CN116374355A - Automatic feeding equipment and method for reagent card strips - Google Patents

Abstract

The invention relates to automatic feeding equipment and method for reagent card strips, and belongs to the technical field of automatic experimental equipment. Reagent card strip automatic feeding equipment includes: the storage mechanism comprises a storage shell, and a drawer, a card pushing mechanism and a locking mechanism which are arranged in the storage shell; the receiving mechanism comprises a lifting mechanism and a receiving assembly; the conveying mechanism comprises a conveying seat; the film puncturing mechanism comprises a puncturing assembly and a film puncturing driving device; and the storage mechanism, the access mechanism, the conveying mechanism and the film puncturing mechanism are all arranged on the frame. Above-mentioned reagent card strip automatic feeding equipment to storage mechanism, access mechanism, conveying mechanism and thorn membrane mechanism's cooperation has realized whole automated solution, has solved artifical material loading operation complicated, problem that the human input cost is high, and degree of automation is high, and above-mentioned equipment has compact structure, advantage small.

Description

Automatic feeding equipment and method for reagent card strips

Technical Field

The invention relates to the technical field of automatic experimental equipment, in particular to automatic feeding equipment and method for reagent card strips.

Background

With rapid development of science and technology, nucleic acid detection technology has been widely used in the biomedical field. With the increasing demand for nucleic acid detection, the time of the experimental period is further shortened, and the requirement for automation of nucleic acid detection instruments is increasing.

Currently, the existing nucleic acid extraction technology generally requires four steps of cleavage, binding, washing, and elution. The extraction method conventionally adopted is a centrifugal column method or a magnetic bead method, and the extraction implementation route is divided into microfluidic cartridge (chip) extraction or deep-hole plate extraction and the like.

In a detection laboratory, a conventional nucleic acid extraction method requires a professional to operate various instruments and equipment to complete a plurality of steps such as sample pretreatment, nucleic acid extraction and purification, detection system preparation, amplification, detection and the like. In the whole process, the process is complex, the operation is complex, a large amount of manpower is required to be input, and the fatigue efficiency is easy to reduce in the laboratory operation environment for a long time by workers, and the workers are likely to contact waste liquid in waste consumables, so that pollution is caused. Therefore, the demands of people for instruments with high automation degree and stable performance, which can reduce manual operation, are more and more urgent.

However, for the sample loading step of the reagent card strip, the conventional automatic nucleic acid extraction equipment often needs to manually remove the package and the sealing film of the reagent card strip and then manually load the sample, so that the degree of automation is low; or the loading equipment with high automation degree has the problems of complex structure, large volume and the like.

Disclosure of Invention

Based on this, it is necessary to provide an automatic feeding device for reagent card strips, by which full-automatic feeding of reagent card strips can be realized, and the device has the advantage of compact structure.

Reagent card strip automatic feeding equipment includes:

the storage mechanism comprises a storage shell, and a drawer, a card pushing mechanism and a locking mechanism which are arranged in the storage shell, wherein a containing cavity for containing the reagent card strip is arranged in the drawer, the card pushing mechanism comprises a card pushing driving device, a card pushing assembly and a card pushing guide rail, and the card pushing assembly moves along the card pushing guide rail under the driving of the card pushing driving device so as to push the reagent card strip placed in the containing cavity to the edge of the drawer; the drawer can be respectively positioned at a closed state position and an extracted state position along the sliding rail in the storage shell in a pulling manner, the outer wall of the drawer is provided with a slot piece, a slot hole is formed in the slot piece, the locking mechanism comprises a bolt and a bolt driving device, when the drawer is positioned at the closed state position, the position of the bolt corresponds to the position of the slot hole, and the bolt can be driven by the bolt driving device to be inserted into or withdrawn from the slot hole;

the collecting mechanism comprises a lifting mechanism and a collecting component, the collecting component can be driven by the lifting mechanism to do lifting motion and is provided with a card collecting state position and a card feeding state position, the card collecting state position is positioned below the card feeding state position, when the collecting component is positioned at the card collecting state position, the collecting component corresponds to the edge position of the drawer, the collecting component comprises a collecting base plate, a telescopic driving device, a lifting component and a collecting plate which are sequentially connected, the collecting component is connected with the lifting mechanism through the collecting base plate, the telescopic component is driven by the telescopic driving device to drive the collecting plate to do telescopic motion, and a collecting piece matched with a reagent card strip is arranged on the collecting plate;

the conveying mechanism comprises a conveying seat, a placing table for placing reagent card strips is arranged on the conveying seat, and the conveying seat corresponds to a card feeding state position of the receiving component; and

the puncturing mechanism comprises a puncturing assembly and a puncturing film driving device, wherein a puncturing head is arranged on the puncturing assembly, and can downwards puncture and reset the reagent card strip on the opposite table under the driving of the puncturing film driving device.

Above-mentioned reagent card strip automatic feeding equipment to storage mechanism, connect and get mechanism, conveying mechanism and thorn membrane mechanism's cooperation has realized automatic whole solution, can utilize storage mechanism to push away the card automatically, makes reagent card strip by one by the propelling movement to connect and gets the mechanism and get the card position, later utilize to connect and get the subassembly to stretch out and get the board and get the card after, upwards rise reagent card strip through elevating system, puncture after reaching thorn membrane mechanism working position, rely on conveying mechanism to pass reagent card strip to other stations again. The automatic feeding device solves the problems of complicated manual feeding operation and high labor input cost, and has the advantages of high automation degree and compact structure.

Meanwhile, the equipment adopts the repeatedly-usable puncture head to puncture, so that on one hand, the equipment structure is simplified, the time for taking and losing the disposable puncture head is reduced, the space for storing the disposable puncture head is reduced, and the cost is saved. And controlling the drawer state by the locking mechanism, so as to avoid mechanical faults caused by the drawer being pulled out in the working state.

In one embodiment, the locking mechanism further comprises a locking bracket and an elastic piece, the bolt is slidably mounted on the locking bracket, one end of the elastic piece is propped against the bolt, the other end of the elastic piece is fixed, the bolt has a trend of being inserted into the slot hole, the bolt driving device is an electromagnet and is fixed on the locking bracket, the output end of the electromagnet is connected with the bolt, and the bolt is driven to withdraw from the slot against the elastic force of the elastic piece.

In one embodiment, the bolt head is provided with an arc-shaped guide surface, and the slot piece is provided with a guide slope;

the output end of the electromagnet is connected with the bolt through a power connecting rod;

the bolt is arranged on the locking bracket through a sliding bearing.

Through the cooperation of above-mentioned mechanism, locking mechanism is in the state of stretching out always under the effect of elastic component (such as spring) when outage, and when the drawer pushed in the in-process, the arc guide surface (such as cylinder head) of bolt head was pushed up along the direction slope of slot spare periphery, until the drawer pushes into the closed state position, and the bolt corresponds with slot hole position, and the bolt is inserted in the slot hole in back, and the drawer is locked. The electromagnet needs to be started to pull out the drawer. And a spring is arranged on the bolt, so that the purpose of the spring is to prevent the push-pull rod of the electromagnet from being forced to return by the elasticity provided by the spring when the push-pull rod of the electromagnet cannot return to the original position due to residual magnetism.

The electromagnet is in a power-off stretching type, the plug pin is driven by the electromagnet through the power connecting rod, the push-pull force of the drawer is prevented from directly acting on the push-pull rod of the electromagnet to cause the bending of the push-pull rod, and the service life of the electromagnet is prolonged. The bolt is arranged on the locking bracket through the sliding bearing, so that the sliding guide of the bolt is ensured, and the push-pull force of the drawer born by the bolt can be transmitted to the locking rack.

In one embodiment, the card pushing assembly comprises a card pushing screw, a card pushing nut and a card pushing piece, the card pushing driving device is a motor, the output end of the motor is connected with the card pushing screw, the card pushing nut is sleeved on the card pushing screw and connected with the card pushing piece, and the card pushing piece is connected with the card pushing guide rail in a sliding manner;

and a positioning piece used for limiting the position of the reagent card strip is also arranged in the drawer.

Through the cooperation of the locating piece in the drawer and the clamping piece on the outer wall of the clamping box, the clamping box can be limited to be placed at the front, back, left and right positions in the drawer, and the accurate and stable clamping pushing action of the clamping pushing mechanism is facilitated.

In one embodiment, the access member is an access strip extending from the access plate in the direction of the reagent card strip;

the telescopic driving device is a linear motor, and the telescopic component is a linear bearing;

the access assembly further comprises a telescopic optocoupler for identifying the position of the access plate;

the lifting mechanism comprises a lifting optocoupler, a lifting driving device and a lifting driving belt, wherein the lifting optocoupler is used for identifying the position of the receiving component, the lifting driving device drives the lifting driving belt to move, and the receiving component is fixed on the lifting driving belt.

The access plate and the extended access member together form a U-shaped opening into which the cartridge package housing can be accessed while avoiding. When the access plate is lifted, the reagent card strip can be lifted out of the card box.

In one embodiment, the receiving member is provided with a limiting groove for limiting the position of the reagent card strip. The limiting groove is used for limiting the movement of the reagent card strip on the access plate and preventing the reagent card strip from falling off in the transportation process.

In one embodiment, the conveying mechanism further comprises a conveying driving device, a conveying piece and a conveying guide rail, the conveying piece moves along the conveying guide rail under the driving of the conveying driving device, and the conveying seat is fixed on the conveying piece.

In one embodiment, the conveying seat is driven by the conveying piece to move along the conveying guide rail to a puncture position corresponding to the film puncturing mechanism, a liquid moving level corresponding to the liquid moving device and a waiting position waiting for the next process;

the conveying seat further comprises a liquid level shifting sensor and a waiting position sensor, when the conveying seat is positioned at the liquid level shifting position, the liquid level shifting sensor is triggered to generate signals, and when the conveying seat is positioned at the waiting position, the waiting position sensor is triggered to generate signals.

In one embodiment, the film puncturing mechanism further comprises a film puncturing fixing frame, a film puncturing screw rod and a film puncturing sliding block, wherein the film puncturing driving device is arranged on the film puncturing fixing frame, the film puncturing screw rod is connected with the output end of the film puncturing driving device, the film puncturing sliding block is sleeved on the film puncturing screw rod, and the puncturing assembly is fixed on the film puncturing sliding block;

the puncture head is of a solid triangular prismatic structure.

The puncture head is in the structure, which is favorable for puncturing the packaging film and facilitating the puncture head to separate from the reagent card strip.

In one embodiment, the film punching mechanism further comprises a pre-pressing module, the pre-pressing module comprises a pre-pressing block, a pre-pressing elastic piece, a pressing block guide shaft, a pre-pressing stop block and a pre-pressing support, a guide hole is formed in the pre-pressing support, the pre-pressing guide shaft penetrates through the guide hole, one end of the pre-pressing guide shaft, which is located above the pre-pressing support, is the top end, the other end of the pre-pressing guide shaft, which is located below the pre-pressing support, is the bottom end, the maximum radial distance of the pre-pressing stop block is greater than the diameter of the guide hole and is arranged at the top end of the pre-pressing guide shaft, the pre-pressing block is arranged at the bottom end of the pre-pressing guide shaft, one end of the pre-pressing elastic piece abuts against the pre-pressing support, and the other end of the pre-pressing block abuts against the pre-pressing block, so that the pre-pressing block has a downward movement trend.

The prepressing modules can be divided into two groups and are respectively arranged at the left end and the right end of the reagent clamping strip. When puncturing, the pre-pressing module firstly contacts the reagent clamping strip and applies certain pressure to prevent the reagent clamping strip from moving; the puncture head then punctures the encapsulation film. After puncturing, the puncture head rises, and after completely leaving the non-contact reagent card strip, the pre-pressing module starts to break away from contact with the reagent card strip. This prevents the piercing tip from bringing the reagent strip together when it is disengaged.

In one embodiment, the pushing card assembly, the lifting mechanism, the receiving assembly, the conveying seat and the puncture assembly are two sets to cooperate with the simultaneous processing of two reagent card strips;

and/or

The number of drawers is two, and the drawers are placed in the storage shell in an up-down overlapping mode.

Through increasing the quantity of pushing away card subassembly, elevating system, access subassembly, delivery seat and puncture subassembly, allow multichannel simultaneous operation, improve automatic feeding efficiency. The large volume of the drawers is utilized, so that the quantity of reagent card strips can be increased, the time interval for manually replenishing the reagent card strips is increased, the independent operation and no mutual interference are facilitated in the working process, when one of the two drawers is out of feed, the other drawer is pulled out to replenish the feed, the continuous working of the other drawer is not influenced, and the problem that the equipment must be stopped to replenish the feed when the materials are used up is solved; or one of the channels fails, the other set of mechanisms can continue to operate.

The invention also discloses a reagent card strip assembly, which comprises a reagent card strip matched with the automatic feeding equipment, wherein the top of the reagent card strip is provided with a packaging film, and two ends of the reagent card strip are provided with clamping pieces matched with the receiving pieces and the placing table.

In one embodiment, the reagent card strip further comprises a card box, a plurality of reagent card strips are arranged in the card box, the clamping pieces at two ends of the reagent card strip extend to the outer edge of the top of the card box, and the clamping pieces are in a hook shape with downward openings;

the hole site includes: tip and reagent sites;

the outer wall of the card box is also provided with a clamping piece, and the clamping piece is correspondingly matched with a positioning piece which is arranged in the storage shell and used for limiting the position of the card box.

The invention also discloses a method for automatically feeding the reagent card strip, which adopts the automatic feeding equipment for the reagent card strip and comprises the following steps:

pushing cards: placing the reagent card strip assembly in a containing cavity of the drawer, and pushing the reagent card strip to the edge of the drawer under the action of the card pushing assembly;

lifting: the lifting mechanism drives the receiving component to descend to a preset position, the receiving plate stretches out to the lower side of the reagent clamping strip under the drive of the telescopic component, the receiving piece on the receiving plate is matched with the clamping piece of the reagent clamping strip, the lifting mechanism drives the receiving component to ascend, and the receiving piece lifts the reagent clamping strip to the preset position;

puncturing: the puncturing film driving device drives the puncturing head to puncture downwards, punctures the packaging film of the reagent card strip, and resets the puncturing head.

Compared with the prior art, the invention has the following beneficial effects:

according to the automatic feeding equipment for the reagent card strips, disclosed by the invention, the storage mechanism, the access mechanism, the conveying mechanism and the film puncturing mechanism are matched, so that an automatic integral solution is realized, the storage mechanism can be utilized to automatically push the cards, the reagent card strips are pushed to the card taking position of the access mechanism one by one, then the access assembly is utilized to extend out of the access plate to take the cards, the reagent card strips are lifted upwards through the lifting mechanism, the puncturing is performed after the reagent card strips reach the working position of the film puncturing mechanism, and the reagent card strips are transferred to other stations by virtue of the conveying mechanism. The automatic feeding device solves the problems of complicated manual feeding operation and high labor input cost, and has the advantages of high automation degree and compact structure.

Drawings

FIG. 1 is a schematic diagram of an automatic reagent card strip feeding device;

FIG. 2 is an exploded view of the mechanisms of FIG. 1;

FIG. 3 is a schematic diagram of a reagent card strip assembly;

FIG. 4 is a side view of FIG. 3;

FIG. 5 is a schematic illustration of a reagent card strip configuration;

FIG. 6 is a side view of FIG. 5;

FIG. 7 is a schematic diagram of a storage mechanism;

FIG. 8 is a side perspective view of a storage mechanism drawer closed;

FIG. 9 is a schematic illustration of the installation of reagent strips in a drawer;

FIG. 10 is a schematic illustration of a card pushing mechanism;

FIG. 11 is a schematic view of the drawer and locking mechanism engaged;

FIG. 12 is a schematic view of a locking mechanism;

FIG. 13 is an exploded view of FIG. 12;

FIG. 14 is a schematic view of a take-up mechanism;

FIG. 15 is a schematic view of a pick-up assembly;

FIG. 16 is a schematic view of the structure of FIG. 15 from another perspective;

FIG. 17 is a top view of the access panel;

FIG. 18 is a schematic view of a pick-up plate structure;

FIG. 19 is a schematic view of a pick-up plate in a pick-up state;

FIG. 20 is a schematic view of a conveying mechanism;

FIG. 21 is a schematic illustration of a film puncturing mechanism;

FIG. 22 is a side perspective view of the film puncturing mechanism;

FIG. 23 is a schematic diagram of a pre-compression module configuration;

FIG. 24 is a schematic view of a lifting mechanism in a lifting position;

fig. 25 is a schematic view of a conveying position of the conveying mechanism.

Wherein:

100. a storage mechanism; 110. a storage housing; 120. a drawer; 121. a socket member; 1211. a slot hole; 1212. a guide ramp; 123. a positioning piece; 130. a card pushing mechanism; 131. a card pushing driving device; 132. a card pushing component; 1321. pushing and clamping the screw rod; 1322. pushing and clamping the nut; 1323. the pushing clamping piece; 133. a card pushing guide rail; 140. a locking mechanism; 141. a plug pin; 1411. an arc-shaped guide surface; 142. a latch driving device; 143. locking the bracket; 144. an elastic member; 145. a sliding bearing; 1146. a power connecting rod;

200. a receiving mechanism; 210. a lifting mechanism; 211. lifting an optocoupler; 213. lifting the transmission belt; 220. a receiving assembly; 221. receiving a substrate; 222. a telescopic driving device; 223. a lifting and shrinking assembly; 224. a receiving plate; 2241. a receiving member; 2241a, limit groove; 225. a telescopic optocoupler;

300. a conveying mechanism; 310. a conveying seat; 311. a placement table; 312. a liquid level sensor; 313. a waiting position sensor; 320. a conveying driving device; 330. a conveying member; 340. a conveying guide rail;

400. a film puncturing mechanism; 410. a puncture assembly; 411. a puncture head; 420. a film puncturing driving device; 430. a thorn film fixing frame; 440. film punching screw rods; 450. film punching sliding blocks; 460. a pre-pressing module; 461. pre-pressing blocks; 462. pre-pressing the elastic piece; 463. a briquetting guide shaft; 464. pre-pressing the stop block; 465. pre-pressing a bracket;

500. a frame;

600. a reagent card strip assembly; 610. a reagent card strip; 6111. a suction head hole site; 6112. reagent hole sites; 612. packaging the film; 613. a clamping piece; 620. a cartridge; 622. a clamping piece;

700. and a pipetting mechanism.

Detailed Description

In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

Example 1

An automatic reagent card strip feeding device, as shown in fig. 1-2, comprises: the automatic reagent card strip feeding device of the present embodiment is used in combination with the reagent card strip assembly 600 shown in fig. 3, and the rack is used for providing mounting support.

As shown in fig. 3-4, the reagent card strip assembly 600 includes a reagent card strip 610 and a cartridge 620. The top of the reagent card strip 610 is provided with a packaging film 612, and two ends of the reagent card strip 610 are provided with a clamping piece 613 matching with a receiving piece 2241 in the receiving mechanism 200 and a placing table 311 in the conveying mechanism, wherein the clamping piece 613 is in a hook shape with a downward opening, as shown in fig. 5-6.

The plurality of reagent card strips 610 are arranged in parallel in the card box 620, the clamping pieces 613 at two ends of the reagent card strips 610 extend to the outer edge of the top of the card box 620, and the card box 620 is also provided with a packaging cover to ensure that the reagent card strips 610 are clean and not polluted in the production, storage and other links which do not need to be used.

In this embodiment, the hole site includes: a tip well 6111 and a reagent well 6112; it can be appreciated that the hole site can be used for placing different reagents and disposable consumables such as a magnetic sleeve according to specific experimental requirements, and the packaging film 612 can only seal the reagent hole site 6112 in consideration of convenience of subsequent suction head and magnetic sleeve taking. The outer wall of the cartridge 620 is further provided with a positioning member 622, and the positioning member 622 is correspondingly matched with the positioning member 123 for defining the position of the cartridge, for example, corresponding inclined planes can be provided to be mutually engaged.

The storage mechanism 100 includes a storage housing 110 and a drawer 120, a card pushing mechanism 130, and a locking mechanism 140 mounted within the storage housing 110, as shown in fig. 7-8.

The drawer 120 is provided with a receiving cavity for receiving the reagent card strip 610, and in this embodiment, the reagent card strip 610 is mounted in the cassette 620 and then placed in the drawer 120, as shown in fig. 9.

The card pushing mechanism 130 includes a card pushing driving device 131, a card pushing assembly 132 and a card pushing guide rail 133, wherein the card pushing assembly 132 is driven by the card pushing driving device 131 to move along the card pushing guide rail 133, so that the reagent card strip 610 placed in the accommodating cavity is pushed to the edge of the drawer 120, and is pushed to a position corresponding to the receiving mechanism.

As shown in fig. 10, in this embodiment, the card pushing assembly 132 includes a card pushing screw 1321, a card pushing nut 1322 and a card pushing member 1323, the card pushing driving device 131 is a motor, an output end of the motor is connected to the card pushing screw 1321, the card pushing nut 1322 is sleeved on the card pushing screw 1321 and is connected to the card pushing member 1323, and the card pushing member 1323 is slidably connected to the card pushing guide rail 133, so that the card pushing member 1323 moves along the guide rail 133 under the limit guidance of the guide rail 133, thereby achieving the card pushing purpose. It will be appreciated that to avoid rocking of push-clamp screw 1321, push-clamp screw 1321 is mounted and fixed by bearings.

The accurate and stable card pushing action of the card pushing mechanism 130 is facilitated in view of better positioning of the cartridge 620, i.e., ensuring that the reagent card strip 610 within the cartridge 620 is in the correct position. As shown in fig. 9, the drawer 120 is further provided with a positioning member 123 for defining the position of the cartridge 620, and the positioning member 123 in the drawer 120 (on which a slant is provided) cooperates with a positioning member 622 on the outer wall of the cartridge 620, so as to limit the placement of the cartridge 620 in the drawer 120 in the front-back, left-right positions.

The drawer 120 may be disposed in a closed state and an extracted state along a sliding rail in the storage housing 110, the outer wall of the drawer 120 is provided with a slot member 121, and a slot hole 1211 is formed in the slot member, in this embodiment, the locking mechanism 140 includes a latch 141, a latch driving device 142, a locking bracket 143, and an elastic member 144, when the drawer 120 is disposed in the closed state, the position of the latch 141 corresponds to the position of the slot hole 1211, and the latch 141 may be driven by the latch driving device 142 to insert into or withdraw from the slot hole 1211. Specifically, the head of the pin 141 is provided with an arc-shaped guiding surface 1411 (such as a cylindrical head), and the slot member 121 is provided with a guiding slope 1212; the plug 141 is mounted on the locking bracket 143 through a sliding bearing 145, one end of the elastic member 144 abuts against the plug 141, and the other end of the elastic member is fixed, so that the plug 141 has a tendency of being inserted into the slot hole 1211, the plug driving device 142 is an electromagnet, and is fixed on the locking bracket 143, and the output end of the electromagnet is connected with the plug 141 through a power connecting rod 1146, so that the plug 141 is driven to withdraw from the slot against the elastic force of the elastic member 144, as shown in fig. 11-12.

Specifically, when the locking mechanism 140 is powered off, the locking mechanism is always in an extended state under the action of the elastic member 144 (such as a spring), and when the drawer 120 is pushed in, the arc-shaped guide surface 1411 of the head of the plug pin 141 is pushed up along the guide slope 1212 of the periphery of the slot member 121 until the drawer 120 is pushed in to the closed state, the plug pin 141 corresponds to the slot hole 1211, and after the plug pin 141 is inserted into the slot hole 1211, the drawer 120 is locked. The electromagnet needs to be activated to pull the drawer 120 out. And a spring is installed on the latch 141 in order to prevent the spring from providing an elastic force to force the push-pull rod of the electromagnet to return when the push-pull rod of the electromagnet cannot return to the original position due to residual magnetism.

The electromagnet is in a power-off extending type, the plug 141 is driven by the electromagnet through the power connecting rod, so that the push-pull force of the drawer 120 directly acts on the push-pull rod of the electromagnet to cause the bending of the push-pull rod, and the service life of the electromagnet is prolonged. The latch 141 is mounted on the locking bracket 143 through the sliding bearing 145, so that sliding guiding of the latch 141 is ensured, and pushing and pulling force of the drawer 120 borne by the latch 141 can be transmitted to the locking frame 500 through the power connecting rod 1146, as shown in fig. 12-13.

The access mechanism 200 is disposed beside the storage mechanism 100, i.e. beside the drawer, and corresponds to the card pushing assembly, the reagent card strip moves to the edge of the drawer under the pushing of the card pushing assembly, and corresponds to the access mechanism exactly, so as to perform the next process.

As shown in fig. 14, the access mechanism 200 includes a lifting mechanism 210 and an access component 220, where the access component 220 can be driven by the lifting mechanism 210 to perform lifting motion, and has a card taking state position and a card feeding state position, the card taking state position is below the card feeding state position, and when the access component is located in the card taking state position, the access component corresponds to the drawer edge position.

As shown in fig. 15-16, the access assembly 220 includes an access substrate 221, a telescopic driving device 222, a telescopic assembly 223 and an access board 224, which are sequentially connected, the access assembly 220 is connected to the lifting mechanism 210 through the access substrate 221, the telescopic assembly drives the access board 224 to perform telescopic motion under the driving of the telescopic driving device 222, and the access board 224 is provided with an access piece 2241 matched with the reagent card strip 610.

As shown in fig. 17-19, in this embodiment, the receiving member 2241 is a receiving strip extending from the receiving plate 224 toward the reagent card strip 610; the access panel 224 and the extended access member together form a U-shaped opening into which the package housing can be accessed while avoiding the cartridge 620. When the access panel 224 is raised, the reagent strip 610 is lifted out of the cassette 620. And, the receiving part is provided with a limit slot 2241a for limiting the position of the reagent card strip 610. The limiting slot 2241a is configured to limit movement of the reagent card strip 610 on the access panel 224 to prevent the reagent card strip 610 from falling during transportation.

Specifically, the telescopic driving device 222 is a linear motor, and the telescopic component is a linear bearing. To precisely control the working process of the device, the access assembly 220 further includes a telescopic optocoupler 225 for identifying the position of the access panel 224. The lifting mechanism 210 comprises a lifting optocoupler, a lifting driving device and a lifting driving belt 213, wherein the lifting optocoupler is used for identifying the position of the access assembly 220, and the lifting optocoupler can be realized through a lifting light rail 211 arranged on the access assembly, the lifting driving device drives the lifting driving belt 213 to move, and the access assembly 220 is fixed on the lifting driving belt 213.

The conveying mechanism 300 includes a conveying seat 310, a conveying driving device 320, a conveying member 330 and a conveying guide rail 340, as shown in fig. 20, the conveying seat 310 corresponds to a card feeding state position of the access assembly, the conveying member 330 moves along the conveying guide rail 340 under the driving of the conveying driving device 320, the conveying seat 310 is fixed on the conveying member 330, and a placing table 311 for placing the reagent card strip 610 is arranged on the conveying seat 310. The conveying seat 310 is driven by the conveying member 330 to move along the conveying guide 340 to a piercing position corresponding to the film piercing mechanism 400, a liquid transferring position corresponding to the liquid transferring device, and a waiting position waiting for a next process.

Similarly, the carrier 310 further includes a level sensor 312 and a waiting sensor 313, wherein when the carrier 310 is at the level, the level sensor 312 is triggered to generate a signal, and when the carrier 310 is at the waiting position, the waiting sensor 313 is triggered to generate a signal. The pipetting level corresponds to the mounting position of pipetting mechanism 700.

21-22, the film puncturing mechanism 400 comprises a puncturing assembly 410, a film puncturing driving device 420, a film puncturing fixing frame 430, a film puncturing screw 440 and a film puncturing slide block 450, wherein a puncturing head 411 is arranged on the puncturing assembly 410, the film puncturing driving device 420 is installed on the film puncturing fixing frame 430, the film puncturing screw 440 is connected with the output end of the film puncturing driving device 420, the film puncturing slide block 450 is sleeved on the film puncturing screw 440, the puncturing assembly 410 is fixed on the film puncturing slide block 450, and the puncturing head 411 can perform downward puncturing movement and reset under the driving of the film puncturing driving device 420 on the reagent clamping strips 610 on the opposite table 311.

Specifically, the piercing head 411 has a solid triangular prism structure. The puncture head 411 has the above structure, and is advantageous in that it punctures the packaging film 612 and is easily separated from the reagent card strip 610.

In this embodiment, the film punching mechanism 400 further includes a pre-pressing module 460, as shown in fig. 23, where the pre-pressing module 460 includes a pre-pressing block 461, a pre-pressing elastic member 462 (spring), a pressing block guiding shaft 463, a pre-pressing stop 464, and a pre-pressing support 465, where a guiding hole is provided on the pre-pressing support 465, the pre-pressing guiding shaft penetrates through the guiding hole, one end of the pre-pressing guiding shaft above the pre-pressing support 465 is a top end, one end of the pre-pressing guiding shaft below the pre-pressing support 465 is a bottom end, and the pre-pressing stop 464 has a diameter larger than that of the guiding hole, and is mounted on the top end of the pre-pressing guiding shaft to avoid the pressing block guiding shaft from sliding out from the guiding hole, the pre-pressing block 461 is mounted on the bottom end of the pre-pressing guiding shaft, one end of the pre-pressing elastic member 462 abuts against the pre-pressing support 465, and the other end of the pre-pressing block 461 has a downward movement tendency.

The pre-pressing modules 460 may be divided into two groups, and are respectively disposed at the left and right ends of the reagent card strip 610. When puncturing, the pre-pressing module 460 firstly contacts the reagent card strip 610 and applies a certain pressure to prevent the reagent card strip 610 from moving; the puncture head 411 then punctures the encapsulation film 612. After lancing, the puncture head 411 is raised and after completely leaving the non-contact reagent strip 610, the pre-compression block 461 of the pre-compression module 460 begins to come out of contact with the reagent strip 610. This prevents the piercing tip 411 from carrying the reagent card strip when it is disengaged from the reagent card strip 610.

In this embodiment, the pushing card assembly 132, the lifting mechanism 210, the access assembly 220, the conveying seat 310, the puncture assembly 410, and the like are two sets to cooperate with the simultaneous processing of two reagent card strips 610; the drawers 120 are two in number and are placed one above the other in the storage housing 110.

By increasing the number of push card assemblies 132, lifting mechanisms 210, access assemblies 220, transport holders 310, and piercing assemblies 410, multiple channels are allowed to operate simultaneously, improving automatic loading efficiency. The large volume of the drawers is utilized to increase the quantity of the reagent card strips 610, so that the time interval for manually replenishing the reagent card strips 610 is increased, and the reagent card strips are also beneficial to independent operation and no interference in the working process, when one of the two drawers 120 is in shortage of materials, the other drawer 120 is not influenced to continue to work, and the problem that the equipment must be stopped for replenishing materials again when the materials are used up is solved; or one of the channels fails, the other set of mechanisms can continue to operate.

The storage mechanism 100, the access mechanism 200, the conveying mechanism 300 and the film puncturing mechanism 400 are all installed on the frame 500, the storage mechanism 100 is arranged below the access mechanism 200, and the conveying mechanism 300 is arranged between the access mechanism 200 and the film puncturing mechanism 400.

Example 2

An automatic feeding method for reagent card strips, which adopts the automatic feeding equipment for the reagent card strips and the reagent card strip assembly of the embodiment 1, comprises the following steps:

1. charging material

The user manually operates, draws the drawer out, then takes out the packing lid of whole set of cartridge, puts into the drawer with the cartridge, makes the screens piece of cartridge and the setting element inclined plane of drawer agree with each other, installs in place, pushes into working position with the drawer at last. Thus, the manual operation is completed.

2. Push card

The card pushing mechanism sequentially pushes the reagent card strips stored in the card boxes to the edges of the drawers one by one, and the positions correspond to the receiving mechanism (namely, the position A).

3. Lifting up

As shown in fig. 24, the lifting mechanism drives the access assembly to descend to the position a, the telescopic assembly stretches the access plate out to the position below the reagent card strip at the outermost side of the card box, the access piece is located below the clamping piece of the reagent card strip, the lifting mechanism drives the access assembly to ascend a distance, two ends of the reagent card strip are separated from the card box package, the reagent card strip is fetched, the telescopic assembly drives the access plate to retract, the lifting mechanism continues to lift the access assembly to the position (position B) corresponding to the film penetrating mechanism, the telescopic assembly stretches the access plate, the reagent card strip is located at a position slightly higher than the conveying seat, the lifting mechanism drives the access assembly to descend, the clamping piece of the reagent card strip is placed on the placing table of the conveying seat, so that the action of placing the reagent card strip on the conveying seat is completed, the telescopic assembly drives the access plate to retract, and the lifting mechanism drives the access assembly to descend to the position a, and the other reagent card strip is fetched; and so on.

4. Puncture needle

The conveying mechanism conveys the reagent card strip to the puncture position. A puncturing film driving device in the puncturing film mechanism drives a puncturing head to move downwards, and a pre-pressing block of the pre-pressing module firstly contacts the reagent clamping strip and applies certain pressure before the puncturing head contacts the reagent clamping strip packaging film so as to prevent the reagent clamping strip from moving; then the puncture head continues downwards, the pre-pressing module is fixed relative to the clamping strip, the spring is compressed, the pressing block guide shaft moves upwards in the guide hole relative to the pre-pressing support, and the puncture head continues downwards to puncture the packaging film. After puncturing, the puncture head rises, the pre-pressing module still contacts and pushes the reagent card strip under the action of the spring, the puncture head rises, and after completely leaving the reagent card strip which is not contacted, the pre-pressing module starts to break away from contact with the reagent card strip and returns to the original position.

5. Pipetting liquid

As shown in fig. 25, the transport mechanism transports the reagent card strip that pierces the packaging film (aluminum film) to the shift level (position C). And the pipette tip is matched with the pipetting mechanism to take the pipette tip from a pipette tip storage hole position in the reagent card strip. The pipetting mechanism moves to the upper part of a sample tube (not shown in the figure), sucks the sample through the suction head, and returns to the upper part of the reagent card strip to finish the actions of releasing, blowing, mixing, pipetting again and the like. The pipetting mechanism puts the used suction head back into the original hole site of the reagent card strip.

6. Waiting for position

As shown in fig. 25, the transport mechanism transports the reagent card strip having completed the pipetting operation to a waiting position (position D), and waits for the mechanism operation of the next station. Thus, the full-automatic flow of the present embodiment is completed.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (14)

1. Reagent card strip automatic feeding equipment, its characterized in that includes:

the storage mechanism comprises a storage shell, and a drawer, a card pushing mechanism and a locking mechanism which are arranged in the storage shell, wherein a containing cavity for containing the reagent card strip is arranged in the drawer, the card pushing mechanism comprises a card pushing driving device, a card pushing assembly and a card pushing guide rail, and the card pushing assembly moves along the card pushing guide rail under the driving of the card pushing driving device so as to push the reagent card strip placed in the containing cavity to the edge of the drawer; the drawer can be respectively positioned at a closed state position and an extracted state position along the sliding rail in the storage shell in a pulling manner, the outer wall of the drawer is provided with a slot piece, a slot hole is formed in the slot piece, the locking mechanism comprises a bolt and a bolt driving device, when the drawer is positioned at the closed state position, the position of the bolt corresponds to the position of the slot hole, and the bolt can be driven by the bolt driving device to be inserted into or withdrawn from the slot hole;

the collecting mechanism comprises a lifting mechanism and a collecting component, the collecting component can be driven by the lifting mechanism to do lifting motion and is provided with a card collecting state position and a card feeding state position, the card collecting state position is positioned below the card feeding state position, when the collecting component is positioned at the card collecting state position, the collecting component corresponds to the edge position of the drawer, the collecting component comprises a collecting base plate, a telescopic driving device, a lifting component and a collecting plate which are sequentially connected, the collecting component is connected with the lifting mechanism through the collecting base plate, the telescopic component is driven by the telescopic driving device to drive the collecting plate to do telescopic motion, and a collecting piece matched with a reagent card strip is arranged on the collecting plate;

the conveying mechanism comprises a conveying seat, a placing table for placing reagent card strips is arranged on the conveying seat, and the conveying seat corresponds to a card feeding state position of the receiving component; and

the puncturing mechanism comprises a puncturing assembly and a puncturing film driving device, wherein a puncturing head is arranged on the puncturing assembly, and can downwards puncture and reset the reagent card strip on the opposite table under the driving of the puncturing film driving device.

2. The automatic reagent card strip feeding device according to claim 1, wherein the locking mechanism further comprises a locking bracket and an elastic piece, the bolt is slidably mounted on the locking bracket, one end of the elastic piece abuts against the bolt, the other end of the elastic piece is fixed, the bolt has a trend of being inserted into the slot hole, the bolt driving device is an electromagnet and is fixed on the locking bracket, the output end of the electromagnet is connected with the bolt, and the bolt is driven to withdraw from the slot against the elastic force of the elastic piece.

3. The automatic reagent card strip feeding device according to claim 1, wherein the pin head is provided with an arc-shaped guide surface, and the slot piece is provided with a guide slope;

the output end of the electromagnet is connected with the bolt through a power connecting rod;

the bolt is arranged on the locking bracket through a sliding bearing.

4. The automatic reagent card strip feeding equipment according to claim 1, wherein the card pushing assembly comprises a card pushing screw rod, a card pushing nut and a card pushing piece, the card pushing driving device is a motor, the output end of the motor is connected with the card pushing screw rod, the card pushing nut is sleeved on the card pushing screw rod and connected with the card pushing piece, and the card pushing piece is connected with the card pushing guide rail in a sliding manner;

and a positioning piece used for limiting the position of the reagent card strip is also arranged in the drawer.

5. The automatic reagent card strip feeding device of claim 1, wherein the receiving member is a receiving strip extending from the receiving plate in a direction of the reagent card strip;

the telescopic driving device is a linear motor, and the telescopic component is a linear bearing;

the access assembly further comprises a telescopic optocoupler for identifying the position of the access plate;

the lifting mechanism comprises a lifting optocoupler, a lifting driving device and a lifting driving belt, wherein the lifting optocoupler is used for identifying the position of the receiving component, the lifting driving device drives the lifting driving belt to move, and the receiving component is fixed on the lifting driving belt.

6. The automated reagent card strip loading device of claim 5, wherein the receiving member is provided with a limiting slot for limiting the position of the reagent card strip.

7. The automatic reagent card strip feeding device according to claim 1, wherein the conveying mechanism further comprises a conveying driving device, a conveying member and a conveying guide rail, the conveying member moves along the conveying guide rail under the driving of the conveying driving device, and the conveying seat is fixed on the conveying member.

8. The automatic reagent card strip feeding equipment according to claim 7, wherein the conveying seat can move to a puncture position corresponding to the film puncturing mechanism, a liquid moving level corresponding to the liquid moving equipment and a waiting position waiting for the next process along the conveying guide rail under the drive of the conveying piece;

the conveying seat further comprises a liquid level shifting sensor and a waiting position sensor, when the conveying seat is positioned at the liquid level shifting position, the liquid level shifting sensor is triggered to generate signals, and when the conveying seat is positioned at the waiting position, the waiting position sensor is triggered to generate signals.

9. The automatic reagent card strip feeding equipment according to claim 1, wherein the film puncturing mechanism further comprises a film puncturing fixing frame, a film puncturing screw rod and a film puncturing sliding block, the film puncturing driving device is arranged on the film puncturing fixing frame, the film puncturing screw rod is connected with the output end of the film puncturing driving device, the film puncturing sliding block is sleeved on the film puncturing screw rod, and the puncturing assembly is fixed on the film puncturing sliding block;

the puncture head is of a solid triangular prismatic structure.

10. The automatic reagent card strip feeding equipment according to claim 1, wherein the film punching mechanism further comprises a pre-pressing module, the pre-pressing module comprises a pre-pressing block, a pre-pressing elastic piece, a pressing block guide shaft, a pre-pressing stop block and a pre-pressing support, a guide hole is formed in the pre-pressing support, the pre-pressing guide shaft penetrates through the guide hole, one end of the pre-pressing guide shaft, which is located above the pre-pressing support, is a top end, one end of the pre-pressing guide shaft, which is located below the pre-pressing support, is a bottom end, the maximum radial distance of the pre-pressing stop block is greater than the diameter of the guide hole and is mounted at the top end of the pre-pressing guide shaft, one end of the pre-pressing elastic piece abuts against the pre-pressing support, and the other end of the pre-pressing elastic piece abuts against the pre-pressing block, so that the pre-pressing block has a downward movement trend.

11. The automatic reagent card strip feeding device of claim 1, wherein the pushing card assembly, the lifting mechanism, the receiving assembly, the conveying seat and the puncturing assembly are two sets to cooperate with simultaneous processing of two reagent card strips;

and/or

The number of drawers is two, and the drawers are placed in the storage shell in an up-down overlapping mode.

12. A reagent card strip assembly, which is characterized by comprising a reagent card strip matched with the automatic feeding equipment of any one of claims 1-11, wherein a packaging film is arranged at the top of the reagent card strip, and clamping pieces matched with the receiving pieces and the placing table are arranged at two ends of the reagent card strip.

13. The reagent card strip assembly of claim 12, further comprising a card box, wherein a plurality of reagent card strips are arranged in the card box, and clamping pieces at two ends of the reagent card strips extend to the outer edge of the top of the card box and are in a hook shape with downward openings;

the hole site includes: tip and reagent sites;

the outer wall of the card box is also provided with a clamping piece, and the clamping piece is correspondingly matched with a positioning piece which is arranged in the storage shell and used for limiting the position of the card box.

14. A method for automatically feeding a reagent card strip, characterized in that the reagent card strip automatic feeding device according to any one of claims 1 to 11 and the reagent card strip according to any one of claims 11 to 13 are adopted, comprising the following steps:

pushing cards: placing the reagent card strips in a containing cavity of a drawer, and pushing the reagent card strips to the edge of the drawer under the action of the card pushing assembly;

lifting: the lifting mechanism drives the receiving component to descend to a preset position, the receiving plate stretches out to the lower side of the reagent clamping strip under the drive of the telescopic component, the receiving piece on the receiving plate is matched with the clamping piece of the reagent clamping strip, the lifting mechanism drives the receiving component to ascend, and the receiving piece lifts the reagent clamping strip to the preset position;

puncturing: the puncturing film driving device drives the puncturing head to puncture downwards, punctures the packaging film of the reagent card strip, and resets the puncturing head.

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