CN104059848A - Uniform mixing device for nucleic acid extraction - Google Patents
Uniform mixing device for nucleic acid extraction Download PDFInfo
- Publication number
- CN104059848A CN104059848A CN201410302327.4A CN201410302327A CN104059848A CN 104059848 A CN104059848 A CN 104059848A CN 201410302327 A CN201410302327 A CN 201410302327A CN 104059848 A CN104059848 A CN 104059848A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- mixing device
- gear
- bar magnet
- erecting frame
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a uniform mixing device for nucleic acid extraction. The uniform mixing device comprises a mounting rack, a driving motor, a driving gear, multiple driven gears, and a magnetic bar sleeve which corresponds to the multiple driven gears one by one, wherein a body part of the driving motor is fixed on the mounting rack; the multiple driven gears and the driving gear are pivoted on the mounting rack and are arranged along the line; the driving gear, a driven gear adjacent to the driving gear, and adjacent two driven gears are matched with one another in a meshed mode; the driving gear is synchronously coupled to a rotating shaft of the driving motor; the magnetic bar sleeve is arranged below the mounting rack, and at least one stirring blade is arranged at the outer periphery of the magnetic bar sleeve; the top of the magnetic bar sleeve is synchronously coupled to the corresponding driven gears. The liquid can be prevented from splashing from the top of a test tube, and an aerosol phenomenon is reduced, so that cross infection is effectively reduced, and the sample detection accuracy is improved. Meanwhile, the uniform mixing efficiency is high, the uniform mixing device can be matched with a magnetic bead separation device, and rapid nucleic acid separation is realized.
Description
Technical field
The present invention relates to a kind of nucleic acid extraction evenly mixing device.
Background technology
In disease control, the field such as food safety, forensic identification, genomics, conventionally need to extract biological active substance (as DNA) as detecting, the object of research, the extraction of traditional biologically active substance, generally include cracking broken wall, purify, the operations such as washing, conventionally need to be by the reagent adding and sample blending in purification process, for example, while adopting magnetic bead separation method to purify, after in the reagent with magnetic bead is joined to sample, need to utilize pipettor to be inserted in sample tube, utilize the suction repeatedly of rifle head that vacuum pump controls pipettor, be the usually said suction product of drawing a design, so that reagent and sample mix fully, the evenly mixing device of this structure in actual use, owing to being difficult to control the extracting force of vacuum pump, therefore, when aspirated specimens, be easy to cause splashing of sample, thereby cause the cross infection of sample room, simultaneously, can produce aerosol in sample interior, affect the accuracy of sample detection.
Summary of the invention
For the deficiencies in the prior art, object of the present invention is intended to provide a kind of nucleic acid extraction evenly mixing device, and it can avoid splashing of sample, reduces aerosol phenomenon, thereby effectively reduces cross infection, improves the accuracy of sample detection.
For achieving the above object, the present invention adopts following technical scheme:
Nucleic acid extraction evenly mixing device, comprise erecting frame, drive-motor, driving toothed gear, a plurality of follower gears, and with a plurality of follower gears bar magnet cover one to one, the body partial fixing of drive-motor is on erecting frame, the plurality of follower gear and driving toothed gear are all articulated on erecting frame and along linear arrangement, driving toothed gear and the follower gear being adjacent, and two adjacent follower gears all coordinate in the mode of engagement, driving toothed gear is synchronously connected in the rotating shaft of drive-motor, bar magnet cover is arranged at the below of erecting frame, its outer peripheral edges are provided with at least one agitating vane, top ends and the follower gear corresponding with it of bar magnet cover synchronously connect.
Bar magnet cover is the hollow structure of upper end opening, is provided with the through hole of the upper end open communication of overlapping with bar magnet on follower gear.
The upper surface of erecting frame is fixed with mount pad, and the body of drive-motor is fixed on the upper surface of mount pad, and driving toothed gear is arranged on holding in gap of forming between mount pad and erecting frame.
The diameter of driving toothed gear and a plurality of follower gears all equates.
Beneficial effect of the present invention is:
Than prior art, the present invention adopts bar magnet cover stirred sample and reagent, reagent and sample are mixed fully, and it adopts churned mechanically mode, can avoid liquid to splash from the top of test tube, reduce aerosol phenomenon, thereby effectively reduce cross infection, improve the accuracy of sample detection, simultaneously, than existing employing pipettor, inhale the mode of beating, its speed mixing is higher.In addition, the present invention can coordinate magnetic bead separating device, realizes the sharp separation of nucleic acid.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is use view of the present invention;
Wherein: 10, erecting frame; 20, drive-motor; 21, driving toothed gear; 22, mount pad; 30, follower gear; 31, through hole; 40, bar magnet cover.
Embodiment
Below, by reference to the accompanying drawings and embodiment, the present invention is described further:
As shown in Figure 1, for a kind of nucleic acid extraction evenly mixing device of the present invention, it comprises erecting frame 10, drive-motor 20, driving toothed gear 21, a plurality of follower gears 30 and a plurality of respectively with follower gear 30 bar magnet cover 40 one to one, wherein, erecting frame 10 is platy structure, the body partial fixing of drive-motor 20 is on erecting frame 10, and the rotating shaft of drive-motor 20 is along short transverse to downward-extension, 21 of driving toothed gears are synchronously connected in the rotating shaft of drive-motor 20, concrete is, upper surface at erecting frame 10 is fixed with a mount pad 22, the body of drive-motor 20 is fixed on mount pad 22, between mount pad 22 and erecting frame 10, form the gap that holds that a width is slightly larger than driving toothed gear 21 thickness, driving toothed gear 21 is arranged in this and holds gap, above-mentioned a plurality of follower gears 30 and driving toothed gear 21 are be all articulated in the upper surface of erecting frame 10 and arrange along the length direction of erecting frame 10, driving toothed gear 21 and the follower gear 30 being adjacent, and two adjacent follower gears 30 are all that the mode meshing coordinates, thereby, when drive-motor 20 drives driving toothed gear 21 to rotate, above-mentioned a plurality of follower gears 30 rotate under the drive of driving toothed gear 21 simultaneously.Bar magnet cover 40 is all arranged at the below of erecting frame 10, the outer peripheral edges of this bar magnet cover 40 are at least provided with an agitating vane, the top ends of bar magnet cover 40 and the follower gear 30 corresponding with it synchronously connect, the top end part of bar magnet cover 40 can be to be installed in the draw-in groove of follower gear 30 lower surfaces, under the drive of above-mentioned follower gear 30, bar magnet cover 40 is around self central axis rotation.Above-mentioned bar magnet cover 40 is the hollow structure of upper end opening, on follower gear 30, be provided with the through hole 31 with the upper end open communication of bar magnet cover 40, referring to Fig. 2, the bar magnet cover 40 of hollow forms a bar magnet cover, after utilizing bar magnet cover 40 fully to mix by the sample in test tube with the reagent of magnetic bead, nucleic acid is combined with magnetic bead, utilize bar magnet from through hole 31, to be inserted into bar magnet cover 40 from top to bottom, the magnetic bead of being combined with nucleic acid is attracted to the surface of bar magnet cover 40, erecting frame 10 and bar magnet are synchronized with the movement and depart from from sample tube afterwards, realize the separated of nucleic acid and sample, finally bar magnet is being extracted out from bar magnet cover 40, realize the separated of nucleic acid and magnetic bead.
Above-mentioned driving toothed gear 21 and the diameter of a plurality of follower gear 30 all equate, thereby make the stir speed (S.S.) of a plurality of bar magnet covers 40 identical.
Than prior art, the present invention adopts bar magnet to overlap 40 stirred samples and reagent, reagent and sample are mixed fully, and it adopts churned mechanically mode, can avoid liquid to splash from the top of test tube, reduce aerosol phenomenon, thereby effectively reduce cross infection, improve the accuracy of sample detection, simultaneously, than existing employing pipettor, inhale the mode of beating, its speed mixing is higher.In addition, the present invention can coordinate magnetic bead separating device, realizes the sharp separation of nucleic acid.
To one skilled in the art, can make other various corresponding changes and deformation according to technical scheme described above and design, and within these all changes and deformation all should belong to the protection domain of the claims in the present invention.
Claims (4)
1. a nucleic acid extraction evenly mixing device, it is characterized in that, comprise erecting frame, drive-motor, driving toothed gear, a plurality of follower gears, and with a plurality of follower gears bar magnet cover one to one, the body partial fixing of drive-motor is on erecting frame, the plurality of follower gear and driving toothed gear are all articulated on erecting frame and along linear arrangement, driving toothed gear and the follower gear being adjacent, and two adjacent follower gears all coordinate in the mode of engagement, driving toothed gear is synchronously connected in the rotating shaft of drive-motor, bar magnet cover is arranged at the below of erecting frame, its outer peripheral edges are provided with at least one agitating vane, top ends and the follower gear corresponding with it of bar magnet cover synchronously connect.
2. nucleic acid extraction evenly mixing device as claimed in claim 1, is characterized in that, bar magnet cover is the hollow structure of upper end opening, is provided with the through hole of the upper end open communication of overlapping with bar magnet on follower gear.
3. nucleic acid extraction evenly mixing device as claimed in claim 1, it is characterized in that, the upper surface of erecting frame is fixed with mount pad, and the body of drive-motor is fixed on the upper surface of mount pad, and driving toothed gear is arranged on holding in gap of forming between mount pad and erecting frame.
4. nucleic acid extraction evenly mixing device as claimed in claim 1, is characterized in that, the diameter of driving toothed gear and a plurality of follower gears all equates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410302327.4A CN104059848A (en) | 2014-06-26 | 2014-06-26 | Uniform mixing device for nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410302327.4A CN104059848A (en) | 2014-06-26 | 2014-06-26 | Uniform mixing device for nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104059848A true CN104059848A (en) | 2014-09-24 |
Family
ID=51547783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410302327.4A Pending CN104059848A (en) | 2014-06-26 | 2014-06-26 | Uniform mixing device for nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104059848A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015180561A1 (en) * | 2014-05-27 | 2015-12-03 | 北京自由度科学机器有限公司 | Apparatus and method for magnetic bead method |
| CN105420074A (en) * | 2016-01-19 | 2016-03-23 | 山西振东基赛生物科技有限公司 | Multifunctional biological substance separating device |
| CN105456037A (en) * | 2015-08-14 | 2016-04-06 | 新昌县恒泰隆机械设备有限公司 | Oil homogenizing mechanism and capsule production line |
| CN107058062A (en) * | 2017-06-09 | 2017-08-18 | 苏州天隆生物科技有限公司 | A kind of rotary nucleic acid-extracting apparatus and its control method |
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| CN110093343A (en) * | 2019-05-08 | 2019-08-06 | 中国人民解放军军事科学院军事医学研究院 | A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid |
| CN110449199A (en) * | 2018-05-07 | 2019-11-15 | 斯塔戈诊断公司 | Clamping device for container |
| JP2021090420A (en) * | 2019-12-10 | 2021-06-17 | インダストリアル テクノロジー リサーチ インスティチュートＩｎｄｕｓｔｒｉａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｒｅｓｅａｒｃｈ Ｉｎｓｔｉｔｕｔｅ | Nucleic acid-extracting apparatus |
| CN115011467A (en) * | 2022-08-08 | 2022-09-06 | 深圳市华晨阳科技有限公司 | Automatic change nucleic acid extraction appearance |
| WO2023123571A1 (en) * | 2021-12-31 | 2023-07-06 | 博尔诚（北京）科技有限公司 | Fully-automatic nucleic acid methylation treatment device and treatment mechanism |
| US11733258B2 (en) | 2019-12-10 | 2023-08-22 | Industrial Technology Research Institute | Nucleic acid extracting device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6890492B1 (en) * | 1998-08-13 | 2005-05-10 | Symyx Technologies, Inc. | Parallel reactor with internal sensing and method of using same |
| CN201176432Y (en) * | 2007-12-21 | 2009-01-07 | 凯晟生物科技（上海）有限公司 | Full automatic instrument for extracting nucleic acid |
| CN203923174U (en) * | 2014-06-26 | 2014-11-05 | 广州冠科生物科技有限公司 | A kind of nucleic acid extraction evenly mixing device |
-
2014
- 2014-06-26 CN CN201410302327.4A patent/CN104059848A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6890492B1 (en) * | 1998-08-13 | 2005-05-10 | Symyx Technologies, Inc. | Parallel reactor with internal sensing and method of using same |
| CN201176432Y (en) * | 2007-12-21 | 2009-01-07 | 凯晟生物科技（上海）有限公司 | Full automatic instrument for extracting nucleic acid |
| CN203923174U (en) * | 2014-06-26 | 2014-11-05 | 广州冠科生物科技有限公司 | A kind of nucleic acid extraction evenly mixing device |
Non-Patent Citations (1)
| Title |
|---|
| 焦志等: "MP- 120工作站批量提取法医样本DNA", 《生命科学仪器》 * |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015180561A1 (en) * | 2014-05-27 | 2015-12-03 | 北京自由度科学机器有限公司 | Apparatus and method for magnetic bead method |
| CN105456037A (en) * | 2015-08-14 | 2016-04-06 | 新昌县恒泰隆机械设备有限公司 | Oil homogenizing mechanism and capsule production line |
| CN105420074A (en) * | 2016-01-19 | 2016-03-23 | 山西振东基赛生物科技有限公司 | Multifunctional biological substance separating device |
| CN105420074B (en) * | 2016-01-19 | 2018-06-29 | 山西振东基赛生物科技有限公司 | A kind of multifunctional bio material disconnecting gear |
| CN107058062B (en) * | 2017-06-09 | 2020-01-14 | 苏州天隆生物科技有限公司 | Rotary nucleic acid extraction device and control method thereof |
| CN107058062A (en) * | 2017-06-09 | 2017-08-18 | 苏州天隆生物科技有限公司 | A kind of rotary nucleic acid-extracting apparatus and its control method |
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| CN110449199A (en) * | 2018-05-07 | 2019-11-15 | 斯塔戈诊断公司 | Clamping device for container |
| CN110449199B (en) * | 2018-05-07 | 2023-03-10 | 斯塔戈诊断公司 | Clamping device for containers |
| CN110093343A (en) * | 2019-05-08 | 2019-08-06 | 中国人民解放军军事科学院军事医学研究院 | A kind of integrated portable nucleic acid-extracting apparatus and method for extracting nucleic acid |
| JP2021090420A (en) * | 2019-12-10 | 2021-06-17 | インダストリアル テクノロジー リサーチ インスティチュートＩｎｄｕｓｔｒｉａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｒｅｓｅａｒｃｈ Ｉｎｓｔｉｔｕｔｅ | Nucleic acid-extracting apparatus |
| JP7101746B2 (en) | 2019-12-10 | 2022-07-15 | インダストリアル テクノロジー リサーチ インスティチュート | Nucleic acid extractor |
| US11733258B2 (en) | 2019-12-10 | 2023-08-22 | Industrial Technology Research Institute | Nucleic acid extracting device |
| WO2023123571A1 (en) * | 2021-12-31 | 2023-07-06 | 博尔诚（北京）科技有限公司 | Fully-automatic nucleic acid methylation treatment device and treatment mechanism |
| CN115011467A (en) * | 2022-08-08 | 2022-09-06 | 深圳市华晨阳科技有限公司 | Automatic change nucleic acid extraction appearance |
| CN115011467B (en) * | 2022-08-08 | 2022-10-25 | 深圳市华晨阳科技有限公司 | Automatic change nucleic acid extraction appearance |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104059848A (en) | Uniform mixing device for nucleic acid extraction | |
| CN203923174U (en) | A kind of nucleic acid extraction evenly mixing device | |
| CN103789198B (en) | Full automatic instrument for extracting nucleic acid | |
| CN105733941A (en) | Magnetic bead separation device for nucleic acid extraction | |
| CN113210024B (en) | Continuous liquid inlet device based on PCR | |
| CN103992940B (en) | A kind of instrument extracting biologically active substance | |
| CN104263633B (en) | A kind of high-throughput biological specimen treatment unit based on Magneto separate | |
| CN203855572U (en) | Instrument for extracting active substance of organisms | |
| CN202182888U (en) | Liquid pumping and drainage driving mechanism of automatic sample-adding gun | |
| EP2944373B1 (en) | Device for suspending cells | |
| CN105733942A (en) | Device for nucleic acid extraction | |
| CN202777992U (en) | Sewage filtering device | |
| CN205635634U (en) | A device for nucleic acid extraction | |
| CN204747020U (en) | Medical science tube cleaning device for biochemistry | |
| CN208234955U (en) | A kind of rare earth processing stirring linkage extraction equipment | |
| CN206477001U (en) | biomolecule extraction device | |
| WO2023284844A1 (en) | Automatic extraction device and use method therefor | |
| CN106198105B (en) | A kind of horse blood plasma sampler | |
| CN203564994U (en) | Stirring device for processing glaze material | |
| CN213699681U (en) | Full-automatic agitating unit for nucleic acid extraction appearance | |
| CN202182895U (en) | Single-beam four-channel automatic sampling gun | |
| CN205635636U (en) | A magnetic beads sorting device for nucleic acid extraction | |
| CN105132283A (en) | Hot lid mechanism of fluorescent quantitative PCR (polymerase chain reaction) instrument | |
| CN210893870U (en) | Multi-channel reagent liquid changing device of dehydrator for pathological tissues | |
| CN205965113U (en) | Automatic solid phase extraction apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140924 |