CN101205246B - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
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- CN101205246B CN101205246B CN2006101654427A CN200610165442A CN101205246B CN 101205246 B CN101205246 B CN 101205246B CN 2006101654427 A CN2006101654427 A CN 2006101654427A CN 200610165442 A CN200610165442 A CN 200610165442A CN 101205246 B CN101205246 B CN 101205246B
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- tube stand
- nucleic acid
- sleeve
- tight plate
- bearing
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Abstract
The invention relates to a nucleic acid extracting device, which is characterized in that the nucleic acid extracting device comprises an oscillator component, a sample holder component, a control system and a frame; the oscillator component comprises an electric motor, an eccentric sleeve connected on the output shaft of the electric motor, a bearing connected on the eccentric shaft of the eccentric sleeve and a bearing sleeve connected on the external ring of the bearing; a threaded sleeve integrated with the bearing sleeve is arranged at the top of the bearing sleeve and a rotation stopping sleeve is arranged on the retaining plate of the electric motor; a plurality of extension springs are connected between the rotation stopping sleeve and the bearing sleeve; the sample holder component comprises a test tube stand, a plurality of sample tubes inserted and arranged on the test tube stand and a scotch yoke arranged at the top of the sample tube; the top of the scotch yoke is connected with a locking cover and the top of the locking cover is connected with a locking handle; the bottom screw rod of the locking handle passes through the locking cover, the scotch yoke and the test tube stand so as to be fixed in the threaded sleeve of the oscillator component and the scotch yoke is elastically connected with the test tube stand. The invention can be used for extracting the nucleic acids of the microorganicsms such as the bacteria (G+ bacteria, G-bacteria, mycobacterium), fungi (yeast, white candida), viruses, etc., including the extraction of the RNA.
Description
Technical field
The present invention relates to a kind of nucleic acid-extracting apparatus, particularly about a kind of nucleic acid-extracting apparatus of realizing sample dissociation by the mode of physics concussion in conjunction with certain reagent.
Background technology
Life science is an experimental science, and a large amount of Bioexperiment and clinical diagnosis be unable to do without specimen preparation, nucleic acid be extracted in extensive application in the specimen preparation.Along with development of biology, the sample size that clinical diagnosis at present and Bioexperiment are handled is often very big.Traditional nucleic acids for preparation mode is that testing sample is added in the reagent that contains with chemical reagent or lyase, reaches the preparation of sample of nucleic acid by chemical reaction.This method has harmful toxic matter, and specimen preparation is consuming time big, and efficient is low, and the sample of processing is few, and many shortcomings such as crossed contamination are arranged, and is difficult to satisfy high speed, large batch of nucleic acid extraction requirement gradually.The sample size of existing nucleic acid extraction instrument single treatment is less, and the system architecture complexity costs an arm and a leg, and extracts length consuming time, usually needs troublesome operation steps such as absorption, washing, wash-out.
Summary of the invention
At the problems referred to above, the purpose of this invention is to provide a kind of many samples and can do oscillating operation simultaneously, and the time spent is few, efficient height, the nucleic acid-extracting apparatus of high conformity.
For achieving the above object, the present invention takes following technical scheme: a kind of nucleic acid-extracting apparatus, it is characterized in that: it comprises oscillator assembly, the specimen holder assembly that connects described oscillator assembly, the Controlling System of described oscillator assembly, and the support that described oscillator assembly and Controlling System are installed; Described oscillator assembly comprises motor, be connected the eccentric bushing on the described motor output shaft, be connected the bearing on the excentric shaft of described eccentric bushing, and the bearing housing that is connected described bearing outer ring, described bearing housing top have one with the thread bush of its one, one spline cover is set on the retaining plate of described motor, is connected with some extension springs between described spline cover and the bearing housing; Described specimen holder assembly comprises test-tube stand, be plugged in the some sample hoses on the described test-tube stand, be arranged on a pressing tight plate at described sample hose top, the top of described pressing tight plate connects a tightening cover, described tightening cover top connects a set lever, the bottom screw rod of described set lever passes described tightening cover, pressing tight plate, test-tube stand, is fixed in the thread bush of described oscillator assembly, and elasticity is connected between described pressing tight plate and the described test-tube stand.
On the eccentric bushing in the described oscillator assembly, the axis of the eccentric orfice that is connected with described motor output shaft and the excentric shaft that is wholely set all is positioned at a side of described eccentric bushing medullary ray, and a distance at interval between the two.
Described pressing tight plate is connected with elasticity between the described test-tube stand, comprise that two symmetric ends fixedly connected and that protrude out described pressing tight plate bottom have the joint pin of flange, correspondence is arranged on the arcuate socket of two convergents on the described test-tube stand, be set in spring and spring adjustment sheet on the described set lever screw rod, described two joint pins pass the stub end of described two arcuate sockets, after described set lever rotates, be positioned at the pin head end of described two arcuate sockets.
The present invention is owing to take above technical scheme, and it has the following advantages: 1, because the present invention can load the dozens of sample hose simultaneously, solved the problem of time-consuming, the inefficiency of traditional method for extracting nucleic acid.2, use a collection of sample size of apparatus of the present invention many, each sample hose shock range is identical, has realized the homogeneous preparation of multiple sample, and has reduced the crossed contamination in the specimen preparation process.3, the present invention is highly sensitive, extracts bacterial nucleic acid, gets 1ul (microlitre) and makes PCR (polymerase chain reaction, Polymerase Chain Reaction)), susceptibility is 10
3Individual bacterium/100 μ l.4, versatility of the present invention is good, and different biology, different strains, different detection gene, different sample (liquid culture or solid culture), different experiments batch operation step are identical, the experimental repeatability height, and extraction efficiency is all identical between pipe.5, the present invention extracts the good stability of nucleic acid, and after 40 days and 60 days, its detection sensitivity does not have noticeable change to the DNA of extraction in 4 ℃ of preservations.6, the present invention is simple in structure, and is easy to operate, and security is good, need not enzyme and harmful chemical reagent.The present invention can be used for extracting bacterium class (G+ bacterium, G-bacterium, mycobacterium) fungi (yeast, Candida albicans), and microbial nucleic acids such as virus comprise the extraction of RNA.
Description of drawings
Fig. 1 is a structural representation of the present invention
Fig. 2 is a vibrator assembly schematic perspective view of the present invention
Fig. 3 is a vibrator assembly connection diagram of the present invention
Fig. 4 is a specimen holder assembly schematic perspective view of the present invention
Fig. 5 is that sample hose of the present invention is plugged in the schematic perspective view on the test-tube stand
Fig. 6 is a tightening cover schematic perspective view of the present invention
Fig. 7 is a pressing tight plate schematic perspective view of the present invention
Fig. 8 is a set lever schematic perspective view of the present invention
Fig. 9 is set lever of the present invention, tightening cover and pressing tight plate connection diagram
Figure 10 is vibrator assembly of the present invention and specimen holder assembly connection diagram
Figure 11 is controller of the present invention and speed regulator schematic perspective view
Embodiment
The present invention will be described in detail below in conjunction with drawings and Examples.
As shown in Figure 1, the present invention includes oscillator assembly 10, specimen holder assembly 20, Controlling System 50 is installed the support 60 of oscillator assembly 10 and Controlling System 50.
As Fig. 2, shown in Figure 3, oscillator assembly 10 of the present invention comprises motor 11, eccentric bushing 12, bearing 13, bearing housing 14, spline cover 15 and extension spring 16.Has an eccentric orfice that is connected with motor 11 on the eccentric bushing 12, the top of eccentric bushing 12 has an excentric shaft 17 of one, excentric shaft 17 and eccentric orfice are all near a side of eccentric bushing 12 medullary rays, and keep an eccentric distance between the two, when eccentric bushing 12 was done eccentric rotary under the drive of motor 11, the other end of eccentric bushing 12 medullary rays can play the effect of counterweight like this.The inner ring of excentric shaft 17 fixedly connected bearings 13, the fixedly connected bearing housing 14 in the outer ring of bearing 13, the top of bearing housing 14 be provided with one with the thread bush 18 of its one, thread bush 18 has internal thread, is used to connect specimen holder assembly 20.Spline cover 15 is connected on the motor fixing plate 19, the respectively fixedly connected spline cover 15 of one end of four extension springs 16, the other end of four extension springs 16 is the edge on fixedly connected bearing housing 14 tops respectively, thereby make bearing housing 14 be subjected to the drawing force of a flexibility, making it do one " O " type track along motor output shaft with excentric shaft 17 rotates, and self does not rotate around excentric shaft 17, promptly only does revolution, not rotation.
As Fig. 4, shown in Figure 5, specimen holder assembly 20 of the present invention comprises test-tube stand 21, sample hose 22, tightening cover 23, pressing tight plate 24 and set lever 25.Be inserted with some sample hoses 22 on the test-tube stand 21, test-tube stand 21 middle parts are provided with a centre hole 26 and two arcuate sockets 27.
As Fig. 6, shown in Figure 7, tightening cover 23 centers are provided with centre hole 28, are provided with two ring through hole 29 around the centre hole 28, comprise symmetrically arranged two stepped holes 30 outside in the ring through hole 29.Corresponding with tightening cover 23, pressing tight plate 24 is provided with centre hole 31 and through hole 32,33 and screw 34.
As Fig. 8, Fig. 9, shown in Figure 10, has one section screw rod 35 on the extension bar of set lever 25 bottoms, screw rod 35 is passed the centre hole 28,31 of tightening cover 23 and fixed tight plate 24, and the through hole 29,33 that passes inner ring with screw is fixed on pressing tight plate 23 and tightening cover 24 in the screw 36 on the set lever 25.In a pair of stepped hole 30 on tightening cover 23, connect two with two screws 37 and pass the joint pin 39 that through hole 32 on the pressing tight plate 24 and bottom have flange 38, pass through hole 29 on the tightening cover 23 with screw 40 again and be fixed in the screw 34 on the pressing tight plate 24; A sheathed spring 41 on the screw rod 35 of set lever 25, and the spring adjustment sheet 42 that is threaded, fix a nut 43 then, again screw rod 35 is passed the centre hole 26 of specimen holder 21, with the flange 38 on two joint pins 39 of pressing tight plate 26 bottoms, insert the stub end of two arcuate sockets 27 of test-tube stand 21, rotational lock handle 25, just the microcephaly that joint pin 39 can be rotated to arcuate socket 27 holds the location, specimen holder 21 and pressing tight plate 26 elasticity are linked together, regulating spring adjustment sheet 42 makes specimen holder 21 and pressing tight plate 24 hold out against by spring 41 elasticity, so that the random axial float of sample hose 22; Screw rod 35 ends with set lever 25 are screwed in the thread bush 18 at oscillator assembly 10 tops at last, and oscillator assembly 10 and specimen holder assembly 20 are fixed together.Above-mentioned specimen holder 21 is connected also with pressing tight plate 26 elasticity can take alternate manner.
As shown in Figure 1, the motor 11 of oscillator assembly 10 of the present invention is bolted to connection in a housing 44, and housing 44 is bolted to connection again on base 60.Base 60 has certain weight, and integral body is not shifted during in order to the running of assurance apparatus of the present invention, and operation stability also can be fixed on base 60 on the ground.
As shown in figure 11, the control in the Controlling System 50 of the present invention comprises a housing 51, is arranged on an intravital timer 52 of shell and a speed regulator 53.Housing 51 is fixed on the base 60, and timer 52 can be the electronic timer or the mechanical timer of control such as micro-chip.Speed regulator 53 also is arranged in the housing 51, the rotating speed of may command motor 11, thereby the adjustment of realization vibration intensity of the present invention.The pilot circuit of timer 52, speed regulator 52 and motor 11 is a prior art all, does not repeat them here.
When the present invention is turned round, the output shaft of motor 11 drives eccentric bushing 12 and rotates, excentric shaft 17 on the eccentric bushing 12 drives bearing housing 14 and the sample assembly 20 on it is done the rotation of one " O " type track around the output shaft of motor 11, sample hose 22 obtains vibration when rotating with specimen holder assembly 20, nucleic acid is extracted by quick cracking.After operation was finished, rotation lock handle 25 just can make two joint pins 39 turn to the stub end of arcuate socket 27, open pressing tight plate 24, take out the sample hose 22 that cracking are finished from specimen holder 21, change new sample hose 22 again after, cover pressing tight plate 24, carry out the operation of next cycle.
Claims (3)
1. nucleic acid-extracting apparatus, it is characterized in that: it comprises oscillator assembly, connects the specimen holder assembly of described oscillator assembly, the Controlling System of described oscillator assembly, and the support that described oscillator assembly and Controlling System are installed;
Described oscillator assembly comprises motor, be connected the eccentric bushing on the described motor output shaft, be connected the bearing on the excentric shaft of described eccentric bushing, and the bearing housing that is connected described bearing outer ring, described bearing housing top have one with the thread bush of its one, one spline cover is set on the retaining plate of described motor, is connected with some extension springs between described spline cover and the bearing housing;
Described specimen holder assembly comprises test-tube stand, be plugged in the some sample hoses on the described test-tube stand, be arranged on a pressing tight plate at described sample hose top, the top of described pressing tight plate connects a tightening cover, described tightening cover top connects a set lever, the bottom screw rod of described set lever passes described tightening cover, pressing tight plate, test-tube stand, is fixed in the thread bush of described oscillator assembly, and spring is connected between described pressing tight plate and the described test-tube stand.
2. a kind of nucleic acid-extracting apparatus as claimed in claim 1, it is characterized in that: on the eccentric bushing in the described oscillator assembly, the axis of the eccentric orfice that is connected with described motor output shaft and the excentric shaft that is wholely set all is positioned at a side of described eccentric bushing medullary ray, and a distance at interval between the two.
3. a kind of nucleic acid-extracting apparatus as claimed in claim 1 or 2, it is characterized in that: described pressing tight plate is connected with spring between the described test-tube stand, comprise that two symmetric ends fixedly connected and that protrude out described pressing tight plate bottom have the joint pin of flange, correspondence is arranged on the arcuate socket of two convergents on the described test-tube stand, be set in spring and spring adjustment sheet on the described set lever screw rod, described two joint pins pass the stub end of described two arcuate sockets, after described set lever rotates, be positioned at the pin head end of described two arcuate sockets.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101654427A CN101205246B (en) | 2006-12-20 | 2006-12-20 | Nucleic acid extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101654427A CN101205246B (en) | 2006-12-20 | 2006-12-20 | Nucleic acid extraction device |
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| Publication Number | Publication Date |
|---|---|
| CN101205246A CN101205246A (en) | 2008-06-25 |
| CN101205246B true CN101205246B (en) | 2011-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101654427A Active CN101205246B (en) | 2006-12-20 | 2006-12-20 | Nucleic acid extraction device |
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| CN (1) | CN101205246B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847573A (en) * | 2012-08-29 | 2013-01-02 | 杭州安杰思医学科技有限公司 | Constant-temperature metal bath oscillation mechanism |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232476A (en) * | 2014-09-17 | 2014-12-24 | 深圳市宝安区沙井人民医院 | High-channel nucleic acid extraction and purification device |
| CN105543089A (en) | 2016-03-08 | 2016-05-04 | 湖南圣湘生物科技有限公司 | Magnetic bead based nucleic acid extraction device and method |
| CN106318847A (en) * | 2016-08-22 | 2017-01-11 | 杭州奥盛仪器有限公司 | Nucleic acid extraction equipment capable of eccentric oscillation |
| CN107560924A (en) * | 2017-10-13 | 2018-01-09 | 孔治 | A kind of suspended matter mechanical mixing apparatus and the mixer using the device |
| CN113738814B (en) * | 2021-08-02 | 2022-12-02 | 扬州大学 | Multidirectional damping and buffering device for extracting centrifuge tube in centrifuge and using method of multidirectional damping and buffering device |
| CN114192030B (en) * | 2022-02-18 | 2022-05-03 | 中国人民解放军总医院第八医学中心 | Blood sampling of hematology branch of academic or vocational study shakes even device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1558951A (en) * | 2001-09-28 | 2004-12-29 | 株式会社益力多本社 | Nucleic acid extraction device |
| CN200992526Y (en) * | 2006-12-20 | 2007-12-19 | 博奥生物有限公司 | Nucleic acid extracting device |
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2006
- 2006-12-20 CN CN2006101654427A patent/CN101205246B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1558951A (en) * | 2001-09-28 | 2004-12-29 | 株式会社益力多本社 | Nucleic acid extraction device |
| CN200992526Y (en) * | 2006-12-20 | 2007-12-19 | 博奥生物有限公司 | Nucleic acid extracting device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102847573A (en) * | 2012-08-29 | 2013-01-02 | 杭州安杰思医学科技有限公司 | Constant-temperature metal bath oscillation mechanism |
| CN102847573B (en) * | 2012-08-29 | 2014-10-29 | 杭州安杰思医学科技有限公司 | constant-temperature metal bath oscillation mechanism |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101205246A (en) | 2008-06-25 |
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Owner name: CAPITALBIO CORPORATION CO., LTD. Free format text: FORMER NAME: CAPITALBIO CORPORATION |
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Address after: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee after: CAPITALBIO CORPORATION Patentee after: Tsinghua University Address before: 102206 Beijing City, Changping District Life Science Park Road No. 18 Patentee before: Capitalbio Corporation Patentee before: Tsinghua University |