermore, a storage mechanism b is positioned between the storage mechanism a and the storage mechanism c, the storage mechanism b comprises an installation frame fixedly arranged at the notch, the installation frame is in a rectangular ring shape, a buckling frame is detachably arranged in the installation frame, and a storage part is arranged on the buckling frame facing to the side surface of the inner cavity of the outer shell;

the storage component comprises a supporting rod and a limiting plate which are vertically arranged on the buckling frame, the side surface of the supporting rod, which is far away from the buckling frame, is provided with an installation opening which penetrates through the height of the supporting rod, the side wall of the installation opening, which faces the buckling frame, is an arc-shaped hole bottom, the upper end surface of the supporting rod is provided with a supporting groove b which is coaxially arranged with the hole bottom of the installation opening, the two hole walls of the installation opening are respectively provided with a supporting groove a which is in an arc groove shape, the two groups of supporting grooves a are coaxially arranged, and the axis line of the two groups of supporting grooves a is parallel to the axis line of the supporting groove b;

the limiting plate is positioned above the supporting rod, the supporting groove a is positioned on one side of the limiting plate, which is far away from the buckling frame, an avoidance opening which penetrates through the height of the limiting plate is arranged on the side surface of the limiting plate, which is far away from the buckling frame, the side wall of the avoidance opening, which faces the buckling frame, is an arc-shaped hole bottom, and the hole bottom of the avoidance opening and the hole bottom of the mounting opening are coaxially arranged;

the support groove a and the support groove b are equal in diameter, the hole bottom of the avoidance opening and the hole bottom of the installation opening are equal in diameter, the hole bottom diameter of the installation opening is smaller than that of the support groove b, an external step is arranged at the upper opening end of the suction head, the diameter of the suction head is smaller than that of the hole bottom of the installation opening, and the suction head is placed at the support groove a through the matching of the external step and the support groove a in an initial state.

Further, drive mechanism includes sharp module c, sharp module d and sharp module e, and the output of sharp module e is provided with mounting bracket and support bracket, and sharp module c, sharp module d and sharp module e, the three cooperation are used for ordering about mounting bracket and support bracket and take place the displacement in the three-dimensional coordinate system.

Furthermore, a spindle which is vertically arranged is arranged on the mounting frame, a first motor is mounted on the mounting frame, a worm gear and a worm are arranged between the output end of the first motor and the spindle, the worm gear and the worm comprise a worm which is arranged at the output end of the first motor and a worm wheel which is coaxially sleeved outside the spindle through a bearing, a cantilever is arranged outside a worm wheel shaft of the worm wheel along the radial direction, and a clamping joint is vertically arranged downwards at the bottom of a suspension end of the cantilever;

the sample position, the sequencing chip position and the suction head position in the kit, and the diameter of the circumscribed circle of the three parts is equal to the distance between the axis line of the clamping joint and the axis line of the main shaft.

Further, the liquid transfer mechanism comprises a cylinder shell vertically arranged on the support bracket, and the cylinder shell is divided into three sections along the vertical direction and sequentially comprises a bottom cylinder shell, a middle cylinder shell and an upper cylinder shell from bottom to top;

the upper end and the lower end of the upper cylinder shell are opened, the upper opening end is provided with a motor II through a motor frame, the output end of the motor II is provided with a screw rod I through a coupler, and the screw rod is coaxially positioned in the upper cylinder shell;

the upper end of the middle cylinder shell is open, the lower end of the middle cylinder shell is closed, the upper open end of the middle cylinder shell is coaxially fixed with the lower open end of the upper cylinder shell, a piston is arranged in the middle cylinder shell in a sliding mode, a piston rod is coaxially arranged on the upper end face of the piston, the piston rod is hollow, the top end of the piston rod extends into the upper cylinder shell and is in threaded connection with the screw rod, and the outer wall of the piston rod is in sliding guide fit with the inner wall of the upper cylinder shell in the vertical direction through a sliding piece;

the lower closed end of the middle cylinder shell coaxially extends to form a connecting sleeve, the upper end opening and the lower end of the bottom cylinder shell are closed and coaxially sleeved outside the connecting sleeve, the lower closed end of the middle cylinder shell is provided with a connecting hole used for communicating between the middle cylinder shell and the bottom cylinder shell, the closed end of the bottom cylinder shell coaxially extends to form a connecting nozzle, and the top end of the connecting nozzle extends into the bottom cylinder shell and is provided with a rubber cap, and the bottom end of the connecting nozzle is communicated with the clamping joint through the connecting pipe.

Furthermore, an imaging mechanism is also arranged in the outer shell, and a display screen is also arranged on the outer shell;

the imaging mechanism comprises a linear module f, a linear module g and a fluorescence scanner, the linear module f and the linear module g are matched for driving the fluorescence scanner to be positioned right above the sequencing chip or far away from the sequencing chip, and the fluorescence scanner is used for scanning the sequencing result on the sequencing chip, taking pictures and transmitting the pictures to the display screen;

a heating element is also disposed within the outer shell.

A use method of a gene sequencer is provided, wherein four reagents are arranged in a kit, and a sample and the four reagents in the kit sequentially comprise the following steps along the direction that a storage mechanism a points to a storage mechanism c: the method comprises the following steps of:

the method comprises the following steps: the position of the sample, the position of the sequencing chip and the position of the suction head, and the circumcircle center of the three parts is named as a point a;

the linear module d and the linear module e are matched to drive the spindle to move, so that the point a is positioned on the axis line of the spindle;

the first motor operates to drive the cantilever to rotate, so that the clamping joint is positioned right above the suction head;

the operation of the linear module c drives the clamping head to move downwards so that the suction head is clamped on the clamping head, and the operation of the linear module c drives the clamping head to move upwards so that the suction head leaves the storage component;

step two: the first motor operates to drive the cantilever to rotate, so that the suction head is positioned right above the sample;

the operation of the linear module c drives the suction head to move downwards to be inserted into the test tube with the sample, the liquid-moving mechanism drives the suction head to suck the quantitative sample, and the operation of the linear module c drives the suction head to move upwards to leave the test tube;

the first motor operates to drive the cantilever to rotate, so that the suction head is positioned right above the sequencing chip, and the pipetting mechanism operates to enable the sample in the suction head to drip downwards onto the sequencing chip;

step three: the central line of a connecting line between the position of the reagent a and the position of the sequencing chip is a central line a, a point b exists on the central line a, the distance between the point b and the reagent a is equal to the distance between the point b and the sequencing chip, and the distance is equal to the distance between the axial line of the clamping joint and the axial line of the main shaft;

the linear module d and the linear module e are matched to drive the spindle to move, so that the point b is positioned on the axis line of the spindle;

the first motor operates to drive the cantilever to rotate, so that the sucker is positioned right above the reagent a;

the operation of the linear module c drives the suction head to move downwards to be inserted into a test tube filled with a reagent a, the operation of the liquid-transfering mechanism enables the suction head to suck a quantitative reagent a, and the operation of the linear module c drives the suction head to move upwards to leave the test tube;

the first motor operates to drive the cantilever to rotate, so that the suction head is positioned right above the sequencing chip, and the liquid-transferring mechanism operates to enable the reagent a in the suction head to drop downwards onto the sequencing chip;

step four: the central line of a connecting line between the position of the reagent b and the position of the sequencing chip is a central line b, a point c exists on the central line b, the distance between the point c and the reagent b is equal to the distance between the point c and the sequencing chip, and the distance is equal to the distance between the axial line of the clamping joint and the axial line of the main shaft;

repeating the third step to enable the point c to be positioned on the axis line of the main shaft and realize the quantitative dripping of the reagent b on the sequencing chip;

step five: the central line of a connecting line between the position of the reagent c and the position of the sequencing chip is a central line c, a point d exists on the central line c, the distance between the point d and the reagent c is equal to the distance between the point d and the sequencing chip, and the distance is equal to the distance between the axis line of the clamping joint and the axis line of the main shaft;

repeating the third step to enable the point d to be positioned on the axial core line of the main shaft and realize the quantitative dripping of the reagent c on the sequencing chip;

step six: the central line of a connecting line between the position of the reagent d and the position of the sequencing chip is a central line d, a point e exists on the central line d, the distance between the point e and the reagent d is equal to the distance between the point e and the sequencing chip, and the distance is equal to the distance between the axis line of the clamping joint and the axis line of the main shaft;

and repeating the third step to enable the point e to be positioned on the axis line of the main shaft, and realizing the quantitative dripping of the reagent d onto the sequencing chip.

Further, the pipetting mechanisms in the second step, the third step, the fourth step, the fifth step and the sixth step operate:

s1: after the suction head is inserted into the test tube, the second motor operates to drive the piston to vertically move downwards for a preset distance a, so that the volume of the area between the piston and the closed end of the middle cylinder shell is reduced, the pressure is increased, the pressure in the bottom cylinder shell is changed along with the change of the pressure, and the outer surface of the rubber cap is squeezed and shrunk by the pressure;

the second motor operates to drive the piston to move upwards for a preset distance a, so that the volume of the area between the piston and the closed end of the middle cylinder shell is increased and recovered, the pressure is gradually reduced and recovered, the rubber cap is gradually expanded and recovered from being deflated, and finally the sample or the reagent in the test tube is quantitatively sucked into the suction head;

s2: when the suction head is positioned right above the sequencing chip, the second motor operates to drive the piston to vertically move downwards by a preset distance a, so that a sample or a reagent in the suction head drops downwards onto the sequencing chip;

the motor II operates to continuously drive the piston to vertically move downwards for a preset distance b, in the process, the rubber cap is continuously compressed, air in the suction head is discharged downwards through the connecting pipe to form a jet of air flow, the air flow blows the inner wall of the suction head, residual samples or residual reagents are blown by the air flow to drip downwards onto the sequencing chip, the air flow blows the samples or the reagents on the sequencing chip, the samples are evenly spread on the sequencing chip, and the samples and the reagents are evenly mixed;

after the cantilever rotates to enable the suction head to be far away from the sequencing chip, the second motor operates to drive the piston to vertically move upwards for a preset distance b and a preset distance a to reset.

Compared with the prior art, the invention has the beneficial effects that:

one, in this scheme, the sample or reagent to the in-process of the ration drippage on the sequencing chip, straight line module d and the cooperation of straight line module e, only need pull the main shaft and in point a, point b, point c, point d, point e, take place between these five points the displacement can, other displacements are replaced by cantilever rotation, and its meaning lies in: 1. compared with the prior art, the distance between the five points is shorter, so that the running tracks of the linear module d and the linear module e are shorter, the abrasion generated during running is less, and the service life of the linear module is greatly prolonged; 2. the suction head is driven to switch between the sample or the reagent and the sequencing chip through the rotation of the cantilever, so that the time spent on sample loading can be reduced, if not, the suction head is switched between the sample or the reagent and the sequencing chip through the matching of the linear module d and the linear module e, and because a pause exists between the operation switching of the linear module d and the linear module e and the sum of the driving track of the linear module d and the driving track of the linear module e is greater than the action track of the cantilever, the sample loading period is prolonged; 3. the cantilever rotation action replaces two actions of driving the suction head to move by the linear module d and driving the suction head to move by the linear module e, so that the action error is smaller, and the sample loading is more accurate.

In the scheme, in the dripping process of the sample or the reagent on the sequencing chip: after sample or reagent in the suction head drop to sequencing chip on downwards, motor two continues to drive the piston and moves down vertically and predetermine distance b, and this in-process, the rubber cap continues to be shriveled, makes the air in the suction head discharge downwards through the connecting pipe and forms one air current, and its meaning lies in: on one hand, the air flow can blow the inner wall of the suction head, if a sample or a reagent remains on the inner wall of the suction head, the residual sample or the reagent can be dripped downwards by the air flow so as to ensure the accuracy of the sample loading amount, on the other hand, the sample dripped downwards from the suction head to the sequencing chip is generally in an upward-bulging water drop shape, the air flow can blow the sample so that the upward-bulging water drop shape is changed into a flat shape on the sequencing chip, and then after the reagent falls downwards, the air flow also blows the reagent and the sample, so that the contact area between the sample and the reagent can be increased, the sample and the reagent are uniformly mixed, the combination between the sample and the reagent is quicker, and the sequencing period is shortened.

Drawings

FIG. 1 is a schematic view of the present invention with the closure opened;

FIG. 2 is a schematic view of the present invention after the closure is closed;

FIG. 3 is a schematic view of the internal structure of the present invention; FIG. 4 is a schematic structural view of a storage mechanism a; FIG. 5 is a schematic view of the storage mechanism b; FIG. 6 is a schematic structural view of a storage mechanism c;

FIG. 7 is a schematic view of the drawing apparatus, the imaging mechanism, and the heating element;

FIG. 8 is a schematic view of the structure of the imaging mechanism;

FIG. 9 is a partial schematic view of the draft gear;

FIG. 10 is a partial schematic view of a first embodiment of the traction mechanism;

FIG. 11 is a second partial schematic view of the traction mechanism;

FIG. 12 is an exploded view of the pipetting mechanism;

FIG. 13 is a cross-sectional view of the base cartridge shell;

FIG. 14 is a cross-sectional view of the mid-cylinder shell;

fig. 15 is a cross-sectional view of the upper cartridge housing. The reference numbers in the drawings are:

100. an outer housing; 101. sealing the cover; 102. a display screen;

200. a storage mechanism a; 201. a linear module a; 202. a column; 203. a tray;

204. a baffle plate; 205. a kit; 206. a chute; 207. a sliding projection;

300. a storage mechanism b; 301. installing a frame; 302. buckling a frame; 303. a support bar;

304. a limiting plate; 305. a support groove a; 306. a support groove b; 307. avoiding the mouth; 308. a suction head;

400. a storage mechanism c; 401. erecting a frame; 402. a saddle; 403. withdrawing the rod; 404. a linear module b; 405. a connecting frame; 406. sequencing the chip;

500. a traction device; 501. a linear module c; 502. a linear module d; 503. a linear module e; 504. a mounting frame; 505. a main shaft; 506. a first motor; 507. a worm gear; 508. a cantilever; 509. clamping a connector; 510. a support bracket; 511. a connecting pipe;

512. a base shell; 513. a middle cylinder shell; 514. an upper cartridge shell; 515. a connecting nozzle; 516. a rubber cap; 517. a pressure sensor; 518. connecting sleeves; 519. connecting holes; 520. a piston; 521. a piston rod; 522. a first screw rod; 523. a guide groove; 524. a second motor;

600. an imaging mechanism; 601. a linear module f; 602. a linear module g; 603. a fixed mount; 604. a main rod; 605. separating rods; 606. a fluorescence scanner;

700. a heating element.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.

As shown in fig. 1-15, a gene sequencer includes an outer casing 100, a gap is opened on a side wall of the outer casing 100, and a cover 101 is fittingly installed at the gap.

Three groups of storage mechanisms are arranged in the outer shell 100: the storage mechanism a200, the storage mechanism b300 and the storage mechanism c400 are used, wherein the storage mechanism a200 is used for storing the reagent box 205, samples and reagents are stored in the reagent box 205, the storage mechanism b300 is used for storing the suction head 308, the storage mechanism c400 is used for storing the sequencing chip 406, the sealing cover 101 is opened, the reagent box 205, the suction head 308 and the sequencing chip 406 can be respectively placed in the three groups of storage mechanisms, and after the reagent box 205, the suction head 308 and the sequencing chip 406 are placed, the sealing cover 101 is closed, and gene sequencing is carried out.

Storage mechanism a 200:

as shown in fig. 3 and 4, the storage mechanism a200 includes a linear module a201, the linear module a201 includes a guide bracket a installed in the outer casing 100, a lead screw a and a motor a are installed on the guide bracket a, the motor a is in power connection with the lead screw a, and both an axial direction of the lead screw a and a guiding direction of the guide bracket a are perpendicular to a side wall of the outer casing 100 where the notch is provided.

And a sliding seat a is arranged on the external thread of the screw rod a, the sliding seat a and the guide support a form sliding guide fit, and when the motor a operates to drive the screw rod a to rotate, the sliding seat a displaces along the guide direction of the guide support a.

The upper end face of the sliding seat a is provided with a vertical column 202 which is vertically arranged, a tray 203 which is horizontally arranged is mounted on the vertical column 202, and a baffle 204 is arranged at one end of the tray 203 which is far away from the gap along the axial direction of the screw rod a.

The reagent box 205 is detachably matched with the tray 203 in a sliding and guiding manner through a guide piece, and specifically, the guide piece comprises a sliding protrusion 207 arranged on the side surface of the reagent box 205 and a sliding groove 206 arranged on the side surface of the tray 203.

When the cover 101 is opened, the motor a operates to drive the sliding seat a to move, the sliding seat a moves to drive the upright column 202 to move together with the tray 203, so that part of the tray 203 extends out of the gap, the sliding protrusion 207 is inserted into the sliding groove 206 after facing the sliding groove 206, the reagent box 205 is slidably mounted on the tray 203, when the reagent box 205 is in contact with the baffle 204, the reagent box 205 is mounted, the motor a moves reversely, so that the tray 203 retracts into the outer shell 100, in the process, the baffle 204 plays a positioning role, otherwise, the motor a operates to drive the tray 203 to extend out of the gap, and a worker can take the reagent box 205 out of the tray 203.

A plurality of groups of test tube holes are uniformly arranged on the upper end surface of the kit 205 at intervals, test tubes are arranged in the test tube holes, and samples or reagents are filled in the test tubes.

Storage mechanism c 400:

as shown in fig. 3 and fig. 6, the storage mechanism c400 includes a vertical frame 401 installed in the outer casing 100, the vertical frame 401 is close to the gap, a horizontally arranged tray 402 is installed on the vertical frame 401, a mounting groove is provided on the upper end surface of the tray 402 and penetrates through two side surfaces of the tray 402 along the axial direction of the screw rod a, a withdrawing rod 403 is slidably installed in the mounting groove, a linear module b404 for driving the withdrawing rod 403 to slide is provided on the vertical frame 401, specifically, the linear module b404 includes a motor b and a screw rod b installed on the vertical frame 401, the motor b and the screw rod b are in power connection and parallel to the screw rod a, a sliding seat b is installed on the external thread of the screw rod b, and the sliding seat b and the vertical frame 401 form a sliding fit with a guiding direction parallel to the axial direction of the screw rod b, the slide b is connected with the retreating rod 403 through a connecting frame 405.

The sealing cover 101 is opened, the placing groove is close to the notch, preferably, one side of the installing groove facing outdoors is flush with the notch, so that the sequencing chip can be directly inserted into the installing groove, the withdrawing rod 403 is positioned at one end of the installing groove deviating from the notch in an initial state, when the sequencing chip is contacted with the withdrawing rod 403, the installation of the sequencing chip is completed, and the withdrawing rod 403 plays a role in positioning;

when motor b operation orders about lead screw b and rotates, slide b takes place the displacement along lead screw b's guiding direction, and slide b removes to take back pole 403 through link 405 and removes together, and this moment, it does the removal that is close to the breach to back pole 403, moves back pole 403 and supports to push sequencing chip and remove together, makes sequencing chip part stretch out the mounting groove, and at this moment, the staff can directly take off sequencing chip, and it plays the supplementary effect of taking sequencing chip to back pole 403.

Storage mechanism b 300:

as shown in fig. 3, the storage mechanism b300 is located between the storage mechanism a200 and the storage mechanism c400, and in order to avoid obstructing the movement track of the traction device 500, the column 202 is located on the side of the tray 203 facing away from the storage mechanism b300, and the stand 401 is located on the side of the pallet 402 facing away from the storage mechanism b 300.

As shown in fig. 3 and 5, the storage mechanism b300 includes a mounting frame 301 fixedly disposed at the notch, the mounting frame 301 is in a rectangular ring shape, the ring surface of the mounting frame is parallel to the opening surface of the notch, a buckle frame 302 is detachably mounted in the mounting frame 301, the buckle frame 302 is in a rectangular plate shape, a handle is disposed on one side of the buckle frame which deviates from the inner cavity of the outer shell 100, and the detachable manner specifically includes: the buckle frame 302 is made of elastic materials, such as plastics, and has certain elastic deformation capacity, and the outer wall of the buckle frame 302 is in interference fit with the inner wall of the installation frame 301, so that the buckle frame 302 can be directly plugged into the installation frame 301 and slightly pressed, the buckle frame 302 can be installed in the installation frame 301, the buckle frame 302 is pulled outwards, the buckle frame 302 can be taken away from the installation frame 301, the detachable mode is common in real life, and the detachable mode can be realized for the prior art without repeated description.

As shown in fig. 5, the side of the buckle frame 302 facing the inner cavity of the outer housing 100 is provided with a storage part for storing the suction head 308 or assisting in detaching the suction head 308 from the towing apparatus 500.

Specifically, the storage component comprises a support rod 303 and a limiting plate 304 which are vertically arranged on the buckle frame 302.

The side of bracing piece 303 deviating from detain frame 302 is provided with the installing port that runs through its height, and the lateral wall of installing port orientation detain frame 302 is provided with the support groove b306 of arranging with the installing port hole bottom for the hole bottom that is arc shape and the up end of bracing piece 303, and two hole walls of installing port all are provided with the support groove a305 that is arc groove shape, and two sets of support groove a305 are arranged coaxially and the axle heart yearn is on a parallel with the axle heart yearn of support groove b 306.

The limiting plate 304 is located above the supporting rod 303, the length of the limiting plate 304 is smaller than that of the supporting rod 303, the supporting groove a305 is located on one side of the limiting plate 304, which deviates from the buckling frame 302, an avoiding opening 307 running through the height of the limiting plate 304 is formed in the side face of the limiting plate 304, which deviates from the buckling frame 302, and the side wall of the avoiding opening 307, which faces the buckling frame 302, is coaxially arranged with the hole bottom of the mounting opening and the hole bottom of the avoiding opening 307.

The support groove a305 and the support groove b306 have the same diameter, the hole bottom of the relief opening 307 and the hole bottom of the mounting opening have the same diameter, and the hole bottom diameter of the mounting opening is smaller than the diameter of the support groove b 306.

An external step is arranged at the upper opening end of the suction head 308, the diameter of the suction head 308 is smaller than the diameter of the bottom of a hole of an installation opening, the diameter of the external step is slightly smaller than the diameter of the support groove a305, in an initial state, the suction head 308 is placed at the support groove a305 through the matching of the external step and the support groove a305, after the whole gene sequencing is finished, the traction device 500 pulls the suction head 308 to move to the support groove b306, the suction head 308 is placed at the support groove b306 through the matching of the support groove b306 and the external step, then, the traction device 500 moves upwards vertically, under the blocking of the matching of the limiting plate 304 and the external step, the suction head 308 cannot move upwards along with the traction device 500, so the installation is disconnected when the traction device 500 and the suction head 308 are disassembled, at the moment, the suction head 308 is placed at the support groove b306, is a used waste tip.

In addition, in a preferred embodiment, as shown in fig. 3-6, the trays 203, the pallets 402 and the suckers 308 are all arranged in a plurality of groups in an array, which means that gene sequencing of a plurality of samples can be performed at one time and the sequencing of a plurality of genes is not interfered with each other, for example, in the drawings of the present specification, three groups of trays 203 and pallets 402 are arranged in an array along the vertical direction, so that three groups of gene sequencing can be performed simultaneously.

As shown in fig. 3 and fig. 7-15, a traction device 500 is further disposed in the outer casing 100, the traction device 500 includes a traction mechanism and a liquid-transferring mechanism, and a traction end of the traction mechanism is detachably connected with the suction head 308, specifically, the detachable manner is a snap-fit connection.

The drawing mechanism is used for drawing the sucker 308 to move between the reagent box 205 and the sequencing chip 406, and the pipetting mechanism realizes the quantitative dripping of the sample or the reagent in the reagent box 205 onto the sequencing chip 406 by using the sucker 308.

As shown in fig. 7 and 9 to 11, the traction mechanism includes a linear module c501, a linear module d502, and a linear module e 503.

Wherein, straight line module c501 is including installing the body frame body in shell body 100 and vertically installing the lead screw c on the body frame body, and lead screw c is connected with motor c power, and slide c is installed to lead screw c's outside screw thread, and slide c constitutes the vertical sliding fit of direction of guide with the body frame body simultaneously, and when motor c moved and orders about lead screw c and rotates, slide c took place the displacement along vertical direction.

The straight line module d502 includes the guide bracket d of being connected with slide c, install lead screw d and motor d on the guide bracket d, both power connection, the axial of lead screw d and the direction of guide bracket d all are perpendicular to the axial of lead screw a and the axial of perpendicular to lead screw c, slide d is installed to the outside screw thread of lead screw d, slide d constitutes sliding fit with guide bracket d simultaneously, when slide c takes place to remove along vertical direction, take straight line module d502 to remove together, in addition, when motor d moves and orders about lead screw d and rotates, slide d takes place the displacement along the direction of guide bracket d.

The linear module e503 comprises a guide support e connected with a slide seat d, a screw rod e and a motor e are mounted on the guide support e and are in power connection, the axial direction of the screw rod e and the guide direction of the guide support e are both parallel to the axial direction of the screw rod a, a slide seat e is mounted on the external thread of the screw rod e, the slide seat e and the guide support e form sliding fit, when the slide seat d moves, the linear module e503 is driven to move together, and in addition, when the motor e runs to drive the screw rod e to rotate, the slide seat e moves along the guide direction of the guide support e.

As shown in fig. 10, the slide e is further provided with an installation frame 504 and a support bracket 510, when the slide e moves, the installation frame 504 and the support bracket 510 are driven to move together, that is, the linear module c501, the linear module d502 and the linear module e503 are matched, and the installation frame 504 and the support bracket 510 can be pulled to move in a three-dimensional coordinate system.

As shown in fig. 10 and 11, a spindle 505 arranged vertically is disposed on the mounting bracket 504, a first motor 506 is further mounted on the mounting bracket 504, a worm gear 507 is disposed between an output end of the first motor 506 and the spindle 505, specifically, the worm gear 507 includes a worm disposed at an output end of the first motor 506 and a worm wheel coaxially sleeved outside the spindle 505, the worm gear is engaged with the worm wheel, the worm wheel and the spindle 505 form a rotational connection, preferably, the worm wheel is assembled with the spindle 505 through a bearing, a cantilever 508 is disposed outside a worm wheel shaft of the worm wheel along a radial direction, and a clamping joint 509 is disposed at a bottom of a suspension end of the cantilever 508 downward vertically.

In addition, as shown in fig. 3, the sample position in the kit 205, the position of the sequencing chip 406, and the position of the pipette tip 308 are distributed in an obtuse triangle, so that the center of the circumscribed circle is close to the inner wall of the outer shell 100 away from the gap.

The operation of the first motor 506 drives the cantilever 508 to rotate around the axis line of the main shaft 505 through the worm gear 507, and the worm gear 507 has self-locking performance, so that the cantilever 508 does not rotate when the first motor 506 does not operate, and in addition, the cantilever 508 is provided with a plurality of groups corresponding to the tray 203 and the pallet 402, for example, in the drawings of the specification of the scheme, three groups of the tray 203 and the pallet 402 are provided, so that three groups of the cantilever 508 are also provided correspondingly. The working process of the traction mechanism is specifically represented as follows:

the sample and the four reagents in the reagent kit 205 are sequentially arranged along the direction from the storage mechanism a200 to the storage mechanism c 400: a sample, a reagent a, a reagent b, a reagent c, and a reagent d; firstly, the method comprises the following steps: the position of the sample, the position of the sequencing chip 406 and the position of the suction head 308, and the circumscribed circle center of the three is named as a point a;

the straight line module d502 and the straight line module e503 are matched with each other to pull the main shaft 505 to move, so that the point a is positioned on the axis line of the main shaft 505;

the operation of the first motor 506 drives the cantilever 508 to rotate, so that the card joint 509 is positioned right above the suction head 308;

the linear module c501 operates to drive the clamping head 509 to move vertically downwards, an interference fit is formed between the outer wall of the clamping head 509 and the inner wall of the upper opening end of the suction head 308, the suction head 308 is made of plastic and has certain elastic deformation, so that the clamping head 509 moves downwards to be directly inserted into the suction head 308, the suction head 308 is clamped on the clamping head 509, and the linear module c501 operates to drive the clamping head 509 to move vertically upwards to enable the suction head 308 to leave the storage component;

II, secondly: the first motor 506 operates to drive the cantilever 508 to rotate, so that the sucker 308 is positioned right above the sample;

the operation of the linear module c501 drives the suction head 308 to move vertically downwards, so that the suction head 308 is inserted into a test tube filled with a sample, the liquid-moving mechanism operates, so that the sample is quantitatively sucked into the suction head 308, and the operation of the linear module c501 drives the suction head 308 to move vertically upwards and leave the test tube;

the operation of the first motor 506 drives the cantilever 508 to rotate, so that the suction head 308 is positioned right above the sequencing chip 406 and the interval between the bottom of the suction head 308 and the sequencing chip 406 is small;

the pipetting mechanism operates to drip the sample in the pipette tip 308 down into the sequencing chip 406;

thirdly, the steps of: the central line of the connecting line between the position of the reagent a and the position of the sequencing chip 406 is a central line a, a point b is arranged on the central line a, the distance between the point b and the reagent a is equal to the distance between the point b and the sequencing chip 406, and the distance is equal to the distance between the axial line of the clamping joint 509 and the axial line of the main shaft 505;

the straight line module d502 and the straight line module e503 are matched with each other to pull the main shaft 505 to move, so that the point b is positioned on the axial centerline of the main shaft 505;

the first motor 506 operates to drive the cantilever 508 to rotate, so that the sucker 308 is positioned right above the reagent a;

the operation of the linear module c501 drives the suction head 308 to move vertically downwards, so that the suction head 308 is inserted into a test tube filled with a reagent a, the liquid-moving mechanism operates, so that the reagent a is quantitatively sucked into the suction head 308, and the operation of the linear module c501 drives the suction head 308 to move vertically upwards and leave the test tube;

the operation of the first motor 506 drives the cantilever 508 to rotate, so that the suction head 308 is positioned right above the sequencing chip 406;

the pipetting mechanism operates to make the reagent a in the sucker 308 drop downwards into the sequencing chip 406;

fourthly, the method comprises the following steps: the central line of the connecting line between the position of the reagent b and the position of the sequencing chip 406 is a central line b, a point c is arranged on the central line b, the distance between the point c and the reagent b is equal to the distance between the point c and the sequencing chip 406, and the distance is equal to the distance between the axial line of the clamping joint 509 and the axial line of the main shaft 505;

repeating step three to realize quantitative dripping of the reagent b on the sequencing chip 406;

fifthly: the central line of the connecting line between the position of the reagent c and the position of the sequencing chip 406 is a central line c, a point d is arranged on the central line c, the distance between the point d and the reagent c is equal to the distance between the point d and the sequencing chip 406, and the distance is equal to the distance between the axial line of the clamping joint 509 and the axial line of the spindle 505;

repeating step three to realize quantitative dripping of the reagent c on the sequencing chip 406;

sixthly: the central line of the connecting line between the position of the reagent d and the position of the sequencing chip 406 is a central line d, a point e is arranged on the central line d, the distance between the point e and the reagent d is equal to the distance between the point e and the sequencing chip 406, and the distance is equal to the distance between the axial line of the clamping joint 509 and the axial line of the spindle 505;

three iterations are then performed to achieve quantitative dripping of reagent d onto sequencing chip 406.

According to the sequence of the third, fourth, fifth and sixth, reagents or samples are correspondingly stored in the test tubes in the kit 205 one by one in advance;

in the quantitative dripping process of the sample or the reagent on the sequencing chip 406, the linear module d502 is matched with the linear module e503, only the main shaft 505 needs to be pulled to displace between the point a, the point b, the point c, the point d and the point e, and the other displacements are replaced by the rotation of the cantilever 508, so that the significance is as follows: 1. compared with the prior art, the distance between the five points is shorter, so that the running tracks of the straight line module d502 and the straight line module e503 are shorter, the abrasion generated by the running is less, and the service life of the linear module is greatly prolonged; 2. the cantilever 508 rotates to drive the sucker 308 to switch between the sample or the reagent and the sequencing chip 406, so that the time spent on sample loading can be reduced, if not, the sucker 308 is switched between the sample or the reagent and the sequencing chip 406 through the matching of the linear module d502 and the linear module e503, and the sample loading period is prolonged because a pause exists between the operation switching of the linear module d502 and the linear module e503 and the sum of the driving track of the linear module d502 and the driving track of the linear module e503 is greater than the action track of the cantilever 508; 3. the action of rotating the cantilever 508 replaces the actions of driving the suction head 308 to move by the linear module d502 and driving the suction head 308 to move by the linear module e503, so that the action error is smaller and the sample loading is more accurate.

As shown in fig. 10-15, the pipetting mechanism is mounted on the support bracket 510, and the pipetting mechanism includes a barrel casing vertically disposed on the support bracket 510, which is divided into three sections along the vertical direction and sequentially includes a bottom barrel casing 512, a middle barrel casing 513 and an upper barrel casing 514 from bottom to top.

As shown in fig. 15, the upper end and the lower end of the upper cylindrical shell 514 are open, the upper open end is provided with a second motor 524 through a motor frame, the output end of the second motor 524 is provided with a first screw rod 522 through a coupling, and the first screw rod 522 is coaxially located in the upper cylindrical shell 514.

As shown in fig. 14, the upper end of the middle cylindrical shell 513 is open, the lower end of the middle cylindrical shell is closed, the upper open end of the middle cylindrical shell is coaxially fixed with the lower open end of the upper cylindrical shell 514, a piston 520 is slidably installed inside the middle cylindrical shell, a piston rod 521 is coaxially arranged on the upper end surface of the piston 520, the piston rod 521 is hollow inside, the top end of the piston rod 521 extends into the upper cylindrical shell 514 and is in threaded connection with a first screw rod 522, the outer wall of the piston rod 521 is further in sliding guiding fit with the inner wall of the upper cylindrical shell 514 through a sliding part, specifically, the sliding part comprises a guide groove 523 arranged on the upper cylindrical shell 514 and a guide protrusion arranged on the piston rod 521, and the guide protrusion and the guide groove 523 form sliding guiding fit in the vertical direction; when the second motor 524 operates to rotate the first screw 522, the volume of the area between the piston 520 and the closed end of the middle drum 513 is changed by driving the piston rod 521 to move the piston 520 upwards or downwards.

As shown in fig. 13 and 14, a connection sleeve 518 coaxially extends from the lower closed end of the middle drum shell 513, the upper end of the bottom drum shell 512 is open, the lower end of the bottom drum shell 512 is closed, and the bottom drum shell 512 is coaxially sleeved outside the connection sleeve 518, and a connection hole 519 for communicating the middle drum shell 513 and the bottom drum shell 512 is further formed in the lower closed end of the middle drum shell 513.

The closed end of the bottom cylinder shell 512 coaxially extends to form a connecting nozzle 515, the top end of the connecting nozzle 515 extends into the bottom cylinder shell 512 and is provided with a rubber cap 516, and the bottom end of the connecting nozzle is communicated with the clamping joint 509 through a connecting pipe 511.

The working process of the liquid transferring mechanism is as follows:

when the suction head 308 is inserted into the test tube, the second motor 524 operates to drive the piston 520 to move vertically downwards for a preset distance a and then move vertically upwards for the preset distance a, the former can reduce the volume of the area between the piston 520 and the closed end of the middle cylinder shell 513, increase the pressure, further increase the pressure in the bottom cylinder shell 512, the outer surface of the rubber cap 516 is squeezed and deflated by the pressure, the latter can restore the enlarged volume of the area between the piston 520 and the closed end of the middle cylinder shell 513, the pressure also gradually reduces and restores, the rubber cap 516 also gradually bulges and restores from being deflated, a process similar to the pipette of a burette is simulated, the change of the rubber cap 516 is transmitted to the suction head 308 through the connecting pipe 511, further, the sample or reagent in the test tube is sucked into the suction head 308, and the downward movement distance of the piston 520 is the preset distance a, is set in advance according to the sample loading quantity, thereby realizing the purpose of quantitative liquid suction of the suction head 308;

when the suction head 308 is located right above the sequencing chip 406, the second motor 524 operates to drive the piston 520 to move vertically downwards by a preset distance a, so that the sample or reagent in the suction head 308 drops downwards onto the sequencing chip 406, and then the second motor 524 operates to drive the piston 520 to move vertically downwards by a preset distance b, in the process, the rubber cap 516 continues to be deflated, and the air in the suction head 308 is discharged downwards through the connecting pipe 511 to form an air flow, which has the following significance: on one hand, the air flow can blow the inner wall of the suction head 308, if a sample or a reagent remains on the inner wall of the suction head 308, the remaining sample or reagent is blown by the air flow to drop downwards to ensure the accuracy of the sample loading amount, on the other hand, the sample dropping downwards from the suction head 308 to the sequencing chip 406 is generally in an upward-bulging water drop shape, the air flow can blow the sample on the sequencing chip 406 to change the upward-bulging water drop shape into a flat shape on the sequencing chip 406, and then after the reagent drops downwards, the air flow also blows the reagent and the sample, so that the contact area between the sample and the reagent can be increased, the sample and the reagent are uniformly mixed, the combination between the sample and the reagent is fast, and the sequencing period is further shortened;

then, after the cantilever 508 rotates to move the suction head 308 away from the sequencing chip 406, the second motor 524 operates to drive the piston 520 to move vertically upward by a predetermined distance b and a predetermined distance a for resetting.

In a preferred embodiment, as shown in fig. 13, the outer surface of the bottom cylinder shell 512 is provided with a sensing hole, and a pressure sensor 517 is arranged in the sensing hole, which means that in the process of moving the piston 520 downwards, the pressure change in the bottom cylinder shell 512 can be sensed by the pressure sensor 517, and according to the sensing result of the pressure sensor 517, whether the pipetting mechanism has an operation error can be determined.

As shown in fig. 3, 7 and 8, an imaging mechanism 600 is further installed in the outer casing 100, a display screen 102 is further disposed on the outer casing 100, and the imaging mechanism 600 is used for photographing the sequencing result on the sequencing chip 406 on the display screen 102 for the staff to view. Specifically, the imaging mechanism 600 includes a linear module f601 and a linear module g 602. The linear module f601 comprises a screw rod f and a motor f which are mounted on a guide support d and are in power connection, the screw rod f is parallel to the screw rod d, a sliding seat f is mounted on external threads of the screw rod f, the sliding seat f and the guide support d form sliding fit with the guide direction parallel to the axial direction of the screw rod f, and when the motor f operates to drive the screw rod f to rotate, the sliding seat f displaces along the axial direction of the screw rod f.

The linear module g602 comprises a guide support g connected with a sliding seat f, a screw rod g and a motor g are mounted on the guide support g, the guide support g and the screw rod g are in power connection, the axial direction of the screw rod g and the guide direction of the guide support g are all parallel to the axial direction of the screw rod a, the sliding seat g is mounted on the external thread of the screw rod g, the sliding seat g and the guide support g form sliding fit, the sliding seat f moves to drive the linear module g602 to move together, in addition, when the motor g operates to drive the screw rod g to rotate, the sliding seat g displaces along the guide direction of the guide support g.

A fixed frame 603 is arranged on the sliding seat g, a main rod 604 is vertically arranged at the bottom of the fixed frame 603, a branch rod 605 is arranged outside the main rod 604, the extension direction of the branch rod 605 is parallel to the axial direction of the screw rod g, and a fluorescence scanner 606 is arranged at the suspension end of the branch rod 605.

After the reagent on the sequencing chip 406 is added, straight line module f601 and straight line module g602 cooperate the operation, make fluorescence scanner 606 be located sequencing chip 406 directly over, fluorescence scanner 606 scans sequencing chip 406 and takes a picture to the sequencing result and transmits for display screen 102 on, supply the staff to watch, in addition, fluorescence scanner 606 can realize for prior art, does not describe repeatedly.

As shown in fig. 7, a heating element 700 is further disposed in the outer casing 100, which is significant in that the optimal temperature for combining the sample with different reagents is different, so that the heating element 700 is disposed to adjust the temperature in the outer casing 100, and the heating element 700 can be implemented by the existing heating technology, and is not described again.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. The utility model provides a gene sequencer, its includes shell body (100), and the lateral wall of shell body (100) is seted up jaggedly, and breach department matches and installs closing cap (101), its characterized in that: a storage mechanism a (200), a storage mechanism b (300) and a storage mechanism c (400) are arranged in the outer shell (100), the storage mechanism a (200) is used for storing a reagent box (205), a sample and a reagent are stored in the reagent box (205), the storage mechanism b (300) is used for storing a suction head (308), and the storage mechanism c (400) is used for storing a sequencing chip (406);

still be provided with draw gear (500) in shell body (100), draw gear (500) include drive mechanism and move liquid mechanism, and the detachable connection between drive mechanism's the end of pulling and suction head (308), drive mechanism are used for pulling suction head (308) and take place to remove between kit (205) and sequencing chip (406), move liquid mechanism and utilize suction head (308) to realize that sample or reagent ration in kit (205) drips to sequencing chip (406).

2. A gene sequencer according to claim 1, wherein: the storage mechanism a (200) comprises a linear module a (201) and an upright post (202), the linear module a (201) is used for driving the upright post (202) to move, the moving direction of the linear module a is perpendicular to the side wall of the outer shell (100) with the notch, a tray (203) which is horizontally arranged is installed on the upright post (202), and a baffle plate (204) is arranged at one end, away from the notch, of the tray (203);

the reagent box (205) is matched with the tray (203) in a detachable sliding guide mode through the guide piece, a plurality of groups of test tube holes are formed in the upper end face of the reagent box (205) at even intervals, test tubes are arranged in the test tube holes, and samples or reagents are filled in the test tubes.

3. A gene sequencer according to claim 2, wherein: storage mechanism c (400) is including installing grudging post (401) in shell body (100), grudging post (401) are close to the breach, install on grudging post (401) and are tray table (402) that the level was arranged, the up end of tray table (402) is provided with the mounting groove and the mounting groove runs through tray table (402) along stand (202) moving direction's both sides face, slidable mounting has back pole (403) in the mounting groove, be provided with on grudging post (401) and be used for driving about back pole (403) and take place gliding sharp module b (404).

4. A gene sequencer according to claim 3, wherein: the storage mechanism b (300) is positioned between the storage mechanism a (200) and the storage mechanism c (400), the storage mechanism b (300) comprises an installation frame (301) fixedly arranged at the gap, the installation frame (301) is in a rectangular ring shape, a buckling frame (302) is detachably arranged in the installation frame (301), and a storage part is arranged on the side surface, facing the inner cavity of the outer shell (100), of the buckling frame (302);

the storage component comprises a support rod (303) and a limiting plate (304) which are vertically arranged on the buckle frame (302), the side surface of the support rod (303) departing from the buckle frame (302) is provided with an installation opening running through the height of the support rod, the side wall of the installation opening facing the buckle frame (302) is an arc-shaped hole bottom, the upper end surface of the support rod (303) is provided with a support groove b (306) coaxially arranged with the hole bottom of the installation opening, two hole walls of the installation opening are respectively provided with a support groove a (305) in an arc groove shape, two groups of support grooves a (305) are coaxially arranged, and an axial line is parallel to an axial line of the support groove b (306);

the limiting plate (304) is positioned above the supporting rod (303), the supporting groove a (305) is positioned on one side, away from the buckling frame (302), of the limiting plate (304), an avoiding opening (307) penetrating through the height of the limiting plate is formed in the side face, away from the buckling frame (302), of the limiting plate (304), the side wall, facing towards the buckling frame (302), of the avoiding opening (307) is an arc-shaped hole bottom, and the hole bottom of the avoiding opening (307) and the hole bottom of the mounting opening are coaxially arranged;

the support groove a (305) and the support groove b (306) are equal in diameter, the hole bottom of the avoidance opening (307) and the hole bottom of the installation opening are equal in diameter, the hole bottom diameter of the installation opening is smaller than that of the support groove b (306), an external step is arranged at the upper opening end of the suction head (308), the diameter of the suction head (308) is smaller than that of the hole bottom of the installation opening, and in an initial state, the suction head (308) is placed at the support groove a (305) through the matching of the external step and the support groove a (305).

5. A gene sequencer according to claim 4, wherein: the traction mechanism comprises a linear module c (501), a linear module d (502) and a linear module e (503), wherein an installation rack (504) and a support bracket (510) are arranged at the output end of the linear module e (503), and the linear module c (501), the linear module d (502) and the linear module e (503) are matched to drive the installation rack (504) and the support bracket (510) to displace in a three-dimensional coordinate system.

6. A gene sequencer according to claim 5, wherein: a spindle (505) which is vertically arranged is arranged on the mounting rack (504), a motor I (506) is mounted on the mounting rack (504), a worm gear (507) is arranged between the output end of the motor I (506) and the spindle (505), the worm gear (507) comprises a worm arranged at the output end of the motor I (506) and a worm wheel coaxially sleeved outside the spindle (505) through a bearing, a cantilever (508) is arranged outside a worm wheel shaft of the worm wheel along the radial direction, and a clamping connector (509) is vertically arranged at the bottom of the suspension end of the cantilever (508) downwards;

the sample position in the kit (205), the position of the sequencing chip (406), and the position of the tip (308) have circumscribed circle diameters equal to the distance between the axial line of the bayonet joint (509) and the axial line of the spindle (505).

7. A gene sequencer according to claim 6, wherein: the liquid transfer mechanism comprises a barrel shell vertically arranged on a support bracket (510), and the barrel shell is divided into three sections along the vertical direction and sequentially comprises a bottom barrel shell (512), a middle barrel shell (513) and an upper barrel shell (514) from bottom to top;

the upper end and the lower end of the upper cylinder shell (514) are opened, the upper opening end is provided with a second motor (524) through a motor frame, the output end of the second motor (524) is provided with a first screw rod (522) through a coupler, and the first screw rod (522) is coaxially positioned in the upper cylinder shell (514);

the upper end of the middle cylinder shell (513) is open, the lower end of the middle cylinder shell is closed, the upper opening end of the middle cylinder shell is coaxially fixed with the lower opening end of the upper cylinder shell (514), a piston (520) is arranged in the middle cylinder shell in a sliding mode, the upper end face of the piston (520) is coaxially provided with a piston rod (521), the piston rod (521) is hollow in the middle cylinder shell, the top end of the piston rod (521) extends into the upper cylinder shell (514) and is in threaded connection with the first screw rod (522), and the outer wall of the piston rod (521) is in sliding guide fit with the inner wall of the upper cylinder shell (514) in the vertical direction through a sliding piece;

connecting sleeve (518) has been extended to the coaxial extension of the lower blind end of well shell (513), the upper end opening of end shell (512), the lower extreme seals and coaxial cover establishes in the outside of connecting sleeve (518), connecting hole (519) that are used for intercommunication between well shell (513) and end shell (512) are seted up to the lower blind end of well shell (513), the coaxial extension of blind end of end shell (512) has connection mouth (515), the top of connecting mouth (515) stretches into in end shell (512) and is provided with rubber cap (516), realize the intercommunication through connecting pipe (511) between bottom and joint (509).

8. A gene sequencer according to claim 7, wherein: an imaging mechanism (600) is further installed in the outer shell (100), and a display screen (102) is further arranged on the outer shell (100);

the imaging mechanism (600) comprises a straight line module f (601), a straight line module g (602) and a fluorescence scanner (606), wherein the straight line module f (601) and the straight line module g (602) are matched for driving the fluorescence scanner (606) to be positioned right above the sequencing chip (406) or far away from the sequencing chip (406), and the fluorescence scanner (606) is used for scanning sequencing results on the sequencing chip (406) and taking pictures to transmit the pictures to the display screen (102);

a heating element (700) is also arranged in the outer shell (100).

9. A method of using a gene sequencer according to claim 8, wherein: four reagents are arranged in the reagent kit (205), and the sample and the four reagents in the reagent kit (205) sequentially comprise the following components along the direction from the storage mechanism a (200) to the storage mechanism c (400): a sample, reagent a, reagent b, reagent c, and reagent d;

which comprises the following steps:

the method comprises the following steps: the position of the sample, the position of the sequencing chip (406) and the position of the suction head (308) are named as a point a;

the straight line module d (502) and the straight line module e (503) are matched to drive the main shaft (505) to move, so that the point a is positioned on the axis line of the main shaft (505);

the first motor (506) operates to drive the cantilever (508) to rotate, so that the clamping joint (509) is positioned right above the suction head (308);

the linear module c (501) operates to drive the clamping joint (509) to move downwards so that the suction head (308) is clamped on the clamping joint (509), and the linear module c (501) operates to drive the clamping joint (509) to move upwards so that the suction head (308) leaves the storage component;

step two: the first motor (506) operates to drive the cantilever (508) to rotate, so that the sucker (308) is positioned right above the sample;

the operation of the linear module c (501) drives the sucker (308) to move downwards to be inserted into a test tube filled with a sample, the operation of the liquid-transfering mechanism drives the sucker (308) to suck a quantitative sample, and the operation of the linear module c (501) drives the sucker (308) to move upwards to separate from the test tube;

the first motor (506) operates to drive the cantilever (508) to rotate, so that the sucker (308) is positioned right above the sequencing chip (406), and the pipetting mechanism operates to drip the sample in the sucker (308) downwards onto the sequencing chip (406);

step three: the central line of the connecting line between the position of the reagent a and the position of the sequencing chip (406) is a central line a, a point b exists on the central line a, the distance between the point b and the reagent a is equal to the distance between the point b and the sequencing chip (406), and the distance is equal to the distance between the axial line of the clamping joint (509) and the axial line of the spindle (505);

the straight line module d (502) and the straight line module e (503) are matched to drive the main shaft (505) to move, so that the point b is positioned on the axis line of the main shaft (505);

the first motor (506) operates to drive the cantilever (508) to rotate, so that the sucker (308) is positioned right above the reagent a;

the straight line module c (501) operates to drive the suction head (308) to move downwards to be inserted into a test tube filled with a reagent a, the pipetting mechanism operates to enable the suction head (308) to suck a quantitative reagent a, and the straight line module c (501) operates to drive the suction head (308) to move upwards to separate from the test tube;

the first motor (506) operates to drive the cantilever (508) to rotate, so that the sucker (308) is positioned right above the sequencing chip (406), and the pipetting mechanism operates to make the reagent a in the sucker (308) drop downwards onto the sequencing chip (406);

step four: the central line of a connecting line between the position of the reagent b and the position of the sequencing chip (406) is a central line b, a point c exists on the central line b, the distance between the point c and the reagent b is equal to the distance between the point c and the sequencing chip (406), and the distance is equal to the distance between the axial line of the clamping joint (509) and the axial line of the spindle (505);

repeating the third step to enable the point c to be positioned on the axis line of the main shaft (505), and realizing quantitative dripping of the reagent b on the sequencing chip (406);

step five: the central line of a connecting line between the position of the reagent c and the position of the sequencing chip (406) is a central line c, a point d exists on the central line c, the distance between the point d and the reagent c is equal to the distance between the point d and the sequencing chip (406), and the distance is equal to the distance between the axial line of the clamping joint (509) and the axial line of the spindle (505);

repeating the third step to enable the point d to be positioned on the axis line of the main shaft (505), and realizing quantitative dripping of the reagent c on the sequencing chip (406);

step six: the central line of a connecting line between the position of the reagent d and the position of the sequencing chip (406) is a central line d, a point e exists on the central line d, the distance between the point e and the reagent d is equal to the distance between the point e and the sequencing chip (406), and the distance is equal to the distance between the axial line of the clamping joint (509) and the axial line of the spindle (505);

and repeating the third step to enable the point e to be positioned on the axis line of the main shaft (505), and realizing the quantitative dripping of the reagent d onto the sequencing chip (406).

10. The method of using a gene sequencer according to claim 9, wherein: the pipetting mechanisms in the second step, the third step, the fourth step, the fifth step and the sixth step operate:

s1: when the suction head (308) is inserted into the test tube, the second motor (524) operates to drive the piston (520) to vertically move downwards for a preset distance a, so that the volume of the area between the piston (520) and the closed end of the middle cylinder shell (513) is reduced, the pressure is increased, the pressure in the bottom cylinder shell (512) is changed along with the reduction of the volume, and the outer surface of the rubber cap (516) is squeezed and shrunk by the pressure;

the second motor (524) operates to drive the piston (520) to move upwards by a preset distance a, so that the volume of the area between the piston (520) and the closed end of the middle barrel shell (513) is increased and recovered, the pressure is gradually reduced and recovered, the rubber cap (516) is gradually expanded and recovered, and finally the sample or the reagent in the test tube is quantitatively sucked into the suction head (308);

s2: when the sucker (308) is positioned right above the sequencing chip (406), the second motor (524) operates to drive the piston (520) to move downwards by a preset distance a, so that the sample or the reagent in the sucker (308) drops downwards onto the sequencing chip (406);

the second motor (524) operates to continuously drive the piston (520) to move vertically downwards for a preset distance b, in the process, the rubber cap (516) is continuously compressed, air in the suction head (308) is discharged downwards through the connecting pipe (511) to form an air flow, the air flow blows the inner wall of the suction head (308), residual samples or residual reagents are blown by the air flow to drip downwards onto the sequencing chip (406), the air flow blows the samples or reagents on the sequencing chip (406), the samples are evenly spread on the sequencing chip (406), and the samples and the reagents are evenly mixed;

after the cantilever (508) rotates to make the suction head (308) far away from the sequencing chip (406), the second motor (524) operates to drive the piston (520) to move vertically up by a preset distance b and a preset distance a for resetting.