CN110327659A - A kind of extraction and purification method and device based on magnetic fiber material - Google Patents
A kind of extraction and purification method and device based on magnetic fiber material Download PDFInfo
- Publication number
- CN110327659A CN110327659A CN201910653729.1A CN201910653729A CN110327659A CN 110327659 A CN110327659 A CN 110327659A CN 201910653729 A CN201910653729 A CN 201910653729A CN 110327659 A CN110327659 A CN 110327659A
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- extraction
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- fiber material
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- magnetic fiber
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- 238000000605 extraction Methods 0.000 title claims abstract description 218
- 239000002657 fibrous material Substances 0.000 title claims abstract description 111
- 238000000746 purification Methods 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 113
- 239000000696 magnetic material Substances 0.000 claims abstract description 3
- 238000003860 storage Methods 0.000 claims description 35
- 239000000463 material Substances 0.000 claims description 32
- 230000005540 biological transmission Effects 0.000 claims description 24
- 230000005389 magnetism Effects 0.000 claims description 24
- 230000007246 mechanism Effects 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 17
- 230000004913 activation Effects 0.000 claims description 14
- 230000033001 locomotion Effects 0.000 claims description 14
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 238000010828 elution Methods 0.000 claims description 12
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- 238000004587 chromatography analysis Methods 0.000 claims description 4
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- 238000004895 liquid chromatography mass spectrometry Methods 0.000 claims description 4
- 239000002861 polymer material Substances 0.000 claims description 4
- 238000005253 cladding Methods 0.000 claims description 2
- 229910010272 inorganic material Inorganic materials 0.000 claims description 2
- 239000011147 inorganic material Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims 1
- 239000000523 sample Substances 0.000 description 24
- 239000000284 extract Substances 0.000 description 16
- 239000007788 liquid Substances 0.000 description 15
- 239000012488 sample solution Substances 0.000 description 11
- 238000004458 analytical method Methods 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 238000001179 sorption measurement Methods 0.000 description 8
- 239000002699 waste material Substances 0.000 description 8
- 230000003213 activating effect Effects 0.000 description 7
- 239000003480 eluent Substances 0.000 description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 4
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 230000005611 electricity Effects 0.000 description 4
- 238000002203 pretreatment Methods 0.000 description 4
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- 239000000126 substance Substances 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- QCWXUUIWCKQGHC-UHFFFAOYSA-N Zirconium Chemical compound [Zr] QCWXUUIWCKQGHC-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
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- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000008570 general process Effects 0.000 description 2
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- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229920000058 polyacrylate Polymers 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229910052726 zirconium Inorganic materials 0.000 description 2
- 229920002125 Sokalan® Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28009—Magnetic properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Abstract
The present invention relates to a kind of extraction based on magnetic fiber material and purification method and device, the extraction and purification method are the following steps are included: bar magnet is powered and draws magnetic fiber material by (1)；(2) bar magnet is moved at extraction station module G1, bar magnet power-off, magnetic fiber material is detached from bar magnet and falls into corresponding container, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, first step of extraction process is completed, bar magnet is powered and adsorbs magnetic fiber material again；Or bar magnet is in energized state, rotates in a reservoir with magnetic material, completes first step of extraction process；(3) bar magnet moves at extraction station module G2, bar magnet power-off, magnetic fiber material is detached from bar magnet and falls into corresponding container, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, the second step of extraction process is completed, bar magnet is powered and adsorbs magnetic fiber material again.
Description
Technical field
The invention belongs to external clinical detection sample process fields, and in particular to a kind of extraction based on magnetic fiber material
With purification method and device.
Background technique
In clinical examination and accurate medical field, the instruments such as mass spectrum, chromatography or LC-MS instrument are in sensitivity, general
Property, analysis speed, multi objective detect etc. simultaneously has advantage, these high-accuracy analysis instruments can be applied to clinical life
Change, the fields such as clinical immunization is examined, clinical microbiology laboratory technique and proteomics, metabolism group scientific research.However, high-accuracy
Analysis instrument is stringenter to the pre-treatment requirement of test sample, in addition, due to the complexity of biological detection sample, so that sample
The difficulty of pre-treatment is higher, and still have following problem to be solved: (1) Sample pretreatment step is various, process is complicated, to personnel
Skill requirement is high；(2) non intelligentization, human interference factor is big, easily causes that unstable result, error are big, error rate is high；(3) real
Test that the period is long or treatment effeciency is lower, it is at high cost.
Purifying treatment method and realization rate of the mainly current sample of the reason of causing these problems before entering mass spectrum
It is more complicated, and the time of sample treatment is longer, limit the development of high-accuracy analysis instrument detection industry in vitro.Separately
Outside, traditional extraction is that solid adsorption material is filled in extraction column, forms stationary phase, and stationary phase respectively has one piece of filtering up and down
Plate, sample solution to be analyzed flow through stationary phase, and selective extraction or absorption are detected ingredient or interfering substance, to reach separation
With the purpose of purification.The shortcomings that extracting column extracting is the difference or sample solution viscosity due to filling and being compacted elasticity
Difference or pressurization/vacuum difference cause sample solution flow velocity to generate larger difference, influence stationary phase in sample solution
The Dynamic Adsorption of key component causes final detection result to have very big error.Additionally due to liquid flow is out-of-date when partial size is smaller
Resistance can be very big, so the partial size of filler is usual larger (usually at 50 microns or more) in solid-phase extraction column；However when partial size compared with
When big, sample mass transfer velocity can be very slow, therefore sample crosses column speed otherwise cannot reach effective absorption quickly.
Dispersive solid-phase extraction disperses solid adsorption material in sample solution, concussion a period of time, makes in sample solution
Key component reach between liquid phase and solid phase Thermodynamic Adsorption balance, selection is reached according to the characteristic of solid adsorption material
Property adsorb the purpose of tested ingredient or impurity.This method eliminates the detections generated in extraction column extracting due to flow velocity difference
Resultant error.But the shortcomings that this method is: 1) target substance in fluid sample reaches absorption in fiber material and solution
Balance needs the long period；2) process separated solid phase with solution is more complicated.
Pervious device mostly uses greatly the pattern of XYZ axis, and processing speed is slow, and mass spectrum is allowed to be constantly in the shape for waiting sample
State improves processing speed, mass spectrum still interval time is longer although handling simultaneously by multiple channels to a certain degree.
For handling 96 samples, pre-treatment instrument processing after the completion of cannot be transported to mass spectrograph at once, need to wait 96 simultaneously
After having handled, and mass spectrograph is to be detected one by one, leads to first sample and the last one sample intervals very
It is long.It is not able to satisfy the demand of current clinical detection.
Summary of the invention
In order to achieve the above object, the present invention provides a kind of extraction based on magnetic fiber material and purification method and dresses
Set, by the way of bidirectional crossed operation, handle sample interval time be 3-5 minute, just meet mass spectrograph detect when
Between, the waiting time is not present in this way.
Auto extractive and purification device of the invention includes at least one extraction module, at least three station modules and first
Guide rail, the extraction module or station module can move on the first guide rail, and the station module is arranged under extraction module
Side, and including at least one odd-job position module and at least two extraction station modules, station module along the first guide rail movement
Direction sequence arranges, and magnetic fiber material is equipped in station module.The extraction module includes bar magnet, power supply and the second guide rail,
The bar magnet connects power supply, and can move up and down on the second guide rail, and the power supply control bar magnet is to the magnetic fiber material
Absorption and disengaging.
The extraction module includes bar magnet, power supply and the second guide rail, and second guide rail connects and is able to drive bar magnet and does
It moves up and down, the bar magnet is connect by circuit with the power supply.When power supply is that bar magnet is powered, the bar magnet has magnetism,
And magnetic fiber material can be attracted, the magnetism fiber material is adsorbed on the outer surface of bar magnet, and can be mobile with bar magnet.When
When power outage, the magnetic of the bar magnet disappears, and the magnetism fiber material is detached from bar magnet.
The bar magnet protrudes the lower surface of the extraction module, and can extend into the reagent bottle of the station module, excellent
Choosing, the bar magnet is cylinder, according to the extraction module at a distance from station module and the depth of reagent bottle, selection magnetic
The length of stick.Preferably, the position of 2/3rds height of reagent bottle is protruded into the bar magnet bottom end.
The magnetism fiber material is granular material, and magnetic fiber material is selected from non-hud typed conventional magnetic and extracts material
Material and hud typed mesoporous magnetic fiber material.When the power supply is that bar magnet is powered, the magnetism fiber material is adsorbed on magnetic
The outer surface of stick, and can be moved to bar magnet in the reagent bottle of different station module；When power outage, the bar magnet
It is magnetic disappear, the magnetism fiber material is detached from bar magnet, fall into reagent bottle, under stirring and/or concussion effect, magnetism extraction
It draws materials and is come into full contact with reagent solution, complete the different step of extraction process；When magnetic fiber material is contacted with reagent solution
Sufficiently after, bar magnet is once again powered up, and magnetic fiber material is adsorbed on bar magnet, and with bar magnet and extraction module be moved to together it is next
A station module.
Preferably, the magnetic fiber material is selected from hud typed mesoporous magnetic fiber material, to accelerate extraction equilibrium
Speed, to accelerate entire extraction process；The middle part of the hud typed mesoporous magnetic fiber material is solid or has micro-
The average pore size of the inorganic material or high polymer material in hole, the micropore is less than 3nm；The hud typed mesoporous magnetic extraction material
The shell cladding of material has mesoporous inorganic or high polymer material, and the mesoporous average pore size is 3-50nm, it is preferred that described
Mesoporous average pore size is 6-20nm.
The middle part nuclear material of the magnetism fiber material is mainly magnetic iron oxide, can also include silica gel, aluminium oxide, oxygen
Change the combination of one or more of zirconium, polystyrene, polyacrylate, cellulose, starch.
The sheathing material of the magnetism fiber material is selected from mesoporous silica gel, mesoporous bonded silica gel, meso-porous alumina, mesoporous oxygen
Change zirconium, mesoporous polystyrene, mesopore surfaces modified polystyrene, mesoporous polyacrylic acid, one kind of mesoporous polyacrylate or two
Kind or more combination.
The magnetism fiber material is placed in the module of the odd-job position, it is preferred that the equal equal portions of the magnetism fiber material
Ground is placed in each reagent bottle in the module of odd-job position.
For the power supply by bar magnet described in circuit connection, the power supply is selected from indoor exchange electricity consumption or high-power battery.
Preferably, the top of bar magnet is arranged in the power supply, and second guide rail is directly connected to power supply, and drive power supply and
Bar magnet moves up and down together.
Preferably, the outside of the extraction module is arranged in the power supply, and the second guide rail connects the bar magnet or support magnetic
The component of stick moves up and down so that the second guide rail is able to drive bar magnet.
In the specific embodiment of the present invention, the top of bar magnet is arranged in the power supply, and second guide rail is straight
Power supply is connect in succession, and power supply and bar magnet is driven to move up and down together.
It is furthermore preferred that each extraction module is equipped with a power supply, convenient for controlling different extraction modules respectively for magnetic
Property fiber material attraction or time for not attracting because difference extraction modules correspond to different station module, and execute and extracted
When the different step of journey, it is desirable that magnetic fiber material is different from the time that reagent comes into full contact with, for example, extraction activation, absorption,
The time of elution is different, and the power supply of corresponding extraction module can individually control an extraction module, so that operation is more flexible
Accurately, extraction efficiency is improved.
Preferably, the extraction module is equipped with one or both of rotating device or shaking device, the rotating device
And/or shaking device is connect with the bar magnet, and bar magnet can be driven to rotate and/or shake, and bar magnet is made to play stirring and/or vibration
Effect, promote coming into full contact with for the magnetic fiber material and reagent solution, improve extraction processing efficiency.
Preferably, the upper and lower part of second guide rail is equipped with limit switch, and the limit switch prompts and controls magnetic
The upper and lower bound position of rod motion, prevents bar magnet and/or power supply is more than normal moving range, destroys under the extraction module
The station module of side, or destroy the first guide rail.
The auto extractive and purification device further include liquid level sensor, and the liquid level sensor includes pressure sensing
Device and capacitance sensor, the liquid level sensor detect the liquid level for the reagent bottle that the bar magnet protrudes into, and guarantee bar magnet each
Under the liquid level for submerging the reagent bottle at a station module, avoid bar magnet not in contact with the reagent in the reagent bottle of station module,
Or bar magnet gos deep into very much the bottom of bottle of reagent bottle and bursting reagent bottle, or mistake occur in extraction module and the position of station module, and
So that bar magnet and reagent bottle misplace.
Preferably, the middle part or lower part of the second guide rail is arranged in the liquid level sensor.Preferably, the level detection
The upper surface of the station module is arranged in device.
Preferably, the quantity of the extraction module is 2-100, meets the difference of small-sized, medium-sized and large-scale detection and analysis
It needs.For small-sized or medium-sized detection and analysis, the quantity of the extraction module is preferably 3-6, can meet extracting substances
General process, that is, activate, extract, elute and elute and etc..
The position of the first guide rail setting is selected from the top of the extraction module or the lower section of station module, the extraction
Module or station module connect first guide rail, and can move on the first guide rail.
Preferably, the top of the extraction module is arranged in first guide rail, and the top of second guide rail is detachable
Ground is fixed on the first guide rail, when the auto extractive and purification device include multiple extraction modules, multiple extraction modules according to
It is secondary to be arranged sequentially on the first guide rail.
Preferably, the lower section of the station module is arranged in first guide rail, and the bottom of station module is removably solid
It is scheduled on the first guide rail, when the auto extractive and purification device include multiple station modules, multiple station modules are successively suitable
Sequence is arranged on the first guide rail.At this point, the second guide rail can be fixed on the bracket beside the first guide rail, guarantee the extraction mould
It the position of block and station module can be opposite up and down.
The shape of first guide rail is selected from bar shaped or annular, it is preferred that first guide rail is ring-shaped guide rail, Duo Gecui
Modulus block or station module do circular motion on ring-shaped guide rail, are easy to implement continuous extraction operation.
The lower section of the extraction module, including at least one odd-job position module and at least two is arranged in the station module
It extracts station module, odd-job position module and extraction station module to arrange along the direction of motion sequence of the first guide rail, i.e. institute
The position for stating station module can be corresponding up and down with the position of the extraction module.
The station module includes turntable, transmission mechanism and stores disk, and the storage pan bottom hollow out and side, which are equipped with, opens
Mouthful, the transmission mechanism is below storage disk, and the edge of the turntable is equipped with the detent of at least one recess, the storage disk
It is open and corresponds to the detent of turntable.
The station module further includes driving motor, and the driving motor drives the turntable to rotate in the horizontal plane, institute
Stating detent has opening in the position of disk edge, for being clamped reagent bottle.
The transmission mechanism supports the reagent bottle inside storage disk below storage disk.
Pile reagent bottle in the storage disk, the reagent bottle by transmission mechanism support, and in the drive of transmission mechanism
The opening of the lower harness motion to storage disk, the storage disk only allows a reagent bottle to pass through, and previous reagent bottle is latter
The opening of storage disk is removed under the promotion of a reagent bottle and enters the detent of turntable, after the detent receives a reagent bottle,
Under the drive of the driving motor, suitable position is turned to turntable, waits and docks the extraction module.
Preferably, the transmission mechanism is conveyer belt.
Preferably, the lower section of the turntable is arranged in the driving motor, and turntable is driven to rotate in the horizontal plane, simultaneously
Detent described in driven by rotary disc turns to different location.
Preferably, the transmission mechanism and storage disk are located at the side of turntable, and the opening of the storage disk is corresponding or docks
The opening of the detent of turntable.
Preferably, the storage disk is framework only with side frame, that is, stores the top and bottom hollow out of disk, work as institute
When stating transmission mechanism operating, storage disk position is fixed, and only the reagent bottle in storage disk is moved with transmission mechanism.
Preferably, the station module further includes waste box, and the waste box is located at the lower section of the turntable, more excellent
Choosing, the waste box is located at the lower section of the detent corresponding position of the turntable, convenient for accepting the reagent bottle fallen from detent.
Preferably, the device for pushing and abandoning reagent bottle is equipped at the detent of the turntable.
In use, holding a certain amount of magnetic extraction material in the reagent bottle placed inside the storage disk of odd-job position module
Material, the physico-chemical property of various solution according to used in actual treatment sample and extraction process determine magnetic in each reagent bottle
The amount of fiber material.
In use, reagent bottle is placed inside the storage disk of the extraction station module, according to each extraction station mould
The difference for the extraction step that block is undertaken, the reagent in the reagent bottle of each extraction station module is different, makees for example, undertaking activation
It extracts in whole reagent bottles of station module and all holds activating solution, undertake whole examinations of the extraction station module of suction-operated
Sample solution is all held in agent bottle, undertakes in whole reagent bottles of the extraction station module of soil washing and all holds eluent.
Preferably, the quantity of odd-job position module is 1-5, can be adjusted according to the sample total number of actual treatment.
Preferably, the extraction station module packet layout, every group of extraction station module complete the extraction of a sample to be tested
The general process taken, that is, activate, extract, elute and elute and etc., it is preferred, therefore, that one group of extraction station module for including
Quantity be 2-5.
It is furthermore preferred that the group number of the extraction station module is 1-10 group, meet small-sized, medium-sized and large-scale detection and analysis
Difference needs.For small-sized detection and analysis, the group number of the extraction station module is preferably 1-3 group.
Preferably, the station module is equipped with rocking equipment, and the rocking equipment connects the turntable or driving motor, and
Coming into full contact with for the concussion of energy band turn disk, the promotion magnetic fiber material and reagent solution, improves extraction processing efficiency.It is more excellent
Choosing, the lower section of the turntable or driving motor is arranged in the rocking equipment.
The auto extractive and purification device further include control device, the control device include governor circuit and control by
Key, the governor circuit pass through circuit connection and control first guide rail, the second guide rail, limit switch, power supply, transmission mechanism
And driving motor, it is preferred that the governor circuit, which also passes through circuit connection and controls the promotion at the detent of the turntable, to be abandoned
The device of reagent bottle.The control button connects governor circuit, and operator is facilitated to operate the control device.
Preferably, the control device can also have the function of that self-checking function and sample introduction, the self-checking function are that self-test is complete
Whether portion's extraction module completes the whole of extraction step, and each component is with requiring step and the auto extractive and purification device
No normal operation.The sample introduction function is to complete the reagent bottle of elution by sampling device, coherent detection instrument is sent into, after progress
Continuous detection, the sampling device select commercially available matched sampling device according to the type and model of actually detected instrument.
Reagent bottle of the present invention can be replaced by other containers, for example, kit, beaker, centrifuge tube etc., for containing
Put the solution such as magnetic fiber material, activating solution, sample solution, eluent or leacheate.
The present invention also provides a kind of extraction and purification method based on magnetic fiber material, the extraction and purification method
The following steps are included:
(1) bar magnet is powered and draws magnetic fiber material；
(2) bar magnet is moved at extraction station module G1, the bar magnet power-off, magnetic fiber material is detached from bar magnet and falls
Enter in corresponding container, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, complete the of extraction process
One step, bar magnet are powered and adsorb magnetic fiber material again；Or bar magnet is in energized state, with magnetic material in container
First step of extraction process is completed in middle rotation；
(3) bar magnet moves at extraction station module G2, and the bar magnet power-off, magnetic fiber material is detached from bar magnet and falls into
In corresponding container, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, complete the second of extraction process
A step, bar magnet are powered and adsorb magnetic fiber material again；
(4) optionally, the bar magnet moves at extraction station module Gn, the bar magnet power-off, and magnetic fiber material is de-
It from bar magnet and falls into corresponding container, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, complete extraction
N-th of step of process is taken, bar magnet is powered and adsorbs magnetic fiber material again；
N is selected from the integer of 3-20, e.g. 3,4,5,6,8,10,12,15,18,20；
Each step includes absorption and at least once elution step at least once in the extraction process.
Preferably, each step further includes an activation step or a rinsing step in the extraction process.
It is furthermore preferred that each step includes primary activation, primary absorption, primary elution and once washes in the extraction process
De- step.
For example, extracting in the container of station module G1 is sample solution in the above method, the appearance of station module G2 is extracted
It is eluent in device, extracts in the container of station module Gn as activating solution, leacheate etc..Heretofore described activating solution is washed
De- liquid and leacheate are selected according to the physico-chemical property of actually detected sample.
Preferably, when the extraction and purification method are used on auto extractive and purification device of the present invention, institute
State method the following steps are included:
(1) it is described extraction module by first guide rail move to odd-job position module position or the odd-job
Position module moves to the position of the extraction module by first guide rail, and the bar magnet is powered and draws magnetic extraction material
Material；
(2) for the extraction block motion at extraction station module G1, the bar magnet power-off, magnetic fiber material is detached from magnetic
Stick is simultaneously fallen into corresponding reagent bottle, and bar magnet rotation and/or vibration come into full contact with magnetic fiber material with corresponding reagent, complete
At first step of extraction process, bar magnet is powered and adsorbs magnetic fiber material again；
(3) for the extraction block motion at extraction station module G2, the bar magnet power-off, magnetic fiber material is detached from magnetic
Stick is simultaneously fallen into corresponding reagent bottle, and bar magnet rotation and/or vibration come into full contact with magnetic fiber material with corresponding reagent, complete
At the second step of extraction process, bar magnet is powered and adsorbs magnetic fiber material again；
(4) optionally, the extraction block motion is at extraction station module Gn, the bar magnet power-off, magnetism extraction material
Material is detached from bar magnet and falls into corresponding reagent bottle, and bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent,
N-th of step of extraction process is completed, bar magnet is powered and adsorbs magnetic fiber material again；
N is selected from the integer of 3-20, e.g. 3,4,5,6,8,10,12,15,18,20；
Each step includes absorption and at least once elution step at least once in the extraction process.
Preferably, each step further includes an activation step or a rinsing step in the extraction process.
It is furthermore preferred that each step includes primary activation, primary absorption, primary elution and once washes in the extraction process
De- step.
The answering in the detection of mass spectrum, chromatography or LC-MS the present invention also provides the auto extractive and purification device
With the application is the application of the auto extractive and purification device in sample pretreatment.
The auto extractive and purification device provided by the invention realize multiple types clinical sample it is one-touch it is complete from
The function of moving pre-treatment, in-line purification, automatic sampling, information source tracing, Intelligent self-checking, can effectively solve mass spectrum, chromatography is especially
LC-MS detects the problem of clinical detection field exists for a long time and bottleneck, and it is really extensive to promote advanced detection technique
Applied to clinical examination field.
Detailed description of the invention
Fig. 1 show the structure chart of extraction module
Fig. 2 show the structure chart of another optional extraction module
Fig. 3 show station function structure chart
Fig. 4 show the side block diagram of auto extractive and purification device
In attached drawing, 1- extraction module, 101- rotating device, 102- shaking device, 2- bar magnet, 201- magnetism fiber material,
3- power supply, the second guide rail of 4-, 5- limit switch, the first guide rail of 6-, 7- liquid level sensor, 8- odd-job position module, 9- extract work
Position module, 10- turntable, 1001- detent, 11- driving motor, 12- transmission mechanism, 13- store disk, 1301- reagent bottle, and 14- gives up
Waste box, 15- platform, 16- rocking equipment.
Specific embodiment
Embodiment 1 extracts module
The structure chart of the extraction module of the present embodiment is as shown in Figure 1, extraction module 1 is led including bar magnet 2, power supply 3 and second
Rail 4, bar magnet 2 are connect by circuit with power supply 3, and power supply 3 is battery.The top of bar magnet 2 is arranged in power supply 3, and the second guide rail 4 is straight
Power supply 3 is connect in succession, and power supply 3 and bar magnet 2 is driven to move up and down together.
When power supply 3 is that bar magnet 2 is powered, bar magnet 2 has magnetism, and can attract magnetic fiber material 201, magnetism extraction
Material 201 is adsorbed on the outer surface of bar magnet 2, and can be mobile with bar magnet 2.When power supply 3 stops powering, the magnetism of bar magnet 2 disappears
It loses, magnetic fiber material 201 is detached from bar magnet 2.Bar magnet 2 is cylinder, and is removably mounted on power supply 3 in the following, and protrusion electricity
3 lower surface of source.
The magnetic fiber material 201 of the present embodiment is graininess, hud typed mesoporous magnetic fiber material, to accelerate to extract
It makes even the speed of weighing apparatus, to accelerate entire extraction process；The middle part of magnetic fiber material 201 be magnetic iron oxide and silica gel, and
Micropore with 2nm, the shell of magnetic fiber material 201 are mesoporous bonded silica gel, and mesoporous with 6nm.
In use, magnetic fiber material 201 is adsorbed on the outer surface of bar magnet 2, and can when power supply 3 is that bar magnet 2 is powered
It is moved in the reagent bottle 1301 of different station module with bar magnet 2；When power supply 3 stops powering, the magnetic of bar magnet 2 disappears, magnetic
Property fiber material 201 be detached from bar magnet 2, fall into reagent bottle 1301, under stirring, magnetic fiber material 201 and reagent are molten
Liquid comes into full contact with, and completes the different step of extraction process；After magnetic fiber material 201 contacts sufficiently with reagent solution, bar magnet 2
It is once again powered up, magnetic fiber material 201 is adsorbed on bar magnet 2, and is moved to next station mould with bar magnet 2 and extraction module 1
Block.
Each extraction module 1 is equipped with a power supply 3, convenient for controlling different extraction modules 1 respectively for magnetic fiber material
201 attraction or the time not attracted, because, the corresponding different station module of difference extraction module 1, and execute extraction process not
When with step, it is desirable that magnetic fiber material 201 is different from the time that reagent comes into full contact with, for example, extraction activation, adsorb, wash
The de- time is different, and the power supply 3 of corresponding extraction module 1 can individually control an extraction module 1, so that operation is more flexible
Accurately, extraction efficiency is improved.
It extracts module 1 and is equipped with rotating device 101, rotating device 101 is connect with bar magnet 2, and bar magnet 2 can be driven to rotate, and is made
Bar magnet 2 plays the effect of stirring, and the magnetic fiber material 201 of promotion comes into full contact with reagent solution, improves extraction processing efficiency.
The upper and lower part of second guide rail 4 is equipped with limit switch 5, and limit switch 5 prompts and controls the upper limit of the movement of bar magnet 2
And lower position, it prevents bar magnet 2 and power supply 3 is more than normal moving range, destroy the station module of 1 lower section of extraction module, or
Destroy the first guide rail 6.
The lower part of the second guide rail 4 is arranged in liquid level sensor 7, and liquid level sensor 7 includes pressure sensor and capacitor
Sensor, liquid level sensor 7 detect the liquid level for the reagent bottle 1301 that bar magnet 2 protrudes into, and guarantee bar magnet 2 in each station module
Under the liquid level of place's submergence reagent bottle 1301, avoid bar magnet 2 not in contact with the reagent or bar magnet in the reagent bottle 1301 of station module
2 go deep into very much the bottom of bottle of reagent bottle 1301 and bursting reagent bottle, or mistake occur in extraction module 1 and the position of station module, and
So that bar magnet 2 and reagent bottle 1301 misplace.
Embodiment 2 extracts module
The structure chart of the extraction module of the present embodiment is as shown in Fig. 2, extraction module 1 is led including bar magnet 2, power supply 3 and second
The outside of bar magnet 2 is arranged in rail 4, power supply 3, and power supply 3 is external indoor exchange electricity consumption, the second guide rail 4 connection support bar magnet 2
Component enables the second guide rail 4 that bar magnet 2 is driven to move up and down indirectly.It extracts module 1 and is equipped with shaking device 102, and and magnetic
Stick 2 connects, and bar magnet 2 can be driven to shake, and bar magnet 2 is made to play the effect of vibration, promotes magnetic fiber material 201 and reagent solution
Come into full contact with, improve extraction processing efficiency.
The other structures of the extraction module of the present embodiment and the structure of extraction module of embodiment 1 are identical.
3 station module of embodiment
The magnetic fiber material of the present embodiment is the magnetic fiber material in embodiment 1.
The station modular structure of the present embodiment is as shown in figure 3, station module includes turntable 10, transmission mechanism 12 and storage disk
13.It stores 13 bottom hollow out of disk and side is equipped with opening, transmission mechanism 12 supports inside storage disk 13 below storage disk 13
Reagent bottle 1301, transmission mechanism 12 be conveyer belt.Transmission mechanism 12 and storage disk 13 are located at the side of turntable 10, store disk 13
The corresponding detent 1001 of opening opening.
The edge of turntable 10 is equipped with the detent 1001 of a recess, and detent 1001 has in the position at 10 edge of turntable to be opened
Mouthful, for being clamped reagent bottle 1301, the opening of the corresponding detent 1001 of the opening of storage disk 13.Station module further includes driving motor
11, the lower section of turntable 10 is arranged in driving motor 11, and turntable 10 is driven to rotate in the horizontal plane, while turntable 10 drives detent
1001 turn to different location.
Storage disk 13 is a framework only with side frame, and top and bottom hollow out is stored up when transmission mechanism 12 operates
13 positions of depositing are fixed, and only the reagent bottle 1301 in storage disk 13 is moved with transmission mechanism 12.Reagent is piled in storage disk 13
Bottle 1301, reagent bottle 1301 by transmission mechanism 12 support, and under the drive of transmission mechanism 12 to storage disk 13 opening fortune
Dynamic, the opening of storage disk 13 only allows a reagent bottle 1301 to pass through, and previous reagent bottle 1301 is in the latter reagent bottle 1301
Promotion under remove storage disk 13 opening and enter turntable 10 detent 1001, detent 1001 receive a reagent bottle 1301
Afterwards, under the drive of driving motor 11, suitable position is turned to turntable 10, waits docking extraction module 1.
In use, holding a certain amount of magnetic extraction in the reagent bottle 1301 placed inside the storage disk 13 of odd-job position module 8
Draw materials 201.
In use, reagent bottle 1301 is placed inside the storage disk 13 of extraction station module 9, according to each extraction station module
The difference of 9 extraction steps undertaken, the reagent in the reagent bottle 1301 of each extraction station module 9 is different, for example, undertaking work
Activating solution is all held in whole reagent bottles 1301 of the extraction station module 9 of change effect, undertakes the extraction station mould of suction-operated
Sample solution is all held in whole reagent bottles 1301 of block 9, undertakes whole reagent bottles of the extraction station module 9 of soil washing
Eluent is all held in 1301.
Station module is equipped with rocking equipment 16, and the lower section of driving motor 11 is arranged in rocking equipment 16, and connects driving electricity
Machine 11, and energy band turn disk 10 shakes, the magnetic fiber material 201 of promotion comes into full contact with reagent solution, improves extraction processing
Efficiency.Rocking equipment 16 has selected miniature table.
4 auto extractive of embodiment and purification device
The extraction module of the present embodiment is the extraction module of embodiment 1, and extraction station module is the extraction station of embodiment 3
Module, odd-job position module are the odd-job position module of embodiment 3.
The auto extractive of the present embodiment and the structure chart of purification device are as shown in figure 4, the setting of the first guide rail 6 is extracting module
1 top, the top of the second guide rail 4 are removably attached on the first guide rail 6, the auto extractive and purification device of the present embodiment
Including three extraction modules 1, each extraction module 1 is successively arranged sequentially on the first guide rail 6.First guide rail 6 is ring-shaped guide rail,
Each extraction module 1 does circular motion on ring-shaped guide rail, is easy to implement continuous extraction operation.
Station module is arranged on the platform 15 below extraction module 1, including an odd-job position module 8 and one group of extraction work
Position module 9, every group includes three extraction station modules 9.Odd-job position module 8 and extraction station module 9 are along the fortune of the first guide rail 6
Dynamic direction sequence is arranged and is fixed, and magnetic is convenient in 1001 position of detent of the corresponding station module turntable 10 of the bar magnet 2 of extraction module 1
Stick 2 enters in reagent bottle 1301.
The quantity of the reagent bottle 13 in storage disk 13 in odd-job position module 8 and extraction station module 9 is 38.It is magnetic
Fiber material 201 is equally placed in each reagent bottle 1301 in odd-job position module 8.
Station module further includes waste box 14, and the detent 1001 that waste box 14 is located at each station module turntable 10 is right
The lower section of position is answered, convenient for accepting the reagent bottle 1301 fallen from detent 1001.It is equipped with to push at the detent 1001 of turntable 10 and lose
Abandon the device of reagent bottle 1301.
It pushes the device for abandoning reagent bottle 1301 to be located at the lower surface of the first guide rail 6 and the position of corresponding detent 1001, is
One push rod that can be moved up and down, push rod move downward the reagent bottle 1301 that can be resisted and push down in detent 1001,
It is pushed out detent 1001, falls into the waste 14 of 10 lower section of turntable.
Extraction module 1 is moved at odd-job position module 8 by the first guide rail 6, power supply 3 and bar magnet 2 on the second guide rail 4 to
Lower movement, meanwhile, the transmission mechanism 12 of odd-job position module 8 drives the reagent bottle 1301 in storage disk 13 to harness motion, reagent
Bottle 1301 is caught in the detent 1001 of turntable 10, and turntable 10 drives reagent bottle 1301 to rotate 180 degree, and module 1 to be extracted is waited to draw；
Bar magnet 2 protrudes into reagent bottle at away from bottom end one third, and power supply 3 is powered, and bar magnet 2 draws the magnetic fiber material in reagent bottle
201, bar magnet 2 and power supply 3 move upwards later, rerun at next extraction station module 9, meanwhile, turntable continues to rotate
90 degree, reagent bottle 1301 is released detent 1001 by push rod, falls into waste box 14.
The bar magnet 2 of extraction module 1 attracts magnetic fiber material 201 at next extraction station module 9, extracts station
The reagent bottle 1301 of module enters detent 1001 and rotates to 2 lower section of bar magnet, and bar magnet 2 is moved downward and protruded into reagent bottle the bottom of away from
It holds at one third, power supply 3 powers off, and the magnetic fiber material 201 on bar magnet 2 is scattered in reagent bottle 1301, in rotating device
Under the action of 101, bar magnet 2 stirs reagent and magnetic fiber material 201 in reagent bottle 1301, meanwhile, extraction station module 9
Rocking equipment 16 drives turntable 10 and reagent bottle 1301 to shake, and the two is promoted to come into full contact with, and improves extraction efficiency.Reach balance
Afterwards, power supply 3 is powered, and the magnetic fiber material 201 in 2 adsorptive agent bottle of bar magnet moves upwards and remove reagent bottle 1301, extracts
Module 1 is moved at next extraction station module 9 by the first guide rail 6, repeats aforesaid operations, completes all of extraction process
Step.
Auto extractive and purification device further include control device, and control device includes governor circuit and control button, master control
Circuit passes through circuit connection and controls the first guide rail 6, the second guide rail 4, limit switch 5, power supply 3, transmission mechanism 12, driving motor
11 abandon the device of reagent bottle 1301 with pushing, so that all parts are run in the way of described in embodiment 1,3 and 4.Control
By key connection governor circuit, operator is facilitated to start or terminate extracting operation.
5 auto extractive of embodiment and purification device
The lower section of station module, station module is arranged in the auto extractive of the present embodiment and the first guide rail 6 of purification device
It is removably attached on the first guide rail 6, each station module is successively arranged sequentially on the first guide rail 6.At this point, the second guide rail
4 are fixed on the bracket on 6 side of the first guide rail, guarantee that the position of extraction module 1 and station module can be opposite up and down.
The auto extractive and purification device knot of the auto extractive of the present embodiment and the other structures of purification device and embodiment 4
Structure is identical.
The extraction of embodiment 6 and purification method
The auto extractive and purification device that the present embodiment uses include an odd-job position module, four extraction station modules
(G1-G4) and five extraction modules (S1-S5), such as table 1, other structures and the auto extractive of embodiment 4 and the knot of purification device
Structure is identical.
The configuration of 1 station module of table
| Odd-job position 0 | Station G1 | Station G2 | Station G3 | Station G4 |
| Bar magnet | Activating solution 1 | Sample | Leacheate 1 | Eluent 2 |
The auto extractive of the present embodiment and the application method of purification device are as follows:
(1) extraction module S1 moves to the position of odd-job position module 8 by the first guide rail 6, and bar magnet 2 is powered and draws magnetism
Fiber material 201；
(2) extraction module S1 is moved at extraction station module G1 by the first guide rail 6, and bar magnet 2 powers off, magnetism extraction material
Material 201 is scattered in corresponding reagent bottle, comes into full contact with activating solution, completes activation step, and bar magnet 2 is powered and adsorbs magnetic extraction
Draw materials 201；Meanwhile the position that module S2 moves to odd-job position module 8 is extracted, draw magnetic fiber material 201；
(3) extraction module S1 is moved at extraction station module G2 by the first guide rail 6, and bar magnet 2 powers off, magnetism extraction material
Material 201 is scattered in corresponding reagent bottle, comes into full contact with sample solution, completes adsorption step, and bar magnet 2 is powered and adsorbs magnetism
Fiber material 201；Meanwhile extracting module S2 and moving at extraction station module G1, activation step is completed, meanwhile, extract module
S3 moves to the position of odd-job position module 8, draws magnetic fiber material 201；
(4) extraction module S1 is moved at extraction station module G3 by the first guide rail 6, and bar magnet 2 powers off, magnetism extraction material
Material 201 is scattered in corresponding reagent bottle, comes into full contact with leacheate, completes rinsing step, and bar magnet 2 is powered and adsorbs magnetic extraction
Draw materials 201；Meanwhile module S2 is extracted at extraction station module G2, adsorption step is completed, meanwhile, extraction module S3 to extraction
It takes at station module G1, completes activation step, meanwhile, extraction module S4 reaches the position of odd-job position module 8, draws magnetic extraction
Material 201；
(5) extraction module S1 is moved at extraction station module G4 by the first guide rail 6, and bar magnet 2 powers off, magnetism extraction material
Material 201 is scattered in corresponding reagent bottle, comes into full contact with eluent, completes elution step, and bar magnet 2 is powered and adsorbs magnetic extraction
It draws materials 201, and the reagent bottle after elution is taken out to be detected；Meanwhile module S2 is extracted at extraction station module G3, it completes
Rinsing step, meanwhile, extraction module S3 completes adsorption step at extraction station module G2, meanwhile, extraction module S4 to extraction
At station module G1, activation step is completed, while extracting the position that module S5 reaches odd-job position module 8, draws magnetic extraction material
Material 201；
(7) extraction module S1 power-off is recycled used magnetic fiber material 201, is then transported again by the first guide rail 6
The position of odd-job position module 8 is moved, magnetic fiber material 201 is drawn；Meanwhile module S2 is extracted at extraction station module G4,
Complete elution step；Meanwhile module S3 is extracted at extraction station module G3, rinsing step is completed, meanwhile, extraction module S4 is arrived
It extracts at station module G2, completes sample absorption；Meanwhile module S5 is extracted at extraction station module G1, complete activation step；
And so on, above-mentioned steps are recycled, all samples at extraction station module G2, which extract, to be completed.
Claims (13)
1. a kind of extraction and purification method based on magnetic fiber material, which is characterized in that the extraction and purification method include
Following steps:
(1) bar magnet is powered and draws magnetic fiber material；
(2) bar magnet is moved into the place extraction station module G1, bar magnet power-off, magnetic fiber material disengaging bar magnet is simultaneously fallen into pair
In the container answered, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, first for completing extraction process
Step, bar magnet are powered and adsorb magnetic fiber material again；Or bar magnet is in energized state, turns in a reservoir with magnetic material
It is dynamic, complete first step of extraction process；
(3) bar magnet moves at extraction station module G2, and the bar magnet power-off, magnetic fiber material is detached from bar magnet and falls into correspondence
Container in, bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, complete second of extraction process and walk
Suddenly, bar magnet is powered and adsorbs magnetic fiber material again；
(4) optionally, the bar magnet moves at extraction station module Gn, and the bar magnet power-off, magnetic fiber material is detached from magnetic
Stick is simultaneously fallen into corresponding container, and bar magnet rotation and/or vibration come into full contact with magnetic fiber material with reagent, and completion extracted
N-th of step of journey, bar magnet are powered and adsorb magnetic fiber material again；
N is selected from the integer of 3-20, e.g. 3,4,5,6,8,10,12,15,18,20；
Each step includes absorption and at least once elution step at least once in the extraction process.
2. the extraction and purification method described in claim 1 based on magnetic fiber material, which is characterized in that the extraction
Each step further includes an activation step or a rinsing step in journey.
3. the extraction and purification method as claimed in claim 2 based on magnetic fiber material, which is characterized in that the extraction
Each step includes the steps that once activation, primary absorption, primary elution and once elution in journey.
4. a kind of auto extractive and purification device based on magnetic fiber material, which is characterized in that the auto extractive and purification
Device includes at least one extraction module, at least three station modules and the first guide rail, the extraction module or station module energy
Moved on the first guide rail, the lower section of extraction module is arranged in the station module, and including at least one odd-job position module and
At least two extraction station modules, station module are arranged along the direction of motion sequence of the first guide rail, and magnetic is equipped in station module
Property fiber material；
The extraction module includes bar magnet, power supply and the second guide rail, and the bar magnet connects power supply, and can be on the second guide rail up and down
Movement, absorption and disengaging of the power supply control bar magnet to the magnetic fiber material.
5. auto extractive according to claim 4 and purification device, which is characterized in that the bar magnet is cylinder, described
Bar magnet protrudes the lower surface of the extraction module, and can extend into the reagent bottle of the station module.
6. auto extractive according to claim 4 and purification device, which is characterized in that the magnetism fiber material is particle
Shape material, magnetic fiber material are selected from non-hud typed conventional magnetic fiber material and hud typed mesoporous magnetic fiber material；
The middle part of the hud typed mesoporous magnetic fiber material is inorganic material or high polymer material solid or with micropore, described
The average pore size of micropore is less than 3nm；The shell cladding of the hud typed mesoporous magnetic fiber material have it is mesoporous inorganic or
High polymer material, the mesoporous average pore size are 3-50nm；
The magnetism fiber material is placed in the reagent bottle in the odd-job position module.
7. auto extractive according to claim 4 and purification device, which is characterized in that the upper of bar magnet is arranged in the power supply
Side, second guide rail is directly connected to power supply, and power supply and bar magnet is driven to move up and down together.
Preferably, each extraction module is equipped with a power supply.
8. auto extractive according to claim 4 and purification device, which is characterized in that the extraction module is equipped with rotation dress
It sets or one or both of shaking device, the rotating device and/or shaking device are connect with the bar magnet, and can drive magnetic
Stick rotation and/or vibration.
9. auto extractive according to claim 4 and purification device, which is characterized in that the position of the first guide rail setting
The lower section of top or station module selected from the extraction module, the extraction module or station module connection described first are led
Rail, and can be moved on the first guide rail.
10. auto extractive according to claim 4 and purification device, which is characterized in that the station module include turntable,
Transmission mechanism and storage disk, the storage pan bottom hollow out and side are equipped with opening, and the transmission mechanism is below storage disk, institute
The edge for stating turntable is equipped with the detent of at least one recess, the detent of the corresponding turntable of opening of the storage disk.
Preferably, the station module further includes driving motor, and the driving motor drives the turntable to rotate in the horizontal plane.
11. auto extractive according to claim 10 and purification device, which is characterized in that the station module is equipped with concussion
Device, the rocking equipment connects the turntable or driving motor, and energy band turn disk shakes.
12. auto extractive according to claim 4 and purification device, which is characterized in that the auto extractive and purification dress
Setting further includes control device, and the control device includes governor circuit and control button, and the governor circuit passes through circuit connection
And first guide rail, the second guide rail, power supply, transmission mechanism and driving motor are controlled, the control button connects governor circuit,
Operator is facilitated to operate the control device.
13. the application of auto extractive described in claim 1 and purification device in the detection of mass spectrum, chromatography or LC-MS,
It is characterized in that, the application is the application of the auto extractive and purification device in sample pretreatment.
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| CN111330555A (en) * | 2020-03-13 | 2020-06-26 | 天津迪沃特生物电子科技有限公司 | Magnetic core-shell mesoporous silica gel material and preparation method and application thereof |
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