CN105420074A - Multifunctional biological substance separating device - Google Patents
Multifunctional biological substance separating device Download PDFInfo
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- CN105420074A CN105420074A CN201610034026.7A CN201610034026A CN105420074A CN 105420074 A CN105420074 A CN 105420074A CN 201610034026 A CN201610034026 A CN 201610034026A CN 105420074 A CN105420074 A CN 105420074A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention provides a multifunctional biological substance separating device, and belongs to the technical field of biology. The multifunctional biological substance separating device comprises a shell, a main shaft pushing rod, a return device, a magnetic sleeve connecting ring and an electric device, wherein the main shaft pushing rod penetrates through the shell; two ends of the main shaft pushing rod both stretch out of the shell; one end of the main shaft pushing rod is provided with a magnetic post; the magnetic sleeve connecting ring is arranged on the main shaft pushing rod in a sleeving way; one end, which is positioned outside the shell, of the magnetic sleeve connecting ring is provided with a magnetic sleeve; the return device is arranged in the shell and is used for enabling the main shaft pushing rod to move towards the direction which is far away from the magnetic sleeve; the electric device is connected with the magnetic sleeve connecting ring and is used for enabling the magnetic sleeve connecting ring (comprising the magnetic sleeve arranged thereon) to do rotary motion. The multifunctional biological substance separating device provided by the invention is particularly suitable for being applied to a process of extracting a nucleic acid through a paramagnetic particle method, has the effect of enabling a magnetic bead to be quickly separated from a system (mostly a lysis solution or an eluent) therein the magnetic bead exists and is compact in structure and convenient in operation.
Description
Technical field
The present invention relates to biological technical field, in particular to a kind of multifunctional bio material disconnecting gear.
Background technology
At biological technical field, the operation that the separation for material is mainly taked at present has centrifugal, filtration, extraction, electrophoresis etc.These side's ratio juris are to be separated different materials by the weight of solid matter, the size of diameter.If whizzer separate substance is different by the quality (proportion) of solid matter in liquid or fluent meterial, produce different interfaces under the influence of centrifugal force and reach the object of separating substances; Filtration (comprise and sieving) is by the aperture of filter paper (filter membrane) and is filtered the diameter of material to reach the object of separating substances.In addition, oven dry, volatilization, extraction, electrophoresis etc. are also had to be all lean on physics or the principles of chemistry to reach separating substances object mostly.
For above-mentioned several traditional separation method, concrete, the application of centrifuging in biological substance separation is very extensive, and history is also very long; Its defect is that each centrifugal front centrifuge tube used must mark clear, otherwise centrifugal after cannot determine sample label.Mark centrifuge tube is very time-consuming, time especially sample size is many.In addition, the material of cardinal principle equal in quality can only be divided into a certain aspect (interface) by centrifuging, can not isolate equal quality but different bioactive material.
Filtration, extraction, electrophoresis etc. all exist can only by the separating substances of equal quality to a certain aspect, equal quality can not be isolated but different bioactive material, simultaneously for used equipment, also have that too loaded down with trivial details, the easy generation sample hose of operating process mislabels, operating process is difficult to aseptic technique, and the precision of separating substances is all difficult to control, and also cannot distinguish the defect of material according to biological activity.
In recent years, in the sepn process of cell, virus, protein, nucleic acid, the application of immunology principle and nanometer magnetic bead is very extensive, such as, extract nucleic acid magnet stand method used and automatic extracting instrument.For magnet stand method, its principle first allows separated material and nanometer magnetic bead reaction bonded in a certain container (as test tube) form a complex body, then after magnetic bead is adsorbed onto tube wall by the additional magnetic field of test tube, then by some method, waste liquid is removed.One of shortcoming is that operator must mark each test tube, then carries out next step operation; The time that usual separation sample needs is 30 minutes, and N number of sample must N*30 minute.It is very fast that two of shortcoming is that magnetic bead precipitates in a liquid, want to keep all substances in liquid to be in uniform state, each operating process must be added with a unnecessary action-revolve whirlpool instrument combination treatment, this adds increased operation steps, and greatly reduces operating speed.At every turn three of shortcoming, before using whirlpool instrument, must cover tightly test tube cap, otherwise cannot complete operation step.Visible, these shortcomings above all can not grow with each passing hour with the technology in modern biology field.
For automatic extracting instrument, on market, existing DNA automatization extraction equipment majority adopts the principle of immunology and nanometer magnetic bead combination to complete its separating substances process.Its principle of design is substantially similar with magnet stand method, only increases automatization manipulation and other heating function.One of its shortcoming is that the preparatory stage is complicated especially; Two of shortcoming is that data input is pretty troublesome, repeatedly must check and check before start-up routine; As long as three of shortcoming is have a step to do wrong, sample is just useless by the gross.Even if four of shortcoming is after starting, the automated operation time is also very long (wants 30-150 minute usually, reason is that some step needs dehydration), and all adopt in existing installation oven dry of heating, etc. the means such as to be dried, this just substantially prolongs the whole operating time.
As can be seen here, though the method that above-mentioned magnet stand method and DNA automatization are extracted compensate for a lot of deficiencies (as carried out separate substance according to biological activity) of traditional method, but because the structure design of existing installation and technical process have weak point, in operation steps be difficult to the advantage embodying modern biology new technique and modern project the time.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of multifunctional bio material disconnecting gear, and to solve, the operating process existed in biological substance sepn process of the prior art (comprise and adopt paramagnetic particle method to carry out the separation of cell, virus, protein, nucleic acid) is loaded down with trivial details, the technical problem of time and effort consuming and separation efficiency difference.
This multifunctional bio material disconnecting gear provided by the invention, especially be applicable to being applied to the process that paramagnetic particle method provides nucleic acid, it has the effect that magnetic bead and its can be made to there is system (mostly being lysate or elutriant) sharp separation, and this apparatus structure is compact, easy and simple to handle.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
The invention provides a kind of multifunctional bio material disconnecting gear, comprise housing, run through the main shaft push rod of described housing, returning device, magnetosheath shack and electric device;
The two ends of described main shaft push rod all protrude from described housing, and its one end is provided with magnetic post; Described magnetosheath shack is sheathed on described main shaft push rod, and described magnetosheath shack one end be in outside described housing is provided with magnetosheath (it is to be noted, in this application, main shaft push rod and magnetosheath shack are not relatively-stationary, main shaft push rod is through the central hollow section of magnetosheath shack, thus forms a kind of similar magnetosheath shack and be enclosed within structure on main shaft push rod).
Described returning device is arranged on described enclosure interior; And for making described main shaft push rod move to the direction away from described magnetosheath; Described electric device is connected with described magnetosheath shack, and for making described magnetosheath shack rotate.
For this multifunctional bio material disconnecting gear, its agent structure is housing, this housing is provided with the main shaft push rod run through by housing; The two ends of main shaft push rod all protrude from housing, and have one end to be provided with magnetic post.In addition, main shaft push rod is arranged with magnetosheath shack, and its one end (being in the one end outside housing) is provided with magnetosheath; Like this under the drive of electric device, magnetosheath shack and magnetosheath can rotate; And when applying reactive force to one end (not arranging magnetic post) of main shaft push rod, main shaft push rod and magnetic post thereof then can realize relative movement with housing, and under the effect of returning device, then there is return in main shaft push rod.Also namely main shaft push rod in the housing to-and-fro movement time, magnetic post is also reciprocating in magnetosheath, and magnetosheath and magnetosheath shack then rotate under the effect of electric device.
This biological substance tripping device being carried out in the process applied, being separated magnetic bead lysate (containing the magnetic bead adsorbing nucleic acid and lysate) as used it for; Magnetosheath is extend in mixed system, now push main shaft push rod, the end of magnetic post the is extend into bottom of magnetosheath; Under the effect of magnetic post, the magnetic bead in mixed system has been adsorbed on magnetosheath, and is removed, thus realizes the sharp separation of magnetic bead and mixed system.Further, because magnetosheath is rotary under the drive of electric device, in rotary course, magnetic bead remains on magnetosheath by the effect in magnetic field, and liquid is got rid of de-under the influence of centrifugal force, and magnetic bead is further separated with liquid.When after centrifugal completing, remove the power putting on main shaft push rod, main shaft push rod is return under the effect of returning device, drives magnetic post to leave from the bottom of magnetosheath; By the magnetic bead that adsorbs owing to losing the adsorption of magnetic force thus departing from from magnetosheath, and next step operation can be carried out.
Above-mentioned biological substance tripping device its operation process in, being separated of magnetic bead and mixed system can be realized, so can overcome the separation that from mixed system, liquid phase sucking-off existed in prior art thoroughly and magnetic bead be easy to sedimentation and separated defect.In addition, this device is by after Beads enrichment, centrifugal operation can be realized further, thus improve the effect of whole biological substance separation, defect that is centrifugal, that filter, extract the quality different properties separating substances such as the methods such as biological substance separation can not realize can be overcome.
For this device, in order to the effect of main shaft push rod returning quickly can be realized, inventing in the biological substance tripping device provided, preferably, in described housing, be also fixedly installed wedging position; Described returning device comprises first collar, second collar and the first spring;
Described first collar, the first spring and second collar are sheathed on described main shaft push rod respectively, described first collar and described wedging position offset, described second collar is fixedly connected with described main shaft push rod, and the two ends of described first spring offset with described first collar and described second collar respectively;
The one end described main shaft push rod being in described hull outside is provided with push rod parcel cover; One end that described push rod parcel cover is closed is wrapped in the end of described main shaft push rod, and open one end and described second collar offset.
Wedging position is fixedly installed in housing, and when pushing push rod parcel cover like this, multiple structures such as main shaft push rod and second collar are all along housing motion (magnetic post stretches to the bottom of magnetosheath); Because first set loop contacts is resisted against on wedging position, and wedging position is fixing, when second collar towards first collar direction motion time, the first spring is compressed.Now as the additional pressure of release, because second collar and main shaft push rod are fixing, so after the first spring puts on the second collar reactive force, further by the drive of main shaft push rod, thus realize the return of whole main shaft push rod.This kind of return mode adopts two collars being placed on main shaft push rod to realize, and the collar has the effect of location to main shaft push rod, and therefore return is effective, not easily makes main shaft push rod generation beat.
Preferably, described enclosure interior is also fixedly installed locating sleeve, and described locating sleeve is sheathed on described push rod parcel and overlaps the part be in described housing; The inwall of described locating sleeve and described push rod wrap up the outer wall overlapped and fit.Push rod parcel cover and housing position by locating sleeve, make main shaft push rod steady, smooth and easy in reciprocating process further.
Preferably, setting the second spring on the housing is also comprised and magnetosheath departs from push rod; One end of described second spring is fixing, and one end that the other end and described magnetosheath depart from push rod offsets on the housing, and the other end of described magnetosheath disengaging push rod extends to the opening part of the described housing passed by magnetic post.
Magnetosheath departs from push rod under the effect of external force, it is along housing motion, be specially the shell ends motion towards being provided with magnetosheath, it is in the process of motion, bottom can abut on magnetosheath, and then magnetosheath can be departed from, after magnetosheath departs from, under the effect of the second spring, magnetosheath departs from the return original position of push rod.
Optionally, also circuit card is provided with in described housing; Described circuit card is electrically connected with described electric device, and described circuit card is also provided with the motor adjusting knob and charge port that all protrude from described housing; And the press button described housing is also provided with for control circuit board power supply.
The integrated various circuit of circuit card, by motor adjusting knob, the output rating that can realize electric device regulates, and directly controls the slewing rate of magnetosheath shack and magnetosheath; Realize the speed adjustment of magnetosheath; And by charge port (for simplicity, can USB interface be arranged to), realize charging effect can to power supply (being inside located at housing); In addition, housing is also provided with the press button for control circuit board power supply.
Preferably, described hull outside is provided with a handle, and described handle one end is fixing on the housing, and the other end bends to the direction towards magnetic post.By the handle of peripheral hardware on housing, can realize successfully hanging, freeing thing material disconnecting gear on the one hand; On the other hand, because this handle is bending, be convenient to user and hold, very easy to use.
Preferably, described electric device is arranged on described enclosure interior, described magnetosheath shack is fixedly connected with a follower gear, described electric device is provided with a transmitting gear engaged with described follower gear.Electric device (specifically can be arranged to a motor, and when magnetosheath is single, can preferably be arranged in housing) be installed with transmitting gear, after electric device runs, transmitting gear rotates, and then the follower gear engaged with it is also rotated, and final magnetosheath swivel arrangement and the magnetosheath of driving rotates; The mode of this kind of transmission can realize the rotation of magnetosheath easily, easy to operate feasible.
When the sample of disposable process is more, the mode (namely each housing configures a magnetosheath) of single head obviously can not meet batch processing demand, therefore, multiple magnetosheath is preferably set, and realize controlling multiple magnetosheath by main shaft push rod simultaneously, magnetic post is very important, therefore, further preferably, described biological substance tripping device also comprises a bull link span; One end of described bull link span and described housing is socketed;
Described main shaft push rod comprises a transmission rod and Duo Gen follower lever; All follower levers are arranged side by side, and are fixed by a union lever, and described union lever is fixedly connected with described transmission rod; Described transmission rod, follower lever and union lever are all arranged on described bull link span; All described follower levers are all through a retaining plate, and its one end through described retaining plate is provided with magnetic post, and described retaining plate is fixedly installed on described bull link span; Each described follower lever is all arranged with magnetosheath shack; On each magnetosheath shack, one end is provided with magnetosheath, and the other end is all fixed with follower gear; Intermesh between two often adjacent follower gears, described electric device is arranged on described bull link span, and for driving described follower gear to rotate.
In the biological substance tripping device of above-mentioned " bull structure ", simple main shaft push rod in its main shaft push rod and " single head structure " is also inconsistent, in this scenario, main shaft push rod is divided into two parts, and the part be wherein in housing is transmission rod (being also the main shaft push rod in " single head structure "); The follower lever that hull outside is divided into many to be arranged side by side; Many transmission rods are fixed by a union lever, and transmission rod is then fixedly connected with union lever.Like this after applying reactive force to push rod parcel cover, transmission rod then promotes union lever and the motion of all follower levers, and the magnetic post being now arranged on each follower lever end is then also pushed and stretches to the bottom of the magnetosheath of its correspondence.
In addition, due to each follower lever being all arranged with magnetosheath shack; One end of each magnetosheath shack is provided with magnetosheath, and the other end is all fixed with gear; Intermesh between two often adjacent gears, electric device is arranged on described bull link span and (conveniently realizes multiple gear by an electric device to rotate simultaneously).After electric device runs, as long as drive in multiple gear gear to rotate can realize the rotation of all gear units.
Due on bull link span and the structure such as retaining plate, when main shaft push rod (transmission rod) moves, only all follower levers (comprising the magnetic post of its end), union lever motion, and gear unit, retaining plate and be geo-stationary between bull link span and housing.
The above-mentioned biological substance tripping device with " bull structure ", corresponding construction and the single head biological substance tripping device of its enclosure interior are basically identical, but multiple magnetosheaths that the biological substance tripping device of this bull has due to it (comprising magnetic post), it can carry out batch processing to the treatment solution arranged in advance simultaneously, thus improve processing efficiency, for a large amount of time has been saved in experiment.
Preferably, described transmission rod is all parallel with all described follower levers, and described union lever is vertical with described transmission rod.
Preferably, described magnetosheath is one end open, the cavity that one end is closed, and one end of its opening is set on described magnetosheath shack, and the one end closed arranges multiple projection.
After the multiple projection of the outer setting of one end that magnetosheath is closed, it facilitates magnetic bead to be adsorbed to its surface, the phenomenon that the magnetic bead that also can effectively prevent magnetosheath from assembling in the process rotated on the other hand is scattered on the one hand.
Preferably, the cecum outside surface of described magnetosheath can be V-arrangement asperities, U-shaped asperities, V-arrangement light face or U-shaped light face.In addition, magnetic post can be cylindrical, square column type, ellipse, pie or any shape can placed in tubular container inside.
The blind end (cecum) of magnetosheath can be V-arrangement, also can be U-shaped, and for increasing mixing effect, cecum can additional ' in-line ', ' cruciform ' projection.
Magnetosheath can by plastics, and rubber, glass or not magnetic any known materials are made, and it can be transparent, opaque or distinct colors.And the mode of connection between magnetosheath shack and electric device changeable (being not limited to rotate), namely magnetosheath can do and vibrate.
Compared with prior art, beneficial effect of the present invention is:
(1), this biological substance tripping device provided by the invention, it is applied to after biological substance is separated, and has and is separated thoroughly, the advantage that efficiency is high; And utilizing this device to carry out in the process be separated, this device can simplify operation greatly, overcomes the defect that in prior art, magnetic bead is easily absorbed and in magnetic bead, remaining water not easily removes.
(2), the total of this device arranges compact, easy to use, when magnetosheath rotates, by adjusting its rotating speed, can also play the effect of stirring, having the function of vortex instrument.
(3), due to the setting of magnetic post, magnetosheath is in rotary course, and magnetic bead remains on magnetosheath by the effect in magnetic field, and liquid is got rid of de-under the influence of centrifugal force, and magnetic bead is further separated with liquid.In addition, after being arranged to asperities to the outside surface of magnetosheath, it can play the effect of grinding in the process of rotating to mixed system, therefore have tissue, cytoclasis function.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1, Fig. 2 and Fig. 3 are the structural representation of multifunctional bio material disconnecting gear provided by the invention in " single head structure ";
Reference numeral:
Housing-101; Main shaft push rod-102; Magnetosheath shack-103; Electric device-104; Magnetic post-105; Magnetosheath-106; First collar-201; Second collar-202; First spring-203; Wedging position-204; Push rod parcel cover-205; Locating sleeve-206; Second spring-207; Magnetosheath departs from push rod-208; Press button-209; Handle-210; Follower gear-211;
Fig. 4, Fig. 5 are the structural representation of multifunctional bio material disconnecting gear in " bull structure " of confession of the present invention;
Reference numeral:
Housing-101; Main shaft push rod-102: magnetosheath shack-103; Electric device-104; Magnetosheath-106; Push rod parcel cover-205; Locating sleeve-206; Second spring-207; Magnetosheath departs from push rod-208; Press button-209; Handle-210; Follower gear-211; Bull link span-301; Follower lever-302; Union lever-303; Retaining plate-304; Protruding-305.
Embodiment
Please refer to Fig. 1-Fig. 3, this multifunctional bio material disconnecting gear provided by the invention, comprise housing 101, run through the main shaft push rod 102 of housing 101, returning device, magnetosheath shack 103 and electric device 104; The two ends of main shaft push rod 102 all protrude from housing 101, and its one end is provided with magnetic post 105 (embodying in fig. 2, lower same); Magnetosheath shack 103 (not shown in Fig. 1 and Fig. 3, corresponding position is embodied in fig. 2, lower same) is sheathed on main shaft push rod 102, and magnetosheath shack 103 one end be in outside housing 101 is provided with magnetosheath 106; It is inner that returning device is arranged on housing 101; And for making main shaft push rod 102 move to the direction away from magnetosheath 106; Electric device 104 is connected with magnetosheath shack 103, and for making magnetosheath shack 103 rotate.
Above-mentioned biological substance tripping device, it not only can be efficient, realizes the separating effect of mixed system (containing magnetic substance, as magnetic bead) rapidly; But also there is the effect of similar vortex instrument, instrument such as dehydration centrifuge, beveller etc., can be applied in biological experiment easily.
In comparatively preferred scheme, in housing 101, be also fixedly installed wedging position 204; Returning device comprises the first collar 201, second collar 202 and the first spring 203;
First collar 201, first spring 203 and second collar 202 are sheathed on main shaft push rod 102 respectively, first collar 201 offsets with wedging position 204, second collar 202 is fixedly connected with main shaft push rod 102, and the two ends of the first spring 203 offset with first collar 201 and second collar 202 respectively;
One end main shaft push rod 102 being in housing 101 outside is provided with push rod parcel cover 205; One end that push rod parcel cover 205 is closed is wrapped in the end of main shaft push rod 102, and open one end and second collar 202 offset.
For wedging position 204, its structure does not have particular determination, as long as be fixed on housing 101 inside and larger contact surface can be there is with first collar 201, in addition, preferably, in order to play the effect of fixed main shaft push rod 102, the free end of wedging position 204 is provided with a chute, this chute can pass for main shaft push rod 102, thus all plays locating effect to main shaft push rod 102, first collar 201, second collar 202 etc.In addition, for first collar 201 and second collar 202, be connected in order to realize between its with the first spring 203 firm, it includes the protuberance for sheathed spring (stretching into the two ends of spring).
The returning device with said structure fast and effeciently can realize the return effect of main shaft push rod 102; Can be forced in equably on main shaft push rod 102 by the first spring 203 compressed, thus impel its reposition.
In order to make main shaft push rod 102 steady, smooth and easy in reciprocating process further, on the basis of such scheme; Further, housing 101 inside is also fixedly installed locating sleeve 206, and locating sleeve 206 is sheathed on push rod parcel cover 205 and is in part in housing 101; The outer wall that the inwall of locating sleeve 206 and push rod wrap up cover 205 fits.
In the present invention, because magnetosheath 106 and magnetosheath shack 103 are sockets, therefore when magnetosheath 106 repeatedly uses or meet the demand operated, often need it to lift down from magnetosheath shack 103; And conveniently itself and magnetosheath shack 103 depart from, further preferably, on the basis of above-mentioned preferred version, also comprise and be arranged on the second spring 207 on housing 101 and magnetosheath departs from push rod 208; One end of second spring 207 is fixed on housing 101, and one end that the other end and magnetosheath depart from push rod 208 offsets, the opening part of the housing 101 that one end that the other end that magnetosheath departs from push rod 208 extends to the main shaft push rod 102 being equipped with magnetic post 105 is passed.
Magnetosheath departs from push rod 208 under the effect of external force, it moves along housing 101, be specially housing 101 end motion towards being provided with magnetosheath 106, it is in the process of motion, bottom can abut on magnetosheath 106, and then magnetosheath 106 can be departed from, after magnetosheath 106 departs from, under the effect of the second spring 207, magnetosheath departs from push rod 208 reposition.
Magnetosheath is departed to the structure of push rod 208, do not do concrete restriction, as long as it can meet along housing 101 to-and-fro movement, such as it can have channel-shaped structure, it is also fixedly installed a collar (embodying in figure 3), like this when being pushed from magnetosheath shack 103 by magnetosheath 106 equably collar during its other end applied thrust for the one end pushing away magnetosheath 106.For the second spring 207, its one end or part need to be fixed on housing 101, and the other end then departs from push rod 208 one end (two ends) with magnetosheath offsets.In addition, in order to make structure compact and reasonable more, be parallel between the first spring 203 and the second spring 207.
Further in preferred scheme, in housing 101, be also provided with circuit card (setting position is random, in the drawings and not shown); Circuit card is electrically connected with electric device 104, and circuit card is also provided with the motor adjusting knob and charge port (not shown in FIG.) that all protrude from housing 101; And the press button 209 housing 101 is also provided with for control circuit board power supply.
By circuit card, can integrated various circuit, motor adjusting knob can realize electric device 104 (being specially a motor) output rating and regulate, and directly controls the slewing rate of magnetosheath shack 103 and magnetosheath 106; Realize the speed adjustment of magnetosheath 106; And by charge port (for simplicity, can USB interface be arranged to), charging effect can be realized to power supply; In addition, housing 101 is also provided with the press button 209 for control circuit board power supply.In the process used, opening power press button 209, thus make electric device 104 enter working order, governor motor adjusting knob then can regulate the working efficiency of electric device 104.It is pointed out that the setting position for circuit card is random, and for motor adjusting knob, be then preferably arranged on the middle part of housing 101, make to use and be then convenient to governor motor power; Press button 209 is then arranged on the end of housing 101, is convenient to thumb press operation.
In addition, for the ease of taking, putting this biological substance tripping device, on the basis of above-mentioned preferred version, preferred further, housing 101 outer setting has a handle 210, and handle 210 one end is fixed on housing 101, and the other end bends to the direction towards magnetic post 105.
Generally, each biological substance tripping device configures a magnetosheath 106, i.e. " single head structure "; The appearance similar of this one-piece construction and conventional liquid-transfering gun, in order to make its magnetosheath 106 smooth rotation, on the basis of above-mentioned optimal technical scheme the most, it is inner that electric device 104 is arranged on housing 101, magnetosheath shack 103 is fixedly connected with a follower gear 211, electric device 104 is provided with a transmitting gear engaged with follower gear 211.
Incorporated by reference to Fig. 1-Fig. 3, in technique scheme, embody structure and the principle of work thereof of single head tripping device, but in the process of actually operating, when the sample of disposable process is more, the mode (namely each housing 101 configures a magnetosheath 106) of single head does not obviously meet batch processing demand, therefore, multiple magnetosheath 106 is preferably set, and realize controlling multiple magnetosheath 106 by a main shaft push rod 102 simultaneously, magnetic post 105 is very important.
Therefore, please refer to Fig. 4-Fig. 5, present invention also offers a kind of biological substance tripping device, it comprises: housing 101, the main shaft push rod 102 running through housing 101, returning device, magnetosheath shack 103 and electric device 104;
Main shaft push rod 102 (specifically comprises the transmission rod being in housing 101 inside and the follower lever 302 being in housing 101 outside in this scenario, wherein transmission rod is inner owing to being in housing 101, therefore do not illustrate in the drawings) two ends all protrude from housing 101, and its one end is provided with magnetic post 105; Magnetosheath shack 103 is sheathed on main shaft push rod 102 that (structure of magnetosheath shack 103 is consistent with shown in Fig. 1-Fig. 3, in this programme, magnetosheath shack 103 is specifically sheathed with on follower lever 302, in figures 4 and 5 owing to being stopped, therefore and not shown), and magnetosheath shack 103 one end be in outside housing 101 is provided with magnetosheath 106; It is inner that returning device is arranged on housing 101; And for making main shaft push rod 102 move to the direction away from magnetosheath 106; Electric device 104 is connected with magnetosheath shack 103, and for making magnetosheath shack 103 rotate;
Housing 101 inside is also fixedly installed locating sleeve 206, and locating sleeve 206 is sheathed on push rod parcel cover 205 and is in part in housing 101; The outer wall that the inwall of locating sleeve 206 and push rod wrap up cover 205 fits;
This device also comprises: be arranged on the second spring 207 on housing 101 and magnetosheath disengaging push rod 208; One end of second spring 207 is fixed on housing 101, and one end that the other end and magnetosheath depart from push rod 208 offsets, the opening part of the housing 101 that one end that the other end that magnetosheath departs from push rod 208 extends to the main shaft push rod 102 being equipped with magnetic post 105 is passed;
Also circuit card is provided with in housing 101; Circuit card is electrically connected with electric device 104, and circuit card is also provided with the motor adjusting knob and charge port that all protrude from housing 101; And the press button 209 housing 101 is also provided with for control circuit board power supply.
Housing 101 outer setting has a handle 210, and handle 210 one end is fixed on housing 101, and the other end bends to the direction towards magnetic post 105;
This biological substance tripping device also comprises a bull link span 301; Bull link span 301 is socketed with one end of housing 101; Main shaft push rod 102 comprises a transmission rod and Duo Gen follower lever 302; All follower levers 302 are arranged side by side, and are fixed by a union lever 303, and union lever 303 is fixedly connected with transmission rod; Transmission rod, follower lever 302 and union lever 303 are all arranged on bull link span 301;
All follower levers 302 are all through a retaining plate 304, and its one end through retaining plate 304 is provided with magnetic post 105, and retaining plate 304 is fixedly installed on bull link span 301;
Each follower lever 302 is all arranged with magnetosheath shack 103; On each magnetosheath shack 103, one end is provided with magnetosheath 106, and the other end is all fixed with gear; Intermesh between two often adjacent gears, electric device 104 is arranged on bull link span 301, and rotates for driving gear.
In the biological substance tripping device of above-mentioned " bull structure ", main shaft push rod 102 is divided into two parts, and the part be wherein in housing 101 is transmission rod; And housing 101 outer part draws together many follower levers 302 side by side; Many transmission rods are fixed by a union lever 303, and transmission rod is then fixedly connected with union lever 303.
After applying reactive force to push rod parcel cover 205, transmission rod then promotes union lever 303 and all follower levers 302 move, and 105, the magnetic post being now arranged on each follower lever 302 end is also pushed and stretches to the bottom of the magnetosheath 106 of its correspondence.In addition, due to each follower lever 302 being all arranged with magnetosheath shack 103; On each magnetosheath shack 103, one end is provided with magnetosheath 106, and the other end is all fixed with gear; Intermesh between two often adjacent gears, electric device 104 (being specially a motor) is arranged on bull link span 301 and (conveniently realizes multiple gear by an electric device 104 to rotate simultaneously).After electric device 104 runs, as long as drive in multiple gear gear to rotate can realize the rotation of all gear units, and then realize the rotation of all magnetosheaths 106 easily.
Due to structures such as bull link span 301 and retaining plates 304, (transmission rod is specially at main shaft push rod 102, be in housing 101 inner, at Fig. 5) when moving, be only all follower levers 302 (comprising the magnetic post 105 of its end), union lever 303 moves, and gear unit, retaining plate 304 and be geo-stationary between bull link span 301 and housing 101.
The above-mentioned biological substance tripping device with bull structure, corresponding construction and the single head biological substance tripping device of its housing 101 inside are basically identical, but multiple magnetosheaths 106 (comprising magnetic post 105) that the biological substance tripping device of this bull has due to it, it can carry out batch processing to the treatment solution arranged in advance simultaneously, thus improves processing efficiency.
Preferred further, transmission rod is all parallel with all follower levers 302, and union lever 303 is vertical with transmission rod; Magnetosheath 106 is one end open, the cavity that one end is closed, one end of its opening is set on magnetosheath shack 103, and the one end closed arranges multiple protruding 305, the structure of protruding 305 is specially fan blade, to increase the stirring of magnetosheath 106 in the process of rotating and vortex function.
This biological substance tripping device provided by the invention, it opens a novel approach for biological separating substances field; For its sharp separation DNA, step only has four steps, and the time completed at about one minute;
Concrete steps:
1. in the first pipe, add magnetic bead, lysate and peripheral blood, start rotary knob, accelerate to rotate with device, cell can be cleaved in moment, and namely DNA is discharged in lysate;
2. press the button that stops the rotation, promote main shaft push rod and provide magnetic field, magnetic bead, under the effect of magnetic post magnetic force, is inhaled in magnetosheath periphery;
3. the magnetic bead with DNA is cleaned in washing lotion;
4. DNA and magnetic bead are separated in elutriant comes, and then provide magnetic field, namely magnetic bead is picked up, and magnetic bead of throwing aside can obtain the DNA of purifying.
Visible, by this device, it simplifies the step that biological substance is separated greatly, sets up the milestone (extracting DNA in 1 minute) extracting DNA speed, creates the single instrument of biological substance separation field and complete multiple function precedent; Concrete, which create the novel method of dehydrating function in separate substance engineering; Set up the model that mini-instrument solves huge problem; Establish the new tool that in separating substances process, liquid mixing stirs; The ingenious combination (coming and going in the presence of a magnetic field and rotary motion) embodying the Design and Features of magnetosheath is worth; The function of grinding and disrupting tissue is increased after embodying magnetosheath alteration of form.
To sum up, multifunctional bio material disconnecting gear provided by the invention is magnetic-particle for removing fast all size and shape from liquid media and the one that designs is quick, light, quick, moveable new instrument.
This device is applicable to from contaminated liquids, remove pollutent or other impurity.Such as remove from blood, marrow, cerebrospinal fluid, cell culture medium, food, milk, beverage and purification virus, bacterium, toxin, hormone, medicine, sterilant, autoimmunization albumen, antigen, haptens etc.Also unwanted impurity can be removed from biological reagent, technical oils, chemical liquid.Sharp separation biological substance is mainly used in field of medical biology.Conventional has sharp separation nucleic acid, protein, virus and various cell from different types of animal or plant tissue; Also can be used for harmful virus in purged body (as hepatitis, acquired immune deficiency syndrome (AIDS)), cancer cells (as leukemia), autoimmunization albumen (as systemic lupus erythematous, rheumatoid arthritis) etc. and play the object for the treatment of and alleviating disease.
This multifunctional bio material disconnecting gear, in the biological substance separation of medical science, field of biology, is that its velocity of separation, tolerance range, agility have all surmounted current Routine Test Lab overwhelming majority instrument.Convenient, the operation of dexterous, the assembly of function of its body, application simple, set up single type in world's like product and complete the milestone of several functions; Multifunctional bio material disconnecting gear environmental protection and energy saving, safe and harmless in and all create the first in the world of like product in the speed to be separated at biological substance and precision.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. a multifunctional bio material disconnecting gear, is characterized in that, comprises housing, runs through the main shaft push rod of described housing, returning device, magnetosheath shack and electric device;
The two ends of described main shaft push rod all protrude from described housing, and its one end is provided with magnetic post; Described magnetosheath shack is sheathed on described main shaft push rod, and one end that described magnetosheath shack is in outside described housing is provided with magnetosheath;
Described returning device is arranged on described enclosure interior; And for making described main shaft push rod move to the direction away from described magnetosheath; Described electric device is connected with described magnetosheath shack, and for making described magnetosheath shack rotate.
2. multifunctional bio material disconnecting gear according to claim 1, is characterized in that, is also fixedly installed wedging position in described housing; Described returning device comprises first collar, second collar and the first spring;
Described first collar, the first spring and second collar are sheathed on described main shaft push rod respectively, described first collar and described wedging position offset, described second collar is fixedly connected with described main shaft push rod, and the two ends of described first spring offset with described first collar and described second collar respectively;
The one end described main shaft push rod being in described hull outside is provided with push rod parcel cover; One end that described push rod parcel cover is closed is wrapped in the end of described main shaft push rod, and open one end and described second collar offset.
3. multifunctional bio material disconnecting gear according to claim 2, is characterized in that, described enclosure interior is also fixedly installed locating sleeve, and described locating sleeve is sheathed on described push rod parcel and overlaps the part be in described housing; The inwall of described locating sleeve and described push rod wrap up the outer wall overlapped and fit.
4. multifunctional bio material disconnecting gear according to claim 3, is characterized in that, also comprises setting the second spring on the housing and magnetosheath departs from push rod; One end of described second spring is fixing, and one end that the other end and described magnetosheath depart from push rod offsets on the housing, and the other end of described magnetosheath disengaging push rod extends to the opening part of the described housing passed by magnetic post.
5. multifunctional bio material disconnecting gear according to claim 4, is characterized in that, is also provided with circuit card in described housing; Described circuit card is electrically connected with described electric device, and described circuit card is also provided with the motor adjusting knob and charge port that all protrude from described housing; And the press button described housing is also provided with for control circuit board power supply.
6. multifunctional bio material disconnecting gear according to claim 5, is characterized in that, described hull outside is provided with a handle, and described handle one end is fixing on the housing, and the other end bends to the direction towards magnetic post.
7. multifunctional bio material disconnecting gear according to claim 6, it is characterized in that, described electric device is arranged on described enclosure interior, described magnetosheath shack is fixedly connected with a follower gear, described electric device is provided with a transmitting gear engaged with described follower gear.
8. multifunctional bio material disconnecting gear according to claim 6, is characterized in that, described biological substance tripping device also comprises a bull link span; One end of described bull link span and described housing is socketed;
Described main shaft push rod comprises a transmission rod and Duo Gen follower lever; All follower levers are arranged side by side, and are fixed by a union lever, and described union lever is fixedly connected with described transmission rod; Described transmission rod, follower lever and union lever are all arranged on described bull link span;
All described follower levers are all through a retaining plate, and its one end through described retaining plate is provided with magnetic post, and described retaining plate is fixedly installed on described bull link span;
Each described follower lever is all arranged with magnetosheath shack; One end of each magnetosheath shack is provided with magnetosheath, and the other end is all fixed with follower gear; Intermesh between two often adjacent follower gears, described electric device is arranged on described bull link span, and for driving described follower gear to rotate.
9. multifunctional bio material disconnecting gear according to claim 8, is characterized in that, described transmission rod is all parallel with all described follower levers, and described union lever is vertical with described transmission rod.
10. the multifunctional bio material disconnecting gear according to any one of claim 1-9, is characterized in that, described magnetosheath is one end open, the cavity that one end is closed, and one end of its opening is set on described magnetosheath shack, and the one end closed arranges multiple projection.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610034026.7A CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
| PCT/CA2016/000204 WO2017139864A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| US16/071,038 US20190022667A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| EP16890137.9A EP3405288A4 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
| CA3008660A CA3008660A1 (en) | 2016-01-19 | 2016-08-05 | Multifunctional biological substance separation device |
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| CN201610034026.7A CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
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| CN105420074A true CN105420074A (en) | 2016-03-23 |
| CN105420074B CN105420074B (en) | 2018-06-29 |
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| CN201610034026.7A Expired - Fee Related CN105420074B (en) | 2016-01-19 | 2016-01-19 | A kind of multifunctional bio material disconnecting gear |
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Effective date of registration: 20170216 Address after: 030600 Jinzhong Development Zone, Shanxi Hui Road, No. 8 Applicant after: SHANXI ZDGSY BIO-SCIENTIFIC CO., LTD. Applicant after: Shanxi Traditional Chinese Medical College Address before: 030600 Jinzhong Economic Development Zone, Shanxi Road No. 8 Applicant before: SHANXI ZDGSY BIO-SCIENTIFIC CO., LTD. |
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