CN105527150A - High-throughput magnetic extraction enrichment device and enrichment method - Google Patents
High-throughput magnetic extraction enrichment device and enrichment method Download PDFInfo
- Publication number
- CN105527150A CN105527150A CN201510915818.0A CN201510915818A CN105527150A CN 105527150 A CN105527150 A CN 105527150A CN 201510915818 A CN201510915818 A CN 201510915818A CN 105527150 A CN105527150 A CN 105527150A
- Authority
- CN
- China
- Prior art keywords
- enrichment
- magnetic
- container
- support bar
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
Abstract
The invention belongs to the field of a magnetic extraction device and discloses a high-throughput magnetic extraction enrichment device and an enrichment method. The device comprises magnetic adjustable enrichment probes, an enrichment probe support, a support bar, a container tray and a pedestal, and is characterized in that several enrichment probes are fixed on the enrichment probe support, which is connected to the support bar; the support bar is connected to the pedestal; the container tray is positioned below the enrichment probes; container placement sites which are corresponding to the enrichment probes are arranged on the container tray; the container tray is connected to the support bar and the pedestal, or the container tray is placed on the pedestal or a tray support, which is connected to the support bar or the pedestal; and one or more adjusting devices which enable the enrichment probes and the container tray to carry out relative motion are arranged on the enrichment probe support, the tray support, the container tray, the support bar and/or the pedestal. The device has a simple structure, is low-cost, is flexible to move, is simple to operate, and can remarkably enhance sample treatment efficiency. The method is simple and efficient.
Description
Technical field
The invention belongs to magnetic extraction equipment field, relate to a kind of high flux magnetic extracting and enriching device and enrichment method, specifically, relate to a kind of in magnetic dispersive solid-phase extraction sample pretreatment process, a kind of high flux magnetic extracting and enriching device of magnetic extraction particle in energy enrichment, transfer and separation solution.
Background technology
Magnetic dispersive solid-phase extraction (MagneticDispersiveSolid-PhaseExtraction, MDSPE) technology is unique, the eco-friendly Sample Pretreatment Technique risen in the last few years, is applied magnetic or the magnetisable nano material a kind of dispersive solid-phase extraction technology as adsorbing base.Different from traditional Solid-Phase Extraction (SPE) technology, this nano particle can be exposed to system to be measured completely, and fully contact with it, can adsorb from large volume system to be measured at short notice and extraction test substance, this technology can realize being separated by means of only applying external magnetic field, simple to operate, save time fast, without the need to troublesome operation such as centrifugal filtrations.Thus this technology is at cell separation, drug transport, enzyme immobilizatio, all show high application prospect in the numerous areas such as absorption-separation, environmental science, Food Science, genomics of target organic.
The main process of MDSPE technology for first magnetic-particle adsorbent is added in sample solution or suspending liquid, ultrasonic disperse, determinand extracts completely in adsorbent, assembles adsorbent, outwell solution in the additional attraction of container; Remove magnet, add clean-out system, ultrasonic disperse, repeat magnetic separation step, outwell clean-out system, then add small size eluant, eluent determinand is eluted from magnetic material.
At present in this process, the following problem of ubiquity:
(1), when needing process batch samples solution, require great effort consuming time, can not carry out, processing flux is low simultaneously;
(2) due to the absorption of chamber wall, during pouring soln, thoroughly, totally, next step operation can not be affected, when particularly liquor capacity is little;
(3) when testing concentration in sample solution is extremely low, large volume extraction need be carried out, along with container dimensional increases, in the middle part of additional magnet distance container, solution becomes far away, magnetic force weakens, and in the middle part of container, magnetic material is separated magnetic force deficiency, and disengaging time extends, absorption not exclusively, thus causes the problems such as the recovery is low, poor reproducibility, processing time length.
Summary of the invention
Object of the present invention is in order to solve the problems such as existing magnetic material extraction and enrichment method treatment step is loaded down with trivial details, consuming time, a kind of high flux magnetic extracting and enriching device is provided, to be applied in enormous quantities and bulk sample extraction rapidly and efficiently catch magnetic material, and conveniently realize transfer, the separation of magnetic material, improve flux and the efficiency of sample preparation.
A further object of the invention is to provide the enrichment method adopting above-mentioned enriching apparatus.
The object of the invention is to be realized by following technical proposal:
A kind of high flux magnetic extracting and enriching device, comprises the adjustable enrichment probe of magnetic, container tray, enrichment probe bracket, support bar, base; Wherein some enrichment probes are fixed on enrichment probe bracket, and enrichment probe bracket is connected with support bar, and support bar is connected with base, and container tray is positioned at enrichment probe below, and container tray arranges the container deposit position of popping one's head in corresponding with enrichment; Container tray is connected with support bar or base, or container tray is positioned on base or tray supporter, and tray supporter is connected with support bar or base; Enrichment probe bracket, tray supporter, container tray, support bar and/or base are provided with one or more regulating device making enrichment probe and container tray do relative motion.
Described enriching apparatus, wherein the adjustable enrichment probe of magnetic is adopt electromagnetism regulate the enrichment probe of magnetic or adopt the enrichment probe of permanent magnet mechanical adjustment magnetic.
Described enriching apparatus, wherein adopt electromagnetism regulate the enrichment of magnetic probe comprise electromagnet, electromagnet fixed bar, for regulating magnet magnetic strong and weak or with presence or absence of electromagnetic control device; Electromagnet fixed bar one end fixed electromagnet, the other end is fixed on enrichment probe bracket.In order to avoid forever absorption and long-term corrosion, electromagnet can adopt insulation, anticorrosion, waterproof, not easily Magnetized Material parcel or encapsulate, such as plastic or other material.Electromagnet hold-down bars can adopt smooth surface to make without material such as the polytetrafluoroethylmaterial material of adsorbability.
Electromagnetic control device can be arranged on any position not affecting enrichment procedure of enriching apparatus, is such as arranged on enrichment probe bracket, support bar and/or base.The electromagnet that electromagnetic control device can be popped one's head in all enrichments carries out adjusted in concert, also can carry out independent regulation by single enrichment probe, realize regulating respectively each enrichment probe as by program control device or each enrichment probe all arranges an electromagnetic control device.When gross sample carries out high flux enrichment under normal circumstances, adjusted in concert carried out to whole probes just passable, if but perhaps carry out independent adjustment to the magnetic of certain probe when needing to arrange contrast and just needed.
Described enriching apparatus, wherein adopt the enrichment of permanent magnet mechanical adjustment magnetic to pop one's head in the permanent magnet, the outer tube that comprise interior rod, be connected with interior excellent lower end, outer sleeve upper open lower end is cecum, and permanent magnet can move up and down in outer tube with interior rod; Enrichment probe bracket is divided into two layers, upper layer bracket is connected with interior excellent top, lower floor support is connected with outer tube top, and upper and lower layer support is connected with support bar respectively, and support and/or support bar are provided with one or more regulating device making levels support do relative motion.
Further, ring baffle can be set at the middle and lower part outside surface of outer tube.The position of annular plate washer preferably adopts adjustable, position is determined according to concrete solution level, or the usual requirement of liquid level of solution is immersed according to outer tube lower end, in position be fixedly installed annular plate washer, such as can be arranged on the position that outer tube lower end is closed on sample solution liquid level and immersed below elute soln liquid level.The object arranging ring baffle is owing to a small amount of magnetic-particle being adsorbed on outer tube sidewall can being made when permanent magnet frequently moves up upwards slightly to move to more than elute soln liquid level, causing this part magnetic-particle to elute and to produce error.
Preferably, described interior rod can adopt plastic material to make, and in order to avoid forever adsorbing and long-term corrosion, outer tube can adopt smooth surface to make without material such as the polytetrafluoroethylmaterial material of adsorbability.
Described enriching apparatus, wherein container deposit position is container deposit groove or container deposit hole.
Described enriching apparatus, wherein container tray and tray supporter, support bar or base connected mode are that buckle-type is connected.Adopt buckle-type to connect and can realize container tray replacing easily, integral replacing can be carried out to the container on container tray like this, improve container and change efficiency.If container tray is directly placed on base or tray supporter, preferably there is the position limiting structure of form fit, prevent container tray because of reason displacements such as vibrations.Certainly, also can not change container tray, but adopt other modes to carry out integral replacing to container, such as adopt fixture or the mechanical arm that once can capture several containers, but obvious like this container tray of directly not changing comes convenient and swift.
Container comprises the sampling receptacle filled containing the sample solution treating rich magnetic material (as magnetic-particle), or fills the elution container of the elute soln got off by rich magnetic material elutes.
Described enriching apparatus, the regulating device wherein making enrichment probe and container tray do relative motion comprises:
The regulating device that enrichment probe bracket is moved up and down that enrichment probe bracket or support bar are arranged; And/or, the regulating device that container tray is moved up and down that container tray or support bar or base are arranged.
The enrichment probe bracket that makes that enrichment probe bracket or support bar are arranged makes the regulating device of to-and-fro movement by a small margin, circular motion or significantly translation motion; And/or the container tray that makes arranged on container tray or support bar or base makes the regulating device of to-and-fro movement by a small margin, circular motion or significantly translation motion.
The effect moved up and down can make enrichment probe move down in insertion solution and move up on the one hand and leave container, such as insert in sampling receptacle and contact with sample solution, rich magnetic particle can be treated in adsorption sample solution, or insert in eluant, eluent by by the magnetic-particle wash-out of enrichment probe absorption in elute soln.Realize such function also container tray to be allowed to move up and down realize by allowing enrichment pop one's head in motionless, or enrichment probe and container tray all move up and down to realize, no matter which parts moves up and down, and inserts solution as long as can realize enrichment probe and leaves container.Another effect moved up and down makes to be formed between enrichment probe and container tray the space of certain altitude to be convenient for changing container tray.
To-and-fro movement by a small margin, the effect of circular motion mainly makes enrichment pop one's head in and has the effect of stirring rod concurrently, just can be popped one's head in by enrichment after under the cooperation moved up and down, enrichment probe inserts solution reciprocating, circular motion realizes the beating action to solution, as mentioned above, the to-and-fro movement of enrichment probe, circular motion both can by the to-and-fro movement by a small margin of enrichment probe bracket do, circular motion drives enrichment probe reciprocating, circular motion realizes, also can only by the to-and-fro movement by a small margin of container tray do, circular motion drives container reciprocating, circular motion thus beating action is played in the relative motion that enrichment is popped one's head in, or enrichment probe bracket and container tray all do to-and-fro movement by a small margin, circular motion, no matter which parts is reciprocating, circular motion, as long as enrichment probe can be realized to be played agitating solution effect and both can.In addition, described here is do not encounter container inner wall with enrichment probe to be advisable by a small margin.
Significantly the effect of translation motion mainly provides space for convenience of changing container tray.Enrichment probe bracket shifts out the surface level projection in zone that the surface level projection in zone of container tray or container tray shift out enrichment probe bracket or enrichment probe, or enrichment probe bracket and container tray mutually movement make that their surface level projection in zone is not overlapping or major part is not overlapping, replacing container tray is made to have enough spaces to be convenient for changing, also avoid touching enrichment probe or miscellaneous part, prevent from causing enrichment tip damage or solution to pour down or forth.Enrichment probe bracket or container tray are done significantly translation motion cooperation and are moved up and down and can provide more operating space for changing container tray.
Certainly, it is optional for replacing container tray that enrichment probe bracket or container tray do significantly translation motion, such as, as long as make enrichment pop one's head in lower end higher than edge on container, container tray level is extracted out or pushes and can complete container tray replacing, at this time perhaps do not need special to arrange the device making container tray significantly translation, but the stable replacing that horizontal guide rail is obviously more conducive to container tray is set.
The replacing of described container tray not only refers to the replacing of container tray itself, also comprise and be placed on some sampling receptacles filling solution on container tray or elution container, the i.e. integral replacing of container tray and container (solution), because this instrument for mainly batch samples solution in the enrichment of magnetisable material, integral replacing could improve bioaccumulation efficiency, realizes high-throughout batch processing object.
Described enriching apparatus, the regulating device wherein making levels support do relative motion comprises:
Upper layer bracket or support bar arrange the regulating device that upper layer bracket is moved up and down; And/or, lower floor support or support bar arrange the regulating device that lower floor support is moved up and down;
Upper layer bracket or support bar are arranged and makes the to-and-fro movement by a small margin of upper layer bracket do, the regulating device of circular motion; And/or, lower floor support or support bar are arranged and makes the to-and-fro movement by a small margin of lower floor support do, the regulating device of circular motion.
The effect that levels support moves up and down is the magnetic for mechanical adjustment outer tube lower end, when upper layer bracket moves down and/or lower floor support moves, the permanent magnet that interior excellent lower end is connected is inserted into outer tube lower end, outer tube lower end magnetic enhancement, can will treat that rich magnetic species adsorbs is on outer tube lower end outer wall, realizes the enrichment of magnetisable material in sample solution; When upper layer bracket to be moved and/or lower floor support moves down, the permanent magnet that interior excellent lower end is connected detaches outer tube lower end, and outer tube lower end magnetic weakens or loses, and the magnetisable material being enriched in outer tube lower end outer wall just splits away off or is easy to by elute soln wash-out.
The effect of the to-and-fro movement by a small margin of upper layer bracket and/or lower floor support, circular motion enrichment is popped one's head in have the effect of stirring rod concurrently, in this case, the connection of the connection of upper layer bracket and interior rod and lower floor support and outer tube preferably adopts and flexibly connects, to make interior rod and outer tube motion flexibly and to avoid damaging.If levels support only moves up and down and not reciprocating, circular motion, then the connection of upper layer bracket and interior rod and the connection of lower floor support and outer tube can adopt and be rigidly connected.
Described enriching apparatus, wherein to move up and down regulating device be swing pinion with band tooth track combination, screw and rotating wire shoulder combine, position limiting slide block and track combination or pilot pin and pilot hole combine; The regulating device of to-and-fro movement, circular motion is push cam mechanism, toggle, back and forth screw mechanism or fan-shaped rotary reciprocator, and these devices also can realize circular motion and to-and-fro movement is changed mutually; Significantly the regulating device of translation motion is guide rail, turning axle or curved bar.In fact, the regulating device realizing above-mentioned forms of motion has a lot of type in the prior art, all may be used for this equipment.These regulating devices both can be manually may also be electronic.Further, program control device can also be set condensation control is carried out to each regulating device, to regulate the amplitude, speed, duration, order, interval time etc. of each type games, thus realize automation function.
To-and-fro movement, circular motion make the track of enrichment probe lower end show as linear reciprocation, circular motion, fan-shaped swing etc., movement locus is mainly at surface level, but there is the situation at pitch angle in the movement locus part due to enrichment probe, so neither proper surface level.The enrichment probe of such as levels support reciprocating drive permanent magnet mechanical adjustment magnetic plays the situation of beating action.
Support bar can be one or more as required, can be arranged on the side of base or relative both sides, also can be arranged on centre, to realize supporting and to keep balance, and does not affect operation.
Present invention also offers the adjustable enrichment probe of a kind of magnetic, this enrichment is popped one's head in the permanent magnet, the outer tube that comprise interior rod, be connected with interior excellent lower end, outer sleeve upper open lower end is cecum, and interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod.
Described enrichment probe, wherein outer tube middle and lower part outside surface is also provided with annular plate washer.
Described enrichment probe, wherein the position of annular plate washer is adjustable.
Described enrichment probe, rod and/or permanent magnet xsect ovalize wherein, the cross-section of pipeline ovalize of outer tube, interior rod and/or permanent magnet and outer tube rotate to an angle can block fixing.
Described enrichment probe, wherein, rod and/or outer tube are also provided with and make interior rod and/or permanent magnet in position keep stationary device.This device comprises buckle structure, movable slider and guide rail structure, silk shoulders screw structure, ratchet pawl structures etc.By these structures, interior rod can be made to move in outer tube thus drive permanent magnet to keeping fixing during appropriate location.
Have and make interior rod and/or permanent magnet and outer tube in position keep the enrichment of fixed function to pop one's head in both can to use in above-mentioned enriching apparatus, also can be used alone.Under the situations such as, field use little in quantity of sample handling, adopt separately such probe just to have obvious advantage, easy, in time, cost is low.
Beneficial effect of the present invention:
Compared with prior art, the present invention has following advantages:
The present invention is specially for the enriching apparatus of magnetic material extraction design, the complex steps of traditional magnetic extract and separate and enrichment can be simplified, can the magnetic material disperseed in extraction solution be trapped completely on enrichment probe efficiently rapidly, realize enrichment, transfer and be separated, without the need at the additional magnet of container, easy and simple to handle, without the need to pouring soln in former container, not by the impact of dissolvent residual, when overcoming large volume extraction, magnetic fiber material can not reclaim problem completely.Compared with conventional manual method pre-service, extract while carrying out batch samples, improve the flux of sample preparation, better accuracy and reappearance can be reached simultaneously, significantly improve sample preparation efficiency, be particularly suitable for the extracting and enriching of magnetic material in bulk sample in enormous quantities.Enrichment probe magnetic of the present invention is adjustable, the solution of different volumes in energy container handling.
In addition, this device is simply portable, can realize automation mechanized operation, and the site disposal of sample is carried out at scene of can directly taking to, enrichment concentrates, and avoids a large amount of sample and not easily transports and rotten in transportation and polluting.Relative to traditional magnetic dispersive solid-phase extraction (MDSPE) method, this apparatus structure is simple, with low cost, flexibly mobile, easy and simple to handle, the efficiency of sample preparation can be significantly improved, both saved the time, again reduced cost, the nonstandard interference of manual operation can also have been got rid of, and then improve the reliability of subsequent analysis result, can be applicable to the various sample preparation field needing magnetic resolution.The enrichment method adopting apparatus of the present invention to carry out is simple, efficient.
The enrichment probe adjustable magnetic of mechanical adjustment magnetic provided by the invention is strong and weak, and can reduce power consumption, economize energy, adopts the enrichment of annular plate washer to pop one's head in and can make by the abundant wash-out of magnetisable material adsorbed, reduce error.
Accompanying drawing explanation
Fig. 1 is the structural representation of an embodiment of the enriching apparatus adopting electromagnetism to regulate.
Fig. 2 is the structural representation of another embodiment of the enriching apparatus adopting electromagnetism to regulate.
Fig. 3 is the structural representation of an embodiment of the enriching apparatus adopting mechanical adjustment magnetic.
Fig. 4 is the structural representation of another embodiment of the enriching apparatus adopting mechanical adjustment magnetic.
Fig. 5 adopts mechanical adjustment magnetic and support bar at the structural representation (solid) of the embodiment of an enriching apparatus of centre.
Fig. 6 is the structural representation (section) of enriching apparatus described in Fig. 5.
Fig. 7 is the structural representation (solid) of the container tray in enriching apparatus described in Fig. 5.
Fig. 8 is the structural representation (section) of an embodiment of the enrichment probe adopting mechanical adjustment magnetic.
Fig. 9 is the structural representation (section) of another embodiment of the enrichment probe adopting mechanical adjustment magnetic.
Figure 10 is when adopting the interior rod of the enrichment of mechanical adjustment magnetic probe and outer tube not to block and the view (xsect) in the time of blocking.
Figure 11 is the structural representation (section) adopting the interior rod of the enrichment of mechanical adjustment magnetic probe and outer tube to adopt screw-nut structure spacing.
Figure 12 is the structural representation (section) adopting the interior rod of the enrichment of mechanical adjustment magnetic probe and outer tube to adopt ratchet pawl structures spacing.
In above-mentioned figure, 1, base; 2, container tray; 3, enrichment probe lower floor support; 4, enrichment probe upper layer bracket; 5, support bar; 6, container tray adjusting knob; 7, enrichment probe lower floor support adjusting knob; 8, enrichment probe upper layer bracket adjusting knob; 9, interior rod; 10, outer tube; 11, permanent magnet; 12, container deposit position; 13, outer tube mounting hole; 14, interior excellent mounting hole; 15, to-and-fro movement regulating device; 16, upper/lower guide; 17, tray supporter; 18, horizontal guide rail; 19, program control device; 20, buckle; 21, container; 22, ring baffle; 23, electromagnetism regulates the enrichment control head of magnetic; 24, electromagnet; 25, electromagnetic control device; 26, enrichment probe bracket; 27, enrichment probe bracket adjusting knob; 28, connecting link; 29, electromagnet fixed bar; 30, tray openings through slot; 31, leading screw; 32, screw; 33, ratchet; 34, ratchet.
Embodiment
Below in conjunction with accompanying drawing, specific embodiments of the invention are described in detail.
The present embodiment is in order to simplify traditional magnetic dispersive solid-phase extraction (MDSPE) process, accelerate the speed of magnetic material extracting and enriching, improve flux and the efficiency of sample preparation, realize the batch processing of sample, propose a kind of high flux magnetic extracting and enriching device.
Embodiment 1
As shown in Figure 1, a kind of high flux magnetic extracting and enriching device, comprises the enrichment probe 23, enrichment probe bracket 26, support bar 5, container tray 2, the base 1 that adopt electromagnetism to regulate magnetic; Wherein some enrichment probes 23 are fixed on enrichment probe bracket 26, enrichment probe bracket 26 is flexibly connected with support bar 5, by the regulating device 27 be arranged on support bar, enrichment probe bracket is moved up and down along support bar 5, support bar 5 is fixedly connected with base 1, container tray 2 is positioned at below enrichment probe 23, container tray 2 arranges the container deposit position 12 of popping one's head in corresponding with enrichment, container deposit position is container deposit groove or container deposit hole, container installation position can be placed the container 21 of the container filling testing sample or eluent; Container tray can directly be positioned on base, or container tray adopts buckle-type to be connected with base by connecting link 28, connecting link 28 can double as and regulate container tray to make reciprocating vibration rod by a small margin, container tray can be driven to do to-and-fro movement by a small margin under motor (omitting in figure) drives, indirectly make enrichment pop one's head in and play stirring rod effect.Enrichment probe bracket 26 moves down and enrichment probe lower end can be driven to immerse solution, there is electromagnet 24 enrichment probe lower end, the magnetic of electromagnet with or without or power regulated by electromagnetic control device 25, enrichment probe bracket 26 moves up and enrichment can be driven to pop one's head in lower end higher than edge on container to be convenient for changing container tray 2, press buckle 20 container tray to be taken off from base, container tray and container 21 (comprising the solution filled) integral replacing can be adopted when changing container tray.
Electromagnetism regulate the enrichment of magnetic probe 23 comprise electromagnet 24, electromagnet fixed bar 29, for regulating magnet magnetic strong and weak or with presence or absence of electromagnetic control device 25; Electromagnet fixed bar 29 one end fixed electromagnet 24, the other end is fixed on enrichment probe bracket 26.In order to avoid forever absorption and long-term corrosion, electromagnet can adopt insulation, anticorrosion, waterproof, not easily Magnetized Material parcel or encapsulate, such as plastic or other material.Electromagnet hold-down bars can adopt ganoid polytetrafluoroethylmaterial material to make.
Embodiment 2
As shown in Figure 2, a kind of high flux magnetic extracting and enriching device, support bar is 2, be arranged on base both sides, more advantageous installation is stablized, and be separately provided with presetting apparatus 19, presetting apparatus carries out Comprehensive Control to each regulating device, to regulate the amplitude, speed, duration, order, interval time etc. of each type games, thus realize automation function.All the other are with embodiment 1.
Embodiment 3
As shown in Figure 3, a kind of high flux magnetic extracting and enriching device, comprise the enrichment probe, enrichment probe bracket, support bar, container tray, the base that adopt permanent magnet mechanical adjustment magnetic, the enrichment of permanent magnet mechanical adjustment magnetic probe is wherein adopted to comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, and permanent magnet can move up and down in outer tube with interior rod; Enrichment probe bracket is divided into two layers, upper layer bracket 4 is connected with interior excellent top, lower floor support 3 is connected with outer tube top, upper and lower layer support is connected with support bar 5 respectively, support bar is respectively equipped with the adjusting knob 8 that upper layer bracket is moved up and down and the adjusting knob 7 that lower floor support is moved up and down, adjusting knob 8 and 7 drive respectively levels support vertically guide rail 16 move.What the intercropping of levels support was relative can make permanent magnet move up and down in outer tube when moving up and down, its lower end is as enrichment positions in addition, permanent magnet is when outer tube lower end, enrichment positions magnetic enhancement, can will treat that rich magnetic species adsorbs is on outer tube lower end outer wall, realizes the enrichment of magnetisable material in sample solution; When permanent magnet is away from outer tube lower end, enrichment positions magnetic weakens or loses, and the magnetisable material being enriched in outer tube lower end outer wall just splits away off or is easy to by elute soln wash-out.When levels support entirety moves up and down, enrichment can be made to pop one's head in and to stretch in appearance liquid or leave container.
Arrange in support bar bottom and drive support bar to do reciprocating regulating device 15 (as reciprocating motor) by a small margin, realize with support bar being that rotation axis drives levels support entirety to make sector reciprocating motion, indirectly make enrichment pop one's head in and make sector reciprocating motion by a small margin, thus make to stretch into enrichment probe in solution and play the effect of stirring rod.
Container tray 2 is positioned on tray supporter 17, and adopt snap to fix, tray supporter 17 is connected with base 1.The container of pressing buckle 20 exchangeable reservoir pallet and upper placement thereof, directly can take off or card upper container pallet, or make container tray 2 extract out easily along the horizontal guide rail 18 on tray supporter 17 or push.
The middle and lower part outside surface of outer tube arranges ring baffle 22.The position of annular plate washer adopts adjustable, position is determined according to concrete solution level, object to make a small amount of magnetic-particle being adsorbed on outer tube sidewall upwards slightly move to more than elute soln liquid level when preventing permanent magnet from frequently moving up, and causes this part magnetic-particle to elute and to produce error.
Base 1 is provided with presetting apparatus 19, and presetting apparatus carries out Comprehensive Control to each regulating device, to regulate the amplitude, speed, duration, order, interval time etc. of each type games, thus realizes automation function.
Embodiment 4
As shown in Figure 4, as different from Example 3, tray supporter 17 is connected with support bar 5, upper/lower guide adjusting tray support is coordinated to move up and down along support bar 5 by adjusting knob 6, arrange in support bar bottom and drive support bar to do reciprocating regulating device 15 (as reciprocal screw mechanism) by a small margin, driving tray supporting band moves container tray and does to-and-fro movement by a small margin, thus relatively also can realize the effect that enrichment probe plays stirring rod.In addition, outer tube middle and lower part outside surface does not establish ring baffle 22, and other are with embodiment 3.
Embodiment 5
As shown in Fig. 5,6,7, a kind of high flux magnetic extracting and enriching device, comprise the enrichment probe, enrichment probe bracket, support bar, container tray, the base that adopt permanent magnet mechanical adjustment magnetic, the enrichment of permanent magnet mechanical adjustment magnetic probe is wherein adopted to comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, and permanent magnet can move up and down in outer tube with interior rod; Enrichment probe bracket is divided into two layers, upper layer bracket 4 is flexibly connected with interior excellent 9 tops by interior excellent mounting hole 14, lower floor support 3 is flexibly connected by outer tube mounting hole 13 and outer tube 10 top, upper and lower layer support is connected with support bar 5 respectively, support bar is respectively equipped with the adjusting knob 8 that upper layer bracket is moved up and down and the adjusting knob 7 that lower floor support is moved up and down, and adjusting knob 8 and 7 drives levels support to move up and down (screw thread matched with each adjusting knob is located on support bar) along support bar respectively.What the intercropping of levels support was relative can make permanent magnet move up and down in outer tube when moving up and down, its lower end is as enrichment positions in addition, permanent magnet is when outer tube lower end, enrichment positions magnetic enhancement, can will treat that rich magnetic species adsorbs is on outer tube lower end outer wall, realizes the enrichment of magnetisable material in sample solution; When permanent magnet is away from outer tube lower end, enrichment positions magnetic weakens or loses, and the magnetisable material being enriched in outer tube lower end outer wall just splits away off or is easy to by elute soln wash-out.When levels support entirety moves up and down, enrichment can be made to pop one's head in and to stretch in appearance liquid or leave container.
Container tray 2 is positioned at enrichment probe below, container tray 2 arranges the container deposit position 12 of popping one's head in corresponding with enrichment, container deposit position is container deposit groove or container deposit hole, container installation position 12 can be placed and fill the container of testing sample or the container of eluent.
Arrange in the middle part of support bar and drive lower floor support 3 to do reciprocating regulating device 15 (as reciprocating motor) by a small margin, realizing lower floor support 3 is that sector reciprocating motion is made in axle center with support bar, drive simultaneously enrichment probe above in excellent mounting hole 14 swing back and forth for suspension point certain amplitude, thus make to stretch into enrichment in solution and pop one's head in and play the effect of stirring rod.
Because support bar 5 is arranged on centre, container tray 2 can arrange the opening through slot 30 that is arrived at tray center, the impact not by support bar 5 when making container tray 2 extract out or push.
Embodiment 6
As shown in Figure 8, a kind of high flux magnetic extracting and enriching probe, comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod; Interior rod and permanent magnet shape of cross section are not limit, and the pipe shape of outer tube does not affect moving up and down of interior rod and permanent magnet.
Embodiment 7
As shown in Figure 9, a kind of high flux magnetic extracting and enriching probe, comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod; Interior rod and permanent magnet shape of cross section are not limit, and the pipe shape of outer tube does not affect moving up and down of interior rod and permanent magnet.Annular plate washer 22 is also provided with at outer tube middle and lower part outside surface.The position of annular plate washer adopts adjustable, position is determined according to concrete solution level, object to make a small amount of magnetic-particle being adsorbed on outer tube sidewall upwards slightly move to more than elute soln liquid level when preventing permanent magnet from frequently moving up, and causes this part magnetic-particle to elute and to produce error.
Embodiment 8
If Fig. 8 is in conjunction with shown in Figure 10 or if Fig. 9 is in conjunction with shown in Figure 10, a kind of high flux magnetic extracting and enriching probe, comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod; Interior rod and/or permanent magnet xsect ovalize, the cross-section of pipeline ovalize of outer tube, interior rod and/or permanent magnet and outer tube rotate to an angle just can block fixing.Outer tube middle and lower part outside surface can arrange or not establish annular plate washer 22.
Embodiment 9
As shown in figure 11, a kind of high flux magnetic extracting and enriching probe, comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod; Interior rod and permanent magnet shape of cross section are not limit, and the pipe shape of outer tube does not affect moving up and down and rotating of interior rod and permanent magnet.Interior rod is provided with leading screw 31, and outer tube middle and upper part is provided with the screw 32 mated with leading screw, and screw position is fixed or adjustable, rod in rotating, and interior rod can be made to move up and down and drive permanent magnet 11 to desired location, stop operating and can remain on this position under screw-nut effect.This enrichment probe both can use in above-mentioned enriching apparatus, also can be used alone.
Embodiment 10
As shown in figure 12, a kind of high flux magnetic extracting and enriching probe, comprise interior excellent 9, the permanent magnet 11, the outer tube 10 that are connected with interior excellent lower end, outer sleeve upper open lower end is cecum, interior excellent upper end exceeds outer sleeve upper, and permanent magnet can move up and down in outer tube with interior rod; Interior rod and permanent magnet shape of cross section are not limit, and the pipe shape of outer tube does not affect moving up and down of interior rod and permanent magnet.Interior rod is provided with ratchet 33, and outer tube middle and upper part is provided with the ratchet 34 mated with ratchet, and ratchet can elastic reset, pushes away, draws interior rod, interior rod can be made to move up and down and drive permanent magnet 11 to desired location, stops push-and-pull can remaining on this position under the effect of ratchet.This enrichment probe both can use in above-mentioned enriching apparatus, also can be used alone.
Embodiment 11
Use apparatus of the present invention to carry out the method for high flux magnetic extracting and enriching, comprise the steps:
(1) pour the pending sample solution containing the extraction particle that is magnetic into some sampling receptacle, be positioned over the container deposit position of container tray;
(2) regulate enrichment probe bracket that enrichment is popped one's head in and drop to enrichment probe lower end insertion sample solution or regulate container tray to rise to sample solution dipped enrichment probe lower end;
(3) enrichment probe magnetic is regulated to make enrichment probe lower end have magnetic or magnetic enhancement;
(4) a period of time is kept to make enrichment probe lower end fully adsorb magnetic extraction particle;
(5) regulate enrichment probe bracket that enrichment is popped one's head in and rise or regulate container tray decline to make enrichment pop one's head in lower end higher than edge on container;
(6) container tray being loaded with sampling receptacle is removed, replace with the container tray that placed the container filling elute soln;
(7) regulate enrichment probe bracket that enrichment is popped one's head in and drop to enrichment probe lower end insertion elute soln or regulate container tray to rise to elute soln dipped enrichment probe lower end;
(8) regulate enrichment probe magnetic that enrichment probe lower end magnetic is disappeared or weaken to not adsorbing magnetic extraction particle;
(9) a period of time is kept to make enrichment pop one's head in lower end absorption magnetic extraction particle fully by wash-out;
(10) regulate enrichment probe bracket that enrichment is popped one's head in and rise or regulate container tray decline to make enrichment pop one's head in lower end higher than edge on container;
(11) container tray being loaded with elution container is removed, collect the elute soln containing the extraction particle that is magnetic.
Further, in step (4) and (9), the regulating device of adjustment to-and-fro movement, circular motion makes enrichment probe stir solution.
Enrichment probe with annular plate washer needs to regulate enrichment probe to immerse the degree of depth of solution to ensure that the magnetic extraction particle of below ring baffle can by abundant wash-out.
Above-mentioned steps carries out Comprehensive Control by presetting apparatus to each regulating device, to regulate the amplitude, speed, duration, order, interval time etc. of each type games, thus realizes automation function.
Repeatedly repeat above-mentioned steps, the high flux process of batch samples solution can be achieved.
The kind of magnetic extraction particle and elute soln is selected according to the character of pending sample, adopts prior art to realize, does not belong to content of the present invention.
The above is only the preferred embodiment of the present invention, therefore, for those skilled in the art, under the prerequisite not departing from this principle of device, if make relevant retouching or improve, also should be considered as protection scope of the present invention.
Claims (10)
1. a high flux magnetic extracting and enriching device, comprises the adjustable enrichment probe of magnetic, enrichment probe bracket, support bar, container tray, base; It is characterized in that some enrichments probe is fixed on enrichment probe bracket, enrichment probe bracket is connected with support bar, and support bar is connected with base, and container tray is positioned at enrichment probe below, and container tray arranges the container deposit position of popping one's head in corresponding with enrichment; Container tray is connected with support bar or base, or container tray is positioned on base or tray supporter, and tray supporter is connected with support bar or base; Enrichment probe bracket, tray supporter, container tray, support bar and/or base are provided with one or more regulating device making enrichment probe and container tray do relative motion.
2. enriching apparatus according to claim 1, is characterized in that the adjustable enrichment probe of magnetic is adopt electromagnetism regulate the enrichment probe of magnetic or adopt the enrichment probe of permanent magnet mechanical adjustment magnetic.
3. enriching apparatus according to claim 1, it is characterized in that adopting electromagnetism regulate the enrichment of magnetic probe comprise electromagnet, electromagnet fixed bar, for regulating magnet magnetic strong and weak or with presence or absence of electromagnetic control device; Electromagnet fixed bar one end fixed electromagnet, the other end is fixed on enrichment probe bracket.
4. enriching apparatus according to claim 1, it is characterized in that adopting the enrichment of permanent magnet mechanical adjustment magnetic to pop one's head in the permanent magnet, the outer tube that comprise interior rod, be connected with interior excellent lower end, outer sleeve upper open lower end is cecum, and permanent magnet can move up and down in outer tube with interior rod; Enrichment probe bracket is divided into two layers, upper layer bracket is connected with interior excellent top, lower floor support is connected with outer tube top, and upper and lower layer support is connected with support bar respectively, and support and/or support bar are provided with one or more regulating device making levels support do relative motion.
5., according to enriching apparatus according to claim 1, it is characterized in that container deposit position is container deposit groove or container deposit hole; Container tray and tray supporter, support bar or base connected mode are that buckle-type is connected.
6. enriching apparatus according to claim 1, is characterized in that the regulating device making enrichment probe and container tray do relative motion comprises:
The regulating device that enrichment probe bracket is moved up and down that enrichment probe bracket or support bar are arranged; And/or, the regulating device that container tray is moved up and down that container tray or support bar or base are arranged;
The enrichment probe bracket that makes that enrichment probe bracket or support bar are arranged makes the regulating device of to-and-fro movement by a small margin, circular motion or significantly translation motion; And/or the container tray that makes arranged on container tray or support bar or base makes the regulating device of to-and-fro movement by a small margin, circular motion or significantly translation motion.
7. enriching apparatus according to claim 4, is characterized in that the regulating device making levels support do relative motion comprises:
Upper layer bracket or support bar arrange the regulating device that upper layer bracket is moved up and down; And/or, lower floor support or support bar arrange the regulating device that lower floor support is moved up and down;
Upper layer bracket or support bar are arranged and makes the to-and-fro movement by a small margin of upper layer bracket do, the regulating device of circular motion; And/or, lower floor support or support bar are arranged and makes the to-and-fro movement by a small margin of lower floor support do, the regulating device of circular motion.
8. the enriching apparatus according to claim 6 or 7, it is characterized in that moving up and down regulating device be swing pinion shoulder with band tooth track combination, screw and rotating wire combine, position limiting slide block and track combination or pilot pin and pilot hole combine; The regulating device of to-and-fro movement, circular motion is push cam mechanism, toggle, back and forth screw mechanism or fan-shaped rotary reciprocator; Significantly the regulating device of translation motion is guide rail, turning axle or curved bar.
9. enriching apparatus according to claim 4, is characterized in that the middle and lower part outside surface of outer tube is provided with ring baffle.
10. adopt device described in claim 1 to carry out a method for high flux magnetic extracting and enriching, comprise the steps:
(1) pour the pending sample solution containing the extraction particle that is magnetic into some sampling receptacle, be positioned over the container deposit position of container tray;
(2) regulate enrichment probe bracket that enrichment is popped one's head in and drop to enrichment probe lower end insertion sample solution or regulate container tray to rise to sample solution dipped enrichment probe lower end;
(3) enrichment probe magnetic is regulated to make enrichment probe lower end have magnetic or magnetic enhancement;
(4) a period of time is kept to make enrichment probe lower end fully adsorb magnetic extraction particle;
(5) regulate enrichment probe bracket that enrichment is popped one's head in and rise or regulate container tray decline to make enrichment pop one's head in lower end higher than edge on container;
(6) container tray being loaded with sampling receptacle is removed, replace with the container tray that placed the container filling elute soln;
(7) regulate enrichment probe bracket that enrichment is popped one's head in and drop to enrichment probe lower end insertion elute soln or regulate container tray to rise to elute soln dipped enrichment probe lower end;
(8) regulate enrichment probe magnetic that enrichment probe lower end magnetic is disappeared or weaken to not adsorbing magnetic extraction particle;
(9) a period of time is kept to make enrichment pop one's head in lower end absorption magnetic extraction particle fully by wash-out;
(10) regulate enrichment probe bracket that enrichment is popped one's head in and rise or regulate container tray decline to make enrichment pop one's head in lower end higher than edge on container;
(11) container tray being loaded with elution container is removed, collect the elute soln containing the extraction particle that is magnetic;
Further, in step (4) and (9), the regulating device of adjustment to-and-fro movement, circular motion makes enrichment probe stir solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915818.0A CN105527150B (en) | 2015-12-10 | 2015-12-10 | A kind of high-throughput magnetic extracting and enriching device and enrichment method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510915818.0A CN105527150B (en) | 2015-12-10 | 2015-12-10 | A kind of high-throughput magnetic extracting and enriching device and enrichment method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105527150A true CN105527150A (en) | 2016-04-27 |
| CN105527150B CN105527150B (en) | 2018-10-23 |
Family
ID=55769513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510915818.0A Expired - Fee Related CN105527150B (en) | 2015-12-10 | 2015-12-10 | A kind of high-throughput magnetic extracting and enriching device and enrichment method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105527150B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107456777A (en) * | 2017-08-07 | 2017-12-12 | 胡国良 | Catch tray |
| CN109175181A (en) * | 2018-07-11 | 2019-01-11 | 北京科技大学 | High throughput forging rotating platform and method |
| CN110327659A (en) * | 2019-07-19 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of extraction and purification method and device based on magnetic fiber material |
| CN111157325A (en) * | 2020-01-07 | 2020-05-15 | 沈丹 | Organic matter extraction device for ecological environment detection |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6447729B1 (en) * | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
| US6448092B1 (en) * | 1994-10-20 | 2002-09-10 | Thermo Labsystems Oy | Separation device for microparticles involving a magnetic rod |
| WO2006081995A1 (en) * | 2005-02-02 | 2006-08-10 | Chemagen Biopolymer-Technologie Ag | Device and method for the elimination of magnetic or magnetizable particles from a liquid |
| CN102656465A (en) * | 2009-12-16 | 2012-09-05 | 株式会社日立高新技术 | Sample processing device, sample processing method, and reaction container used in these device and method |
| CN103391815A (en) * | 2011-02-23 | 2013-11-13 | 恩姆菲舍尔科技公司 | Particle processing |
| CN103805508A (en) * | 2013-07-16 | 2014-05-21 | 北京金麦格生物技术有限公司 | Instrument and method for extracting bioactive substances with magnetic particle method |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN103923831A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Instrument and method for extraction of bioactive substances |
| CN104344985A (en) * | 2013-08-09 | 2015-02-11 | 英芮诚生化科技（上海）有限公司 | Magnetic separation device capable of sequentially extracting and continuously eluting trace substances |
| CN205209857U (en) * | 2015-12-10 | 2016-05-04 | 南京医科大学 | High flux magnetism extraction enrichment device |
-
2015
- 2015-12-10 CN CN201510915818.0A patent/CN105527150B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6447729B1 (en) * | 1993-02-01 | 2002-09-10 | Labsystems Oy | Method and means for magnetic particle specific binding assay |
| US6448092B1 (en) * | 1994-10-20 | 2002-09-10 | Thermo Labsystems Oy | Separation device for microparticles involving a magnetic rod |
| WO2006081995A1 (en) * | 2005-02-02 | 2006-08-10 | Chemagen Biopolymer-Technologie Ag | Device and method for the elimination of magnetic or magnetizable particles from a liquid |
| CN102656465A (en) * | 2009-12-16 | 2012-09-05 | 株式会社日立高新技术 | Sample processing device, sample processing method, and reaction container used in these device and method |
| CN103391815A (en) * | 2011-02-23 | 2013-11-13 | 恩姆菲舍尔科技公司 | Particle processing |
| CN103923831A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Instrument and method for extraction of bioactive substances |
| CN103805508A (en) * | 2013-07-16 | 2014-05-21 | 北京金麦格生物技术有限公司 | Instrument and method for extracting bioactive substances with magnetic particle method |
| CN104344985A (en) * | 2013-08-09 | 2015-02-11 | 英芮诚生化科技（上海）有限公司 | Magnetic separation device capable of sequentially extracting and continuously eluting trace substances |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN205209857U (en) * | 2015-12-10 | 2016-05-04 | 南京医科大学 | High flux magnetism extraction enrichment device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107456777A (en) * | 2017-08-07 | 2017-12-12 | 胡国良 | Catch tray |
| CN107456777B (en) * | 2017-08-07 | 2020-01-14 | 胡国良 | Collecting tray |
| CN109175181A (en) * | 2018-07-11 | 2019-01-11 | 北京科技大学 | High throughput forging rotating platform and method |
| CN109175181B (en) * | 2018-07-11 | 2020-02-28 | 北京科技大学 | High-flux forging rotary platform and method |
| CN110327659A (en) * | 2019-07-19 | 2019-10-15 | 天津迪沃特生物电子科技有限公司 | A kind of extraction and purification method and device based on magnetic fiber material |
| CN111157325A (en) * | 2020-01-07 | 2020-05-15 | 沈丹 | Organic matter extraction device for ecological environment detection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105527150B (en) | 2018-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105527150A (en) | High-throughput magnetic extraction enrichment device and enrichment method | |
| CN204447405U (en) | A kind of discoid rotatable solid-phase extraction device | |
| US7384559B2 (en) | Device and method for treating magnetic particles | |
| JP4792192B2 (en) | System and method for manipulating magnetically responsive particles in a liquid sample and collecting DNA or RNA from the sample | |
| JP4975645B2 (en) | Apparatus and method for removing magnetic or magnetizable particles from a liquid | |
| US8371743B2 (en) | Method for suspending or re-suspending particles in a solution and apparatus adapted thereto | |
| CN110327659B (en) | Extraction and purification method and device based on magnetic extraction material | |
| JP2009513318A (en) | Apparatus and method for separating magnetic or magnetizable particles from a liquid | |
| CN205209857U (en) | High flux magnetism extraction enrichment device | |
| CN205192832U (en) | Enrichment probe with adjustable magnetism | |
| CN110658351A (en) | Automatic sampler for magnetic separation of magnetic iron in iron ore in laboratory and use method | |
| CN104117429B (en) | A kind of heating oscillating magnetic flux separator | |
| CN205528702U (en) | Magnetic beads sorting device | |
| CN208260249U (en) | Magnetic solid phase extraction washing device | |
| Burguera et al. | On-line electrothermal atomic absorption spectrometry configurations: recent developments and trends | |
| US20010024624A1 (en) | Vial handling system with improved mixing mechanism | |
| CN206567091U (en) | A kind of magnetic purifies and separates device | |
| CN105659096A (en) | Sample preparation workstation | |
| CN104215497A (en) | Experimental device and method for extracting polycyclic aromatic hydrocarbons in enriched seawater | |
| CN210347273U (en) | Online automatic magnetic nano-material oscillation-desorption device | |
| CN105598110A (en) | Automatic cleaning method with solid-liquid separation | |
| CN112229691B (en) | Device and method for acidolysis by using I-shaped glass tube | |
| CN114858567A (en) | Efficient magnetic solid-phase extraction device and extraction method based on in-situ magnetic control | |
| CN210719896U (en) | Full-automatic solid phase extraction appearance | |
| CN208346194U (en) | A kind of Rapid nucleic acid extract equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181023 Termination date: 20191210 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |