CN109266547A - Nucleic acid-extracting apparatus - Google Patents
Nucleic acid-extracting apparatus Download PDFInfo
- Publication number
- CN109266547A CN109266547A CN201811425934.4A CN201811425934A CN109266547A CN 109266547 A CN109266547 A CN 109266547A CN 201811425934 A CN201811425934 A CN 201811425934A CN 109266547 A CN109266547 A CN 109266547A
- Authority
- CN
- China
- Prior art keywords
- bar magnet
- connecting plate
- frame
- slider connecting
- guide rail
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000758 substrate Substances 0.000 claims abstract description 26
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000007246 mechanism Effects 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 abstract description 16
- 108020004707 nucleic acids Proteins 0.000 abstract description 16
- 150000007523 nucleic acids Chemical class 0.000 abstract description 16
- 238000000605 extraction Methods 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 8
- 239000011324 bead Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000005336 cracking Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007859 qualitative PCR Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to nucleic acid-extracting apparatus technical fields, provide a kind of nucleic acid-extracting apparatus, comprising: pedestal, substrate, bottom plate, frame, bar magnet frame and bar magnet stock；The substrate is rotatably installed in the surface of the pedestal, the bottom plate is mounted on the surface of the substrate, the frame is mounted on the bottom plate, the bar magnet frame is slidably mounted on the frame, and the bar magnet arranged vertically is installed on the bar magnet frame, the bar magnet stock is slidably mounted on the frame, and the bar magnet stock is located at the underface of the bar magnet frame, and the bar magnet stock is for installing bar magnet set.The nucleic acid extraction of the nucleic acid-extracting apparatus is high-efficient, running precision is high, stability is good.
Description
Technical field
The present invention relates to nucleic acid-extracting apparatus technical fields, more particularly to a kind of nucleic acid-extracting apparatus.
Background technique
In genetic engineering and protein engineering research field, nucleic acid molecules are main study subjects, and point of nucleic acid
From the basic fundamental for linearisation being nucleic acids research, nucleic acid is the basic unit for representing life entity hereditary feature, and detection of nucleic acids is
Advanced biological detection is carried out on a molecular scale, compared to traditional morphologic detection, cytology detection and immunology detection etc.
The more remarkable advantages such as sensitivity, specificity, without window phase, detection of nucleic acids include that qualitative PCR, molecule hybridization and real-time fluorescence are fixed
Measure the technologies such as PCR, the key of these nucleic acid detection techniques be to complete to extract the nucleic acid of biological sample, therefore effectively and
Accurately extract the nucleic acid-templated premise for becoming subsequent detection of nucleic acids.
Currently, domestic nucleic acid extraction mainly uses traditional craft preparation method, not only inefficiency is at high cost, Er Qiechong
Renaturation and stability are all bad.
Summary of the invention
(1) technical problems to be solved
The object of the present invention is to provide a kind of nucleic acid-extracting apparatus, it is intended at least solve the prior art or deposit in the related technology
The technical issues of one of.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of nucleic acid-extracting apparatus, comprising: pedestal, substrate, bottom plate,
Frame, bar magnet frame and bar magnet stock；The substrate is rotatably installed in the surface of the pedestal, and the bottom plate is mounted on
The surface of the substrate, the frame are mounted on the bottom plate, and the bar magnet frame is slidably mounted on the frame,
And the bar magnet arranged vertically is installed on the bar magnet frame, the bar magnet stock is slidably mounted on the frame, and institute
The underface that bar magnet stock is located at the bar magnet frame is stated, the bar magnet stock is for installing bar magnet set.
It wherein, further include rotating mechanism, the rotating mechanism includes bearing, driving gear, driven gear and the first drive
Dynamic motor；The pedestal is connect with the inner ring of the bearing, and the outer ring of the bearing is connect with the substrate, and the bearing
Outer ring is arranged with the driven gear, is equipped with first driving motor on the substrate, first driving motor it is defeated
The driving gear is installed, the driving gear is meshed with the driven gear on shaft.
Wherein, the bottom plate is connect by several fixed columns with the substrate.
It wherein, further include sliding equipment, the sliding equipment includes that the first guide rail, the first slider connecting plate and second are sliding
Block connecting plate；On said frame, first slider connecting plate and second sliding block connect the first guide rail right angle setting
Groove compatible with first guide rail is provided on fishplate bar, first slider connecting plate is for installing the bar magnet
Frame, second slider connecting plate is for installing the bar magnet stock.
Wherein, the sliding equipment further includes the second guide rail, third slider connecting plate and Four-slider connecting plate, described
On said frame, and first guide rail and second guide rail install the frame two to second guide rail right angle setting respectively
On opposite side, it is provided on the third slider connecting plate and the Four-slider connecting plate and the second guide rail phase
The groove of adaptation, the third slider connecting plate is for installing the bar magnet frame, and the Four-slider connecting plate is for installing institute
State bar magnet stock.
Wherein, bar magnet motor mount is installed between second slider connecting plate and the Four-slider connecting plate,
First bar magnet motor and the second bar magnet motor are installed, the first bar magnet motor is for driving on the bar magnet motor mount
First slider connecting plate carries out straight reciprocating motion and provides vibration force for first slider connecting plate, and described second
Bar magnet motor is for driving the third slider connecting plate to carry out straight reciprocating motion and mention for the third slider connecting plate
For vibration force, the second driving motor is also equipped on the frame, second driving motor is used for while driving described second
Slider connecting plate and the Four-slider connecting plate carry out straight reciprocating motion.
It wherein, further include sliding equipment, the sliding equipment is slided including the first guide rail, the first sliding block, the second sliding block, first
Block connecting plate and the second slider connecting plate, the first guide rail right angle setting on said frame, first sliding block and institute
It states and is provided with groove compatible with first guide rail on the second sliding block, first sliding block is connected with first sliding block
Plate connection, second sliding block are connected with second slider connecting plate, and first slider connecting plate is for installing the magnetic
Stick frame, second slider connecting plate is for installing the bar magnet stock.
Wherein, the sliding equipment include also the second guide rail, third sliding block, Four-slider, third slider connecting plate and
Four-slider connecting plate, the second guide rail right angle setting on said frame, and first guide rail and second guide rail
It installs on two opposite sides of the frame, is provided on the third sliding block and the Four-slider and described respectively
The compatible groove of two guide rails, the third sliding block are connected with the third slider connecting plate, the Four-slider and described
The connection of Four-slider connecting plate, for installing the bar magnet frame, the Four-slider connecting plate is used for the third slider connecting plate
The bar magnet stock is installed.
Wherein, the bar magnet frame includes bar magnet connecting plate and magnetic pole frame, and there are three described for connection on the bar magnet connecting plate
Magnetic pole frame, the magnetic pole frame is for installing bar magnet.
Wherein, the bar magnet stock includes bar magnet set connecting plate and bar magnet set mounting rack, is connected on bar magnet set connecting plate
Three bar magnets cover mounting rack, and the bar magnet set mounting rack is for installing bar magnet set.
(3) beneficial effect
Compared with prior art, the invention has the following advantages that
A kind of nucleic acid-extracting apparatus provided by the invention, substrate are rotatably installed in the surface of pedestal, bottom plate installation
In the surface of substrate, frame is mounted on bottom plate, and bar magnet frame is slidably mounted on frame, and is equipped on bar magnet frame perpendicular
The bar magnet directly arranged, bar magnet stock are slidably mounted on frame, and bar magnet stock is located at the underface of bar magnet frame, bar magnet set
Frame is for installing bar magnet set.It can accurately realize the relative motion between bar magnet and bar magnet set, the nucleic acid of the nucleic acid-extracting apparatus
Extraction efficiency is high, running precision is high, stability is good.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of nucleic acid-extracting apparatus of the embodiment of the present invention；
Fig. 2 is the left view of Fig. 1；
Fig. 3 is the right view of Fig. 1；
Fig. 4 is a kind of partial enlarged view of nucleic acid-extracting apparatus of the embodiment of the present invention；
Fig. 5 is the structural schematic diagram of sliding equipment in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention；
Fig. 6 is the structural schematic diagram of bar magnet frame in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention；
Fig. 7 is the structural schematic diagram of bar magnet stock in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention；
In figure: 1- frame；2- bar magnet frame；211- bar magnet connecting plate；212- magnetic pole frame；3- bar magnet stock；311- bar magnet set
Connecting plate；312- bar magnet covers mounting rack；4- bottom plate；5- substrate；6- pedestal；The first driving motor of 7-；8- driving gear；9- is driven
Gear；10- bearing；11- fixed column；12- third slider connecting plate；13- Four-slider connecting plate；The second slider connecting plate of 14-；
The first slider connecting plate of 15-；16- bar magnet motor mount；17- nut；18- the first bar magnet motor；The second driving motor of 19-；
The first guide rail of 20-；The first sliding block of 21-；22- bar magnet.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiments of the present invention will be described in further detail.Following instance
For illustrating the present invention, but it is not intended to limit the scope of the invention.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on the figure or
Positional relationship is merely for convenience of description of the present invention and simplification of the description, rather than the device or element of indication or suggestion meaning must
There must be specific orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the present invention, the meaning of " plurality " is at least two, such as two, three
It is a etc., unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；It can be mechanical connect
It connects, is also possible to be electrically connected or can communicate each other；It can be directly connected, can also indirectly connected through an intermediary, it can be with
It is the interaction relationship of the connection or two elements inside two elements, unless otherwise restricted clearly.For this field
For those of ordinary skill, the specific meanings of the above terms in the present invention can be understood according to specific conditions.
Fig. 1 is a kind of structural schematic diagram of nucleic acid-extracting apparatus of the embodiment of the present invention, and Fig. 2 is the left view of Fig. 1, and Fig. 3 is
The right view of Fig. 1；As shown in Figure 1, Figure 2 and Figure 3, nucleic acid-extracting apparatus provided in an embodiment of the present invention includes: pedestal 6, substrate
5, bottom plate 4, frame 1, bar magnet frame 2 and bar magnet stock 3；Substrate 5 is rotatably installed in the surface of pedestal 6, and bottom plate 4 is installed
In the surface of substrate 5, frame 1 is mounted on bottom plate 4, and bar magnet frame 2 is slidably mounted on frame 1, and is pacified on bar magnet frame 2
Equipped with the bar magnet 22 arranged vertically, bar magnet stock 3 is slidably mounted on frame 1, and bar magnet stock 3 is located at bar magnet frame 2
Underface, bar magnet stock 3 is for installing bar magnet set.
At initial position, bar magnet frame 2 is separated with bar magnet stock 3, and with the operation that is mounted on bottom plate
Platform (not shown) is sufficiently spaced-apart big distance.Nucleic acid-extracting apparatus provided by the invention is successively run according to the following steps.
The first step, sample-adding-- are put into the deep-well plates after addition sample the specified location of station, then, bar magnet are covered and is fixed
To the conduit of bar magnet stock 3.Second step, cracking-- make bar magnet stock 3 drive bar magnet set downlink, until bar magnet set enters deep hole
In the hole of the specified row of plate, that one end that bar magnet covers separate bar magnet stock 3 twists in the hole of deep-well plates mixes sample, lysate and magnetic
The mixture of pearl (being prepositioned in the hole of deep-well plates), makes the nucleic acid in sample separate (i.e. nucleic acid from sample
It is cleaved), and be adsorbed on magnetic bead.Third step, absorption-- bar magnet frame 2 drive bar magnet downlink, until bar magnet 22 enters
The designated position of bar magnet set, stands the predetermined time, and then, bar magnet frame 2 and bar magnet 22 are together by the pre- timing of preset frequency vibration
Between, so that whole magnetic beads is attracted to the outer surface of bar magnet set, then, bar magnet frame out together with the nucleic acid for cracking in sample
2 and bar magnet set uplink makes bar magnet set and bar magnet 22 together into being washed in the washing hole of the specified row of deep-well plates again together,
To remove impurity.4th step, elution-- bar magnet stock 3 and bar magnet set are entered in elution hole by washing hole together, are being eluted
Under the action of liquid, nucleic acid is released from magnetic bead.5th step, recycling magnetic bead-- will be adsorbed on the magnetic of the outer surface of bar magnet set
Pearl is transferred in magnetic bead recovery holes by eluting hole, and bar magnet 22 leaves bar magnet set.6th step is abandoned magnetic-- bar magnet and is covered in magnetic bead recycling
Hole internal vibration makes magnetic bead all fall off from the outer surface that bar magnet covers.7th step, cleaning-- unload bar magnet set, remove deep-well plates,
Bar magnet frame 2 and bar magnet stock 3 return to initial position.Wherein, it in cracking, elution step, needs to mix in the sample in deep-well plates
It closes liquid and is heated to predetermined temperature range.
As shown in Figures 2 and 3, which further includes rotating mechanism, and rotating mechanism includes bearing 10, driving tooth
Wheel 8, driven gear 9 and the first driving motor 7；Pedestal 6 is connect with the inner ring of bearing 10, and the outer ring of bearing 10 and substrate 5 connect
Connect, and the outer ring of bearing 10 is arranged with driven gear 9, the first driving motor 7 be installed on substrate 5, the first driving motor 7 it is defeated
Driving gear 8 is installed, driving gear 8 is meshed with driven gear 9 on shaft.
Wherein, lug boss is provided on pedestal 6, bearing 10 is set on lug boss, and the first driving motor 7 is electric rotating
Machine.
Further, bottom plate 4 is connect by several fixed columns 11 with substrate 5.
Wherein, bottom plate 4 is connect by several fixed columns 11 with substrate 5, enables to exist between bottom plate 4 and substrate 5
Mounting rotary electric machine is carried out in certain space.
Fig. 5 is the structural schematic diagram of sliding equipment in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention, as shown in figure 5, should
Nucleic acid-extracting apparatus further includes sliding equipment, and sliding equipment is sliding including the first guide rail 20, the first slider connecting plate 15 and second
Block connecting plate 14；First guide rail 20 is vertically mounted on frame 1, on the first slider connecting plate 15 and the second slider connecting plate 14
It is provided with groove compatible with the first guide rail 20, the first slider connecting plate 15 is for installing bar magnet frame 2, the second slider connecting plate
14 for installing bar magnet stock 3.
In the present embodiment, two first guide rails 20, two first guide rails 20 are separately installed on a side of frame 1
Between be spaced a distance, two first guide rails 20 parallel arrangement, and being arranged along the short transverse of frame 1.First sliding block connects
The both ends of fishplate bar 15 are respectively arranged with groove compatible with the first guide rail 20, and the both ends of the second slider connecting plate 14 are also set respectively
It is equipped with groove compatible with the first guide rail 20, the first slider connecting plate 15 is located at the surface of the second slider connecting plate 14, the
One slider connecting plate 15 is for installing bar magnet frame 2, and the second slider connecting plate 14 is for installing bar magnet stock 3.
As shown in figure 4, sliding equipment further includes the second guide rail (not shown), third slider connecting plate 12 and the 4th
Slider connecting plate 13, the second guide rail are vertically mounted on frame, and the first guide rail 20 and the second guide rail distinguish installation frame 1 two
On opposite side, it is provided on third slider connecting plate 12 and Four-slider connecting plate 13 compatible with the second guide rail recessed
Slot, third slider connecting plate 12 is for installing bar magnet frame 2, and Four-slider connecting plate 13 is for installing bar magnet stock 3.
In the present embodiment, be separately installed with two second guide rails on another side of frame 1, two second guide rails it
Between be spaced a distance, two second guide rails parallel arrangement, and being arranged along the short transverse of frame 1.Third slider connecting plate
12 both ends are respectively arranged with groove compatible with the second guide rail, the both ends of Four-slider connecting plate 13 be also respectively arranged with
The compatible groove of second guide rail, third slider connecting plate 12 are located at the surface of Four-slider connecting plate 13, and third sliding block connects
Fishplate bar 12 is for installing bar magnet frame 2, and Four-slider connecting plate 13 is for installing bar magnet stock 3.
Wherein, the first guide rail 20 is identical as the specification of the second guide rail, the first slider connecting plate 15, the second slider connecting plate
14, the specification of third slider connecting plate 12 and Four-slider connecting plate 13 is identical.
Further, bar magnet motor mount is installed between the second slider connecting plate 14 and Four-slider connecting plate 13
16, the first bar magnet motor 18 and the second bar magnet motor (not shown), the first bar magnet are installed on bar magnet motor mount 16
Motor 18 is for driving the first slider connecting plate 15 to carry out straight reciprocating motion and providing vibration for the first slider connecting plate 15
Power, the second bar magnet motor is for driving the progress straight reciprocating motion of third slider connecting plate 12 and being third slider connecting plate 12
Vibration force is provided, the second driving motor 19 is also equipped on frame 1, the second driving motor 19 is used for while the second sliding block being driven to connect
Fishplate bar 14 and Four-slider connecting plate 13 carry out straight reciprocating motion.
Wherein, the second driving motor 19 is Z axis motor, and the output end of the second driving motor 19 is equipped with nut 17, nut
It is connect with bar magnet motor mount 16.The output end of first bar magnet motor 18 is connect with the first slider connecting plate 15, the second bar magnet
The output end of motor is connect with third slider connecting plate 12.First bar magnet motor 18 and the second bar magnet motor are separately mounted to bar magnet
The both ends on the same surface of motor mount 16, one end of bar magnet motor mount 16 are connect with the second slider connecting plate 14, bar magnet
The other end of motor mount 16 is connect with Four-slider connecting plate 13.
In the present embodiment, the second driving motor 19 works, when the first bar magnet motor 18 and the second bar magnet motor do not work,
Second driving motor 19 can be by driving bar magnet motor mount 16 to pump, 16 band of bar magnet motor mount
Dynamic the second slider connecting plate 14 being attached thereto and Four-slider connecting plate 13 pump, and then drive first to slide
Block connecting plate 15 and third slider connecting plate 12 pump, the first slider connecting plate 15, the second slider connecting plate
14, third slider connecting plate 12 and Four-slider connecting plate 13 synchronize pump；The work of first bar magnet motor 18
When, the first slider connecting plate 15 can be driven to carry out straight reciprocating motion and provide vibration force for the first slider connecting plate 15；
When second bar magnet motor works, third slider connecting plate 12 can be driven to carry out straight reciprocating motion and connect for third sliding block
Plate 12 provides vibration force.
The present invention implements another nucleic acid-extracting apparatus provided, and the difference of the two is the difference of sliding equipment, slides
Mechanism is sliding including the first guide rail 20, the first sliding block 21, the second sliding block (not shown), the first slider connecting plate 15 and second
Block connecting plate 14, the first guide rail 20 are vertically mounted on frame 1, are provided on the first sliding block 21 and the second sliding block and are led with first
The compatible groove of rail 20, the first sliding block 21 and the connection of the first slider connecting plate 15, the second sliding block and the second slider connecting plate 14
Connection, the first slider connecting plate 15 is for installing bar magnet frame 2, and the second slider connecting plate 14 is for installing bar magnet stock 3.
In the present embodiment, two first guide rails 20, two first guide rails 20 are separately installed on a side of frame 1
Between be spaced a distance, two first guide rails 20 parallel arrangement, and being arranged along the short transverse of frame 1.Including two
One sliding block 21, the first sliding block 2 are provided with groove compatible with the first guide rail 20,15 one end of the first slider connecting plate with one the
The connection of one sliding block 21,15 other end of the first slider connecting plate are connect with another first sliding block 21；Including two the second sliding blocks,
Two sliding blocks are provided with groove compatible with the first guide rail 20, and 14 one end of the second slider connecting plate is connect with second sliding block,
Second slider connecting plate, 14 other end is connect with another second sliding block.First slider connecting plate 15 is located at the second slider connecting plate
14 surface, the first slider connecting plate 15 is for installing bar magnet frame 2, and the second slider connecting plate 14 is for installing bar magnet stock 3.
Further, sliding equipment includes going back the second guide rail (not shown), third sliding block (not shown), the 4th
Sliding block (not shown), third slider connecting plate 12 and Four-slider connecting plate 13, the second guide rail are vertically mounted on frame 1
On, and on the first guide rail 20 and the second guide rail difference two opposite sides of installation frame, on third sliding block and Four-slider
It is provided with groove compatible with the second guide rail, third sliding block and third slider connecting plate 12 connect, and Four-slider and the 4th is slided
Block connecting plate 13 connects, and third slider connecting plate 12 is for installing bar magnet frame 2, and Four-slider connecting plate 13 is for installing bar magnet set
Frame 3.
In the present embodiment, be separately installed with two second guide rails on another side of frame 1, two second guide rails it
Between be spaced a distance, two second guide rails parallel arrangement, and being arranged along the short transverse of frame 1.It is sliding including two thirds
Block, third sliding block are provided with groove compatible with the second guide rail, and 12 one end of third slider connecting plate connects with a third sliding block
It connects, 12 other end of third slider connecting plate is connect with another third sliding block；Including two Four-sliders, Four-slider is provided with
Groove compatible with the second guide rail, 13 one end of Four-slider connecting plate are connect with a Four-slider, Four-slider connecting plate
13 other ends are connect with another Four-slider.Third slider connecting plate 12 is located at the surface of Four-slider connecting plate 13, the
Three slider connecting plates 12 are for installing bar magnet frame 2, and Four-slider connecting plate 13 is for installing bar magnet stock 3.
Wherein, the first guide rail 20 is identical as the specification of the second guide rail, the first slider connecting plate 15, the second slider connecting plate
14, the specification of third slider connecting plate 12 and Four-slider connecting plate 13 is identical.First sliding block 21, the second sliding block, third are sliding
The specification of block and Four-slider is identical.
Fig. 6 is the structural schematic diagram of bar magnet frame in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention, as shown in fig. 6, bar magnet
Frame 2 includes bar magnet connecting plate 211 and magnetic pole frame 212, and there are three magnetic pole frame 212, magnetic pole framves 212 for connection on bar magnet connecting plate 211
For installing bar magnet 22.
In the present embodiment, it is illustrated so that bar magnet connecting plate 211 is connect with the first slider connecting plate 15 as an example, magnetic pole frame
212 be rectangular shaped rim, and the side frame of magnetic pole frame 212 is connect with bar magnet connecting plate 211.
Fig. 7 is the structural schematic diagram of bar magnet stock in a kind of nucleic acid-extracting apparatus of the embodiment of the present invention, as shown in fig. 7, magnetic
Stick stock 3 includes bar magnet set connecting plate 311 and bar magnet covers mounting rack 312, and bar magnet covers connection on connecting plate 311, and there are three bar magnet sets
Mounting rack 312, bar magnet set mounting rack 312 is for installing bar magnet set (not shown).
In the present embodiment, bar magnet covers mounting rack 312 to the conduit having with bar magnet number of rows corresponding number.Each conduit
A bar magnet set can be inserted.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of nucleic acid-extracting apparatus characterized by comprising pedestal, substrate, bottom plate, frame, bar magnet frame and bar magnet set
Frame；The substrate is rotatably installed in the surface of the pedestal, and the bottom plate is mounted on the surface of the substrate, described
Frame is mounted on the bottom plate, and the bar magnet frame is slidably mounted on the frame, and is equipped on the bar magnet frame
The bar magnet arranged vertically, the bar magnet stock are slidably mounted on the frame, and the bar magnet stock is located at the magnetic
The underface of stick frame, the bar magnet stock is for installing bar magnet set.
2. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that it further include rotating mechanism, the rotating mechanism packet
Include bearing, driving gear, driven gear and the first driving motor；The pedestal is connect with the inner ring of the bearing, the axis
The outer ring held is connect with the substrate, and the outer ring of the bearing is arranged with the driven gear, installs on the substrate
The first driving motor is stated, the driving gear, the driving gear and institute are installed on the output shaft of first driving motor
Driven gear is stated to be meshed.
3. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that the bottom plate by several fixed columns with it is described
Substrate connection.
4. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that it further include sliding equipment, the sliding equipment packet
Include the first guide rail, the first slider connecting plate and the second slider connecting plate；The first guide rail right angle setting on said frame,
Groove compatible with first guide rail, institute are provided on first slider connecting plate and second slider connecting plate
The first slider connecting plate is stated for installing the bar magnet frame, second slider connecting plate is for installing the bar magnet stock.
5. nucleic acid-extracting apparatus as claimed in claim 4, which is characterized in that the sliding equipment further includes the second guide rail,
Three slider connecting plates and Four-slider connecting plate, on said frame, and described first leads the second guide rail right angle setting
Rail and second guide rail are installed respectively on two opposite sides of the frame, the third slider connecting plate and the described 4th
Groove compatible with second guide rail is provided on slider connecting plate, the third slider connecting plate is described for installing
Bar magnet frame, the Four-slider connecting plate is for installing the bar magnet stock.
6. nucleic acid-extracting apparatus as claimed in claim 5, which is characterized in that second slider connecting plate and the described 4th is slided
Bar magnet motor mount is installed between block connecting plate, the first bar magnet motor and second are installed on the bar magnet motor mount
Bar magnet motor, the first bar magnet motor is for driving the first slider connecting plate progress straight reciprocating motion and being described
First slider connecting plate provides vibration force, and the second bar magnet motor is past for driving the third slider connecting plate to carry out straight line
It is multiple to move and provide vibration force for the third slider connecting plate, the second driving motor is also equipped on the frame, it is described
Second driving motor is used for while driving second slider connecting plate and the Four-slider connecting plate to carry out linear reciprocation fortune
It is dynamic.
7. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that it further include sliding equipment, the sliding equipment packet
The first guide rail, the first sliding block, the second sliding block, the first slider connecting plate and the second slider connecting plate are included, first guide rail hangs down
Straight installation on said frame, is provided with compatible with first guide rail on first sliding block and second sliding block
Groove, first sliding block are connected with first slider connecting plate, and second sliding block and second slider connecting plate connect
It connects, first slider connecting plate is for installing the bar magnet frame, and second slider connecting plate is for installing the bar magnet set
Frame.
8. nucleic acid-extracting apparatus as claimed in claim 7, which is characterized in that the sliding equipment includes also the second guide rail, the
Three sliding blocks, Four-slider, third slider connecting plate and Four-slider connecting plate, second guide rail are vertically mounted on the frame
On frame, and first guide rail and second guide rail are installed respectively on two opposite sides of the frame, and the third is sliding
Groove compatible with second guide rail is provided on block and the Four-slider, the third sliding block and the third are sliding
The connection of block connecting plate, the Four-slider are connected with the Four-slider connecting plate, and the third slider connecting plate is for installing
The bar magnet frame, the Four-slider connecting plate is for installing the bar magnet stock.
9. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that the bar magnet frame includes bar magnet connecting plate and magnetic pole
Frame, there are three the magnetic pole framves for connection on the bar magnet connecting plate, and the magnetic pole frame is for installing bar magnet.
10. nucleic acid-extracting apparatus as described in claim 1, which is characterized in that the bar magnet stock includes bar magnet set connecting plate
Mounting rack is covered with bar magnet, bar magnet covers connection on connecting plate, and there are three the bar magnets to cover mounting rack, and the bar magnet set mounting rack is used for
Bar magnet set is installed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811425934.4A CN109266547A (en) | 2018-11-27 | 2018-11-27 | Nucleic acid-extracting apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811425934.4A CN109266547A (en) | 2018-11-27 | 2018-11-27 | Nucleic acid-extracting apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109266547A true CN109266547A (en) | 2019-01-25 |
Family
ID=65185906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811425934.4A Pending CN109266547A (en) | 2018-11-27 | 2018-11-27 | Nucleic acid-extracting apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109266547A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048429A (en) * | 2020-09-25 | 2020-12-08 | 杭州迪安生物技术有限公司 | High-efficient of nucleic acid extraction appearance is drawed and is shaken module and nucleic acid extraction appearance |
| CN113862255A (en) * | 2021-09-22 | 2021-12-31 | 南京优而生物科技发展有限公司 | Method for extracting and purifying nucleic acid by one-step method and purification device |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008102772A1 (en) * | 2007-02-22 | 2008-08-28 | Toyo Boseki Kabushiki Kaisha | Nucleic acid amplification system |
| CN103881893A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN103923831A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Instrument and method for extraction of bioactive substances |
| KR20160000001A (en) * | 2014-05-30 | 2016-01-04 | 주식회사 바이오포커스 | Apparatus for automatically performing analysis of immune |
| US20160245834A1 (en) * | 2013-07-16 | 2016-08-25 | Genmag Biotechnology Co., Ltd. | Apparatus and method for extracting biologically active substances through magnetic beads method |
| CN205874406U (en) * | 2016-07-28 | 2017-01-11 | 成都赛安普飞科技有限公司 | Cell intercepting device |
| CN205893243U (en) * | 2016-08-22 | 2017-01-18 | 杭州奥盛仪器有限公司 | Eccentric wobbling nucleic acid extraction equipment |
| CN106520547A (en) * | 2017-01-13 | 2017-03-22 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Nucleic acid extraction equipment |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108441423A (en) * | 2018-04-28 | 2018-08-24 | 绍兴迅敏康生物科技有限公司 | Instrument for extracting nucleic acid |
| CN209322905U (en) * | 2018-11-27 | 2019-08-30 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
-
2018
- 2018-11-27 CN CN201811425934.4A patent/CN109266547A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008102772A1 (en) * | 2007-02-22 | 2008-08-28 | Toyo Boseki Kabushiki Kaisha | Nucleic acid amplification system |
| CN103923831A (en) * | 2013-01-15 | 2014-07-16 | 北京金麦格生物技术有限公司 | Instrument and method for extraction of bioactive substances |
| US20160245834A1 (en) * | 2013-07-16 | 2016-08-25 | Genmag Biotechnology Co., Ltd. | Apparatus and method for extracting biologically active substances through magnetic beads method |
| CN103881893A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| KR20160000001A (en) * | 2014-05-30 | 2016-01-04 | 주식회사 바이오포커스 | Apparatus for automatically performing analysis of immune |
| CN205874406U (en) * | 2016-07-28 | 2017-01-11 | 成都赛安普飞科技有限公司 | Cell intercepting device |
| CN205893243U (en) * | 2016-08-22 | 2017-01-18 | 杭州奥盛仪器有限公司 | Eccentric wobbling nucleic acid extraction equipment |
| CN106520547A (en) * | 2017-01-13 | 2017-03-22 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Nucleic acid extraction equipment |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108441423A (en) * | 2018-04-28 | 2018-08-24 | 绍兴迅敏康生物科技有限公司 | Instrument for extracting nucleic acid |
| CN209322905U (en) * | 2018-11-27 | 2019-08-30 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112048429A (en) * | 2020-09-25 | 2020-12-08 | 杭州迪安生物技术有限公司 | High-efficient of nucleic acid extraction appearance is drawed and is shaken module and nucleic acid extraction appearance |
| CN113862255A (en) * | 2021-09-22 | 2021-12-31 | 南京优而生物科技发展有限公司 | Method for extracting and purifying nucleic acid by one-step method and purification device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109266547A (en) | Nucleic acid-extracting apparatus | |
| CN105259016B (en) | A kind of full-automatic digestion device | |
| CN204013087U (en) | Motor and there is its electric automobile | |
| CN209322905U (en) | Nucleic acid-extracting apparatus | |
| CN102366725A (en) | Automatic sampling gun | |
| CN205138874U (en) | Full -automatic digestion instrument of multi partition heating | |
| CN103992940A (en) | Instrument for extracting bioactive substance | |
| EP4332582A1 (en) | Reagent transfer device and immunological diagnostic apparatus | |
| CN107287194A (en) | Extraction element | |
| CN107034125A (en) | Extraction element | |
| CN107022469A (en) | Movable mechanism | |
| CN202182895U (en) | Single-beam four-channel automatic sampling gun | |
| CN105928766B (en) | Biochip point sample instrument and its print table | |
| CN217265654U (en) | Nucleic acid extraction device | |
| CN110042054A (en) | A kind of automation magnetic bead separating device | |
| CN202182890U (en) | Y-direction driving mechanism for automatic sample-adding guns | |
| CN202182891U (en) | Needle lifting transmission device of automatic sample-adding gun | |
| CN202182900U (en) | Automatic sample adding gun | |
| CN202182901U (en) | Space cam dissecting needle mechanism for automatic sampling gun | |
| CN202182899U (en) | Double-guiderail sliding device of automatic sampling gun | |
| CN205080149U (en) | Move Z axle construction of liquid module | |
| CN214088479U (en) | Novel nucleic acid extraction appearance | |
| CN208327983U (en) | Nucleic acid extraction liquid distributing device | |
| CN220367313U (en) | Mounting structure, sampling mechanism and blood cell analyzer | |
| CN205246685U (en) | Full -automatic four -channel application of sample rifle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190125 |