CN201176432Y - Full automatic instrument for extracting nucleic acid - Google Patents
Full automatic instrument for extracting nucleic acid Download PDFInfo
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- CN201176432Y CN201176432Y CNU2007200769207U CN200720076920U CN201176432Y CN 201176432 Y CN201176432 Y CN 201176432Y CN U2007200769207 U CNU2007200769207 U CN U2007200769207U CN 200720076920 U CN200720076920 U CN 200720076920U CN 201176432 Y CN201176432 Y CN 201176432Y
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Abstract
The utility model relates to a full-automatic nucleic acid extractor, which is composed of a control module and an operation module, wherein the control module is composed of a touching screen and a main computer, the operation module is composed of a conveying system, a heating system, a magnetic bead transferring system, a reagent discharging system, a mixing pump system and a cleaning pump. The full-automatic nucleic acid extractor is matched with a closed nucleic acid (or protein) extracting box, and orders relative software programs of a microcomputer system to operate through the touching screen, transfers various signals to a real-time control system and assigns the signals to other systems, thereby the operations of transferring the closed nucleic acid (or protein) extracting box, mixing the reagent, heating, transferring the magnetic beads and the cleaning are completed. The utility model has beneficial effects that the extraction of the nucleic acid (or protein) can be accurately completed under the closed state, the output and the purity quotient are high, simultaneously the whole process has no need of adding any reagent, and the cross-infection between samples, the environment pollution and the personnel infection are avoided.
Description
Affiliated technical field
The utility model relates to a kind of nucleic acid (or albumen) automatic extracting instrument device, is used for isolating nucleic acid (or albumen) from several samples material such as blood, tissue, cell.
Background technology
In gene and protein engineering research field, all need to carry out nucleic acid (or albumen) and extract operation, at present, adopt the magnetic bead isolation technique at home, be that manual operations also is to use automatic equipment all need add multiple different reagent respectively to sample under the state of opening (the air contact is arranged), and repeatedly carry out to mix, precipitation, heating, the operation of filtering, extracting supernatant liquor, problem such as exist personnel to infect when therefore carrying out this experiment or sample contamination probability height, waste consumptive material, inefficiency, complex operation, dna purity be low.
Summary of the invention
Extract and to infect in the test experience or pollution probability height, waste consumptive material, inefficiency, complex operation, shortcoming that dna purity is low in order to overcome nucleic acid (or albumen), the utility model provides and a kind ofly can not only shorten the experimental implementation time, accurately finishing nucleic acid (or albumen) extracts, the most important thing is to make operations under enclosed state, to finish, can not exist leakage or when heating gas evolution situation, reach and stop sample contamination and personnel are subjected to the purpose that sample infects.
Full-automatic nucleic acid extraction instrument is made up of control module and operation module.Control module is made of touch screen, host computer; The operation module is made up of real-time control system, cooling system, transfer system, heating system, magnetic bead transfer system, reagent release mixing pump system, scavenging pump system.A control module can be joined a plurality of operation modules simultaneously.
In the operation module, real-time control system is positioned at the top in the cabinet, and the every programmed instruction that sends by the mainboard receiving control system is controlled the operation of other each system; Transfer system carries out tangential movement by step motor, horizontal bearing control transfer station; Have two group reagents to discharge the mixing pump system, one group above transfer system, another group is then parallel with transfer system, by step motor, vertically bearing control piston rod moves up and down and locatees; Heating control system moves up and down and locatees by step motor, vertical bearing control heater below transfer system; The scavenging pump system is parallel with the reagent mix pump system, moves up and down and locatees by step motor, vertical bearing control piston rod; The magnetic bead transfer system is parallel with heating system, moves up and down, locatees and rotate by step motor, vertical bearing control bar magnet, simultaneously by the rotation of gear-bearing control bar magnet; Cooling system is positioned at the rear of cabinet, carries out radiating and cooling by fan.
After adopting such scheme, full-automatic nucleic acid extraction instrument and closed nucleic acid (or albumen) extract the supporting use of box, assign instruction operation microcomputer system relevant software programs by touch-screen, transmit various signals and be assigned to other each system again to real-time control system, with the operation that transmission, release mix reagent, heating, the magnetic bead of finishing closed nucleic acid (or albumen) extraction box shifts, cleans, whole process is finished under closed state fully.
The beneficial effects of the utility model are, accurately finish nucleic acid (or albumen) and extract under enclosed state, not only reach the high yield high purity, and whole process need not to add any reagent more simultaneously, avoid sample cross infection, contaminate environment and personnel to infect.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Accompanying drawing one: full-automatic nucleic acid extraction instrument front view
Accompanying drawing two: full-automatic nucleic acid extraction instrument reagent discharges mixing pump system front view
Accompanying drawing three: full-automatic nucleic acid extraction instrument heating control system front view
Accompanying drawing four: full-automatic nucleic acid extraction instrument scavenging pump system front view
Accompanying drawing five: full-automatic nucleic acid extraction instrument magnetic bead transfer system front view
1. touch-screens among the figure; 2. host computer; 3. operation module; 4. stack fixer; 5. indicator panel; 6. delivery platform security device; 7. delivery platform; 8. delivery platform door; 9. piston rod in parallel; 10. vertical sliding surface bearing; 11. horizontal anomalous movement motor; 12. CD-ROM drive motor; 13. level is handled bearing; 14. well heater; 15. vertical bearing; 16. heating junctor; 17. transmitter; 18. motor; 19. parallel lines drive shaft and bearing; 20. vertical pump active motors; 21. vertical pump; 22. position sensor; 23. bar magnet; 24. sea line bearing; 25. gear; 26. driving cog wheel carrier; 27. active motor; 28. gear roller bearing; 29. line motor;
Specific implementation method
In Fig. 1, touch screen (1) constitutes control module with host computer (2), control module sends instructions to operation module (3), a plurality of operation modules (3) can be stacked together by stacking fixer (4), indicator panel (5) can show the running status of operation module (3), and delivery platform security device (6), delivery platform (7), delivery platform door (8) are formed transfer system.Under step motor, horizontal bearing effect, delivery platform (7) carries out the front and back tangential movement, according to relevant operating instruction closed nucleic acid (or albumen) is extracted box and delivers to corresponding position.
In Fig. 2, handle bearing (13) by piston rod in parallel (9), vertical sliding surface bearing (10), horizontal anomalous movement motor (11), CD-ROM drive motor (12), level and form reagent release mixing pump system.Full-automatic nucleic acid extraction instrument has two group reagents to discharge the mixing pump system, one group above transfer system, another group is then parallel with transfer system, handling bearing (13) control piston rod (9) by vertical sliding surface bearing (10), horizontal anomalous movement motor (11), CD-ROM drive motor (12), level moves up and down and locatees, act on closed nucleic acid (or albumen) according to relevant operating instruction and extract box, reach the purpose of release and mix reagent.Reagent discharges the quantity of the piston rod in parallel (9) in the mixing pump system not to be limit, and all can to 384 for 1.
In Fig. 3, well heater (14), vertical bearing (15), heating junctor (16), transmitter (17), motor (18) are formed heating control system.Heating control system is below transfer system, by vertical bearing (15), heating junctor (16), transmitter (17), motor (18) control heater (14) moves up and down and locate, act on closed nucleic acid (or albumen) according to relevant operating instruction and extract box, reach the purpose that adds thermal agent.The quantity of the well heater in the heating control system (14) is not limit, and all can to 384 for 1.
In Fig. 4, parallel lines drive shaft and bearing (19), vertical pump active motors (20), vertical pump (21), position sensor (22) are formed the scavenging pump system.The scavenging pump system is parallel with the reagent mix pump system, move up and down and locate by parallel lines drive shaft and bearing (19), vertical pump active motors (20), position sensor (22) control vertical pump (21), act on closed nucleic acid (or albumen) according to relevant operating instruction and extract box, reach the purpose that release reagent cleans.The quantity of the vertical pump in the scavenging pump system (21) is not limit, and all can to 384 for 1.
In Fig. 5, bar magnet (23), horizontal spools (24), gear (25), driving cog wheel carrier (26). initiatively motor (27), gear roller bearing (28), line motor (29) are formed the magnetic bead transfer system.The magnetic bead transfer system is parallel with heating system, move up and down, locate and rotate by horizontal spools (24), gear (25), driving cog wheel carrier (26), active motor (27), gear roller bearing (28), line motor (29) control bar magnet (23), act on closed nucleic acid (or albumen) according to relevant operating instruction and extract box, reach magnetic bead is extracted the purpose that inner each chamber of box shifts at closed nucleic acid (or albumen).The quantity of the bar magnet in the magnetic bead transfer system (23) is not limit, and all can to 384 for 1.
Claims (9)
1, a kind of full-automatic nucleic acid extraction instrument, form by control module and operation module, it is characterized in that: a control module links to each other with one or more operation modules, control module is made of touch screen and host computer, and the operation module is made up of real-time control system, cooling system, transfer system, heating system, magnetic bead transfer system, reagent release mixing pump system, scavenging pump system.
2, full-automatic nucleic acid extraction instrument according to claim 1 is characterized in that touch screen and host computer constitute control module, and control module sends instructions to the operation module.
3, full-automatic nucleic acid extraction instrument according to claim 1 is characterized in that a control module links to each other with one or more operation modules, by stacking fixer a plurality of operation modules is stacked together.
4, full-automatic nucleic acid extraction instrument according to claim 1, it is characterized in that delivery platform security device, delivery platform, delivery platform door, step motor, horizontal bearing composition transfer system, carry out tangential movement, location by step motor, horizontal bearing control delivery platform, closed nucleic acid or protein extraction box are delivered to specified location.
5, full-automatic nucleic acid extraction instrument according to claim 1 is characterized in that full-automatic nucleic acid extraction instrument has two group reagents to discharge the mixing pump system, one group above transfer system, another group is parallel with transfer system,
6, full-automatic nucleic acid extraction instrument according to claim 1, it is characterized in that reagent discharges the mixing pump system and moves up and down, locatees by vertical sliding surface bearing, horizontal anomalous movement motor, CD-ROM drive motor, level manipulation bearing control piston rod, discharge and mix reagent in closed nucleic acid or the protein extraction box, the quantity of piston rod in parallel is not limit, and all can to 384 for 1.
7, full-automatic nucleic acid extraction instrument according to claim 1, it is characterized in that heating control system is below transfer system, form by well heater, vertical bearing, heating junctor, transmitter, motor, by vertical bearing, heating junctor, transmitter, the motor control heater moves up and down and locate, reagent in closed nucleic acid or the protein extraction box is heated, and the quantity of well heater all can from 1 to 384.
8, full-automatic nucleic acid extraction instrument according to claim 1, it is characterized in that the scavenging pump system is parallel with the reagent mix pump system, form by parallel lines drive shaft and bearing, vertical pump active motors, vertical pump, position sensor, move up and down and locate by parallel lines drive shaft and bearing, vertical pump active motors, position sensor control vertical pump, the quantity of vertical pump all can from 1 to 384.
9, full-automatic nucleic acid extraction instrument according to claim 1, it is characterized in that the magnetic bead transfer system is parallel with heating system, by bar magnet, horizontal spools, gear, driving cog wheel carrier., initiatively motor, gear roller bearing, line motor are formed, by horizontal spools, gear, driving cog wheel carrier. initiatively motor, gear roller bearing, line motor control bar magnet move up and down, locate and rotate, and the quantity of bar magnet all can from 1 to 384.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200769207U CN201176432Y (en) | 2007-12-21 | 2007-12-21 | Full automatic instrument for extracting nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007200769207U CN201176432Y (en) | 2007-12-21 | 2007-12-21 | Full automatic instrument for extracting nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201176432Y true CN201176432Y (en) | 2009-01-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007200769207U Expired - Lifetime CN201176432Y (en) | 2007-12-21 | 2007-12-21 | Full automatic instrument for extracting nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201176432Y (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755761A (en) * | 2011-04-29 | 2012-10-31 | 博奥生物有限公司 | Biomolecule extraction device |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN104059848A (en) * | 2014-06-26 | 2014-09-24 | 广州冠科生物科技有限公司 | Uniform mixing device for nucleic acid extraction |
| WO2016161739A1 (en) * | 2015-04-09 | 2016-10-13 | 余家昌 | Reagent cartridge provided with magnetic bead transferring structure |
| CN107831682A (en) * | 2017-11-17 | 2018-03-23 | 广州瑞能医学科技有限公司 | A kind of instrument for extracting nucleic acid intelligent control method |
| CN107988045A (en) * | 2018-01-08 | 2018-05-04 | 深圳市朗司医疗科技有限公司 | A kind of full-automatic nucleic acid purification system based on paramagnetic particle method |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN110627864A (en) * | 2019-11-07 | 2019-12-31 | 重庆大学 | Automatic extraction method of cell components |
| WO2020181825A1 (en) * | 2019-03-12 | 2020-09-17 | 陈辉 | Nucleic acid extraction apparatus |
| WO2023151185A1 (en) * | 2022-02-08 | 2023-08-17 | 深圳潘道医学检验实验室 | Automatic nucleic acid extractor |
-
2007
- 2007-12-21 CN CNU2007200769207U patent/CN201176432Y/en not_active Expired - Lifetime
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755761A (en) * | 2011-04-29 | 2012-10-31 | 博奥生物有限公司 | Biomolecule extraction device |
| CN102755761B (en) * | 2011-04-29 | 2014-07-09 | 博奥生物集团有限公司 | Biomolecule extraction device |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103756871B (en) * | 2013-12-30 | 2015-04-22 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN104059848A (en) * | 2014-06-26 | 2014-09-24 | 广州冠科生物科技有限公司 | Uniform mixing device for nucleic acid extraction |
| WO2016161739A1 (en) * | 2015-04-09 | 2016-10-13 | 余家昌 | Reagent cartridge provided with magnetic bead transferring structure |
| CN107831682A (en) * | 2017-11-17 | 2018-03-23 | 广州瑞能医学科技有限公司 | A kind of instrument for extracting nucleic acid intelligent control method |
| CN107988045A (en) * | 2018-01-08 | 2018-05-04 | 深圳市朗司医疗科技有限公司 | A kind of full-automatic nucleic acid purification system based on paramagnetic particle method |
| CN107988045B (en) * | 2018-01-08 | 2024-01-19 | 深圳市朗司医疗科技有限公司 | Full-automatic nucleic acid purification system based on magnetic bead method |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| WO2020181825A1 (en) * | 2019-03-12 | 2020-09-17 | 陈辉 | Nucleic acid extraction apparatus |
| CN110627864A (en) * | 2019-11-07 | 2019-12-31 | 重庆大学 | Automatic extraction method of cell components |
| WO2023151185A1 (en) * | 2022-02-08 | 2023-08-17 | 深圳潘道医学检验实验室 | Automatic nucleic acid extractor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: KAISHENG BIOTECH (SUZHOU) CO., LTD. YU JIACHANG |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20100322 Address after: 200021 Shanghai Fuxing Road No. 1 Shenneng International Building room 1603 Patentee after: Kai Sheng Biotechnology (Shanghai) Co., Ltd. Address before: 200021 Shanghai Fuxing Road No. 1 Shenneng International Building room 1603 Patentee before: Kai Sheng Biotechnology (Shanghai) Co., Ltd. Patentee before: Kai Sheng Biotechnology (Suzhou) Co., Ltd. Patentee before: Yu Jiachang |
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| ASS | Succession or assignment of patent right |
Owner name: KAISHENG BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: KAISHENG BIOTECH (SHANGHAI) CO., LTD. Effective date: 20100406 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 200021 ROOM 1603, SHENNENG INTERNATIONAL BUILDING, NO.1, FUXING MIDDLE ROAD, SHANGHAI CITY TO: MAILBOX 4301,ROAD TOWN, TORTOLA, VIRGIN ISLANDS(BRITISH) |
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| TR01 | Transfer of patent right |
Effective date of registration: 20100406 Address after: The British Virgin Islands of Tortola Rodez P.O. Box No. 4301 Patentee after: Progentech Address before: 200021 Shanghai Fuxing Road No. 1 Shenneng International Building room 1603 Patentee before: Kai Sheng Biotechnology (Shanghai) Co., Ltd. |
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| ASS | Succession or assignment of patent right |
Owner name: LUMINEX CORP. (US) Free format text: FORMER OWNER: PROGENTECH Effective date: 20130724 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130724 Address after: texas Patentee after: Luminex Corp. (US) Address before: The British Virgin Islands of Tortola Rodez P.O. Box No. 4301 Patentee before: Progentech |
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| CX01 | Expiry of patent term |
Granted publication date: 20090107 |
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| CX01 | Expiry of patent term |