CN205874406U

CN205874406U - Cell intercepting device

Info

Publication number: CN205874406U
Application number: CN201620801640.7U
Authority: CN
Inventors: 黄文卉
Original assignee: Chengdu Tour Amp Technology Co Ltd Fly
Current assignee: Chengdu Tour Amp Technology Co Ltd Fly
Priority date: 2016-07-28
Filing date: 2016-07-28
Publication date: 2017-01-11
Anticipated expiration: 2026-07-28

Abstract

The utility model discloses a cell intercepting device, the device is become by drive arrangement, conversion equipment, two filter that contain hollow fiber filtration post and diaphragm pump that make power with linear electric motor, drive arrangement drive diaphragm pump makes the fluid that contains the cell pass through hollow fiber and filters post entering diaphragm pump, and this process also can be reverse, through a conversion equipment, drive arrangement can only drive wherein any one diaphragm pump operation or two diaphragm pumps move simultaneously, and the conversion can be manual or automatic, when one of them diaphragm pump operation, another filter can carry out operations such as consumptive material change, troubleshooting, sterilization, unnecessary interruption of production, and adjustable diaphragm diaphragm displacement, diaphragm pump functioning speed and acceleration, driving moment. This device easy operation, operate steadily, the pair cell does not have instantaneous shearing force and produces, can be in succession not interruption of production, degree of automation is high, production efficiency is high.

Description

A kind of cell retention device

Technical field

This utility model relates to a kind of cell retention device, belongs to field of pharmaceutical equipment.

Background technology

In recent years, the interest of continuous processing is being continuously increased.Compared with batch or fed-batch, the main distinction of perfusion Advantage is holdup time, higher cell density and the corresponding higher every day that purpose product is shorter in bioreactor Unit volume productivity, longer incubation time, more healthy cell state and cell growth environment preferably control.

In mammalian cell perfusion, cell separation equipment can be classified by different physical principles: filters, add Speed/gravity and, electric field.In large-scale culture, it is equipment operation that separation equipment is arranged on the main advantage outside reactor More convenient, particularly when equipment performance starts to occur declining, it is convenient to be replaced or sterilizing again.

In large-scale suspension cell culture, more use ATF, TFF, rotary filter or tilt sedimentation etc..Base In the Perfusion of doughnut, it it is the method uniquely supporting pole high-cell-density cultivation.Fouling membrane during application ATF, TFF And the purpose product section caused retains and also happens occasionally.Existing ATF equipment and TFF equipment cannot realize in unbroken situation Under carry out hollow fiber column replacing, and existing ATF equipment relies on compressed air and vacuum source to be that power drive diaphragm pump runs, When diaphragm pump runs commutation, power is switched to the vacuum of negative pressure by the compressed air of malleation；Diaphragm pump produces when running commutation Significant shock, and give diaphragm sheet and cultivate the cell bigger instantaneous shearing force of generation, it reduce the service life of diaphragm sheet, also increase Add the possibility that cell is destroyed because of excessive shearing force.

Utility model content

This utility model relates to a kind of filtration system, more precisely, this utility model relates to a kind of spy for fluid It it not the filtration system of biofluid.This filtration system includes two filters, and one end of filter is connected to bioreactor, The other end is connected with driving means.A kind of form of the present utility model is that two filters divide row both sides, during driving means is positioned at Between；Driving means is moved, and can order about two simultaneously or only order about wherein any one filter work；Make fluid anti-from biology Answering and flow in device and by two or wherein any one filter, fluid is checker stream with the reciprocating motion of driving means To.Driving means can with set acceleration or time acceleration or deceleration to setting the speed of service, the power that can also set Square exports.

Above-mentioned filter includes a hollow fibre filtering post, a membrane pump, and is connected to biological anti-for adaptation Answer the joint needed for device and driving means, adapter, for ease of the instrument needed for observation, detecting system running status, instrument.In One end (that is arrival end of hollow fibre filtering post, hereinafter referred to as arrival end) of hollow fiber Filter column is by pipeline connecting parts even Receiving bioreactor, the other end (port of export of hollow fibre filtering post, the hereinafter referred to as port of export) connects membrane pump；Membrane pump Diaphragm sheet be connected to driving means.

This utility model has some advantages not available for other system, including obtaining constant and low cutting Shear force exports, and this is more beneficial for such as the processing of the fragile material such as zooblast and biomolecule.Other benefits also body of this system Present automatization of the present utility model and efficientibility；I.e. two filters can run simultaneously also can individually be transported by arbitrary filter OK, it is advantageous in that: 1) runs simultaneously and will significantly improve production efficiency；2) one of them filter can be run during a small amount of production, With the cost that economizes on resources；3) operation of this filter can be automatically stopped during the fault such as one of them filter generation blocking, with Change consumptive material and/or fix a breakdown, and not affecting the operation of another filter, i.e. producing and do not interrupt.Other benefits also include can Accurately to control the diaphragm sheet speed of service and velocity variations, diaphragm pump commutation steadily, can extend the life-span of diaphragm sheet.

Accompanying drawing explanation

Fig. 1 is that cell retention device faces structural representation

Fig. 2 is filtration system structural representation

Fig. 3 is driving device structure schematic diagram

Conversion device structure schematic diagram when Fig. 4 is bilateral operation

Conversion device structure schematic diagram when Fig. 5 is to run on the left of single

Conversion device structure schematic diagram when Fig. 6 is single race running

Detailed description of the invention

Illustrate detailed description of the invention of the present utility model, protection domain of the present utility model not office below in conjunction with the accompanying drawings It is limited to the following stated.

Fig. 1 is to have a liquid storage container (bioreactor) according to what this utility model represented, has two filters, These two filters run simultaneously, and fluid outlet is at the example of the filtration system on hollow fibre filtering post top.

This filtration system comprises two filter A and B, filter A and filter B and has the effect of the same convection cell filtration With；For reaching this effectiveness, two set filter A and B can have identical or different structure and composition part, is also not excluded for its group One-tenth part has difference in size, specification.For ease of narration, what Fig. 1 represented is one of which situation, i.e. filter A and B has There is identical structure and composition part.

Pipeline connecting parts 2 connects a bioreactor device 1 and hollow fibre filtering post 3 and hollow fibre filtering post 4.

One end of pipeline connecting parts 2 is connected with bioreactor 1 by least one valve 35, is wrapped by one at the other end Containing but be not limited to the device 40 of threeway and be divided into two ports, be connected and hollow with the arrival end 38 of hollow fibre filtering post 3 respectively The arrival end 39 of fiber filter post 4 connects.But pipeline connecting parts 2 is not limited to above form, also can be from bioreactor 1 Two outlets be respectively connecting to the arrival end of two hollow fibre filtering posts 3,4, and without the device by being similar to threeway. Sterile medium can pass through valve 36, valve 37 enters into and through pipeline connecting parts 2 and flow to the arrival end of hollow fibre filtering post 3,4.

Due to state herein be filter A and filter B has identical structure and ingredient, for simplifying narration, Only filter A is done accompanying drawing explanation herein.That represent such as Fig. 2 is filter A.

The port of export 41 of hollow fibre filtering post 3 connects membrane pump 7, and membrane pump 7 is divided into upper compartment 17 by diaphragm 18 With lower compartment 19, fluid can from the port of export of doughnut Filter column 3 flows into compartment 17, also can flow into hollow from upper compartment 17 The port of export of fiber filter post 3；Offer porose 24 on the wall of lower compartment 19, under air or other medium inlet and outlet every Room, a kind of device including but not limited to valve can be connected on hole 24.

One end of push rod 11 is entered lower compartment 17 through membrane pump housing 43 and is connected with diaphragm sheet 18 by a kind of mode； This mode can by be any be suitable for the two connection in the way of, include but not limited to embed, tight-holding type connect, threaded, true Suction is attached.The other end of push rod 11 by a kind of applicable by the way of be connected with push pedal 15, this applicable mode can be to divide From connection and inseparable connection such as welding, being also not excluded for push rod 11 with push pedal 15 is an one-piece parts.

What Fig. 3 represented is driving means.Linear electric motors 9 comprise stator 32 and mover 31；Stator 32 is arranged on support means Maintain static on 45；Mover 31 can do reciprocating linear motion according to the speed set, acceleration, time, stroke, mover 31 One end is connected with driving plate 14, also drives driving plate 14 to be synchronized with the movement when mover 31 moves.

A kind of conversion equipment 48 is arranged on driving plate 14, and this conversion equipment 48 comprises stator 10 and mover 13；Mover 13 Can do reciprocating linear motion, and can stop in the position set；Described in the utility model require mover 13 at least Fig. 4, figure 5, three shown in Fig. 6 operating position.

As shown in Figure 4, now the mover 13 of conversion equipment 48 is centrally located, and by push pedal 15, push pedal 16 and driving Plate 14 connects, then the mover 31 of linear electric motors 9 can drive push pedal 15 to move with push pedal 16 when moving simultaneously, and filter A, B are simultaneously Work.

As it is shown in figure 5, now the mover 13 of conversion equipment 48 is positioned at leftward position, and push pedal 15 is connected with driving plate 14 Connect, and push pedal 16 departed from driving plate 14, then the mover 31 of linear electric motors 9 can only drive push pedal 15 to be synchronized with the movement when moving, Filter A works.

As shown in Figure 6, now the mover 13 of conversion equipment 48 is positioned at right positions, and push pedal 16 is connected with driving plate 14 Connect, and push pedal 15 departed from driving plate 14, then the mover 31 of linear electric motors 9 can only drive push pedal 16 to be synchronized with the movement when moving, Filter B works.

Filter mentioned above can simplify, as can be directly driven diaphragm sheet 18,21 by linear electric motors 9, or directly Driving can promote the device of diaphragm sheet 18,21, and without using conversion equipment.

Linear electric motors 9 mentioned above and conversion equipment 48 can be any devices that can produce linear motion, bag Include but be not limited to all kinds of linear electric motors, screw mechanism, slider-crank mechanism, pulley mechanism, cam mechanism, tape handler, rope Drive mechanism, pinion and rack, cylinder, hydraulic cylinder, electric cylinder, electric pushrod.

Claims

1. a cell retention device:

A) at least one linear motion device, the motion parts of this linear motion device can set movement travel in, with The speed or time, setting torsion, acceleration or the acceleration time that set do reciprocating linear motion, and can be in any position in stroke Put stopping；

B) at least one conversion equipment, when filter occur blocking in the case of, linear motion device fixing torsion and time Under between, movement travel can change, and can pass through the automatic converting filter of conversion equipment after reaching the change number of times set, and Pointed out by controller display screen or report to the police；

C) at least one conversion equipment, conversion equipment can make the membrane pump connection of the linear motion device described in a and filtration system Dynamic and disengaging links；Furtherly, conversion equipment can make linear motion device and the linkage of two membrane pumps or only with wherein appoint A diaphragm pump of anticipating links.

In a kind of cell retention device the most according to claim 1, at least one air scoop is connected with membrane pump, and gas can From this air scoop inlet and outlet membrane pump.

In a kind of cell retention device the most according to claim 1, at least one exports (or entrance) to 6 sterile mediums It is connected with membrane pump.

In a kind of cell retention device the most according to claim 1, the motion parts of linear motion device is filled by conversion Put and be directly connected to the diaphragm sheet in membrane pump.

In a kind of cell retention device the most according to claim 1, the motion parts of linear motion device is filled by conversion The part put and be connected diaphragm sheet connects.

In a kind of cell retention device the most according to claim 1, the diaphragm of a special gradient thickness, have one The individual butt joint can being connected with drive connection bar.

In a kind of cell retention device the most according to claim 1, the filter being connected with membrane pump can use outside carrying Shell or Stainless Steel Shell.