CN107287194A - Extraction element - Google Patents
Extraction element Download PDFInfo
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- CN107287194A CN107287194A CN201710636784.0A CN201710636784A CN107287194A CN 107287194 A CN107287194 A CN 107287194A CN 201710636784 A CN201710636784 A CN 201710636784A CN 107287194 A CN107287194 A CN 107287194A
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- magnetosheath
- frame
- connector
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- extraction element
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
Include pedestal the invention discloses a kind of extraction element（1）, heater（5）, kit（14）, filter core（3‑2）, fan（3‑1）, movable mechanism（8）, shell（2）, magnetosheath（33）, magnetosheath frame（7）, bar magnet（140）With bar magnet frame（6）.During being extracted using the extraction element to nucleic acid, accommodating cavity（32）Side wall be provided with the first air admission hole（31a or 31b）, air-flow passes through the first air admission hole（31a or 31b）, accommodating cavity（32）, filter core（3‑2）, fan（3‑1）Realize circulation；Movable mechanism（8）Magnetosheath frame can be driven respectively（7）, bar magnet frame（6）Carry out the movement of horizontal direction or vertical direction so that bar magnet（140）With magnetosheath（33）Between can coordinate automatically.It can avoid the cross pollution between sample during nucleic acid extraction.
Description
Technical field
The present invention relates to reaction unit technical field, more particularly to a kind of extraction element.
Background technology
Nucleic acid is the main study subject of genetic engineering and protein engineering research field, the quantitative inspection of nucleic acid molecules
Survey and study for legal medical expert's detection, gene sequencing, PCR amplifications, gene knockout, gene cloning, transgenosis, paternity test and
The application of gene sequencing is of great significance.
It is the main stream approach of current nucleic acid extraction based on paramagnetic particle method nucleic acid extraction.It the advantage is that the nucleic acid purity of extraction
High, concentration is big, and extraction operating process is simple, the used time is short, safety non-toxic, easily realizes that automation, high pass quantify.
Nucleic acid extraction instrument uses conventional automated technique in the market, and future realizes pollution-free, the height for extracting nucleic acid
Quality, intelligent network it is information-based by be future development trend.
The content of the invention
In view of this, the invention provides a kind of extraction element, its can avoid during nucleic acid extraction between sample compared with
Difference pollution, thus more suitable for practicality.
In order to reach above-mentioned first purpose, the technical scheme for the extraction element that the present invention is provided is as follows：
The extraction element that the present invention is provided includes pedestal（1）, heater（5）, kit（14）, filter core（3-2）, fan（3-
1）, movable mechanism（8）, shell（2）, magnetosheath（33）, magnetosheath frame（7）, bar magnet（140）With bar magnet frame（6）,
The heater（5）It is fixedly connected on the pedestal（1）,
The kit（14）It is arranged at the heater（5）On,
The movable mechanism（8）One end be fixedly connected on the pedestal（1）, the magnetosheath frame（7）It is fixedly connected on described
Movable mechanism（8）The other end, the movable mechanism（8）For controlling the bar magnet frame（6）With magnetosheath frame（7）Exist respectively
The movement of horizontal direction and vertical direction,
The shell（2）It is located at the kit（14）, magnetosheath（33）, magnetosheath frame（7）, movable mechanism（8）, filter core（3-
2）And fan（3-1）Outside, and in the shell（2）With the pedestal（1）Between form accommodating cavity（32）,
The filter core（3-2）It is fixedly connected on the accommodating cavity（32）Outer wall on, also, the filter core（3-2）It is completely covered
State accommodating cavity in residence（32）,
The fan（3-1）It is arranged on the filter core（3-2）Top,
The accommodating cavity（32）Side wall be provided with the first air admission hole（31a or 31b）, air-flow passes through the first described air admission hole
（31a or 31b）, accommodating cavity（32）, filter core（3-2）, fan（3-1）Realize circulation,
The magnetosheath frame（7）It is provided with the first perforating, the magnetosheath（33）Institute is arranged in by the first described perforating
State magnetosheath frame（7）On,
The bar magnet frame（6）Provided with second of perforating, the bar magnet（140）Institute is arranged in by second of perforating
State bar magnet frame（6）On,
The bar magnet（140）With the magnetosheath（33）Position in space is corresponding so that the bar magnet（140）It can extend into
The magnetosheath（33）In.
The extraction element that the present invention is provided can be also applied to the following technical measures to achieve further.
Preferably, the extraction element also includes foot pad（16）, the foot pad（16）It is fixedly connected on the pedestal（1）
Bottom, for the foot pad to be made（16）Material be selected from rubber.
Preferably, the heater（5）Including hot well（5-1）, thermal source, the kit（14）It is removably disposed
In the hot well（5-1）Top, the thermal source is used for the hot well（5-1）Heat energy is provided.
Preferably, the extraction element also includes at least one pair of elastomer（5-3）, the heater（5）Top
Provided with heating panel（5-2）, at least one pair of described elastomer（5-3）It is arranged at the heating panel（5-2）Two ends, when described
Kit（14）It is placed in the heating panel（5-2）On when, the elastomer（5-3）Holding at the two ends of the kit,
Also, the elastomer（5-3）In compressive state.
Preferably, the extraction element also includes magnetosheath frame（7-1）, magnetosheath（33）, pre- pressure ball（7-4）,
The magnetosheath frame（7-1）It is fixedly connected on the magnetosheath frame（7）,
The magnetosheath（33）Wear and penetrate in the magnetosheath frame（7-1）,
The magnetosheath frame（7-2）It is provided with storage tank, the magnetosheath（33）Radial dimension ＞ described in storage tank radial dimension,
So that, when the magnetosheath（33）With the magnetosheath frame（7-1）During cooperation, the magnetosheath（33）With the magnetosheath frame（7-1）Between
It is interference fit,
The pre- pressure ball（7-4）It is arranged at the storage tank and magnetosheath（33）Between gap in.
Preferably, the extraction element also includes shell（2）,
The shell（2）The pedestal is fixedly connected on by its base edge（1）,
The shell（2）Side wall provided with second air admission hole（35a or 35b）, second of air admission hole（35a or 35b）
With the first described air admission hole（31a or 31b）Position it is corresponding,
The shell（2）Side wall on be additionally provided with venthole（34a or 34b）, the venthole（34a or 34b）So that described
Fan（3-1）The gas outflow pumped out.
Preferably, the movable mechanism includes moving horizontally component（A）With vertical shift component（B）；
It is described to move horizontally component（A）Including stepper motor（107）, the first connecting plate（102）, the first limiting plate（131）, first
Sliding block（106）, the second limiting plate（132）, screw rod（105）With the first feed rod（103）,
First connecting plate（102）, the first limiting plate（131）With the second limiting plate（132）It is vertically connected at the pedestal
（1）,
The stepper motor（107）It is fixedly connected on first connecting plate（102）, the stepper motor（107）Output shaft
It is fixedly connected on the screw rod（105）One end, the screw rod（105）The other end and first sliding block（106）Between structure
Into screw thread pair, first sliding block（106）Along first feed rod（103）Axially at least provided with first through hole（134）, institute
State the first feed rod（103）With first sliding block（106）Between pass through first through hole（134）Constitute sliding pair so that described the
One sliding block（106）Can be in first limiting plate（131）With the second limiting plate（132）Between slide；
The vertical shift component（B）It is fixedly connected on first sliding block（106）；
The bar magnet frame（6）With magnetosheath frame（7）It is connected to the vertical shift component（B）So that the bar magnet frame（6）With
Magnetosheath frame（7）Separately in the vertical direction is moved.
Preferably, the vertical shift component B includes the second connecting plate（108）, the first electric rotating machine（109）, second
Electric rotating machine（133）, the first tape handler（110）, the second tape handler（118）, clutch shaft bearing（112）, second bearing
（114）, 3rd bearing（119）, fourth bearing（123）, slide rail（125）, the second sliding block（126）, the 3rd sliding block（127）, first
Connector（116）With the second connector（122）,
The clutch shaft bearing（112）, second bearing（114）, 3rd bearing（119）, fourth bearing（123）It is embedded at respectively described
Second connecting plate（108）, wherein, the clutch shaft bearing（112）And second bearing（114）It is described in same vertical straight line
3rd bearing（119）And fourth bearing（123）In same vertical straight line；
First electric rotating machine（109）Output shaft pass through the clutch shaft bearing（112）The first band is fixedly connected on to pass
Motivation structure（110）The first driving wheel（111）；Second electric rotating machine（133）Output shaft pass through second V belt translation
Mechanism（118）The second driving wheel（139）；
The slide rail（125）Vertically it is fixedly connected on second connecting plate（108）On, second sliding block（126）、
3rd sliding block（127）Respectively with the slide rail（125）Constitute sliding pair；
First connector（116）One end be fixedly connected on second sliding block（126）, first connector（116）
The other end be fixedly connected on first tape handler（110）The first conveyer belt（117）；
Second connector（122）One end be fixedly connected on the 3rd sliding block（127）, second connector（122）
The other end be fixedly connected on second tape handler（118）The second conveyer belt（136）；
First connector（116）Pass through its external part（128）It is fixedly connected on the bar magnet frame（6）；
Second connector（122）Pass through its external part（129）It is fixedly connected on the magnetosheath frame.
Preferably,
First conveyer belt（117）It is provided with the first engaging tooth（137）, first connector（116）The other end be provided with
Second engaging tooth, first connector（116）Pass through first engaging tooth（137）, the engagement between the second engaging tooth consolidates
Fixed connection；
Second conveyer belt（136）It is provided with the 3rd engaging tooth（138）, second connector（122）The other end be provided with
4th engaging tooth, second connector（122）Pass through the 3rd engaging tooth（138）, the engagement between the 4th engaging tooth consolidates
Fixed connection.
Preferably, the extraction element also includes the 3rd connecting plate（101）, the 3rd connecting plate（101）By it
One face and the pedestal（1）It is fixedly connected, first connecting plate（102）, the first limiting plate（131）With the second limiting plate
（132）It is vertically connected at the 3rd connecting plate（101）Another face.
Preferably, the extraction element also includes the second feed rod（104）, second feed rod（104）With described first
Feed rod（103）It is parallel,
First sliding block（106）Along second feed rod（104）Axial direction be additionally provided with the second through hole（135）, second light
Thick stick（104）With first sliding block（106）Between pass through second through hole（135）Constitute sliding pair.
Preferably, the extraction element also includes uviol lamp（19）, the uviol lamp（19）It is by sending ultraviolet light
The accommodating cavity（32）Gnotobasis is provided.
Preferably, the extraction element also includes power outlet（130）, the electricity of the consuming parts in the extraction element
The power connection end of source line is respectively arranged at the power outlet（130）It is interior.
Preferably, the extraction element also includes the first locating part（141）,
In second connecting plate（108）On, along first tape handler（110）The first conveyer belt（117）Motion
Direction is provided with the first elongate slot（144）So that it is arranged at first connector（116）The plush copper of the other end（142）It is placed in
First elongate slot（144）It is interior；
First locating part（141）Across first elongate slot（144）Set, when first connector（116）Upwards
When moving to extreme position, first connector is arranged at（116）The plush copper of the other end（142）By first locating part
（141）Backstop.
Preferably, the extraction element also includes the second locating part（143）,
In second connecting plate（108）On, along second tape handler（118）The second conveyer belt（136）Motion
Direction is provided with the second elongate slot so that be arranged at second connector（122）It is narrow that the plush copper of the other end is placed in described second
In elongated slot；
Second locating part（143）Set across second elongate slot, when second connector（122）Move upward to
During extreme position, second connector is arranged at（122）The plush copper of the other end is by second locating part（143）Backstop.
During the extraction element provided using the present invention is extracted to nucleic acid, accommodating cavity（32）Side wall be provided with
The first air admission hole（31a or 31b）, air-flow passes through the first air admission hole（31a or 31b）, accommodating cavity（32）, filter core（3-2）, wind
Fan（3-1）Realize circulation；Movable mechanism（8）Magnetosheath frame can be driven respectively（7）, bar magnet frame（6）Carry out horizontal direction
Or the movement of vertical direction so that bar magnet（140）With magnetosheath（33）Between can coordinate automatically.It can avoid nucleic acid extraction
During cross pollution between sample.
Brief description of the drawings
By reading the detailed description of hereafter preferred embodiment, various other advantages and benefit is common for this area
Technical staff will be clear understanding.Accompanying drawing is only used for showing the purpose of preferred embodiment, and is not considered as to the present invention
Limitation.And in whole accompanying drawing, identical part is denoted by the same reference numerals.In the accompanying drawings：
The basic structure schematic diagram for the extraction element that Fig. 1 provides for the present invention；
The operation principle schematic diagram for the extraction element that Fig. 2 provides for the present invention；
The structural representation for the heater applied in the extraction element that Fig. 3 present invention are provided；
Fig. 4 is Fig. 3 typical cross section structure diagram；
The structural representation of one typical directions of the magnetosheath frame applied in the extraction element that Fig. 5 provides for the present invention；
The structural representation of another typical directions of the magnetosheath frame applied in the extraction element that Fig. 6 provides for the present invention；
The matching relationship structural representation that the bar magnet applied in the extraction element that Fig. 7 provides for the present invention is matched somebody with somebody with magnetosheath frame；
Matching relationship between the movable mechanism and magnetosheath frame, bar magnet frame applied in the extraction element that Fig. 8 provides for the present invention
Structural representation in an angle；
Matching relationship between the movable mechanism and magnetosheath frame, bar magnet frame applied in the extraction element that Fig. 9 provides for the present invention
Structural representation in another angle；
The structural representation for the movable mechanism applied in the extraction element that Figure 10 provides for the present invention；
Structural representation after one block of side plate that Figure 11 dismantles on shell for the extraction element that the present invention is provided；
The a chain of schematic diagram for the upper suction paramagnetic particle method nucleic acid extraction principle that Figure 12 is related to for the extraction element that the present invention is provided.
Embodiment
The present invention is solves the problem of prior art is present there is provided a kind of extraction element, and it can avoid nucleic acid extraction mistake
Poor pollution in journey between sample, thus more suitable for practicality.
Further to illustrate the present invention to reach the technological means and effect that predetermined goal of the invention is taken, below in conjunction with
Accompanying drawing and preferred embodiment, to according to extraction element proposed by the present invention, its embodiment, structure, feature and its effect,
Describe in detail as after.In the following description, what different " embodiment " or " embodiment " referred to is not necessarily same embodiment.This
Outside, special characteristic, structure or the feature in one or more embodiments can be combined by any suitable form.
The terms "and/or", only a kind of incidence relation for describing affiliated partner, represents there may be three kinds of passes
System, for example, A and/or B, is specifically interpreted as：A and B can be included simultaneously, can be with individualism A, can also individualism
B, can possess above-mentioned three kinds of any situations.
Referring to accompanying drawing 1~11, the extraction element that the present invention is provided includes pedestal 1, heater 5, kit 14, filter core 3-
2nd, fan 3-1, movable mechanism 8, shell 2, magnetosheath 33, magnetosheath frame 7, bar magnet 140 and bar magnet frame 6.Heater 5 is fixedly connected
In pedestal 1.Kit 14 is arranged on heater 5.One end of movable mechanism 8 is fixedly connected on pedestal 1, and magnetosheath frame 7 is consolidated
Surely the other end of movable mechanism 8 is connected to, movable mechanism 8 is used to control bar magnet frame 6 and the difference of magnetosheath frame 7 in the horizontal direction
With the movement of vertical direction.Shell 2 is located at kit 14, magnetosheath 33, magnetosheath frame 7, movable mechanism 8, filter core 3-2 and fan
3-1 outside, and the formation accommodating cavity 32 between shell 2 and pedestal 1.Filter core 3-2 is fixedly connected on the outer wall of accommodating cavity 32,
Also, filter core 3-2 covers all accommodating cavity 32.Fan 3-1 is arranged on filter core 3-2 top.Set on the side wall of accommodating cavity 32
There is the first air admission hole 31a or 31b, air-flow passes through the first air admission hole 31a or 31b, accommodating cavity 32, filter core 3-2, fan 3-1
Realize circulation.Magnetosheath frame 7 is provided with the first perforating, and magnetosheath 33 is arranged on magnetosheath frame 7 by the first perforating.
Bar magnet frame 6 is arranged on bar magnet frame 6 provided with second of perforating, bar magnet 140 by second of perforating.Bar magnet 140 and magnetic
Set 33 is corresponding in the position in space so that bar magnet 140 can extend into magnetosheath 33.
During being extracted using the extraction element that provides of the present invention to nucleic acid, the side wall of accommodating cavity 32 is provided with the
A kind of air admission hole 31a or 31b, air-flow is realized by the first air admission hole 31a or 31b, accommodating cavity 32, filter core 3-2, fan 3-1
Circulation；Movable mechanism 8 can drive the shifting of magnetosheath frame 7, the progress horizontal direction of bar magnet frame 6 or vertical direction respectively
It is dynamic so that can to coordinate automatically between bar magnet 140 and magnetosheath 33.It can avoid the intersection during nucleic acid extraction between sample dirty
Dye.
Wherein, extraction element also includes foot pad 16, and foot pad 16 is fixedly connected on the bottom of pedestal 1, for foot pad 16 to be made
Material be selected from rubber.The coefficient of friction of rubber in itself is larger, and has deformation space when being pressurized, therefore, it is possible to avoid carrying
Take device in application process, slide on the table, also, when slight be pressurized occurs for extraction element, it has micro-
The effect of buffering, the damage that extraction element can be avoided to be subjected to when direct rigidity is pressurized.
Wherein, as a kind of concrete implementation mode of heater 5, heater 5 includes hot well 5-1, thermal source, reagent
Box 14 is removable installed in hot well 5-1 top, and thermal source is used to provide heat energy to hot well 5-1.In this case, thermal source is first
Hot well 5-1 is first heated, the heat of heating kit 14 passes to the reagent in kit 14 by hot well 5-1, due to hot well 5-1
It is scattered more uniform in the bottom of kit 14, thereby it is ensured that the multi-hole position thermally equivalent of kit 14.
Wherein, extraction element also includes at least one pair of elastomer 5-3（Including 5-3a and 5-3b）, the top of heater 5
Provided with heating panel 5-2, at least one pair of elastomer 5-3（Including 5-3a and 5-3b）Heating panel 5-2 two ends are arranged at, work as examination
When agent box 14 is placed on heating panel 5-2, a pair of elastomer 5-3（Including 5-3a and 5-3b）Holding at the two ends of kit,
Also, a pair of elastomer 5-3（Including 5-3a and 5-3b）In compressive state.In this case, kit 14 passes through a pair
Elastomer 5-3（Including 5-3a and 5-3b）Holding is on heating panel 5-2, due to now a pair of elastomer 5-3（Including 5-3a and
5-3b）In compressive state, active force is accommodated therefore, it is possible to provide kit 14 so that kit 14 can preferably be consolidated
It is scheduled on heating panel 5-2.In addition, when the cross-sectional area of kit 14 is less than heating panel 5-2, can also be in heating panel
Dividing plate 5-12 is set on 5-2, subregion is carried out to heating panel 5-2, so as on same heating panel 5-2, can house more
Kit 14.
Wherein, extraction element also includes magnetosheath frame 7-1, magnetosheath 33, pre- pressure ball 7-4.Magnetosheath frame 7-1 is fixedly connected on magnetosheath
Frame 7.Magnetosheath 33, which is worn, to be penetrated in magnetosheath frame 7-1.Magnetosheath frame 7-2 is provided with storage tank, the footpath of the radial dimension ＞ storage tanks of magnetosheath 33
To size so that, it is interference fit between magnetosheath 33 and magnetosheath frame 7-1 when magnetosheath 33 and magnetosheath frame 7-1 cooperations.Pre- pressure ball
7-4 is arranged in the gap between storage tank and magnetosheath 33.In this case, can be more between magnetosheath 33 and magnetosheath frame 7-1
Fixed well, also, be easy to picking and placeing for magnetosheath 33.Wherein, each row magnetosheath 33 is worn to magnetosheath by a connecting piece 7-2
In the perforating set on frame 7-1, in order to be picked and placeed to each row magnetosheath 33.
Wherein, extraction element also includes shell 2.Shell 2 is fixedly connected on pedestal 1, the side wall of shell 2 by its base edge
Provided with second air admission hole 35a or 35b, second of air admission hole 35a or 35b and the first air admission hole 31a or 31b position phase
Venthole 34a or 34b are additionally provided with correspondence, the side wall of shell 2, venthole 34a or 34b cause the gas pumped out by fan 3-1
Outflow.In this case, the airflow direction in extraction element can be controlled so that reaction can be controlled better.
Wherein, movable mechanism includes moving horizontally component A and vertical shift component B.Moving horizontally component A includes stepping
Motor 107, the first connecting plate 102, the first limiting plate 131, the first sliding block 106, the second limiting plate 132, the light of screw rod 105 and first
Thick stick 103.First connecting plate 102, the first limiting plate 131 and the second limiting plate 132 are vertically connected at pedestal 1.Stepper motor 107
The first connecting plate 102 is fixedly connected on, the output shaft of stepper motor 107 is fixedly connected on one end of screw rod 105, screw rod 105
Constitute screw thread pair between the other end and the first sliding block 106, the first sliding block 106 is along the first feed rod 103 axially at least provided with the
One through hole 134, constitutes sliding pair so that the first sliding block between the first feed rod 103 and the first sliding block 106 by first through hole 134
106 can slide between the first limiting plate 131 and the second limiting plate 132.Vertical shift component B is fixedly connected on the first sliding block
106；Bar magnet frame 6 and magnetosheath frame 7 are connected to vertical shift component B so that bar magnet frame 6 and magnetosheath frame 7 separately exist
Moved on vertical direction.In this case, due to vertical shift component B be fixedly connected on move horizontally component A first slide
On block 106, when the first sliding block 106 is in the horizontal direction along during the axial movement of the first feed rod 103, vertical shift component B also with
The first sliding block 106 in the horizontal direction along the axial movement of the first feed rod 103.
Wherein, vertical shift component B include the second connecting plate 108, the first electric rotating machine 109, the second electric rotating machine 133,
First tape handler 110, the second tape handler 118, clutch shaft bearing 112, second bearing 114,3rd bearing the 119, the 4th
Bearing 123, slide rail 125, the second sliding block 126, the 3rd sliding block 127, the first connector 116 and the second connector 122.Clutch shaft bearing
112nd, second bearing 114,3rd bearing 119, fourth bearing 123 are embedded at the second connecting plate 108 respectively, wherein, clutch shaft bearing
112 and second bearing 114 be in same vertical straight line, 3rd bearing 119 and fourth bearing 123 are in same vertical straight line
On.The output shaft of first electric rotating machine 109 is fixedly connected on the first master of the first tape handler 110 by clutch shaft bearing 112
Driving wheel 111；The second driving wheel 139 that the output shaft of second electric rotating machine 133 passes through the second tape handler 118.The edge of slide rail 125
Vertical direction is fixedly connected on the second connecting plate 108, and the second sliding block 126, the 3rd sliding block 127 constitute with slide rail 125 slide respectively
Dynamic pair.One end of first connector 116 is fixedly connected on the second sliding block 126, and the other end of the first connector 116 is fixedly connected on
First conveyer belt 117 of the first tape handler 110.One end of second connector 122 is fixedly connected on the 3rd sliding block 127, the
The other end of two connectors 122 is fixedly connected on the second conveyer belt 136 of the second tape handler 118.First connector 116 leads to
Cross its external part 128 and be fixedly connected on bar magnet frame 6.Second connector 122 is fixedly connected on magnetosheath frame by its external part 129.
In this case, magnetosheath frame 7 can be moved up and down with the second conveyer belt 136 of the second tape handler 118, bar magnet frame 6
It can be moved up and down with the first conveyer belt 117 of the first tape handler 110, and first the 110, second band of tape handler is passed
Motivation structure 118 is individually controlled, therefore, and vertical shift component B can drive magnetosheath frame 7, the in the vertical direction of bar magnet frame 6 respectively
It is mobile.
Wherein, the first conveyer belt 117 is provided with the first engaging tooth 137, and the other end of the first connector 116 is nibbled provided with second
Tooth is closed, the first connector 116 is fixedly connected by the engagement between the first engaging tooth 137, the second engaging tooth.Second conveyer belt
136 are provided with the 3rd engaging tooth 138, and the other end of the second connector 122 is provided with the 4th engaging tooth, and the second connector 122 passes through
Engagement between 3rd engaging tooth 138, the 4th engaging tooth is fixedly connected.In this case, the first connector 116 and first is passed
The connection of band 117 and the connection of the second connector 122 and the second conveyer belt 136 is sent more to stablize.
Wherein, extraction element also includes the 3rd connecting plate 101, and the 3rd connecting plate 101 is fixed by one face with pedestal 1
Connection, the first connecting plate 102, the first limiting plate 131 and the second limiting plate 132 are vertically connected at the another of the 3rd connecting plate 101
One face.In this case, enable to movable mechanism 8 itself to constitute a single module and be easy to the integrated of product.
Wherein, extraction element also includes the second feed rod 104, and the second feed rod 104 is parallel with the first feed rod 103.First sliding block
106 are additionally provided with the second through hole 135 along the axial direction of the second feed rod 104, and second is passed through between the second feed rod 104 and the first sliding block 106
Through hole 135 constitutes sliding pair.In this case, moving horizontally simultaneously by the first feed rod 103, the due to the first sliding block 106
Two feed rods 104 are oriented to, therefore, and the movement of the first sliding block 106 in the horizontal direction is more stablized.
Wherein, extraction element also includes uviol lamp 19, and uviol lamp 19 is that accommodating cavity 32 provides sterile by sending ultraviolet light
Environment.
Wherein, extraction element also includes the power connection end point of the power line of the consuming parts in power outlet 130, extraction element
It is not arranged in power outlet 130.In this case, can be only by a power outlet 130 to being related in extraction element
All consuming parts be controlled.
Wherein, extraction element also includes the first locating part 141.On the second connecting plate 108, along the first tape handler
The direction of motion of 110 the first conveyer belt 117 is provided with the first elongate slot 144 so that be arranged at the other end of the first connector 116
Plush copper 142 is placed in the first elongate slot 144；First locating part 141 is set across the first elongate slot 144, when the first connector
116 when moving upward to extreme position, and the plush copper 142 for being arranged at the other end of the first connector 116 is stopped by the first locating part 141
Gear.In this case, the overrun of bar magnet frame 6 can be avoided.
Wherein, extraction element also includes the second locating part 143.On the second connecting plate 108, along the second tape handler
The direction of motion of 118 the second conveyer belt 136 is provided with the second elongate slot so that be arranged at the convex of the other end of the second connector 122
Head is placed in the second elongate slot；Second locating part 143 is set across the second elongate slot, when the second motion upwards of connector 122
During to extreme position, the plush copper of the other end of the second connector 122 is arranged at by the backstop of the second locating part 143.In this case,
The overrun of magnetosheath frame 7 can be avoided.
Referring to accompanying drawing 12, the principle that the extraction element provided using the present invention inhale paramagnetic particle method nucleic acid extraction is as follows：
1. magnetosheath moves up and down, lysed sample in hole position 1；
2. bar magnet magnetosheath is transferred to hole position 2；
3. magnetosheath is moved up and down, and magnetic bead is mixed in hole position 2；
4. hole position 1 is transferred back to after bar magnet magnetosheath absorption magnetic bead；
5. magnetosheath moves up and down, and adjusts with being combined in magnetic bead hole position 1；
6. the absorption of bar magnet magnetosheath combines the magnetic bead of nucleic acid；
7. bar magnet magnetosheath is transferred to hole position 3；
8. magnetosheath moves up and down, and washing combines the magnetic bead of nucleic acid for the first time in hole position 3；
9. the absorption of bar magnet magnetosheath combines the magnetic bead of nucleic acid, is transferred to hole position 4；
10. magnetosheath moves up and down, and second of washing combines the magnetic bead of nucleic acid in hole position 4；
(11) the absorption of bar magnet magnetosheath combines the magnetic bead of nucleic acid, is transferred to hole position 5；
(12) magnetosheath moves up and down, and third time washing combines the magnetic bead of nucleic acid in hole position 5, is transferred to hole position 6；
(13) the absorption of bar magnet magnetosheath combines the magnetic bead of nucleic acid, is transferred to hole position 6；
(14) bar magnet magnetosheath declines jointly, and the magnetic bead for having adsorbed nucleic acid is discharged to eluent；
(15) magnetosheath is moved up and down, and nucleic acid is eluted in hole position 6 to eluent；
(16) the magnetic bead after release is adsorbed and separated by bar magnet magnetosheath.
, but those skilled in the art once know basic creation although preferred embodiments of the present invention have been described
Property concept, then can make other change and modification to these embodiments.So, appended claims are intended to be construed to include excellent
Select embodiment and fall into having altered and changing for the scope of the invention.
Obviously, those skilled in the art can carry out the essence of various changes and modification without departing from the present invention to the present invention
God and scope.So, if these modifications and variations of the present invention belong to the scope of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to comprising including these changes and modification.
Claims (10)
1. a kind of extraction element, it is characterised in that including pedestal（1）, heater（5）, kit（14）, filter core（3-2）, wind
Fan（3-1）, movable mechanism（8）, shell（2）, magnetosheath（33）, magnetosheath frame（7）, bar magnet（140）With bar magnet frame（6）,
The heater（5）It is fixedly connected on the pedestal（1）,
The kit（14）It is arranged at the heater（5）On,
The movable mechanism（8）One end be fixedly connected on the pedestal（1）, the magnetosheath frame（7）It is fixedly connected on described
Movable mechanism（8）The other end, the movable mechanism（8）For controlling the bar magnet frame（6）With magnetosheath frame（7）Exist respectively
The movement of horizontal direction and vertical direction,
The shell（2）It is located at the kit（14）, magnetosheath（33）, magnetosheath frame（7）, movable mechanism（8）, filter core（3-
2）And fan（3-1）Outside, and in the shell（2）With the pedestal（1）Between form accommodating cavity（32）,
The filter core（3-2）It is fixedly connected on the accommodating cavity（32）Outer wall on, also, the filter core（3-2）It is completely covered
State accommodating cavity in residence（32）,
The fan（3-1）It is arranged on the filter core（3-2）Top,
The accommodating cavity（32）Side wall be provided with the first air admission hole（31a or 31b）, air-flow passes through the first described air admission hole
（31a or 31b）, accommodating cavity（32）, filter core（3-2）, fan（3-1）Realize circulation,
The magnetosheath frame（7）It is provided with the first perforating, the magnetosheath（33）Institute is arranged in by the first described perforating
State magnetosheath frame（7）On,
The bar magnet frame（6）Provided with second of perforating, the bar magnet（140）Institute is arranged in by second of perforating
State bar magnet frame（6）On,
The bar magnet（140）With the magnetosheath（33）Position in space is corresponding so that the bar magnet（140）It can extend into
The magnetosheath（33）In.
2. extraction element according to claim 1, it is characterised in that also including foot pad（16）, the foot pad（16）It is fixed
It is connected to the pedestal（1）Bottom, for the foot pad to be made（16）Material be selected from rubber.
3. extraction element according to claim 1, it is characterised in that the heater（5）Including hot well（5-1）, heat
Source, the kit（14）It is removable installed in the hot well（5-1）Top, the thermal source is used for the hot well（5-
1）Heat energy is provided.
4. extraction element according to claim 1, it is characterised in that also including at least one pair of elastomer（5-3）, it is described to add
Thermal（5）Top be provided with heating panel（5-2）, at least one pair of described elastomer（5-3）It is arranged at the heating panel（5-
2）Two ends, when the kit（14）It is placed in the heating panel（5-2）On when, the elastomer（5-3）Holding is in institute
State the two ends of kit, also, the elastomer（5-3）In compressive state.
5. extraction element according to claim 1, it is characterised in that also including magnetosheath frame（7-1）, magnetosheath（33）, precompressed
Ball（7-4）,
The magnetosheath frame（7-1）It is fixedly connected on the magnetosheath frame（7）,
The magnetosheath（33）Wear and penetrate in the magnetosheath frame（7-1）,
The magnetosheath frame（7-2）It is provided with storage tank, the magnetosheath（33）Radial dimension ＞ described in storage tank radial dimension,
So that, when the magnetosheath（33）With the magnetosheath frame（7-1）During cooperation, the magnetosheath（33）With the magnetosheath frame（7-1）Between
It is interference fit,
The pre- pressure ball（7-4）It is arranged at the storage tank and magnetosheath（33）Between gap in.
6. extraction element according to claim 1, it is characterised in that also including shell（2）,
The shell（2）The pedestal is fixedly connected on by its base edge（1）,
The shell（2）Side wall provided with second air admission hole（35a or 35b）, second of air admission hole（35a or 35b）
With the first described air admission hole（31a or 31b）Position it is corresponding,
The shell（2）Side wall on be additionally provided with venthole（34a or 34b）, the venthole（34a or 34b）So that described
Fan（3-1）The gas outflow pumped out.
7. extraction element according to claim 1, it is characterised in that the movable mechanism includes moving horizontally component
（A）With vertical shift component（B）；
It is described to move horizontally component（A）Including stepper motor（107）, the first connecting plate（102）, the first limiting plate（131）, first
Sliding block（106）, the second limiting plate（132）, screw rod（105）With the first feed rod（103）,
First connecting plate（102）, the first limiting plate（131）With the second limiting plate（132）It is vertically connected at the pedestal
（1）,
The stepper motor（107）It is fixedly connected on first connecting plate（102）, the stepper motor（107）Output shaft
It is fixedly connected on the screw rod（105）One end, the screw rod（105）The other end and first sliding block（106）Between structure
Into screw thread pair, first sliding block（106）Along first feed rod（103）Axially at least provided with first through hole（134）, institute
State the first feed rod（103）With first sliding block（106）Between pass through first through hole（134）Constitute sliding pair so that described the
One sliding block（106）Can be in first limiting plate（131）With the second limiting plate（132）Between slide；
The vertical shift component（B）It is fixedly connected on first sliding block（106）；
The bar magnet frame（6）With magnetosheath frame（7）It is connected to the vertical shift component（B）So that the bar magnet frame（6）With
Magnetosheath frame（7）Separately in the vertical direction is moved.
8. extraction element according to claim 7, it is characterised in that the vertical shift component B includes the second connecting plate
（108）, the first electric rotating machine（109）, the second electric rotating machine（133）, the first tape handler（110）, the second tape handler
（118）, clutch shaft bearing（112）, second bearing（114）, 3rd bearing（119）, fourth bearing（123）, slide rail（125）, second
Sliding block（126）, the 3rd sliding block（127）, the first connector（116）With the second connector（122）,
The clutch shaft bearing（112）, second bearing（114）, 3rd bearing（119）, fourth bearing（123）It is embedded at respectively described
Second connecting plate（108）, wherein, the clutch shaft bearing（112）And second bearing（114）It is described in same vertical straight line
3rd bearing（119）And fourth bearing（123）In same vertical straight line；
First electric rotating machine（109）Output shaft pass through the clutch shaft bearing（112）The first band is fixedly connected on to pass
Motivation structure（110）The first driving wheel（111）；Second electric rotating machine（133）Output shaft pass through second V belt translation
Mechanism（118）The second driving wheel（139）；
The slide rail（125）Vertically it is fixedly connected on second connecting plate（108）On, second sliding block（126）、
3rd sliding block（127）Respectively with the slide rail（125）Constitute sliding pair；
First connector（116）One end be fixedly connected on second sliding block（126）, first connector（116）
The other end be fixedly connected on first tape handler（110）The first conveyer belt（117）；
Second connector（122）One end be fixedly connected on the 3rd sliding block（127）, second connector（122）
The other end be fixedly connected on second tape handler（118）The second conveyer belt（136）；
First connector（116）Pass through its external part（128）It is fixedly connected on the bar magnet frame（6）；
Second connector（122）Pass through its external part（129）It is fixedly connected on the magnetosheath frame.
9. extraction element according to claim 8, it is characterised in that
First conveyer belt（117）It is provided with the first engaging tooth（137）, first connector（116）The other end be provided with
Second engaging tooth, first connector（116）Pass through first engaging tooth（137）, the engagement between the second engaging tooth consolidates
Fixed connection；
Second conveyer belt（136）It is provided with the 3rd engaging tooth（138）, second connector（122）The other end be provided with
4th engaging tooth, second connector（122）Pass through the 3rd engaging tooth（138）, the engagement between the 4th engaging tooth consolidates
Fixed connection.
10. extraction element according to claim 7, it is characterised in that also including the 3rd connecting plate（101）, the described 3rd
Connecting plate（101）Pass through one face and the pedestal（1）It is fixedly connected, first connecting plate（102）, the first limiting plate
（131）With the second limiting plate（132）It is vertically connected at the 3rd connecting plate（101）Another face；
Preferably, the extraction element also includes the second feed rod（104）, second feed rod（104）With first feed rod
（103）It is parallel,
First sliding block（106）Along second feed rod（104）Axial direction be additionally provided with the second through hole（135）, second light
Thick stick（104）With first sliding block（106）Between pass through second through hole（135）Constitute sliding pair；
Preferably, the extraction element also includes uviol lamp（19）, the uviol lamp（19）It is described by sending ultraviolet light
Accommodating cavity（32）Gnotobasis is provided；
Preferably, the extraction element also includes power outlet（130）, the power line of the consuming parts in the extraction element
Power connection end be respectively arranged at the power outlet（130）It is interior；
Preferably, the extraction element also includes the first locating part（141）,
In second connecting plate（108）On, along first tape handler（110）The first conveyer belt（117）Motion
Direction is provided with the first elongate slot（144）So that it is arranged at first connector（116）The plush copper of the other end（142）It is placed in
First elongate slot（144）It is interior；
First locating part（141）Across first elongate slot（144）Set, when first connector（116）Upwards
When moving to extreme position, first connector is arranged at（116）The plush copper of the other end（142）By first locating part
（141）Backstop；
Preferably, the extraction element also includes the second locating part（143）,
In second connecting plate（108）On, along second tape handler（118）The second conveyer belt（136）Motion
Direction is provided with the second elongate slot so that be arranged at second connector（122）It is narrow that the plush copper of the other end is placed in described second
In elongated slot；
Second locating part（143）Set across second elongate slot, when second connector（122）Move upward to
During extreme position, second connector is arranged at（122）The plush copper of the other end is by second locating part（143）Backstop.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710636784.0A CN107287194A (en) | 2017-07-31 | 2017-07-31 | Extraction element |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710636784.0A CN107287194A (en) | 2017-07-31 | 2017-07-31 | Extraction element |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107287194A true CN107287194A (en) | 2017-10-24 |
Family
ID=60102908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710636784.0A Pending CN107287194A (en) | 2017-07-31 | 2017-07-31 | Extraction element |
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| Country | Link |
|---|---|
| CN (1) | CN107287194A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108507859A (en) * | 2018-05-31 | 2018-09-07 | 孔治 | The automatic Paramagnetic particles adsorption separation device of sample subregion |
| US11634703B2 (en) * | 2018-03-02 | 2023-04-25 | Psomagen, Inc. | Method and system for high-throughput particle handling by use of magnetic fields and device |
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| WO2011136624A3 (en) * | 2010-04-30 | 2012-03-01 | (주)바이오니아 | Automatic biological sample purification device having a magnetic-field-applying unit, a method for extracting a target substance from a biological sample, and a protein expression and purification method |
| CN203715614U (en) * | 2013-12-30 | 2014-07-16 | 西安天隆科技有限公司 | Closed extraction apparatus for single sample nucleic acid |
| CN107034125A (en) * | 2017-06-09 | 2017-08-11 | 王炜 | Extraction element |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2011136624A3 (en) * | 2010-04-30 | 2012-03-01 | (주)바이오니아 | Automatic biological sample purification device having a magnetic-field-applying unit, a method for extracting a target substance from a biological sample, and a protein expression and purification method |
| CN203715614U (en) * | 2013-12-30 | 2014-07-16 | 西安天隆科技有限公司 | Closed extraction apparatus for single sample nucleic acid |
| CN107034125A (en) * | 2017-06-09 | 2017-08-11 | 王炜 | Extraction element |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| US11634703B2 (en) * | 2018-03-02 | 2023-04-25 | Psomagen, Inc. | Method and system for high-throughput particle handling by use of magnetic fields and device |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108507859A (en) * | 2018-05-31 | 2018-09-07 | 孔治 | The automatic Paramagnetic particles adsorption separation device of sample subregion |
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Application publication date: 20171024 |