CN106520547A - Nucleic acid extraction equipment - Google Patents
Nucleic acid extraction equipment Download PDFInfo
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- CN106520547A CN106520547A CN201710026422.XA CN201710026422A CN106520547A CN 106520547 A CN106520547 A CN 106520547A CN 201710026422 A CN201710026422 A CN 201710026422A CN 106520547 A CN106520547 A CN 106520547A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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Abstract
The invention relates to nucleic acid extraction equipment which is used for extracting nucleic acid by the aid of a reaction plate with a plurality of reaction holes and comprises a heating and vibrating device, a temperature control reagent device, a uniform magnetic bead mixing device and a magnetic bead transferring device. The reaction plate can be mounted, heated and vibrated by the heating and vibrating device, so that parts of the reaction holes of the reaction plate can have first preset temperatures, and the other reaction holes of the reaction plate can have second present temperatures; the temperature control reagent device is provided with refrigerating mechanisms and is used for maintaining articles placed on the temperature control reagent device at third preset temperatures; the uniform magnetic bead mixing device is provided with movement mechanisms and is used for uniformly mixing magnetic bead suspension placed on the uniform magnetic bead mixing device; the magnetic bead transferring device is provided with movable magnetic bars, and magnetic beads can be sucked by the magnetic bars to be transferred to preset locations. The nucleic acid extraction equipment has the advantages that manual nucleic acid extraction operation by the aid of magnetic bead processes can be omitted, the nucleic acid extraction equipment is good in stability, and the nucleic acid extraction efficiency can be greatly improved; the nucleic acid extraction equipment is simple in structure and low in cost and has an excellent application prospect.
Description
Technical field
The present invention relates to biotechnological device field, more particularly to a kind of nucleic acid extraction instrument.
Background technology
In recent years, with the high speed development of molecular biology, large quantities of molecular biosciences based on nucleic acid have been emerged
Experiment, such as secondary sequencing, quantitative fluorescent PCR, gene chip, making nucleic acid molecular hybridization etc. are learned, and the integrity of DNA, RNA degrade
Degree, the purity of nucleic acid and concentration etc. all produce more or less impact to molecular biology experiment.In addition include precisely medical treatment
Including, especially emergency treatment application (such as cause of disease quick diagnosis technology) is just being obtained more and more the medical applications based on nucleic acid
Pay attention to and apply, strong influence traditional medical diagnostic method, improve quick diagnosis level.
Method for extracting nucleic acid main at present is paramagnetic particle method, foundation and pellosil centrifugal column identical principle, with nanometer
Technology the surface of superparamagnetic nano particle is improved and surface modification after, be prepared into superparamagnetism silicon oxide nano magnetic
Pearl, the magnetic bead on micro interface can be specifically recognized and efficiently be combined with nucleic acid molecules.Using silicon oxide Nano microsphere
Superparamagnetism, in the presence of guanidine hydrochloride, guanidinium isothiocyanate etc. and externally-applied magnetic field, can be from blood, animal tissue, food, cause of disease
Nucleic acid is isolated in microorganism equal samples.
Paramagnetic particle method nucleic acid extraction can be generally divided into cracking, combination, washing and eluting, however, by manually carrying out
Need to expend long time, affect the efficiency of nucleic acid extraction, and nucleic acid easily degrades, extraction time is long to be affected to be extracted
The quality of nucleic acid.
The content of the invention
Based on this, it is necessary to for it is above-mentioned manually carry out paramagnetic particle method nucleic acid extraction expend the time it is long, nucleic acid extraction effect
A kind of low problem of rate, there is provided simple efficient nucleic acid extraction instrument.
Specific technical scheme is as follows：
A kind of nucleic acid extraction instrument, extracts nucleic acid using the Sptting plate with some reacting holes, and nucleic acid extraction instrument includes：
Heating vibration device, can install and Sptting plate described in heating vibration, make the part reacting hole of the Sptting plate
The first preset temperature is reached, the part reacting hole in other described reacting holes reaches the second preset temperature；
Temperature control reagent device, with refrigeration mechanism, for maintaining the article being positioned on the temperature control reagent device
3rd preset temperature；
Magnetic bead evenly mixing device, with motion, for mixing the suspension containing magnetic beads being positioned on the magnetic bead evenly mixing device；
Magnetic bead transfer device, with moveable magnetic bar magnet, the adsorbable magnetic bead of the bar magnet is with by the magnetic bead
It is transferred to predeterminated position.
When above-mentioned nucleic acid extraction instrument is used, sample and reagent are positioned on temperature control reagent device to maintain low temperature shape
State, suspension containing magnetic beads is positioned on magnetic bead evenly mixing device to keep magnetic bead even suspension, Sptting plate is placed in heating vibration device
On, make partial reaction hole reach the cracking temperature needed for cracking, the part in other reacting holes reaches the eluting needed for eluting
Temperature, the reacting hole of remaining room temperature are then used for washing.First place a sample in the reacting hole for reaching cracking temperature, add cracking
Liquid, with reference to liquid and magnetic bead, lysate, can damage cells structure with reference to liquid, make the abundant separate out of nucleic acid, with the magnetic bead in solution
With reference to the nucleic acid absorption for then being combined by magnetic bead and with magnetic bead by magnetic bead transfer device is transferred in the reacting hole of room temperature and is passed through
Cleaning mixture washing is added to remove residual impurity.Then again magnetic bead and nucleic acid are transferred in the reacting hole for reaching eluting temperature, are led to
Addition nucleic acid of the eluent Dissociative on magnetic bead is crossed, finally magnetic bead is moved away, more than in each step, shaken by heating
Dynamic device dynamic respons plate makes cracking washing eluting fully, and high concentration and highly purified nucleic acid are then stayed in reacting hole.By this
Nucleic acid extraction instrument, eliminating manually carries out the operation of paramagnetic particle method nucleic acid extraction, good stability, and nucleic acid extraction efficiency is significantly
Improve, and the device structure is simple, low cost has a good application prospect.
Wherein in one embodiment, the magnetic bead transfer device includes：
Substrate, the substrate are provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with the magnetosheath of hollow and one end open；
Bar magnet mechanism, the bar magnet mechanism are provided with the bar magnet, and the bar magnet mechanism is installed on the substrate and can be
Under the driving of the substrate actuator, relatively described magnetosheath frame is moved back and forth, to drive the relatively described magnetosheath frame of the bar magnet reciprocal
Move and stretch into or away from the magnetosheath；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are driving the substrate to move
It is dynamic.
Wherein in one embodiment, the substrate is provided with guide rail slide block, and the guide rail slide block includes being fixed on described
The guide rail of substrate and can be connected to be installed on the base with the slide block along the slide block of the slide, the bar magnet mechanism
Plate, the substrate actuator are connected to drive the bar magnet mechanism to move back and forth along the guide rail with the bar magnet mechanism.
Wherein in one embodiment, the bar magnet mechanism includes connecting plate and bar magnet frame, described connecting plate one end and institute
Slide block connection is stated, is connected with the bar magnet frame away from the other end of the slide block, the bar magnet is located at the bar magnet frame.
Wherein in one embodiment, the substrate actuator is provided with substrate drive shaft, and the substrate actuator passes through
The substrate drive shaft is threadedly coupled with the connecting plate, and the substrate actuator can drive the substrate drive shaft to rotate with band
Move the connecting plate to move back and forth along the guide rail.
Wherein in one embodiment, reciprocating liquid relief slide block on the transfer arm, is additionally provided with, the liquid relief is slided
Block is provided with liquid relief part for transfer liquid away from one end of the transfer arm.
Wherein in one embodiment, the heating vibration device includes：
Heating arrangements, can install and heat the Sptting plate, including the first heating component and the second heating component, and described the
One heating component is used to heat the part reacting hole of the Sptting plate and reaches first preset temperature；Second heating
The part reacting hole that component is used to heat in other described reacting holes reaches second preset temperature；
Vibrating mechanism, is connected with the heating arrangements, for driving the heating arrangements to vibrate to drive the Sptting plate
Vibration.
Wherein in one embodiment, the first heating component includes the first support member and the first heating member, described the
Two heating components include that the second support member and the second heating member, first support member and second support member cooperatively form peace
The installation position of the Sptting plate is filled, first heating member is connected to incite somebody to action by first support member with first support member
To the Sptting plate, second heating member is connected to incite somebody to action by second support member with second support member heat transfer
Heat transfer is to the Sptting plate.
Wherein in one embodiment, the first heating component is provided with first heating member communication connection the
One temperature sense part, to detect the temperature of the reacting hole of the first heating component heating, and controls first heating
Part stops or runs；The second heating component is provided with the second temperature induction pieces with second heating member communication connection,
To detect the temperature of the reacting hole of the second heating component heating, and control the second heating member stopping or run.
Wherein in one embodiment, the vibrating mechanism includes drive component and rotary components, the rotary components with
Heating arrangements connection, the drive component be connected with the rotary components with drive the rotary components eccentric rotary so as to
Drive the heating arrangements rotation and vibrate.
Description of the drawings
Fig. 1 is the structural representation of the nucleic acid extraction instrument of an embodiment；
Fig. 2 is the magnetic bead transfer device bar magnet of the nucleic acid extraction instrument shown in Fig. 1 away from structural representation during magnetosheath；
Fig. 3 is the structural representation when magnetic bead transfer device bar magnet of the nucleic acid extraction instrument shown in Fig. 1 stretches into magnetosheath；
Fig. 4 is the structural representation of the heating vibration device of the nucleic acid extraction instrument shown in Fig. 1.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But, the present invention can be realized in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being installed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connection
To another element or may be simultaneously present centering elements.When an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technology used herein and scientific terminology and the technical field for belonging to the present invention
The implication that technical staff is generally understood that is identical.The term for being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that of the invention in limiting.Term as used herein "and/or" includes one or more phases
The arbitrary and all of combination of the Listed Items of pass.
As shown in figure 1, a kind of nucleic acid extraction instrument 600 of a preferred embodiment of the present invention, using with some reacting holes
Sptting plate 200 extract nucleic acid, nucleic acid extraction instrument 600 include heating vibration device 100, magnetic bead transfer device 300, temperature control examination
Agent device 400 and magnetic bead evenly mixing device 500.
Heating vibration device 100 can be installed and heating vibration Sptting plate 200, reach the partial reaction hole of Sptting plate 200
First preset temperature, the partial reaction hole in other reacting holes reach the second preset temperature.Temperature control reagent device 400 has refrigeration
Mechanism, for making the article being positioned on temperature control reagent device 400 maintain the 3rd preset temperature.Magnetic bead evenly mixing device 500 has
There is motion, for mixing the suspension containing magnetic beads being positioned on magnetic bead evenly mixing device.Magnetic bead transfer device 300 has moveable
Magnetic bar magnet 331,331 adsorbable magnetic bead of bar magnet is being transferred to predeterminated position by magnetic bead.
When the nucleic acid extraction instrument 600 of the present embodiment is used, by sample and reagent be positioned on temperature control reagent device 400 with
Low temperature state is maintained, suspension containing magnetic beads are positioned on magnetic bead evenly mixing device 500 to keep magnetic bead even suspension, by Sptting plate 200
It is installed on heating vibration device 100, makes partial reaction hole reach the cracking temperature needed for cracking, in other reacting holes
The eluting temperature needed for eluting point is then reached, the reacting hole of remaining room temperature is then used for washing.First place a sample into and reach cracking temperature
In the reacting hole of degree, add lysate, combine liquid, lysate, structure is can damage cells with reference to liquid, make nucleic acid fully dissociate
Come, the nucleic acid dissociated after adding suspension containing magnetic beads is combined with the magnetic bead in solution, then by magnetic bead transfer device 300 by magnetic bead and
The nucleic acid absorption combined with magnetic bead removes residual impurity by adding cleaning mixture washing in being transferred to the reacting hole of room temperature.Then again
Magnetic bead and nucleic acid are transferred in the reacting hole for reaching eluting temperature, by adding core of the eluent Dissociative on magnetic bead
Magnetic bead is finally moved away by acid, more than in each step, washs cracking by 100 dynamic respons plate 200 of heating vibration device
Fully, high concentration and highly purified nucleic acid are then stayed in reacting hole eluting.By the nucleic acid extraction instrument 600, eliminate artificial
The operation of paramagnetic particle method nucleic acid extraction is carried out manually, good stability, nucleic acid extraction efficiency are greatly improved, and the device structure is simple,
Low cost, has a good application prospect.
Further, as shown in Figures 2 and 3, magnetic bead transfer device 300 includes substrate 310, magnetosheath frame 320, bar magnet mechanism
330 and travel mechanism's (not shown).Substrate 310 is provided with substrate actuator 311, and magnetosheath frame 320 is installed on substrate 310, magnetosheath
Frame 320 is provided with the magnetosheath 321 of hollow and one end open.Bar magnet mechanism 330 is provided with the magnetic bar magnet 331 of tool, bar magnet machine
Structure 330 is installed on substrate 310 and relative magnetosheath frame 320 can move back and forth to drive bar magnet under the driving of substrate actuator 311
331 relative magnetosheath framves 320 are moved back and forth, so that bar magnet 331 is stretched into or away from magnetosheath 321.Travel mechanism has moveable
Transfer arm 341, transfer arm 341 are connected to drive substrate 310 to move with substrate 310.Specifically in the present embodiment, transfer arm 341
Substrate 310 can be driven to move along the axial direction or radial direction of bar magnet 331, it will be understood that move mode not limited to this, can root
Need to be adjusted according to the transfer of magnetic bead.
Thus, in can first placing a sample into the reacting hole of Sptting plate 200, cellularity is destroyed by lysate, with reference to liquid,
The abundant separate out of nucleic acid is made, is combined with the magnetic bead in solution, substrate 310 is driven by the transfer arm 341 of travel mechanism then
Movement makes magnetosheath 321 stretch in reacting hole, then drives 330 motion of bar magnet mechanism to stretch into bar magnet 331 by substrate actuator 311
In magnetosheath 321, magnetic bead and the nucleic acid combined with magnetic bead are then adsorbed on magnetosheath 321.Then moving substrate 310 stretches magnetosheath 321
Enter in the reacting hole for washing, drive 330 motion of bar magnet mechanism bar magnet 331 to be made away from magnetosheath by substrate actuator 311
321, dynamic respons plate 200 makes magnetic bead and nucleic acid depart from magnetosheath 321 to be dispersed in cleaning mixture, and is fully washed by cleaning mixture and remove
Remove residual impurity.Then substrate actuator 311 drives the motion of bar magnet mechanism 330 to make bar magnet 331 stretch into the absorption magnetic of magnetosheath 321 again
Pearl and nucleic acid, then moving substrate 310 magnetosheath 321 is stretched in the reacting hole for eluting, existed by eluent Dissociative
The magnetic bead that eluting is finished is moved away by the nucleic acid on magnetic bead, last moving substrate 310, and high concentration and highly purified nucleic acid are then stayed in
In reacting hole, magnetic bead transfer efficiency is high, and good stability, is remarkably improved nucleic acid extraction efficiency and quality.
Specifically, substrate 310 is provided with guide rail slide block 312, and guide rail slide block 312 includes the guide rail for being fixed on substrate 310
3121 and the slide block 3122 that can slide along guide rail 3121, bar magnet mechanism 330 be connected to be installed on substrate 310, base with slide block 3122
Plate actuator 311 is connected to drive bar magnet mechanism 330 to move back and forth along guide rail 3121 with bar magnet mechanism 330.In the present embodiment,
Substrate actuator 311 is linear stepping motor, is fixed on substrate 310 by substrate actuator bracing frame 3111.
Bar magnet mechanism 330 includes connecting plate 332 and bar magnet frame 333, and 332 one end of connecting plate is connected with slide block 3122, away from
The other end of slide block 3122 is connected with bar magnet frame 333, and bar magnet 331 is located at bar magnet frame 333.
Further, substrate actuator 311 is provided with substrate drive shaft 3112, and substrate actuator 311 is driven by substrate
Axle 3112 is threadedly coupled with connecting plate 332, substrate actuator 311 the substrate drive shaft 311 can be driven to rotate in different directions with
Connecting plate 332 is driven to move back and forth along guide rail 3121.
Preferably, bar magnet frame 333 is provided with magnetic part mounting groove 3331, is provided with magnetic part in magnetic part mounting groove 3331
3332, bar magnet 331 is contacted with magnetic part 3332, is strengthened the magnetic of bar magnet 331 so as to pass through magnetic part 3332, is made magnetic bead energy of adsorption
Power is more preferable.
Preferably, bar magnet mechanism 330 is provided with bar magnet location sensitive plate 334, and substrate 310 is provided with and substrate actuator
The first position induction pieces 313 and second position induction pieces 314 of 311 communication connections, bar magnet mechanism 330 move back and forth predeterminable range
When, bar magnet location sensitive plate 334 is corresponding with first position induction pieces 313 or 314 position of second position induction pieces, so that first
Put induction pieces 313 or 314 control base board actuator 311 of second position induction pieces stops, bar magnet mechanism 330 is reciprocal to transport so as to limit
Dynamic distance, makes bar magnet 331 and the relative distance of magnetosheath 321 control more stable and accurate.In the present embodiment, substrate 310
On be fixed with circuit board 315, first position induction pieces 313 and second position induction pieces 314 are optocoupler, are installed on circuit board 315,
With sensing groove, bar magnet mechanism 330 moves back and forth can be such that bar magnet location sensitive plate 334 stretches in corresponding sensing groove, so that the
One location sensitive part 313 or 314 control base board actuator 311 of second position induction pieces stop, and prevent bar magnet mechanism 330 from continuing original
The motion in direction.
Preferably, 3331 two ends of magnetic part mounting groove are provided with magnetic part fixed plate 3333, magnetic part fixed plate 3333 and magnetic
Property part 3332 abuts magnetic part 3332 is fixed in magnetic part mounting groove 3331, so as to keep stablizing for magnetic part 3332.
Preferably, Fig. 1 is referred to, reciprocating liquid relief slide block 351, liquid relief slide block 351 on transfer arm 341, is additionally provided with
Liquid relief part 352 is provided with for transfer liquid away from one end of transfer arm 341, thus, transfer arm 341 and liquid relief slide block can be passed through
351 movement liquid relief parts 352, sample, reaction reagent and suspension containing magnetic beads are transferred in corresponding reacting hole, further save artificial
Operation, improves extraction efficiency.
In the present embodiment, the quantity of bar magnet 331 is 12, and 12 magnetosheaths 321 are corresponded with bar magnet 331, bar magnet 331
It is arranged in a linear, corresponding with the reacting hole of 96 orifice plates, the quantity of liquid relief part 352 is 6, is arranged in a linear, with temperature control reagent
Reagent on device 400 is corresponding with sample position.It is appreciated that the quantity and arrangement mode of bar magnet 331 and liquid relief part 352 can be with
Adjust as needed, to adapt to different Sptting plates 200 and demand.
Further, a diameter of 2mm-10mm of magnetosheath 321, the sample volume for extracting are minimum up to 20uL, energy
Sample and cost are saved enough.
Specifically, as shown in figure 4, heating vibration device 100 includes heating arrangements 110 and vibrating mechanism 120.Heating arrangements
110 can install and reacting by heating plate 200, and heating arrangements 110 include the first heating component 111 and the second heating component 112, and first
Heating component 111 is used for the partial reaction hole of reacting by heating plate 200 and reaches the first preset temperature, and the second heating component 112 is used for
Partial reaction hole in other reacting holes of reacting by heating plate 200 reaches the second preset temperature.Vibrating mechanism 120 and heating arrangements
110 connections, for driving heating arrangements 110 to vibrate to drive Sptting plate 200 to vibrate.
Thus, only Sptting plate 200 need to be placed on heating arrangements 110, the first heating component 111 is by Sptting plate 200
The heating of partial reaction hole reaches the cracking temperature needed for cracking, and the second heating component 112 is then by the part in other reacting holes
Heating reaches the eluting temperature needed for eluting, and the reacting hole of remaining room temperature is then used for washing.Sample is first inserted and reaches cracking temperature
Reacting hole in, by lysate, with reference to liquid destroy cellularity, make the abundant separate out of nucleic acid, so as to be combined with magnetic bead, so
Residual impurity is removed by cleaning mixture washing during magnetic bead to be transferred to the reacting hole of room temperature afterwards, then is transferred to reach by magnetic bead and is washed
In the reacting hole of de- temperature, by nucleic acid of the eluent Dissociative on magnetic bead, then magnetic bead is moved away, each step more than
In, vibrating mechanism 120 drives heating arrangements 110 to vibrate so as to crack washing eluting fully, high concentration and highly purified nucleic acid are then
Stay in reacting hole, eliminate, be then cooled to room temperature, be again heated to eluting temperature it is very long when
Between, magnetic bead transfer distance is short, without the need for manual dynamic respons plate 200, substantially increases nucleic acid extraction efficiency and quality.
Specifically, the first heating component 111 includes the first support member 1111 and the first heating member 1112, the second heating component
112 include that the second support member 1121 and the second heating member 1122, the first support member 1111 and the second support member 1121 are cooperatively formed
The installation position of Sptting plate 200 is installed, the first heating member 1112 is connected with the first support member 1111 with by the first support member 1111
Sptting plate 200 is transferred heat to, the second heating member 1122 is connected with the second support member 1121 with by the second support member 1121
Transfer heat to Sptting plate 200.
In the present embodiment, Sptting plate 200 is 96 square orifice plates, and the first support member 1111 and the second support member 1121 are equal
It is the bracing frame with square mounting groove, it is common to coordinate to form the installation position for installing Sptting plate 200,1111 He of the first support member
Second support member 1121 is contacted with the opposite end of Sptting plate 200 respectively, relative so as to heat to be transferred to Sptting plate 200 respectively
Four row's reacting holes at two ends, middle four rows reacting hole are then room temperature.It is appreciated that the type not limited to this of Sptting plate 200, such as
Various different materials and the structures such as 384 orifice plates, 108 orifice plates, Tissue Culture Plate, PCR plate, the first support member 1111 and second
The shape of support member 1121 can be according to the Adjusting Shape of Sptting plate 200.Preferably, the first heating member 1112 and the second heating member
1122 surfaces scribble thermally conductive grease, improve heat transfer efficiency.
Further, the first heating component 111 is provided with the first temperature sense with the communication connection of the first heating member 1112
Part 1113, to detect the temperature of the reacting hole of the heating of the first heating component 111, and controls the first heating member 1112 and stops or transport
OK.Second heating component 112 is provided with the second temperature induction pieces 1123 with the communication connection of the second heating member 1122, to detect the
The temperature of the reacting holes of the two heating heating of components 112, and control the second heating member 1122 and stop or run.Thus, work as first adding
The reacting hole temperature of the heating of hot component 111 reaches the first preset temperature such as cracking temperature, and the first temperature sense part 1113 can send
Signal control the first heating member 1112 stops heating, otherwise continues heating；When the reacting hole temperature of the second heating heating of component 112
Reach the second preset temperature such as eluting temperature, second temperature induction pieces 1123 can sending signal control the second heating member 1122 and stop
Heating, otherwise continues heating, so that corresponding reacting hole maintains corresponding temperature.
Specifically, vibrating mechanism 120 includes drive component 121 and rotary components 122, rotary components 122 and heating arrangements
110 connections, drive component 121 are connected to drive 122 eccentric rotary of rotary components so as to drive heating arrangements with rotary components 122
110 rotations and vibration.
Further, drive component 121 includes rotation driving axle 1211 and drives the rotation of the rotation of rotation driving axle 1211
Turn actuator 1212, rotary components 122 include gripper shoe 1221 and coupling spindle 1222, and heating arrangements 110 are installed on gripper shoe
1221,1222 one end of coupling spindle is connected with gripper shoe 1221, and the other end is connected with rotation driving axle 1211, coupling spindle 1222 turn
The disorder of internal organs heart deviates from the center of rotation of rotation driving axle 1211.More specifically, coupling spindle 1222 pass through set screw and D type holes with
Rotation driving axle 1211 connects, on coupling spindle 1222 near 1212 one end of rotary drive be arranged with successively lower bearing 1223,
Sleeve 1224 and upper bearing (metal) 1225, are arranged with rotary shaft 1226, rotary shaft on lower bearing 1223, sleeve 1224 and upper bearing (metal) 1225
Swivel mount 1227 is arranged with 1226, swivel mount 1227 is rotated together with rotary shaft 1226 by D type holes, swivel mount 1227 away from
Side of the one end of rotary shaft 1226 with gripper shoe 1221 away from heating arrangements 110 is connected, thus, by rotation driving axle 1211
Rotary drive is to gripper shoe 1221, and then drives heating arrangements 110 to rotate.
Preferably, heat insulating mattress 1228 is provided between heating arrangements 110 and gripper shoe 1221, so as to heating arrangements can be prevented
110 heat transfer is to gripper shoe 1221, and improves the efficiency of heating surface.
Further, fixed plate 1213 is connected with rotary drive 1212, fixed plate 1213 is provided with is driven with rotation
The coupling spindle location sensitive part 1214 of the communication connection of moving part 1212, coupling spindle location sensitive part 1214 can send induced signal to control
Rotary drive processed 1212 stops, and coupling spindle 1222 is provided with and 1214 corresponding coupling spindle tablet of coupling spindle location sensitive part
1215 so that coupling spindle location sensitive part 1214 produces induced signal.In the present embodiment, coupling spindle location sensitive part 1214 has
There is sensing groove, coupling spindle tablet 1215 is included to the extension 12151 and and extension extended away from 1222 direction of coupling spindle
12151 occlusion parts 12152 for forming an angle, coupling spindle 1222 are rotarily driven after coupling spindle tablet 1215 rotates to an angle
Occlusion part 12152 can be made to stretch into sensing groove, so that coupling spindle location sensitive part 1214 produces actuated signal control rotation driving
Part 1212 stops.Thus, when needing to stop vibrating mechanism 120, only need to start coupling spindle location sensitive part 1214, treat occlusion part
12152 stretch into sensing groove, you can stop the rotation actuator 1212.
Preferably, fixed plate 1213 is provided with damping shock absorption part (not shown), to prevent the gripper shoe in running
The 1221 inclined angles of vibration are excessive to cause the liquid in Sptting plate 200 to spill.Specifically, damping shock absorption part is some to support
Flexible column such as rubber column, plastic column etc. that plate 1221 extends, or the damping washer such as rubber washer around coupling spindle 1222 etc.,
Structure not limited to this.
Preferably, rotary drive 1212 is provided with damping and silencing pad 1216, fixed plate 1213 and damping and silencing pad 1216
Connect to be connected with rotary drive 1212 by damping and silencing pad 1216, so as to weakening the vibration of fixed plate 1213 and sending
Sound.
Preferably, coupling spindle 1222 is eccentric shaft, therefore partial center rotation can occur when rotating, and can not only transmit rotation,
Revolution can also be transmitted simultaneously, so that 110 whirling vibration of heating arrangements, improves Blending Efficiency of Blending.
The nucleic acid extraction instrument 600 of the present embodiment passes through heating vibration device 100, magnetic bead transfer device 300, temperature control reagent
The cooperation of device 400 and magnetic bead evenly mixing device 500 eliminates the operation for manually carrying out paramagnetic particle method nucleic acid extraction, stability
Good, nucleic acid extraction efficiency is greatly improved, and the device structure is simple, and low cost has a good application prospect.
Each technical characteristic of embodiment described above arbitrarily can be combined, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more concrete and detailed, but and
Therefore can not be construed as limiting the scope of the patent.It should be pointed out that for one of ordinary skill in the art comes
Say, without departing from the inventive concept of the premise, some deformations and improvement can also be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (10)
1. a kind of nucleic acid extraction instrument, extracts nucleic acid using the Sptting plate with some reacting holes, it is characterised in that the nucleic acid
Extraction equipment includes：
Heating vibration device, can install and Sptting plate described in heating vibration, reach the part reacting hole of the Sptting plate
First preset temperature, the part reacting hole in other described reacting holes reach the second preset temperature；
Temperature control reagent device, with refrigeration mechanism, for making the article being positioned on the temperature control reagent device maintain the 3rd
Preset temperature；
Magnetic bead evenly mixing device, with motion, for mixing the suspension containing magnetic beads being positioned on the magnetic bead evenly mixing device；
Magnetic bead transfer device, with moveable magnetic bar magnet, the adsorbable magnetic bead of the bar magnet is so that the magnetic bead to be shifted
To predeterminated position.
2. nucleic acid extraction instrument according to claim 1, it is characterised in that the magnetic bead transfer device includes：
Substrate, the substrate are provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with the magnetosheath of hollow and one end open；
Bar magnet mechanism, the bar magnet mechanism are provided with the bar magnet, and the bar magnet mechanism is installed on the substrate and can be described
Under the driving of substrate actuator, relatively described magnetosheath frame is moved back and forth, to drive the relatively described magnetosheath frame of the bar magnet to move back and forth
And stretch into or away from the magnetosheath；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are driving the substrate movement.
3. nucleic acid extraction instrument according to claim 2, it is characterised in that the substrate is provided with guide rail slide block, described
Guide rail slide block includes the guide rail for being fixed on the substrate and can be along the slide block of the slide, the bar magnet mechanism and the cunning
Block connects to be installed on the substrate, and the substrate actuator is connected to drive the bar magnet mechanism along institute with the bar magnet mechanism
State guide rail reciprocating motion.
4. nucleic acid extraction instrument according to claim 3, it is characterised in that the bar magnet mechanism includes connecting plate and bar magnet
Frame, described connecting plate one end are connected with the slide block, are connected with the bar magnet frame away from the other end of the slide block, the bar magnet
Located at the bar magnet frame.
5. nucleic acid extraction instrument according to claim 4, it is characterised in that the substrate actuator is provided with substrate driving
Axle, the substrate actuator are threadedly coupled with the connecting plate by the substrate drive shaft, and the substrate actuator can drive
The substrate drive shaft rotates to drive the connecting plate to move back and forth along the guide rail.
6. nucleic acid extraction instrument according to claim 2, it is characterised in that be additionally provided with reciprocable on the transfer arm
Liquid relief slide block, the liquid relief slide block is provided with liquid relief part for transfer liquid away from one end of the transfer arm.
7. nucleic acid extraction instrument according to claim 1, it is characterised in that the heating vibration device includes：
Heating arrangements, can install and heat the Sptting plate, including the first heating component and the second heating component, and described first adds
Hot component is used to heat the part reacting hole of the Sptting plate and reaches first preset temperature；The second heating component
The part reacting hole for heating in other described reacting holes reaches second preset temperature；
Vibrating mechanism, is connected with the heating arrangements, for driving the heating arrangements to vibrate to drive the reaction panel vibration.
8. nucleic acid extraction instrument according to claim 7, it is characterised in that the first heating component includes the first support
Part and the first heating member, the second heating component include the second support member and the second heating member, first support member and institute
State the second support member and cooperatively form the installation position for installing the Sptting plate, first heating member is connected with first support member
To transfer heat to the Sptting plate by first support member, second heating member is connected with second support member
To transfer heat to the Sptting plate by second support member.
9. nucleic acid extraction instrument according to claim 8, it is characterised in that the first heating component be provided with it is described
First temperature sense part of the first heating member communication connection, to detect the temperature of the reacting hole of the first heating component heating
Degree, and control the first heating member stopping or run；The second heating component is provided with and is communicated with second heating member
The second temperature induction pieces of connection, to detect the temperature of the reacting hole of the second heating component heating, and control described
Second heating member stops or runs.
10. nucleic acid extraction instrument according to claim 7, it is characterised in that the vibrating mechanism include drive component and
Rotary components, the rotary components are connected with the heating arrangements, and the drive component is connected to drive with the rotary components
The rotary components eccentric rotary is rotated and vibration so as to drive the heating arrangements.
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| CN201710026422.XA CN106520547B (en) | 2017-01-13 | 2017-01-13 | Nucleic acid extraction instrument |
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| CN201710026422.XA CN106520547B (en) | 2017-01-13 | 2017-01-13 | Nucleic acid extraction instrument |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107988045A (en) * | 2018-01-08 | 2018-05-04 | 深圳市朗司医疗科技有限公司 | A kind of full-automatic nucleic acid purification system based on paramagnetic particle method |
| CN108642045A (en) * | 2018-05-08 | 2018-10-12 | 绍兴迅敏康生物科技有限公司 | A kind of bar magnet set |
| CN109266547A (en) * | 2018-11-27 | 2019-01-25 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
| CN110945363A (en) * | 2017-07-21 | 2020-03-31 | Seegene株式会社 | Module for transferring magnetic beads, automated system comprising the module, and method for extracting nucleic acid using the module |
| CN111218398A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1649931A1 (en) * | 2004-10-01 | 2006-04-26 | Hitachi High-Technologies Corporation | Chemical analyzing apparatus for biological substances |
| CN101748175A (en) * | 2008-12-05 | 2010-06-23 | 天津生物芯片技术有限责任公司 | Method for simply extracting bacteria DNA from food samples |
| CN103725603A (en) * | 2007-07-13 | 2014-04-16 | 汉迪实验室公司 | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN104271765A (en) * | 2012-02-13 | 2015-01-07 | 纽莫德克斯莫勒库拉尔公司 | System and method for processing and detecting nucleic acids |
| CN104450498A (en) * | 2014-12-16 | 2015-03-25 | 嘉兴凯实生物科技有限公司 | Nucleic acid extractor |
| CN104479993A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Nucleic acid extraction device for nucleic acid extractor |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN206408241U (en) * | 2017-01-13 | 2017-08-15 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Nucleic acid extraction instrument |
-
2017
- 2017-01-13 CN CN201710026422.XA patent/CN106520547B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1649931A1 (en) * | 2004-10-01 | 2006-04-26 | Hitachi High-Technologies Corporation | Chemical analyzing apparatus for biological substances |
| CN103725603A (en) * | 2007-07-13 | 2014-04-16 | 汉迪实验室公司 | Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| CN101748175A (en) * | 2008-12-05 | 2010-06-23 | 天津生物芯片技术有限责任公司 | Method for simply extracting bacteria DNA from food samples |
| CN104271765A (en) * | 2012-02-13 | 2015-01-07 | 纽莫德克斯莫勒库拉尔公司 | System and method for processing and detecting nucleic acids |
| CN103756871A (en) * | 2013-12-30 | 2014-04-30 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN103897987A (en) * | 2014-02-18 | 2014-07-02 | 中国农业大学 | Automatic extraction device and method of nucleic acid based on nano magnetic beads |
| CN103789198A (en) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | Full automatic instrument for extracting nucleic acids |
| CN104450498A (en) * | 2014-12-16 | 2015-03-25 | 嘉兴凯实生物科技有限公司 | Nucleic acid extractor |
| CN104479993A (en) * | 2014-12-16 | 2015-04-01 | 嘉兴凯实生物科技有限公司 | Nucleic acid extraction device for nucleic acid extractor |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN206408241U (en) * | 2017-01-13 | 2017-08-15 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Nucleic acid extraction instrument |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110945363A (en) * | 2017-07-21 | 2020-03-31 | Seegene株式会社 | Module for transferring magnetic beads, automated system comprising the module, and method for extracting nucleic acid using the module |
| US11674971B2 (en) | 2017-07-21 | 2023-06-13 | Seegene, Inc. | Modules for transferring magnetic beads, automated system comprising the same and method for nucleic acid extraction using the same |
| CN110945363B (en) * | 2017-07-21 | 2024-04-16 | Seegene株式会社 | A module for transferring magnetic beads, an automated system including the module, and a method of extracting nucleic acid using the module |
| CN107988045A (en) * | 2018-01-08 | 2018-05-04 | 深圳市朗司医疗科技有限公司 | A kind of full-automatic nucleic acid purification system based on paramagnetic particle method |
| CN107988045B (en) * | 2018-01-08 | 2024-01-19 | 深圳市朗司医疗科技有限公司 | Full-automatic nucleic acid purification system based on magnetic bead method |
| CN108642045A (en) * | 2018-05-08 | 2018-10-12 | 绍兴迅敏康生物科技有限公司 | A kind of bar magnet set |
| CN109266547A (en) * | 2018-11-27 | 2019-01-25 | 嘉兴领宇科技有限公司 | Nucleic acid-extracting apparatus |
| CN111218398A (en) * | 2020-03-25 | 2020-06-02 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
| CN111218398B (en) * | 2020-03-25 | 2024-02-13 | 广州高盛生物科技股份有限公司 | Magnetic bead extraction module and extraction method |
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