CN115678744A - Nucleic acid extraction instrument - Google Patents
Nucleic acid extraction instrument Download PDFInfo
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- CN115678744A CN115678744A CN202211368059.7A CN202211368059A CN115678744A CN 115678744 A CN115678744 A CN 115678744A CN 202211368059 A CN202211368059 A CN 202211368059A CN 115678744 A CN115678744 A CN 115678744A
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- nucleic acid
- component
- acid extraction
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 66
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 66
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 66
- 238000000605 extraction Methods 0.000 title claims abstract description 47
- 230000033001 locomotion Effects 0.000 claims abstract description 93
- 239000011324 bead Substances 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims description 32
- 239000003153 chemical reaction reagent Substances 0.000 claims description 27
- 238000010438 heat treatment Methods 0.000 claims description 23
- 238000003825 pressing Methods 0.000 claims description 18
- 230000001174 ascending effect Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 230000005389 magnetism Effects 0.000 abstract description 21
- 239000007788 liquid Substances 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000003014 reinforcing effect Effects 0.000 description 9
- 238000005406 washing Methods 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 6
- 238000010828 elution Methods 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 5
- 230000000712 assembly Effects 0.000 description 4
- 238000000429 assembly Methods 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000013021 overheating Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
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Abstract
The invention discloses a nucleic acid extraction instrument which comprises a magnetic attraction component, a first movement component and a magnetic attraction switching component, wherein the magnetic attraction component comprises a magnetic component and a magnetic sleeve component; the first moving assembly comprises a first moving seat, the magnetic sleeve assembly and the magnetic assembly are respectively connected with the first moving seat, and the first moving assembly can drive the magnetic attraction assembly to descend along a first direction; magnetism is inhaled and is switched subassembly and connect first removal seat and magnetic component respectively, and magnetism is inhaled and is switched subassembly and can order about magnetic component and descend along first direction on first removal seat. Utilize first motion subassembly can take out the magnetic bead that adsorbs the nucleic acid molecule fast, and the magnetic bead surface remains the waste liquid few. The invention can be widely applied to the technical field of nucleic acid detection.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid extraction instrument.
Background
In nucleic acid detection analysis, nucleic acid samples need to be subjected to lysis mixing, washing and elution, and extraction and amplification are usually realized in a magnetic bead adsorption manner. Most of common nucleic acid extraction instruments are magnet-based, wherein magnetic beads of sample liquid in a test tube are adsorbed to one inner wall side of the test tube from the side surface, waste liquid is discharged from the test tube, the magnetic beads are left in the test tube, washing liquid or eluent is injected into the test tube, and washing or elution is carried out. This kind of extraction mode, annotate liquid, the flowing back is comparatively consuming time, leads to the efficiency of nucleic acid extraction and detection to be lower, and may remain the waste liquid in the test tube, and this also can influence the effect of extracting, detecting nucleic acid.
Disclosure of Invention
In order to solve at least one of the above technical problems, the present invention provides a nucleic acid extraction apparatus, which improves the efficiency of nucleic acid extraction, and adopts the following technical scheme.
The nucleic acid extraction instrument provided by the invention comprises a magnetic attraction component, a first motion component and a magnetic attraction switching component, wherein the magnetic attraction component comprises a magnetic component and a magnetic sleeve component, the magnetic component comprises a plurality of magnetic rods distributed in an array, the magnetic sleeve component comprises a plurality of magnetic sleeves distributed in an array, and magnetic beads can be adsorbed on the surfaces of the magnetic sleeves under the condition that the magnetic rods extend into the magnetic sleeves; the first movement assembly comprises a first movable seat capable of being lifted, the magnetic sleeve assembly and the magnetic assembly are respectively connected with the first movable seat, and under the driving of the first movement assembly, the magnetic attraction assembly can descend along a first direction, so that the magnetic sleeve can extend into a reagent; the magnetic attraction switching component is respectively connected with the first movable seat and the magnetic component, and under the driving of the magnetic attraction switching component, the magnetic component descends along the first direction on the first movable seat, so that the magnetic component extends into the magnetic sleeve.
In some embodiments of the present invention, the magnetic sleeve assembly includes a magnetic sleeve plate, the magnetic sleeve is connected to the magnetic sleeve plate, and the magnetic sleeve plate is detachably connected to the first movable seat.
In some embodiments of the present invention, the first movable base is provided with a slot into which the magnetic sleeve plate can be inserted.
In some embodiments of the present invention, the first moving assembly includes a pressing plate, the pressing plate is connected to the first moving seat, the slot is formed between the pressing plate and the first moving seat, and the pressing plate is provided with an avoiding area so that the magnetic rod can extend into the magnetic sleeve.
In some embodiments of the invention, the nucleic acid extraction apparatus includes a fourth motion assembly and a shutter, the shutter is connected to the fourth motion assembly, and the shutter moves to below the magnetic sleeve assembly under the driving of the fourth motion assembly.
In some embodiments, the nucleic acid extraction apparatus includes a second movement component, the first movement component is connected to the second movement component, and the first movement component and the magnetic attraction component can move along a second direction under the driving of the second movement component.
In some embodiments of the invention, the nucleic acid extraction apparatus includes a third motion assembly, the third motion assembly is connected to the second motion assembly, and the second motion assembly moves along a third direction under the driving of the third motion assembly, so as to drive the magnetic attraction assembly to move along the third direction.
In some embodiments of the present invention, the second motion assembly includes a second mounting base, the fourth motion assembly is connected to the second mounting base, the fourth motion assembly includes a fourth driver, the fourth driver is connected to the second mounting base, and the baffle is connected to the fourth driver, and the baffle is moved in the second direction by the fourth driver.
In some embodiments, the nucleic acid extraction apparatus is provided with at least three test tube areas, at least one of the test tube areas is provided with a heating element, and the magnetic attraction element can move to the upper part of any one of the test tube areas.
In some embodiments of the present invention, the magnetic attraction component can be repeatedly lifted and lowered at a set frequency under the driving of the first motion component, so that the magnetic sleeve stirs the reagent.
The embodiment of the invention has at least the following beneficial effects: inhale switching module's the down of driving about, the bar magnet can descend and stretch into the magnetism sleeve pipe, and first motion subassembly drives about magnetism and inhales the subassembly and descends to make the magnetism sleeve pipe stretch into reagent, under the magnetic attraction of bar magnet, the magnetic bead can adsorb on magnetism sleeve pipe surface, and first motion subassembly drives about magnetism and inhales the subassembly and rise, and the magnetic sleeve pipe is taking the magnetic bead to take out from the reagent, so that shift the magnetic bead to in washing liquid or the eluant. Utilize first motion subassembly can take out the magnetic bead that adsorbs the nucleic acid molecule fast, and the magnetic bead surface remains the waste liquid few. The invention can be widely applied to the technical field of nucleic acid detection.
Drawings
The described and/or additional aspects and advantages of embodiments of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings. It should be noted that the embodiments presented in the following figures are exemplary only and are not to be construed as limiting the invention.
FIG. 1 is a diagram of the magnetic attraction assembly, the magnetic attraction switching assembly, the first moving assembly, the second moving assembly, the third moving assembly and the fourth moving assembly of the nucleic acid extracting apparatus.
Figure 2 is an exploded view of the structure of figure 1 showing the heat shield, test tube section and heating assembly.
Fig. 3 is a structural diagram of the magnetic attraction component, the magnetic attraction switching component and the first moving component.
Fig. 4 is an exploded view of the structure of fig. 3.
Reference numerals:
1100. a magnetic component; 1200. a magnetic sleeve assembly; 1201. a magnetic sleeve plate; 1301. a magnetic attraction switching driver; 1302. a magnetic switching installation component;
2100. a first motion assembly; 2101. a first movable base; 2102. pressing a plate; 2103. a first mounting seat; 2104. a first driver; 2105. reinforcing the structure; 2200. a second motion assembly; 2300. a third motion assembly; 2400. a fourth motion assembly; 2401. a baffle plate;
3000. a test tube area; 3100. a heating assembly; 3200. an insulating panel.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
In the description of the present invention, it is to be understood that, if the terms "central", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. are used in the orientation or positional relationship indicated in the drawings, this is for convenience of description and simplicity of description only, and does not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention. The features defined as "first" and "second" are used to distinguish feature names rather than having a special meaning, and further, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The invention relates to a nucleic acid extraction instrument which comprises a magnetic suction assembly and a first motion assembly 2100, wherein the magnetic suction assembly is used for adsorbing magnetic beads in a reagent, the first motion assembly 2100 comprises a first movable seat 2101 capable of ascending and descending, and the magnetic suction assembly is connected with the first movable seat 2101. It will be appreciated that the magnetically attractive assembly is capable of being lowered in a first direction upon actuation of the first motion assembly 2100. Specifically, the subassembly of inhaling is inhaled to magnetism can stretch into in the reagent after the decline to magnetic bead in the adsorption agent or release the magnetic bead that adsorbs to the reagent in, if first motion subassembly drove the subassembly of inhaling to rise, then inhale subassembly and reagent separation.
Magnetism is inhaled the subassembly and is included magnetic assembly 1100 and magnetic sleeve subassembly 1200, and magnetic assembly 1100 includes the bar magnet, and the bar magnet has magnetism, and magnetic sleeve subassembly 1200 includes the magnetic sleeve pipe, and under the circumstances that the bar magnet stretched into the magnetic sleeve pipe, the magnetic bead can adsorb on the sheathed tube surface of magnetism. It can be understood that the magnetic sleeve replaces the magnetic rod to extend into the reagent to realize the adsorption of the magnetic beads, so that the magnetic rod can be prevented from being infected with the reagent. Further, the magnetic sleeve assembly 1200 and the magnetic assembly 1100 are respectively connected to the first moving seat 2101, and the magnetic sleeve can extend into or leave the reagent during the process that the first moving assembly 2100 drives the magnetic attraction assembly to descend or ascend along the first direction.
It will be appreciated that if the magnetic rod is removed from the magnetic sleeve, the beads are released from the magnetic sleeve and fall. Specifically, the nucleic acid extraction instrument includes a magnetic switch assembly, the magnetic switch assembly is connected to the first movable holder 2101 and the magnetic assembly 1100, and the magnetic assembly 1100 is driven by the magnetic switch assembly to move up and down along the first direction on the first movable holder 2101.
Specifically, in the case of a magnetic cannula projecting into the reagent: the magnetic attraction switching component drives the magnetic component 1100 to descend, the magnetic rod is inserted into the magnetic sleeve, and the magnetic beads in the reagent are adsorbed on the surface of the magnetic sleeve; switching components orders about magnetic component 1100 and rises to inhale to magnetism, and the bar magnet leaves from the magnetism sleeve, and then the magnetic bead drops from the surface release of magnetism sleeve, and perhaps the bar magnet is not taken out from the magnetism sleeve, but the bar magnet rises to can lose to have the position of magnetism to inhale the adsorption action to the magnetic bead. As an alternative, of course, it is also possible to provide: first, the magnetic bar is driven to be inserted into the magnetic sleeve and keep inserted by the magnetic switching assembly, and then the magnetic sleeve is driven to be inserted into the reagent by the first moving assembly 2100.
Regarding the adsorption of the magnetic rod and the magnetic sleeve to the magnetic beads: in some examples, the magnetic rod is inserted into the magnetic sleeve and then magnetizes the magnetic sleeve, and the magnetic sleeve has magnetism. In some examples, after the magnetic rod extends into the magnetic sleeve, the magnetic force of the magnetic rod can attract the magnetic beads through the magnetic sleeve.
Further, the magnetic assembly 1100 comprises a plurality of magnetic rods distributed in an array, and the magnetic sleeve assembly 1200 comprises a plurality of magnetic sleeves distributed in an array, wherein each magnetic rod corresponds to the position of each magnetic sleeve, so that a plurality of nucleic acid samples are processed, and the flux of the nucleic acid extraction instrument is expanded. Specifically, in the magnetic suction assembly, 92 magnetic rods and 92 magnetic sleeves are respectively arranged. With reference to the drawings, the magnetic assembly is provided with two sets of magnetic assemblies 1100 and two sets of magnetic sleeve assemblies 1200, which can detect 192 nucleic acid samples simultaneously.
As an embodiment, the magnetic attraction assembly can be driven by the first motion assembly 2100 to repeatedly move up and down at a set frequency, such that the magnetic sleeve agitates the reagent, increasing the agitation of the reagent, and facilitating lysis mixing or washing or elution. It is understood that, in this case, the magnetic rod may be inserted into the magnetic sleeve, or may be not inserted into the magnetic sleeve.
It can be understood that the bottom of the magnetic sleeve is sealed, and further, the bottom of the magnetic sleeve is designed to be a conical surface or a spherical surface, so that the resistance of the magnetic sleeve when the magnetic sleeve extends into a reagent can be reduced, splash of the magnetic sleeve when the magnetic sleeve extends into the reagent can be avoided, and the reagent is prevented from splashing out of the reagent tube.
In one embodiment, the first motion assembly 2100 includes a first mount 2103 and a first drive 2104, the first drive 2104 coupled to the first mount 2103, and the first movable mount 2101 coupled to the first drive 2104. It will be appreciated that the first movable mount 2101 can be raised and lowered in a first direction by actuation of the first drive 2104.
Specifically, the first driver 2104 includes a motor, and the first driver 2104 is drivingly connected to the first movable base 2101 by a ball screw pair. Of course, as an alternative, it is also possible to provide: the first driver 2104 is drivingly connected to the first movable holder 2101 via a timing belt; alternatively, the first driver 2104 includes a cylinder.
Further, first motion assembly 2100 includes a first guide member coupled to first mount 2103, the first guide member disposed along a first direction, and first movable mount 2101 slidably coupled to the first guide member. Specifically, the first guide member is configured as a guide rail, and the first movable holder 2101 is slidably connected to the first guide member by a slider.
In some examples, in the case that the first guiding member is configured as a guide rail, the first guiding member is configured as two, and the two first guiding members are disposed on two mounting surfaces of the first mounting seat 2103 in different directions, and the two first guiding members limit the slider which is clamped with the first movable seat 2101 from different directions, so as to prevent the first movable seat 2101 from shifting during lifting and ensure that the magnetic attraction assembly is accurately moved in place. Specifically, the first mount 2103 is provided with first guide members on two vertical surfaces at right angles to each other, respectively.
With regard to the first guide part, in some examples, it may alternatively be provided that: the first guide member is provided as a guide bar, and the first movable base 2101 is provided with a sleeve or a linear bearing slidably coupled with the guide bar.
In one embodiment, the nucleic acid extracting apparatus includes a second moving unit 2200, and the first moving unit 2100 is connected to the second moving unit 2200. Specifically, upon actuation of the second motion assembly 2200, the first motion assembly 2100 and the magnetically attractive assembly are capable of moving in a second direction. It can be understood that, in combination with the driving of the first motion assembly 2100 and the second motion assembly 2200, the magnetic attraction assembly can achieve translation and lifting, and achieve movement of the magnetic attraction assembly in two dimensions, so that the magnetic attraction assembly can move to a plurality of set positions in the nucleic acid extraction instrument, and complete lysis mixing, washing and elution of nucleic acid.
Specifically, first mount 2103 is coupled to second kinematic assembly 2200, second kinematic assembly 2200 includes a second driver, and first mount 2103 is coupled to the second driver, and with reference to the figures, second kinematic assembly 2200 includes a second mount, and the second driver is coupled to the second mount. It will be appreciated that the first mount 2103 moves in the second direction upon actuation of the second actuator to effect movement of the first motion assembly 2100 and magnetically attractive assembly in the second direction.
In some examples, the second drive includes a motor, and the second drive is drivingly coupled to the first mount 2103 via a ball screw pair. As an alternative, it is of course also possible to provide: the second driver is in transmission connection with the first mounting seat 2103 through a synchronous belt; alternatively, the second actuator comprises a pneumatic cylinder.
Further, second motion assembly 2200 includes at least one second guide member coupled to a second mount, the second guide member being disposed along the second direction, and first mount 2103 slidably coupled to the second guide member. Specifically, the second guide member is provided as a guide rail, and the first mount 2103 is slidably connected to the second guide member via a slider.
Of course, in some examples, the second guiding component may alternatively be designed as a guide rod, and the first mounting seat 2103 may be provided with a bushing or a linear bearing slidably connected with the guide rod.
As an embodiment, the nucleic acid extracting apparatus includes a third motion unit 2300, and the third motion unit 2300 is connected to the second motion unit 2200. Specifically, upon actuation of the third motion assembly 2300, the second motion assembly 2200 moves along the third direction, such that the first motion assembly 2100 moves along the third direction, thereby moving the magnetically attractive assembly along the third direction. It can be appreciated that, in combination with the actuation of the first motion assembly 2100, the second motion assembly 2200, and the third motion assembly 2300, the magnetically attractive assembly can achieve lifting and translation in two directions, thereby achieving movement of the magnetically attractive assembly in three dimensions so as to expand the range of movement of the magnetically attractive assembly.
Specifically, the second mount is connected to the third motion assembly 2300, the third motion assembly 2300 includes a third driver and a third mount, the third driver is connected to the third mount, and the second mount is connected to the third driver. It will be appreciated that upon actuation of the third actuator, the second mount moves in a third direction, thereby effecting movement of the second motion assembly 2200, the first motion assembly 2100 and the magnetically attractive assembly in the third direction.
In some examples, the third driver includes a motor, and the third driver is in transmission connection with the second mounting seat through a ball screw pair. As an alternative, it is of course also possible to provide: the third driver is in transmission connection with the second mounting seat through a synchronous belt; alternatively, the third actuator comprises a pneumatic cylinder.
Further, the nucleic acid extracting apparatus includes a third guide part or the third motion unit 2300 includes a third guide part, the third guide part being provided at least one, the third guide part being provided in the third direction. It will be appreciated that the second mount moves along the third guide member under the urging of the third actuator.
Specifically, the third guide part is provided as a guide rail, and the second mounting seat is connected with the third guide part in a sliding manner through a sliding block. In some examples, both ends of the second mounting seat are respectively connected to the third guide member through a support having a slider. Of course, as an alternative, the third guide element can also be designed as a guide rod, the second mounting socket being provided with a bushing or a linear bearing.
In one embodiment, the nucleic acid extracting apparatus is provided with at least three test tube zones 3000, the test tube zone 3000 is provided with a deep-well plate having a plurality of well sites, the reagent tubes can be placed in different test tube zones 3000, and the reagent tubes contain lysis solution or washing solution or eluent. It is to be understood that in the case where the test tube section 3000 is provided in at least four, the test tube section 3000 for washing or elution may be provided in at least two.
It will be appreciated that the magnetic assembly can be moved over any of the tube sections 3000. In some examples, each cuvette zone 3000 is arranged in an array, in this case, the first direction, the second direction and the third direction are designed to be perpendicular to each other, so as to realize the movement of the magnetic attraction component in a three-dimensional space, and the magnetic attraction component can move back and forth, left and right, and up and down in the nucleic acid extraction instrument.
Further, a heating assembly 3100 is disposed in the at least one test tube zone 3000, and the heating assembly 3100 is capable of heating the reagent so as to meet the temperature requirements of the biological detection. Referring to the drawings, the test tube zones 3000 are provided in four, wherein the heating assembly 3100 is provided in two test tube zones 3000, and two test tube zones 3000 having the heating assembly 3100 are provided in diagonal positions.
Specifically, heating assembly 3100 includes a heating block coupled to a deep hole plate, and the heating block has hole sites corresponding to the hole sites of the deep hole plate. Further, the heating assembly 3100 comprises heating films, which are connected to heating blocks, and which are used to separate the heating blocks in different heating assemblies 3100, avoiding temperature interference.
In some examples, in the case of the nucleic acid extraction instrument, the bottom is provided with a heat insulating plate 3200, the heat insulating plate 3200 is provided as a fiber plate, and the heat insulating plate 3200 is used to insulate heat of the heating element 3100 and prevent the bottom of the nucleic acid extraction instrument from overheating.
In some examples, the cuvette zone 3000 is not provided with the heating assembly 3100, and the nucleic acid extraction instrument performs a normothermic processing of the nucleic acid sample. Of course, it can be understood that, in the case that the heating assembly 3100 is provided in the test tube zone 3000, if the heating assembly 3100 is not turned on in the test tube zone 3000, the nucleic acid extraction apparatus can also perform the normal temperature processing on the nucleic acid sample, and the user can turn on the heating assembly 3100 according to actual needs.
Regarding the movement of the magnetic attraction component, as an alternative, it can also be designed as: the nucleic acid extraction instrument does not have the third motion assembly 2300, but the first motion assembly 2100 is used to raise and lower the magnetic assembly in the first direction and the second motion assembly 2200 is used to translate the magnetic assembly in the second direction. Accordingly, in this case, the test tube zones 3000 are distributed in the second direction.
As an embodiment, the nucleic acid extraction instrument includes baffle 2401, and the magnetism sleeve in the subassembly is inhaled to magnetism adsorbs the magnetic bead and rises the back, and the subassembly translation in-process is inhaled to magnetism, for preventing that the magnetic bead from dropping in the nucleic acid extraction instrument or dropping to other reagents, utilizes baffle 2401 to inhale the below of subassembly and plays the guard action, avoids causing the pollution. Further, a rib is provided at the edge of the baffle 2401, and the rib forms a concave region on the upper side of the baffle 2401, so that the magnetic beads can be prevented from rolling off the baffle 2401 if the magnetic beads fall on the baffle 2401.
It is understood that the shield 2401 can be moved to and away from the underside of the magnetic sleeve assembly 1200. Specifically, the nucleic acid isolation apparatus includes a fourth movement unit 2100, and a shutter 2401 is connected to the fourth movement unit 2100, and the shutter 2401 moves below the magnetic hub unit 1200 by the actuation of the fourth movement unit 2100.
In some examples, the fourth drive includes a motor, and the fourth drive is drivingly connected to the fourth moving member through a ball screw pair. Of course, as an alternative, it is also possible to provide: the fourth driver is in transmission connection with the fourth moving part through a synchronous belt; alternatively, the fourth drive comprises a cylinder.
It will be appreciated that the shield 2401 and magnetically attracted assembly should be maintained in synchronous translation. Specifically, the fourth moving assembly 2100 is connected to the second mounting base, and under the driving of the third moving assembly 2300, the second mounting base drives the second moving assembly 2200, the first moving assembly 2100, the magnetic attraction assembly and the fourth moving assembly 2100 to synchronously translate along the third direction.
Further, a fourth driver is connected to the second mounting seat, and when the second moving assembly 2200 drives the first moving assembly 2100 and the magnetic attraction assembly to move along the second direction, the fourth driver drives the blocking plate 2401 to move along the second direction synchronously.
In order to make the fourth driver move the blocking plate 2401 smoothly, the fourth moving assembly 2100 includes a fourth guiding member, and the fourth moving member is slidably connected to the fourth guiding member. Further, under the condition that the design baffle 2401 moves synchronously with the magnetic attraction assembly along the second direction, the fourth guide part is arranged along the second movement direction and is connected with the second mounting seat.
In some examples, the fourth guide member is provided as a guide rail, and the fourth moving member is connected to the fourth guide member by a slider. Of course, an alternative design is also possible: the fourth guide part is arranged as a guide rod, and the fourth moving part is provided with a shaft sleeve or a linear bearing.
As an embodiment, the magnetic switching component includes a magnetic switching actuator 1301 and a magnetic switching mounting member 1302, the magnetic switching mounting member 1302 is configured as a mounting plate, a magnetic rod is connected to the magnetic switching mounting member 1302, the magnetic switching mounting member 1302 is connected to the magnetic switching actuator 1301, and the magnetic switching actuator 1301 is connected to the first movable base 2101.
Specifically, the magnetic switching actuator 1301 includes a motor, and the magnetic switching actuator 1301 is in transmission connection with the magnetic switching mounting part 1302 through a ball screw pair. Of course, as an alternative, it is also possible to provide: the magnetic attraction switching driver 1301 is in transmission connection with the magnetic attraction switching installation component 1302 through a synchronous belt; alternatively, the magnetic switching actuator 1301 includes a pneumatic cylinder.
Further, the switching component includes a magnetic switch guiding component, the magnetic switch guiding component is connected to the first movable base 2101, the magnetic switch guiding component is disposed along the first direction, and the magnetic switch mounting component 1302 is slidably connected to the magnetic switch guiding component.
In some examples, the magnetic switching guide member is configured as a guide rail, and the magnetic switching mounting member 1302 is slidably connected to the magnetic switching guide member through a slider, and specifically, the magnetic switching mounting member 1302 is connected to the magnetic switching guide member through a guide rail seat provided with a slider. In some examples, the magnetically attractive switching guide member may alternatively be designed as a guide rod, and the magnetically attractive switching mounting member 1302 or the guide rail seat is provided with a bushing or a linear bearing.
As an implementation mode, the magnetic sleeve in the magnetic attraction component is designed to be replaceable, so that when the nucleic acid extraction instrument processes the next batch of nucleic acid samples, a new magnetic sleeve can be used, and the nucleic acid samples are prevented from being polluted.
Specifically, the magnetic sleeve assembly 1200 includes a magnetic sleeve plate 1201, the magnetic sleeve plate 1201 is detachably connected to the first moving seat 2101, and the magnetic sleeve is connected to the magnetic sleeve plate 1201, and specifically, the magnetic sleeve is integrally formed with the magnetic sleeve plate 1201. It is understood that after the nucleic acid extraction is completed, the magnetic sleeve plate 1201 can be removed and replaced with a new magnetic sleeve.
The first movable seat 2101 is provided with a slot into which the magnetic sleeve plate 1201 can be inserted, so that the magnetic sleeve plate 1201 can be easily mounted and dismounted on the first movable seat 2101. Referring to the drawings, the first moving assembly 2100 includes a pressing plate 2102, and the pressing plate 2102 is connected to the first moving seat 2101, and particularly, the pressing plate 2102 is connected to the first moving seat 2101 through a screw or a bolt. It is understood that a slot is formed between the pressing plate 2102 and the first movable holder 2101, and particularly, the pressing plate 2102 is located at an upper side of the first movable holder 2101, a lower side of the pressing plate 2102 is a top surface of an inner wall of the slot, and a lower side of the first movable holder 2101 is a bottom surface of the inner wall of the slot.
With reference to the drawings, the pressing plate 2102 is provided with an avoidance area, the avoidance area penetrates through the pressing plate 2102, and the magnetic sleeve is located in the avoidance area, so that the magnetic rod can extend into the magnetic sleeve.
In some examples, the underside of the pressure plate 2102 is provided with a cushion structure, which helps the pressure plate 2102 to press and fix the magnetic sleeve plate 1201, and can prevent the pressure plate 2102 from crushing the magnetic sleeve plate 1201.
Further, the first moving assembly 2100 comprises a reinforcing structure 2105, the reinforcing structure 2105 is configured as a side plate, the reinforcing structure 2105 is connected with the side surface of the first moving seat 2101, the reinforcing structure 2105 is provided with a reinforcing surface for abutting against the upper side surface of the pressure plate 2102, and the flatness of the magnetic sleeve plate 1201 and the verticality of the magnetic sleeve are ensured by using the reinforcing surface, so that the accurate alignment of the magnetic rod, the magnetic sleeve and the reagent tube is ensured. Specifically, the reinforcing structure 2105 is coupled to the first movable seat 2101 by a screw or a bolt, and the reinforcing surface presses the upper side surface of the pressure plate 2102 to reinforce the coupling of the pressure plate 2102 to the first movable seat 2101.
As an embodiment, the nucleic acid extraction instrument includes a sterilizing part, and the sterilizing part is disposed in a cabinet of the nucleic acid extraction instrument. Further, the killing part is connected with the second mounting seat, and specifically, the killing part is arranged on the lower side surface of the second mounting seat. In some examples, the sterilizing component includes an ultraviolet lamp.
Regarding the switching of magnetism subassembly in each test tube district 3000 position, as the alternative, still can design: the nucleic acid extraction instrument comprises a turntable, 3000 test tube zones are distributed on the turntable along the circumference, the magnetic attraction component is positioned above the turntable, and the turntable is rotated to enable the 3000 test tube zone to rotate to the lower part of the magnetic attraction component.
The present invention will be described in detail below with specific examples, and it should be noted that the following descriptions are only illustrative and not specific limitations of the present invention.
The nucleic acid extraction instrument comprises a chassis, and a magnetic attraction assembly, a magnetic attraction switching assembly, a first motion assembly 2100, a second motion assembly 2200, a third motion assembly 2300 and a fourth motion assembly 2100 arranged in the chassis. Referring to the drawings, the first direction corresponds to a vertical direction, the second direction corresponds to a left-right direction, and the third direction corresponds to a front-back direction, and the fourth moving assembly 2100 can drive the blocking plate 2401 to move along the second direction.
Referring to the drawings, four test tube zones 3000 are arranged in an array, wherein two diagonal test tube zones 3000 are provided with heating assemblies 3100, a first moving seat 2101 is positioned above the test tube zone 3000, a second moving assembly 2200 is positioned above the first moving seat 2101, and a third moving assembly 2300 is positioned above the second moving assembly 2200.
The magnetic attraction assembly comprises 92 magnetic rods distributed in an array and 92 magnetic sleeves distributed in an array, and the magnetic sleeve plate 1201 is detachably replaced in a mode of being inserted into a slot between the pressing plate 2102 and the first movable holder 2101.
In the description herein, references to the terms "one embodiment," "some examples," "some embodiments," "illustrative embodiments," "examples," "specific examples," or "some examples" or the like, if any, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
While the embodiments of the present invention have been described in detail with reference to the drawings, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
In the description of the present invention, the appearances of the patent names "and" in the patent specification are the same as each other, but the patent names "and" are not necessarily the same as each other. For example, the patent names "a and B" indicate that the claimed content of the invention is: the technical scheme with the subject name of A and the technical scheme with the subject name of B.
Claims (10)
1. A nucleic acid extraction apparatus, characterized in that: comprises that
The magnetic attraction component comprises a magnetic component (1100) and a magnetic sleeve component (1200), the magnetic component (1100) comprises a plurality of magnetic rods distributed in an array, the magnetic sleeve component (1200) comprises a plurality of magnetic sleeves distributed in an array, and magnetic beads can be adsorbed on the surface of the magnetic sleeves when the magnetic rods extend into the magnetic sleeves;
the first moving assembly (2100), the first moving assembly (2100) comprises a first movable base (2101) capable of ascending and descending, the magnetic sleeve assembly (1200) and the magnetic assembly (1100) are respectively connected with the first movable base (2101), and the magnetic attraction assembly can descend along a first direction under the driving of the first moving assembly (2100) so that the magnetic sleeve can extend into a reagent;
the magnetic switching component is respectively connected with the first movable seat (2101) and the magnetic component (1100), and under the driving of the magnetic switching component, the magnetic component (1100) descends along the first direction on the first movable seat (2101) so as to enable the magnetic component (1100) to extend into the magnetic sleeve.
2. The nucleic acid extraction apparatus according to claim 1, wherein: the magnetic sleeve assembly (1200) comprises a magnetic sleeve plate (1201), the magnetic sleeve is connected with the magnetic sleeve plate (1201), and the magnetic sleeve plate (1201) is detachably connected with the first movable seat (2101).
3. The nucleic acid extraction apparatus according to claim 2, wherein: the first movable seat (2101) is provided with a slot into which the magnetic sleeve plate (1201) can be inserted.
4. The nucleic acid extraction apparatus according to claim 3, wherein: the first moving assembly (2100) comprises a pressing plate (2102), the pressing plate (2102) is connected with the first moving seat (2101), a slot is formed between the pressing plate (2102) and the first moving seat (2101), and an avoidance area is arranged on the pressing plate (2102) so that the magnetic rod can extend into the magnetic sleeve.
5. The nucleic acid extraction apparatus according to any one of claims 1 to 4, wherein: the nucleic acid extraction instrument comprises a fourth motion assembly (2400) and a baffle plate (2401), wherein the baffle plate (2401) is connected with the fourth motion assembly (2400), and the baffle plate (2401) moves to the position below the magnetic sleeve assembly (1200) under the driving of the fourth motion assembly (2400).
6. The nucleic acid extraction apparatus according to claim 5, wherein: the nucleic acid extraction instrument comprises a second motion assembly (2200), the first motion assembly (2100) is connected with the second motion assembly (2200), and the first motion assembly (2100) and the magnetic attraction assembly can move along a second direction under the driving of the second motion assembly (2200).
7. The nucleic acid extraction apparatus according to claim 6, wherein: the nucleic acid extraction instrument comprises a third motion assembly (2300), wherein the third motion assembly (2300) is connected with the second motion assembly (2200), and the second motion assembly (2200) moves along a third direction under the driving of the third motion assembly (2300) so as to drive the magnetic attraction assembly to move along the third direction.
8. The nucleic acid extraction apparatus according to claim 6, wherein: the second motion assembly (2200) comprises a second mounting seat, the fourth motion assembly (2400) is connected with the second mounting seat, the fourth motion assembly (2400) comprises a fourth driver, the fourth driver is connected with the second mounting seat, the baffle plate (2401) is connected with the fourth driver, and the baffle plate (2401) moves along the second direction under the driving of the fourth driver.
9. The nucleic acid extraction apparatus according to any one of claims 1 to 4, wherein: the nucleic acid extraction instrument is provided with at least three test tube areas (3000), at least one test tube area (3000) is provided with a heating assembly (3100), and the magnetic attraction assembly can move to the position above any one test tube area (3000).
10. The nucleic acid extraction apparatus according to any one of claims 1 to 4, wherein: driven by the first motion assembly (2100), the magnetic attraction assembly can be repeatedly lifted and lowered at a set frequency to enable the magnetic sleeve to stir the reagent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211368059.7A CN115678744A (en) | 2022-11-03 | 2022-11-03 | Nucleic acid extraction instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211368059.7A CN115678744A (en) | 2022-11-03 | 2022-11-03 | Nucleic acid extraction instrument |
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