CN108277148A - Nucleic acid extraction liquid distributing device - Google Patents
Nucleic acid extraction liquid distributing device Download PDFInfo
- Publication number
- CN108277148A CN108277148A CN201810383680.8A CN201810383680A CN108277148A CN 108277148 A CN108277148 A CN 108277148A CN 201810383680 A CN201810383680 A CN 201810383680A CN 108277148 A CN108277148 A CN 108277148A
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- CN
- China
- Prior art keywords
- extraction
- plate
- nucleic acid
- driving portion
- magnetosheath
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The present invention provides a kind of nucleic acid extraction liquid distributing device, belongs to nucleic acid extraction technical field.Extraction plate is slideably positioned in the surface of pedestal, extracts and is provided with multiple drawing holes on plate, extraction unit is for extracting nucleic acid solution to drawing holes.Liquid separation portion includes mounting plate and liquid separatnig plate, and mounting plate and liquid separatnig plate may be contained within the surface of pedestal, and multiple liquid separation holes are provided on liquid separatnig plate；It is provided with multiple mounting holes on mounting plate, transfer member is provided in mounting hole.Mounting bracket is set to pedestal, transfer sets up and is placed in mounting bracket, first driving portion is set to mounting bracket and is drivingly connected with transfer frame, for driving transfer frame in mounting plate and extraction plate and the upper direction of liquid separatnig plate, second driving portion is set to transfer frame and is drivingly connected with installation part, for driving installation part to move up and down and the transfer member being made selectively to be installed on installation part.This nucleic acid extraction liquid distributing device can realize the extraction and liquid separation of nucleic acid, convenient to be subsequently detected to nucleic acid.
Description
Technical field
The present invention relates to nucleic acid extraction technical fields, in particular to a kind of nucleic acid extraction liquid distributing device.
Background technology
Method for extracting nucleic acid generally comprise cell cracking, enzymatic treatment, nucleic acid detached with other biological macromolecular substances, nucleic acid
Several key steps such as purifying, and each step can alone or in combination be realized by a variety of different methods.
Instrument for extracting nucleic acid is the instrument that the extraction work of sample nucleic acid is automatically performed using mating nucleic acid extracting reagent.
The method of generally use centrifugal clarification during traditional manual nucleic acid extraction, when operation, are time-consuming and laborious.In recent years, occur
Beads enrichment technology, nucleic acid extraction are extracted based on paramagnetic particle method, and the cell pyrolysis liquid in paramagnetic particle method is a kind of protein denaturant, can
So that the cell cracking of animals and plants, and making the protein denaturation combined with DNA, the free releases of DNA, magnetic bead can be adsorbed specifically
DNA removes the impurity such as protein, the polysaccharide other than DNA, then the DNA with eluent Dissociative on magnetic bead by washing,
The very high DNA of purity and concentration is obtained, PCR masterplates, genetic engineering etc. are can be used for.
The instrument for extracting nucleic acid of the prior art can not achieve the process of automatic liquid separation, operate complex, inefficiency.
Invention content
The purpose of the present invention is to provide a kind of nucleic acid extraction liquid distributing devices, can realize the extraction and liquid separation of nucleic acid, carry
High working efficiency.
Realization that the present invention adopts the following technical solutions：
A kind of nucleic acid extraction liquid distributing device, including：
Pedestal；
Extraction assembly, extraction assembly include at least one extraction element, and each extraction element includes extraction unit and extraction plate,
Extraction plate is slideably positioned in the surface of pedestal, extracts and is provided with multiple drawing holes on plate, extraction unit be used for by nucleic acid solution extract to
In drawing holes；
Liquid separation portion, liquid separation portion include mounting plate and liquid separatnig plate, and mounting plate and liquid separatnig plate may be contained within the surface of pedestal, liquid separation
Multiple liquid separation holes are provided on plate；It is provided with multiple mounting holes on mounting plate, transfer member is provided in mounting hole；And
Transfer assembly, transfer assembly include transfer frame, mounting bracket, the first driving portion, the second driving portion and transfer member, installation
Erection is placed in pedestal, and transfer, which is set up, is placed in mounting bracket, and the first driving portion is set to mounting bracket and is drivingly connected with transfer frame, is used for
Driving transfer frame mounting plate with extraction plate and liquid separatnig plate upper direction, the second driving portion be set to transfer frame and with installation
Part is drivingly connected, for driving installation part to move up and down and transfer member being made selectively to be installed on installation part.
Further, in preferred embodiments of the present invention, above-mentioned transfer assembly further includes third driving portion, and mounting bracket includes
First montant, the second montant and cross bar；
First montant and the second montant are slideably positioned in pedestal, and cross bar is set on the first montant and the second montant, turn
It moves to set up and is placed in cross bar, third driving portion is drivingly connected in the first montant and the second montant, for driving mounting bracket to slide.
Further, in preferred embodiments of the present invention, said extracted component includes the first extraction element and the second extraction
Device；
The extraction plate of first extraction element and the extraction plate of the second extraction element are respectively arranged at the both ends of pedestal, and third is driven
Dynamic portion is for driving mounting bracket to be slided between the extraction plate and the extraction plate of the second extraction element of the first extraction element.
Further, in preferred embodiments of the present invention, said extracted portion includes fourth drive part, the 5th driving portion, more
Root magnetosheath and Duo Gen bar magnets；
Fourth drive part and the 5th driving portion are set to pedestal, and every magnetosheath and bar magnet are respectively positioned on the top of extraction plate, and one
Root bar magnet is slideably positioned in a magnetosheath, and fourth drive part is drivingly connected in bar magnet for driving bar magnet to be transported up and down in magnetosheath
Dynamic, the 5th driving portion is drivingly connected in magnetosheath for driving magnetosheath to move up and down in drawing holes.
Further, in preferred embodiments of the present invention, multiple rows of drawing holes is set, in two rows of drawing holes on said extracted plate
It is provided with magnetic bead, more magnetosheaths are two rows of magnetosheaths corresponding with magnetic bead, and more bar magnets are two rows of bar magnets corresponding with magnetosheath.
Further, in preferred embodiments of the present invention, above-mentioned each extraction element further includes the 6th driving portion, and the 6th drives
Dynamic portion is drivingly connected extraction plate for driving extraction plate to be moved to the direction of another row's magnetosheath along row's magnetosheath.
Further, in preferred embodiments of the present invention, said extracted device further includes bogie, and fourth drive part includes
4th motor, the 4th lead screw, the 4th nut and the 4th frame；
Bogie is set to pedestal, and the 4th motor is set on bogie, and the output shaft of the 4th motor is drivingly connected the 4th
Lead screw, the 4th nut are sheathed on outside the 4th lead screw, and the 4th frame is fixedly connected on the 4th nut, and four gimbaled is located at the 4th
Fixed two rows bar magnet on the frame plate of bar both sides.
Further, in preferred embodiments of the present invention, said extracted portion further includes occlusion part, and occlusion part is set to multiple rows of
Drawing holes, for separating magnetosheath and drawing holes.
Further, in preferred embodiments of the present invention, above-mentioned occlusion part includes the 7th driving portion, L-shaped shielding plate and screening
Divider；
It blocks erection and is placed in bogie, the 7th driving portion, which is slideably positioned in, blocks frame, and one end of L-shaped shielding plate is driven with the 7th
Dynamic portion's connection, the other end is set to multiple rows of drawing holes, for separating magnetosheath and drawing holes.
Further, in preferred embodiments of the present invention, above-mentioned nucleic acid extraction liquid distributing device further includes control system, and first
Driving portion, the second driving portion, fourth drive part, the 5th driving portion and the 7th driving portion are electrically connected with control system.
The advantageous effect of nucleic acid extraction liquid distributing device that presently preferred embodiments of the present invention provides is：Extraction unit is first passed through by sample
Nucleic acid extraction in product enters in drawing holes, and the second driving portion driving transfer frame moves downward, and the transfer member in mounting hole is made to pacify
It feed into installation part, the second driving portion driving transfer frame moves upwards, and the driving of the first driving portion shifts frame from the upper of mounting plate
Side is moved to the top of extraction plate, and the second driving portion driving transfer frame moves downward, and transfer member is made to enter the extraction equipped with nucleic acid
In hole, transfer frame is driven to move upwards by nucleic acid inspiration transfer member, then by the second motor；Continue that the first driving portion is made to drive
Transfer frame is moved to liquid separatnig plate from extraction plate, and the second driving portion driving transfer frame declines, and the nucleic acid in transfer member is moved to point
In fluid apertures, to carry out the detection of nucleic acid.
Description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings and also belong to protection scope of the present invention.
Fig. 1 is the structural schematic diagram of nucleic acid extraction liquid distributing device provided in an embodiment of the present invention；
Fig. 2 is the structural schematic diagram that plate is extracted in nucleic acid extraction liquid distributing device provided in an embodiment of the present invention；
Fig. 3 is the structural schematic diagram of pedestal in nucleic acid extraction liquid distributing device provided in an embodiment of the present invention；
Fig. 4 is the first enlarged drawing at IV in Fig. 1；
Fig. 5 is the enlarged drawing at V in Fig. 3；
Fig. 6 is the second enlarged drawing at IV in Fig. 1；
Fig. 7 is the structural schematic diagram of transfer assembly in nucleic acid extraction liquid distributing device provided in an embodiment of the present invention.
Icon：100- pedestals；200- extraction assemblies；300- transfer assembly；400- liquid separations portion；500- first is extracted
Device；The second extraction elements of 600-；210- extraction units；220- extracts plate；221- drawing holes；222- first rows；223-
Second row；224- thirds are arranged；The 4th rows of 225-；The 5th rows of 226-；The 6th rows of 227-；The 7th rows of 228-；229- the 8th
Row；The 9th rows of 231-；The tenth rows of 232-；The 11st rows of 233-；The 12nd rows of 234-；The 6th driving portions of 240-；241-
6th motor；The 6th lead screws of 242-；The 6th nuts of 243-；244- sliding slots；211- fourth drive parts；212- the 5th drives
Portion；213- bar magnets；214- magnetosheaths；The 4th motors of 251-；The 4th lead screws of 252-；The 4th nuts of 253-；The 4th frames of 254-
Frame；The 5th motors of 261-；The 5th lead screws of 262-；The 5th nuts of 263-；The 5th frames of 264-；270- occlusion parts；271-
7th driving portion；272-L shape shielding plates；273- blocks frame；274- connectors；275- shutters；410- mounting plates；
420- liquid separatnig plates；430- mixed plates；440- agent plates；310- shifts frame；320- mounting brackets；The first driving portions of 330-；
The second driving portions of 340-.
Specific implementation mode
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention
In attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is
A part of the embodiment of the present invention, instead of all the embodiments.The present invention being usually described and illustrated herein in the accompanying drawings is implemented
The component of example can be arranged and be designed with a variety of different configurations.Therefore, below to the reality of the present invention provided in the accompanying drawings
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
It should be noted that：Similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi
It is defined, then it further need not be defined and explained in subsequent attached drawing in a attached drawing.
Embodiment
Fig. 1 is the structural schematic diagram of nucleic acid extraction liquid distributing device provided in this embodiment.Referring to Fig. 1, in the present embodiment,
Nucleic acid extraction liquid distributing device includes pedestal 100, extraction assembly 200, transfer assembly 300 and liquid separation portion 400, and pedestal 100 is nucleic acid
The installation foundation of the other component of extraction element, extraction assembly 200, transfer assembly 300 and liquid separation portion 400 are all disposed within pedestal
On 100.
Pedestal 100 is one piece of bottom plate, and base plate interior is provided with slot, for installing extraction plate 220, transfer assembly 300 and dividing
Liquid portion 400.In order to do more multisample, in the present embodiment, extraction assembly 200 includes at least one extraction element, Mei Geti
It includes extraction unit 210 and extraction plate 220 to take device.Optionally, extraction assembly 200 includes two extraction elements, two extraction dresses
It sets and is relatively arranged on pedestal 100.Extraction assembly 200 includes the first extraction element 500 and the second extraction element 600；First extraction
The extraction plate 220 of device 500 and the extraction plate 220 of the second extraction element 600 are respectively arranged at the both ends of pedestal 100.
An extraction plate 220 is arranged in each extraction element, then two extraction plates 220, two extraction plates are arranged on pedestal 100
220 structure is identical, and each plate 220 that extracts is slideably positioned in pedestal 100, and each extract is respectively provided with multiple extractions on plate 220
Hole 221 is selectively provided with magnetic bead in drawing holes 221, and extraction unit 210 is for extracting nucleic acid solution to drawing holes 221.
Fig. 2 is the structural schematic diagram that plate 220 is extracted in nucleic acid extraction liquid distributing device provided in this embodiment.Also referring to
Fig. 1 and Fig. 2 in the present embodiment, extracts and multiple rows of drawing holes 221 is arranged on plate 220, magnetic is provided at least row's drawing holes 221
Pearl.It in order to disposably extract more samples, extracts and multiple rows of drawing holes 221 is set on plate 220, in two rows of drawing holes 221
It is provided with magnetic bead.
In the present embodiment, extracts and be provided with 12 row's drawing holes 221 altogether on plate 220, wherein 222 row of first row and the 7th
Lysate is housed, for lysed sample, the drawing holes 221 of second row 223 and the 8th row 229 are built-in in the drawing holes 221 of row 228
There are magnetic bead, third to arrange the drawing holes of the 224, the 4th row 225, the 5th row 226, the 9th row 231, the tenth row 232, the 11st row 233
Different cleaning solutions is housed, for removing the impurity such as the protein in sample, polysaccharide, 227 row of the 6th row and the 12nd row in 221
Eluent is housed, for the nucleic acid samples on magnetic bead to be eluted into eluent in 234 drawing holes 221.
Fig. 3 is the structural schematic diagram of pedestal 100 in nucleic acid extraction liquid distributing device provided in this embodiment.Fig. 3 is pedestal 100
Structure schematic diagram, please refer to Fig.1 and Fig. 3, each extraction element further include the 6th driving portion 240, the 6th driving portion 240
It is set to pedestal 100, drives extraction plate 220 to be slided on 100 surface of pedestal by the 6th driving device.
6th driving portion 240 includes the 6th motor 241, the 6th lead screw 242, the 6th nut 243, the setting of the 6th motor 241
In in pedestal 100,100 surface of pedestal is provided with sliding slot 244, and the output shaft of the 6th motor 241 is drivingly connected the 6th lead screw 242,
6th nut 243 is sheathed on outside the 6th lead screw 242, extracts the sliding part that sliding slot 244 is provided through on 220 lower face of plate and the
Six nuts 243 are fixedly connected.
When 6th motor 241 rotates, the 6th lead screw 242 rotates together, since the restriction of sliding slot 244 and sliding part is made
With the 6th nut 243 being sheathed on outside the 6th lead screw 242 cannot be rotated with the 6th lead screw 242, and the 6th nut 243 occurs directly
Line moves, to make the extraction plate 220 being connect with the 6th nut 243 by sliding part be slided on 100 surface of pedestal.Pass through control
6th motor 241 rotates and reverse, and to make extraction plate 220 slide in the opposite direction, i.e., is turned by the 6th motor 241
It is dynamic, so that extraction plate 220 is moved back and forth along X-direction.
240 phase of the 6th driving portion of the 6th driving portion 240 and the second extraction element 600 of two the first extraction elements 500
To setting, 220 relative motion of extraction plate or the phase of the extraction plate 220 and the second extraction element 600 of the first extraction element 500 of driving
Counter-movement.
Fig. 4 is the first enlarged drawing at IV in Fig. 1；Fig. 5 is the enlarged drawing at V in Fig. 3.Also referring to Fig. 1-Fig. 5,
In the present embodiment, extraction unit 210 includes fourth drive part 211, the 5th driving portion 212, more magnetosheaths 214 and Duo Gen bar magnets 213,
Fourth drive part 211 and the 5th driving portion 212 are set to pedestal 100, and every magnetosheath 214 and bar magnet 213 are respectively positioned on extraction plate 220
Top, a bar magnet 213 is slideably positioned in a magnetosheath 214, and the drive connection of fourth drive part 211 is used in bar magnet 213
Bar magnet 213 is driven to move up and down in magnetosheath 214, the 5th driving portion 212 is drivingly connected in magnetosheath 214 for driving magnetosheath 214
It moves up and down in drawing holes 221.
Bar magnet 213 is set to move up and down by fourth drive part 211, bar magnet 213 is moved downwardly in magnetosheath 214, is transported upwards
It is dynamic to stretch out magnetosheath 214；Magnetosheath 214 is set to move up and down by the 5th driving portion 212, magnetosheath 214 moves downwardly into drawing holes 221
Interior, magnetosheath 214 moves upwards, and stretches out drawing holes 221.
Bar magnet 213 is coaxially disposed with magnetosheath 214, so that a bar magnet 213 is slideably positioned in a magnetosheath 214, more
Magnetosheath 214 is at least row magnetosheath 214 corresponding with magnetic bead, and more bar magnets 213 are at least row bar magnet corresponding with magnetosheath 214
213, i.e. more magnetosheaths 214 form an at least row, and more bar magnets 213 form an at least row.
More magnetosheaths 214 are two rows of magnetosheaths 214 corresponding with magnetic bead, and more bar magnets 213 are two rows of corresponding with magnetosheath 214
Bar magnet 213, i.e. more magnetosheaths 214 form two rows of magnetosheaths 214, and more bar magnets 213 form two rows of bar magnets 213, when row's magnetosheath 214
When corresponding with the drawing holes 221 of the 12nd row 234, correspondingly, the drawing holes 221 of 214 and the 6th row 227 of another row's magnetosheath
It is corresponding.When row's magnetosheath 214 is corresponding with 223 row's drawing holes 221 of second row, correspondingly, another row's magnetosheath 214 and the 8th
229 row's drawing holes 221 correspondence is arranged, and so on.
6th driving portion 240 is drivingly connected extraction plate 220, for driving extraction plate 220 along row's magnetosheath 214 to another row
The direction of magnetosheath 214 moves.I.e. by the effect of the 6th motor 241, the 6th lead screw 242 and the 6th nut 243, make extraction plate
220 reciprocatingly slide in 244 upper edge of sliding slot, one row's magnetosheath 214 to the direction of another row's magnetosheath 214, i.e., are moved back and forth along X-direction.
Extraction element further includes bogie, and bogie is for installing fourth drive part 211 and the 5th driving portion 212.4th
Driving portion 211 includes the 4th motor 251, the 4th lead screw 252, the 4th nut 253 and the 4th frame 254；Bogie is set to bottom
Seat 100, the 4th motor 251 is set on bogie, and the output shaft of the 4th motor 251 is drivingly connected the 4th lead screw 252, the 4th spiral shell
Mother 253 is sheathed on outside the 4th lead screw 252, and the 4th frame 254 is fixedly connected on the 4th nut 253, and the 4th frame 254 is located at the
Fixed two rows bar magnet 213 on the frame plate of four lead screws, 252 both sides.
4th lead screw 252 is vertically arranged, and correspondingly, bar magnet 213 is also vertically arranged.When 4th motor 251 rotates, the
Four lead screws 252 rotate together, due to the restriction effect of the 4th frame 254 and bar magnet 213, be sheathed on outside the 4th lead screw 252 the
Four nuts 253 cannot be rotated with the 4th lead screw 252, to which the 4th nut 253 moves up and down, make to connect with the 4th nut 253
The 4th frame 254 move up and down, then the bar magnet 213 being fixed on the 4th frame 254 also moves up and down.Pass through the 4th electricity of control
Machine 251 rotates and reverse, and to make bar magnet 213 move up and down, i.e., is moved back and forth along Z-direction.
In the present embodiment, the 4th frame 254 includes the first frame being oppositely arranged and the second frame and is oppositely arranged
Third frame and the 4th frame, third frame are connected to the 4th nut 253, the first frame and the second frame of the 4th frame 254
Upper to fix row's bar magnet 213 respectively, bar magnet 213 moves up and down to synchronize with the 4th frame 254 and move up and down, i.e., past along Z-direction
Multiple movement.
5th driving portion 212 includes the 5th motor 261, the 5th lead screw 262, the 5th nut 263 and the 5th frame 264；The
Five motors 261 are set on bogie, and the output shaft of the 5th motor 261 is drivingly connected the 5th lead screw 262, the 5th 263 sets of nut
Outside the 5th lead screw 262, the 5th frame 264 is fixedly connected on the 5th nut 263, and the 5th frame 264 is located at the 5th lead screw
Fixed two rows magnetosheath 214 on the frame plate of 262 both sides.
5th lead screw 262 is vertically arranged, and correspondingly, magnetosheath 214 is also vertically arranged.When 5th motor 261 rotates, the
Five lead screws 262 rotate together, due to the restriction effect of the 5th frame 264 and magnetosheath 214, be sheathed on outside the 5th lead screw 262 the
Five nuts 263 cannot be rotated with the 5th lead screw 262, to which the 5th nut 263 moves up and down, make to connect with the 5th nut 263
The 5th frame 264 move up and down, then the magnetosheath 214 being fixed on the 5th frame 264 also moves up and down.Pass through the 5th electricity of control
Machine 261 rotates and reverse, and to make magnetosheath 214 move up and down, i.e., is moved back and forth along Z-direction.
In the present embodiment, the 5th frame 264 includes the first frame being oppositely arranged and the second frame and is oppositely arranged
Third frame and the 4th frame, third frame are connected to the 5th nut 263, the first frame and the second frame of the 5th frame 264
Upper to fix row's magnetosheath 214 respectively, magnetosheath 214 moves up and down to synchronize with the 5th frame 264 and move up and down, i.e., past along Z-direction
Multiple movement.
Fig. 6 is the second enlarged drawing at IV in Fig. 1.Also referring to Fig. 4 and Fig. 6, extraction unit 210 further includes occlusion part
270, occlusion part 270 is set to multiple rows of drawing holes 221, for separating magnetosheath 214 and drawing holes 221.After extraction is completed,
Mobile extraction plate 220, in order to avoid magnetic bead surfaces on magnetosheath 214 sample drop into drawing holes 221, passing through occlusion part 270 will
Magnetic bead is separated with the drawing holes 221 equipped with eluent on magnetosheath 214, avoids the sample in eluent contaminated.
Occlusion part 270 includes the 7th driving portion 271, L-shaped shielding plate 272 and blocks frame 273.Frame 273 is blocked to be set to
Bogie, the 7th driving portion 271, which is slideably positioned in, blocks frame 273, and one end that L-shaped shielding plate is 272 connects with the 7th driving portion 271
It connects, the other end is set to drawing holes 221, for separating magnetosheath 214 and drawing holes 221.
By the 7th driving portion 271, control L-shaped shielding plate 272 is arranged between two rows of magnetosheaths 214 or row's drawing holes
221 top, if extraction plate 220 is moved from first row 222 towards the direction of the drawing holes 221 of the 12nd row 234, hide
Catch is arranged above the drawing holes 221 of the 6th row 227.If extracting plate 220 from the 12nd row 234 towards first row 222
The direction of drawing holes 221 moves, then, shielding plate is arranged above the drawing holes 221 of the 12nd row 234, avoids in eluent
Sample is contaminated.
7th driving portion 271 includes the 7th motor, the 7th lead screw, the 7th nut；7th motor, which is set to, blocks frame 273,
The output shaft of 7th motor is drivingly connected the 7th lead screw, and the 7th nut is sheathed on outside the 7th lead screw and connects with the sliding of frame 273 is blocked
It connects, one end that L-shaped shielding plate is 272 is fixedly connected in the 7th nut.
7th lead screw is laterally arranged, and when the 7th motor rotates, the 7th lead screw rotates together, due to blocking frame 273
Restriction effect, the 7th nut being sheathed on outside the 7th lead screw cannot be rotated with the 7th lead screw, to which the 7th nut is blocking frame
Horizontal sliding on 273 makes the horizontal sliding on blocking frame 273 of the L-shaped shielding plate being connect with the 7th nut 272.Pass through control
7th motor rotates and reverse, to make L-shaped shielding plate 272 move back and forth in the horizontal direction, i.e., reciprocal along X-direction
Movement.
L-shaped shielding plate 272 includes connector 274 and shutter 275, and connector 274 forms L-shaped knot with shutter 275
Structure, one end of connector 274 is connect with the 7th nut and the extending direction one of the extending direction of connector 274 and the 7th lead screw
It causes, the extending direction of shutter 275 is vertical with the extending direction of the 7th lead screw, the extending direction of shutter 275 and row's magnetosheath
214 orientation is consistent, to separate magnetosheath 214 and drawing holes 221.
After nucleic acid extraction completion, nucleic acid solution is subsequently subjected to liquid separation for convenience, is needed the first extraction element 500
Extraction plate 220 and the extraction plate 220 of the second extraction element 600 be moved to the middle part of pedestal 100, even if the first extraction element
500 extraction plate 220 and the extraction unit 210 of the first extraction element 500 is staggered, the extraction plate 220 of the second extraction element 600 and
The extraction unit 210 of two extraction elements 600 is staggered, when extracting the movement of plate 220, in order to avoid the liquid on magnetosheath 214 falls
Enter contaminated nucleic acid liquid in drawing holes 221, then needs to control the 7th motor, shutter 275 is made to be moved to the lower section of magnetosheath 214, it will
Magnetosheath 214 is separated with drawing holes 221, and the nucleic acid solution on magnetosheath 214 is avoided to fall into drawing holes 221 in pollution drawing holes 221
Nucleic acid solution.
Optionally, when extraction plate 220 is moved to the middle part of pedestal 100, shutter 275 is arranged in the first extraction element
500 and second extraction element 600 outside the lower section of magnetosheath 214, avoid the nucleic acid solution in drawing holes 221 from polluting.
Liquid separation portion 400 includes mounting plate 410 and liquid separatnig plate 420, and mounting plate 410 and liquid separatnig plate 420 may be contained within pedestal 100
Surface, be provided with multiple liquid separation holes on liquid separatnig plate 420；It is provided with multiple mounting holes on mounting plate 410, is provided in mounting hole
Transfer member.
Mounting plate 410 is mainly used to that installation part is arranged, and there are one mounting plates 410, and multiple installations are provided on mounting plate 410
Hole is provided with transfer member in mounting hole.In the present embodiment, transfer member is tip heads, and tip, for drawing nucleic acid solution, can control
Draw the amount of nucleic acid solution.
Liquid separation portion 400 further includes mixed plate 430 and agent plate 440, and multiple mix apertures, agent plate is arranged in mixed plate 430
It is provided with multiple reagent wells on 440, reagent bottle can be inserted in reagent wells, the reagent drawn in reagent bottle by tip will try
Agent is transferred in mix aperture, then nucleic acid solution is put into mix aperture, so that nucleic acid solution and reagent is mixed, is obtained sample to be detected.
Liquid separatnig plate 420 is that pcr plates are dropped to by tip on pcr plates after mixing nucleic acid solution and reagent, then by pcr
Plate is removed, and the detection that nucleic acid is carried out on pcr instruments is placed on.In the present embodiment, liquid separatnig plate 420 includes three pcr plates, pcr plates
Number can be determined according to the quantity of sample.
Transfer assembly is that extraction assembly 200 is extracted obtained nucleic acid solution to be transferred to the mix aperture being arranged on mixed plate 430
It is interior, then the agent transfer in reagent wells after so that reagent is mixed with nucleic acid solution, is transferred on liquid separatnig plate 420 into mix aperture
Liquid separation hole in, obtain sample to be tested.
Fig. 7 is the structural schematic diagram of transfer assembly in nucleic acid extraction liquid distributing device provided in this embodiment.Also referring to
Fig. 1 and Fig. 7, in the present embodiment, transfer assembly includes transfer frame 310, mounting bracket 320, the first driving portion 330, the second driving portion
340 and installation part, mounting bracket 320 is set to pedestal 100, and transfer frame 310 is set to mounting bracket 320, the setting of the first driving portion 330
In mounting bracket 320 and with transfer frame 310 be drivingly connected, for drive transfer frame 310 mounting plate 410 with extraction plate 220 and
The upper direction of liquid separatnig plate 420, the second driving portion 340 is set to transfer frame 310 and is drivingly connected with installation part, for driving peace
Piece installing moves up and down and transfer member is made selectively to be installed on installation part.
Mounting bracket 320 includes the first montant, the second montant and cross bar；First montant and the second montant are slideably positioned in bottom
Seat 100, cross bar is set on the first montant and the second montant, and transfer frame 310 is set to cross bar.Transfer assembly further includes that third is driven
Dynamic portion, third driving portion is for driving mounting bracket 320 in the extraction plate 220 and the second extraction element 600 of the first extraction element 500
Extraction plate 220 between slide.I.e. third driving portion is drivingly connected in the first montant and the second montant, for driving mounting bracket
320 slidings, i.e., third driving portion is for driving the first montant and the second montant to be slided on pedestal 100, to make to be fixed on cross
Transfer frame 310 on bar is sliding between extraction plate 220 and the extraction plate 220 of the second extraction element 600 of the first extraction element 500
It is dynamic.I.e. third driving portion can be such that the first montant of mounting bracket 320 and the second montant is slided on pedestal 100, the first montant and
Second montant is back and forth transported from 220 direction of extraction plate of extraction plate 220 towards the second extraction element 600 of the first extraction element 500
It is dynamic, i.e., it is moved back and forth along X-direction.
Third driving portion is set in pedestal 100, and third driving portion includes two, and each third driving portion includes third electricity
The output shaft of machine, third lead screw and third nut, third motor is drivingly connected third lead screw, and third motor and third lead screw position
In in pedestal 100, third nut is sheathed on outside third lead screw, and the first montant is connected to the third nut of a third driving portion,
Second montant is connected to the third nut of another third driving portion, and the third motor of two third driving portions moves synchronously, from
And the first montant and the second montant is made to move synchronously, achieve the purpose that the position that movable stand is moved in X-direction.
First driving portion 330 is set to the cross bar of mounting bracket 320, and the first driving portion 330 is drivingly connected with transfer frame 310,
For driving transfer frame 310 to reciprocatingly slide on cross bar, i.e., moved in the direction of cross bar upper edge Y-axis, the first driving portion 330 includes
The output shaft of first motor, the first lead screw, the first nut, first motor is drivingly connected the first lead screw, the extension side of the first lead screw
To consistent with the extending direction of cross bar, the first nut is sheathed on outside the first lead screw, and the first nut is slideably positioned in cross bar, shifts frame
310 are fixed on the first nut, and transfer frame 310 is moved synchronously with the first nut.
Second driving portion 340 includes the second motor, the second lead screw and the second nut, and the second motor is set to transfer frame 310,
The output shaft of second motor is drivingly connected the second lead screw, and the second nut is sheathed on outside the second lead screw, and installation part is fixed on the second spiral shell
Mother, the second lead screw is vertically arranged, and when the second motor rotates, the second nut moves up and down, i.e., is moved along Z-direction, with the
The installation part of two nuts connection also moves up and down, and when installation part moves to lower section on tip heads, installation part grips tip,
So that tip settings on mountings, then by first motor and third motor is shifted, until arrival purpose, then passes through
Liquid in tip is squeezed out, retransfers position, draw liquid again by tip absorption liquid, transferring position, and so on.
Nucleic acid extraction liquid distributing device further includes control system, the first driving portion 330, the second driving portion 340, fourth drive part
211, the 5th driving portion 212 and the 7th driving portion 271 are electrically connected with control system.
Further, control system and first motor, the second motor, third motor, the 4th motor 251, the 5th motor
261, the 6th motor 241 and the 7th motor are electrically connected, and control system sends out signal, and by the rotating forward of signal control motor with
Reversion, to realize the extraction and liquid separation of nucleic acid.
The operation principle of nucleic acid extraction liquid distributing device provided in this embodiment is：In carrying for first row 222 and the 7th row 228
221 built-in lysate of hole is taken, in the 221 built-in magnetic bead of drawing holes of second row 223 and the 8th row 229, third arranges the 224, the 4th row
225, different cleaning solutions is housed in the drawing holes 221 of the 5th row 226, the 9th row 231, the tenth row 232, the 11st row 233, the
Eluent is housed in the drawing holes 221 of six rows 227 and the 12nd row 234；Reagent wells interpolation Reagent Tube in agent plate 440, peace
Mounting hole interpolation tip heads in loading board 410.
First sample is placed in the drawing holes 221 equipped with lysate, the rotation of the 5th motor 261 makes magnetosheath 214 along Z axis
It moves up and down, shakes, beats the mixed liquor of lysate and sample, keep sample broken, nucleic acid enters in lysate.
The 6th motor 241 is controlled, sliding panel is made to be slided along X-axis, makes 214 face of two rows of magnetosheaths that the second row of magnetic bead to be housed
223 and 229 drawing holes 221 of the 8th row, bar magnet 213 and magnetosheath 214 are controlled together by the 4th motor 251 and the 5th motor 261
It is moved downward along Z axis, makes bar magnet 213 will be in 223 drawing holes 221 of second row and 229 drawing holes 221 of the 8th row across magnetosheath 214
Magnetic bead attract get up.
The 6th motor 241 is controlled, sliding panel is made to be slided along X-axis, makes 214 face of two rows of magnetosheaths that the first row of sample to be housed
222 and 228 drawing holes 221 of the 7th row, the rotation of the 4th motor 251 makes bar magnet 213 be moved upwards along Z axis, the rotation of the 5th motor 261
So that magnetosheath 214 is moved downward along Z axis, the magnetic bead of attraction is made to enter the drawing holes of first row 222 and the 7th row 228 equipped with sample
In 221, continues through the 5th motor 261 control magnetosheath 214 and move up and down along Z axis, make its concussion, to make sample be sticked to magnetic bead
Surface controls bar magnet 213 and magnetosheath 214 by the 4th motor 251 and the 5th motor 261 and is moved downward together along Z axis, made together
Bar magnet 213 attracts the magnetic bead for being stained with sample across magnetosheath 214.
The 6th motor 241 is controlled, extraction plate 220 is slided along X-axis, makes to have attracted the 214 face third of magnetosheath of magnetic bead to arrange 224
With 221 top of drawing holes of the 9th row 231, the rotation of the 4th motor 251 makes bar magnet 213 be moved upwards along Z axis, the 5th motor 261
Rotation makes magnetosheath 214 be moved downward along Z axis, and the magnetic bead of attraction is made to enter the third row's 224 and the 9th row 231 that cleaning solution is housed
In drawing holes 221, the 5th motor 261 controls magnetosheath 214 and moves up and down repeatedly along Z axis, and protein, polysaccharide in sample etc. is miscellaneous
Matter removes, and leaves nucleic acid absorption on the surface of magnetic bead, bar magnet is controlled together by fourth drive part 211 and the 5th driving portion 212
213 and magnetosheath 214 moved downward together along Z axis, make bar magnet 213 across magnetosheath 214 will be stained with sample magnetic bead attract, lead to
It crosses this mode and washs sample in the 4th row 225 and the tenth row 232, the 5th row 226 and the 11st row 233 again successively, finally by sample
The impurity such as protein, polysaccharide in this all remove.
The 6th motor 241 is controlled, extraction plate 220 is slided along X-axis, makes 214 face of magnetosheath for having attracted magnetic bead equipped with elution
6th row 227 of liquid and 221 top of drawing holes of the 12nd row 234, the rotation of the 4th motor 251 make bar magnet 213 be transported upwards along Z axis
Dynamic, the rotation of the 5th motor 261 makes magnetosheath 214 be moved downward along Z axis, and the magnetic bead of attraction is made to enter the drawing holes equipped with eluent
In 221, nucleic acid solution is obtained in eluent to make the nucleic acid on magnetic bead enter, takes out magnetic bead afterwards.
The rotation of the 7th motor is controlled, so that L-shaped shielding plate 272 is moved along the direction of X-axis, the screening of the first extraction element 500
Baffle 275 blocks the outside magnetosheath 214 of the first extraction element 500, and the shutter 275 of the second extraction element 600 blocks second and carries
The outside magnetosheath 214 of device 600, control the 6th motor 241 rotation is taken to make the extraction plate 220 and second of the first extraction element 500
The extraction plate 220 of extraction element 600 slides in opposition along X-axis, and two extraction plates 220 is made to be located at the middle part of pedestal 100.Also,
During two extraction plates 220 slide, due to the effect of blocking of shutter 275, the nucleic acid solution on magnetosheath 214 is avoided to enter
In drawing holes 221 equipped with eluent, the pollution of sample is avoided.
The rotation of the second motor is controlled, installation part is moved downward along Z axis, and the heads of the tip in mounting hole is made to be fit into installation part
Interior, the second motor reversal makes installation part be moved upwards along Z axis, and third motor drives the first montant and the second montant along X-direction
Movement makes transfer frame 310 be located at identical X-axis direction of displacement with agent plate 440, and first motor control transfer frame 310 is along Y-axis side
To sliding, transfer frame 310 is made to be moved to the top of agent plate 440, control the second motor rotation, installation part is moved downward along Z axis,
The tip heads in installation part are made to draw the reagent in reagent bottle, the second motor reversal makes installation part be moved upwards along Z axis.Continue to control
The rotation of third motor and first motor processed makes transfer frame 310 be moved to the top of mixed plate 430, controls the second motor, makes
Reagent in tip is squeezed into mix aperture, and the tip heads on installation part are removed.
Being continuing with aforesaid way takes new tip heads, the rotation of third motor that transfer frame 310 is made to be located at phase with extraction plate 220
Same X-axis direction of displacement, first motor control transfer frame 310 are slided along Y direction, and transfer frame 310 is made to be moved to extraction plate 220
Top, control the second motor rotation, installation part moves downward along Z axis, makes in the tip heads absorption drawing holes 221 in mounting hole
Nucleic acid solution, the second motor reversal makes installation part be moved upwards along Z axis.Continue the rotation of control third motor and first motor,
So that transfer frame 310 is moved to the top of mixed plate 430, control the second motor, the nucleic acid solution in tip is made to squeeze into mix aperture,
Nucleic acid solution is mixed with reagent in mix aperture.
It is continuing with aforesaid way and takes new tip heads, control the rotation of third motor, first motor and the second motor, make
The tip mixed liquors drawn in mix aperture are transferred in the liquid separation hole of pcr plates, obtain sample to be tested, subsequently to use pcr instrument
Device carries out the detection of sample.
This nucleic acid extraction liquid distributing device realizes that nucleic acid automatically extracts the behaviour of purifying and PCR PCR system constructions
Make, instead of artificial, liberation manpower；Simultaneously reduce the biohazard that operating personnel face, can safely, fast and accurately, stablize,
Low-cost realization PCR detects pre-treatment, and PCR detections can be directly carried out after being disposed.PCR detection method is clinically fast
Fast diagnosing bacterial infectious disease etc. has particularly important meaning.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of nucleic acid extraction liquid distributing device, which is characterized in that including：
Pedestal；
Extraction assembly, the extraction assembly include at least one extraction element, and each extraction element includes extraction unit and carries
Plate, the extraction plate is taken to be slideably positioned in the surface of the pedestal, multiple drawing holes, the extraction are provided on the extraction plate
Portion is for extracting nucleic acid solution to the drawing holes；
Liquid separation portion, the liquid separation portion include mounting plate and liquid separatnig plate, and the mounting plate and the liquid separatnig plate may be contained within the bottom
The surface of seat is provided with multiple liquid separation holes on the liquid separatnig plate；Multiple mounting holes, the mounting hole are provided on the mounting plate
Inside it is provided with transfer member；And
Transfer assembly, the transfer assembly includes shifting frame, mounting bracket, the first driving portion, the second driving portion and transfer member, described
Mounting bracket is set to the pedestal, and the transfer erection is placed in the mounting bracket, and first driving portion is set to the installation
Frame and with the transfer frame be drivingly connected, for driving the transfer frame in the mounting plate and the extraction plate and described point
The upper direction of liquid plate, second driving portion is set to the transfer frame and is drivingly connected with the installation part, for driving
The installation part moves up and down and the transfer member is made selectively to be installed on the installation part.
2. nucleic acid extraction liquid distributing device according to claim 1, which is characterized in that the transfer assembly further includes that third is driven
Dynamic portion, the mounting bracket include the first montant, the second montant and cross bar；
First montant and second montant are slideably positioned in the pedestal, and the cross bar is set to first montant
On second montant, the transfer erection is placed in the cross bar, and the third driving portion is drivingly connected perpendicular in described first
Bar and second montant, for driving the mounting bracket to slide.
3. nucleic acid extraction liquid distributing device according to claim 2, which is characterized in that the extraction assembly includes the first extraction
Device and the second extraction element；
The extraction plate of first extraction element and the extraction plate of second extraction element are respectively arranged at described
The both ends of pedestal, the third driving portion is for driving the mounting bracket in the extraction plate of first extraction element and institute
It states and slides between the extraction plate of the second extraction element.
4. according to claim 1-3 any one of them nucleic acid extraction liquid distributing devices, which is characterized in that the extraction unit includes
Fourth drive part, the 5th driving portion, more magnetosheaths and Duo Gen bar magnets；
The fourth drive part and the 5th driving portion are set to the pedestal, and the every magnetosheath and the bar magnet are respectively positioned on
The top of the extraction plate, a bar magnet are slideably positioned in a magnetosheath, and the fourth drive part is drivingly connected
In the bar magnet for driving the bar magnet to move up and down in the magnetosheath, the 5th driving portion is drivingly connected in the magnetic
Set is for driving the magnetosheath to move up and down in the drawing holes.
5. nucleic acid extraction liquid distributing device according to claim 4, which is characterized in that be arranged on the extraction plate multiple rows of described
Drawing holes, is provided with magnetic bead in two rows of drawing holes, and the more magnetosheaths are two rows of magnetosheaths corresponding with the magnetic bead, more
The bar magnet is two rows of bar magnets corresponding with the magnetosheath.
6. nucleic acid extraction liquid distributing device according to claim 5, which is characterized in that each extraction element further includes the
Six driving portions, the 6th driving portion are drivingly connected the extraction plate and are used to drive the extraction plate along magnetosheath described in a row to another
The direction of magnetosheath described in one row moves.
7. nucleic acid extraction liquid distributing device according to claim 6, which is characterized in that the extraction element further includes driving
Frame, the fourth drive part include the 4th motor, the 4th lead screw, the 4th nut and the 4th frame；
The bogie is set to the pedestal, and the 4th motor is set on the bogie, the 4th motor it is defeated
Shaft is drivingly connected the 4th lead screw, and the 4th nut is sheathed on outside the 4th lead screw, and the 4th frame, which is fixed, to be connected
It is connected to the 4th nut, four gimbaled two rows of magnetic fixed on the frame plate of the 4th lead screw both sides
Stick.
8. nucleic acid extraction liquid distributing device according to claim 7, which is characterized in that the extraction unit further includes occlusion part,
The occlusion part is set to multiple rows of drawing holes, for separating the magnetosheath and the drawing holes.
9. nucleic acid extraction liquid distributing device according to claim 8, which is characterized in that the occlusion part includes the 7th driving
Portion, L-shaped shielding plate and block frame；
It is described to block erection and be placed in the bogie, the 7th driving portion be slideably positioned in it is described block frame, the L-shaped is blocked
One end of piece is connect with the 7th driving portion, and the other end is set to multiple rows of drawing holes, for by the magnetosheath with it is described
Drawing holes separates.
10. nucleic acid extraction liquid distributing device according to claim 9, which is characterized in that the nucleic acid extraction liquid distributing device is also
Including control system, first driving portion, second driving portion, the fourth drive part, the 5th driving portion and institute
The 7th driving portion is stated to be electrically connected with the control system.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810383680.8A CN108277148A (en) | 2018-04-25 | 2018-04-25 | Nucleic acid extraction liquid distributing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810383680.8A CN108277148A (en) | 2018-04-25 | 2018-04-25 | Nucleic acid extraction liquid distributing device |
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| CN108277148A true CN108277148A (en) | 2018-07-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113801770A (en) * | 2021-10-11 | 2021-12-17 | 谢央达 | Pretreatment device for nucleic acid detection |
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