CN220854895U - Full-automatic immunofluorescence analyzer - Google Patents

Abstract

The utility model relates to the technical field of fluorescence immunoassay devices, in particular to a full-automatic immunofluorescence analyzer, which comprises a rack, and a sample buffer liquid-homogenizing mechanism, a reagent card discharging mechanism and a fluorescence analysis mechanism which are arranged on the rack, wherein the sample buffer liquid-homogenizing mechanism comprises a sample module, a suction head supply module, a buffer liquid supply module, a mixing module and a liquid-transferring mechanism; the reagent card discharging mechanism comprises a card storing mechanism, a discharging transverse moving mechanism and a material stirring mechanism. The fluorescence analysis mechanism comprises an incubation module, a ferrying mechanism and a detection mechanism. Compared with the prior art, the analyzer can automatically load blood samples, automatically transfer reagent cards and automatically detect. Furthermore, because the elastic locating sleeve is inserted in the sample rack for placing the test tube, the test tube can be prevented from shaking, and the pipette can accurately sample when driving the suction head to sample, so that the detection accuracy is improved. The reagent card is directly pushed to move by the stirring pin of the stirring mechanism, so that the clamping type of the reagent card is simpler and easier to realize compared with the clamping type of the prior art.

Description

Full-automatic immunofluorescence analyzer

Technical Field

The utility model relates to the technical field of fluorescence immunoassay devices, in particular to a full-automatic immunofluorescence analyzer.

Background

POCT (point-of-CARE TESTING) is a subdivision industry of in vitro diagnostic devices (IVD), i.e. on-site sampling and instant analysis, which omits the complex procedure of specimen inspection in laboratory and rapidly obtains the inspection result.

When carrying out fluorescent immunity quantitative detection, most of traditional diagnostic instruments are semi-automatic detection instruments, the existing machine structure only comprises an optical path system and an electronic display system for quantitative detection, and the sample adding and chromatography processes are completed outside the machine manually; in use, firstly, the semi-automatic instrument performs sample loading outdoors, which is very unfavorable for some products with high temperature requirements; secondly, because the steps of manual sampling and sample adding are needed, quantitative analysis is carried out through an instrument, the generated test error of manual intervention is increased, the immunochromatography display time, the sample adding precision and the like are inaccurate, the test speed is low, and the manual sample adding is easy to cause biological pollution.

Therefore, the Chinese patent document with the publication number of CN204347046U discloses a full-automatic fluorescence immunoassay quantitative analysis device, and belongs to the field of quantitative fluorescence immunoassay detection. The immunity quantitative analysis device comprises a supporting bottom plate, a reagent strip storage and automatic loading module, a reaction plate module, a detection module, a sample adding module, a washing module and a control system, wherein the reagent strip storage and automatic loading module, the reaction plate module, the detection module, the sample adding module and the washing module are sequentially arranged on the supporting bottom plate, the reagent strip storage and automatic loading module provides reagent strips for the reaction plate module, the sample adding module adds samples in the sample module into the reaction plate module for reaction, and the samples enter the detection module for detection after the reaction is completed. The method solves the problem that in-vitro diagnosis products are difficult to realize automation, reduces human errors, improves the accuracy of testing and improves the detection efficiency.

Another example is chinese patent document CN208868936U, which discloses a kit transport device. This kit conveyor includes: the device comprises a kit storage assembly, a clamping mechanism, a conveying mechanism and a loading groove. The kit storage assembly includes a plurality of storage cavities for storing the kits. The clamping mechanism can clamp the kit out of the accommodating cavity and move along a first preset direction. The conveying mechanism is arranged along a direction perpendicular to the first preset direction. Through setting up and press from both sides and get the mechanism, press from both sides the kit that stacks in the kit storage component in proper order and take out and place in loading tank after, transport along the first direction of predetermineeing. The clamping mechanism can clamp the reagent box in any one of the accommodating grooves through the conveying mechanism, and the reagent box is conveyed in the direction perpendicular to the first preset direction. Compared with the prior art, the reagent kit conveying device does not need to manually place the reagent kit and push the reagent kit to a proper position, and realizes automatic conveying of the reagent kit.

On the one hand, the test tube at the sample module is easy to shake, so that errors are easy to generate when the sample adding module absorbs the blood sample, or the suction tube collides with the test tube or has larger friction. On the other hand, the extraction of the kit is carried out by clamping, and the structure is complex. Meanwhile, the inventor has more details to be improved in the prior art during research and development, so that the full-automatic immunofluorescence analyzer is provided based on the details.

Disclosure of utility model

Aiming at the technical problems in the prior art, the utility model provides a full-automatic immunofluorescence analyzer.

In order to achieve the above purpose, the present utility model provides the following technical solutions:

Providing a full-automatic immunofluorescence analyzer, comprising a rack, a sample buffer and liquid homogenizing mechanism, a reagent card discharging mechanism and a fluorescence analysis mechanism, wherein the sample buffer and liquid homogenizing mechanism, the reagent card discharging mechanism and the fluorescence analysis mechanism are arranged on the rack;

The sample buffer and liquid homogenizing mechanism comprises a sample module, a suction head supply module, a buffer liquid supply module, a mixing module and a liquid transferring mechanism; the sample module comprises a plurality of rows of sample frames, each row of sample frames is provided with a plurality of tube holes for placing test tubes, and the tube holes are inserted with elastic positioning sleeves which elastically prop against the outer walls of the test tubes; the suction head supply module comprises a suction head disc, and a plurality of placing holes for the suction heads to be inserted are formed in the suction head disc; the buffer liquid supply module comprises a liquid supply box with an upward opening; the mixing module comprises a liquid mixing disc, wherein the liquid mixing disc is provided with a plurality of mixing grooves with upward openings; the frame is also provided with a sample feeding station; the pipetting mechanism comprises a pipetting device and a pipetting drive module, and the pipetting drive module can drive the pipetting device to move among the sample module, the suction head supply module, the buffer liquid supply module, the mixing module and the sample loading station;

The reagent card discharging mechanism comprises a card storing mechanism, a discharging transverse moving mechanism, a material shifting mechanism and a sample discharging station, wherein the card storing mechanism is provided with a plurality of accommodating cavities for accommodating cartridge clip box bodies, the inside of the cartridge clip box bodies is provided with accommodating cavities for stacking and placing reagent cards, and the front side parts of the cartridge clip box bodies, which correspond to the bottom reagent cards, are provided with outlet openings; the material stirring mechanism comprises a material stirring seat, a material receiving seat which is fixed on the material stirring seat and provided with a material receiving groove, a material stirring sliding rail which is fixed on the material stirring seat, a material stirring pin which is slidably arranged on the material stirring sliding rail, and a material stirring driving mechanism for driving the material stirring pin to move on the material stirring sliding rail, and is used for pushing the bottom reagent card in the cartridge case forwards into the material receiving groove; the discharging transverse moving mechanism comprises a transverse moving slide rail arranged below the card storage mechanism, the material shifting seat is slidably matched on the transverse moving slide rail, and the material shifting slide rail is vertically arranged with the transverse moving slide rail; the discharging transverse moving mechanism further comprises a transverse moving driving mechanism for driving the material shifting seat to move along the transverse moving sliding rail so as to drive the material receiving seat to move between the plurality of cartridge box bodies and the sample loading station;

The fluorescence analysis mechanism comprises an incubation module, a transition mechanism and a detection mechanism, wherein the incubation module comprises an incubation seat, and the incubation seat is provided with a plurality of incubation cavities which are used for placing reagent cards loaded with samples and are arranged in parallel; the detection mechanism comprises a detection seat and an optical detection module, the detection seat is provided with a detection cavity for placing the reagent card to be detected, and the optical detection module is fixed on the detection seat to detect the reagent card to be detected; the transfer mechanism is used for moving the reagent cards in the plurality of incubation cavities to the detection cavity one by one.

Specifically, the sample module further comprises a positioning base, the top of the positioning base is provided with a plurality of positioning slide rails, the bottom of the sample frame is provided with positioning grooves, and the positioning grooves are in sliding fit with the positioning slide rails; the inner wall of the tube hole of the sample frame is provided with a longitudinally arranged dovetail groove, the elastic positioning sleeve is provided with a dovetail, and the dovetail groove are mutually matched in a sliding way; the elastic positioning sleeve comprises a ring sleeve and an elastic positioning arm extending towards the inside of the pipe hole, the elastic positioning arm is fixed on the ring sleeve, and the ring sleeve is matched with the inner wall of the pipe hole.

Specifically, the outlet of the cartridge clip box body is provided with an elastic sheet, the elastic sheet blocks the outlet, and the bottom reagent card of the cartridge clip box body is pushed by the material poking pin to jack the elastic sheet; the cartridge clip box body is also internally provided with a drying block body, and the drying block body presses the reagent card on the inner top layer of the cartridge clip box body.

Specifically, the ferrying mechanism comprises a ferrying displacement assembly, a traction assembly and a clamping assembly, wherein the traction assembly comprises a traction seat, a traction sliding rail, a traction pin and a traction driving assembly, the traction sliding rail is fixed on the traction seat, the traction pin is slidably matched on the traction sliding rail, the traction seat is provided with a ferrying cavity, and the traction driving assembly drives the traction pin to move on the traction sliding rail so as to clamp and move the reagent in the incubation cavity into the ferrying cavity;

The ferry displacement assembly comprises a ferry sliding rail and a ferry driving assembly, the ferry sliding rail is arranged along the parallel directions of the plurality of incubation cavities, the traction seat is slidably arranged on the ferry sliding rail, and the ferry driving assembly drives the traction seat to move along the ferry sliding rail, so that the ferry cavities are aligned with different incubation cavities and detection cavities;

The card feeding assembly is used for moving the reagent card in the ferrying cavity to the detection cavity.

Specifically, advance the card subassembly and include advance the card slide rail, trade the cassette and advance the card drive assembly, trade the cassette slidable fit on advancing the card slide rail, advance the card drive assembly drive and trade the cassette and slide along advancing the card slide rail.

Specifically, the card changing seat is provided with a pushing part capable of unidirectional elastic swinging, the reagent card is provided with a card feeding groove for embedding the pushing part, and the pushing part can be embedded into the card feeding groove forward to push the reagent card, and backward swinging can be separated from the card feeding groove.

Specifically, the end part of the reagent card is provided with a traction groove for clamping a traction pin; the end of the reagent card is provided with a positioning notch for embedding the stirring pin.

Specifically, the cavity wall of the incubation cavity is provided with an elastic pressing sheet.

Specifically, still be equipped with dry block in the cartridge clip box body, dry block pushes down its interior top layer's reagent card.

Specifically, the pipetting drive module comprises an X-axis slide rail, an X-axis drive mechanism, an arch door frame, a Y-axis slide rail, a Y-axis drive mechanism, a Z-axis slide rail and a Z-axis drive mechanism, wherein the X-axis slide rail is fixed on the frame, the arch door frame is slidably arranged on the X-axis slide rail, and the X-axis drive mechanism drives the arch door frame to move along the X axis; the Y-axis sliding rail is fixed on the arch portal, the Z-axis sliding rail is slidably arranged on the Y-axis sliding rail, and the Y-axis driving mechanism drives the Z-axis sliding rail to move along the Y axis; the liquid transfer device is slidably arranged on the Z-axis sliding rail, and the Z-axis driving mechanism drives the liquid transfer device to move along the Z axis; the X-axis driving mechanism, the Y-axis driving mechanism and the Z-axis driving mechanism all comprise a pipetting motor, a transmission wheel and a belt, and the pipetting motor drives the belt to move through the transmission wheel.

The utility model has the beneficial effects that:

Compared with the prior art, the full-automatic immunofluorescence analyzer not only can automatically add buffer solution and load samples, but also can avoid test tube shaking because the elastic positioning sleeve is inserted in the sample rack for placing the test tubes, and can accurately sample when the pipette drives the suction head to sample, thereby reducing the contact with the tube wall of the test tube and improving the detection accuracy.

The reagent card is directly pushed to move by the stirring pin of the stirring mechanism, so that the clamping type of the reagent card is simpler and easier to realize compared with the clamping type of the prior art.

The plurality of incubation cavities in parallel can make the reagent card have enough reaction time, just by the transition mechanism move to detection mechanism and detect, shift out the reagent card in the plurality of incubation cavities to detection cavity one by one through the transition mechanism, realize automatic reagent card that advances, and the transition mechanism further can insert the reagent card detection mechanism one by one, and the later reagent card can be with the preceding reagent card that has been detected forward extrusion, realize automatic reagent card that goes out.

Drawings

Fig. 1 is a schematic diagram of the internal structure of a full-automatic immunofluorescence analyzer in an embodiment.

Fig. 2 is a schematic structural diagram of a sample buffer homogenization mechanism in an embodiment.

Fig. 3 is a schematic structural view of a pipetting mechanism in the embodiment.

Fig. 4 is a schematic structural view of a row of sample holders of a sample module according to an embodiment, in which an elastic positioning sleeve is separated.

Fig. 5 is a schematic diagram of a card storing mechanism in an embodiment.

Fig. 6 is an exploded view of the cartridge body in the embodiment.

FIG. 7 is a schematic diagram of a reagent card in an embodiment.

Fig. 8 is a schematic diagram of a kick-out mechanism in an embodiment.

Fig. 9 is a schematic structural view of a backside view of a fluorescence analysis mechanism in an embodiment.

Fig. 10 is a schematic structural view of a traction assembly in a ferry mechanism in an embodiment.

Fig. 11 is a schematic structural view of the incubation module and the detection mechanism in the embodiment.

Fig. 12 is a schematic view of a part of the card feeding assembly in the embodiment.

Fig. 13 is a schematic diagram of a portion of an incubation module and detection mechanism in an embodiment.

Reference numerals:

The device comprises a frame 1, a sample loading station 11 and a suction head recycling cavity 12; reagent card 13, positioning notch 131, guiding groove 132, traction groove 133, card-in groove 134;

Sample module 21, sample holder 211, tube hole 2111, dovetail slot 2112, positioning slot 2113, push-pull handle 2114, elastic positioning sleeve 212, ring sleeve 2121, elastic positioning arm 2122, dovetail 2123, positioning base 213, positioning slide 2131;

a suction head supply module 22, a suction head tray 221, and a mounting hole 222;

A buffer liquid supply module 23 and a liquid supply box 231;

Mixing module 24, mixing tray 241, mixing tank 242;

Pipetting mechanism 25, pipettor 251, X-axis slide rail 252, X-axis drive mechanism 253, arch 254, Y-axis slide rail 255, Y-axis drive mechanism 256, Z-axis slide rail 257, Z-axis drive mechanism 258;

A support plate 26 and a push-pull plate 27.

The card storing mechanism 31, the accommodating cavity 311, the embedded groove 3111, the cartridge holder box 312, the accommodating cavity 3121, the card outlet port 3122, the elastic sheet 3123, the guide rib 3124, the box body 3125, the side plate 3126 and the drying block 313;

A discharging traversing mechanism 32, a traversing slide rail 321 and a traversing driving mechanism 322;

The material stirring mechanism 33, the material stirring seat 331, the material receiving seat 332, the material receiving groove 3321, the guide piece 3322, the material stirring slide rail 333, the material stirring pin 334, the material stirring driving mechanism 335, the material stirring motor 3351, the material stirring belt wheel 3352 and the material stirring belt 3353.

Incubation module 41, incubation seat 411, incubation cavity 412, elastic press piece 413;

A ferry mechanism 42, a ferry displacement assembly 421, a ferry slide 4211, a ferry drive assembly 4212; traction assembly 422, traction seat 4221, ferry cavity 4222, traction slide 4223, traction pin 4224, traction drive assembly 4225; card feeding assembly 423, card feeding slide rail 4231, card changing seat 4232, pushing part 4233 and card feeding driving assembly 4234;

The detecting mechanism 43, the detecting seat 431, the detecting cavity 432, the optical detecting module 433 and the receiving hopper 434.

Detailed Description

The present utility model will be described in detail with reference to specific embodiments and drawings.

The full-automatic immunofluorescence analyzer of this embodiment, as shown in fig. 1 to 13, includes a frame 1, and a sample buffer and homogenization mechanism, a reagent card discharge mechanism and a fluorescence analysis mechanism which are disposed on the frame 1, and each link is described in detail below.

The sample buffer and liquid homogenizing mechanism comprises a sample module 21, a suction head supply module 22, a buffer liquid supply module 23, a mixing module 24 and a liquid transferring mechanism 25 which are arranged on the frame 1.

The sample module 21 includes a plurality of rows of sample frames 211, the number of this example is five, and every row of sample frames 211 is provided with a plurality of tube holes 2111 that are used for placing test tubes, and tube holes 2111 are inserted with elastic positioning sleeves 212 that elastically support the outer wall of test tubes, and elastic positioning sleeves 212 include ring sleeve 2121 and more than two elastic positioning arms 2122 that extend towards tube holes 2111, and elastic positioning arms 2122 are fixed in ring sleeve 2121, and ring sleeve 2121 cooperates with the inner wall of tube holes 2111, and the test tubes placed on sample frames 211 are supported by elastic positioning arms 2122, can avoid test tube rocking, and the accurate sample of pipetting mechanism 25 of being convenient for. Specifically, the inner wall of the tube hole 2111 of the sample holder 211 is provided with a longitudinally arranged dovetail groove 2112, the elastic positioning sleeve 212 is provided with a dovetail 2123, the dovetail 2123 and the dovetail groove 2112 are in sliding fit with each other, and the insertion of the elastic positioning sleeve 212 is more accurate and convenient. Specifically, the sample module 21 further includes a positioning base 213, a plurality of positioning slide rails 2131 are disposed on top of the positioning base 213, and a positioning groove 2113 is disposed at the bottom of the sample rack 211, where the positioning groove 2113 and the positioning slide rails 2131 are slidably matched with each other. The front end of the sample rack 211 is provided with an I-shaped push-pull handle 2114, which is convenient for medical staff or peripheral mechanical arms to take and put the sample rack 211.

In this embodiment, the tip supply module 22 includes a tip tray 221, where the tip tray 221 is provided with a plurality of placement holes 222 into which the tips are inserted, and a row of tips, actually a plurality of rows, are shown in the figure, and the upper ends of the tips are located above the tip tray 221 for the pipetting mechanism 25 to take away. The buffer liquid supply module 23 includes a liquid supply box 231 with an upward opening, so that the pipetting mechanism 25 sucks the buffer liquid into the mixing module 24. The mixing module 24 comprises a liquid mixing disc 241, wherein the liquid mixing disc 241 is provided with a plurality of mixing grooves 242 with upward openings, and each mixing groove 242 is elliptical; the rack 1 is also provided with a sample discharging station 11, the sample discharging station 11 is provided with a reagent card, and the pipetting mechanism 25 sucks the blood sample and the buffer solution to the mixing tank 242 for a preset time, and then sucks the mixed solution to the reagent card for detection.

The pipetting mechanism 25 includes a pipetting device 251 and a pipetting drive module, which can drive the pipetting device 251 to move between the sample module 21, the pipette tip supply module 22, the buffer supply module 23, the mixing module 24 and the sample loading station 11. The pipetting drive module comprises an X-axis slide rail 252, an X-axis drive mechanism 253, an arch frame 254, a Y-axis slide rail 255, a Y-axis drive mechanism 256, a Z-axis slide rail 257 and a Z-axis drive mechanism 258, wherein the X-axis slide rail 252 is fixed on the frame 1, the arch frame 254 is slidably arranged on the X-axis slide rail 252, and the X-axis drive mechanism 253 drives the arch frame 254 to move along the X-axis; the Y-axis sliding rail 255 is fixed on the arch frame 254, the Z-axis sliding rail 257 is slidably mounted on the Y-axis sliding rail 255, and the Y-axis driving mechanism 256 drives the Z-axis sliding rail 257 to move along the Y-axis; the pipette 251 is slidably mounted on a Z-axis slide 257, and a Z-axis drive mechanism 258 drives the pipette 251 to move along the Z-axis.

Specifically, the X-axis driving mechanism 253, the Y-axis driving mechanism 256 and the Z-axis driving mechanism 258 each include a pipetting motor, a transmission wheel and a belt, the pipetting motor drives the belt to move through the transmission wheel, for example, the gantry 254, the Z-axis sliding rail 257 and the pipettor 251 are respectively fixed on the corresponding belts, and the belts can drive the belts to move linearly when moving.

In this embodiment, the support plate 26 is further disposed on the frame, and the sample module 21, the suction head supply module 22, the buffer liquid supply module 23 and the mixing module 24 are all disposed on the support plate 26, and during manufacturing, these modules are positioned on the support plate 26, and then the support plate 26 is fixed on the frame, so that the whole module is convenient to install.

Specifically, a push-pull plate 27 is slidably arranged on the support plate 26, and the suction head supply die and mixing die set 24 is placed on the push-pull plate 27; the push-pull plate 27 is provided with a handle to facilitate the separate insertion or withdrawal of the two modules.

Specifically, the bottom of frame still is equipped with suction head recovery chamber 12, and suction head recovery chamber 12 has placed the recovery box that does not show in the figure, and the frame is opened there is the recovery hole that pipetting mechanism 25 can align, and recovery hole aligns the recovery box, and the suction head is disposable, and after the sample next time, the suction head can be abandoned, falls to in the recovery box from recovery hole.

The reagent card discharging mechanism is used for conveying reagent cards to the sample discharging stations one by one and comprises a card storing mechanism 31, a discharging transverse moving mechanism 32, a material shifting mechanism 33 and the sample discharging stations 11 which are arranged on the frame 1.

The card storing mechanism 31 is provided with a plurality of accommodating cavities 311, the bottom of the accommodating cavities 311 is provided with a plurality of embedded grooves 3111 arranged in parallel, and the bottoms of the plurality of cartridge boxes 312 are inserted into the accommodating cavities 311 with the embedded grooves 3111 as guiding. Five cartridge bodies 312 are arranged side by side in the figure, and may be changed to other numbers as needed. The inside of cartridge clip box body 312 is provided with the accomodate chamber 3121 that supplies reagent card 13 range upon range of to place, and the front side portion that corresponds bottom reagent card 13 of cartridge clip box body 312 is equipped with out bayonet socket 3122, and play bayonet socket 3122 is provided with elastic piece 3123, and elastic piece 3123 blocks out bayonet socket 3122 and avoids reagent card 13 to fall out at will or the too big dust of opening gets into, can jack up elastic piece 3123 when the bottom reagent card 13 of cartridge clip box body 312 is pushed out the ejection of compact, and the upper end card of elastic piece 3123 is on cartridge clip box body 312 lateral wall, and the lower extreme can atress deformation. Specifically, the side portion of the reagent card 13 is provided with a guiding groove 132, the inner wall of the cartridge holder box 312 is provided with a guiding rib 3124 longitudinally arranged, the guiding rib 3124 is in sliding fit with the guiding groove 132, the deflection of the reagent card 13 is avoided, and the reagent card 13 is ensured to vertically descend one by one. The cartridge box body 312 is also provided with a drying block 313, the drying block 313 presses the reagent card 13 on the inner top layer, the inside of the cartridge box body 312 is kept dry and prevented from being wet, and the side part of the drying block 313 is also provided with a guide groove 132 which is matched with the guide rib 3124 on the inner wall of the cartridge box body 312 in a sliding way. Specifically, the cartridge clip case 312 includes a case body 3125 and a side plate 3126, where the side plate 3126 and the case body 3125 are detachably connected through a fastening structure, and the side plate 3126 is convenient to open and add the reagent card 13 therein after the side plate 3126 is detached.

The material stirring mechanism 33 comprises a material stirring seat 331, a material receiving seat 332 which is fixed on the material stirring seat 331 and provided with a material receiving groove 3321, a material stirring slide rail 333 which is fixed on the material stirring seat 331, a material stirring pin 334 which is slidably arranged on the material stirring slide rail 333, and a material stirring driving mechanism 335 for driving the material stirring pin 334 to move on the material stirring slide rail 333. The material stirring driving mechanism 335 comprises a material stirring motor 3351, a material stirring belt wheel 3352 and a material stirring belt 3353, the material stirring motor 3351 drives the material stirring belt 3353 to rotate through the material stirring belt wheel 3352, the material stirring belt 3353 is arranged along the length direction of the material stirring sliding rail 333, and the material stirring pin 334 is fixed on the material stirring belt 3353. Specifically, the end of the reagent card 13 is provided with a U-shaped positioning notch 131 into which a stirring pin 334 is inserted. Specifically, the notch of the receiving slot 3321 is provided with a guide piece 3322, so that the reagent card 13 can be smoothly guided and inserted.

The discharging traversing mechanism 32 comprises traversing slide rails 321 arranged below the memory card mechanism 31, the arranging direction of the traversing slide rails 321 is the same as the parallel direction of the plurality of cartridge clip boxes 312, the material shifting seat 331 is slidably matched on the traversing slide rails 321, and the material shifting slide rails 333 are arranged perpendicular to the traversing slide rails 321; the discharging traversing mechanism 32 further comprises a traversing driving mechanism 322 for driving the material shifting seat 331 to move along the traversing slide rail 321. Similarly, the traverse driving mechanism 322 and the material shifting driving mechanism 335 are similar in mode of motor driving belt wheels, and can be actually changed into a common screw-nut driving mechanism, and can also realize conventional linear motion. Specifically, the rear side portion of the cartridge body 312 corresponding to the bottom reagent card 13 is provided with a relief hole into which the material pulling pin 334 is inserted.

In operation, in the initial state, the material shifting mechanism 33 is located at the position shown in fig. 1, the material shifting pin 334 is staggered with the cartridge box body 312, so that the material shifting mechanism can retreat to a position close to the rear side of the cartridge box body 312 without interference, the transverse moving driving mechanism 322 drives the material receiving seat 332 to move leftwards to align with the outlet 3122 of one of the cartridge box bodies 312, the material shifting pin 334 of the material shifting mechanism 33 is inserted into the yielding hole of the cartridge box body 312, and the bottom reagent card 13 in the cartridge box body 312 is pushed forwards to the material receiving groove 3321 under the action of the material shifting driving mechanism 335 for the positioning notch 131 of the Ji Shiji card 13; then, the discharging traversing mechanism 32 drives the material shifting mechanism 33 to move rightwards, so that the material receiving seat 332 is longitudinally aligned with the sample discharging station 11 to receive samples dripped by the pipettor 251, and the reagent cards 13 in different cartridge boxes 312 are sequentially and circularly discharged.

The fluorescence analysis mechanism includes an incubation module 41, a ferrying mechanism 42 and a detection mechanism 43 provided on the frame 1.

The incubation module 41 comprises an incubation seat 411, the incubation seat 411 being provided with a plurality of incubation cavities 412 arranged side by side for placing the reagent cards 13 loaded with samples. After the reagent card 13 in the receiving seat 332 receives the sample, the reagent card 13 is pushed to be inserted into the incubation cavity by the stirring pin 334 under the drive of the discharging traversing mechanism 32 to align different incubation cavities 412. Specifically, the wall of the incubation cavity 412 is provided with an elastic pressing piece 413 for elastically pressing the reagent card 13 up and down, so as to avoid the reagent card 13 from being released and displaced.

The detection mechanism 43 includes a detection seat 431 and an optical detection module 433, the detection seat 431 is provided with a detection cavity 432 into which the reagent card 13 to be detected is placed, the detection cavities 432 are arranged beside the incubation cavities 412 in parallel, and the optical detection module 433 is fixed on the detection seat 431 to detect the reagent card 13 to be detected. The optical detection module 433 is an outsourcing component, which belongs to a fluorescence analysis module in the prior art, and is not described herein. As regards the transfer of the reagent card 13 from the plurality of incubation chambers 412 to the detection chamber 432, this is achieved by the ferrying mechanism 42:

The indexing mechanism 42 is used to move reagent cards 13 from one incubation cavity 412 to the detection cavity 432. Specifically, the ferrying mechanism 42 includes a ferrying displacement assembly 421, a traction assembly 422 and a card feeding assembly 423, the traction assembly 422 includes a traction base 4221, a traction slide 4223, a traction pin 4224 and a traction driving assembly 4225, the traction slide 4223 is fixed on the traction base 4221, the traction pin 4224 is slidably matched on the traction slide 4223, the traction base 4221 is provided with a ferrying cavity 4222, the traction driving assembly 4225 drives the traction pin 4224 to move on the traction slide 4223, and the end of the reagent card 13 is provided with a traction groove 133 for the traction pin 4224 to clamp.

The ferry displacement assembly 421 includes a ferry slide 4211 and a ferry drive assembly 4212, the ferry slide 4211 being disposed in a side-by-side orientation along the plurality of incubation cavities 412, the traction seat 4221 being slidably mounted on the ferry slide 4211, the ferry drive assembly 4212 driving the traction seat 4221 to move along the ferry slide 4211 such that the ferry cavities 4222 are aligned with different incubation cavities 412 and detection cavities 432.

Card feed assembly 423 is used to move reagent card 13 in transition chamber 4222 to detection chamber 432. Specifically, the card feeding assembly 423 includes a card feeding rail 4231, a card changing seat 4232, and a card feeding driving assembly 4234, wherein the card changing seat 4232 is slidably engaged with the card feeding rail 4231, and the card feeding driving assembly 4234 drives the card changing seat 4232 to slide along the card feeding rail 4231. The card changing base 4232 is provided with a pushing part 4233 capable of unidirectional elastic swinging, the pushing part 4233 is hinged on the card changing base 4232, the hinge shaft is provided with a button force spring, the reagent card 13 is provided with a card feeding groove 134 into which the pushing part 4233 is inserted, the pushing part 4233 can be inserted into the card feeding groove 134 forward to push the reagent card 13, and backward swinging can be separated from the card feeding groove 134.

As can be seen from the figure, the guiding driving assembly 4212, the traction driving assembly 4225 and the card feeding driving assembly 4234 are all motors driving pulleys, the pulleys driving belts to rotate, and the traction pins 4224, the card changing seats 4232 and the traction seats 4221 are fixed on the corresponding belts. Of course, in practice, other forms of linear drive mechanisms, such as a screw-nut mechanism, may be substituted.

Specifically, the detection mechanism 43 is provided with a receiving hopper 434 at a side thereof, and the reagent card 13 coming out of the detection chamber 432 falls down from the inclined receiving hopper 434.

During operation, the reagent card 13 loaded with the sample is kept stand in the incubation cavities 412 for reaction preset time, the traction driving assembly 4212 drives the traction seat 4221 to move along the traction sliding rail 4211 so as to align the traction cavity 4222 with one of the incubation cavities 412, the traction driving assembly 4225 drives the traction pin 4224 to be embedded into the traction groove 133, and the traction groove 133 is similar to an L shape, so that the traction driving assembly 4212 needs to slightly transversely move, and the reagent card 13 can be mutually clamped in the in-out direction of the reagent card 13 so as to move the reagent card 13 in the incubation cavity 412 into the traction cavity 4222; then, the traction driving assembly 4212 drives the traction seat 4221 to move along the traction sliding rail 4211 so as to align the traction cavity 4222 with the detection cavity 432, the traction driving assembly 4225 drives the traction pin 4224 to push the reagent card 13 to partially enter the detection cavity 432, then the card feeding driving assembly 4234 drives the pushing part 4233 to move so as to move the reagent card 13 into place, and the pushing part 4233 is retracted and reset, and then the reagent card can be detected. The reagent cards 13 in the incubation cavities 412 are automatically fed and discharged for detection.

In the description of the present utility model, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "secured" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Standard parts used in the utility model can be purchased from the market, special-shaped parts can be customized according to the description of the specification and the drawings, the specific connection modes of all parts adopt conventional means such as mature bolts, rivets and welding in the prior art, the machinery, the parts and the equipment adopt conventional models in the prior art, and the circuit connection adopts conventional connection modes in the prior art, so that the details are not described.

Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present utility model, and not for limiting the scope of the present utility model, and although the present utility model has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made to the technical solution of the present utility model without departing from the spirit and scope of the technical solution of the present utility model.

Claims (10)

1. Full-automatic immunofluorescence analyzer, characterized by: the device comprises a frame, a sample buffer and liquid homogenizing mechanism, a reagent card discharging mechanism and a fluorescence analysis mechanism, wherein the sample buffer and liquid homogenizing mechanism, the reagent card discharging mechanism and the fluorescence analysis mechanism are arranged on the frame;

The sample buffer and liquid homogenizing mechanism comprises a sample module, a suction head supply module, a buffer liquid supply module, a mixing module and a liquid transferring mechanism; the sample module comprises a plurality of rows of sample frames, each row of sample frames is provided with a plurality of tube holes for placing test tubes, and the tube holes are inserted with elastic positioning sleeves which elastically prop against the outer walls of the test tubes; the suction head supply module comprises a suction head disc, and a plurality of placing holes for the suction heads to be inserted are formed in the suction head disc; the buffer liquid supply module comprises a liquid supply box with an upward opening; the mixing module comprises a liquid mixing disc, wherein the liquid mixing disc is provided with a plurality of mixing grooves with upward openings; the frame is also provided with a sample feeding station; the pipetting mechanism comprises a pipetting device and a pipetting drive module, and the pipetting drive module can drive the pipetting device to move among the sample module, the suction head supply module, the buffer liquid supply module, the mixing module and the sample loading station;

The reagent card discharging mechanism comprises a card storing mechanism, a discharging transverse moving mechanism, a material shifting mechanism and a sample discharging station, wherein the card storing mechanism is provided with a plurality of accommodating cavities for accommodating cartridge clip box bodies, the inside of the cartridge clip box bodies is provided with accommodating cavities for stacking and placing reagent cards, and the front side parts of the cartridge clip box bodies, which correspond to the bottom reagent cards, are provided with outlet openings; the material stirring mechanism comprises a material stirring seat, a material receiving seat which is fixed on the material stirring seat and provided with a material receiving groove, a material stirring sliding rail which is fixed on the material stirring seat, a material stirring pin which is slidably arranged on the material stirring sliding rail, and a material stirring driving mechanism for driving the material stirring pin to move on the material stirring sliding rail, and is used for pushing the bottom reagent card in the cartridge case forwards into the material receiving groove; the discharging transverse moving mechanism comprises a transverse moving slide rail arranged below the card storage mechanism, the material shifting seat is slidably matched on the transverse moving slide rail, and the material shifting slide rail is vertically arranged with the transverse moving slide rail; the discharging transverse moving mechanism further comprises a transverse moving driving mechanism for driving the material shifting seat to move along the transverse moving sliding rail so as to drive the material receiving seat to move between the plurality of cartridge box bodies and the sample loading station;

The fluorescence analysis mechanism comprises an incubation module, a transition mechanism and a detection mechanism, wherein the incubation module comprises an incubation seat, and the incubation seat is provided with a plurality of incubation cavities which are used for placing reagent cards loaded with samples and are arranged in parallel; the detection mechanism comprises a detection seat and an optical detection module, the detection seat is provided with a detection cavity for placing the reagent card to be detected, and the optical detection module is fixed on the detection seat to detect the reagent card to be detected; the transfer mechanism is used for moving the reagent cards in the plurality of incubation cavities to the detection cavity one by one.

2. The fully automatic immunofluorescence analyzer of claim 1, wherein: the sample module further comprises a positioning base, the top of the positioning base is provided with a plurality of positioning sliding rails, the bottom of the sample frame is provided with positioning grooves, and the positioning grooves are in sliding fit with the positioning sliding rails; the inner wall of the tube hole of the sample frame is provided with a longitudinally arranged dovetail groove, the elastic positioning sleeve is provided with a dovetail, and the dovetail groove are mutually matched in a sliding way; the elastic positioning sleeve comprises a ring sleeve and an elastic positioning arm extending towards the inside of the pipe hole, the elastic positioning arm is fixed on the ring sleeve, and the ring sleeve is matched with the inner wall of the pipe hole.

3. The fully automatic immunofluorescence analyzer of claim 1, wherein: the outlet of the cartridge clip box body is provided with an elastic sheet, the elastic sheet blocks the outlet, and the bottom reagent card of the cartridge clip box body is pushed by the material poking pin to jack the elastic sheet; the cartridge clip box body is also internally provided with a drying block body, and the drying block body presses the reagent card on the inner top layer of the cartridge clip box body.

4. The fully automatic immunofluorescence analyzer of claim 1, wherein: the traction mechanism comprises a traction shifting assembly, a traction assembly and a clamping assembly, the traction assembly comprises a traction seat, a traction sliding rail, a traction pin and a traction driving assembly, the traction sliding rail is fixed on the traction seat, the traction pin is slidably matched on the traction sliding rail, the traction seat is provided with a traction cavity, and the traction driving assembly drives the traction pin to move on the traction sliding rail so as to clamp and move the reagent in the incubation cavity into the traction cavity;

The ferry displacement assembly comprises a ferry sliding rail and a ferry driving assembly, the ferry sliding rail is arranged along the parallel directions of the plurality of incubation cavities, the traction seat is slidably arranged on the ferry sliding rail, and the ferry driving assembly drives the traction seat to move along the ferry sliding rail, so that the ferry cavities are aligned with different incubation cavities and detection cavities;

The card feeding assembly is used for moving the reagent card in the ferrying cavity to the detection cavity.

5. The fully automatic immunofluorescence analyzer of claim 4, wherein: the card feeding assembly comprises a card feeding slide rail, a card changing seat and a card feeding driving assembly, wherein the card changing seat is slidably matched with the card feeding slide rail, and the card feeding driving assembly drives the card changing seat to slide along the card feeding slide rail.

6. The fully automatic immunofluorescence analyzer of claim 5, wherein: the card changing seat is provided with a pushing part capable of unidirectional elastic swinging, the reagent card is provided with a card feeding groove for embedding the pushing part, the pushing part can be embedded into the card feeding groove forward to push the reagent card, and backward swinging can be separated from the card feeding groove.

7. The fully automatic immunofluorescence analyzer of claim 1, wherein: the end part of the reagent card is provided with a traction groove for clamping a traction pin; the end of the reagent card is provided with a positioning notch for embedding the stirring pin.

8. The fully automatic immunofluorescence analyzer of claim 1, wherein: the wall of the incubation cavity is provided with an elastic pressing piece.

9. The fully automatic immunofluorescence analyzer of claim 1, wherein: the cartridge clip box body is also internally provided with a drying block body, and the drying block body presses the reagent card on the inner top layer of the cartridge clip box body.

10. The fully automatic immunofluorescence analyzer of claim 1, wherein: the pipetting drive module comprises an X-axis slide rail, an X-axis drive mechanism, an arch portal frame, a Y-axis slide rail, a Y-axis drive mechanism, a Z-axis slide rail and a Z-axis drive mechanism, wherein the X-axis slide rail is fixed on the frame, the arch portal frame is slidably arranged on the X-axis slide rail, and the X-axis drive mechanism drives the arch portal frame to move along the X axis; the Y-axis sliding rail is fixed on the arch portal, the Z-axis sliding rail is slidably arranged on the Y-axis sliding rail, and the Y-axis driving mechanism drives the Z-axis sliding rail to move along the Y axis; the liquid transfer device is slidably arranged on the Z-axis sliding rail, and the Z-axis driving mechanism drives the liquid transfer device to move along the Z axis; the X-axis driving mechanism, the Y-axis driving mechanism and the Z-axis driving mechanism all comprise a pipetting motor, a transmission wheel and a belt, and the pipetting motor drives the belt to move through the transmission wheel.