CN113801770B - Nucleic acid detection pretreatment device - Google Patents
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Abstract
The application provides a nucleic acid detection pretreatment device, which comprises a sterile isolation box body, an extraction plate, a cup separation treatment mechanism and a nucleic acid extraction mechanism, wherein the extraction plate is arranged in the sterile isolation box body; the cup separating processing mechanism is arranged in the sterile isolation box body and is used for separating the samples conveyed into the sterile isolation box body into cups and samples on the extraction plate; the nucleic acid extraction mechanism is arranged in the sterile isolation box body and is used for extracting nucleic acid from the sample on the extraction plate. The device can reduce the infection risk and the protection level requirement on detection personnel, and improves the treatment efficiency.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a nucleic acid detection pretreatment device.
Background
Nucleic acid testing is the finding of the presence or absence of foreign invading viral nucleic acid in a patient's respiratory specimen, blood or stool to determine if it is infected with a virus. Thus, once detected as "positive" for nucleic acid, the presence of virus in the patient is demonstrated.
After obtaining the detection sample, the operations of cup separation and sample separation, nucleic acid extraction, amplification plate liquid separation and sample separation and the like are generally required to be carried out, but the current operation flows are respectively carried out by adopting independent devices, specifically, after the detection sample is separated into cups in a cup separation processing system, the detection sample is transferred into a nucleic acid extractor by a detection personnel for nucleic acid extraction, and after the nucleic acid extraction is finished, the detection sample is transferred into an amplification plate liquid separation and sample separation device by the detection personnel for amplification plate liquid separation and sample separation. Therefore, the whole nucleic acid detection pretreatment process needs actual contact operation of detection personnel, the infection risk is high, the protection level requirement on the detection personnel is high, and the treatment efficiency is low.
Disclosure of Invention
The embodiment of the application aims to provide a nucleic acid detection pretreatment device which is used for reducing infection risk and protection grade requirements on detection personnel and improving treatment efficiency.
The embodiment of the application provides a pretreatment device for nucleic acid detection, which comprises a sterile isolation box body, an extraction plate, a cup separation treatment mechanism and a nucleic acid extraction mechanism, wherein the extraction plate is arranged in the sterile isolation box body; the cup separating processing mechanism is arranged in the sterile isolation box body and is used for separating the samples conveyed into the sterile isolation box body into cups and samples on the extraction plate; the nucleic acid extraction mechanism is arranged in the sterile isolation box body and is used for extracting nucleic acid from the sample on the extraction plate.
In the implementation mode, the aseptic isolation box body is a highly clean and continuous effective operation space brought by adopting an aseptic isolation technology, and can furthest reduce the pollution of microorganisms and various particles, thereby improving the accuracy of aseptic inspection. According to the application, two operations before nucleic acid detection, namely cup separation and sample separation processing and nucleic acid extraction are integrated in the sterile isolation box, a sample is separated into samples in the sterile isolation box through the cup separation processing mechanism and then the samples on the extraction plate are subjected to nucleic acid extraction through the nucleic acid extraction mechanism, the cup separation and sample separation operations and the nucleic acid extraction operations are sequentially completed in the sterile isolation box, the extraction plate is not required to be manually transferred into the nucleic acid extraction instrument for nucleic acid extraction after the cup separation and sample separation, i.e. the manual operation between the cup separation and sample separation processing and the nucleic acid extraction is omitted, the chance that a detector directly contacts the samples is reduced, and the sterile isolation box is matched, so that the infection risk is effectively reduced, the protection level requirements on the detector are further reduced, and meanwhile, the processing efficiency is improved.
In one possible implementation manner, the nucleic acid detection pretreatment device further comprises an amplification plate and a liquid and sample separation mechanism, wherein the amplification plate is arranged in the sterile isolation box; the liquid separating and sampling mechanism is arranged in the sterile isolation box body and is used for separating the samples extracted by the nucleic acid on the extraction plate into the amplification plate.
In the implementation mode, the three operations before nucleic acid extraction, namely cup separation and sample separation, nucleic acid extraction and liquid separation and sample separation are integrated in the sterile isolation box body, so that manual transfer among the three operations can be omitted, and further the infection risk is effectively reduced.
In one possible implementation manner, the aseptic isolation box comprises a cup separating treatment bin, an extraction liquid separating bin and a transfer channel, wherein the cup separating treatment bin is internally provided with the cup separating treatment mechanism and the extraction plate; the nucleic acid extraction mechanism, the amplification plate and the liquid and sample separation mechanism are arranged in the extraction and liquid separation bin, and the air pressure of the extraction and liquid separation bin is larger than the air pressure in the cup separation treatment bin; the transfer channel is arranged between the cup separation treatment bin and the extraction liquid separation bin and is used for communicating the cup separation treatment bin and the extraction liquid separation bin. The nucleic acid detection pretreatment device further comprises an extraction plate transfer mechanism, wherein the extraction plate transfer mechanism is arranged in the sterile isolation box and used for transferring the extraction plate carrying the sample from the cup separation treatment bin to the extraction and liquid separation bin through the transfer channel.
In the implementation mode, an operation space in the sterile isolation box body is divided into an independent cup separation treatment bin and an extraction liquid separation bin, samples are separated into samples on an extraction plate in the cup separation treatment bin through a cup separation treatment mechanism, the extraction plate with the samples is transferred into the extraction liquid separation bin through a transfer channel by an extraction plate transfer mechanism, then a nucleic acid extraction mechanism in the extraction liquid separation bin extracts nucleic acid from the samples on the extraction plate, and the samples after the nucleic acid extraction are transferred onto an amplification plate. Because the air pressure of the extraction and separation liquid bin is greater than the air pressure in the separation cup treatment bin, when the extraction and separation liquid bin is communicated with the separation cup treatment bin through a transfer channel, namely, when an extraction plate is transferred, the air in the extraction and separation liquid bin flows into the separation cup treatment bin, so that the generated aerosol in the separation cup treatment bin can be effectively prevented from polluting the extraction and separation liquid bin, a pollution area and a semi-pollution area can be arranged in a segmented mode, and the extraction and separation liquid bin is a semi-pollution area.
In one possible implementation manner, the nucleic acid detection pretreatment device further comprises a first air inlet filter device, a second air inlet filter device and a first air outlet filter device, wherein the first air inlet filter device is arranged at one side of the bottom of the cup separation treatment bin, which is far away from the extraction liquid separation bin, and is used for filtering external air and inputting the filtered external air into the cup separation treatment bin; the second air inlet filter device is arranged at one side of the bottom of the cup separating treatment bin, which is close to the extracting and liquid separating bin, and is used for filtering external air and inputting the filtered external air into the cup separating treatment bin; the first exhaust filtering device is arranged at the top of the cup separating treatment bin and is used for filtering and discharging the gas in the cup separating treatment bin.
In the implementation mode, the first air inlet filter device and the second air inlet filter device are respectively arranged on two sides of the bottom of the cup separating treatment bin, air enters the cup separating treatment bin after being filtered by the first air inlet filter device and the second air inlet filter device, and the air in the cup separating treatment bin is discharged out of the sterile isolation box body through the first air outlet filter device, so that an air passage is formed between the first air inlet filter device and the first air outlet filter device, another air passage is formed between the second air inlet filter device and the first air outlet filter device, and further the rear end of a pollutant logistics such as virus aerosol at the front end of the cup separating treatment bin can be effectively reduced, and cross contamination is formed.
In one possible implementation manner, the nucleic acid detection pretreatment device further comprises a third air inlet filter device, a fourth air inlet filter device and a second air outlet filter device, wherein the third air inlet filter device is arranged at one side, close to the cup separation treatment bin, of the bottom of the extraction liquid separation bin and is used for filtering external gas and inputting the filtered external gas into the extraction liquid separation bin; the fourth air inlet filter device is arranged at one side of the bottom of the extraction and liquid separation bin far away from the cup separation treatment bin and is used for filtering external air and inputting the filtered external air into the extraction and liquid separation bin; the second exhaust filtering device is arranged at the top of the extraction and liquid separation bin and is used for filtering and discharging the gas in the extraction and liquid separation bin.
In the implementation mode, the third air inlet filter device and the fourth air inlet filter device are respectively arranged on two sides of the bottom of the extraction and liquid separation bin, air enters the extraction and liquid separation bin after being filtered by the third air inlet filter device and the fourth air inlet filter device, and air in the extraction and liquid separation bin is discharged out of the sterile isolation box body through the second air outlet filter device, so that an air passage is formed between the third air inlet filter device and the first air outlet filter device, another air passage is formed between the fourth air inlet filter device and the second air outlet filter device, and further the rear end of a polluted material flow such as virus aerosol at the front end of the extraction and liquid separation bin can be effectively reduced, and cross contamination is formed.
In one possible implementation manner, a first bin gate is arranged between the transfer channel and the cup separating treatment bin, and the first bin gate is used for being opened when the extraction plate enters the transfer channel and closed after the extraction plate enters the transfer channel; the transfer channel with be provided with the second door between the extraction divides the liquid storehouse, the second door is used for the extraction board get into the transfer channel just first door is closed the back and is opened, and the extraction board gets into the extraction divides the liquid storehouse back and closes. The nucleic acid detection pretreatment device further comprises a first automatic killing device, wherein the first automatic killing device is used for killing the transfer channel when the extraction plate enters the transfer channel and the first bin gate and the second bin gate are closed.
In the implementation mode, the first automatic sterilizing device and the switch between the first bin gate and the second bin gate are matched, so that pollution sources in the cup separating treatment bin can be effectively prevented from entering the extraction liquid separating bin, and the cup separating treatment bin and the extraction liquid separating bin are effectively isolated.
In one possible implementation, the air pressure within the sub-cup treatment chamber is less than the ambient air pressure.
In the implementation mode, when the sample is conveyed from the outside into the cup separating treatment bin, the air pressure in the cup separating treatment bin is smaller than the outside atmospheric pressure, and the air flow direction is from the outside into the cup separating treatment bin, so that the leakage of a pollution source such as virus aerosol in the cup separating treatment bin can be effectively prevented.
In a possible implementation manner, the aseptic isolation box further comprises a sample input channel, wherein the sample input channel is used for communicating the cup separation treatment bin with the outside, one end of the sample input channel, which is communicated with the outside, is provided with a third bin gate, and the third bin gate is used for being opened when a sample is conveyed from the outside to the sample conveying bin and is closed after the sample enters the sample conveying bin; a fourth bin gate is arranged between the sample input channel and the cup separating treatment bin, and is used for being opened after a sample enters the sample conveying bin and the third bin gate is closed, and is closed after the extraction plate enters the extraction liquid separating bin; the device also comprises a sample conveying mechanism and a second automatic killing device, wherein the sample conveying mechanism is arranged in the sample input channel or the sub-cup treatment bin and is used for conveying a sample from the sample input channel to the sub-cup treatment bin; the second automatic sterilizing device is used for sterilizing the sample conveying bin after the sample enters the sample conveying bin and the third bin gate and the fourth bin gate are closed.
In this implementation, the process starts, the third door is opened, the sample enters the sample input channel from the outside through the third door, then the third door is closed, the sample input channel is in a closed state at this time, and the second automatic sterilizing device is started to sterilize the sample input channel and the sample in the sample input channel. After the sterilization is finished, the fourth bin gate is opened, the sample is transferred into the cup separating treatment bin through the sample conveying mechanism, and finally the fourth bin gate is closed, and the air pressure in the sample input channel is the same as the air pressure in the cup separating treatment bin. When the third bin gate is opened, the air pressure in the sample input channel is the same as the air pressure in the sub-cup treatment bin, and the air pressure in the sub-cup treatment bin is smaller than the external atmospheric pressure, so that the external air flows into the sample input channel, and the leakage of virus aerosol and other pollution sources in the sample input channel can be effectively avoided.
In one possible implementation, the extraction and separation cartridge has a gas pressure less than the ambient atmospheric pressure.
In the implementation process, when the amplification plate is conveyed from the extraction and separation bin to the outside, the air pressure of the extraction and separation bin is smaller than the external atmospheric pressure, and the air flow direction is from the outside to the extraction and separation bin, so that the leakage of pollution sources such as virus aerosol in the extraction and separation bin can be effectively prevented.
In a possible implementation manner, the aseptic isolation box further comprises an amplification plate output channel for communicating the extraction and separation bin with the outside, and a fifth bin gate is arranged between the amplification plate output channel and the extraction and separation bin and is used for being opened when the amplification plate is conveyed from the extraction and separation bin to the amplification plate output channel and being closed after the amplification plate enters the amplification plate output channel; a sixth bin gate is arranged at one end, communicated with the outside, of the amplification plate output channel, and is used for being opened after the amplification plate enters the amplification plate output channel and the fifth bin gate is closed, and is closed after the amplification plate enters the outside; the nucleic acid detection pretreatment device further comprises an amplification plate output mechanism and a third automatic killing device, wherein the amplification plate output mechanism is arranged in the amplification plate output channel or the extraction and liquid separation bin and is used for moving the amplification plate from the extraction and liquid separation bin to the amplification plate output channel; the third automatic sterilizing device is used for sterilizing the amplification plate output channel after the amplification plate enters the amplification plate output channel and the fifth bin gate and the sixth bin gate are closed.
In the implementation mode, the third automatic sterilizing device is arranged on the output channel of the amplification plate and matched with the switch of the fifth bin gate and the sixth bin gate, so that the leakage of a pollution source in the extracting and separating bin can be effectively avoided.
In one possible implementation, the aseptic isolation tank further includes a first material input channel for communicating an outside with the sub-cup treatment bin; a seventh bin gate is arranged at one end, communicated with the outside, of the first material input channel, and is used for being opened when the extraction plate conveys the extraction plate from the outside into the first material input channel and closed after the extraction plate enters the first material input channel; an eighth bin gate is arranged at one end of the first material input channel, which is communicated with the cup separating treatment bin, and is used for being opened after the extraction plate enters the first material input channel and the seventh bin gate is closed, and is closed after the extraction plate enters the cup separating treatment bin; the nucleic acid detection pretreatment device further comprises a first material input mechanism and a fourth automatic killing device, wherein the first material input mechanism is arranged in the first material input channel and is used for conveying the extraction plate from the outside into the cup separation treatment bin; the fourth automatic sterilizing device is arranged in the first material input channel and is used for sterilizing the first material input channel after the extraction plate enters the first material input channel and the seventh bin gate and the eighth bin gate are closed simultaneously.
In the implementation process, the pollution sources in the cup separating treatment bin can be effectively prevented from leaking out through the first material input channel by arranging the fourth sterilizing device and the matched switches of the seventh bin gate and the eighth bin gate.
In one possible implementation process, the aseptic isolation box further comprises a second material input channel for communicating the outside with the extraction and separation bin, and a ninth bin gate is arranged at one end of the second material input channel, which is communicated with the outside, and is used for being opened when the amplification plate is conveyed into the second material input channel from the outside and being closed after the amplification plate enters the second material input channel; a tenth bin gate is arranged at one end of the second material input channel, which is communicated with the extraction and liquid separation bin, and is used for being opened after the amplification plate enters the second material input channel and the ninth bin gate is closed, and is closed after the amplification plate enters the extraction and liquid separation bin; the device also comprises a second material input mechanism and a fifth killing device, wherein the second material input mechanism is arranged in the second material input channel and is used for conveying the amplification plate from the outside into the extraction and liquid separation bin; the fifth sterilizing device is arranged in the second material input channel and is used for sterilizing the second material input channel.
In the implementation process, the application can effectively prevent the pollution source in the extracting and separating liquid bin from leaking out through the second material input channel by arranging the fifth sterilizing device and the matched switches of the ninth bin gate and the tenth bin gate.
In one possible implementation process, the aseptic isolation box body further comprises a first waste output channel, one end of the first waste output channel is communicated with the cup separation treatment bin, and the other end of the first waste output channel is arranged on the outer surface of the aseptic isolation box; the nucleic acid detection pretreatment device further comprises a first waste output pipe, one end of the first waste output pipe is communicated with the first waste output channel, and the other end of the first waste output pipe extends to a first preset position.
In the implementation process, the waste in the cup separation treatment bin reaches the first waste output pipe through the first waste output channel, and then is guided out to the first preset position through the first waste output pipe, optionally, the first preset position is located outside the laboratory where the device is located before the nucleic acid detection treatment, so that the pollution level of the laboratory where the device is located before the nucleic acid detection treatment can be reduced, and the protection level of detection personnel is further reduced.
In one possible implementation process, the aseptic isolation box body further comprises a second waste output channel, one end of the second waste output channel is communicated with the extraction and liquid separation bin, and the other end of the second waste output channel is arranged on the outer surface of the aseptic isolation box; the nucleic acid detection pretreatment device further comprises a second waste output pipe, one end of the second waste output pipe is communicated with the second waste output channel, and the other end of the second waste output pipe extends to a second preset position.
In the implementation process, the waste in the extraction and liquid separation bin reaches the second waste output pipe through the second waste output channel, and then is guided out to a second preset position through the second waste output pipe, optionally, the second preset position is located outside a laboratory where the device is located before the nucleic acid detection processing, and then the pollution level of the laboratory where the device is located before the nucleic acid detection processing can be reduced, so that the protection level of detection personnel is reduced.
The application has the beneficial effects that: the application builds the whole shell frame through the aseptic isolation box body, and integrates the cup separation and sample separation, nucleic acid extraction and liquid separation and sample separation into the aseptic isolation box body to form an integrated treatment system. The inner part of the aseptic isolation box body is provided with a pollution area and a semi-pollution area, and the pressure intensity is adjusted by adopting a gas filtering device, so that the internal cross pollution of the cup separation treatment bin and the extraction and liquid separation bin is avoided. The aseptic isolation box body is internally provided with a sample input channel, an amplification plate output channel, a material input channel and a waste output channel which are respectively provided with an automatic sterilizing device. The automatic cup separation processing mechanism and the nucleic acid extraction mechanism are utilized to realize full automation of cup separation and sample separation, nucleic acid extraction and liquid separation and sample separation, and the accuracy of detection data can be improved. Therefore, the whole process of the nucleic acid detection pretreatment is operated in a non-contact manner in the closed sterile isolation box body, the problem of high risk of infection caused by manual contact operation of detection personnel can be effectively avoided, the protection level requirement on the detection personnel is reduced, and the three-level protection can be reduced to the two-level protection by adopting the nucleic acid detection pretreatment device. The nucleic acid detection pretreatment device has high detection flux, and compared with the treatment mode in the prior art, the treatment efficiency is effectively improved, and the labor intensity of detection personnel is reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments of the present application will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and should not be considered as limiting the scope, and other related drawings can be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block diagram of a nucleic acid detecting pretreatment apparatus according to an embodiment of the present application;
FIG. 2 is a view showing a structure of a nucleic acid detecting pretreatment device shown in FIG. 1 from another perspective;
FIG. 3 is a schematic diagram showing a top view of the nucleic acid detecting pretreatment apparatus shown in FIG. 1.
Icon: 100-a sterile isolation box; 200-extraction plate; 300-a cup separating treatment mechanism; 400-nucleic acid extraction means; 500-amplification plates; 600-liquid and sample separating mechanism; 110-separating cup treatment bins; 120-extracting and separating the liquid bin; 130-transit pass; 700-an extraction plate transfer mechanism; 131-a first bin gate; 132-a second bin gate; 140-sample input channel; 800-sample delivery mechanism; 141-a third bin gate; 142-fourth bin gate; 150-amplification plate output channel; 151-fifth bin gate; 152-sixth bin gate; 900-amplification plate output mechanism; 160-a first material input channel; 161-seventh bin gate; 162-eighth bin gate; 170-a second material input channel; 171-ninth bin gate; 172-tenth bin gate; 180-a first waste output channel; 190-a second waste output channel.
Detailed Description
The technical solutions in the embodiments of the present application will be described below with reference to the accompanying drawings in the embodiments of the present application.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures. Meanwhile, in the description of the present application, the terms "first", "second", and the like are used only to distinguish the description, and are not to be construed as indicating or implying relative importance.
In a first aspect, referring to fig. 1, 2 and 3, fig. 1 is a block diagram of a nucleic acid pretreatment device according to an embodiment of the present application, fig. 2 is a block diagram of another view angle of the nucleic acid pretreatment device shown in fig. 1, and fig. 3 is a block diagram of a top view angle of the nucleic acid pretreatment device shown in fig. 1, the device includes a sterile isolation box 100, an extraction plate 200, a cup separation processing mechanism 300, and a nucleic acid extraction mechanism 400, the extraction plate 200 being disposed in the sterile isolation box 100; the cup separating processing mechanism 300 is arranged in the aseptic isolation box 100 and is used for separating the samples conveyed into the aseptic isolation box 100 into cups and samples on the extraction plate 200; the nucleic acid extraction mechanism 400 is disposed within the sterile isolation housing 100 for nucleic acid extraction of a sample on the extraction plate 200.
In this implementation, the aseptic isolation enclosure 100 is a highly clean, continuous and efficient operating space that is provided by aseptic isolation techniques, which minimizes contamination by microorganisms and various particulates, thereby improving the accuracy of the aseptic inspection. According to the application, two operations before nucleic acid detection, namely cup separation and sample separation processing and nucleic acid extraction are integrated in the sterile isolation box 100, a sample is separated into samples in the sterile isolation box 100 through the cup separation processing mechanism 300 and then the samples on the extraction plate 200 are subjected to nucleic acid extraction through the nucleic acid extraction mechanism 400, the cup separation and sample separation operations and the nucleic acid extraction operations are sequentially completed in the sterile isolation box 100, the extraction plate 200 is not required to be manually transferred into a nucleic acid extractor for nucleic acid extraction after cup separation and sample separation, namely, the manual operation between cup separation and sample separation processing and nucleic acid extraction is omitted, the probability of direct contact of detection personnel with the samples is reduced, and in addition, the sterile isolation box 100 is matched, so that the infection risk is effectively reduced, the protection level requirements on the detection personnel are further reduced, and meanwhile, the processing efficiency is improved.
Optionally, a transparent visual window is provided on the sterile isolation housing 100 to allow a inspector to observe the operating conditions inside the sterile isolation housing 100.
In one possible implementation manner, the nucleic acid detection pretreatment device further comprises an amplification plate 500 and a liquid and sample separation mechanism 600, wherein the amplification plate 500 is arranged in the sterile isolation box 100; the liquid-separating and sample-separating mechanism 600 is disposed in the aseptic isolation cabinet 100, and is used for separating the nucleic acid-extracted sample on the extraction plate 200 to the amplification plate 500.
In this embodiment, the liquid-separating and sample-separating mechanism 600, the cup-separating processing mechanism 300 and the nucleic acid extracting mechanism 400 are integrated in the aseptic processing box, and the cup-separating and sample-separating, the nucleic acid extracting and the liquid-separating of the sample are sequentially completed in the aseptic isolation box 100, and the nucleic acid-extracted sample on the liquid-separating amplification plate 500 can be directly subjected to nucleic acid detection. Therefore, the three operations before nucleic acid extraction, namely cup separation and sample separation, nucleic acid extraction and liquid separation and sample separation are integrated in the sterile isolation box 100, so that manual transfer among the three operations can be omitted, and further the infection risk is effectively reduced.
In one possible implementation, the aseptic isolation cabinet 100 includes a cup separating treatment bin 110, an extraction liquid separating bin 120 and a transfer channel 130, wherein the cup separating treatment bin 110 is internally provided with a cup separating treatment mechanism 300 and an extraction plate 200; the nucleic acid extraction mechanism 400, the amplification plate 500 and the liquid and sample separation mechanism 600 are arranged in the extraction and separation bin 120, and the air pressure of the extraction and separation bin 120 is greater than the air pressure in the separation cup treatment bin 110; the transfer channel 130 is disposed between the cup separation processing bin 110 and the extraction liquid separation bin 120, and is used for communicating the cup separation processing bin 110 and the extraction liquid separation bin 120. The nucleic acid detecting pretreatment device further comprises an extraction plate transfer mechanism 700, wherein the extraction plate transfer mechanism 700 is arranged in the aseptic isolation box and is used for transferring the extraction plate 200 carrying the sample from the cuvette processing bin 110 to the extraction and liquid separation bin 120 through the transfer channel 130.
In this embodiment, the operation space in the aseptic isolation cabinet 100 is divided into the separate cuvette processing chamber 110 and the extraction cuvette processing chamber 120, the sample is in the cuvette processing chamber 110 and is in the cuvette processing chamber 200 by the cuvette processing mechanism 300, the extraction plate transfer mechanism 700 transfers the extraction plate 200 with the sample to the extraction cuvette chamber 120 through the transfer channel 130, and then the nucleic acid extraction mechanism 400 in the extraction cuvette 120 extracts the nucleic acid from the sample on the extraction plate 200 and transfers the sample after the nucleic acid extraction to the amplification plate 500. Because the air pressure of the extracting and separating bin 120 is greater than the air pressure in the separating cup processing bin 110, when the extracting and separating bin 120 is communicated with the separating cup processing bin 110 through the transit channel 130, namely, when the extracting plate 200 is transferred, the air in the extracting and separating bin 120 flows into the separating cup processing bin 110, so that the aerosol generated in the separating cup processing bin 110 can be effectively prevented from polluting the extracting and separating bin 120, the sectional setting of a pollution area and a semi-pollution area can be realized, and the extracting and separating bin 120 is a semi-pollution area.
Alternatively, the extraction plate transfer mechanism 700 may be a robot or a conveyor mechanism, etc., which is not limited by the embodiment of the present application.
In one possible implementation manner, the nucleic acid detection pretreatment device further comprises a first air inlet filter device, a second air inlet filter device and a first air outlet filter device, wherein the first air inlet filter device is arranged at one side of the bottom of the cup separation treatment bin 110 far away from the extraction liquid separation bin 120 and is used for filtering external gas and inputting the filtered external gas into the cup separation treatment bin 110; the second air inlet filter is arranged at one side of the bottom of the cup separating treatment bin 110, which is close to the extracting and separating bin 120, and is used for filtering external air and inputting the filtered external air into the cup separating treatment bin 110; the first exhaust filtering device is arranged at the top of the cup separating treatment bin 110 and is used for filtering and discharging the gas in the cup separating treatment bin 110.
In this implementation manner, the first air inlet filter device and the second air inlet filter device are respectively arranged at two sides of the bottom of the cup separating treatment bin 110, air enters the cup separating treatment bin 110 after being filtered by the first air inlet filter device and the second air inlet filter device, and the air in the cup separating treatment bin 110 is discharged out of the sterile isolation box body 100 through the first air outlet filter device, so that an air passage is formed between the first air inlet filter device and the first air outlet filter device, and another air passage is formed between the second air inlet filter device and the first air outlet filter device, and further, the rear end of a pollutant stream such as virus aerosol at the front end of the cup separating treatment bin 110 can be effectively reduced, and cross contamination is formed.
Alternatively, the inspector can adjust the air pressure within the cup separation treatment chamber 110 by adjusting the power of the first inlet filter, the second inlet filter, and the first outlet filter.
In one possible implementation manner, the nucleic acid detecting pretreatment device further comprises a third air inlet filter device, a fourth air inlet filter device and a second air outlet filter device, wherein the third air inlet filter device is arranged at one side, close to the cup separating treatment bin 110, of the bottom of the extraction and liquid separating bin 120, and is used for filtering external air and inputting the filtered external air into the extraction and liquid separating bin 120; the fourth air inlet filter is arranged at one side of the bottom of the extraction and liquid separation bin 120 far away from the cup separation processing bin 110, and is used for filtering external air and inputting the filtered external air into the extraction and liquid separation bin 120; the second exhaust filtering device is disposed at the top of the extraction and separation chamber 120, and is configured to filter and discharge the gas in the extraction and separation chamber 120.
In this implementation manner, the third air inlet filter device and the fourth air inlet filter device are respectively arranged at two sides of the bottom of the extraction and liquid separation bin 120, air enters the extraction and liquid separation bin 120 after being filtered by the third air inlet filter device and the fourth air inlet filter device, and air in the extraction and liquid separation bin 120 is discharged out of the sterile isolation box body 100 through the second air outlet filter device, so that an air passage is formed between the third air inlet filter device and the first air outlet filter device, another air passage is formed between the fourth air inlet filter device and the second air outlet filter device, and further, the back end of a pollutant stream such as virus aerosol at the front end of the extraction and liquid separation bin 120 can be effectively reduced, and cross contamination is formed.
In one possible implementation, a first door 131 is disposed between the transfer channel 130 and the cup separating processing bin 110, and the first door 131 is configured to be opened when the extraction plate 200 enters the transfer channel 130 and closed after the extraction plate 200 enters the transfer channel 130; a second bin gate 132 is disposed between the transfer channel 130 and the extraction and separation bin 120, and the second bin gate 132 is configured to be opened after the extraction plate 200 enters the transfer channel 130 and the first bin gate 131 is closed, and to be closed after the extraction plate 200 enters the extraction and separation bin 120. The nucleic acid detecting pretreatment device further includes a first automatic sterilizing device for sterilizing the transfer passage 130 when the extraction plate 200 enters the transfer passage 130 and the first and second gates 131 and 132 are closed.
In this implementation, after the sample is separated into the samples and applied to the extraction plate 200 in the separation processing bin 110, the first bin gate 131 is opened, the extraction plate transfer mechanism 700 transfers the extraction plate 200 with the sample into the transfer channel 130 through the first bin gate 131, then the first bin gate 131 is closed, at this time, the transfer channel 130 is in a closed state, and the first automatic sterilizing device automatically opens the transfer channel 130 to sterilize the transfer channel 130, and simultaneously, also kills the extraction plate 200 in the transfer channel 130. After the sterilization is completed, the second door 132 is opened, and the extraction plate transfer mechanism 700 moves the extraction plate 200 from the transfer passage 130 into the extraction and separation chamber 120, and at this time, the air pressure in the transfer passage 130 is equal to the air pressure in the extraction and separation chamber 120. Because the air pressure in the transfer channel 130 is equal to the air pressure in the extracting and separating bin 120, and the air pressure in the extracting and separating bin 120 is greater than the air pressure in the separating cup processing bin 110, when the first bin door 131 is opened, the air in the transfer channel 130 flows to the separating cup processing bin 110, so that the pollution source in the separating cup processing bin 110 is prevented from entering the transfer channel 130. At this time, the gas in the transfer channel 130 is reduced by less than the gas pressure in the extraction and separation chamber 120, and when the second chamber door 132 is opened, the gas in the extraction and separation chamber 120 flows in the transfer channel 130, so that the pollution source in the transfer channel 130 can be prevented from entering the extraction and separation chamber 120. Therefore, the application can effectively prevent the pollution source in the cup separating treatment bin 110 from entering the extraction liquid separating bin 120 and effectively isolate the cup separating treatment bin 110 from the extraction liquid separating bin 120 through the switch cooperation between the first automatic sterilizing device and the first bin gate 131 and the second bin gate 132.
In one possible implementation, the air pressure within the sub-cup processing chamber 110 is less than the ambient atmospheric pressure.
In this implementation manner, when the sample is transported from the outside into the sub-cup processing chamber 110, the air flow direction from the outside into the sub-cup processing chamber 110 is the direction from the outside to the inside of the sub-cup processing chamber 110 because the air pressure in the sub-cup processing chamber 110 is smaller than the outside air pressure, so that the leakage of the contamination source such as virus aerosol in the sub-cup processing chamber 110 can be effectively prevented.
In one possible implementation, the aseptic isolation tank 100 further includes a sample input channel 140 for communicating the sub-cup processing chamber 110 with the outside, and a third chamber door 141 is provided at an end of the sample input channel 140 that communicates with the outside, and the third chamber door 141 is configured to be opened when the sample is transferred from the outside to the sample transfer chamber, and to be closed after the sample enters the sample transfer chamber; a fourth bin gate 142 is arranged between the sample input channel 140 and the cup separating processing bin 110, and the fourth bin gate 142 is used for being opened after the sample enters the sample conveying bin and the third bin gate 141 is closed, and is closed after the extraction plate 200 enters the extraction and liquid separating bin 120; the device further comprises a sample delivery mechanism 800 and a second automatic killing device, the sample delivery mechanism 800 being disposed within the sample input channel 140 or the cuvette handling bin 110 for moving the sample from the sample input channel 140 to the cuvette handling bin 110; the second automatic sterilizing device is used for sterilizing the sample delivery bin after the sample enters the sample delivery bin and the third and fourth bin gates 141 and 142 are closed.
In this implementation, the process starts, the third gate 141 is opened, the sample enters the sample input channel 140 from the outside through the third gate 141, then the third gate 141 is closed, the sample input channel 140 is in a closed state, and the second automatic sterilization device is started to sterilize the sample input channel 140 and the sample in the sample input channel 140. After the sterilization is completed, the fourth door 142 is opened, the sample is transferred into the sub-cup processing chamber 110 by the sample transfer mechanism 800, and finally, the fourth door 142 is closed, and the air pressure in the sample input channel 140 is the same as the air pressure in the sub-cup processing chamber 110. When the third door 141 is opened, the air pressure in the sample input channel 140 is the same as the air pressure in the sub-cup processing chamber 110, and the air pressure in the sub-cup processing chamber 110 is less than the external air pressure, so that the external air flows into the sample input channel 140, and the leakage of the virus aerosol and other pollution sources in the sample input channel 140 can be effectively avoided.
In one possible implementation, the extraction and separation cartridge 120 has a pressure less than the ambient atmospheric pressure.
Alternatively, the sample delivery mechanism 800 may be a robotic arm or a conveyor belt mechanism, etc., which is not limited in this embodiment of the application.
In the above implementation process, when the amplification plate 500 is transported from the extraction and separation chamber 120 to the outside, the air pressure of the extraction and separation chamber 120 is smaller than the external atmospheric pressure, and the air flow direction is from the outside into the extraction and separation chamber 120, so that the leakage of the virus aerosol and other pollution sources in the extraction and separation chamber 120 can be effectively prevented.
In one possible implementation, the aseptic isolation tank 100 further includes an amplification plate output channel 150 for communicating the extraction and separation chamber 120 with the outside, a fifth gate 151 being provided between the amplification plate output channel 150 and the extraction and separation chamber 120, the fifth gate 151 being configured to be opened when the amplification plate 500 is conveyed from the extraction and separation chamber 120 to the amplification plate output channel 150, and to be closed after the amplification plate 500 enters the amplification plate output channel 150; a sixth bin gate 152 is arranged at one end of the amplification plate output channel 150, which is communicated with the outside, and the sixth bin gate 152 is used for being opened after the amplification plate 500 enters the amplification plate output channel 150 and the fifth bin gate 151 is closed, and is closed after the amplification plate 500 enters the outside; the nucleic acid detection pretreatment device further comprises an amplification plate output mechanism 900 and a third automatic killing device, wherein the amplification plate output mechanism 900 is arranged in the amplification plate output channel 150 or the extraction and separation bin 120 and is used for transferring the amplification plate 500 from the extraction and separation bin 120 to the amplification plate output channel 150; the third automatic sterilizing device is used for sterilizing the amplification plate output channel 150 after the amplification plate 500 enters the amplification plate output channel 150 and the fifth and sixth gates 151 and 152 are closed.
In this embodiment, after the sample is separated into samples in the aseptic isolation cabinet 100 by separating the samples in a cup, extracting the nucleic acid, and separating the samples in a liquid into the amplification plates 500, the fifth door 151 is opened, the amplification plate output mechanism 900 transfers the amplification plates 500 with the processed sample to the amplification plate output channel 150, and then the fifth door 151 is closed, and at this time, the amplification plate output channel 150 is in a closed state, and the third automatic sterilizing device is automatically opened to sterilize the amplification plate output channel 150 and the amplification plates 500 in the amplification plate output channel 150. After the sterilization is completed, the sixth door 152 is opened, and the inspector takes out the amplification plate 500, at which time the pressure of the amplification plate output channel 150 is the same as the outside. In the process of outputting the amplification plate 500, when the fifth bin gate 151 is opened, since the pressure of the amplification plate output channel 150 is the same as the outside, and the air pressure of the extraction and separation chamber 120 is less than the outside air pressure, at this time, the air in the amplification plate output channel 150 flows into the extraction and separation chamber 120, and the air pressure in the amplification plate output channel 150 is reduced and is less than the outside air pressure, when the sixth bin gate 152 is opened, the air flow direction flows from the outside to the amplification plate output channel 150, and thus the leakage of the pollution source in the extraction and separation chamber 120 can be effectively avoided. Therefore, the third automatic sterilizing device is arranged on the amplification plate output channel 150 and is matched with the switch of the fifth bin gate 151 and the sixth bin gate 152, so that the leakage of a pollution source in the extracting and separating bin 120 can be effectively avoided.
Alternatively, the amplification plate output mechanism 900 may be a robotic arm, a conveyor belt mechanism, or the like, which is not limited in this embodiment of the application.
In one possible implementation, the aseptic isolation tank 100 further includes a first material input channel 160 for communicating the outside world with the sub-cup 110; a seventh bin gate 161 is arranged at one end of the first material input channel 160, which is communicated with the outside, and the seventh bin gate 161 is used for being opened when the extraction plate 200 is conveyed into the first material input channel 160 from the outside, and being closed after the extraction plate 200 enters the first material input channel 160; an eighth bin gate 162 is arranged at one end of the first material input channel 160, which is communicated with the sub-cup processing bin 110, and the eighth bin gate 162 is used for being opened after the extraction plate 200 enters the first material input channel 160 and the seventh bin gate 161 is closed, and is closed after the extraction plate 200 enters the sub-cup processing bin 110; the nucleic acid detection pretreatment device further comprises a first material input mechanism and a fourth automatic sterilization device, wherein the first material input mechanism is arranged in the first material input channel 160 and is used for conveying the extraction plate 200 from the outside into the cup separating treatment bin 110; the fourth automatic sterilizing apparatus is disposed in the first material input channel 160, and is used for sterilizing the first material input channel 160 after the extraction plate 200 enters the first material input channel 160 and the seventh bin gate 161 and the eighth bin gate 162 are closed simultaneously.
In the above implementation process, when the extraction plate 200 needs to be transported into the cup separating processing bin 110, the seventh bin gate 161 is opened, the extraction plate 200 enters the first material input channel 160 from the seventh bin gate 161, then the seventh bin gate 161 is closed, at this time, the first material input channel 160 is in a closed state, and the fourth automatic sterilizing device is automatically opened to sterilize the first material input channel 160 and the extraction plate 200 therein. After the sterilization is completed, the eighth bin gate 162 is opened and the first material input mechanism moves the extraction plate 200 into the cup separation processing bin 110. Therefore, the fourth sterilizing device and the seventh bin gate 161 and the eighth bin gate 162 are matched to effectively prevent the pollution source in the split cup treatment bin 110 from leaking out through the first material input channel 160.
Alternatively, the first material input mechanism is a conveyor mechanism extending through the first material input channel 160.
In one possible implementation, the aseptic isolation tank 100 further includes a second material input channel 170 for communicating the outside with the extraction and separation chamber 120, and a ninth door 171 is provided at an end of the second material input channel 170 communicating with the outside, and the ninth door 171 is configured to be opened when the amplification plate 500 is transferred from the outside into the second material input channel 170 and to be closed after the amplification plate 500 enters the second material input channel 170; a tenth bin gate 172 is arranged at one end of the second material input channel 170, which is communicated with the extraction and separation bin 120, and the tenth bin gate 172 is used for being opened after the amplification plate 500 enters the second material input channel 170 and the ninth bin gate 171 is closed, and is closed after the amplification plate 500 enters the extraction and separation bin 120; the device also comprises a second material input mechanism and a fifth killing device, wherein the second material input mechanism is arranged in the second material input channel 170 and is used for conveying the amplification plate 500 from the outside into the extraction and liquid separation bin 120; the fifth sterilizing device is disposed in the second material input passage 170 for sterilizing the second material input passage 170.
In the above implementation process, when the amplification plate 500 needs to be transported into the extraction and separation chamber 120, the ninth chamber door 171 is opened, the extraction plate 200 enters the second material input channel 170 from the ninth chamber door 171, then the ninth chamber door 171 is closed, at this time, the second material input channel 170 is in a closed state, and the fifth automatic sterilizing device is automatically opened to sterilize the second material input channel 170 and the amplification plate 500 therein. After the sterilization is completed, the tenth compartment door 172 is opened, and the second material input mechanism transfers the amplification plate 500 into the extraction and separation compartment 120. It can be seen that the present application can effectively prevent the leakage of the contamination source in the extraction and separation tank 120 through the second material input channel 170 by providing the fifth sterilizing device in cooperation with the ninth and tenth tank gates 171 and 172.
Alternatively, the second material input mechanism is a conveyor mechanism extending through the first material input channel 160.
Alternatively, the seventh bin gate 161 and the ninth bin gate 171 may be the same bin gate, that is, the first material input channel 160 and the second material input channel 170 may be combined into one material input channel, where the material input channel includes a bin gate in communication with the outside, a bin gate in communication with the cup separating processing bin 110, and a bin gate in communication with the extracting and separating bin 120, which may simplify the structure of the aseptic processing tank.
In one possible implementation, the aseptic isolation tank 100 further includes a first waste output channel 180, one end of the first waste output channel 180 is communicated with the cup separation treatment bin 110, and the other end is opened on the outer surface of the aseptic isolation tank; the nucleic acid detecting pretreatment device further includes a first waste output pipe, one end of which is communicated with the first waste output channel 180, and the other end of which extends to a first predetermined position.
In the above implementation process, the waste in the cup separation treatment bin 110 reaches the first waste output pipe through the first waste output channel 180, and is then guided out to the first predetermined position through the first waste output pipe, optionally, the first predetermined position is located outside the laboratory where the device is located before the nucleic acid detection treatment, so that the pollution level inside the laboratory where the device is located before the nucleic acid detection treatment can be reduced, and the protection level of the detection personnel is further reduced.
In one possible implementation, the aseptic isolation tank 100 further includes a second waste output channel 190, where one end of the second waste output channel 190 is in communication with the extraction and separation tank 120, and the other end is open on the outer surface of the aseptic isolation tank; the nucleic acid detecting pre-processing apparatus further includes a second waste output pipe, one end of which communicates with the second waste output channel 190, and the other end extends to a second predetermined position.
In the above implementation process, the waste in the extracting and separating bin 120 reaches the second waste output pipe through the second waste output channel 190, and is then guided out to the second predetermined position through the second waste output pipe, where the second predetermined position is optionally located outside the laboratory where the device is located before the nucleic acid detection processing, so as to reduce the pollution level inside the laboratory where the device is located before the nucleic acid detection processing, thereby reducing the protection level of the detection personnel.
Alternatively, the first waste output pipe and the second waste output pipe may be the same output pipe, that is, the ports of the first waste output channel 180 and the second waste output channel 190 formed on the outer surface of the aseptic isolation tank are the same port, so that the structure of the aseptic processing tank can be simplified.
The above description is only an example of the present application and is not intended to limit the scope of the present application, and various modifications and variations will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
The foregoing is merely illustrative of the present application, and the present application is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Claims (11)
1. A nucleic acid detection pretreatment device, comprising:
a sterile isolation box;
the extraction plate is arranged in the sterile isolation box body;
the cup separating processing mechanism is arranged in the sterile isolation box body and is used for separating the samples conveyed into the sterile isolation box body into cups and samples on the extraction plate;
the nucleic acid extraction mechanism is arranged in the sterile isolation box body and is used for extracting nucleic acid from the sample on the extraction plate;
the amplification plate is arranged in the sterile isolation box body;
the liquid separating and sampling mechanism is arranged in the sterile isolation box body and is used for separating the samples extracted by the nucleic acid on the extraction plate into liquid on the amplification plate;
the aseptic isolation tank includes:
the cup separating treatment bin is internally provided with the cup separating treatment mechanism and the extraction plate;
the nucleic acid extraction mechanism, the amplification plate and the liquid and sample separation mechanism are arranged in the extraction and liquid separation bin, and the air pressure of the extraction and liquid separation bin is larger than the air pressure in the cup separation treatment bin;
the transfer channel is arranged between the cup separation treatment bin and the extraction liquid separation bin and is used for communicating the cup separation treatment bin and the extraction liquid separation bin;
The device also comprises an extraction plate transfer mechanism, wherein the extraction plate transfer mechanism is arranged in the sterile isolation box and is used for transferring the extraction plate carrying a sample from the cup separation treatment bin to the extraction and liquid separation bin through the transfer channel;
a first bin gate is arranged between the transfer channel and the cup separating treatment bin, and is used for being opened when the extraction plate enters the transfer channel and closed after the extraction plate enters the transfer channel;
a second bin gate is arranged between the transfer channel and the extraction liquid separation bin, and is used for being opened after the extraction plate enters the transfer channel and the first bin gate is closed, and is closed after the extraction plate enters the extraction liquid separation bin;
the device also comprises a first automatic killing device, wherein the first automatic killing device is used for killing the transfer channel when the extraction plate enters the transfer channel and the first bin gate and the second bin gate are closed.
2. The apparatus as recited in claim 1, further comprising:
the first air inlet filter device is arranged at one side of the bottom of the cup separating treatment bin, which is far away from the extracting and liquid separating bin, and is used for filtering external air and inputting the filtered external air into the cup separating treatment bin;
The second air inlet filter device is arranged at one side of the bottom of the cup separating treatment bin, which is close to the extracting and liquid separating bin, and is used for filtering external air and inputting the filtered external air into the cup separating treatment bin;
the first exhaust filtering device is arranged at the top of the cup separating treatment bin and is used for filtering and discharging the gas in the cup separating treatment bin.
3. The apparatus as recited in claim 1, further comprising:
the third air inlet filter device is arranged at one side of the bottom of the extraction and liquid separation bin, which is close to the cup separation treatment bin, and is used for filtering external air and inputting the filtered external air into the extraction and liquid separation bin;
the fourth air inlet filter device is arranged at one side of the bottom of the extraction and liquid separation bin far away from the cup separation treatment bin and is used for filtering external air and inputting the filtered external air into the extraction and liquid separation bin;
and the second exhaust filtering device is arranged at the top of the extraction and liquid separation bin and is used for filtering and discharging the gas in the extraction and liquid separation bin.
4. A device according to any one of claims 1-3, wherein the air pressure in the sub-cup treatment chamber is less than the ambient air pressure.
5. The device according to claim 4, wherein the aseptic isolation box further comprises a sample input channel for communicating the cuvette handling bin with the outside, and a third bin gate is arranged at one end of the sample input channel, which communicates with the outside, and is used for being opened when a sample is conveyed from the outside to the sample conveying bin and closed after the sample enters the sample conveying bin;
A fourth bin gate is arranged between the sample input channel and the cup separating treatment bin, and is used for being opened after a sample enters the sample conveying bin and the third bin gate is closed, and is closed after the extraction plate enters the extraction liquid separating bin;
the device also comprises a sample conveying mechanism and a second automatic killing device, wherein the sample conveying mechanism is arranged in the sample input channel or the sub-cup treatment bin and is used for conveying a sample from the sample input channel to the sub-cup treatment bin; the second automatic sterilizing device is used for sterilizing the sample conveying bin after the sample enters the sample conveying bin and the third bin gate and the fourth bin gate are closed.
6. A device according to any one of claims 1-3, wherein the extraction and separation cartridge has a gas pressure less than the ambient atmospheric pressure.
7. The apparatus of claim 6, wherein the aseptic isolation tank further comprises an amplification plate output channel for communicating the extraction and separation chamber with the outside, a fifth door being disposed between the amplification plate output channel and the extraction and separation chamber, the fifth door being adapted to open when the amplification plate is transported from the extraction and separation chamber to the amplification plate output channel and to close after the amplification plate enters the amplification plate output channel;
A sixth bin gate is arranged at one end, communicated with the outside, of the amplification plate output channel, and is used for being opened after the amplification plate enters the amplification plate output channel and the fifth bin gate is closed, and is closed after the amplification plate enters the outside;
the device also comprises an amplification plate output mechanism and a third automatic killing device, wherein the amplification plate output mechanism is arranged in the amplification plate output channel or the extraction and liquid separation bin and is used for moving the amplification plate from the extraction and liquid separation bin to the amplification plate output channel; the third automatic sterilizing device is used for sterilizing the amplification plate output channel after the amplification plate enters the amplification plate output channel and the fifth bin gate and the sixth bin gate are closed.
8. A device according to any one of claims 1-3, wherein the aseptic isolation tank further comprises a first material inlet channel for communicating the outside with the sub-cup treatment bin; a seventh bin gate is arranged at one end, communicated with the outside, of the first material input channel, and is used for being opened when the extraction plate conveys the extraction plate from the outside into the first material input channel and closed after the extraction plate enters the first material input channel;
An eighth bin gate is arranged at one end of the first material input channel, which is communicated with the cup separating treatment bin, and is used for being opened after the extraction plate enters the first material input channel and the seventh bin gate is closed, and is closed after the extraction plate enters the cup separating treatment bin;
the device also comprises a first material input mechanism and a fourth automatic killing device, wherein the first material input mechanism is arranged in the first material input channel and is used for conveying the extraction plate from the outside into the cup separation treatment bin; the fourth automatic sterilizing device is arranged in the first material input channel and is used for sterilizing the first material input channel after the extraction plate enters the first material input channel and the seventh bin gate and the eighth bin gate are closed simultaneously.
9. A device according to any one of claims 1-3, wherein the aseptic isolation tank further comprises a second material inlet channel for communicating the outside with the draw-separating chamber, the second material inlet channel being provided with a ninth door at an end thereof communicating with the outside, the ninth door being adapted to open when the amplification plate is transported from the outside into the second material inlet channel and to close after the amplification plate enters the second material inlet channel;
A tenth bin gate is arranged at one end of the second material input channel, which is communicated with the extraction and liquid separation bin, and is used for being opened after the amplification plate enters the second material input channel and the ninth bin gate is closed, and is closed after the amplification plate enters the extraction and liquid separation bin;
the device also comprises a second material input mechanism and a fifth killing device, wherein the second material input mechanism is arranged in the second material input channel and is used for conveying the amplification plate from the outside into the extraction and liquid separation bin; the fifth sterilizing device is arranged in the second material input channel and is used for sterilizing the second material input channel.
10. A device according to any one of claims 1 to 3, wherein the aseptic isolation tank further comprises a first waste output channel, one end of which is in communication with the sub-cup treatment bin and the other end of which is open on the outer surface of the aseptic isolation tank;
the device further comprises a first waste output pipe, one end of the first waste output pipe is communicated with the first waste output channel, and the other end of the first waste output pipe extends to a first preset position.
11. A device according to any one of claims 1-3, wherein the aseptic isolation tank further comprises a second waste output channel, one end of which is in communication with the extraction and separation bin and the other end of which is open on the outer surface of the aseptic isolation tank;
the device further comprises a second waste output pipe, one end of the second waste output pipe is communicated with the second waste output channel, and the other end of the second waste output pipe extends to a second preset position.
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