CN106675991A - Magnetic bead transfer device and nucleic acid extraction equipment comprising same - Google Patents
Magnetic bead transfer device and nucleic acid extraction equipment comprising same Download PDFInfo
- Publication number
- CN106675991A CN106675991A CN201710026417.9A CN201710026417A CN106675991A CN 106675991 A CN106675991 A CN 106675991A CN 201710026417 A CN201710026417 A CN 201710026417A CN 106675991 A CN106675991 A CN 106675991A
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- Prior art keywords
- substrate
- bar magnet
- magnetic
- magnetic bead
- transfer device
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- 230000005291 magnetic effect Effects 0.000 title claims abstract description 110
- 239000011324 bead Substances 0.000 title claims abstract description 59
- 238000012546 transfer Methods 0.000 title claims abstract description 47
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 37
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 37
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 37
- 238000000605 extraction Methods 0.000 title claims abstract description 17
- 239000000758 substrate Substances 0.000 claims abstract description 87
- 230000006698 induction Effects 0.000 claims description 20
- 238000004891 communication Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims 1
- 230000005389 magnetism Effects 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000013508 migration Methods 0.000 description 3
- 230000005012 migration Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229910052814 silicon oxide Inorganic materials 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The invention relates to a magnetic bead transfer device and nucleic acid extraction equipment comprising the same. The magnetic bead transfer device comprises a substrate, a magnetic sleeve frame, a magnetic rod mechanism and a moving mechanism, wherein the substrate is provided with a substrate drive part; the magnetic sleeve frame is arranged on the substrate and is provided with a hollow magnetic sleeve with an opening in one end; the magnetic rod mechanism is provided with a magnetic rod with magnetism; the magnetic rod mechanism is arranged on the substrate and can carry out reciprocating motion relative to the magnetic sleeve frame under the drive of the substrate drive part, so as to drive the magnetic rod to carry out reciprocating motion relative to the magnetic sleeve frame to extend into or be far away from the magnetic sleeve; the moving mechanism is provided with a movable moving arm; and the moving arm is connected with the substrate to drive the substrate to move. According to the magnetic bead transfer device, the operation of manually adsorbing and transferring a magnetic bead can be omitted, the stability is good, the magnetic bead transfer efficiency is greatly improved, so that the nucleic acid extraction efficiency and quality are significantly improved, and the device is simple in structure and low in cost and has a good application prospect.
Description
Technical field
The present invention relates to biotechnological device field, more particularly to a kind of magnetic bead transfer device and including magnetic bead transfer
The nucleic acid extraction instrument of device.
Background technology
In recent years, with the high speed development of molecular biology, large quantities of molecular biosciences based on nucleic acid have been emerged
Experiment, such as secondary sequencing, quantitative fluorescent PCR, gene chip, making nucleic acid molecular hybridization are learned, and the integrity of DNA, RNA degrade
Degree, the purity of nucleic acid and concentration etc. all produce more or less impact to molecular biology experiment.In addition precisely medical treatment is included
Including, especially emergency treatment application (such as cause of disease quick diagnosis technology) is just being obtained more and more the medical applications based on nucleic acid
Pay attention to and apply, strong influence traditional medical diagnostic method, improve quick diagnosis level.
Method for extracting nucleic acid main at present is paramagnetic particle method, foundation and pellosil centrifugal column identical principle, with nanometer
Technology the surface of superparamagnetic nano particle is improved and surface modification after, be prepared into superparamagnetism silicon oxide nano magnetic
Pearl, the magnetic bead can specifically be recognized and efficiently combined on micro interface with nucleic acid molecules.Using silicon oxide Nano microsphere
Superparamagnetism, in the presence of guanidine hydrochloride, guanidinium isothiocyanate etc. and externally-applied magnetic field, can be from blood, animal tissue, food, cause of disease
Nucleic acid is isolated in microorganism equal samples.
Paramagnetic particle method nucleic acid extraction can be generally divided into cracking, combination, washing and eluting, however, by manually carrying out
Magnetic bead transfer operation needs to expend long time, affects the efficiency of nucleic acid extraction, and nucleic acid easily to degrade, and extraction time is long
The quality of extracted nucleic acid can be affected.
The content of the invention
Based on this, it is necessary to for it is above-mentioned manually carry out magnetic bead transfer operation expend the time it is long, nucleic acid extraction efficiency
A kind of low problem, there is provided simple efficient magnetic bead transfer device.
Specific technical scheme is as follows：
A kind of magnetic bead transfer device, including：
Substrate, the substrate is provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with hollow and one end open magnetosheath；
Bar magnet mechanism, the bar magnet mechanism is provided with the magnetic bar magnet of tool, and the bar magnet mechanism is installed on the substrate
And relatively described magnetosheath frame can move back and forth under the driving of the substrate actuator, to drive the relatively described magnetosheath of the bar magnet
Frame is moved back and forth and stretched into or away from the magnetosheath；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are moved with driving the substrate
It is dynamic.
When above-mentioned magnetic bead transfer device is used, can first place a sample in reacting hole, destroy thin by lysate, with reference to liquid
Born of the same parents' structure, makes the abundant separate out of nucleic acid, is combined with the magnetic bead in solution, then drives substrate by the transfer arm of travel mechanism
Movement makes magnetosheath stretch in reacting hole, then drives bar magnet mechanism kinematic bar magnet is stretched in magnetosheath by substrate actuator, magnetic bead
And the nucleic acid combined with magnetic bead then adsorbs on magnetosheath.Then in the reacting hole that moving substrate extend into magnetosheath for washing,
Bar magnet mechanism kinematic is driven to make bar magnet away from magnetosheath by substrate actuator, dynamic respons container makes magnetic bead and nucleic acid depart from magnetosheath
In being dispersed in cleaning mixture, and residual impurity is removed by cleaning mixture washing.Then substrate actuator drives again bar magnet mechanism to transport
Dynamic to make bar magnet stretch into magnetosheath absorption magnetic bead and nucleic acid, then moving substrate stretches into magnetosheath in the reacting hole for eluting, passes through
Nucleic acid of the eluent Dissociative on magnetic bead, last moving substrate moves away the magnetic bead that eluting is finished, high concentration and high-purity
The nucleic acid of degree is then stayed in reacting hole.By the magnetic bead transfer device, the operation of manually absorption migration magnetic bead is eliminated, surely
Qualitative good, magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and the apparatus structure letter
Single, low cost has a good application prospect.
Wherein in one embodiment, the substrate is provided with guide rail slide block, and the guide rail slide block is described including being fixed on
The guide rail of substrate and can be connected to be installed on the base with the slide block along the slide block of the slide, the bar magnet mechanism
Plate, the substrate actuator is connected to drive the bar magnet mechanism to move back and forth along the guide rail with the bar magnet mechanism.
Wherein in one embodiment, the bar magnet mechanism includes connecting plate and bar magnet frame, described connecting plate one end and institute
Slide block connection is stated, is connected with the bar magnet frame away from the other end of the slide block, the bar magnet is located at the bar magnet frame.
Wherein in one embodiment, the substrate actuator is provided with substrate drive shaft, and the substrate actuator passes through
The substrate drive shaft is threadedly coupled with the connecting plate, and the substrate actuator can drive the substrate drive shaft to rotate with band
Move the connecting plate to move back and forth along the guide rail.
Wherein in one embodiment, the bar magnet frame is provided with peace in magnetic part mounting groove, the magnetic part mounting groove
Equipped with magnetic part, the bar magnet is contacted with the magnetic part.
Wherein in one embodiment, the bar magnet mechanism is provided with bar magnet location sensitive plate, the substrate be provided with
The first position induction pieces and second position induction pieces of the substrate actuator communication connection, the bar magnet mechanism moves back and forth pre-
If apart from when, the bar magnet location sensitive plate is corresponding with the first position induction pieces or the second position induction pieces position,
So as to the first position induction pieces or the second position induction pieces control the substrate actuator stop, it is described so as to limit
The reciprocating distance of bar magnet mechanism.
Wherein in one embodiment, the magnetic part mounting groove two ends are provided with magnetic part fixed plate, and the magnetic part is consolidated
Fixed board abuts that the magnetic part is fixed in the magnetic part mounting groove with the magnetic part.
Wherein in one embodiment, the quantity of the bar magnet is multiple, a pair of multiple magnetosheaths and the bar magnet 1
Should.
Wherein in one embodiment, a diameter of 2mm-10mm of the magnetosheath.
Another object of the present invention is to a kind of nucleic acid extraction instrument is provided, including above-mentioned magnetic bead transfer device.
Description of the drawings
Fig. 1 is the magnetic bead transfer device bar magnet of an embodiment away from structural representation during magnetosheath；
Fig. 2 stretches into structural representation during magnetosheath for the magnetic bead transfer device bar magnet shown in Fig. 1.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But, the present invention can be realized in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being installed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connection
To another element or may be simultaneously present centering elements.When an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technology used herein and scientific terminology and the technical field for belonging to the present invention
The implication that technical staff is generally understood that is identical.The term for being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that of the invention in limiting.Term as used herein "and/or" includes one or more phases
The arbitrary and all of combination of the Listed Items of pass.
As depicted in figs. 1 and 2, a kind of magnetic bead transfer device 300 of a preferred embodiment of the present invention, including substrate 310, magnetic
Stock 320, bar magnet mechanism 330 and travel mechanism's (not shown).
Substrate 310 is provided with substrate actuator 311, and magnetosheath frame 320 is installed on substrate 310, and magnetosheath frame 320 is provided with hollow
And the magnetosheath 321 of one end open.Bar magnet mechanism 330 is provided with the magnetic bar magnet 331 of tool, and bar magnet mechanism 330 is installed on substrate
310 and relative magnetosheath frame 320 can move back and forth under the driving of substrate actuator 311, to drive the relative magnetosheath frame of bar magnet 331
320 move back and forth, so that bar magnet 331 is stretched into or away from magnetosheath 321.Travel mechanism has moveable transfer arm 341, mobile
Arm 341 is connected to drive substrate 310 to move with substrate 310.Specifically in the present embodiment, transfer arm 341 can drive the edge of substrate 310
The axial direction of bar magnet 331 or radial direction are moved, it will be understood that move mode not limited to this, can be according to the transfer need of magnetic bead
It is adjusted.
When the magnetic bead transfer device 300 of the present embodiment is used, in can first placing a sample into the reacting hole of Sptting plate 200, lead to
Cross lysate, destroy cellularity with reference to liquid, make the abundant separate out of nucleic acid, combined with the magnetic bead in solution, then by moving
The transfer arm 341 of motivation structure drives the movement of substrate 310 magnetosheath 321 is stretched in reacting hole, then is driven by substrate actuator 311
The motion of bar magnet mechanism 330 makes bar magnet 331 stretch in magnetosheath 321, and magnetic bead and the nucleic acid combined with magnetic bead then adsorb in magnetosheath 321
On.Then in the reacting hole that moving substrate 310 extend into magnetosheath 321 for washing, bar magnet is driven by substrate actuator 311
The motion of mechanism 330 makes bar magnet 331 away from magnetosheath 321, and dynamic respons plate 200 makes magnetic bead and nucleic acid disengaging magnetosheath 321 be dispersed in and wash
In washing liquid, and residual impurity is removed by cleaning mixture washing.Then substrate actuator 311 drives again bar magnet mechanism 330 to move
Bar magnet 331 is set to stretch into the absorption magnetic bead of magnetosheath 321 and nucleic acid, then magnetosheath 321 is stretched into moving substrate 310 reaction for eluting
Kong Zhong, by nucleic acid of the eluent Dissociative on magnetic bead, last moving substrate 310 moves away the magnetic bead that eluting is finished,
High concentration and highly purified nucleic acid are then stayed in reacting hole.By the magnetic bead transfer device 300, eliminate manually absorption and turn
The operation of magnetic bead is moved, good stability, magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality,
And the apparatus structure is simple, low cost has a good application prospect.
Specifically, substrate 310 is provided with guide rail slide block 312, and guide rail slide block 312 includes being fixed on the guide rail of substrate 310
3121 and the slide block 3122 that can slide along guide rail 3121, bar magnet mechanism 330 be connected to be installed on substrate 310, base with slide block 3122
Plate actuator 311 is connected to drive bar magnet mechanism 330 to move back and forth along guide rail 3121 with bar magnet mechanism 330.In the present embodiment,
Substrate actuator 311 is linear stepping motor, is fixed on substrate 310 by substrate actuator bracing frame 3111.
Specifically, bar magnet mechanism 330 includes connecting plate 332 and bar magnet frame 333, and the one end of connecting plate 332 connects with slide block 3122
Connect, be connected with bar magnet frame 333 away from the other end of slide block 3122, bar magnet 331 is located at bar magnet frame 333.
Further, substrate actuator 311 is provided with substrate drive shaft 3112, and substrate actuator 311 is driven by substrate
Axle 3112 is threadedly coupled with connecting plate 332, substrate actuator 311 the substrate drive shaft 311 can be driven to rotate in different directions with
Connecting plate 332 is driven to move back and forth along guide rail 3121.
Preferably, bar magnet frame 333 is provided with magnetic part mounting groove 3331, magnetic part mounting groove 3331 and is provided with magnetic part
3332, bar magnet 331 is contacted with magnetic part 3332, and so as to pass through magnetic part 3332 magnetic of bar magnet 331 is strengthened, and makes magnetic bead energy of adsorption
Power is more preferable.
Preferably, bar magnet mechanism 330 is provided with bar magnet location sensitive plate 334, and substrate 310 is provided with and substrate actuator
The first position induction pieces 313 and second position induction pieces 314 of 311 communication connections, bar magnet mechanism 330 moves back and forth predeterminable range
When, bar magnet location sensitive plate 334 is corresponding with first position induction pieces 313 or the position of second position induction pieces 314, so that first
Put induction pieces 313 or the control base board actuator 311 of second position induction pieces 314 stops, back and forth transporting so as to limit bar magnet mechanism 330
Dynamic distance, makes bar magnet 331 control more stable with the relative distance of magnetosheath 321 and accurate.In the present embodiment, substrate 310
On be fixed with circuit board 315, first position induction pieces 313 and second position induction pieces 314 are optocoupler, are installed on circuit board 315,
With sensing groove, bar magnet mechanism 330 moves back and forth can be such that bar magnet location sensitive plate 334 stretches in corresponding sensing groove, so that the
One location sensitive part 313 or the control base board actuator 311 of second position induction pieces 314 stop, and prevent bar magnet mechanism 330 from continuing original
The motion in direction.
Preferably, the two ends of magnetic part mounting groove 3331 are provided with magnetic part fixed plate 3333, magnetic part fixed plate 3333 and magnetic
Property part 3332 abuts that magnetic part 3332 is fixed in magnetic part mounting groove 3331, so as to keep stablizing for magnetic part 3332.
In the present embodiment, the quantity of bar magnet 331 is 12, and 12 magnetosheaths 321 are corresponded with bar magnet 331, and bar magnet 331 is in
Linear array, it is corresponding with the reacting hole of 96 orifice plates.It is appreciated that the quantity and arrangement mode of bar magnet 331 can be as needed
Adjustment, to adapt to different reaction vessel and demand.
Further, a diameter of 2mm-10mm of magnetosheath 321, the sample volume for extracting is minimum up to 20uL, energy
Enough save sample and cost.
The magnetic bead transfer device 300 of the present embodiment can save the operation of manually absorption migration magnetic bead, good stability, magnetic
Pearl transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and the apparatus structure is simple, low cost,
Have a good application prospect.
As shown in figure 1, the nucleic acid extraction instrument (not shown) of a preferred embodiment of the present invention, including above-mentioned magnetic bead transfer dress
Put 300.The nucleic acid extraction instrument of the present invention eliminates the behaviour of manually absorption migration magnetic bead by magnetic bead transfer device 300
Make, good stability, magnetic bead transfer efficiency is greatly improved, thus significantly improves nucleic acid extraction efficiency and quality, and the device knot
Structure is simple, and low cost has a good application prospect.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more concrete and detailed, but and
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that for one of ordinary skill in the art comes
Say, without departing from the inventive concept of the premise, some deformations and improvement can also be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (10)
1. a kind of magnetic bead transfer device, it is characterised in that include：
Substrate, the substrate is provided with substrate actuator；
Magnetosheath frame, is installed on the substrate, and the magnetosheath frame is provided with hollow and one end open magnetosheath；
Bar magnet mechanism, the bar magnet mechanism is provided with the magnetic bar magnet of tool, and the bar magnet mechanism is installed on the substrate and can
Relatively described magnetosheath frame is moved back and forth under the driving of the substrate actuator, to drive the relatively described magnetosheath frame of the bar magnet past
Move again and stretch into or away from the magnetosheath；
Travel mechanism, with moveable transfer arm, the transfer arm and the substrate connection are moved with driving the substrate.
2. magnetic bead transfer device according to claim 1, it is characterised in that the substrate is provided with guide rail slide block, described
Guide rail slide block include be fixed on the substrate guide rail and can be along the slide block of the slide, the bar magnet mechanism and the cunning
Block connects to be installed on the substrate, and the substrate actuator is connected to drive the bar magnet mechanism along institute with the bar magnet mechanism
State guide rail reciprocating motion.
3. magnetic bead transfer device according to claim 2, it is characterised in that the bar magnet mechanism includes connecting plate and bar magnet
Frame, described connecting plate one end is connected with the slide block, is connected with the bar magnet frame away from the other end of the slide block, the bar magnet
Located at the bar magnet frame.
4. magnetic bead transfer device according to claim 3, it is characterised in that the substrate actuator is provided with substrate driving
Axle, the substrate actuator is threadedly coupled by the substrate drive shaft with the connecting plate, and the substrate actuator can drive
The substrate drive shaft rotates to drive the connecting plate to move back and forth along the guide rail.
5. magnetic bead transfer device according to claim 3, it is characterised in that the bar magnet frame is provided with magnetic part installation
Groove, is provided with magnetic part in the magnetic part mounting groove, the bar magnet is contacted with the magnetic part.
6. magnetic bead transfer device according to claim 1, it is characterised in that the bar magnet mechanism is provided with the sense of bar magnet position
Plate, the substrate is answered to be provided with first position induction pieces and second position induction pieces with substrate actuator communication connection,
When the bar magnet mechanism moves back and forth predeterminable range, the bar magnet location sensitive plate and the first position induction pieces or described the
Two location sensitive part positions correspondence, so as to the first position induction pieces or the second position induction pieces control the substrate drive
Moving part stops, so as to limit the reciprocating distance of the bar magnet mechanism.
7. magnetic bead transfer device according to claim 5, it is characterised in that the magnetic part mounting groove two ends are provided with magnetic
Part fixed plate, the magnetic part fixed plate abuts for the magnetic part to be fixed on the magnetic part mounting groove with the magnetic part
It is interior.
8. magnetic bead transfer device according to claim 1, it is characterised in that the quantity of the bar magnet is multiple, Duo Gesuo
State magnetosheath to correspond with the bar magnet.
9. the magnetic bead transfer device according to any one of claim 1-8, it is characterised in that a diameter of 2mm- of the magnetosheath
10mm。
10. a kind of nucleic acid extraction instrument, it is characterised in that including the magnetic bead transfer device described in any one of claim 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710026417.9A CN106675991A (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and nucleic acid extraction equipment comprising same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710026417.9A CN106675991A (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and nucleic acid extraction equipment comprising same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106675991A true CN106675991A (en) | 2017-05-17 |
Family
ID=58858960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710026417.9A Pending CN106675991A (en) | 2017-01-13 | 2017-01-13 | Magnetic bead transfer device and nucleic acid extraction equipment comprising same |
Country Status (1)
| Country | Link |
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| CN (1) | CN106675991A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107118958A (en) * | 2017-07-13 | 2017-09-01 | 上海奇法生物仪器有限公司 | Nucleic acid purifier |
| CN107988045A (en) * | 2018-01-08 | 2018-05-04 | 深圳市朗司医疗科技有限公司 | A kind of full-automatic nucleic acid purification system based on paramagnetic particle method |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108277148A (en) * | 2018-04-25 | 2018-07-13 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid distributing device |
| CN108642045A (en) * | 2018-05-08 | 2018-10-12 | 绍兴迅敏康生物科技有限公司 | A kind of bar magnet set |
| CN109280663A (en) * | 2018-09-30 | 2019-01-29 | 成都博奥独立医学实验室有限公司 | A kind of full-automatic single nucleic acid strands preparation method |
| CN110511865A (en) * | 2019-08-29 | 2019-11-29 | 中国科学院苏州生物医学工程技术研究所 | High-throughput nucleic acid extraction system |
| CN113866430A (en) * | 2021-09-17 | 2021-12-31 | 北京知新上智科技有限公司 | Magnetic bead adding device, blood coagulation equipment and magnetic bead adding method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107118958A (en) * | 2017-07-13 | 2017-09-01 | 上海奇法生物仪器有限公司 | Nucleic acid purifier |
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| CN107988045B (en) * | 2018-01-08 | 2024-01-19 | 深圳市朗司医疗科技有限公司 | Full-automatic nucleic acid purification system based on magnetic bead method |
| CN108102912A (en) * | 2018-03-05 | 2018-06-01 | 江苏硕世生物科技股份有限公司 | A kind of nucleic acid extraction instrument and system |
| CN108277148A (en) * | 2018-04-25 | 2018-07-13 | 江苏硕世生物科技股份有限公司 | Nucleic acid extraction liquid distributing device |
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| CN109280663A (en) * | 2018-09-30 | 2019-01-29 | 成都博奥独立医学实验室有限公司 | A kind of full-automatic single nucleic acid strands preparation method |
| CN110511865A (en) * | 2019-08-29 | 2019-11-29 | 中国科学院苏州生物医学工程技术研究所 | High-throughput nucleic acid extraction system |
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