CN205635636U - A magnetic beads sorting device for nucleic acid extraction - Google Patents

Abstract

The utility model provides a magnetic beads sorting device for nucleic acid extraction, break away from module, separation sleeve and reaction consumptive material including release head lifting module, bar magnet module, stirring module, separation sleeve, bar magnet module and stirring module respectively with release head lifting module sliding connection, but the bar magnet module includes the bar magnet of a plurality of up -and -down motions, stirring module include a plurality ofly and the corresponding gear shaft of bar magnet, through gear connection between the adjacent gear shaft, the core cavity of gear shaft, the gear shaft lower part is used for cup jointing the separation sleeve, the separation sleeve breaks away from the module to be fixed on stirring module, and the separation sleeve breaks away from the module and is used for taking off the separation sleeve from the gear shaft, and the reaction consumptive material sets up in the below of gear shaft, and the reaction consumptive material includes cracking container, washing container and elution container. The utility model discloses a bar magnet is permanent magnet, and permanent magnet's structure is smaller and more exquisite. The utility model discloses a liquid mixing mode is rotatory stirring mixing, and the rotation rate can be transferred, and the mixing uniformity is good.

Description

A kind of magnetic bead separating device for nucleic acid extraction

Technical field

This utility model relates to biological technical field, more particularly, to a kind of magnetic bead separating device for nucleic acid extraction.

Background technology

Nucleic acid includes two kinds of molecules of DNA, RNA, biotechnology with nucleic acid as object of study, including to the extraction of nucleic acid, clone, expand, detect, a series of technology such as order-checking, develop rapidly in recent years, these technology the most not only use in research unit, and the most multiple functional department the most extensively applies.And the first step of nucleic acids research, it is simply that extracting nucleic acid from various biological samples, the efficiency of nucleic acid extraction becomes the restraining factors of downstream nucleic acid research success.Nucleic acid is all to exist with the state with protein bound in cell, mainly comprising the following steps of nucleic acid extraction: the impurity such as cell lysis removes the protein and the biomacromolecule such as polysaccharide, lipid being combined with nucleic acid, and purification of nucleic acid, removes salt, organic agent.

Existing nucleic acid extraction substantially uses paramagnetic particle method, uses nanotechnology to improve the surface of superparamagnetic nano particle and after the modification of surface, is prepared as superparamagnetism silicon oxide nanometer magnetic bead.This magnetic bead can specifically identify with nucleic acid molecules on micro interface and efficiently be combined.Utilize the superparamagnetism of silicon oxide Nano microsphere, under the effect of Chaotropic salt (guanidine hydrochloride, guanidinium isothiocyanate etc.) and externally-applied magnetic field, can separate by DNA and RNA from blood, animal tissue, food, pathogenic microorganism equal samples, can be applicable to clinical disease diagnosis, transfusion safety, Forensic Identification, disease control detection, nucleic acid samples build the multiple fields such as storehouse, environmental microorganism detection, food safety detection, molecular biology research.

Common manual nucleic acid extraction uses vibrator to mix, and common automatic nucleic acid-extracting apparatus uses the mode of up-down vibration, and up-down vibration mixing concordance is poorer than Stirring mixing.And, existing nucleic acid-extracting apparatus generally employing electric magnet adsorbs and shifts magnetic bead, and electric magnet needs to connect circuit, and the volume of magnet spool is the hugest.

Utility model content

This utility model is to overcome at least one defect described in above-mentioned prior art, it is provided that a kind of compact structure, the magnetic bead separating device for nucleic acid extraction that mixing concordance is good.

For solving above-mentioned technical problem, the technical solution of the utility model is as follows:

A kind of magnetic bead separating device for nucleic acid extraction, described device includes that separating head lifts module, bar magnet module, stirring module, separation sleeve departs from module, separation sleeve and reaction consumptive material, described bar magnet module and stirring module are slidably connected with separating head lifting module respectively, described bar magnet module includes some bar magnets moved up and down, described stirring module includes the gear shaft that some and described bar magnet is corresponding, connected by gear between adjacent teeth wheel shaft, the core hollow of gear shaft, gear shaft bottom is used for being socketed separation sleeve, described separation sleeve departs from module and is fixed in stirring module, separation sleeve departs from module for being taken off from gear shaft by separation sleeve, described reaction consumptive material is arranged at the lower section of gear shaft, reaction consumptive material includes cracking container, washing container and elution container.

In the preferred scheme of one, described separating head lifting module includes the first base and the first linear electric motors being arranged on the first base and the first line slideway, first line slideway is provided with slide, first linear electric motors are connected with slide by the first screw mandrel, and the first linear electric motors drive slide to move along a straight line along the first line slideway by the first screw mandrel.

In the preferred scheme of one, described bar magnet module includes the second base and the second linear electric motors being arranged on the second base and the second line slideway, second base is fixing with the slide of separating head lifting module to be connected, second line slideway is provided with bar magnet seat, second linear electric motors are connected with bar magnet seat by the second screw mandrel, being fixed with some bar magnets on bar magnet seat, the second linear electric motors drive the bar magnet on bar magnet seat to move along a straight line along the second line slideway by the second screw mandrel.

In the preferred scheme of one, described stirring module includes the 3rd base and the electric rotating machine being arranged on the 3rd base gear shaft corresponding with some and described bar magnet, 3rd base is fixing with the slide of separating head lifting module to be connected, gear shaft is fixed on the 3rd base by bearing, connect to realize synchronous rotary by gear between adjacent gear shaft, the motor shaft of electric rotating machine is connected with gear shaft by drivewheel, the core hollow of gear shaft, gear shaft bottom is used for being socketed separation sleeve, electric rotating machine does the rotary motion synchronized by drivewheel gear shaft and separation sleeve；Separation sleeve is disposable consumptive material, can be taken off or reinserts at any time with gear shaft, bar magnet isolation, separation sleeve by liquid, play isolation, protection gear shaft and the effect of bar magnet.

In the preferred scheme of one, described separation sleeve departs from module and includes motor mounting plate and the 3rd linear electric motors being arranged on motor mounting plate and the 3rd line slideway, motor mounting plate is fixing with the 3rd base of stirring module to be connected, 3rd line slideway is provided with de-block, 3rd linear electric motors are connected with de-block by the 3rd screw mandrel, de-block is provided with axis hole the most some with described gear shaft, and gear shaft correspondence is inserted into each axis hole, and the internal diameter of axis hole is less than the external diameter of the upper surface of separation sleeve；3rd linear electric motors drive de-block to move along a straight line along the 3rd line slideway by the 3rd screw mandrel, promote the upper surface of separation sleeve, thus returned from gear shaft by separation sleeve when de-block moves downward.

In the preferred scheme of one, described bar magnet is permanent-magnet magnetic iron staff.

In the preferred scheme of one, some gear shafts arrange in the way of multiple rows of multiple row or single multiple row.

In the preferred scheme of one; described separation sleeve is disposable consumptive material, and it is socketed in gear shaft bottom, by liquid and gear shaft, bar magnet isolation; separation sleeve can be taken off or reinsert, and plays isolation, is protected from polluting gear shaft, the effect done of bar magnet.

In the preferred scheme of one, if described reaction consumptive material includes and described gear shaft stem cracking container one to one, some washing containers and some elution container.

In the preferred scheme of one, described reaction consumptive material also includes separation sleeve container for hold separation sleeve the most some with described gear shaft.

The operation principle of this utility model device is: under blood sample is catalyzed by reagent such as lysates, cracks out nucleic acid and other impurity, and under the effect combining liquid, the superparamagnetism silicon oxide nanometer magnetic bead absorption nucleic acid in the mixed liquor of reaction consumptive material combines together.Separation sleeve is inserted into bar magnet, bar magnet end face closely cooperates with separation sleeve point, the separation sleeve being inserted with bar magnet moves to react bottom consumptive material from liquid level, under the magneticaction that bar magnet produces, the superparamagnetism silicon oxide nanometer magnetic bead being adsorbed with nucleic acid is magnetized, and adsorb on the set point of separation sleeve, separated with separation sleeve with bar magnet by separating head lifting, can be transported to the magnetic bead being adsorbed with nucleic acid react in other container of consumptive material.In handling process, gap between magnetic bead surfaces, magnetic bead and magnetic bead can remain other impurity such as a small amount of lysate, such as protein, all impurity are washed off by washing below exactly, under the effect of eluent, clean nucleic acid and Beads enrichment, siphon away magnetic bead, clean nucleic acid and the mixed liquor of eluent are final extraction product, and the function of this device completes cracking, washing, eluting exactly, obtains final nucleic acid extraction product.

Compared with prior art, technical solutions of the utility model provide the benefit that:

1), bar magnet be permanent magnet, the structure comparison of permanent magnet is small and exquisite.Prior art commonly inserts magnetizable iron staff in the middle of electric magnet, coil, and by coil electricity produces magnetic field magnetization iron staff, the volume of coil is the hugest.

2), blending manner be Stirring mixing, rotary speed can adjust, and mixing concordance is good.Common manual nucleic acid extraction uses vibrator to mix, and common automatic nucleic acid extraction uses the mode of up-down vibration, and up-down vibration mixing concordance is poorer than Stirring mixing.

Accompanying drawing explanation

Fig. 1 is the structural representation of this utility model magnetic bead separating device.

Fig. 2 is the structural blast figure of this utility model magnetic bead separating device.

Fig. 3 is the structural representation of separating head lifting module.

Fig. 4 is the structural representation of bar magnet module.

Fig. 5 is the structural representation of stirring module.

Fig. 6 is the structural representation that separation sleeve departs from module.

Fig. 7 is the top view that gear shaft arranges with multiple rows of multi-columnar.

Fig. 8 is the top view that gear shaft arranges with single multi-columnar.

Fig. 9 is the structural representation of reaction consumptive material.

Figure 10 is the top view of reaction consumptive material.

Figure 11 is the schematic diagram that separation sleeve container moves to the lower section of gear shaft.

Figure 12 is the schematic diagram that gear shaft inserts separation sleeve.

Figure 13 is the schematic diagram that separation sleeve is taken up by gear shaft.

Figure 14 is the schematic diagram of the laminating bottom bar magnet and separation sleeve.

Figure 15 is the schematic diagram that magnetic bead is adsorbed onto separation sleeve set point.

Figure 16 is to take the magnetic bead of separation sleeve set point to schematic diagram outside cracking container.

Figure 17 is the schematic diagram that separation sleeve separates with gear shaft.

Wherein: 1, separating head lifting module；2, bar magnet module；3, stirring module；4, separation sleeve departs from module；5, separation sleeve；6, reaction consumptive material.

101, the first base；102, the first linear electric motors；103, the first line slideway；104, slide；105, the first screw mandrel.

201, the second base；202, the second linear electric motors；203, the second line slideway；204, bar magnet seat；205, the second screw mandrel；206, bar magnet.

301, the 3rd base；302, electric rotating machine；303, gear shaft；304, drivewheel.

401, motor mounting plate；402, the 3rd linear electric motors；403, the 3rd line slideway；404, de-block；405, the 3rd screw mandrel；406, axis hole.

601, cracking container；602, washing container；603, elution container；604, separation sleeve container.

Detailed description of the invention

Accompanying drawing being merely cited for property explanation, it is impossible to be interpreted as the restriction to this patent；

In order to the present embodiment is more preferably described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product；

To those skilled in the art, in accompanying drawing, some known features and explanation thereof may will be understood by omission.

With embodiment, the technical solution of the utility model is described further below in conjunction with the accompanying drawings.

Embodiment 1

As shown in figures 1 to 6, the present embodiment provides a kind of magnetic bead separating device for nucleic acid extraction, and it includes that separating head lifting module 1, bar magnet module 2, stirring module 3, separation sleeve depart from module 4, separation sleeve 5 and reaction consumptive material 6, and each module is specific as follows:

Separating head lifting module 1: include the first base 101 and the first linear electric motors 102 and the first line slideway 103 being arranged on the first base 101, first line slideway 103 is provided with slide 104, first linear electric motors 102 are connected with slide 104 by the first screw mandrel 105, and the first linear electric motors 102 drive slide 104 to move along a straight line along the first line slideway 103 by the first screw mandrel 105.

Bar magnet module 2: include the second base 201 and the second linear electric motors 202 and the second line slideway 203 being arranged on the second base 201, second base 201 is fixing with the slide 104 of separating head lifting module 1 to be connected, second line slideway 203 is provided with bar magnet seat 204, second linear electric motors 202 are connected with bar magnet seat 204 by the second screw mandrel 205, being fixed with some bar magnets 206 on bar magnet seat 204, the second linear electric motors 202 drive the bar magnet 206 on bar magnet seat 204 to move along a straight line along the second line slideway 203 by the second screw mandrel 205.Described bar magnet 206 is permanent-magnet magnetic iron staff.

Stirring module 3: include the 3rd base 301 and be arranged on the electric rotating machine 302 on the 3rd base 301 and the corresponding gear shaft 303 of some and described bar magnet 206, 3rd base 301 is fixing with the slide 104 of separating head lifting module 1 to be connected, gear shaft 303 is fixed on the 3rd base 301 by bearing, connect to realize synchronous rotary by gear between adjacent gear shaft 303, the motor shaft of electric rotating machine 302 is connected with gear shaft 303 by drivewheel 304, the core hollow of gear shaft 303, gear shaft 303 bottom is used for being socketed separation sleeve 5, electric rotating machine 302 does, by drivewheel 304 gear shaft 303 and separation sleeve 5, the rotary motion synchronized.

In the present embodiment, gear spindle drive mode uses intermeshing gear drive, but the kind of drive is not limited to gear, it is also possible to using friction pulley, belt, toothed belt transmission, the above kind of drive is all within protection domain.

In the present embodiment, as Figure 7-8, some bar magnets and some gear shafts arrange in the way of multiple rows of multiple row or single multiple row.In the present embodiment, the mode of the 6x4=24 part that bar magnet 206 and gear shaft 303 use, other arrangement modes are the most all within this utility model protection domain, and its number can be from 1 to without pieces.

Separation sleeve departs from module 4: include motor mounting plate 401 and the 3rd linear electric motors 402 and the 3rd line slideway 403 being arranged on motor mounting plate 401, motor mounting plate 401 is fixing with the 3rd base 301 of stirring module 3 to be connected, 3rd line slideway 403 is provided with de-block 404,3rd linear electric motors 402 are connected with de-block 404 by the 3rd screw mandrel 405, de-block 404 is provided with and the described the most some axis holes of gear shaft 303 406, gear shaft 303 correspondence is inserted into each axis hole 406, and the internal diameter of axis hole 406 is less than the external diameter of the upper surface of separation sleeve 5；3rd linear electric motors 402 drive de-block 404 to move along a straight line along the 3rd line slideway 403 by the 3rd screw mandrel 405, and de-block 404 promotes the upper surface of separation sleeve 5 when moving downward, thus is returned from gear shaft 303 by separation sleeve 5.

It should be noted that this utility model separation sleeve departs from the more than one of the above of structure of module 4, as long as separation sleeve 5 can be taken off from gear shaft 303 by the de-block 404 that separation sleeve departs from module 4.Such as 4 holes of same row or 6 hole millings of de-block 404 being worn and make channel-shaped, when needing de-separation sleeve 5, de-block 404 moves horizontally above insertion separation sleeve 5, when gear shaft 303 is raised, de-block 404 remains stationary as, and the most and then raises, de-block 404 will block separation sleeve 5, and separation sleeve 5 also can be taken off.

Separation sleeve 5: separation sleeve 5 is disposable consumptive material; for being socketed in gear shaft 303 bottom; being isolated with gear shaft 303, bar magnet 206 by liquid, separation sleeve can be taken off or reinsert at any time, plays isolation, is protected from polluting gear shaft, the effect done of bar magnet.

Reaction consumptive material 6: if including and described gear shaft stem cracking container 601 one to one, some washing containers 602, some elution container 603 and some separation sleeve containers 604, separation sleeve container 604 is used for holding separation sleeve 5.In the present embodiment, reaction consumptive material is as shown in figs. 9-10.

The present embodiment is for the magnetic bead separating device of nucleic acid extraction, and its workflow is as follows:

4.1, the separation sleeve container 604 that will be equipped with separation sleeve 5 moves to the lower section of device middle gear axle 303, as shown in figure 11.

4.2, the first linear electric motors 102 of separating head lifting module 1 work, and slide 104 is moved to bottom, allows gear shaft 303 insert in separation sleeve 5, as shown in figure 12.

4.3, the first linear electric motors 102 reverse operation, moves to the top by slide 104, and separation sleeve 5 is taken up by gear shaft 303, and separation sleeve 5 is socketed in gear shaft 303 bottom, as shown in figure 13.

4.4, by various blood samples, lysate, combine the various reagent such as liquid be sequentially added into cracking container 601 in, cracking container 601 is placed in below gear shaft 303.

4.5, the first linear electric motors 102 of separating head lifting module 1 work, and slide 104 moves to lower section, allow separation sleeve 5 insert in cracking container 601.

4.6, the electric rotating machine 302 of stirring module 3 works, and gear shaft 303 carries out Stirring with separation sleeve 5, and solution is sufficiently stirred for mixing, under the catalysis of various reagent, blood sample has fully cracked reaction, and nucleic acid, protein, inorganic salt etc. crack out from blood sample, are present in mixed liquor.

4.7, the first linear electric motors 102 reverse operation, moves to the top by slide 104, and separation sleeve 5 is taken up by gear shaft 303.

4.8, magnetic bead and other reagent are sequentially added in cracking container 601.

4.9, the first linear electric motors 102 of separating head lifting module 1 work, and slide 104 moves to lower section, allow separation sleeve 5 insert in cracking container 601.

4.10, the electric rotating machine 302 of stirring module 3 works, gear shaft 303 carries out Stirring with separation sleeve 5, solution is sufficiently stirred for mixing, under the effect combining liquid, cracking nucleic acid out can adsorb on magnetic bead, if magnetic bead amount is enough, free nucleic acid in the solution can all adsorb on magnetic bead, combines together.

4.11, the first linear electric motors 102 reverse operation, moves to the top by slide 104, and separation sleeve 5 is taken up by gear shaft 303.

4.12, the second linear electric motors 202 of bar magnet module 2 work, and bar magnet seat 204 and bar magnet 206 are driven into lower section, the bar magnet 206 that permanent magnet makes through gear shaft 303 core and with separation sleeve 5 bottom laminating, as shown in figure 14.

4.13, the first linear electric motors 102 work, slide 104 is included, and bar magnet module 2 whole parts such as grade move to crack the mixed liquor ullage in container 601, the bottom moving to cracking container 601 from liquid level the most at the uniform velocity, by position sharp for the set of the most or all of magnetic bead being adsorbed with nucleic acid in cracking container 601 and a small amount of lysate, impurity absorption to separation sleeve 5, as shown in figure 15.

4.14, the first linear electric motors 102 reverse operation, slide 104 is included, and bar magnet module 2 whole parts such as grade move to the top, because bar magnet 206 and separation sleeve 5 do not have relative motion, concentrate on separation sleeve 5 to overlap the magnetic bead being adsorbed with nucleic acid of point and be brought to crack outside container 601, magnetic bead and nucleic acid in mixed liquor the most not or remain few, as shown in figure 16.

4.15, being taken away by original cracking container 601, washing container 602 is placed on the position of primary fissure solution container 601, cleaning mixture is put into washing container 602, figure is with 4.14.

Illustrate: present case is changed the mode of differential responses consumptive material and described, this utility model is not limited to change reaction consumptive material 6, cracking container 601, washing container 602 and elution container 603 can also be integrated with on same reaction consumptive material 6, step before 4.15 is a round therein, 4.15 start be reaction the second round of consumptive material, the 3rd round etc..

4.16, the first linear electric motors 102 work, and slide 104 including, bar magnet module 2 whole part such as grade moves the bottom of washing container 602, and figure is with 4.13.

4.17, the second linear electric motors 202 of bar magnet module 2 work, and bar magnet seat 204 and bar magnet 206 are driven into the top, and remaining remains stationary as, allow bar magnet 206 and separation sleeve 5 away from, the magnetic bead that separation sleeve 5 is adsorbed with nucleic acid is dissolved in the cleaning mixture in washing container 602.

4.18, the electric rotating machine 302 of stirring module 3 works, gear shaft 303 carries out Stirring with separation sleeve 5, being sufficiently stirred for mixing by being adsorbed with the impurity such as the magnetic bead of nucleic acid, the lysate of residual, protein and cleaning mixture, do not overlap on point being attached to separation sleeve 5, this process is washing.

4.19, the first linear electric motors 102 reverse operation, moves to the top by slide 104, and separation sleeve 5 is taken up by gear shaft 303.

4.20, the second linear electric motors 202 of bar magnet module 2 work, and bar magnet seat 204 and bar magnet 206 are driven into lower section, and bar magnet 206 through the core of gear shaft 303 and is fitted with the external member bottom separation sleeve 5.

4.21, the first linear electric motors 102 work, slide 104 is included the mixed liquor ullage that bar magnet module 2 whole parts such as grade move in washing container 602, the bottom moving to washing container 602 from liquid level the most at the uniform velocity, is adsorbed onto separation sleeve 5 by the magnetic bead being adsorbed with nucleic acid of the overwhelming majority in washing container 602 and overlaps the position of point.

4.22, the first linear electric motors 102 reverse operation, slide 104 is included, and bar magnet module 2 whole parts such as grade move to the top, because bar magnet 206 and separation sleeve 5 do not have relative motion, concentrate on separation sleeve 5 overlap point the magnetic bead being adsorbed with nucleic acid be brought to outside washing container 602, now, on magnetic bead residual wash lysate, impurity has been cleaned or has been remained seldom.

4.23, the washing process of repetition 4.15-4.22 1 ~ 3 time, thoroughly washes away the impurity and lysate remaining on magnetic bead.

4.24, washing container 602 is taken away, elution container 603 is placed on the position of former washing container 602, eluent is loaded in elution container 603.

4.25, the first linear electric motors 102 work, and slide 104 including, bar magnet module 2 and stirring module 3 move down, and gear shaft 303 drives separation sleeve 5 to extend in elution container 603.

4.26, the second linear electric motors 202 of bar magnet module 2 work, and bar magnet seat 204 and bar magnet 206 are driven into the top, and remaining remains stationary as, allow bar magnet 206 and separation sleeve 5 away from, the magnetic bead that separation sleeve 5 is adsorbed with nucleic acid is dissolved in eluent.

4.27, the electric rotating machine 302 of stirring module 3 works, and gear shaft 303 carries out Stirring with separation sleeve 5, and with eluent, the magnetic bead being adsorbed with nucleic acid is sufficiently stirred for mixing, under the effect of eluent, nucleic acid will and Beads enrichment, nucleic acid is all dissolved in eluent, and this process is elution process.

4.28, the first linear electric motors 102 reverse operation, moves to the top by slide 104, and separation sleeve 5 is taken up by gear shaft 303.

4.29, the second linear electric motors 202 of bar magnet module 2 work, and bar magnet seat 204 and bar magnet 206 are driven into lower section, and bar magnet 206 is fitted with separation sleeve 5.

4.30, the first linear electric motors 102 work, slide 104 is included the mixed liquor ullage that bar magnet module 2 and stirring module 3 move in elution container 603, the separation sleeve 5 that makes the most at the uniform velocity moves to the bottom of elution container 603 from liquid level, and the magnetic bead of the overwhelming majority in elution container 603 concentrates on the position of the set point of separation sleeve 5.

4.31, the first linear electric motors 102 reverse operation, slide 104 is included, and bar magnet module 2 and stirring module 3 move to the top, because bar magnet 206 and separation sleeve 5 do not have relative motion, concentrate on separation sleeve 5 overlap point magnetic bead be all brought to outside elution container 603, the magnetic bead that nucleic acid in elution container 603 and the mixed liquor of eluent are contained within is the most not or residual is few, and the mixed liquor of nucleic acid and eluent is the extraction end product extracted.

4.32, elution container 603 is taken away, i.e. the end product extracted is taken away, for subsequent handling.

4.33, empty separation sleeve container 604 is placed on below gear shaft 303.

4.34, the 3rd linear electric motors 402 of separation sleeve disengaging module 4 work, de-block 404 is driven to move downward, under de-block 404 promotes, separation sleeve 5 separates with gear shaft, separation sleeve 5 is fallen back in the separation sleeve container 604 below gear shaft 303, de-block 404 resets, and takes the separation sleeve container 604 equipped with separation sleeve away and i.e. completes whole workflow, as shown in figure 17.

In sum, the process of 4.1 ~ 4.34 is exactly: take separation sleeve 5, by blood sample, lysate, combine liquid put into cracking container 601, Stirring mixes, and is sufficiently complete cracking, and nucleic acid, protein, inorganic salt etc. are separated and be mixed in mixed liquor, add magnetic bead, it is fully rotatable stirring and evenly mixing, allows nucleic acid absorption on magnetic bead, allow nucleic acid absorption combine together on magnetic bead under the effect combining liquid.Separation sleeve 5 core inserts bar magnet 206, the magnetic bead of absorption nucleic acid is picked up, cracking container 601 is replaced by washing container 602, bar magnet 206 separates with separation sleeve 5, and Stirring mixes, and allows being adsorbed with the magnetic bead of nucleic acid and impurity is thoroughly mixed in cleaning mixture, separation sleeve 5 inserts bar magnet 206, being picked up from cleaning mixture by the magnetic bead being adsorbed with nucleic acid, impurity is stayed in cleaning mixture, adsorbs the impurity contained at the nucleic acid of magnetic bead and tails off.Repeat above-mentioned washing process 1-3 time, adsorb the impurity that the nucleic acid at magnetic bead contains and reduce further.Washing container 602 is replaced by elution container 603 again, adds eluent, be fully rotatable stirring and evenly mixing, under the effect of eluent, magnetic bead and separate nucleic acid, separation sleeve 5 inserts bar magnet and siphons away magnetic bead, and the mixed liquor being eluted out nucleic acid and eluent in elution container 603 is final extraction product.Separation sleeve 5 is returned from gear shaft 303, completes workflow.

The corresponding same or analogous parts of same or analogous label；

Term the being merely cited for property explanation of position relationship described in accompanying drawing, it is impossible to be interpreted as the restriction to this patent；

Obviously, above-described embodiment of the present utility model is only for clearly demonstrating this utility model example, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.All any amendment, equivalent and improvement etc. made within spirit of the present utility model and principle, within should be included in this utility model scope of the claims.

Claims (10)

1. the magnetic bead separating device for nucleic acid extraction, it is characterized in that, described device includes that separating head lifts module, bar magnet module, stirring module, separation sleeve departs from module, separation sleeve and reaction consumptive material, described bar magnet module and stirring module are slidably connected with separating head lifting module respectively, described bar magnet module includes some bar magnets moved up and down, described stirring module includes the gear shaft that some and described bar magnet is corresponding, connected by gear between adjacent teeth wheel shaft, the core hollow of gear shaft, gear shaft bottom is used for being socketed separation sleeve, described separation sleeve departs from module and is fixed in stirring module, separation sleeve departs from module for being taken off from gear shaft by separation sleeve, described reaction consumptive material is arranged at the lower section of gear shaft, reaction consumptive material includes cracking container, washing container and elution container.

Magnetic bead separating device for nucleic acid extraction the most according to claim 1, it is characterized in that, described separating head lifting module includes the first base and the first linear electric motors being arranged on the first base and the first line slideway, first line slideway is provided with slide, first linear electric motors are connected with slide by the first screw mandrel, and the first linear electric motors drive slide to move along a straight line along the first line slideway by the first screw mandrel.

Magnetic bead separating device for nucleic acid extraction the most according to claim 2, it is characterized in that, described bar magnet module includes the second base and the second linear electric motors being arranged on the second base and the second line slideway, second base is fixing with the slide of separating head lifting module to be connected, second line slideway is provided with bar magnet seat, second linear electric motors are connected with bar magnet seat by the second screw mandrel, being fixed with some bar magnets on bar magnet seat, the second linear electric motors drive the bar magnet on bar magnet seat to move along a straight line along the second line slideway by the second screw mandrel.

Magnetic bead separating device for nucleic acid extraction the most according to claim 3, it is characterized in that, described stirring module includes the 3rd base and the electric rotating machine being arranged on the 3rd base gear shaft corresponding with some and described bar magnet, 3rd base is fixing with the slide of separating head lifting module to be connected, gear shaft is fixed on the 3rd base by bearing, connect to realize synchronous rotary by gear between adjacent gear shaft, the motor shaft of electric rotating machine is connected with gear shaft by drivewheel, the core hollow of gear shaft, gear shaft bottom is used for being socketed separation sleeve, electric rotating machine does the rotary motion synchronized by drivewheel gear shaft and separation sleeve；Separation sleeve is disposable consumptive material, can be taken off or reinserts at any time with gear shaft, bar magnet isolation, separation sleeve by liquid, play isolation, protection gear shaft and the effect of bar magnet.

Magnetic bead separating device for nucleic acid extraction the most according to claim 4, it is characterized in that, described separation sleeve departs from module and includes motor mounting plate and the 3rd linear electric motors being arranged on motor mounting plate and the 3rd line slideway, motor mounting plate is fixing with the 3rd base of stirring module to be connected, 3rd line slideway is provided with de-block, 3rd linear electric motors are connected with de-block by the 3rd screw mandrel, de-block is provided with axis hole the most some with described gear shaft, gear shaft correspondence is inserted into each axis hole, and the internal diameter of axis hole is less than the external diameter of the upper surface of separation sleeve；3rd linear electric motors drive de-block to move along a straight line along the 3rd line slideway by the 3rd screw mandrel, promote the upper surface of separation sleeve, thus returned from gear shaft by separation sleeve when de-block moves downward.

Magnetic bead separating device for nucleic acid extraction the most according to claim 1, it is characterised in that described bar magnet is permanent-magnet magnetic iron staff.

Magnetic bead separating device for nucleic acid extraction the most according to claim 1, it is characterised in that some gear shafts arrange in the way of multiple rows of multiple row or single multiple row.

Magnetic bead separating device for nucleic acid extraction the most according to claim 1; it is characterized in that; described separation sleeve is disposable consumptive material; it is socketed in gear shaft bottom; by liquid and gear shaft, bar magnet isolation; separation sleeve can be taken off or reinsert, and plays isolation, is protected from polluting gear shaft, the effect done of bar magnet.

Magnetic bead separating device for nucleic acid extraction the most according to claim 1, it is characterised in that if described reaction consumptive material includes and described gear shaft stem cracking container one to one, some washing containers and some elution container.

Magnetic bead separating device for nucleic acid extraction the most according to claim 9, it is characterised in that described reaction consumptive material also includes separation sleeve container for hold separation sleeve the most some with described gear shaft.