CN103923831A - Instrument and method for extraction of bioactive substances - Google Patents
Instrument and method for extraction of bioactive substances Download PDFInfo
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- CN103923831A CN103923831A CN201310014908.3A CN201310014908A CN103923831A CN 103923831 A CN103923831 A CN 103923831A CN 201310014908 A CN201310014908 A CN 201310014908A CN 103923831 A CN103923831 A CN 103923831A
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- needle
- sleeve pipe
- end block
- lever arm
- instrument
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention provides an efficient automated instrument for extraction of bioactive substances. The instrument comprises: a housing, a base plate, a workbench, magnetic needles and a magnetic needle moving mechanism, casing pipes and a casing pipe moving mechanism, and a vibrating mechanism. The vibrating mechanism can generate vibration to make the casing pipe moving mechanism vibrate up and down. Specifically, the magnetic needles can be vertically inserted into and removed from the casing pipes, and the casing pipes can be vertically inserted into and removed from a sample treatment area. The vibrating mechanism comprises a lever arm, a movable fulcrum and a rotating mechanism. According to the arrangement, no matter how the vibration amplitude changes, the lowest point of the amplitude does not change. The invention also provides a method using the instrument to extract and purify bioactive substances, particularly nucleic acid. The instrument provided by the invention can be arbitrarily adjust the vibration frequency and amplitude, greatly improve the adjustment range of vibration frequency, and has the advantages of low noise and long life.
Description
Technical field
The present invention relates to biology and apparatus field.Concrete, the invention provides a kind of self-reacting device that extracts biologically active substance, and use described instrument to extract the method for biologically active substance.
Background technology
Extract biologically active substance at multiple fields such as modern clinic medical diagnosis on disease, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology researches, for example cell, and the biologically active substance such as protein, nucleic acid has vital role.Wherein, the biologically active substance compound that nucleic acid is aggregated into by much Nucleotide, is one of base substance of life.Nucleic acid is extensively present in all animals, vegetable cell, microorganism, in organism.According to chemical constitution difference, nucleic acid can be divided into Yeast Nucleic Acid, is called for short RNA and thymus nucleic acid, is called for short DNA.DNA is the essential substance basis that stores, copies and convey hereditary information, and RNA plays an important role in protein building-up process.The development of biotechnology is maked rapid progress, along with round pcr is in the application of every field, comprise that medical science aspect disease detection, agriculture aspect transgenosis detect and other very many-sided application etc., need high-throughout sample biologically active substance to extract and method and instrument that full-automatic biologically active substance extracts.
Utilize paramagnetic particle method extract biologically active substance particularly active macromole be the technology that developed recently gets up.Paramagnetic particle method be utilize can specific recognition cell, the magnetic bead of protein or nucleic acid, this magnetic bead can be combined specifically with nucleic acid molecule, then under the effect in magnetic field, the active macromole from blood, animal tissues, food, pathogenic micro-organism equal samples is separated.For example, the paramagnetic particle method of nucleic acid is extracted can be according to the principle identical with pellosil centrifugal column, uses nanotechnology to modify the surface of superparamagnetic nano particle, is prepared into superparamagnetism monox nanometer magnetic bead.This magnetic bead can be identified specifically with nucleic acid molecule.Utilize the superparamagnetism of monox nanometer microballoon, under the effect of Chaotropic salt (Guanidinium hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field, can from sample, DNA and RNA be separated.
Paramagnetic particle method automatic extracting instrument is divided into again bar magnet method and suction method.Suction method is to be undertaken by automatic pipetting device.Taking paramagnetic particle method instrument for extracting nucleic acid as example, can add lysate, magnetic bead absorption by automatic pipetting device; Take solution away, add rinsing liquid, magnetic bead absorption, takes rinsing liquid away, adds elution buffer.When there is removal waste liquid due to suction method, do not take magnetic bead away, pipettor can not lean on magnetic bead too near, in case the problem that magnetic bead is taken out together with waste liquid affects elution efficiency and purity.
It is the technology that developed recently gets up that bar magnet method is extracted, and its principle is that magnetic separating device is immersed to liquid, makes can be concentrated on separating device surface by the particle of magnetic attraction in mixture by the action of a magnetic field.Wherein, in a kind of mode, magnetic separating device is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attract can be adsorbed onto sleeve surface by the particle of magnetic attraction in mixture, in the time that bar magnet is detached, sleeve pipe loses magnetism, and is departed from sleeve pipe by the particle of magnetic attraction.The flux of bar magnet method instrument for extracting nucleic acid has 8,16,32,96 passages conventionally, and with respect to suction method, the advantage of bar magnet method is that the liquid of each step is not residual, because bar magnet is only taken away magnetic bead, transfers in the corresponding reacting hole of next step.In bar magnet method instrument, drive the mechanism of bar magnet can drive bar magnet to move up and down in sleeve pipe, contribute to cracking and rinsing and the purifying of leaching process.
Summary of the invention
The invention provides a kind of self-reacting device that utilizes efficiently bar magnet method to extract biologically active substance, can be applicable to the multiple fields such as clinical disease diagnosis, transfusion safety, Forensic Identification, environmental microorganism detection, food safety detection, molecular biology research, for example, for extracting and purifying biological active substance, cell, albumen and nucleic acid.
Concrete, the invention provides a kind ofly for extracting the instrument of biologically active substance, comprising:
Shell and base plate 1,
Worktable 15, has sample preparation district on it,
Needle 7 and needle travel mechanism 48, needle travel mechanism comprises needle support 8 and needle lifting mechanism 33, needle is removably fixed on needle support, wherein, by moving up and down of needle lifting mechanism, makes needle can vertically insert and depart from sleeve pipe,
Sleeve pipe 4 and sleeve pipe travel mechanism 16, sleeve pipe travel mechanism comprises casing support 6 and telescopic lifter structure 18, sleeve pipe is removably fixed on casing support, wherein, by moving up and down of telescopic lifter structure, make sleeve pipe vertically to insert and to depart from sample preparation district, in addition, sleeve pipe travel mechanism 16 can up-down vibration;
For supporting the mechanism rack 13 of described needle travel mechanism 48 and sleeve pipe travel mechanism 16,
With
Vibrating mechanism 19, described vibrating mechanism can produce and export vibration, makes sleeve pipe travel mechanism 16 carry out up-down vibration, wherein,
Vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, and wherein said rotating mechanism has wheel disc and axle center, and when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produces vibration.An aspect therein, instrument of the present invention is characterised in that, described lever arm, anyway the amplitude size of vibration producing that is arranged so that of movable fulcrum and rotating mechanism changes, and its lower-most point of amplitude of exporting to the vibration of sleeve pipe travel mechanism is constant.Thus, no matter vibrating mechanism produces and how the amplitude of the vibration of output changes, and also no matter how its conduction changes to the amplitude of casing support, and the lower-most point of the amplitude of the pipe box of instrument of the present invention is constant.
Rotating mechanism in the vibrating mechanism of instrument of the present invention has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotate around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and output vibration.Of the present invention, aspect one of them, described rotating mechanism is eccentric rotary mechanism, and the Bu center, axle center of its rotatable parts, is eccentric rotary mechanism.Eccentric rotary mechanism can be eccentric wheel etc.
In the present invention, the point that is formed into the shortest distance in its axle center in the profile outer rim of described rotating mechanism (particularly eccentric rotary mechanism) is called nearest profile point.In the present invention, the working order of vibrating mechanism refers to that the rotating mechanism of vibrating mechanism rotates around axle center, the state when outer rim promotion lever arm of its wheel disc rotates around balance pivot.In the present invention, in the time that the nearest profile point of rotating mechanism described in vibrating mechanism produces pushing effect (the nearest profile point that is generally rotating mechanism contacts with lever arm) to lever arm, in vibrating mechanism, lever arm rotates around balance pivot, now can be called lowest amplitude position of the fulcrum around the position of rotating lever fulcrum for lever arm.In one embodiment of the present invention, in instrument of the present invention, the lever arm of vibrating mechanism, being set to of movable fulcrum and rotating mechanism, in working order under, the variation of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum.Thus, how the displacement that can make the rotating mechanism of vibrating mechanism no matter promote lever arm changes, also no matter how the position of movable fulcrum on lever arm is adjusted, also no matter vibrating mechanism produces and how the amplitude of the vibration of output changes, the lower-most point of the amplitude of the vibration of its output is all constant, thereby how the amplitude that makes vibrating mechanism no matter export to casing support changes, and the lower-most point of its amplitude is constant, also make the lower-most point of amplitude of the pipe box of instrument of the present invention constant.In instrument of the present invention, vibrating mechanism can be by a fixing some output amplitude on lever arm, and this point is normally relatively rotating the opposite side of fulcrum of mechanism and lever arm point of contact.How the amplitude no matter vibrating mechanism is exported to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, also makes the lower-most point of amplitude of the pipe box of instrument of the present invention constant.
The rotating mechanism using in the present invention can be eccentric rotary mechanism, and it has the wheel disc of axle center (being turning point) and ad hoc type line profile, Bu center, its axle center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.Adopt circular wheel to have easily manufactured, technique is simple, and the amplitude of output vibration is easy to the advantages such as adjusting.In addition, the cam that has a non-central axle center also can be used as eccentric rotary of the present invention mechanism.
Have central hub but can also can be used for the present invention by vibrative rotating mechanism, for example outline is oval cam.
In the present invention, movable fulcrum can be that fulcrum contacts or embed with the lower edge of lever arm the groove or the hollow groove etc. that are arranged on lever arm with the way of contact of lever arm.
In one embodiment of the invention, movable fulcrum is multiple can independently separation with lever arm or the fulcrum of contact, comes in contact and forms single fulcrum respectively as required with lever arm, can produce thus the vibration with particular amplitude.For example, can be below lever arm or side multiple fulcrum piece or fulcrum shafts that can lifting or move horizontally be set, each fulcrum piece or fulcrum shaft can contact with lever arm lower edge in lifting, or mobile lever arm lower edge or the groove of embedding, respectively the independent fulcrum that forms.
In another embodiment of the present invention, movable fulcrum is set to or to be arranged on groove on lever arm or mobile fulcrum that hollow groove moves, the vibration that can produce thus the amplitude with continuous variable along the lower edge of lever arm.For example, movable fulcrum is mounted in the fulcrum shaft on Mobile base, and fulcrum shaft contacts with the lower edge of lever arm, or inserts groove or hollow groove on lever arm, under the driving of Mobile base, moves formation fulcrum along lever arm.
Lever arm is preferably set to linear lever arm.Described lever arm can have the groove parallel with its upper surface or hollow groove, can be for contacting for fulcrum.Mobile fulcrum can move along the edge of lever arm (lower edge) or groove or the hollow groove be located on lever arm, and its travel direction is the axial direction due of lever arm.In this case, the axle center that vibrating mechanism is set to eccentric rotary mechanism equates to the distance of lever arm with described axle center to the minor increment of profile outer rim.In the time that eccentric rotary mechanism is eccentric wheel, vibrating mechanism is set to eccentric minimum radius (being the minor increment that wheel rim is arrived in axle center) and equates to the distance of lever arm with its axle center.In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (being the axial of lever arm) that lever moves fulcrum.
Lever arm also can be the lever arm of broken-line type or shaped form.
Of the present invention wherein aspect another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, under traversing carriage driving mechanism drives, in hollow groove, move, form the mobile fulcrum of lever.
In one aspect of the invention, lever arm has vibrating wheels 25, and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
In one aspect of the invention, in the instrument of the invention described above, needle support 8 comprises needle support end block 9 and needle supported arm 34, and needle is removably fixed on needle supported arm; Described needle lifting mechanism comprises that the needle that is arranged on needle support end block below promotes end block 17, when needle promotion end block moves upward, promotes needle support end block and moves upward, and moves upward thereby promote needle support.Of the present invention aspect another, in above-mentioned instrument, needle promotion end block 17 is separable with needle support 8, when needle support contacts with casing support in the process declining, needle promotes end block and separates with needle support, and needle support is supported by casing support and together moves with casing support.
In one aspect of the invention, in the instrument of the invention described above, casing support 6 comprises casing support end block 35 and casing support arm 5, and sleeve pipe is removably fixed on casing support arm; Described telescopic lifter structure comprises the sleeve pipe lifting end block 36 that is arranged on casing support end block below, when sleeve pipe lifting end block moves upward, pushing set tube support end block moves upward, thereby pushing set tube support moves upward, when lifting end block moves downward, casing support end block is synchronizeed and is moved downward with sleeve pipe lifting end block according to action of gravity.Of the present invention, aspect another, described in above-mentioned instrument, sleeve pipe lifting end block and casing support are separable.In addition, in one embodiment of the invention, the casing support end block below of above-mentioned instrument arranges cover tube vibration push rod 26, and it is connected with vibrating mechanism 19, conduction vibration.
In one aspect of the invention, in the instrument of the invention described above, casing support has at least one casing support arm, preferred, and casing support has two casing support arms, on supported arm, have can fixed sleeving bushing slot.
In one aspect of the invention, in the instrument of the invention described above, needle support has at least one needle supported arm, and preferred, needle support has two supported arms, has the needle groove that can fix needle on supported arm.
In one aspect of the invention, in the instrument of the invention described above, worktable can move horizontally.Wherein sample preparation district can comprise workspace, sample cell or sample well, or its combination.Of the present invention, aspect another, described sample preparation district has bottoming hole, its below configuration well heater.
In one aspect of the invention, needle described in the instrument of the invention described above is permanent magnet material.
In one aspect of the invention, sleeve pipe described in the instrument of the invention described above is nonmagnetic substance.
The instrument of the invention described above can for example, for extracting biologically active substance, cell, protein or nucleic acid.
The instrument of the invention described above can be for for extracting and the instrument of purification of nucleic acid.
The present invention also provides a kind of method that uses above-mentioned instrument extraction and purifying biological active substance.
In another aspect of the present invention, provide a kind of for adopting the vibrating mechanism of biologically active substance extraction apparatus of bar magnet method, described extraction apparatus has needle and needle travel mechanism, and sleeve pipe and sleeve pipe travel mechanism, by moving up and down of needle travel mechanism, make needle can vertically insert and depart from sleeve pipe, by moving up and down of sleeve pipe travel mechanism, make sleeve pipe can vertically insert and depart from the sample preparation district of its below, in addition, described vibrating mechanism can produce vibration, drive the sleeve pipe up-down vibration of described extraction apparatus, it is characterized in that, described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produce vibration.In the present invention, the lever arm of above-mentioned vibrating mechanism, anyway the amplitude size of vibration producing that is arranged so that of movable fulcrum and rotating mechanism changes, and its lower-most point of amplitude of exporting to the vibration of sleeve pipe travel mechanism is constant.
Rotating mechanism in vibrating mechanism of the present invention has wheel disc and the axle center of ad hoc type line profile, and when these rotatable parts rotate around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, produces and output vibration.Of the present invention, aspect one of them, described rotating mechanism is eccentric rotary mechanism, and the Bu center, axle center of its rotatable parts, is eccentric rotary mechanism.Eccentric rotary mechanism can be eccentric wheel etc.
An aspect therein, the lever arm of vibrating mechanism of the present invention, being set to of movable fulcrum and rotating mechanism, in working order under, the variation of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum.Thus, how the displacement that can make the rotating mechanism of vibrating mechanism no matter promote lever arm changes, also no matter how the position of movable fulcrum on lever arm is adjusted, also no matter vibrating mechanism produces and how the amplitude of the vibration of output changes, described in it, the lower-most point of amplitude is all constant, thereby how the amplitude that makes vibrating mechanism no matter export to the casing support of biologically active substance extraction apparatus changes, the lower-most point of its amplitude is constant, also makes the lower-most point of amplitude of the pipe box of instrument of the present invention constant.Conventionally, vibrating mechanism is by a fixing some output amplitude on lever arm, and this point is normally relatively rotating the opposite side of fulcrum of mechanism and lever arm point of contact.How the amplitude no matter vibrating mechanism is exported to casing support changes, constant in the lower-most point of the amplitude of this point of fixity, also makes the lower-most point of amplitude of the pipe box of instrument of the present invention constant.
The rotating mechanism using in vibrating mechanism of the present invention can be eccentric rotary mechanism, and it has the wheel disc of axle center (being turning point) and ad hoc type line profile, Bu center, its axle center.Preferably, described rotating mechanism can adopt circular as profile, is eccentric wheel.Adopt circular wheel to have easily manufactured, technique is simple, and the amplitude of output vibration is easy to the advantages such as adjusting.In addition, the cam that has a non-central axle center also can be used as eccentric rotary of the present invention mechanism.
Have central hub but can also can be used for vibrating mechanism of the present invention by vibrative rotating mechanism, for example outline is oval cam.
In vibrating mechanism of the present invention, movable fulcrum can be that fulcrum contacts or embed with the lower edge of lever arm the groove or the hollow groove etc. that are arranged on lever arm with the way of contact of lever arm.
In a kind of embodiment of vibrating mechanism of the present invention, movable fulcrum is multiple can independently separation with lever arm or the fulcrum of contact, comes in contact and forms single fulcrum respectively as required with lever arm, can produce thus the vibration with particular amplitude.For example, can be below lever arm or side multiple fulcrum piece or fulcrum shafts that can lifting or move horizontally be set, each fulcrum piece or fulcrum shaft can contact with lever arm lower edge in lifting, or mobile lever arm lower edge or the groove of embedding, respectively the independent fulcrum that forms.
In another embodiment of vibrating mechanism of the present invention, movable fulcrum is set to or to be arranged on groove on lever arm or mobile fulcrum that hollow groove moves, the vibration that can produce thus the amplitude with continuous variable along the lower edge of lever arm.For example, movable fulcrum is mounted in the fulcrum shaft on Mobile base, and fulcrum shaft contacts with the lower edge of lever arm, or inserts groove or hollow groove on lever arm, under the driving of Mobile base, moves formation fulcrum along lever arm.
In vibrating mechanism of the present invention, lever arm is preferably set to linear lever arm.Described lever arm can have the groove parallel with its upper surface or hollow groove, can be for contacting for fulcrum.Mobile fulcrum can move along the edge of lever arm (lower edge) or groove or the hollow groove be located on lever arm, and its travel direction is the axial direction due of lever arm.In this case, the axle center that vibrating mechanism is set to eccentric rotary mechanism equates to the distance of lever arm with described axle center to the minor increment of profile outer rim.In the time that eccentric rotary mechanism is eccentric wheel, vibrating mechanism is set to eccentric minimum radius (being the minor increment that wheel rim is arrived in axle center) and equates to the distance of lever arm with its axle center.In this case, the radial direction of eccentric wheel minimum radius is vertical with the travel direction (being the axial of lever arm) that lever moves fulcrum.
In vibrating mechanism of the present invention, lever arm also can be the lever arm of broken-line type or shaped form.
In vibrating mechanism of the present invention aspect another, mobile fulcrum comprises traversing carriage 23, traversing carriage driving mechanism 24 and mobile fulcrum shaft 37, lever arm has the hollow groove parallel with its upper surface, mobile fulcrum shaft is through hollow groove, under traversing carriage driving mechanism drives, in hollow groove, move, form the mobile fulcrum of lever.
Aspect of vibrating mechanism of the present invention, lever arm has vibrating wheels 25, and described vibrating wheels contacts with telescopic lifter structure, conduction vibration, and described vibrating wheels and rotating mechanism lay respectively at the both sides of mobile fulcrum.
The present invention also provide a kind of vibrating mechanism that uses the invention described above in bar magnet method biologically active substance extraction apparatus to the vibrative method of the sleeve pipe of extraction apparatus.
Brief description of the drawings
By reading the detailed description that non-limiting example is done of doing with reference to the following drawings, it is more obvious that other features, objects and advantages of the present invention will become:
Fig. 1 is first direction stereographic map in accordance with a preferred embodiment of the present invention;
Fig. 2 is second direction stereographic map in accordance with a preferred embodiment of the present invention;
Fig. 3 is side cross-sectional schematic in accordance with a preferred embodiment of the present invention;
Fig. 4 is side cross-sectional schematic in accordance with a preferred embodiment of the present invention;
Fig. 5 is sectional block diagram in accordance with a preferred embodiment of the present invention.
Reference numeral
1 base plate
2 heated sample grooves
3 non-heating work districts
4 sleeve pipes
5 casing support arms
6 casing supports
7 needles
8 needle supports
9 needle support end blocks
10 optical axises
11 motors one
12 motors two
13 mechanism racks
14 mechanism's platforms
15 traversers
16 sleeve pipe travel mechanisms
17 needles promote end block
18 telescopic lifter structures
19 vibrating mechanisms
20 eccentric wheel drive-motor
21 eccentric wheels
22 lever arms
23 traversing carriages
24 traversing carriage driving mechanisms
25 vibrating wheels
26 cover tube vibration push rods
31 screw mandrels one
32 screw mandrels two
33 needle lifting mechanisms
34 needle supported arms
35 casing support end blocks
36 sleeve pipe lifting end blocks
37 move fulcrum shaft
48 needle travel mechanisms
Embodiment
For a better understanding and interpretation of the present invention, the present invention is described in further detail below with reference to accompanying drawings.
Fig. 1 be according to a preferred embodiment of the present invention for extracting the first direction stereographic map of inside of instrument of biologically active substance.Instrument of the present invention can be used for extracting and purification of nucleic acid.This instrument has shell (not illustrating at accompanying drawing) and base plate 1, needle 7 and needle travel mechanism 48, sleeve pipe 4 and sleeve pipe travel mechanism 16, for supporting the support 13 of described needle travel mechanism and sleeve pipe travel mechanism, and for generation of vibration, make sleeve pipe travel mechanism carry out the vibrating mechanism 19 of up-down vibration.
Shell (not illustrating at accompanying drawing) can be by spaced apart to instrument internal and atmosphere, in order to avoid sample contamination and damage parts.Housing face-plate is generally metallic substance and makes.Shell can have the sample window that can open and close, for putting into and take out sample.Shell can have the viewing window made from transparent material.Sample window and viewing window can be same parts.Shell can have the external apparatus interface being connected with peripheral equipment in addition, the display screen of operation display interface, various manipulation of switches, button or button etc.
Sleeve pipe is the pipe with closed bottom and hollow tube chamber.Sleeve pipe can have for example elongated cylinder form.The material of sleeve pipe is normally nonmagnetic.The Choice and design of its material and thickness is for weakened field not or only affect a little magnetic field, but need to ensure can attract in the outer generation of sleeve pipe the magnetic field of magnetic bead or the biological sample with magnetic bead in the time inserting needle.
Needle can vertically insert and depart from sleeve pipe.The bottom (inserting one end of sleeve pipe) of needle has magnetic.When needle inserts when contact or approach sleeve bottom, can produce magnetic field outward at sleeve pipe, thereby realize sleeve pipe to sleeve pipe absorption and the disengaging of the magnetic bead in solution or the biological sample with magnetic bead outward.In the present embodiment, the bottom of needle has permanent magnet, and band is magnetic thus.In other embodiment of the present invention, needle can produce magnetic (electromagnet) by electromagnetic action.
Fig. 2 be above-mentioned according to a preferred embodiment of the invention for extracting the second direction stereographic map of instrument of biologically active substance.
As depicted in figs. 1 and 2, described have the worktable 15 that can move horizontally for extracting the instrument of biologically active substance.On the worktable 15 that can move horizontally as shown in Figure 2,, there is sample preparation district.Sample preparation district can comprise workspace, sample cell, sample well, or its combination.Sample preparation district for arrange can processing sample sample cell or sample well, or for placing the vessel of load sample, as type culture plates such as single or single centrifuge tube, 24 orifice plates or 96 orifice plates, also can put the non-standard culture plate needing.
In illustrated the preferred embodiments of the present invention instrument, worktable has Liang Zu sample preparation district with corresponding two groups of needles and cannula system.Mei Zu sample preparation district comprises respectively the non-heating work district 3 in two groups of heated sample grooves 2 and these two groups of sample cells.Heated sample groove can insert the containers such as single or single centrifuge tube.The containers such as single or single centrifuge tube can be put by non-heating work district, also can put the type culture plate such as 24 orifice plates or 96 orifice plates, can also put the non-standard culture plate needing.Worktable 15 is by the driving mechanisms control that is arranged at its below, can move in the horizontal direction, make sample preparation district move to the below of sleeve pipe, sleeve pipe can vertically insert and depart from the sample cell in sample preparation district, or the test tube or the EP that are fixed on sample cell manage, or the hole of culture plate.
Described have needle 7 and needle travel mechanism 48 for extracting the instrument of biologically active substance.Needle travel mechanism 48 comprises needle support 8 and needle lifting mechanism 33.
Needle support 8 comprises needle support end block 9 and needle supported arm 34.Needle is removably fixed on needle supported arm.Diagram the preferred embodiments of the present invention for extracting the instrument of biologically active substance, needle support has two needle supported arms that be arranged in parallel.Needle support also can only have a needle supported arm, can be also to exceed two.The quantity of the fixable needle of each needle supported arm can be adjusted as required, and common every group is 1-12 root needle, and common is 4,6 or 12 needles.At another embodiment, needle supported arm can be fixed needle group, and each needle group has many row's needles, 24 needles (spread pattern is 4 × 6) of for example corresponding culture plate, 96 needles (spread pattern is 8 × 12) etc.In the present embodiment, the bottom of needle has permanent magnet, and band is magnetic thus.In other embodiment of the present invention, needle also can produce magnetic by electromagnetic action.
Needle lifting mechanism comprises that needle promotes end block 17, motor 12, and leading screw 2 32 and nut transmission pair, motor drives promotion end block 17 to move upward by leading screw and nut transmission pair.Needle promotes end block 17 and is arranged on needle support end block 9 belows.Needle promotes end block and can contact with needle support end block.When motor drives when promoting end block and moving upward by leading screw and nut transmission pair, needle promotes end block and contacts with needle support end block, and promotes needle support end block and move upward, thereby promotion needle support moves upward.
Described also have sleeve pipe 4 and sleeve pipe travel mechanism 16 for extracting the instrument of biologically active substance.Sleeve pipe travel mechanism comprises casing support 6 and telescopic lifter structure 18.Casing support comprises casing support end block 35 and casing support arm 5.Diagram the preferred embodiments of the present invention for extracting the instrument of biologically active substance, casing support has two casing support arms that be arranged in parallel.Casing support also can only have a casing support arm, can be also to exceed two.Sleeve pipe is removably fixed on casing support arm.The quantity of the fixable sleeve pipe of each casing support arm can be adjusted as required.The quantity of sleeve pipe can be adjusted as required, and common every group is 1-12 root sleeve pipe.Common is 4,6 or 12 sleeve pipes.At another embodiment, casing support arm can fixed sleeving group, and each sleeve pipe group has many bar casing tubes, 24 sleeve pipes (spread pattern is 4 × 6) of for example corresponding culture plate, 96 sleeve pipes (spread pattern is 8 × 12) etc.
Telescopic lifter structure comprises sleeve pipe lifting end block 36, motor 11, leading screw 1 and nut transmission pair.Motor drives sleeve pipe lifting end block to move up or down by leading screw and nut transmission pair.Sleeve pipe lifting end block is arranged on casing support end block below.Sleeve pipe lifting end block can with the contacting of casing support end block.In the time that motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, sleeve pipe lifting end block can contact with casing support end block, and pushing set tube support end block moves upward, thereby pushing set tube support moves upward.In the time that motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support end block is synchronizeed and is moved downward with sleeve pipe lifting end block according to action of gravity, thereby drives casing support to move downward.
Fig. 3 and Fig. 4 are described for extracting the side cross-sectional schematic of instrument of biologically active substance.Can find out from Fig. 3 and Fig. 4, sleeve pipe lifting end block 36 is separable with casing support end block 35, realizes thus the vibration of sleeve pipe and moves up and down and can be manipulated by different institutions independently.
Sleeve pipe lifting end block is arranged at casing support end block 35 belows.In the time that motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward.In the time that motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support end block is synchronizeed and is moved downward with sleeve pipe lifting end block according to action of gravity, thereby drives casing support to move downward.
Diagram the preferred embodiments of the present invention for extracting the instrument of biologically active substance, as shown in Figure 2 and Figure 5, casing support end block below arranges cover tube vibration push rod 26, it is connected with vibrating mechanism, conduction vibration.
Diagram the preferred embodiments of the present invention for extracting the instrument of biologically active substance, sleeve pipe lifting end block is arranged on casing support end block below, the length of its upper surface is identical with the lower surface of casing support end block with width, can sleeve pipe lifting end block be risen and be supported and pushing effect.In the middle of sleeve pipe lifting end block, there is cavity.Cover tube vibration push rod is also arranged on casing support end block below, and its upper surface length is less than the lower surface of casing support end block with width.In the middle of sleeve pipe lifting end block, have cavity, can allow cover tube vibration push rod pass, the upper end of cover tube vibration push rod contacts with casing support end block, and lower end contacts with the vibrating mechanism that is arranged on sleeve pipe travel mechanism below.
In one aspect of the invention, needle promotion end block 17 is separable with needle support end block 9.Needle promotes a side contacts of end block and needle support end block.In the time that motor drives promotion end block to move upward by leading screw and nut transmission pair, promote needle support by needle support end block and move upward.Can make like this needle upwards from sleeve pipe, depart from.Moving downward of needle support is according to action of gravity.The needle of instrument of the present invention promotes end block and needle support is separable, and this design can realize the abundant close contact of needle termination and sleeve bottom, has simplified the requirement to automatically controlled part, prevents that needle from impacting the phenomenon generation of sleeve bottom.
Fig. 3 illustrates the working order of described instrument in the time that needle inserts sleeve pipe.Needle 4 inserts sleeve pipe, and its bottom contacts with the bottom of sleeve pipe.Now needle promotion end block 17 separates with needle support end block 9, and needle support 8 is supported and together moved with casing support by casing support 6.Another kind of embodiment is that needle 4 inserts sleeve pipe, arrives the position of the predetermined bottom that approaches sleeve pipe most, and now needle promotion end block 17 separates with needle support end block 9, and needle support is supported by casing support and together moves with casing support.Predetermined needle bottom arrive approach most sleeve bottom position can by casing support 6 and needle support 8 arrange limit, for example be set to needle supported arm and casing support arm contact, or needle supported arm contacts with the male member arranging on casing support arm, described male member can be cushioning material preparation.
Fig. 4 illustrates the working order that sleeve pipe separates with needle.Needle promotes a side contacts of end block 17 and needle support end block 9.In the time that motor drives needle promotion end block to move upward by leading screw and nut transmission pair, promote needle support by needle support end block and move upward, needle separates with sleeve pipe.
In addition, sleeve pipe lifting end block 35 contacts with casing support end block 36, and when motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward.In the time that motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support moves downward with sleeve pipe lifting end block under action of gravity.
Thereby in the time needing sleeve pipe by the magnetic bead of the outer absorption of vibration wash-out sleeve pipe or the biological sample with magnetic bead, needle support end block promotion needle support moves upward, and sleeve pipe separates with needle.Sleeve pipe lifting end block and casing support end block disengage.Casing support vibrates under vibrating mechanism effect.
In the time needing sleeve pipe insertion or the centrifuge tube of sample area or the hole of culture plate, sleeve pipe lifting end block 35 contacts with casing support end block 36, when motor drives sleeve pipe lifting end block to move upward by leading screw and nut transmission pair, pushing set tube support moves upward and leaves sample area.In the time that motor drives sleeve pipe lifting end block to move downward by leading screw and nut transmission pair, casing support moves downward with sleeve pipe lifting end block under action of gravity, inserts sample area.
In addition, as shown in the figure, described needle travel mechanism and sleeve pipe travel mechanism are arranged on mechanism's platform 14, are supported by mechanism rack 13.
The both sides of needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35 have perforation, article two, be vertically arranged on the perforation that the optical axis 10 on mechanism's platform passes described needle support end block 9, sleeve pipe lifting end block 36 and casing support end block 35, needle support end block, sleeve pipe lifting end block and casing support end block are risen and limited and fixed action.
The both sides that needle promotes end block also have perforation, are passed by another two optical axises.
Of the present invention have vibrating mechanism for the instrument that extracts biologically active substance, and described vibrating mechanism can produce vibration, makes sleeve pipe travel mechanism drive sleeve pipe to carry out up-down vibration.The sleeve pipe of the instrument of extraction biologically active substance of the present invention can carry out up-down vibration up to the frequency of 20Hz.Described vibrating mechanism of the present invention comprises lever arm, mobile fulcrum and eccentric stiffener, and described mobile fulcrum can move along lever arm.Of the present invention for the instrument that extracts biologically active substance in the course of the work, one of them operation requires the upper and lower fast vibration of needle sheath.According to different job requirements, this vibration will have different amplitudes and frequency.Of the present invention for extract biologically active substance instrument vibrating mechanism be arranged so that vibration amplitude and frequency be adjustable; and anyway amplitude is adjusted; the starting point (lower-most point) of its amplitude is identical consistent; thereby reach the object of the mechanisms such as protection sheath; together with further feature of the present invention; make instrument of the present invention there is low noise, the advantage of high life.
As shown in Figure 5, driving mechanism of the present invention comprises linear lever arm 22, traversing carriage 23, mobile fulcrum shaft 37, the driving mechanism 24 that drives traversing carriage to move horizontally, contact and the vibrating wheels 25 of conduction vibration with the cover tube vibration push rod 26 of telescopic lifter structure, and eccentric wheel 21 and eccentric wheel drive-motor 20.Lever arm is linear pattern, has the hollow groove parallel with lower edge with its upper limb.Mobile fulcrum shaft, through hollow groove, can move along lever arm direction under traversing carriage driving mechanism drives in hollow groove, forms the mobile fulcrum of lever.Contact with the cover tube vibration push rod 26 of telescopic lifter structure and the vibrating wheels 25 of conduction vibration and eccentric wheel lay respectively at the both sides of mobile fulcrum.Eccentric wheel contacts with lever arm, when eccentric wheel rotates, cause at the lever arm (power arm) of eccentric wheel one side of fulcrum and be subjected to displacement, the lever arm (resistance arm) of the opposite side of fulcrum and vibrating wheels are synchronously carried out rightabout displacement, and the rotation around fulcrum occurs lever arm.Eccentric rotation makes vibrating wheels vibrate, thereby makes sleeve pipe travel mechanism drive sleeve pipe to carry out up-down vibration.Also, of the present invention for extracting the vibrating mechanism of instrument of biologically active substance, produce vibration, the speed of controlled frequency by eccentric stiffener (being eccentric wheel in embodiment).The size of the controlled damping width of movement of the position of mobile fulcrum on lever arm also can be controlled the size of amplitude together with eccentric rotating manner.
In the instrument of the preferred embodiments of the present invention shown in Fig. 5, eccentric wheel directly contacts with linear pattern lever arm, eccentric minimum radius (being the minor increment that wheel rim is arrived in axle center) equates to the distance of lever arm with its axle center, the travel direction (being lever arm) that the radial direction of eccentric wheel minimum radius and lever move fulcrum is vertical, now, no matter how fulcrum moves in the hollow groove of lever arm, in the time of the contacting of eccentric wheel and lever arm generation minimum radius, vibrating wheels is the lower-most point in its amplitude all.No matter how eccentric rotating speed changes, no matter also how the position of mobile fulcrum on lever arm changes,, regardless of amplitude and the frequency of vibrating wheels, the lower-most point of vibrating wheels is all constant, and the lower-most point that namely sleeve pipe occurs to vibrate is constant.
In other embodiment of the present invention, also can replace eccentric wheel to realize object of the present invention with cam.
The instrument of extraction biologically active substance of the present invention is by adopting above-mentioned vibrating mechanism, can adjust arbitrarily vibrational frequency and amplitude, no matter how amplitude is adjusted simultaneously, the minimum start position of various amplitude is all identical, can increase substantially the setting range of vibrational frequency, make whole instrument there is low noise, the advantage of high life.
Although describe in detail about example embodiment of the present invention and advantage thereof, be to be understood that the protection domain in the case of not departing from spirit of the present invention and claims restriction, can carry out various variations, substitutions and modifications to these embodiment.For other examples, those of ordinary skill in the art should easily understand in keeping in protection domain of the present invention, and the order of processing step can change.
In addition, range of application of the present invention is not limited to technique, mechanism, manufacture, material composition, means, method and the step of the specific embodiment of describing in specification sheets.From disclosure of the present invention, to easily understand as those of ordinary skill in the art, for had or be about at present technique, mechanism, manufacture, material composition, means, method or the step developed later, wherein they carry out identical function or the identical result of acquisition cardinal principle of corresponding embodiment cardinal principle of describing with the present invention, can apply them according to the present invention.Therefore, claims of the present invention are intended to these technique, mechanism, manufacture, material composition, means, method or step to be included in its protection domain.
Claims (10)
1. for extracting an instrument for biologically active substance, preferred, it is for for extracting and the instrument of purification of nucleic acid, and described instrument comprises:
Shell and base plate (1),
Worktable (15), has sample preparation district on it,
Needle (7) and needle travel mechanism (48), needle travel mechanism comprises needle support (8) and needle lifting mechanism (33), needle is removably fixed on needle support, wherein, by moving up and down of needle lifting mechanism, make needle can vertically insert and depart from sleeve pipe
Sleeve pipe (4) and sleeve pipe travel mechanism (16), sleeve pipe travel mechanism comprises casing support (6) and telescopic lifter structure (18), sleeve pipe is removably fixed on casing support, wherein, by moving up and down of telescopic lifter structure, make sleeve pipe vertically to insert and to depart from sample preparation district, in addition, sleeve pipe travel mechanism (16) can up-down vibration;
Be used for supporting the mechanism rack (13) of described needle travel mechanism (48) and sleeve pipe travel mechanism (16),
With
Vibrating mechanism (19), described vibrating mechanism can produce and export vibration, makes sleeve pipe travel mechanism (16) carry out up-down vibration, wherein,
Vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produce vibration, it is characterized in that, wherein said lever arm, being set to of movable fulcrum and rotating mechanism, in working order, the variation of the position of movable fulcrum is limited to lowest amplitude position of the fulcrum, lowest amplitude position of the fulcrum refers to, when the nearest profile point of the described rotating mechanism of vibrating mechanism produces pushing effect to lever arm, the position of the balance pivot that in vibrating mechanism, lever arm rotates about it.
2. instrument claimed in claim 1, wherein said rotating mechanism is eccentric rotary mechanism, Bu center, its axle center, for example, described eccentric rotary mechanism is eccentric wheel or the cam with non-central axle center.
3. the instrument described in claim 1 or 2, wherein said movable fulcrum is the mobile fulcrum that can move or be arranged on groove on lever arm or hollow groove and move along the lower edge of lever arm.
4. the instrument described in claim 1 or 2, wherein said lever arm is linear pattern lever arm, preferred, it has the groove parallel with upper surface or hollow groove, can be for contacting with fulcrum.
5. instrument claimed in claim 1, wherein said vibrating mechanism comprises linear pattern lever arm (22), mobile fulcrum and eccentric wheel (21), mobile fulcrum can move along lever arm, its travel direction is the axial direction due of lever arm, and wherein eccentric minimum radius equates to the distance of lever arm with its axle center.
6. the instrument described in claim 1 or 2, wherein needle support (8) comprises needle support end block (9) and needle supported arm (34), needle is removably fixed on needle supported arm; Described needle lifting mechanism comprises that the needle that is arranged on needle support end block below promotes end block (17), when needle promotion end block moves upward, promoting needle support end block moves upward, thereby promoting needle support moves upward, wherein needle promotion end block (17) is separable with needle support (8), when needle support contacts with casing support in the process declining, needle promotes end block and separates with needle support, and needle support is supported by casing support and together moves with casing support.
7. the instrument described in claim 1 or 2, wherein casing support (6) comprises casing support end block (35) and casing support arm (5), sleeve pipe is removably fixed on casing support arm; Described telescopic lifter structure comprises the sleeve pipe lifting end block (36) that is arranged on casing support end block below, when sleeve pipe lifting end block moves upward, pushing set tube support end block moves upward, thereby pushing set tube support moves upward, when lifting end block moves downward, casing support end block is synchronizeed and is moved downward with sleeve pipe lifting end block according to action of gravity, and wherein said sleeve pipe lifting end block and casing support are separable.
8. the instrument extraction described in any one and the method for purifying biological active substance in a right to use requirement 1-7.
9. the vibrating mechanism for biologically active substance extraction apparatus, described extraction apparatus has needle and needle travel mechanism, and sleeve pipe and sleeve pipe travel mechanism, by moving up and down of needle travel mechanism, make needle can vertically insert and depart from sleeve pipe, by moving up and down of sleeve pipe travel mechanism, make sleeve pipe can vertically insert and depart from the sample preparation district of its below, in addition, described vibrating mechanism can produce vibration, drive described sleeve pipe up-down vibration
It is characterized in that, described vibrating mechanism comprises lever arm, movable fulcrum and rotating mechanism, wherein said rotating mechanism has wheel disc and axle center, and when described rotating mechanism rotates around axle center, the outer rim of its wheel disc can promote lever arm and rotate around movable fulcrum, thereby produce vibration
Preferably, described vibrating mechanism has the feature as described in any one in claim 1-7.
A right to use require vibrating mechanism described in 9 in bar magnet method biologically active substance extraction apparatus to the vibrative method of the sleeve pipe of extraction apparatus.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310014908.3A CN103923831B (en) | 2013-01-15 | 2013-01-15 | For extracting the instruments and methods of biologically active substance |
| HK15100445.4A HK1199902A1 (en) | 2013-01-15 | 2015-01-15 | Instrument and method for extraction of bioactive substances |
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| CN201310014908.3A CN103923831B (en) | 2013-01-15 | 2013-01-15 | For extracting the instruments and methods of biologically active substance |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527150A (en) * | 2015-12-10 | 2016-04-27 | 南京医科大学 | High-throughput magnetic extraction enrichment device and enrichment method |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN109266547A (en) * | 2018-11-27 | 2019-01-25 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
| CN111699242A (en) * | 2017-10-11 | 2020-09-22 | 上海仁度生物科技有限公司 | Automated nucleic acid sample preparation, detection and analysis system |
| CN114366613A (en) * | 2022-01-30 | 2022-04-19 | 宁波市第一医院 | Partitioned stone discharging bed and working method thereof |
| WO2022174470A1 (en) * | 2021-02-19 | 2022-08-25 | 杭州梓晶生物有限公司 | Fully integrated pathogen nucleic acid analyzer |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106381259B (en) * | 2016-09-27 | 2018-09-11 | 深圳市港科深研生物科技有限公司 | The vitro extraction device of peripheral blood mononuclear cells and the vitro extraction method of peripheral blood mononuclear cells |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030082565A1 (en) * | 2001-10-31 | 2003-05-01 | Jang Gi Young | Method of manufacturing kit for isolating nucleic acids or biological materials, kit manufactured by the method, and apparatus using the kit |
| CN2890059Y (en) * | 2006-01-24 | 2007-04-18 | 陈晓汀 | Nucleic acid extracting instrument |
| CN101143884A (en) * | 2007-10-15 | 2008-03-19 | 吉林大学 | Nucleic acid automatic extracting instrument |
| US20080241914A1 (en) * | 2003-01-29 | 2008-10-02 | Hee Joun Roh | System for automatically performing separation or purification of biological materials |
| CN201510887U (en) * | 2009-05-14 | 2010-06-23 | 上海科华实验系统有限公司 | Liquid-transferring device with an automatic imbibition head removing mechanism |
| CN203174103U (en) * | 2013-01-15 | 2013-09-04 | 北京金麦格生物技术有限公司 | Instrument for extracting bioactive substance |
-
2013
- 2013-01-15 CN CN201310014908.3A patent/CN103923831B/en not_active Expired - Fee Related
-
2015
- 2015-01-15 HK HK15100445.4A patent/HK1199902A1/en unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030082565A1 (en) * | 2001-10-31 | 2003-05-01 | Jang Gi Young | Method of manufacturing kit for isolating nucleic acids or biological materials, kit manufactured by the method, and apparatus using the kit |
| US20080241914A1 (en) * | 2003-01-29 | 2008-10-02 | Hee Joun Roh | System for automatically performing separation or purification of biological materials |
| CN2890059Y (en) * | 2006-01-24 | 2007-04-18 | 陈晓汀 | Nucleic acid extracting instrument |
| CN101143884A (en) * | 2007-10-15 | 2008-03-19 | 吉林大学 | Nucleic acid automatic extracting instrument |
| CN201510887U (en) * | 2009-05-14 | 2010-06-23 | 上海科华实验系统有限公司 | Liquid-transferring device with an automatic imbibition head removing mechanism |
| CN203174103U (en) * | 2013-01-15 | 2013-09-04 | 北京金麦格生物技术有限公司 | Instrument for extracting bioactive substance |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105527150A (en) * | 2015-12-10 | 2016-04-27 | 南京医科大学 | High-throughput magnetic extraction enrichment device and enrichment method |
| CN105527150B (en) * | 2015-12-10 | 2018-10-23 | 南京医科大学 | A kind of high-throughput magnetic extracting and enriching device and enrichment method |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN111699242A (en) * | 2017-10-11 | 2020-09-22 | 上海仁度生物科技有限公司 | Automated nucleic acid sample preparation, detection and analysis system |
| CN109266547A (en) * | 2018-11-27 | 2019-01-25 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
| WO2022174470A1 (en) * | 2021-02-19 | 2022-08-25 | 杭州梓晶生物有限公司 | Fully integrated pathogen nucleic acid analyzer |
| CN114366613A (en) * | 2022-01-30 | 2022-04-19 | 宁波市第一医院 | Partitioned stone discharging bed and working method thereof |
| CN114366613B (en) * | 2022-01-30 | 2024-04-19 | 宁波市第一医院 | Partition stone removing bed and working method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1199902A1 (en) | 2015-07-24 |
| CN103923831B (en) | 2015-09-16 |
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